CN101928317A - Extraction of active ingredients of cordyceps sinensis and application of active ingredients to cigarettes - Google Patents
Extraction of active ingredients of cordyceps sinensis and application of active ingredients to cigarettes Download PDFInfo
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- CN101928317A CN101928317A CN 201010258714 CN201010258714A CN101928317A CN 101928317 A CN101928317 A CN 101928317A CN 201010258714 CN201010258714 CN 201010258714 CN 201010258714 A CN201010258714 A CN 201010258714A CN 101928317 A CN101928317 A CN 101928317A
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Abstract
The invention discloses extraction of active ingredients of cordyceps sinensis and application active ingredients to cigarettes thereof, and relates to extraction of active ingredients from the cordyceps sinensis and an application method of the active ingredients to cigarettes. The active ingredients of the cordyceps sinensis are prepared by the following steps of: pretreating the cordyceps sinensis, performing water extraction or alcohol extraction, filtering and concentrating, and performing membrane separation and column chromatography, wherein the obtained substances are cordycepic acid, cordycepin and adenosines; and one or more of the active ingredients of the cordyceps sinensis are dissolved and added into cut tobacco. Tests prove that: compared with contrast cigarettes, the cigarettes in the invention have the transfer rate of the active ingredients of the cordyceps sinensis of 5 to 10 percent in mainstream cigarette smoke, reduce the content of some harmful components in the mainstream cigarette smoke by 20 to 45 percent, such as smoke benzpyrene, nitrosamine specific to tobacoo, smoke gas phase free radicals, and the like, particularly have better effect of reducing the smoke gas phase free radicals for the cigarettes, and have the advantages of no peculiar smell during smoking and functions of promoting the production of body fluid, soothing the throat, eliminating phlegm and the like.
Description
Technical field
A kind of extraction of active ingredients of cordyceps sinensis and the application in cigarette thereof relate to and extract active ingredients of cordyceps sinensis and the application method in cigarette thereof from Cordyceps sinensis.
Background technology
Along with the problem of smoking with health more and more receives publicity, the objectionable constituent of sucking security, increase nourishing function, reducing in the cigarette smoke that improve cigarette are the inevitable development trend of cigarette.
China is the native place that utilizes herbal medicine, is to find and utilize herbal medicine country the earliest, and herbal medicine is the rarity of the Chinese nation, and the long history in thousands of years is arranged.With the medicine gasification, hear method treatment illness, the especially bronchitis and the upper respiratory tract infection of inhaling with burning, at the existing nearly 3,000 years literature record of China.Inspired by " sootiness therapy " scheduling theory in motherland's medicine and pharmacology, we can seek Chinese herbal medicine resource, the burst size of selectivity reducing cigarette fume objectionable constituent in the hope of not influencing the cigarette organoleptic quality from intrinsic advantage.Therefore adding herbal medicine falls the developing direction that harmful composition has become the Chinese style cigarette, utilizes herbal medicine to carry out lowering harm and decreasing coking at China's tobacco industry in recent years and has obtained bigger progress.
Cordyceps sinensis is the crucial medicinal fungi of a class, be to parasitize the entomogenous fungi complex body that fungi on the insect body and its host insect form, be under the jurisdiction of Ascomycetes (Ascomycetes), Clavicipitaceae (Claricipitaceae), Cordyceps (Cordyceps).Studies show that in recent years: Cordyceps sinensis have calmness, antibacterial, suppress many-sided pharmacological actions such as mouse ascites cancer, enhancing body immunologic function.Cordyceps sinensis has various active composition (for example cordycepic acid, cordycepin and adenosine etc.), and these activeconstituentss have pharmacological action widely.
The Cordyceps sinensis cordycepin: have antibiotic, anti-inflammatory, anti-oxidant, antitumor, regulate effect such as human endocrine.
Cordyceps sinensis adenosine: have the function that participates in body metabolism and physiological regulation.
Cordyceps sinensis cordycepic acid: recent clinical, pharmacological research report, cordycepic acid can suppress the growth of various germs, can prevent and treat cerebral thrombosis, hematencephalon, myocardial infarction, long-term depleted, cordycepic acid can be protected lung kidney-nourishing, hemostasis and phlegm, obviously strengthen segmental bronchus cilia activity ability, regulate bronchial smooth muscle, and can remove the strongest hydroxy radical qiao of toxicity in the human body, and numerous diseases are had obvious curative effects.This have with Cordyceps sinensis invigorate the lung and the kidney, hemostasis and phlegm, the effect that improves the health be consistent.
Only be to study and use at the chemical constituents determination of the Cordyceps sinensis worm being removed from office acid, cordycepin and adenosine, medical etc. about research both at home and abroad at present, not finding should have precedent in tobacco.
Summary of the invention
The objective of the invention is to join in the cigarette shreds, to reduce the harm of cigarette to human body with behind the active ingredients of cordyceps sinensis extraction separation.
The present invention seeks to realize that by following scheme active ingredients of cordyceps sinensis of the present invention is to be made by following method:
(1) Cordyceps sinensis is crushed to the 10-100 order after the oven dry down at 30-50 ℃ after washing, picking out the pre-treatment of impurity, airing;
(2) under 80-100 ℃ or ultrasonic wave or microwave condition, water or concentration are that 20-80% ethanol extracts 1-10h to the Cordyceps sinensis raw material;
(3) behind the filtration under diminished pressure, be 80% with obtaining the content that filtrate decompression is concentrated to dry-matter;
(4) will concentrate feed liquid at first behind membrane sepn, be moving phase with 20-40% ethanol again, separate, flow out cordycepic acid, cordycepin, adenosine successively through column chromatography (silicagel column, alumina column or macroporous resin etc. are filler), concentrate respectively then, can obtain the single active ingredient of Cordyceps sinensis worm;
To obtain the single active ingredient of Cordyceps sinensis or by cordycepic acid: cordycepin: adenosine=4～1: composite effective constituent was directly added in the pipe tobacco in 1: 0.2, in every cigarette shreds the total amount of active ingredients of cordyceps sinensis by 0.4 μ g/ prop up～40 μ g/ prop up and add cigarette shreds to;
The effective constituent of Cordyceps sinensis of the present invention is added on the cigarette shreds with feed liquid as cigarette behind water or 10-90% dissolve with ethanol; Concrete usage ratio is: the effective constituent of Cordyceps sinensis content in pipe tobacco be 0.4 μ g/ prop up～40 μ g/ prop up.The effective constituent water soluble of Cordyceps sinensis and 10-90% ethanol, strength of solution is 1-10% when using, by the spray add mode the single active ingredient of Cordyceps sinensis or cordycepic acid: cordycepin: adenosine=4～1: composite effective constituent was evenly added on the pipe tobacco in 1: 0.2.
Substantive features of the present invention are to utilize the effective constituent of Cordyceps sinensis to burn and suck by cigarette to enter main flume, the peculiar chemical ingredients that cracking simultaneously produces, obviously improve or improve the quality quality of cigarette, have the effect of promoting the production of body fluid significantly and certain relieving cough function, can reduce the content of some objectionable constituent in the cigarette mainstream flue gas, and do not change original performance of cigarette, do not change or do not influence the sucking quality of cigarette, therefore have the meaning and the using value of important actual production.
The present invention has the following advantages compared to existing technology:
1. can improve the sucking quality of cigarette;
2. can reduce some objectionable constituent in the cigarette mainstream flue gas;
3. can make effective constituent be transferred to main flume;
4. has effects such as promoting the production of body fluid, wet one's whistle, eliminate the phlegm, reduce stimulation;
5. without any side effects.
After tested: compared with the contrast cigarette by the cigarette that the present invention rolled, active ingredients of cordyceps sinensis is 5-10% in the rate of transform of main flow cigarette smoke; The content of some objectionable constituent in the main flume, as flue gas benzopyrene, tobacco-specific nitrosamine, smoke gaseous phase free radical etc., the reduction amplitude is between 20-45%, and especially effect is better aspect reduction cigarette smoke free radical; When cigarette burns and sucks, the flue gas free from extraneous odour, have promote the production of body fluid, wet one's whistle, effect such as sharp phlegm.
Embodiment
Embodiment 1:
Get the Cordyceps sinensis dry product, wash with water clean, pick out impurity after, dry or dry; After oven dry under 40 ℃, be crushed to the 10-100 order; Under 90 ℃ of conditions, with water extraction Cordyceps sinensis 6h, after the filtration, remove impurity, with after obtaining content that filtrate is concentrated to dry-matter and being 80% through membrane sepn, concentrated solution is placed the chromatography column upper end, with 90% ethanol is moving phase, and effective constituents such as cordycepic acid, adenosine, cordycepin are isolated in the thin-layer chromatography monitoring successively, after concentrating respectively, can obtain single active ingredient of the present invention.
Cordyceps sinensis cordycepic acid, adenosine, cordycepin join on the cigarette shreds with feed liquid through making cigarette after the water dissolution, the ratio that specifically applies is: the consumption of Cordyceps sinensis cordycepic acid be 16 μ g/ prop up～40 μ g/ prop up, the consumption of Cordyceps sinensis adenosine be 0.4 μ g/ prop up～16 μ g/ prop up, the consumption of Cordyceps sinensis cordycepin be 0.4 μ g/ prop up～12 μ g/ prop up, be added on the pipe tobacco by the spray add mode behind the dilute with water.
Embodiment 2:
Get the Cordyceps sinensis dry product, wash with water clean, pick out impurity after, dry or dry.After oven dry under 40 ℃, be crushed to the 10-100 order.Under the ultrasonic wave condition, with 20-80% extraction using alcohol Cordyceps sinensis 2h, remove impurity through membrane sepn, the content that filtrate is concentrated to dry-matter is that 80% back is a moving phase with 90% ethanol, the thin-layer chromatography monitoring, isolate effective constituents such as the acid of worm leather, adenosine, cordycepin successively, after concentrating respectively, can obtain single active ingredient of the present invention.
Cordyceps sinensis cordycepic acid, adenosine, cordycepin join on the cigarette shreds with feed liquid through making cigarette after the water dissolution, the ratio that specifically applies is: the consumption of Cordyceps sinensis cordycepic acid is that 16～40 μ g/ of pipe tobacco weight prop up, the consumption of Cordyceps sinensis adenosine is that 0.4～16 μ g/ of pipe tobacco weight props up, the consumption of Cordyceps sinensis cordycepin is that 0.4～12 μ g/ of pipe tobacco weight props up, and is added on the pipe tobacco by the spray add mode behind the dilute with water.
Claims (5)
1. the extraction of an active ingredients of cordyceps sinensis and the application in cigarette thereof is characterized in that active ingredients of cordyceps sinensis is to be made by following method:
(1) Cordyceps sinensis is crushed to the 10-100 order after the oven dry down at 30-50 ℃ after washing, picking out the pre-treatment of impurity, airing;
(2) under 80-100 ℃ or ultrasonic wave or microwave condition, water or concentration are that 20-80% ethanol extracts 1-10h to the Cordyceps sinensis raw material;
(3) behind the filtration under diminished pressure, be 80% with obtaining the content that filtrate decompression is concentrated to dry-matter;
(4) will concentrate feed liquid at first behind membrane sepn, again through column chromatography for separation, concentrate after, i.e. Cordyceps sinensis cordycepic acid, cordycepin, adenosine.
2. the extraction of active ingredients of cordyceps sinensis according to claim 1 and the application in cigarette thereof, the cordycepic acid that it is characterized in that obtaining Cordyceps sinensis props up-40 μ g/ by 0.4 μ g/ and props up and add cigarette shreds to.
3. the extraction of active ingredients of cordyceps sinensis according to claim 1 and the application in cigarette thereof, the adenosine that it is characterized in that obtaining Cordyceps sinensis props up-16 μ g/ by 0.4 μ g/ and props up and add cigarette shreds to.
4. the extraction of active ingredients of cordyceps sinensis according to claim 1 and the application in cigarette thereof, the cordycepin that it is characterized in that obtaining Cordyceps sinensis props up-12 μ g/ by 0.4 μ g/ and props up and add cigarette shreds to.
5. the extraction of active ingredients of cordyceps sinensis according to claim 1 and the application in cigarette thereof, it is characterized in that cordycepic acid: cordycepin: adenosine=4～1: 1: 0.2 composite, obtains composite product and prop up-40 μ g/ by 0.4 μ g/ and prop up and add cigarette shreds to.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010102587144A CN101928317B (en) | 2010-08-13 | 2010-08-13 | Extraction of active ingredients of cordyceps sinensis and application of active ingredients to cigarettes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102587144A CN101928317B (en) | 2010-08-13 | 2010-08-13 | Extraction of active ingredients of cordyceps sinensis and application of active ingredients to cigarettes |
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| CN2012102809295A Division CN102835728A (en) | 2010-08-13 | 2010-08-13 | Applications of active ingredients of cordyceps sinensis in cigarettes |
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| CN101928317A true CN101928317A (en) | 2010-12-29 |
| CN101928317B CN101928317B (en) | 2012-11-21 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054156A (en) * | 2012-12-18 | 2013-04-24 | 内蒙古昆明卷烟有限责任公司 | Application of cordyceps sinensis extraction residues to reconstituted tobacco |
| CN105725271A (en) * | 2016-04-14 | 2016-07-06 | 红云红河烟草(集团)有限责任公司 | Filter stick containing filter screen sheet loaded with cordyceps sinensis extract |
| WO2016107776A1 (en) * | 2014-12-31 | 2016-07-07 | Nestec S.A. | A process for preparing cordyceps extract |
| CN113308384A (en) * | 2021-07-15 | 2021-08-27 | 四川中烟工业有限责任公司 | Cordyceps sinensis strain and application thereof |
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2010
- 2010-08-13 CN CN2010102587144A patent/CN101928317B/en active Active
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| CN1297774A (en) * | 1999-12-01 | 2001-06-06 | 上海国宝企业发展中心 | Extraction of effective component in Northern cordyceps producing matrix |
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| CN101015571A (en) * | 2007-03-13 | 2007-08-15 | 陈林生 | Chinese caterpillar fungus polysaccharide buccal tablets and its preparing process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054156A (en) * | 2012-12-18 | 2013-04-24 | 内蒙古昆明卷烟有限责任公司 | Application of cordyceps sinensis extraction residues to reconstituted tobacco |
| WO2016107776A1 (en) * | 2014-12-31 | 2016-07-07 | Nestec S.A. | A process for preparing cordyceps extract |
| CN107105736A (en) * | 2014-12-31 | 2017-08-29 | 雀巢产品技术援助有限公司 | The method for preparing Cordyceps |
| CN105725271A (en) * | 2016-04-14 | 2016-07-06 | 红云红河烟草(集团)有限责任公司 | Filter stick containing filter screen sheet loaded with cordyceps sinensis extract |
| CN113308384A (en) * | 2021-07-15 | 2021-08-27 | 四川中烟工业有限责任公司 | Cordyceps sinensis strain and application thereof |
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| Publication number | Publication date |
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| CN101928317B (en) | 2012-11-21 |
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