CN107664702A

CN107664702A - The device of analyte in a kind of detection sample

Info

Publication number: CN107664702A
Application number: CN201610613281.7A
Authority: CN
Inventors: 洪亮
Original assignee: Hangzhou Biotest Biotech Co Ltd
Current assignee: Hangzhou Biotest Biotech Co Ltd
Priority date: 2016-07-27
Filing date: 2016-07-27
Publication date: 2018-02-06

Abstract

The present invention provides a kind of device for detecting analyte in fluid sample, and the device includes one end open, the passage of one end closing, wherein the passage is used to set detection reagent bar, wherein, passage includes flood control flow structure, and the structure allows the cross-sectional area of passage to diminish at flood control flow structure.By device, the mighty torrent phenomenon of test agent bar can be prevented, reduce error rate.

Description

The device of analyte in a kind of detection sample

Technical field

The present invention relates to a kind of carrier of containing detection reagent strip, detection reagent bar especially in quick diagnosis field Storing apparatus.

Background technology

Be presently used for detect sample in whether the detection means containing analyte, be largely used to hospital or family In, these detection means for being applied to quick diagnosis include one or more detection reagent bars, such as early pregnancy detection, drug abuse Detection etc..The detection means of this quick diagnosis is very convenient, can be at one minute, or at most ten minutes or so are detecting Testing result is obtained on reagent strip.

In these detection means, detection reagent bar is normally on certain carrier.Carrier can be the mould of plate shape Or plate etc..Carrier is typically made up of neck or passage, and a neck or passage can accommodate a detection reagent bar, So the carrier with detection reagent bar can be used alone, and can also be used cooperatively with container.When the carrier as come When containing detection reagent strip, can be used directly to detect in sample whether contain material interested, this when, sample Can be liquid sample, such as urine.It is inserted into when the carrier with detection reagent bar in sample, or allows liquid sample stream When entering into the container with carrier, because the flowing that liquid enters in carrier is not smooth flow, but mixed and disorderly nothing Chapter, usually pour in a short period of time neck either in passage or containing detection reagent strip intracavitary, so, Commonly referred to as (flooding) " mighty torrent " phenomenon can be caused, so as to influence the normal work of detection reagent bar and normal reaction.

It is necessary to be improved to the carrier of traditional carrying detection reagent bar, so as to avoid such as mighty torrent phenomenon, So as to improve the accuracy of detection reagent bar and validity.

The content of the invention

In order to solve problems of the prior art, the present invention provides such a carrier, and the carrier includes being used to hold Receive the passage of detection reagent bar, passage end closing, the other end has opening, and detected fluid sample can be opened by this Mouth is entered in passage, wherein, flood control flow structure is included in the passage.Or the present invention provides a kind of passage, the passage should Passage end is closed, and the other end has opening, and detected fluid sample can be entered in passage by the opening, wherein, Include the structure of anti-mighty torrent in the passage.Preferably, contacted into the liquid of passage with detection reagent bar, it is preferred that with detection The sample application zone contact of reagent strip, or, simply the sample application zone with detection reagent bar contacts.

In some preferable modes, the flood control flow structure is located near the opening of passage.

In some preferable modes, flood control flow structure is located under the marked region of detection reagent bar or away from detection Reagent strip marked region.In some preferable modes, flood control flow structure is located near the fluid applying zone of detection reagent bar Or the marked region with detection reagent bar is not in the same horizontal position.Preferably, flood control flow structure is located at reagent strip Near waterline, it is furthermore preferred that flood control flow structure is located on the waterline of reagent strip, under marked region.

In some preferable modes, flood control flow structure is included in protruding component in passage, and the protruding component allows part The cross-sectional area of passage is reduced.In some preferable modes, protruding component is respectively on the two side of cavity, and to passage Protrusions.Preferably, flood control flow structure allows part to allow the cross-sectional area of passage portion to reduce, or allows passage portion to become narrow.

In some preferable modes, the opening cross-sectional area of passage is more than containing the crosscutting of the passage at flood control flow structure Area.In some preferable modes, the width of the opening of passage is more than the width of channel interior.

In some preferable modes, passage is divided into two parts by flood control flow structure, and a part is that flood control flow structure arrives The first paragraph passage of access portal, another part are exactly to control flood flow structure to one end second segment passage of closed channel, wherein, the The cross-sectional area of one section of passage is more than the cross-sectional area of second segment passage；Or the cross section of the passage portion of first paragraph passage Passage portion cross-sectional area of the product more than second segment passage；Or the cross-sectional area of each part of first paragraph passage is both greater than The cross-sectional area of each part of second segment passage.The intersection of first paragraph passage and the second end passage forms the flood control of the present invention Flow structure, or the intersection of first paragraph passage and the second end passage become narrow by passage.Preferably, first paragraph passage and second The intersection of end passage gradually becomes narrow region for one, allows passage to become narrower at the region.Form the anti-of the present invention Mighty torrent structure, when passage is cylindrical, the size of passage can be weighed with the cross-sectional area of passage.When passage is length When square or cuboid, in the case of highly consistent, the bigger place of the width of passage, overall cross-sectional area is got over Greatly, so, it can also be that the width of first paragraph passage is more than the width of second segment passage, wherein, first passage and second channel Height it is the same.

Preferably, it is more than the width of channel restriction, but the height of the opening in passage in the width of the opening of passage Spend the height also greater than channel restriction.

In some preferable modes, passage includes bottom plate and notch, and its bottom plate and notch form one end Opening, the passage of one end sealing, wherein, opening is near the sample applying zone of test-strips.Preferably, neck includes narrow Neck is divided into two parts by structure, the narrow structures, and the width wherein at narrow structures is less than the width at the other positions of neck. Preferably, an elongated sulculus is included at the top of neck, the sulculus runs through whole passage, or through whole neck Top.

In some preferable modes, detection reagent bar can also be included in cavity lane, the sample of the reagent strip applies Region is located near access portal, sample absorbance region be located at channel enclosed one end or near.In some preferable sides In formula, the marked region on reagent strip is located in passage near flow structure of controlling flood.

In foregoing all embodiments, it is transparent flat that the passage for containing detection reagent strip on carrier includes one Harden structure and notch, wherein, described notch and slab construction form described passage.Preferably, notch To be nontransparent.In some preferable modes, the detection zone of detection reagent bar, Huo Zhejian can be seen through slab construction The detection zone of test agent bar face transparent plate structure or with the facing setting of transparent plate structure.

On the other hand, the present invention provides a kind of detection means, and the wherein device includes foregoing described carrier or logical Road, the carrier include the passage for containing detection reagent strip, and passage end closing, the other end has opening, detected Fluid sample can be entered in passage by the opening, wherein, flood control flow structure is included in the passage.In some preferable sides In formula, the device also includes detection reagent bar, wherein, the reagent strip is located in described passage.Foregoing all flood control flow structures Mode may be included within the present invention detection means in.Preferably, flood control flow structure is located at the mark on detection reagent bar Remember under region, or, flood control flow structure is located between the marked region on detection reagent bar and sample areas；Or reagent Marked region on bar is on flood control flow structure.

In a preferable mode, described device also includes the cavity of a receiving liquid sample, wherein, the carrier In the cavity, wherein, the sample applying zone of described inspection reagent strip is close to the bottom of cavity, so, the liquid of cavity Body sample can just be entered by the opening of passage in passage come, contacted with the sample applied area of the reagent strip in passage, so as to Liquid is with reagent strip from sample applied area sequentially through mark zone, detection zone, detection control area (if there is), most Zhongdao Up to suction zones (if there is).

The present invention provides a kind of method for detecting fluid sample analyte, and this method includes：A kind of detection dress is provided Put, the detection means includes carrier, and the carrier includes the passage for containing detection reagent strip, and passage end closing is another End has opening, and detected fluid sample can be entered in passage by the opening, wherein, include anti-mighty torrent in the passage Structure, wherein, the passage includes detection reagent bar, wherein, flood control flow structure is located at the marked region of detection reagent bar and led to Between road opening；Allow fluid sample to enter by the opening of passage in passage, allow the sample applying zone of detection reagent bar to contact Fluid sample in passage, so as to examine the analyte in fluid sample.Or flood control flow structure is located at detection reagent bar Under marked region, on access portal.

In some preferable modes, flood control flow structure is located under the marked region of detection reagent bar or away from detection Reagent strip marked region.In some preferable modes, flood control flow structure is located near the fluid applying zone of detection reagent bar Or the marked region with detection reagent bar is not in the same horizontal position.

In some preferable modes, flood control flow structure is included in the protruding component in passage, and the protruding component is by passage Cross-sectional area reduce.In some preferable modes, protruding component is respectively on the two side of cavity, and to passage convexity Rise.

In some preferable modes, passage is divided into two parts by flood control flow structure, and a part is that flood control flow structure arrives The first paragraph passage of access portal, another part are exactly to control flood flow structure to one end second segment passage of closed channel, wherein, the The cross-sectional area of one section of passage is more than the cross-sectional area of second segment passage.When passage is cylindrical, passage can be used Cross-sectional area weighs the size of passage.When passage is rectangle or cuboid, in the case of highly consistent, passage The bigger place of width, overall cross-sectional area is bigger, so, can also be that the width of first paragraph passage is more than second segment The width of passage, wherein, first passage is as the height of second channel.

Brief description of the drawings

Fig. 1 is the principle schematic of the present invention and the figure compared with the structure of conventional channels.Wherein, Figure 1A and Fig. 1 D are this hair The principle schematic of bright carrier, Figure 1B are the structural representation of traditional carrying detection reagent bar, and Fig. 1 C are detection reagent bar Structural representation；Fig. 1 E are the combining structure schematic diagram of carrier structure and reagent strip in one embodiment of the present invention.

Fig. 2 is that multiple passages are arranged on the carrier of one embodiment of the invention, can be with containing detection reagent strip in the passage Dimensional structure diagram.

Fig. 3 is the amplification dimensional structure diagram of a channel design in Fig. 2；

Fig. 4 is the schematic perspective view for the notch for removing transparent plate structure；

Fig. 5 is the partial enlargement structural representation of a passage；

Fig. 6 A are the profilographs of a passage of an alternative embodiment of the invention, and the dress with Fig. 6 B for reagent strip With position view；

Fig. 7 is the principle schematic that the liquid flowing containing reagent strip causes mighty torrent phenomenon in conventional channels.

Fig. 8 is the assembly structure diagram of the urine cup containing passage of the present invention in an embodiment of the invention.

Embodiment

Structure of the present invention or these used technical terms are described further below.

Detection

Detection represents to chemically examine or test a kind of material or material whether there is, such as, but this is not limited to, chemical substance, The generation of organic compound, inorganic compound, metabolism product, medicine or drug metabolite, organic organization or organic organization Thank thing, nucleic acid, protein or polymer.In addition, detection represents test substances or the quantity of material.Furtherly, table is gone back in chemical examination Show immune detection, chemical detection, enzyme detection etc..

Sample

Signified sample of the invention refers to those and can be used for detecting, chemically examine or diagnosing whether there is analyte interested The material of matter.Sample can be, for example, fluid sample, it is liquid sample that fluid sample, which can be, and liquid sample can include blood Liquid, blood plasma, serum, urine, saliva and various juices, solid sample and semi-solid samples can also be included by anticipating The liquid solution formed afterwards.The methods of sample come can be used for immune detection, chemical detection, enzyme detection is collected to detect whether Analyte be present.

Detection reagent bar

The horizontal effluent reagent strip 500 that the detection reagent bar of the present invention can be known as is applied to, these detection examinations The concrete structure and Cleaning Principle of agent bar are technology known to persons skilled in the art in the prior art.Common detection Reagent strip 500, including sample collecting zone 503, marked region 502, detection zone 504 and suction zone 501, sample collecting region Domain includes sample receiving pad, and marked region 502 includes label pad, and suction zone 501 can include adsorptive pads, wherein detection zone Include that the necessary chemical substance containing analyte, such as immunoreagent or enzymology reagent can be detected whether on 504.One As commonly use detection reagent bar be nitrocellulose assay strip, i.e. detection zone 504 includes nitrocellulose filter, nitric acid fibre Tie up and specific binding molecule is fixed on plain film to show the result of detection；Cellulose acetate film or nylon membrane etc. are can also be, when So, testing result control area 505 can also be included in the downstream of detection zone, generally, on control area and detection zone with The form of horizontal line occurs, and is detection line or control line.Such detection reagent bar is traditional reagent strip, certainly or Other other types of reagent strips detected using capillarity.In addition, carry dry chemistry test on general detection reagent bar Agent composition, such as fixed antibody or other reagents, after liquid is run into, liquid is as capillarity is along reagent strip stream It is dynamic, with flowing, allow dry agent formulations to be dissolved in liquid, so as to which the dry reagent to next regional processing in the area occurs instead Should, so as to carry out necessary detection.Liquid flowing is mainly carried out by capillarity.

Analyte

Some haptens materials can be included with the example for the analyte being related in the present invention, these haptens include poison Product (such as drug abuse)." drug abuse " (DOA) refers to that non-medical destination uses medicine (generally playing paralysis nerve). Abusing these medicines can cause body ＆ mind to suffer damage, and produce dependence, addicted and/or dead.Drug abuse Example includes cocaine；Amphetamine AMP is (for example, black beauty, white amphetamine tablet, dextroamphetamine, dextrorotation benzene are different Propylamine tablet, Beans)；Crystal methamphetamine MET (crank, meth, crystal, speed)；Barbiturate BAR (such as Valium, Roche Pharmaceuticals, Nutley, New Jersey)；Sedative (paramedicines of sleeping)；Wheat Angle acid acyl diethylamine (LSD)；Inhibitor (downers, goofballs, barbs, blue devils, yellow jackets, Methaqualone)；The anti-antidepressant of tricyclic antidepressants (TCA, i.e. imipramine, amitriptyline and doxepin)；Diformazan methylenedioxymethamphetamine aniline MDMA；Hog (PCP)；Tetrahydrocannabinol (THC, pot, dope, hash, weed, etc..)；Opiate (i.e. morphine MOP or, opium, cocaine COC；, heroin, hydroxyl dihydrocodeinone)；Anxiolytic and hypnotic sedative agent, anxiolytic It is that one kind is mainly used in anxiety reduction, anxiety, fear, sets the mind at rest, has the medicine of hypnosis sedation, including Benzodiazepine concurrently Class BZO (benzodiazepines), atypia BZ classes, fusion phenodiazine NB23C classes, benzene nitrogen Zhuo class, the part class of BZ acceptors, open Ring BZ classes, diphenylmethane derivatives, piperazine carboxylic acid salt, piperidine carboxylic acid salt, Kui oxazoline ketone, thiazine and thiazole, Other heterocyclics, imidazole type calmness/anodyne (such as hydroxyl dihydrocodeinone OXY, methadone MTD), propanediol derivative-ammonia Formate ester, aliphatic compound, anthracene derivative etc..Detection, which is can be used for, using the detection means of the present invention belongs to medical science The detection of purposes but easy overdose, such as tricyclic antidepressant (imipramine or the like) and Paracetamol.This A little medicines can resolve into different small-molecule substances after being absorbed by the body, and these small-molecule substances are present in blood, urine, saliva In the body fluid such as liquid, sweat or there is above-mentioned small-molecule substance in part body fluid.

Detection means

Detection means refer to for detect in sample whether the device containing analyte.In the present invention, detection dress The detection means especially represented in quick diagnosis field is put, such as detection reagent bar 500, detection card or plate-inserting structure 100, The various forms of detection means such as test bar, detection cup (shown in Fig. 8).Herein, reagent strip is inserted into the (figure of detection card 100 2) in passage 300, wherein, make the sample applied area of test-strips exposed (in such as Fig. 5 and Fig. 7) from opening 305, thus formed Detection card, detection card inherently can be detected with complete independently.Such as Figure 1A, with one of passage come illustrate detection original Reason, when in use, detection card is inserted into liquid sample, and because the passage end 12 of detection card is closed, the other end is opened Mouth 11, such liquid sample is flowed into passage with regard to some, but because passage end is closed, the inflow of liquid is in passage Be internally formed closed gas, so as under external and internal pressure, in liquid inlet channel after can only keep certain water in the channel Flat height, as during the entering in passage and be maintained at fixed level height of liquid, liquid just submerges the sample of reagent strip This applying zone, liquid are flowed up, so as to complete to detect, if reagent due to the capillarity of reagent strip along reagent strip There are lines or occurs without lines in the detection zone of bar, means that and whether there is analyte in sample.Figure 1B is tradition The passage detected using the principle and reagent strip installation diagram.

No matter the single reagent strip as Figure 1B carries out the detection of analyte in one or several kinds of liquid samples, also It is as Fig. 2, multiple detection test-strips are disposed in multiple different passages 300 (the basic phase of structure of each passage Together), each detection reagent bar corresponds to a kind of analyte, and so detection card 100 is directly inserted into liquid sample, can be with Once carry out the detection of multiple different analytes, such as 3,10,15 different analytes.It is, of course, also possible to such as Fig. 8 is such, the passage containing reagent strip or the cards of multiple passages is disposed in such as the multiple reagent strips of Fig. 2 is disposed in In Fig. 8 container (such as generally as described in urine cup in), allow one end of access portal to be located at the bottom 92 of urine cup, channel enclosed One end 312 be located near urine cup mouth 91, so, when urine cup collects urine, as urine enters urine cup In cavity 90, single channels operation principle as previously described is the same, and each passage is due to the effect of closed gas, each logical There is the liquid (when inside and outside air pressure balance) of certain altitude in road, so as to complete the reaction of each reagent strip.

Detection means can block 20 matching used parts including detection card 20 and with detection, as shown in figure 8, urine cup 2 Can be as a kind of form of detection means.

In current detection, this cassette or cup type detection means, it is required for detection reagent bar 500 with one Carrier 100 supports, and the function of competence exertion detection, so this carrier can include multiple passages 300, each logical Road 300 is inner can to include a reagent strip 500, and each reagent strip corresponds to one or several kinds of analytes.Or at least A passage is needed, reagent strip is set in the passage.However, in existing traditional field, using passage principle (closed gas with And external and internal pressure) under detected, because reagent strip is located in passage, because cooperation or thing between reagent strip and passage Position is managed, often result in some many defects of reagent strip, a kind of defect such as patent, the patent customer service phenomenon of broken string. Because the wall of detection reagent bar and passage usually contacts closely, so as to produce capillarity, so as to cause to break.Solve Method is just to try to not allow detection reagent bar to be contacted with the wall of passage or bump contact, i.e., such as Figure 1B or Fig. 7 that Sample, allow passage and detection reagent bar that there is a certain distance, such as 1-4 millimeters either farther distance or several millimeters Distance.Although so can with it is relatively effective solve the problems, such as broken string, therewith with the problem of, and it is maximum the problem of just It is " mighty torrent " problem.Detected because no matter being directly inserted into the reagent strip in single passage in liquid sample, or inspection Surveying device (such as urine cup) includes multiple passages to carry out the detection of analyte in liquid sample, can all there is mighty torrent phenomenon Appearance.

So-called mighty torrent phenomenon, it is exactly within the of short duration time, substantial amounts of liquid-like 701 is gushed with reference to Fig. 7 further instructions Enter onto reagent strip, allowing reagent strip 500 to have little time to carry out normally flowing by capillarity absorption sample, (561 liquid is By the vestige of the flowing of normal capillarity), but more sample is rushed in test-strips or on surface (indicated by 560 Liquid), by the quick flow forward in the surface of test-strips, due to most liquid-like flowing this be not capillarity stream Dynamic, but directly quickly flowed on surface, it is being that dry chemistry reagent on reagent strip is difficult quick in a short time so to handle It is completely dissolved and is discharged into liquid sample, so, it is not in lines to have resulted in detection zone or detection control area. Because the liquid on surface without or it is less flowed faster by capillarity, moistened other portions of reagent strip in advance Point, therefore, allow reagent strip to lose main capillarity in advance, so, can hardly just allow and carry or dissolved with reagent The reagent of dry-cure flows further along on bar, so, is wet although test strips look, but without generation essence Reaction, so as to be reacted in detection zone or control zone, testing result inaccuracy is also resulted in, will not sometimes have been gone out The situation of existing testing result.

As shown in Figure 7, in traditional device, reagent strip 500 is typically not directly contacted with that (most passages carry with passage 300 Support frame), such reagent strip and passage are once inserted into liquid sample 700, because different pairs of operating personnel are different, are inserted Depth, the speed of insertion entered, and different (the static liquid flowers for either whether flowing or splashing of the state 701 of liquid sample 701), when the passage containing test-strips enters in liquid sample, due to the influence of microenvironment in short-term, substantial amounts of liquid can be caused to exist Poured in the very short time in passage, under microenvironment, liquid is poured in passage by access portal 305, due to logical The one end 312 in road 300 seals, and the liquid of entrance just seals one section of gas with the end, so as to produce pressure to the liquid level of liquid, Entered so as to limit more liquid in passage, be finally reached balance, allow liquid to be in certain height in passage.Although It is very short to reach the time of balance, but in this process, liquid is in rambling and right in the state of microcosmic Reagent strip produces many different directions, very big impulsive force, so as to cause so-called mighty torrent phenomenon.Same similar reason, Passage containing reagent strip or card 100 are arranged in urine cup by static, when being injected into urine cup in liquid sample, such as are urinated Liquid, it is although of short duration so as to reach the process of internal and external equilibrium subsequently into passage because liquid quickly flows in a short time, But the liquid flowing under microenvironment is still fierce, so as to there is partially liq directly from reagent strip surface flow, so as to make Into mighty torrent phenomenon, so as to make testing result failure or test result inaccurate.Certainly, also have, because pouring into liquid into urine cup The direction of body sample, angle, inject the difference of the speed of liquid；Further, especially, when being detected, subject is straight Genitals to be connected to inject in urine cup urine, urine cup usually contains the reagent strip with passage, in this case, due to Different subjects does not have the operational capacity of specialty, urinates the angle into the urine to urine cup, and size is how many to wait also phase not to the utmost Together, especially, it is in feeder connection in relative complex environment, allows test in a word under conditions of one is extremely complex, So, it is easier to mighty torrent phenomenon is caused, so as to influence final testing result.

In order to overcome this defect, carrier 100 or passage 300 as present invention use, liquid sample can be limited Arbitrarily flowed in passage, so as to reduce the generation of mighty torrent.Further, the structure of the flood control flow structure can quickly allow also liquid Body is in tranquility, either quickly reach internal and external equilibrium or, it is possible to reduce liquid does not pass through the capillarity stream of test-strips Dynamic phenomenon.As analysis above, it is capillarity that reagent strip flows main effect by liquid, and caused by mighty torrent be exactly Liquid is allowed to f hardly pass through or seldom be flowed by capillarity on reagent strip.

Carrier or passage

Either passage 300 refers to part or element for containing detection reagent strip 500 to carrier 100.Such as Figure 1A and Fig. 7 Shown, detection reagent bar 500 can be arranged in the passage 300 on carrier, one end closing 312 of the passage, another end opening 305, extraneous liquid is entered in passage by opening, when carrier 100 is uprightly placed or is horizontal positioned, detection examination Agent bar 500 all typically drops out all without from carrier 100, and concretely, reagent strip will not take off from the passage 300 on carrier Drop out and.Herein, carrier inherently passage, or a kind of form of passage inherently carrier, passage is interior to accommodate detection Reagent strip.According to this meaning, the present invention provides a kind of passage, passage end closing, another end opening, detection reagent bar It is disposed in passage.Preferably, the sample applying zone 503 of detection reagent bar detects control area close to the opening of passage Positioned at the blind end close to passage.The element of carrier 100 is formed, can be the form of neck or the form of passage 300, or, neck and a slab construction assemble the passage 300 to form the present invention.The number of neck or passage 300, Ke Yishi 1,2,3,4 even more more.In general, a neck or passage 300 can accommodate a detection reagent bar 500.Typically, clamp structure, such structure are included in one end of one end of passage, such as closing, passage 300 or neck Reagent strip can be clamped.Certain clamp structure it is not necessary to structure, it is only a preferable technical scheme.

In some preferable modes, the structure for flow structure of controlling flood is located in passage, and passage is divided into two not by the structure With part, such as be divided into first passage and second channel, first passage be located at the opening of passage to flow structure of controlling flood, second Blind end of the passage from the position of flood control flow structure to passage, first passage and second channel intersection are flow structure of controlling flood. The structure of the limitation fluid reduces the cross-sectional area of passage or makes limitation fluidic structures that one part of passage formation is narrow and small Space.In some specific modes, such as Figure 1A, the limitation fluidic structures 309 are close to one end of the opening 11 of passage, far From the blind end 12 of passage 300.Preferably, limit fluid structure be located at reagent strip marked region lower section.It is excellent at some In the mode of choosing, passage has been divided two different parts by the structure for limiting fluid, in the place of close opening, such as is schemed 1A, limit the structure of fluid and some position of passage is become narrow, meanwhile, the wall of passage is along narrow place to extension Stretch, so extension is gradually to extend, so as to, with extension, the cross-sectional area of passage namely gradually increase, so as to allow extension In one section of passage cross-sectional area be more than second channel cross-sectional area.Or such as Fig. 1 D and Fig. 1 E, like that, limitation stream Passage has been divided two different parts by the structure 309 of body, close to the place of opening, such as Fig. 1 D, limits the knot of fluid Some position of passage is become narrow by structure, then, along stenosis to opening 11 at extend, with extension, passage it is crosscutting Area does not become larger, and remains unchanged, so as to allow the cross-sectional area of first passage to be consistently less than cutting very much for second channel Area.Figure 1B is the schematic diagram of passage in the existing detection assembling device of tradition, and whole passage is substantially identical cross section, Huo Zhetong Distance essence is the same between the wall in road, and passage is in smooth or parallel wall.It is unexpected surprised by the experiment of the present invention It was found that flood can significantly be reduced using embodiment of the present invention (such as Figure 1A, Fig. 1 D scheme, or similar scheme) Flow phenomenon, the mighty torrent that can reduce 20-30% probability occur, and greatly improve lacking for existing product or prior art Fall into, improving tradition, (such as Figure 1B 0 includes reagent strip to detect the performance of analyte in sample using passage.

In some preferable modes, multiple channel designs of the invention (such as Figure 1A, Fig. 1 D single leading to of that Road) it can be integrally disposed on plastic plate, such as shown in Fig. 2, such plastic plate includes multiple passages 300, such carrier 100 include bottom plate 200, and multiple notchs 30 are set on bottom plate, and neck and bottom plate are bonded to form complete passage 300.Bottom Plate 200 can be transparent plastic bottom board, and notch can be transparent or opaque material.Typically, bottom plate is flat Face is transparent, assembles to form complete channel design with neck.In some preferable modes, the one end of passage 300 closing 312, Another end opening 305, transparent bottom plate have one end extended surface 201 at access portal, and the extended surface can pass through transparent material The sample that matter is clearly apparent in the sample applying zone 503 of reagent strip applies pad.As shown in Fig. 2 multiple passages be integrated in it is transparent On bottom plate 200, a fan-shaped card is formed, the card can be easily converted into, or surround certain shape due to being flexible Shape is simultaneously inserted into urine cup and (is discussed in detail below), so as to form complete detection means.Optionally, such inserted sheet It can be used alone, be directly inserted into liquid sample to be detected.

With reference to specific figure, such plate-inserting structure is described in detail.Such as Fig. 3-5 is shown in Fig. 2 The structural representation of a passage in plate-inserting structure.As shown in figure 3, include a notch 30 (Fig. 4) on bottom plate 200, Transparent bottom plate and neck combine and just form a complete passage 300, and one end of the passage is closing 312, and the other end is to open Mouth 305, by opening, extraneous liquid enters passage.Such passage can form the form of substantially cuboid, should The shape of cuboid is not the cuboid of rule, and both ends are narrow, and middle wide, one end of opening is more wider than other places of passage, The height of cuboid all essence is the same, in fact, the different structure unit of varying cross-section area is constituted, because height Equally, then the difference of width is formed the difference of different cross-sectional areas.Fig. 4 is the schematic perspective view of the notch of passage (being free of egative film 200), in the passage both sides of approximate cuboid, there is the symmetrical structure of assistant piece 325,326, assistant piece has flat Sliding face 321,322, and smooth face Shang You outers side 328,327, each there is certain width plane, such as 1 along side Millimeter or 2 millimeters, it is smooth, smooth along the face on side, for carrying out being bonded together into hermetic seal with egative film 200, so as to allow Egative film seals up neck, so as to form complete passage 300.From fig. 4, it can be seen that contain one in notch 30 is formed For arranging the neck chamber 329 of detection reagent bar, the neck chamber 329 has certain depth, and the depth can be expressed as leading to The height (in this specific embodiment) in road, while neck has different width, close to the place of opening 305, neck Two depth wall 332,333 between width (the first width A) be greater than width between two walls 334 and 335 of neck Spend (the second width B), still, it is wide that the width (the 3rd width C) formed between the wall 331,330 of neck depth is less than second again Degree.So the intersection of different in width just forms the flood control flow structure of the present invention.Optionally, as shown in figure 4, in fact, The local C of three width, the wall of neck are outside (opening 305) extensions, are gradually to stretch out, with extending out, two Width between wall is also gradually to increase, and forms small " loudspeaker " mouth-shaped.Herein, " loudspeaker " mouth of formation is not It is a preferable scheme still necessary to the present invention.As shown in fig. 6, such passage does not have the form of " loudspeaker " mouth, But on passage, having becomes narrow 309 at one, stenosis passage caves inward, so as to allow the internal diameter of passage here to diminish, Other places of actual upper channel do not have the actual structure for changing, the anti-mighty torrent of the present invention also being formed at narrow 309.It is this to Interior sunk structure can be unexpected depression or gradual depression, then gradually extend out.In this implementation In mode (in Fig. 4), the formation of the 3rd width C is the convex shaped of neck wall.Certainly, in order to allow neck to form different width Degree, in addition to neck wall is inwardly projecting, there can also be other manner, such as square projection is set on the wall for examine groove, or Circular, or other any type of projections, so allow neck that there is different width.In addition, when the shape of neck For cylinder or it is semicircular when, according to same reason, different projections can also be set between the wall of neck, from And form the passage of different in width.

In some preferable modes, tiny groove 312 is formed on the bottom (Fig. 4) of neck, the groove is through whole The bottom of neck (such as Fig. 3 is exactly the top of passage).So, when having the opening that liquid pours in passage, tiny is recessed Groove 312 can exclude gas in time, can faster allow the inside and outside balance for reaching air pressure in the passage of sealing, can To improve the time for reaching balance, allow liquid to reply tranquility faster, the influence of mighty torrent can also be reduced.

Further, with reference to rear Fig. 5, shown in 6, the length of whole neck or passage is 57.5 millimeters, detection reagent bar 60 millimeters of length, so allowing a part of reagent strip to be not in neck, but is exposed outside.Second width B of neck is 4.25 Millimeter.3rd width C is 3.25 millimeters, when with it is bell-mouthed when (it also cannot be with, such as shown in Figure 6), the One width A is 5.4 millimeters (Fig. 5), and the depth of neck all has different depth for identical depth or different parts.From figure 5, it is apparent that the width from the position of the 3rd width C to the first width A position be gradually it is increased, it is this gradually increase It can also be gentle increase or more violent increase to add, and this is included in the marrow of the present invention.In preferable side In formula, the width of opening is that gradually the height of increase or passage is also gradually increased, such as in Fig. 5, narrow ground Side 309 is sunken, and is then gradually increased width, to 308 opening position, the height of neck gradually increases also with the extension of wall Add, form a structure 311 for sticking up lip, so form relatively open area 310, right backward channel in the place of opening 305 It is interior, narrow region is formed, so may can more reduce mighty torrent phenomenon, reduces percussion of the localized liquid to reagent strip.

For example, in another way, such as (structure for not having mouth) shown in Fig. 6, the 3rd width C are 3,25 lis Rice is less than the second width B (4,25 centimetres), and it is 4.25 lis of the second width being consistently less than neck to have a segment structure in recess Rice, the structure of the second width is then progressivelyed reach, without bell-mouth structure of air (such as Fig. 5).But the width of reagent strip is 3.20 Or 3.1 mm in width.So, when reagent strip is arranged in neck, portion of reagent bar is at stenosis 309, Although reagent strip does not contact with the wall of neck, for passage, reagent strip is divided into two parts by stenosis, is leaned on The part and remote narrow part (such as at B) being closely open.When the passage containing reagent strip is (vertically, different in a variety of ways The inclination of angle) it is inserted into liquid sample or the detection means with passage (such as urine cup) receives liquid sample (urine), no matter urine is with which kind of angle, and speed and the how much of amount enter in urine cups, can significantly reduce mighty torrent phenomenon.

In some preferable modes, the structure of the flood control flow structure is in the lower section of reagent strip marked region, typically, There is waterline, expression reagent strip inserts to greatest extent either to be immersed or sample floods examination below the marked region of reagent strip The position line (such as the 509 of Fig. 1 E) of agent bar, then the structure of anti-mighty torrent is (such as at 331 and 330 in 309, Fig. 4 in Fig. 6 At 900 in structure, or Figure 1A) it is all located near waterline, such as maintain an equal level with waterline, or on waterline, But all in the place under marked region.

Embodiments of the invention is described in detail with reference to specific accompanying drawing.These specific examples of the implementation Only without prejudice to the limited list under spirit of the invention, it is not excluded that those of ordinary skill in the art prior art and The present invention with reference to and other caused specific embodiments.

Different detections is carried out in the present invention from a series of DOA products, mainly there is AMP (amphetamine), BAR (bar ratios It is appropriate), BZO (Benzodiazepine), COC (cocaine), MET (methamphetamine), MDMA (diformazan methylenedioxymethamphetamine aniline), MOP (morphine), OPI (morphine), OXY (hydroxyl dihydrocodeinone), MTD (methadone), PCP (Hog), PPX (propoxyhene), TCA (tricyclic antidepressant), THC (THC), COT (nicotine), BUP (buprenorphine), ACE (acetparaminosalol Phenol), KET (ketamine), MQL (methaqualone), EDDP (2- acetal -1,5 dimethyl -3,3- Diphenyl Pyrroles alkane).

The comparative experiments of mighty torrent phenomenon under 1 different notchs of embodiment

The first passage：The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection Reagent strip 500 forms.Passage is formed together with flat carrier including multiple notchs 30 on the carrier 100, neck 30 is length Square configuration, there is the setting of different in width, such as the schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C of neck on neck (3.25 millimeters) are less than the second width B (4.25 millimeters), but the second width B is less than the Breadth Maximum A (5.4 of the first width Millimeter), wherein the first width A is below the marked region of reagent strip and close to opening 305, wherein, the second width B of neck is big In the width of reagent strip, the first width A is suitable with the width of reagent strip or essence equal (width of reagent strip is 3.2 millis Rice), the other end of passage is sealing.Detection reagent bar 500 in the detection card, it is to be used for detecting in sample whether contain Poison Material, as hemp, cocaine etc. material, this when, the height or depth of neck are the same.

Second of passage (contrast, traditional notch)：Equally, compared with the first passage, difference is：We With the neck (the distance between corresponding wall in neck is all 4.25 mm wides) of same widths, the width of reagent strip is still For 3.20 millimeters, position of the reagent strip in neck is as in the first neck.

The third passage：Compared with the first passage, it is other all, unlike：Neck is rectangular shape, in card Setting with different in width on groove, such as the schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck are small In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., has on groove is examined recessed Fall into position (309), without bell-mouthed structure.Other positions are as the first neck, including reagent strip and the same Width.

Operating method：When the detection, it is detection 100 (the detection cards directly containing three of the above channel design) of card are straight Connect and be inserted into containing in a variety of small molecule drugs mixed solutions.Kept for 1 minute in liquid sample, it is determined that enough samples are used for Detection.Now, liquid sample can reach marked region 502, and mark along the sample application zone 503 of detection reagent bar 500 Mark substance on region is sufficiently mixed uniformly, detection zone 504 is then reached, in the test results zone of detection zone 504 Domain forms detection symbols, such as detection line, for judging whether contain analyte in liquid sample.Following table is mighty torrent number Statistical form, such as following table：

As can be seen from the above table, when the structure in neck or when passage occurs with flood control flow structure, In the neck of all experiments, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without Fluidic structures are limited in same neck or passage, the incidence of mighty torrent is 15%, and only 86.5% test-strips occur There is not control line, regardless of detection line, are all represented as invalid knot in control line, the test-strips for illustrating to also have 13.5% Fruit.Comparatively, the third passage occur mighty torrent probability it is much smaller, compared to the test in traditional existing channel design Bar.

Comparative experiments (in urine cup)-laboratory of mighty torrent phenomenon under 2 different notchs of embodiment

The first neck：The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection Reagent strip 500 forms.Passage is formed together with carrier including multiple notchs 30 on the carrier 100, neck is rectangle shape Shape, there is the setting of different in width, such as the schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 of neck on neck Millimeter) be less than the second width B (4.25 millimeters), but the second width B less than the first width A (5.4 millimeters) (bell-mouthed most Big width), wherein the 3rd width C is below the marked region 502 of reagent strip and close to opening 305, wherein, the second of neck is wide Spend B be more than reagent strip width, the 3rd width C it is suitable with the width of reagent strip or essence it is equal (width of reagent strip be 3.2 Millimeter), the other end of passage is sealing.Detection reagent bar 500 in the detection card, whether it is used to detect in sample containing poisonous Product material, such as hemp, cocaine etc. material.

Second of neck (contrast)：Equally, compared with the first neck, difference is：We use the card of same widths Groove (the distance between corresponding wall in neck is all 4.25 mm wides), the width of reagent strip is still 3.20 millimeters, examination Position of the agent bar in neck is as in the first neck.

The third neck：Compared with the first neck, it is other all, unlike：Neck is rectangular shape, in card Setting with different in width on groove, such as the schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 millimeters) of neck are small In the second width B (4.25 millimeters), but the second width B is but equal to the first width C (4.25 millimeters), i.e., has on groove is examined recessed Fall into position (309), it is other as the first neck without bell-mouthed structure, including reagent strip and the same width.

Operating method：When the detection, first the passages of three kinds of different structures (the first neck, second of neck, the Three kinds are examined groove) card bending (as shown in Figure 2), be inserted into the cup of urine cup, wherein, allow the opening of passage positioned at urine cup Bottom (Fig. 8), then quickly pouring into urine cup urine, (height of cup is 75 millimeters, and the length of whole passage is 57.5 Centimetre, the length of reagent strip is 60 millimeters), whole urine cup (is almost filled with liquid-like in 2 seconds by the volume of urine cup for 50 milliliters Product).Once being full of urine in urine cup, now, liquid sample can be entered in passage by the opening of passage, tried along detection The sample application zone 503 of agent bar 500, marked region 502 is reached, and mark substance on marked region is sufficiently mixed It is even, detection zone 504 is then reached, detection symbols is formed in the testing result region of detection zone 504, such as detection line, is used for Judge whether contain analyte in liquid sample.Following table be mighty torrent number statistical form, such as following table：

As can be seen from the above table, when the structure with flood control flow structure occurs in neck, in all examinations In the urine cup tested, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without limiting fluid Structure is in same neck or passage, and the incidence of mighty torrent is 17%, and control line, explanation occur in only 83% test-strips There is not control line in also 17% test-strips, regardless of detection line, are all represented as invalid result.Comparatively, The probability that mighty torrent occur in three kinds of passages is much smaller, compared to the test-strips in traditional existing channel design.

The comparative experiments (in urine cup) of mighty torrent phenomenon under 3 different notchs of embodiment-clinic

Operating method：Before detection is carried out, first the passages of three kinds of different structures (the first neck, second of neck, the Three kinds are examined groove) card bending (as shown in Figure 2), be inserted into the cup of urine cup (such as Fig. 8), wherein, allow the opening position of passage In the bottom of urine cup, then carrying out different people and carry out clinical trial, the people for exactly carrying out no professional operation is collected urine, Genitals are typically directly allowed to be urinated into urine cup into urine, so as to observe the probability of mighty torrent phenomenon generation, as a result such as following table：

As can be seen from the above table, when the structure with flood control flow structure occurs in neck, in all examinations In the urine cup tested, the first neck does not have the appearance of mighty torrent phenomenon, and control line occurrence rate is 100%, without limiting fluid Structure is in same neck or passage, and the incidence of mighty torrent is 20%, and control line, explanation occur in only 80% test-strips There is not control line in also 20% test-strips, regardless of detection line, are all represented as invalid result.Comparatively, The probability that mighty torrent occur in three kinds of passages is much smaller, compared to the test-strips in traditional existing channel design.

Examples of implementation 4：The experiment of mighty torrent phenomenon under extreme condition

The first neck：The present embodiment is related to a kind of detection card.Such as Fig. 2, the detection card 100 includes carrier 200 and detection Reagent strip 500 forms.Passage is formed together with carrier including multiple notchs 30 on the carrier 100, neck is rectangle shape Shape, there is the setting of different in width, such as the schematic diagram shown in Fig. 6 A and Fig. 5, the 3rd width C (3.25 of neck on neck Millimeter) be less than the second width B (4.25 millimeters), but the second width B less than the first width A (5.4 millimeters) (bell-mouthed most Big width), wherein the 3rd width C is below the marked region 502 of reagent strip and close to opening 305, wherein, the second of neck is wide Spend the width that B is more than reagent strip, the 3rd width C is suitable with the width of reagent strip or essence equal (width of reagent strip is 3.2 millimeters), the other end of passage is sealing.Detection reagent bar 500 in the detection card, it is to be used to detect in sample whether to contain Poison material, such as hemp, cocaine etc. material.

Certain urine is poured into cavity 90 in urine cup (Fig. 8), after formulation scale is circulated into, is then put into and shakes What progress was violent in bed rocks (frequency is that hz is per second), and the time rocked is 5 minutes or so, then removes, sees the result of experiment How, it is as a result as follows：

Under conditions of extremely acutely, the structure of the first neck or passage only has a generation mighty torrent phenomenon, still Control line still occurs, on the contrary, traditional channel design, because the structure for flow structure of not controlling flood, only 86.7% reagent There is control line in bar, and some does not occur, and illustrates to be used as invalid detection due to the influence of mighty torrent.Meanwhile the third It is more superior than traditional.

Claims

1. a kind of device for detecting analyte in fluid sample, the device include one end open, the passage that one end is closed, its In the passage be used to set detection reagent bar, wherein, passage includes flood control flow structure, and the structure allows the cross-sectional area of passage anti- Diminish at mighty torrent structure.

2. detection means according to claim 1, wherein, passage includes bottom plate and notch, its bottom plate and neck Structure formation one end open, the described passage of one end sealing, wherein, opening is attached positioned at the sample applying zone of detection reagent bar Closely.

3. detection means according to claim 1, wherein, the marked region on detection reagent bar is located at anti-mighty torrent in passage On structure.

4. detection means according to claim 1, wherein, bottom plate be it is transparent, plane, notch is nontransparent 's.

5. detection means according to claim 1, wherein, passage has been divided two different portions by the structure for limiting fluid Point, a part is first paragraph passage of the flood control flow structure to access portal, and another part is exactly to control flood flow structure to closed channel One end second segment passage, close to the place of opening, limit the structure of fluid and some position of passage become narrow, together When, the wall of passage stretches out along narrow place, and so extension is gradually to extend, so as to extension, the horizontal stroke of passage Section product namely gradually increase, so as to allow the cross-sectional area of one end passage in extension to be more than the cross section of second channel Product.

6. according to the detection means described in one of claim 1-5, wherein, described passage includes detection reagent bar, wherein The structure of limitation fluid is located under the marked region of reagent strip.

7. according to the detection means described in one of claim 1-6, wherein, described device also includes a receiving liquid sample Cavity, wherein, the passage is located in the cavity, wherein, described passage opening positioned at cavity bottom near.

8. detection means according to claim 2, wherein, described neck includes the neck of cuboid, and this is examined groove and divided For two parts, the Part I with neck opening and the Part II with blind end, wherein, Part I and second Divide the width of interface to diminish, form the first width.

9. detection means according to claim 8, wherein, opening forms the second width, and the 3rd is formed with blind end Width, wherein the first width is less than the 3rd width.

10. detection means according to claim 9, wherein, the 3rd section is less than the second width.