CN206387811U - The device of analyte in a kind of detection sample - Google Patents
The device of analyte in a kind of detection sample Download PDFInfo
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- CN206387811U CN206387811U CN201620807353.7U CN201620807353U CN206387811U CN 206387811 U CN206387811 U CN 206387811U CN 201620807353 U CN201620807353 U CN 201620807353U CN 206387811 U CN206387811 U CN 206387811U
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Abstract
The utility model provides a kind of device for detecting analyte in sample, and the device includes one end open, the passage of one end closing, wherein the passage is used to set detection reagent bar, wherein, passage includes flood control flow structure, and the structure allows the cross-sectional area of passage to diminish at flood control flow structure.By device, the mighty torrent phenomenon of test agent bar can be prevented, error rate is reduced.
Description
Technical field
The utility model is related to the detection reagent in a kind of carrier of containing detection reagent strip, especially quick diagnosis field
The storing apparatus of bar.
Background technology
Be presently used for detection sample in whether the detection means containing analyte, be largely used to hospital or family
In, these detection means for being applied to quick diagnosis include one or more detection reagent bars, such as early pregnancy is detected, drug abuse
Detection etc..The detection means of this quick diagnosis is facilitated very much, can be at one minute, or at most ten minutes or so are detecting
Testing result is obtained on reagent strip.
In these detection means, detection reagent bar is normally on certain carrier.Carrier can be the mould of plate shape
Or plate etc..Carrier is typically made up of neck or passage, and a neck or passage can accommodate a detection reagent bar,
So the carrier with detection reagent bar can be used alone, and can also be used cooperatively with container.When using such carrier come
When containing detection reagent strip, can be used directly to whether contain material interested, this when, sample in detection sample
Can be liquid sample, such as urine.It is inserted into when the carrier with detection reagent bar in sample, or allows liquid sample stream
When entering into the container with carrier, because the flowing that liquid enters in carrier is not smooth flow, but mixed and disorderly nothing
Chapter, usually pour in a short period of time neck either in passage or containing detection reagent strip intracavitary, so,
Commonly referred to as (flooding) " mighty torrent " phenomenon can be caused, so as to influence normal work and the normal reaction of detection reagent bar.
It is improved it is necessary to the carrier to traditional carrying detection reagent bar, so that such as mighty torrent phenomenon is avoided,
So as to improve the accuracy and validity of detection reagent bar.
Utility model content
In order to solve problems of the prior art, the utility model provides such a carrier, and the carrier includes using
In the passage of containing detection reagent strip, passage end closing, the other end has opening, and detected fluid sample can pass through
The opening is entered in passage, wherein, flood control flow structure is included in the passage.Or, the utility model provides a kind of passage, should
Passage, passage end closing, the other end has opening, and detected fluid sample can enter passage by the opening
In, wherein, include the structure of anti-mighty torrent in the passage.It is preferred that, the liquid into passage is contacted with detection reagent bar, preferably
, contacted with the sample application zone of detection reagent bar, or, simply contacted with the sample application zone of detection reagent bar.
In some preferred modes, the flood control flow structure is located at the vicinity of the opening of passage.
In some preferred modes, flood control flow structure is located under the marked region of detection reagent bar or away from detection
Reagent strip marked region.In some preferred modes, flood control flow structure is located near the fluid applying zone of detection reagent bar
Or the marked region with detection reagent bar is not in same level position.It is preferred that, flood control flow structure is located at reagent strip
Near waterline, it is furthermore preferred that flood control flow structure is located on the waterline of reagent strip, under marked region.
In some preferred modes, flood control flow structure is included in protruding component in passage, and the protruding component allows part
The cross-sectional area of passage is reduced.In some preferred modes, protruding component is respectively on the two side of cavity, and to passage
Protrusions.It is preferred that, flood control flow structure allows part to allow the cross-sectional area of passage portion to reduce, or allows passage portion to become narrow.
In some preferred modes, the opening cross-sectional area of passage is more than containing the crosscutting of the passage at flood control flow structure
Area.In some preferred modes, the width of the opening of passage is more than the width of channel interior.
In some preferred modes, passage is divided into two parts by flood control flow structure, and a part is that flood control flow structure is arrived
The first paragraph passage of access portal, another part is exactly to control flood flow structure to one end second segment passage of closed channel, wherein, the
The cross-sectional area of one section of passage is more than the cross-sectional area of second segment passage;Or, the cross section of the passage portion of first paragraph passage
Passage portion cross-sectional area of the product more than second segment passage;Or, the cross-sectional area of each part of first paragraph passage is both greater than
The cross-sectional area of each part of second segment passage.The intersection of first paragraph passage and the second end passage forms of the present utility model
Flood control flow structure, or the intersection of first paragraph passage and the second end passage become narrow by passage.It is preferred that, first paragraph passage with
The intersection of second end passage is one and gradually becomes narrow region, allows passage to become narrower at the region.Form this practicality
New flood control flow structure, when passage is cylinder, can weigh the size of passage with the cross-sectional area of passage.When
When passage is rectangle or cuboid, it is highly consistent in the case of, the bigger place of the width of passage, overall cross section
Area is bigger, so, can also be first paragraph passage width be more than second segment passage width, wherein, first passage and
The height of second channel is the same.
It is preferred that, it is more than the width of channel restriction, but the height at the opening of passage in the width of the opening of passage
Spend the height also greater than channel restriction.
In some preferred modes, passage includes bottom plate and notch, and wherein bottom plate and notch forms one end
Opening, the sealed passage in one end, wherein, opening is near the sample applying zone of test-strips.It is preferred that, neck includes narrow
Neck is divided into two parts by structure, the narrow structures, and the wherein width at narrow structures is less than the width at the other positions of neck.
It is preferred that, an elongated sulculus is included at the top of neck, the sulculus runs through whole passage, or through the top of whole neck
Portion.
In some preferred modes, detection reagent bar can also be included in cavity lane, the sample of the reagent strip applies
Region be located at access portal vicinity, sample absorbance region positioned at channel enclosed one end or near.In some preferred sides
In formula, the marked region on reagent strip is located in passage near flood control flow structure.
In foregoing all embodiments, it is transparent flat that the passage for containing detection reagent strip on carrier includes one
Hardened structure and notch, wherein, described notch peace is hardened to be configured to described passage.It is preferred that, notch
To be nontransparent.In some preferred modes, the detection zone of detection reagent bar, Huo Zhejian can be seen through slab construction
The detection zone of test agent bar face transparent plate structure or with transparent plate structure it is facing set.
On the other hand, the utility model provides a kind of device for detecting analyte in sample matter, wherein the device bag
Foregoing described carrier or passage are included, the carrier includes the passage for containing detection reagent strip, and the passage end is closed, separately
One end has opening, and detected fluid sample can be entered in passage by the opening, wherein, flood control is included in the passage
Flow structure.In some preferred modes, the device also includes detection reagent bar, wherein, the reagent strip is located at described passage
In.The mode of foregoing all flood control flow structures may be included within detection means of the present utility model.It is preferred that, anti-mighty torrent
Structure is located under the marked region on detection reagent bar, or, flood control flow structure is located at the marked region on detection reagent bar
Between sample areas;Or, the marked region on reagent strip is located on flood control flow structure.
In a preferred mode, described device also includes the cavity of a receiving liquid sample, wherein, the carrier
In the cavity, wherein, the described sample applying zone for checking reagent strip is close to the bottom of cavity, so, the liquid of cavity
Body sample can just be entered by the opening of passage in passage come, contacted with the sample applied area of the reagent strip in passage so that
Liquid is with reagent strip from sample applied area sequentially through mark zone, detection zone, detection control area (if there is), most Zhongdao
Up to suction zones (if there is).
The utility model provides a kind of method for detecting fluid sample analyte, and this method includes:A kind of inspection is provided
Device is surveyed, the detection means includes carrier, and the carrier includes the passage for containing detection reagent strip, and the passage end is closed,
The other end has opening, and detected fluid sample can be entered in passage by the opening, wherein, include in the passage anti-
Mighty torrent structure, wherein, the passage includes detection reagent bar, wherein, flood control flow structure is located at the marked region of detection reagent bar
Between access portal;Allow fluid sample to enter by the opening of passage in passage, allow the sample applying zone of detection reagent bar
The fluid sample in passage is contacted, so as to examine the analyte in fluid sample.Or, flood control flow structure is located at detection reagent
Under the marked region of bar, on access portal.
In some preferred modes, the flood control flow structure is located at the vicinity of the opening of passage.
In some preferred modes, flood control flow structure is located under the marked region of detection reagent bar or away from detection
Reagent strip marked region.In some preferred modes, flood control flow structure is located near the fluid applying zone of detection reagent bar
Or the marked region with detection reagent bar is not in same level position.
In some preferred modes, flood control flow structure is included in the protruding component in passage, and the protruding component is by passage
Cross-sectional area reduce.In some preferred modes, protruding component is respectively on the two side of cavity, and to passage convexity
Rise.
In some preferred modes, the opening cross-sectional area of passage is more than containing the crosscutting of the passage at flood control flow structure
Area.In some preferred modes, the width of the opening of passage is more than the width of channel interior.
In some preferred modes, passage is divided into two parts by flood control flow structure, and a part is that flood control flow structure is arrived
The first paragraph passage of access portal, another part is exactly to control flood flow structure to one end second segment passage of closed channel, wherein, the
The cross-sectional area of one section of passage is more than the cross-sectional area of second segment passage.When passage is cylinder, passage can be used
Cross-sectional area weighs the size of passage.When passage be rectangle or cuboid when, it is highly consistent in the case of, passage
The bigger place of width, overall cross-sectional area is bigger, so, can also be that the width of first paragraph passage is more than second segment
The width of passage, wherein, first passage is as the height of second channel.
In some preferred modes, detection reagent bar can also be included in cavity lane, the sample of the reagent strip applies
Region be located at access portal vicinity, sample absorbance region positioned at channel enclosed one end or near.In some preferred sides
In formula, the marked region on reagent strip is located in passage near flood control flow structure.
Brief description of the drawings
Fig. 1 is principle schematic of the present utility model and is compared figure with the structure of conventional channels.Wherein, Figure 1A and Fig. 1 D are
The principle schematic of the utility model carrier, Figure 1B is the structural representation of traditional carrying detection reagent bar, and Fig. 1 C are detection
The structural representation of reagent strip;Fig. 1 E are that the combining structure of carrier structure and reagent strip in one embodiment of the utility model is shown
It is intended to.
Fig. 2 is that multiple passages are arranged on the carrier of the utility model one embodiment, and detection examination can be accommodated in the passage
The dimensional structure diagram of agent bar.
Fig. 3 is the amplification dimensional structure diagram of a channel design in Fig. 2;
Fig. 4 is the schematic perspective view for the notch for removing transparent plate structure;
Fig. 5 is the partial enlargement structural representation of a passage;
Fig. 6 A are the profilographs of a passage of another embodiment of the present utility model, and with Fig. 6 B be reagent strip
Rigging position schematic diagram;
Fig. 7 is that the liquid flowing containing reagent strip causes the principle schematic of mighty torrent phenomenon in conventional channels.
Fig. 8 is that the package assembly of the urine cup containing the utility model passage in one embodiment of the utility model is shown
It is intended to.
Embodiment
The structure being related to below to the utility model or these used technical terms are described further.
Detection
Detection represents chemical examination or tests a kind of material or material whether there is, such as, but is not limited to this, chemical substance,
The generation of organic compound, inorganic compound, metabolism product, medicine or drug metabolite, organic organization or organic organization
Thank thing, nucleic acid, protein or polymer.In addition, detection represents test substances or the quantity of material.Furtherly, table is gone back in chemical examination
Show immune detection, chemical detection, enzyme detection etc..
Sample
The signified sample of the utility model, which refers to those, to be divided for detection, chemical examination or diagnosis with the presence or absence of interested
Analyse the material of material.Sample can be, for example, fluid sample, it is liquid sample that fluid sample, which can be, and liquid sample can include
Blood, blood plasma, serum, urine, saliva and various juices, can also include solid sample and semi-solid samples by advance place
The liquid solution formed after reason.The sample that collection comes can be used for the methods such as immune detection, chemical detection, enzyme detection
It is no to there is analyte.
Detection reagent bar
The horizontal effluent reagent strip 500 that detection reagent bar of the present utility model can be known as is applied to, these inspections
The concrete structure and Cleaning Principle of test agent bar are technology known to persons skilled in the art in the prior art.Common
Detection reagent bar 500, including sample collecting zone 503, marked region 502, detection zone 504 and suction zone 501, sample are received
Collecting region includes sample receiving pad, and marked region 502 includes label pad, and suction zone 501 can include adsorptive pads, wherein detecting
Include that the necessary chemical substance containing analyte, such as immunoreagent or enzymology examination can be detected whether on region 504
Agent.Typically conventional detection reagent bar includes nitrocellulose filter for nitrocellulose assay strip, i.e. detection zone 504,
Specific binding molecule is fixed on nitrocellulose filter to show the result of detection;It can also be cellulose acetate film or nylon membrane etc.
Deng, certainly, the downstream of detection zone can also include testing result control area 505, generally, control area and detection zone
On in the form of horizontal line occur, be detection line or control line.Such detection reagent bar is traditional reagent strip, certainly,
But other other types of reagent strips detected using capillarity.In addition, carrying desiccation on general detection reagent bar
Learn agent formulations, such as fixed antibody or other reagents, after liquid is run into, liquid is as capillarity is along reagent strip
Flowing, with flowing, allows dry agent formulations to be dissolved in liquid, so as to occur to dry reagent of next regional processing in the area
Reaction, so as to carry out necessary detection.Liquid flowing is mainly carried out by capillarity.
Analyte
Some haptens materials, these haptens bags can be included with the example for the analyte being related in the utility model
Include drugs (such as drug abuse)." drug abuse " (DOA) refers to that the work of paralysis nerve (is generally played in non-medical destination using medicine
With).Abusing these medicines can cause body & mind to suffer damage, and produce dependence, addicted and/or death.Medicine is indiscriminate
Example includes cocaine;Amphetamine AMP is (for example, black beauty, white amphetamine tablet, dextroamphetamine, dextrorotation
Amphetamine tablet, Beans);Crystal methamphetamine MET (crank, meth, crystal, speed);Barbiturate
BAR (such as Valium, Roche Pharmaceuticals, Nutley, New Jersey);Sedative (paramedicines of sleeping);
Lysergic acid diethylamide (LSD);Inhibitor (downers, goofballs, barbs, blue devils, yellow
Jackets, methaqualone);The anti-antidepressant of tricyclic antidepressants (TCA, i.e. imipramine, amitriptyline and doxepin);Two methylenedioxy groups
Methylaniline MDMA;Hog (PCP);Tetrahydrocannabinol (THC, pot, dope, hash, weed, etc..);Opiate
(i.e. morphine MOP or, opium, cocaine COC;, heroin, hydroxyl dihydrocodeinone);Anxiolytic and hypnotic sedative agent, resist
Anxiety medicine is that a class is mainly used in anxiety reduction, anxiety, fear, is set the mind at rest, and has the medicine of hypnosis sedation, including benzene concurrently
Diazepine BZO (benzodiazepines), atypia BZ classes, fusion phenodiazine NB23C classes, the tall and erect class of benzene nitrogen, the part of BZ acceptors
Class, open loop BZ classes, diphenylmethane derivatives, piperazine carboxylic acid salt, piperidine carboxylic acid salt, Kui oxazoline ketone, thiazine and thiazole spread out
Biology, other heterocyclics, imidazole type calmness/anodyne (such as hydroxyl dihydrocodeinone OXY, methadone MTD), propane diols derive
Thing-carbamates, aliphatic compound, anthracene derivative etc..Inspection is can be used for using detection means of the present utility model
Survey and belong to medical usage but the easily detection of overdose, such as tricyclic antidepressant (imipramine or the like) and acetyl ammonia
Base phenol etc..These medicines can resolve into different small-molecule substances after being absorbed by the body, these small-molecule substances be present in blood,
In the body fluid such as urine, saliva, sweat or part body fluid has above-mentioned small-molecule substance.
Detection means
Detection means refer to for detect in sample whether the device containing analyte.In the utility model, inspection
Device is surveyed especially to represent for the detection means in quick diagnosis field, such as detection reagent bar 500, detection card or plate-inserting structure
100, test bar detects the various forms of detection means such as cup (shown in Fig. 8).Herein, reagent strip is inserted into detection card 100
In the passage 300 of (Fig. 2), wherein, make the sample applied area of test-strips exposed (in such as Fig. 5 and Fig. 7) from opening 305, thus
Detection card is formd, detection card can inherently be detected with complete independently.Such as Figure 1A, detection is illustrated with one of passage
Principle, when in use, detection card is inserted into liquid sample, because the passage end 12 of detection card is closed, the other end
Opening 11, such liquid sample is flowed into passage with regard to some, but is due to passage end closing, and the inflow of liquid is logical
Road is internally formed closed gas, so that under external and internal pressure, in liquid inlet channel after can only keep certain in the channel
Level height, as in the entrance passage of liquid and during being maintained at fixed level height, liquid just submerges reagent strip
Sample applying zone, liquid is flowed up due to the capillarity of reagent strip along reagent strip, so that detection is completed, if examination
There are lines or occurs without lines in the detection zone of agent bar, means that and whether there is analyte in sample.Figure 1B is biography
The passage detected using the principle and the installation diagram of reagent strip of system.
No matter the single reagent strip as Figure 1B carries out the detection of analyte in one or several kinds of liquid samples, also
It is that as Fig. 2, multiple detection test-strips are disposed in multiple different passages 300 (the basic phase of structure of each passage
Together), a kind of analyte of each detection reagent bar correspondence, is so directly inserted into detection card 100 in liquid sample, can be with
Once carry out the detection of multiple different analytes, such as 3,10,15 different analytes.It is, of course, also possible to such as
Like that, the card that passage or such as multiple reagent strips of Fig. 2 containing a reagent strip are disposed in multiple passages is disposed in Fig. 8
(such as generally in described urine cup), allowed in Fig. 8 container access portal one end positioned at the bottom 92 of urine cup, channel enclosed
One end 312 be located at close to vicinity of urine cup mouthfuls 91, so, when urine cup collects urine, as urine is into urine cup
In cavity 90, single channels operation principle as previously described is the same, and each passage is due to the effect of closed gas, each logical
There is the liquid (when inside and outside air pressure balance) of certain altitude in road, so as to complete the reaction of each reagent strip.
Detection means can block 20 matching used parts including detection card 20 and with detection, as shown in figure 8, urine cup 2
Can as detection means a kind of form.
In current detection, this cassette or cup type detection means is required for detection reagent bar 500 with one
Carrier 100 is supported, and the function of competence exertion detection, so this carrier can include multiple passages 300, in each passage
300 it is inner can include reagent strip 500, and each one or several kinds of analytes of reagent strip correspondence.Or, at least need
Want to set reagent strip in a passage, the passage.However, in existing traditional field, using passage principle (closed gas and
External and internal pressure) under detected, because reagent strip is located in passage, because cooperation or physics between reagent strip and passage
Position, often results in some many defects of reagent strip, a kind of defect such as patent patent customer service phenomenon of broken string.This is
Because the wall of detection reagent bar and passage is usually contacted closely, so that capillarity is produced, so as to cause broken string.Solution
Just it is to try to not allow detection reagent bar to contact with the wall of passage or bump contact, i.e., such as Figure 1B or Fig. 7 like that, allow
Passage and detection reagent bar have a certain distance, such as 1-4 millimeters of either farther distance or several millimeters of distance.
Although so can with it is relatively effective solve to break the problem of, therewith with the problem of, and it is maximum the problem of be exactly " flood
Stream " problem.Detected because no matter being directly inserted into the reagent strip in single passage in liquid sample, or detection means
(such as urine cup) includes multiple passages to carry out the detection of analyte in liquid sample, can all there is going out for mighty torrent phenomenon
It is existing.
So-called mighty torrent phenomenon, is exactly that within the of short duration time, substantial amounts of liquid-like 701 is gushed with reference to Fig. 7 further instructions
Enter onto reagent strip, allowing reagent strip 500 to have little time to carry out normally flowing by capillarity absorption sample, (561 liquid is
By the vestige of the flowing of normal capillarity), but more sample is rushed in test-strips or on surface (indicated by 560
Liquid), by the quick flow forward in the surface of test-strips, due to the stream of this not capillarity of the flowing of most liquid-like
Dynamic, but directly quickly flowed on surface, it is being that dry chemistry reagent on reagent strip is difficult quick in a short time so to handle
It is completely dissolved and is discharged into liquid sample, so, it is not in lines to have resulted in detection zone or detection control area.
Because the liquid on surface without or it is less flowed faster by capillarity, other portions of reagent strip have been moistened in advance
Point, therefore, allow reagent strip to lose main capillarity in advance, so, can hardly just allow and carry or be dissolved with reagent
The reagent of dry-cure is flowed further along on bar, so, although test strips, which are looked, to be wet, but without generation essence
Reaction, so as to will not be reacted in detection zone or control zone, it is inaccurate also to have resulted in testing result, will not sometimes go out
The situation of existing testing result.
As shown in Figure 7, in traditional device, reagent strip 500 is typically not directly contacted with that (most passages are carried with passage 300
Support frame), such reagent strip and passage are once inserted into liquid sample 700, because different pairs of operating personnel are different, are inserted
Depth, the speed of insertion entered, and different (the static liquid flowers for either whether flowing or splashing of the state 701 of liquid sample
701), when the passage containing test-strips enters in liquid sample, due to the influence of microenvironment in short-term, substantial amounts of liquid can be caused to exist
Poured in the very short time in passage, under microenvironment, liquid is poured in passage by access portal 305, due to logical
The one end 312 in road 300 is sealed, and the liquid of entrance just seals one section of gas with the end, so that pressure is produced to the liquid level of liquid,
Entered so as to limit more liquid in passage, be finally reached balance, allow liquid to be in certain height in passage.Although
Reach that the time of balance is very short, but in this process, liquid is in rambling and right in the state of microcosmic
Reagent strip produces many different directions, very big impulsive force, so as to cause so-called mighty torrent phenomenon.Same similar reason,
Passage containing reagent strip or card 100 are arranged in urine cup by static, when being injected into urine cup in liquid sample, for example, are urinated
Liquid, because liquid quickly flows in a short time, subsequently into passage, so that reach that the process of internal and external equilibrium is although of short duration,
But the liquid flowing under microenvironment is still fierce, so as to have partially liq directly from reagent strip surface flow, so as to make
Into mighty torrent phenomenon, so as to make testing result failure or test result inaccurate.Certainly, also have, because pouring into liquid into urine cup
The direction of body sample, angle injects the difference of the speed of liquid;Further, especially, when being detected, subject is straight
Connected genitals to inject in urine cup urine, urine cup usually contains the reagent strip with passage, in this case, due to
Different subjects does not have the operational capacity of specialty, urinates the angle into the urine to urine cup, and size is how many to wait also phase not to the utmost
Together, especially, it is in feeder connection in relative complex environment, test is allowed in a word under conditions of one is extremely complex,
So, it is easier to cause mighty torrent phenomenon, so as to influence final testing result.
In order to overcome this defect, the utility model can limit liquid using such carrier 100 or passage 300
Sample arbitrarily flows in passage, so as to reduce the generation of mighty torrent.Further, the structure of the flood control flow structure can quickly allow
Also liquid is in tranquility, either quickly reach internal and external equilibrium or, it is possible to reduce liquid is not made by the capillarys of test-strips
With the phenomenon of flowing.As analysis above, it is capillarity that reagent strip flows main effect by liquid, and the mighty torrent caused
Liquid is exactly allowed to f hardly pass through or seldom flowed by capillarity on reagent strip.
Carrier or passage
Carrier 100 or passage 300 refer to the part or element for containing detection reagent strip 500.Such as Figure 1A and Fig. 7
Shown, detection reagent bar 500 can be arranged in the passage 300 on carrier, one end closing 312 of the passage, another end opening
305, extraneous liquid is entered in passage by opening, when the upright placement of carrier 100 or horizontal positioned, detection examination
Agent bar 500 all typically drops out all without from carrier 100, concretely, and reagent strip will not take off from the passage 300 on carrier
Drop out and.Herein, carrier inherently passage, or passage inherently carrier a kind of form, detection is accommodated in passage
Reagent strip.According to this meaning, the utility model provides a kind of passage, passage end closing, another end opening, detection reagent
Bar is disposed in passage.It is preferred that, the sample applying zone 503 of detection reagent bar detects control zone close to the opening of passage
Domain is located at the blind end close to passage.The element of carrier 100 is constituted, can be the form of neck or the form of passage
300, or, neck and a slab construction assemble to form passage 300 of the present utility model.The number of neck or passage 300, can
To be 1,2,3,4 even more more.In general, a neck or passage 300 can accommodate a detection reagent
Bar 500.Typically, in one end of passage, one end of such as closing includes clamp structure, such knot in passage 300 or neck
Structure can clamp reagent strip.Certain clamp structure it is not necessary to structure, it is only a preferred technical scheme.
In some preferred modes, the structure of flood control flow structure is located in passage, and passage is divided into two not by the structure
With part, be for example divided into first passage and second channel, first passage is located at the opening of passage to the flow structure, second of controlling flood
Passage is from the position of flood control flow structure to the blind end of passage, and first passage and second channel intersection are flood control flow structure.
The structure of the limitation fluid reduces the cross-sectional area of passage or makes limitation fluidic structures that one part of passage formation is narrow and small
Space.In some specific modes, such as Figure 1A, the limitation fluidic structures 309 are close to one end of the opening 11 of passage, far
From the blind end 12 of passage 300.It is preferred that, the structure of limitation fluid is located at the lower section of the marked region of reagent strip.It is excellent at some
In the mode of choosing, passage has been divided two different parts by the structure of limitation fluid, in the place close to opening, for example, is schemed
Some position of passage is become narrow by 1A, the structure for limiting fluid, meanwhile, the wall of passage is along narrow place to extension
Stretch, so extension is gradual extension, so that, with extension, the cross-sectional area of passage namely gradually increase, so as to allow extension
In one section of passage cross-sectional area be more than second channel cross-sectional area.Or, such as Fig. 1 D and Fig. 1 E, like that, limitation stream
Passage has been divided two different parts by the structure 309 of body, and in the place close to opening, such as Fig. 1 D limit the knot of fluid
Some position of passage is become narrow by structure, then, along stenosis to opening 11 at extend, with extension, passage it is crosscutting
Area does not become larger, and remains unchanged, so as to allow the cross-sectional area of first passage to be consistently less than cutting very much for second channel
Area.Figure 1B is the schematic diagram of passage in the existing detection assembling device of tradition, and whole passage is substantially identical cross section, Huo Zhetong
Distance essence is the same between the wall in road, and passage is in smooth or parallel wall.It is unexpected frightened by experiment of the present utility model
Strange discovery, can be notable using embodiment of the present utility model (such as Figure 1A, Fig. 1 D scheme, or similar scheme)
Reduction mighty torrent phenomenon, can reduce 20-30% probability mighty torrent occur, greatly improve existing product or existing skill
The defect of art, improving tradition, (such as Figure 1B includes reagent strip to detect the performance of analyte in sample using passage.
In some preferred modes, and multiple channel designs of the present utility model (such as Figure 1A, that single of Fig. 1 D
Passage) it can be integrally disposed on plastic plate, such as shown in Fig. 2, such plastic plate includes multiple passages 300, such to carry
Body 100 includes bottom plate 200, multiple notchs 30 is set on bottom plate, neck and bottom plate are bonded to form complete passage 300.
Bottom plate 200 can be transparent plastic bottom board, and notch can be transparent or opaque material.Typically, bottom plate is
Planar transparent, assemble to form complete channel design with neck.In some preferred modes, the closing of the one end of passage 300
312, another end opening 305, transparent bottom plate has one end extended surface 201 at access portal, and the extended surface can pass through transparent
Material be clearly apparent sample in the sample applying zone 503 of reagent strip and apply pad.As shown in Fig. 2 multiple passages are integrated in
On dianegative 200, a fan-shaped card is formed, the card can be easily converted into due to being flexible, or surrounded certain
Shape and be inserted into urine cup and (be discussed in detail below), so as to form complete detection means.Optionally, it is such to insert
Piece can also be used alone, and be directly inserted into liquid sample to be detected.
With reference to specific figure, such plate-inserting structure is described in detail.Such as Fig. 3-5 is shown in Fig. 2
The structural representation of a passage in plate-inserting structure.As shown in figure 3, include a notch 30 (Fig. 4) on bottom plate 200,
Transparent bottom plate and neck combine and just form a complete passage 300, and one end of the passage is closing 312, and the other end is to open
Mouth 305, by opening, extraneous liquid enters passage.Such passage can form the form of substantially cuboid, should
The shape of cuboid is not the cuboid of rule, and two ends are narrow, and middle wide, one end of opening is more wider than other places of passage,
The height of cuboid all essence is the same, in fact, the different structure unit of varying cross-section area is constituted, because height
Equally, then the difference of width is formed the difference of different cross-sectional areas.Fig. 4 is the schematic perspective view of the notch of passage
(being free of egative film 200), in the passage both sides of approximate cuboid, with the symmetrical structure of assistant piece 325,326, assistant piece has flat
Sliding face 321,322, and smooth face Shang You outers side 328,327, each there is certain width plane, such as 1 along side
Millimeter or 2 millimeters, it is smooth, smooth along the face on side, for egative film 200 carry out be bonded together into hermetic seal, so as to allow
Egative film seals up neck, so as to form complete passage 300.From fig. 4, it can be seen that containing one in notch 30 is formed
Neck chamber 329 for arranging detection reagent bar, the neck chamber 329 has certain depth, and the depth is that can be expressed as leading to
The height (in this specific embodiment) in road, while neck has different width, in the place close to opening 305, neck
Two depth wall 332,333 between width (the first width A) be greater than width between two walls 334 and 335 of neck
Spend (the second width B), still, it is wide that the width (the 3rd width C) formed between the wall 331,330 of neck depth is less than second again
Degree.So the intersection of different in width just forms flood control flow structure of the present utility model.Optionally, as shown in figure 4, in fact,
In the local C of the 3rd width, the wall of neck is outside (305 at opening) extension, is gradually to stretch out, with extending out,
Width between two walls is also gradually to increase, and forms " loudspeaker " mouth-shaped one small.Herein, " loudspeaker " mouth of formation is simultaneously
It is a preferred scheme still necessary to the utility model not to be.As shown in fig. 6, such passage does not have " loudspeaker " mouth
Form, but on passage, having becomes narrow 309 at one, stenosis passage caves inward, so as to allow passage here interior
Footpath diminishes, and other places of actual upper channel do not have also forms anti-mighty torrent of the present utility model at actual change, narrow 309
Structure.This inside sunk structure can be unexpected depression or gradual depression, then gradually extend
Go.In the present embodiment (in Fig. 4), the formation of the 3rd width C is the convex shaped of neck wall.Certainly, in order to allow neck shape
Into different width, in addition to neck wall is inwardly projecting, there can also be other manner, for example, set square on the wall for examine groove
Projection, either circular or other any type of projections, so allows neck to have different width.In addition, card is worked as
When being shaped as cylinder or be semicircular of groove, according to same reason, can also set difference between the wall of neck
Projection, so as to form the passage of different in width.
In some preferred modes, tiny groove 312 is formed on the bottom (Fig. 4) of neck, the groove is through whole
The bottom of neck (such as Fig. 3 is exactly the top of passage).So, when having liquid to pour in the opening of passage, tiny is recessed
Groove 312 can exclude gas in time, can faster allow the inside and outside balance for reaching air pressure in sealed passage, can
To improve the time for reaching balance, allow liquid to reply tranquility faster, the influence of mighty torrent can also be reduced.
Further, with reference to rear Fig. 5, shown in 6, the length of whole neck or passage is 57.5 millimeters, detection reagent bar
60 millimeters of length, so allowing a part of reagent strip to be not in neck, but is exposed outside.Second width B of neck is 4.25
Millimeter.3rd width C is 3.25 millimeters, when with it is bell-mouthed when (it cannot also have, for example as shown in Figure 6), the
One width A is 5.4 millimeters (Fig. 5), and the depth of neck is all that identical depth or different parts have different depth.From figure
5, it is apparent that the width from the position of the 3rd width C to the first width A position is gradually increased, this gradually to increase
Plus can also be gentle increase or the violent increase of comparison, this is included in marrow of the present utility model.Preferred
Mode in, the width at opening be gradually increase or passage height be also it is gradually increased, such as it is narrow in Fig. 5
Place 309 be sunken, be then gradually increased width, at 308 position, the height of neck also with wall extension by
It is cumulative to add, a structure 311 for sticking up lip is formed, so relatively open area 310, Ran Houxiang is formed in the place of opening 305
In passage, narrow region is formed, mighty torrent phenomenon so may can be more reduced, impact of the localized liquid to reagent strip is reduced and makees
With.
For example, in another way, such as shown in Fig. 6 (structure for not having mouth), the 3rd width C is 3,25 lis
Rice is less than the second width B (4,25 centimetres), has a segment structure to be 4.25 lis of the second width being consistently less than neck in recess
Rice, then progressivelyes reach the structure of the second width, without bell-mouth structure of air (such as Fig. 5).But, the width of reagent strip is 3.20
Or 3.1 mm in width.So, when reagent strip is arranged in neck, portion of reagent bar is at stenosis 309,
Although reagent strip is not contacted with the wall of neck, for passage, reagent strip is divided into two parts by stenosis, is leaned on
The part and remote narrow part (such as at B) being closely open.When the passage containing reagent strip is (vertically, different in a variety of ways
The inclination of angle) it is inserted into liquid sample or the detection means with passage (such as urine cup) receives liquid sample
(urine), no matter urine is with which kind of angle, in how much entrance urine cups of speed and amount, can significantly reduce mighty torrent phenomenon.
In some preferred modes, the structure of the flood control flow structure is in the lower section of reagent strip marked region, typically,
There is waterline below the marked region of reagent strip, represent that reagent strip is inserted to greatest extent and either immerse or sample floods examination
The position line (such as the 509 of Fig. 1 E) of agent bar, then the structure of anti-mighty torrent is (such as at 331 and 330 in 309, Fig. 4 in Fig. 6
At 900 in structure, or Figure 1A) vicinity of waterline is all located at, for example maintain an equal level with waterline, or on waterline,
But all in the place under marked region.
Examples of implementation of the present utility model are described in detail with reference to specific accompanying drawing.These are specifically implemented
Example is only in the limited list without prejudice to the utility model under spiritual, it is not excluded that those of ordinary skill in the art are existing
There are other specific embodiments that technology and the utility model are combined and produced.
Different detections are carried out in the utility model from a series of DOA products, mainly there is AMP (amphetamine), BAR
(barbital), BZO (Benzodiazepine), COC (cocaine), MET (methamphetamine), MDMA (diformazan methylenedioxymethamphetamine benzene
Amine), MOP (morphine), OPI (morphine), OXY (hydroxyl dihydrocodeinone), MTD (methadone), PCP (Hog), PPX
(propoxyhene), TCA (tricyclic antidepressant), THC (THC), COT (nicotine), BUP (buprenorphine), ACE is (right
Paracetamol), KET (ketamine), MQL (methaqualone), EDDP (2- acetal -1,5 dimethyl -3,3- Diphenyl Pyrroles
Alkane).
The comparative experiments of mighty torrent phenomenon under the different notchs of embodiment 1
The first passage:The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection
Reagent strip 500 is constituted.Passage is formed together with flat carrier including multiple notchs 30 on the carrier 100, neck 30 is length
Square configuration, the setting on neck with different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C of neck
(3.25 millimeters) are less than the second width B (4.25 millimeters), but the second width B is less than the Breadth Maximum A (5.4 of the first width
Millimeter), wherein the first width A is below the marked region of reagent strip and close to opening 305, wherein, the second width B of neck is big
In the width of reagent strip, the first width A it is suitable with the width of reagent strip or essence it is equal (width of reagent strip be 3.2 milli
Rice), the other end of passage is sealing.Detection reagent bar 500 in the detection card, is to be used to detect in sample whether contain Poison
Material, such as hemp, cocaine etc. material, this when, the height or depth of neck is the same.
Second of passage (contrast, traditional notch):Equally, compared with the first passage, difference is:We
With the neck (the distance between corresponding wall in neck is all 4.25 mm wides) of same widths, the width of reagent strip is still
For 3.20 millimeters, position of the reagent strip in neck is as in the first neck.
The third passage:Compared with the first passage, it is other all, unlike:Neck is rectangular shape, in card
There is the setting of different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck is small on groove
In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., have on groove is examined recessed
Fall into position (309), without bell-mouthed structure.Other positions are as the first neck, including reagent strip is also the same
Width.
Operating method:When the detection, it is detection 100 (the detection cards directly containing three of the above channel design) of card are straight
Connect and be inserted into containing in a variety of small molecule drugs mixed solutions.Kept for 1 minute in liquid sample, it is determined that enough samples are used for
Detection.Now, liquid sample can reach marked region 502, and mark along the sample application zone 503 of detection reagent bar 500
Mark substance on region is sufficiently mixed uniformly, detection zone 504 is then reached, in the test results zone of detection zone 504
Domain forms detection symbols, such as detection line, for judging whether contain analyte in liquid sample.Following table is mighty torrent number
Statistical form, such as following table:
As can be seen from the above table, when the structure in neck or when passage occurs with flood control flow structure,
In the neck of all experiments, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without
Fluidic structures are limited in same neck or passage, the incidence of mighty torrent is 15%, and only 86.5% test-strips occur
Control line, illustrates that control line do not occur in the test-strips for also having 13.5%, regardless of detection line, is all represented as invalid knot
Really.Comparatively, the third passage occur mighty torrent probability it is much smaller, compared to the test in traditional existing channel design
Bar.
Comparative experiments (in urine cup) -- the laboratory of mighty torrent phenomenon under the different notchs of embodiment 2
The first neck:The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection
Reagent strip 500 is constituted.Passage is formed together with carrier including multiple notchs 30 on the carrier 100, neck is rectangle shape
Shape, the setting on neck with different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 of neck
Millimeter) be less than the second width B (4.25 millimeters), but the second width B less than the first width A (5.4 millimeters) (bell-mouthed most
Big width), wherein the 3rd width C is below the marked region 502 of reagent strip and close to opening 305, wherein, the second of neck is wide
Spend B be more than reagent strip width, the 3rd width C it is suitable with the width of reagent strip or essence it is equal (width of reagent strip be 3.2
Millimeter), the other end of passage is sealing.Whether the detection reagent bar 500 in the detection card, be used to detect in sample containing poisonous
Product material, such as hemp, cocaine etc. material.
Second of neck (contrast):Equally, compared with the first neck, difference is:We use the card of same widths
Groove (the distance between corresponding wall in neck is all 4.25 mm wides), the width of reagent strip is still 3.20 millimeters, examination
Position of the agent bar in neck is as in the first neck.
The third neck:Compared with the first neck, it is other all, unlike:Neck is rectangular shape, in card
There is the setting of different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck is small on groove
In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., have on groove is examined recessed
Fall into position (309), it is other as the first neck without bell-mouthed structure, including reagent strip is also the same width.
Operating method:When the detection, first the passages of three kinds of different structures (the first neck, second of neck, the
Three kinds are examined groove) card bending (as shown in Figure 2), be inserted into the cup of urine cup, wherein, allow passage opening positioned at urine cup
Bottom (Fig. 8), urine is then quickly poured into urine cup, and (height of cup is 75 millimeters, and the length of whole passage is 57.5
Centimetre, the length of reagent strip is 60 millimeters), whole urine cup (is almost filled with liquid-like in 2 seconds by the volume of urine cup for 50 milliliters
Product).Once being full of urine in urine cup, now, liquid sample can be entered in passage by the opening of passage, along detection reagent
The sample application zone 503 of bar 500, reaches marked region 502, and mark substance on marked region is sufficiently mixed uniformly,
Then detection zone 504 is reached, detection symbols, such as detection line, for judging are formed in the testing result region of detection zone 504
Whether contain analyte in liquid sample.Following table is the statistical form of mighty torrent number, such as following table:
As can be seen from the above table, when the structure with flood control flow structure occurs in neck, in all examinations
In the urine cup tested, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without limiting fluid
Structure is in same neck or passage, and the incidence of mighty torrent is 17%, and control line, explanation occur in only 83% test-strips
There is not control line in also 17% test-strips, regardless of detection line, are all represented as invalid result.Comparatively,
The probability that mighty torrent occur in three kinds of passages is much smaller, compared to the test-strips in traditional existing channel design.
The comparative experiments (in urine cup) of mighty torrent phenomenon under the different notchs of embodiment 3 -- it is clinical
The first neck:The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection
Reagent strip 500 is constituted.Passage is formed together with carrier including multiple notchs 30 on the carrier 100, neck is rectangle shape
Shape, the setting on neck with different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 of neck
Millimeter) be less than the second width B (4.25 millimeters), but the second width B less than the first width A (5.4 millimeters) (bell-mouthed most
Big width), wherein the 3rd width C is below the marked region 502 of reagent strip and close to opening 305, wherein, the second of neck is wide
Spend B be more than reagent strip width, the 3rd width C it is suitable with the width of reagent strip or essence it is equal (width of reagent strip be 3.2
Millimeter), the other end of passage is sealing.Whether the detection reagent bar 500 in the detection card, be used to detect in sample containing poisonous
Product material, such as hemp, cocaine etc. material.
Second of neck (contrast):Equally, compared with the first neck, difference is:We use the card of same widths
Groove (the distance between corresponding wall in neck is all 4.25 mm wides), the width of reagent strip is still 3.20 millimeters, examination
Position of the agent bar in neck is as in the first neck.
The third neck:Compared with the first neck, it is other all, unlike:Neck is rectangular shape, in card
There is the setting of different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck is small on groove
In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., have on groove is examined recessed
Fall into position (309), it is other as the first neck without bell-mouthed structure, including reagent strip is also the same width.
Operating method:Before detection is carried out, first the passages of three kinds of different structures (the first neck, second of neck, the
Three kinds are examined groove) card it is (as shown in Figure 2) bending, be inserted into the cup of urine cup (such as Fig. 8), wherein, allow passage opening position
In the bottom of urine cup, then carry out different people and carry out clinical trial, the people for exactly carrying out no professional operation is collected urine,
Typically directly genitals are allowed to be urinated into urine cup into urine, so that the probability of mighty torrent phenomenon generation is observed, as a result such as following table:
As can be seen from the above table, when the structure with flood control flow structure occurs in neck, in all examinations
In the urine cup tested, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without limiting fluid
Structure is in same neck or passage, and the incidence of mighty torrent is 20%, and control line, explanation occur in only 80% test-strips
There is not control line in also 20% test-strips, regardless of detection line, are all represented as invalid result.Comparatively,
The probability that mighty torrent occur in three kinds of passages is much smaller, compared to the test-strips in traditional existing channel design.
Examples of implementation 4:The experiment of mighty torrent phenomenon under extreme condition
The first neck:The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection
Reagent strip 500 is constituted.Passage is formed together with carrier including multiple notchs 30 on the carrier 100, neck is rectangle shape
Shape, the setting on neck with different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 of neck
Millimeter) be less than the second width B (4.25 millimeters), but the second width B less than the first width A (5.4 millimeters) (bell-mouthed most
Big width), wherein the 3rd width C is below the marked region 502 of reagent strip and close to opening 305, wherein, the second of neck is wide
Spend B be more than reagent strip width, the 3rd width C it is suitable with the width of reagent strip or essence it is equal (width of reagent strip be 3.2
Millimeter), the other end of passage is sealing.Whether the detection reagent bar 500 in the detection card, be used to detect in sample containing poisonous
Product material, such as hemp, cocaine etc. material.
Second of neck (contrast):Equally, compared with the first neck, difference is:We use the card of same widths
Groove (the distance between corresponding wall in neck is all 4.25 mm wides), the width of reagent strip is still 3.20 millimeters, examination
Position of the agent bar in neck is as in the first neck.
The third neck:Compared with the first neck, it is other all, unlike:Neck is rectangular shape, in card
There is the setting of different in width, such as schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck is small on groove
In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., have on groove is examined recessed
Fall into position (309), it is other as the first neck without bell-mouthed structure, including reagent strip is also the same width.
Certain urine is poured into cavity 90 in urine cup (Fig. 8), is formulated once being circulated into after scale, is then put into and shakes
What progress was violent in bed rocks (frequency is that hz is per second), and the time rocked is 5 minutes or so, then removes, sees the result of experiment
How, it is as a result as follows:
Under conditions of extremely acutely, mighty torrent phenomenon occurs for the structure only one of which of the first neck or passage, still
Control line still occurs, on the contrary, traditional channel design, because the structure for flow structure of not controlling flood, only 86.7% reagent
There is control line in bar, and some does not occur, and illustrates influence due to mighty torrent and as invalid detection.Meanwhile, the third
It is more superior than traditional.
Claims (10)
1. a kind of device for detecting analyte in sample, it is characterised in that the device includes one end open, and it is logical that one end is closed
Road, the wherein passage are used to set detection reagent bar, wherein, passage includes flood control flow structure, and the structure allows the cross section of passage
Product diminishes at flood control flow structure.
2. the device of analyte in detection sample according to claim 1, it is characterised in that passage includes bottom plate and card
Slot structure, wherein bottom plate and notch formation one end open, the sealed described passage in one end, wherein, opening is located at detection
Near the sample applying zone of reagent strip.
3. the device of analyte in detection sample according to claim 1, it is characterised in that the mark on detection reagent bar
Remember that region is located in passage on flood control flow structure.
4. the device of analyte in detection sample according to claim 1, it is characterised in that bottom plate is transparent, is put down
Face, notch is nontransparent.
5. the device of analyte in detection sample according to claim 1, it is characterised in that the structure handle of limitation fluid
Passage has divided two different parts, and a part is to control flood flow structure to the first paragraph passage of access portal, and another part is just
It is to control flood flow structure to one end second segment passage of closed channel, in the place close to opening, limits the structure of fluid passage
Some position become it is narrow, meanwhile, the wall of passage stretches out along narrow place, so extension be gradually extend,
So as to extension, the cross-sectional area of passage namely gradually increase, so that the cross-sectional area of one end passage allowed in extension
More than the cross-sectional area of second channel.
6. the device of analyte in the detection sample according to one of claim 1-5, it is characterised in that described passage
Include detection reagent bar, wherein the structure of limitation fluid is located under the marked region of reagent strip.
7. the device of analyte in the detection sample according to one of claim 1-5, it is characterised in that described device
Also include the cavity of a receiving liquid sample, wherein, the passage is located in the cavity, wherein, the opening position of described passage
Near the bottom of cavity.
8. the device of analyte in detection sample according to claim 2, it is characterised in that described neck includes length
The neck of cube, the neck is divided into two parts, the Part I with neck opening and the Part II with blind end,
Wherein, the width of Part I and Part II interface diminishes, and forms the first width.
9. the device of analyte in detection sample according to claim 8, it is characterised in that second is formed at opening wide
Degree, with blind end the 3rd width of formation, wherein the first width is less than the 3rd width.
10. the device of analyte in detection sample according to claim 9, it is characterised in that the 3rd section is less than second
Width.
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