CN101929927B - 在组织处理器中自动处理组织样品的方法 - Google Patents
在组织处理器中自动处理组织样品的方法 Download PDFInfo
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- CN101929927B CN101929927B CN201010220984.6A CN201010220984A CN101929927B CN 101929927 B CN101929927 B CN 101929927B CN 201010220984 A CN201010220984 A CN 201010220984A CN 101929927 B CN101929927 B CN 101929927B
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- 238000000034 method Methods 0.000 title claims description 23
- 239000012876 carrier material Substances 0.000 claims abstract description 66
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 65
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 21
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 9
- 239000004215 Carbon black (E152) Substances 0.000 claims description 6
- 229930195733 hydrocarbon Natural products 0.000 claims description 6
- 150000002430 hydrocarbons Chemical class 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 4
- 230000008020 evaporation Effects 0.000 claims description 4
- 239000005662 Paraffin oil Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 150000003505 terpenes Chemical class 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 239000012188 paraffin wax Substances 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 240000004859 Gamochaeta purpurea Species 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002962 histologic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims (9)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102009025574A DE102009025574A1 (de) | 2009-06-19 | 2009-06-19 | Verfahren zum automatischen Bearbeiten von Gewebeproben in einem Gewebeprozessor |
DE102009025574.5 | 2009-06-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101929927A CN101929927A (zh) | 2010-12-29 |
CN101929927B true CN101929927B (zh) | 2015-11-25 |
Family
ID=42371081
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201010220984.6A Active CN101929927B (zh) | 2009-06-19 | 2010-06-21 | 在组织处理器中自动处理组织样品的方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20100323395A1 (zh) |
JP (1) | JP2011002453A (zh) |
CN (1) | CN101929927B (zh) |
DE (1) | DE102009025574A1 (zh) |
GB (1) | GB2471162B (zh) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102648404B (zh) | 2009-11-13 | 2015-07-22 | 徕卡仪器(新加坡)私人有限公司 | 微观样本处理装置系统状态的显示 |
JP6013376B2 (ja) * | 2011-02-28 | 2016-10-25 | ダコ・デンマーク・エー/エス | 包埋生体試料の前処置のための2相非混和系 |
WO2013192606A1 (en) | 2012-06-22 | 2013-12-27 | Leica Biosystems Nussloch Gmbh | Biopsy tissue sample transport device and method of using thereof |
US10092905B2 (en) | 2012-06-22 | 2018-10-09 | Leica Biosystems Nussloch Gmbh | Tissue sample container and methods |
GB2510466B (en) | 2012-12-28 | 2015-05-06 | Leica Biosystems Nussloch Gmbh | Processor for processing histological samples |
CA2845830C (en) | 2013-03-15 | 2020-10-27 | Leica Biosystems Nussloch Gmbh | Tissue cassette with retractable member |
US9389154B2 (en) | 2013-03-15 | 2016-07-12 | Leica Biosystems Nussloch Gmbh | Tissue cassette with biasing element |
US9052256B2 (en) | 2013-03-15 | 2015-06-09 | Leica Biosystems Nussloch Gmbh | Method for processing and embedding tissue |
AU2014363678B2 (en) | 2013-12-13 | 2016-12-08 | Ventana Medical Systems, Inc. | Staining reagents and other liquids for histological processing of biological specimens and associated technology |
CN104977194B (zh) * | 2014-04-10 | 2018-08-14 | 北京雷根生物技术有限公司 | 一种添加石墨烯加速样本处理的方法 |
US10591392B2 (en) | 2014-07-03 | 2020-03-17 | Applikate Technologies Llc | Simultaneous dehydration and staining of tissue for deep imaging |
US20230273100A1 (en) * | 2020-08-10 | 2023-08-31 | Leica Biosystems Nussloch Gmbh | Xylene-free tissue processor and method for processing tissue |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7070951B2 (en) * | 1994-03-11 | 2006-07-04 | Biogenex Laboratories | Dewaxing kit for immunostaining tissue specimens |
US7273720B1 (en) * | 2004-12-02 | 2007-09-25 | Collaborative Laboratory Services, Llc | Rapid tissue processing method and apparatus |
CN201063008Y (zh) * | 2007-08-02 | 2008-05-21 | 武汉钢铁(集团)公司 | 一种试样自动处理装置 |
CN101476999A (zh) * | 2009-01-23 | 2009-07-08 | 赵宝忠 | 一种甲醛固定液固定组织块的方法 |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2681298A (en) * | 1951-01-27 | 1954-06-15 | Technicon Chemical Company Inc | Preparation for and method of treating histological tissue |
US4911915A (en) * | 1987-10-13 | 1990-03-27 | Richard-Allan Medical Industries | Method of processing tissue specimens and dehydrant solvent for use therein |
DE4037518C2 (de) * | 1989-11-27 | 1998-12-17 | Toyo Boseki | Polymerteilchen, Verfahren zu deren Herstellung und Verwendung derselben |
US5344637A (en) * | 1993-06-01 | 1994-09-06 | Camiener Gerald W | Use of saturated, ring-containing compounds as clearing solvents in histologic procedures |
DK142193D0 (da) * | 1993-12-21 | 1993-12-21 | Esti Chem A S | Anvendelse af fedtsyreestere |
AU5382396A (en) * | 1995-03-31 | 1996-10-16 | Life Resuscitation Technologies, Inc. | Histological processing device and method |
EP0822403B1 (en) * | 1996-08-02 | 1998-07-29 | Milestone S.r.l. | Process for processing organic specimens |
CA2301924C (en) * | 1997-08-20 | 2008-12-09 | The University Of Miami | A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method |
JP2000258318A (ja) * | 1999-03-09 | 2000-09-22 | Muto Kagaku Kk | 溶媒置換用組成物及びこれを用いた標本作成方法 |
ATE551606T1 (de) * | 2001-10-01 | 2012-04-15 | Leica Biosystems Melbourne Pty | Gewebeprozessor zur behandlung histologischer gewebepräparate |
JP4871722B2 (ja) * | 2003-07-25 | 2012-02-08 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | 固定試料からrnaを調製するための方法および組成物 |
EP1673608A4 (en) * | 2003-09-29 | 2008-05-07 | Vision Biosystems Ltd | SYSTEM AND METHOD FOR PROCESSING HISTOLOGICAL TISSUE SPECIMENS |
KR20060115366A (ko) * | 2003-10-24 | 2006-11-08 | 더 유니버시티 오브 마이애미 | 간소화시킨 조직 가공 |
US7273587B1 (en) * | 2004-12-02 | 2007-09-25 | Collaborative Laboratory Services, Llc | Rapid tissue processing method and apparatus |
EP1851525B1 (en) | 2005-02-25 | 2014-11-26 | Leica Biosystems Melbourne Pty Ltd | Method and apparatus for tissue processing |
DE102005057191B4 (de) | 2005-11-29 | 2011-12-08 | Leica Biosystems Nussloch Gmbh | Gewebeprozessor |
US7666620B2 (en) * | 2006-11-08 | 2010-02-23 | Richard-Allan Scientific Company | Rapid tissue processing system |
JP4059520B1 (ja) * | 2007-07-25 | 2008-03-12 | 株式会社常光 | 組織片処理装置 |
DE102008039861A1 (de) * | 2008-08-27 | 2010-03-04 | Leica Biosystems Nussloch Gmbh | Gewebeprozessor |
DE102008039875B4 (de) * | 2008-08-27 | 2014-08-14 | Leica Instruments (Singapore) Pte. Ltd. | Verfahren und Vorrichtung zum Infiltrieren von Gewebeproben mit Paraffin |
DE102008054066B4 (de) * | 2008-10-31 | 2015-03-19 | Leica Biosystems Nussloch Gmbh | Verfahren zum Bearbeiten von Gewebeproben unter Verwendung eines Sensors |
-
2009
- 2009-06-19 DE DE102009025574A patent/DE102009025574A1/de active Pending
-
2010
- 2010-05-26 GB GB1008869A patent/GB2471162B/en active Active
- 2010-05-27 US US12/788,604 patent/US20100323395A1/en not_active Abandoned
- 2010-06-14 JP JP2010134824A patent/JP2011002453A/ja active Pending
- 2010-06-21 CN CN201010220984.6A patent/CN101929927B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7070951B2 (en) * | 1994-03-11 | 2006-07-04 | Biogenex Laboratories | Dewaxing kit for immunostaining tissue specimens |
US7273720B1 (en) * | 2004-12-02 | 2007-09-25 | Collaborative Laboratory Services, Llc | Rapid tissue processing method and apparatus |
CN201063008Y (zh) * | 2007-08-02 | 2008-05-21 | 武汉钢铁(集团)公司 | 一种试样自动处理装置 |
CN101476999A (zh) * | 2009-01-23 | 2009-07-08 | 赵宝忠 | 一种甲醛固定液固定组织块的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN101929927A (zh) | 2010-12-29 |
DE102009025574A1 (de) | 2010-12-23 |
GB2471162A (en) | 2010-12-22 |
GB201008869D0 (en) | 2010-07-14 |
JP2011002453A (ja) | 2011-01-06 |
US20100323395A1 (en) | 2010-12-23 |
GB2471162B (en) | 2011-11-16 |
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Legal Events
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ASS | Succession or assignment of patent right |
Owner name: LEICA INSTRUMENTS (SINGAPORE) PTE. LTD. Free format text: FORMER OWNER: LEICA BIOSYSTEMS NUSSLOCH GMBH Effective date: 20121023 |
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C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20121023 Address after: Singapore Singapore Applicant after: Leica Biosystems Nussloch GmbH Address before: German Noons Lo Beh Applicant before: Leica Biosystems Nussloch GmbH |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20180704 Address after: German Noons Lo Beh Patentee after: Leica Biosystems Nussloch GmbH Address before: Singapore Singapore Patentee before: Leica Biosystems Nussloch GmbH |