CN107105736A - The method for preparing Cordyceps - Google Patents
The method for preparing Cordyceps Download PDFInfo
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- CN107105736A CN107105736A CN201580071335.5A CN201580071335A CN107105736A CN 107105736 A CN107105736 A CN 107105736A CN 201580071335 A CN201580071335 A CN 201580071335A CN 107105736 A CN107105736 A CN 107105736A
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- cordyceps
- minutes
- cordyceps sinensis
- temperature
- bleaching
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- 241000190633 Cordyceps Species 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000000203 mixture Substances 0.000 claims abstract description 22
- 239000000284 extract Substances 0.000 claims abstract description 11
- 150000003838 adenosines Chemical class 0.000 claims abstract description 9
- 206010061218 Inflammation Diseases 0.000 claims abstract description 4
- 230000004054 inflammatory process Effects 0.000 claims abstract description 4
- 230000036039 immunity Effects 0.000 claims abstract description 3
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 102
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 238000004061 bleaching Methods 0.000 claims description 28
- 241001264174 Cordyceps militaris Species 0.000 claims description 21
- 235000013305 food Nutrition 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 238000000227 grinding Methods 0.000 claims description 9
- 235000013361 beverage Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000002778 food additive Substances 0.000 claims description 4
- 235000013373 food additive Nutrition 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 4
- 206010033546 Pallor Diseases 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 235000013402 health food Nutrition 0.000 claims 1
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 abstract description 72
- 239000002126 C01EB10 - Adenosine Substances 0.000 abstract description 36
- 229960005305 adenosine Drugs 0.000 abstract description 36
- 239000002002 slurry Substances 0.000 description 34
- 239000000843 powder Substances 0.000 description 18
- 230000035764 nutrition Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000003389 potentiating effect Effects 0.000 description 6
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 6
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 5
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 5
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000919 ceramic Substances 0.000 description 4
- 238000003801 milling Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 241000625793 Cordyceps pruinosa Species 0.000 description 3
- 241000099774 Cuscuta salina Species 0.000 description 3
- 240000001307 Myosotis scorpioides Species 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000012925 reference material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 2
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000009928 pasteurization Methods 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 244000283482 Alloteropsis cimicina Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000006698 Spigelia anthelmia Species 0.000 description 1
- 241001236266 Tolypocladium ophioglossoides Species 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical class C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000003293 cardioprotective effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- -1 gglomerate Species 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002212 purine nucleoside Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Nutrition Science (AREA)
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- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
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Abstract
The invention discloses the method for preparing the Cordyceps comprising high concentration adenosine.The extract includes 100mg 220mg adenosines/100g.The extract can be used for preparing the alimentation composition for treating or preventing fatigue, angiocardiopathy or inflammation or improving immunity.
Description
Technical field
The present invention relates to the method for preparing Cordyceps.More particularly, it relates to be included for preparation
The method of the Cordyceps of high concentration adenosine.
Background technology
Any discussion of prior art is not construed as recognizing that this prior art is widely known absolutely in this specification
Or constitute a part for general knowledge known in this field.
Cordyceps sinensis (Cordyceps) is the main sac fungus fungi grown with insect and other arthropods.Cordyceps sinensis
It is well-known traditional Chinese medicine composition.Cordyceps sinensis is also referred to as cordyceps sinensis, English name for " caterpillar fungus " or
“winter worm summer grass”.When in traditional Chinese medicine in use, this composition actually by fungi together with thereon
Parasitic moth larvae composition.In the remainder of this specification, term " cordyceps sinensis " refers to traditional medicine composition, such as in tradition
It is used in medicine.When referring to fungi strain, the title initial of strain or category should be capitalized and be represented with italic, such as Cordyceps Militaris
(Cordyceps sinensis)。
According to traditional Chinese medicine, cordyceps sinensis is commonly used for tonic, and cures purpose and for disappearing for kidney tonifying, beneficial lung etc.
Except fatigue.Because Cordyceps Militaris is rare and curative effect outstanding, some bacterial strains are isolated from wild type Chinese caterpillar fungus hypha.These separation
Bacterial strain be used for zymotechnique, then usually as functional food products sell.The quality control pair of Cordyceps Militaris and products thereof
In ensuring that its security and effect are most important.In addition, also using some substitutes such as Cordyceps militaris (C.militaris) and
Doping cordyceps sinensis (C.adulterants).
The cordyceps sinensis seemingly sense such as cordycepin (cordycepin), adenosine and its derivative, ergosterol or polysaccharide is emerging
The source of the chemical constituent of interest.
Adenosine is a kind of purine nucleosides, in Biochemical processes such as energy transmission (as adenosine triphosphate (ATP) and
Adenosine diphosphate (ADP)) and play a significant role in signal transduction (being used as 3'5'-AMP salt (cAMP)).It is also
A kind of neuromodulator, for promoting sleep and suppressing excited.Adenosine is also flowing to the blood of each organ by vasodilation regulation
Played a significant role during stream.Adenosine passes through the several types in for example immune, nerve, circulation, breathing or urinary system
Acceptor works.Research points out, adenosine and cordycepin be some bioactive compounds there is provided the anti-inflammatory of cordyceps sinensis, it is anti-oxidant and
Cardioprotective curative effect.
It has been generally acknowledged that cordycepin (cordycepin) and adenosine are the quality symbol things of cordyceps sinensis.Fresh cordyceps sinensis is also comprising big
Measure water.Therefore, for business and for a long time, cordyceps sinensis must be dried by leaving with purpose.In addition, having to comply with method for edible cordyceps sinensis
Rule require.For example, in China, worm grass product must include at least 50mg/100g adenosine and have less than 10000
Total plate count (TPC).
Research shows for many years, the characteristic and nutrition composition of the Cordyceps Militaris manually cultivated and the characteristic of wild Chinese caterpillar fungus bacterium and nutrition
Component is identical, or stronger.It is another to produce spore by artificial culture there are some researches show Cordyceps Militaris, and spore is considered as Cordyceps Militaris
The cream of the crop.
Preparing the conventional method of cordyceps sinensis includes drying, grind, sterilize and packing.However, the cordyceps sinensis traditionally prepared
Extract is without required adenosine content and is unsatisfactory for microbial safety standard.In order that finished product has high adenosine concentration,
Supplier must be very careful when selecting fresh cordyceps sinensis and technological parameter.However, these operations are there is no guarantee that product
Uniformity, especially reproducible batch quality.
In addition, in order to eliminate microbiological hazards, the commonly used sterilizing of supplier or radiation.However, these operations may draw
Adenosine loss or other active component performance degradations are played, may then cause compliance problem.
Wish to overcome or improve at least one shortcoming of prior art, or useful alternative solution is provided.Specifically, wish
Hope to provide and prepare the method with consistent batch quality, enough adenosine contents and the Cordyceps of humble biological pollution.
Food product or beverage, such as functional food products are can be readily made it is desirable also to Cordyceps.
The content of the invention
The purpose of the present invention is realized by the theme of independent claims.Dependent claims further expand the present invention's
Conception.
Therefore, in a first aspect, the present invention is provided to prepare the method for Cordyceps, this method includes following step
Suddenly：A) cordyceps sinensis is ground, the cordyceps sinensis b) is bleached, the cordyceps sinensis of the bleaching c) is dried, wherein bleaching is included in 27 DEG C to 90 DEG C
At a temperature of the cordyceps sinensis is handled in water 20 to 240 minutes.
In second aspect, the present invention provides the Cordyceps prepared using method according to a first aspect of the present invention, its
Described in Cordyceps include 100mg-220mg adenosines/100g extracts.
In the third aspect, the present invention is provided to be extracted comprising the cordyceps sinensis that can be obtained by method according to a first aspect of the present invention
The alimentation composition of thing or Cordyceps according to a second aspect of the present invention.The alimentation composition can for food product, beverage,
Functional health care food, nutritional agents, food additives, nutrient formulation and pet food.
In fourth aspect, the present invention provides Cordyceps according to a second aspect of the present invention or by according to the present invention the
Cordyceps prepared by the method for one side, which is used to prepare to treat or prevent fatigue, angiocardiopathy or inflammation or improve, exempts from
The purposes of the alimentation composition of epidemic disease power.
Those skilled in the art is after the detailed description to embodiment of the present invention is read in conjunction with the figure, will become more apparent that ground
Understand these and other aspects, features and advantages of the present invention.
Brief description of the drawings
Fig. 1 is the flow chart for showing cordyceps sinensis traditional preparation methods, as described in example 1 above (prior art).
Fig. 2 to Fig. 5 is the flow chart for showing the embodiment for preparing Cordyceps method according to the present invention, is such as implemented
Described in example 2 to 5.
Fig. 6 is the chromatogram that UPLC-PDA analyzes gained Cordyceps, as described in test example 6.
Embodiment
Through this specification, word " comprising ", "comprising" etc. all should be interpreted that opposite with exclusiveness or exhaustive implication
Inclusive implication, that is to say, that be the meaning of " including but is not limited to ".As used in this specification, singulative " one/mono-
Kind ", " should/described " include plural thing, unless linguistic context clearly refers else.
Unless otherwise stated, all percentages in this specification all refer to percentage by weight.
Unless otherwise defined, otherwise all scientific and technical terminologies all have and should be endowed with it is of the art general
The implication identical implication that logical technical staff is generally understood that.
Cordyceps sinensis used herein refers to all cordyceps species, including Cordyceps Militaris, Cordyceps militaris, head cordyceps sinensis (C.capita),
Cordyceps pruinosa (C.pruinosa) and Goldenthread Cordyceps (C.ophioglossoides).In one embodiment, cordyceps species
Selected from Cordyceps Militaris, Cordyceps militaris, head cordyceps sinensis, Cordyceps pruinosa and Goldenthread Cordyceps, Cordyceps Militaris, Cordyceps militaris and agglomerate are preferably chosen from
Cysticercus grass.
When preparing the Cordyceps according to the present invention, the mixing of different cordyceps species can be used, such as including cordyceps sinensis
The mixing cordyceps sinensis of both bacterium and Goldenthread Cordyceps.Preferably, cordyceps sinensis is fresh.So, the quality of Cordyceps is compared
Cordyceps sinensis is dried to make moderate progress.However, equally Cordyceps can be prepared based on cordyceps sinensis is dried.
The example of method for preparing Cordyceps is shown in Fig. 2 into Fig. 5.In Fig. 2 into Fig. 5, optional step with
Dotted line frame is represented.Grey box corresponds to blanching step, as explained further on.
For all embodiments of Cordyceps preparation method, identical is a little that cordyceps sinensis is pulverized
End.It can be ground in water, to directly obtain cordyceps sinensis slurries.Grinding increases the surface/volume of cordyceps sinensis particle, so as to carry
High extraction efficiency.Grinding can by chop, cut or other methods realize.Preferably, cordyceps sinensis is ground to form to 0.5mm to 5mm grain
Footpath, more preferably 1mm to 3mm particle diameter.Preferably, using fresh cordyceps sinensis, but dry cordyceps sinensis can also be used.
As shown in Fig. 2 to Fig. 5 flow chart, water is added in cordyceps sinensis after grinding, to obtain cordyceps sinensis slurries.As before
It is literary described, it can also add water to before the grinding in cordyceps sinensis.The weight ratio of cordyceps sinensis and water is not fixed.Usual cordyceps sinensis and the weight of water
Than in the range of 0.5: 1 to 2: 1, and preferably in the range of 1: 1 to 1.5: 1.If using less water, extracting effect
Rate may be relatively low.If using too many water relative to cordyceps sinensis, excessive water must be evaporated, and this needs to consume more multipotency
Amount.Water can be distilled water or running water.Preferably, the risk of microorganism pollution is reduced using distilled water, especially not right
When Cordyceps sterilizes.
Then cordyceps sinensis slurries are bleached in water.Bleaching handles the cordyceps sinensis at a temperature of being included in 27 DEG C to 90 DEG C in water
20 to 240 minutes.It has been found by the present inventors that bleaching cordyceps sinensis slurries improve the adenosine content of Cordyceps.Blanching step
Duration depends on bleaching temperature.
In some embodiments, bleaching is completed in one step.For example, bleaching worm at a temperature of 30 DEG C to 85 DEG C
Straw pulp liquid 30 minutes to 200 minutes, bleaches cordyceps sinensis slurries 120 minutes to 200 minutes preferably at a temperature of 30 DEG C to 45 DEG C.
In one embodiment, cordyceps sinensis slurries are bleached by soaking 150 minutes at a temperature of 30 DEG C to 40 DEG C to 200 minutes.
In another embodiment, cordyceps sinensis slurries are bleached at a temperature of 70 DEG C to 90 DEG C 30 minutes to 60 minutes.
In a further preferred embodiment, bleaching is divided into two steps：The first step is gently is heat-treated in water, then
It is potent heat treatment in water to carry out second step.For example, cordyceps sinensis slurries 120 are soaked in water first at a temperature of 30 DEG C to 45 DEG C
Slurries, to 200 minutes, are then heated to 70 DEG C to 90 DEG C of temperature and kept again in water 30 minutes to 60 minutes by minute.
In another preferred embodiment, the bleaching of cordyceps sinensis slurries is accomplished by the following way：30 DEG C to 45 DEG C first
At a temperature of cordyceps sinensis slurries are soaked in water 120 minutes to 200 minutes, then elevated the temperature in 5 to 15 minutes to 70 DEG C extremely
90 DEG C of second temperature, and keep second temperature 30 to 45 minutes.
It will such as be shown in embodiment, it has been found by the present inventors that it is (gentle hot cordyceps sinensis slurries to be soaked at 30 DEG C 180 minutes
Processing), cordyceps sinensis slurries are then heated to 85 DEG C and 30 minutes (potent heat treatment) is kept, can prepare and contain with highest adenosine
The extract of amount.
It is preferred that being sterilized before the drying to bleached cordyceps sinensis slurries.Sterilize for reducing potential pollution and making the guarantor of product
The matter phase is longer.Bleached cordyceps sinensis slurries are sterilized 10 to 15 minutes, more preferably from about 15 points at a temperature of 98 DEG C to 101 DEG C
Clock.
After sterilization, cordyceps sinensis slurries are for example dried by freeze-drying, oven method or boulton process.Can
Dried cordyceps sinensis is ground to form into 30 to 200 mesh sizes, and the more preferably powder of 50 to 100 mesh sizes.
When preparing Cordyceps by the above method, it can obtain including the Cordyceps of high concentration adenosine, for example often
In 100g Cordyceps 100mg to 220mg adenosines concentration.This is far above the 25mg/ according to obtained by previous preparation method
100g concentration (Fig. 1 and Examples below 1).In some embodiments, it can reach higher than 110mg/100g, and even above
150mg/100g adenosine concentration.The ultimate density of adenosine depends on conditions of bleaching, including bleaching time and temperature and bleaching
Step.
An aspect of of the present present invention is related to the alimentation composition for including the Cordyceps obtained using the above method.The battalion
Foster composition can be food product, beverage, functional health care food, nutritional agents, food additives, nutrient formulation and pet food.
The alimentation composition can be prepared by the way that Cordyceps is mixed into Nutrient product.When needing dry type nutrition product
When, it can will dry Cordyceps dry-mixed into dry nutrition product.For example, can will dry Cordyceps is mixed into milk powder
In.When needing wet type nutrition product, it may be desirable to before wet type nutrition product is sterilized or is heat-treated, after bleaching
Cordyceps sinensis slurries (may by concentration or sterilize) be mixed into wet type nutrition product.
In fourth aspect, the present invention provides contained adenosine amount scope in 100mg/100g to the cordyceps sinensis between 220mg/100g
Extract is used for the use for preparing the alimentation composition for treating or preventing fatigue, angiocardiopathy or inflammation or improving immunity
On the way.The alimentation composition can be food product, beverage, functional health care food, nutritional agents, food additives, nutrient formulation and dote on
Thing food.
Embodiment
Embodiment 1：The traditional preparation methods of cordyceps sinensis
As shown in figure 1, collect the fresh cordyceps militaris sporocarps of 50g, by it at 101 DEG C pasteurization 10 minutes.Then will be through
The cordyceps sinensis for crossing pasteurization is freeze-dried 12 hours at -50 DEG C.Collect and grind freeze-dried cordyceps sinensis.Obtain brown powder
End.
Adenosine content is analyzed by UPLC, as described in test example 6.Cordyceps sinensis powder includes 25mg adenosines/100g powder
End.
Embodiment 2：Prepare Cordyceps
As shown in Fig. 2 collecting the fresh cordyceps militaris sporocarps of 50g, mince to obtain cordyceps sinensis slurries in milling machine.To cordyceps sinensis slurries
Middle addition 500g water.Then (show) to be bleached with grey box in fig. 2 in two steps：(immersion) cordyceps sinensis slurry is handled at 30 DEG C
Liquid 3 hours (180 minutes), then further handles cordyceps sinensis slurries 30 minutes at 85 DEG C.Finally, cordyceps sinensis is starched at 101 DEG C
Liquid is sterilized 10 minutes, and frozen overnight is dried at -50 DEG C.Freeze-dried cordyceps sinensis is collected, and grinding obtains brown ceramic powder.
Adenosine content is analyzed by UPLC, as described in test example 6.Cordyceps sinensis powder includes 176mg adenosines/100g powder
End.
Embodiment 3：Prepare Cordyceps
As shown in figure 3, collecting the fresh cordyceps militaris sporocarps of 50g, mince to obtain cordyceps sinensis slurries in milling machine.To cordyceps sinensis slurries
Middle addition 500g water.Then (show) to be bleached with grey box in figure 3 in two steps：(immersion) cordyceps sinensis 3 is handled at 30 DEG C
Hour (180 minutes), cordyceps sinensis slurries are then further handled at 85 DEG C 30 minutes.Finally, by cordyceps sinensis slurries mistake at -50 DEG C
Night is freeze-dried.Freeze-dried cordyceps sinensis is collected, and grinding obtains brown ceramic powder.
Adenosine content is analyzed by UPLC, as described in test example 6.Cordyceps sinensis powder includes 205mg adenosines/100g powder
End.In embodiment 3, with embodiment 2 on the contrary, the cordyceps sinensis slurries after bleaching are non-sterilized.Sterilizing seems to have adenosine content
Negative effect.
Embodiment 4：Prepare Cordyceps
As shown in figure 4, collecting the fresh cordyceps militaris sporocarps of 50g, mince to obtain cordyceps sinensis slurries in milling machine.To cordyceps sinensis slurries
Middle addition 500g water.Bleaching is carried out in a step (being shown in Fig. 4 with grey box)：Cordyceps sinensis slurries 30 are handled at 85 DEG C
Minute.Finally cordyceps sinensis slurries are sterilized 10 minutes at 101 DEG C, and frozen overnight is dried at -50 DEG C.Collect freeze-dried
Cordyceps sinensis, and grind obtain brown ceramic powder.
Adenosine content is analyzed by UPLC, as described in test example 6.Cordyceps sinensis powder includes 109mg adenosines/100g powder
End.In example 4, with embodiment 2 and 3 on the contrary, being bleached using a step.The bleaching of one step seems to obtain more compared to the bleaching of two steps
Low adenosine content.
Embodiment 5：Prepare Cordyceps
As shown in figure 5, collecting the fresh cordyceps militaris sporocarps of 50g, mince to obtain cordyceps sinensis slurries in milling machine.To cordyceps sinensis slurries
Middle addition 500g water.Bleaching is carried out in a step (being shown in Figure 5 with grey box)：Cordyceps sinensis slurries are handled at 30 DEG C
180 minutes.Finally cordyceps sinensis slurries are sterilized 10 minutes at 101 DEG C, and frozen overnight is dried at -50 DEG C.Collect chilled
Dry cordyceps sinensis, and grinding obtains brown ceramic powder.
Adenosine content is analyzed by UPLC, as described in test example 6.Cordyceps sinensis powder includes 158mg adenosines/100g powder
End.In embodiment 5, with embodiment 4 on the contrary, gently being bleached using a step.Gentle bleaching seems to obtain compared to potent bleaching
Higher adenosine content.
Following table summarizes embodiment 1-5 master operation and compares the adenosine content of grinding Cordyceps.
| Embodiment and figure | Bleaching | Sterilizing | Adenosine (mg/100g) |
| 1 | Nothing | Have | 25 |
| 2 | Two steps are gentle and then potent | Have | 176.37 |
| 3 | Two steps are gentle and then potent | Nothing | 205 |
| 4 | One step, it is potent | Have | 108.73 |
| 5 | One step, gently | Have | 157.77 |
Test example 6：The method for analyzing adenosine content
With reference to the method in Chinese Pharmacopoeia, methanol is used under ultrasound condition: water (1: 1, volume/volume) extracts fine cordyceps sinensis
Extract powder 30 minutes, and UPLC- is passed through using C18 posts (for example, Acquity BEH C18,2.1*100mm, 1.7 μm)
PDA is quantified.The analysis uses isocratic method, is carried out at room temperature with the flow velocity of 1.0mL/ minutes.Mobile phase is water: methanol (95:
5, volume/volume).It is 259.4nm for quantitative absorbing wavelength.
Cordyceps is analyzed with Acquity UPLC-PDA (water generation company of the U.S. (Waters, USA)).UPLC's is detailed
Thin parameter is shown in following table.
This method is used to analyze the adenosine content in the Cordyceps described in embodiment 1 to 5.Fig. 6 is UPLC-
The example of PDA analysis gained Cordyceps chromatograms.
The concentration of adenosine is calculated according to the standard curve of the reference material formation by injecting different content.For example, injection
It is set forth below to lift reference material to obtain calibration curve.
| Reference material title | Concentration (X values) | Adenosine responds (Y value) |
| 1 μ g/mL hybrid standard things | 1μg/mL | 16415 |
| 2 μ g/mL hybrid standard things | 2μg/mL | 29055 |
| 4 μ g/mL hybrid standard things | 4μg/mL | 64063 |
| 8 μ g/mL hybrid standard things | 8μg/mL | 130661 |
| 15 μ g/mL hybrid standard things | 15μg/mL | 246860 |
| 30 μ g/mL hybrid standard things | 30μg/mL | 476448 |
| 50 μ g/mL hybrid standard things | 50μg/mL | 810322 |
According to the above results, adenosine calibration equation, such as Y=aX+b are obtained.Unknown sample is then injected into, is met with a response
Y value, and calculate the X values represented with μ g/mL.Adenosine content A in final sample can be represented according to following formula with mg/100g：
X such as the adenosine concentration (μ g/mL) calculated using calibration equation
M example weights (g)
50 dilution volumes (mL)
1000 conversion factor from μ g to mg
Claims (15)
1. a kind of method for preparing Cordyceps, comprises the following steps：
A) cordyceps sinensis is ground,
B) cordyceps sinensis is bleached,
C) cordyceps sinensis after the bleaching is dried,
Wherein bleaching handles the cordyceps sinensis 20 to 240 minutes at a temperature of being included in 27 DEG C to 90 DEG C in water.
2. according to the method described in claim 1, carried out 30 minutes to 200 points at a temperature of 30 DEG C to 85 DEG C wherein bleaching
Clock.
3. method according to claim 1 or 2, wherein bleaching is by soaking the worm at a temperature of 30 DEG C to 45 DEG C
What grass was completed for 120 minutes to 200 minutes.
4. according to the method described in claim 1, carried out 30 minutes to 60 minutes at a temperature of 70 DEG C to 90 DEG C wherein bleaching.
5. according to the method described in claim 1, wherein bleaching is accomplished by the following way：In 30 DEG C to 45 DEG C of temperature
Under the cordyceps sinensis is soaked in water 120 minutes to 200 minutes, then by the cordyceps sinensis be heated to 70 DEG C to 90 DEG C temperature and
Kept for 30 minutes to 60 minutes in water.
6. according to the method described in claim 1, wherein bleaching is accomplished by the following way：30 DEG C to 45 DEG C first
At a temperature of the cordyceps sinensis is soaked in water 120 minutes to 200 minutes, the temperature is increased to 70 in 5 to 15 minutes
DEG C to 90 DEG C of second temperature, and keep the second temperature 30 to 45 minutes.
7. method according to any one of claim 1 to 6, wherein the weight ratio of the water and cordyceps sinensis is 0.5:1 to 2:
1, it is therefore preferable to 1:1 to 1.5:1.
8. method according to any one of claim 1 to 7, includes between the blanching step and the drying steps
The step of being sterilized 10 to 15 minutes at a temperature of 98 DEG C to 101 DEG C.
9. method according to any one of claim 1 to 8, wherein the drying steps are by freeze-drying, baking oven
Dry or be dried in vacuo what is carried out.
10. method according to any one of claim 1 to 9, also includes other grinding after the drying steps
Step.
11. method according to any one of claim 1 to 10, wherein the cordyceps sinensis is Cordyceps militaris (Cordyceps
) or Cordyceps Militaris (Cordyceps sinensis) militaris.
12. Cordyceps prepared by a kind of method using according to any one of claim 1 to 11, wherein the worm
Careless extract includes 100mg-220mg adenosines/100g extracts.
13. a kind of alimentation composition, comprising the Cordyceps that can be obtained according to any one of claim 1 to 11 or according to
Cordyceps described in claim 12.
14. alimentation composition according to claim 13, wherein the alimentation composition is selected from food product, beverage, work(
Can health food, nutritional agents, food additives, nutrient formulation and pet food.
15. the Cordyceps that can be obtained according to any one of claim 1 to 11 or worm according to claim 12
Careless extract is used for the use for preparing the alimentation composition for treating or preventing fatigue, angiocardiopathy or inflammation or improving immunity
On the way.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2014/095955 WO2016106704A1 (en) | 2014-12-31 | 2014-12-31 | A process for preparing a cordyceps extract |
| CNPCT/CN2014/095955 | 2014-12-31 | ||
| PCT/EP2015/080757 WO2016107776A1 (en) | 2014-12-31 | 2015-12-21 | A process for preparing cordyceps extract |
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| Publication Number | Publication Date |
|---|---|
| CN107105736A true CN107105736A (en) | 2017-08-29 |
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| CN201580071335.5A Withdrawn CN107105736A (en) | 2014-12-31 | 2015-12-21 | The method for preparing Cordyceps |
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|---|---|
| CN (1) | CN107105736A (en) |
| WO (2) | WO2016106704A1 (en) |
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|---|---|---|---|---|
| CN112401290A (en) * | 2020-09-25 | 2021-02-26 | 山东省食品发酵工业研究设计院 | Preparation method of tobacco Chinese caterpillar fungus strips |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018133835A1 (en) * | 2017-01-20 | 2018-07-26 | National Institute Of Biological Sciences, Beijing | Nucleoside analogue regulating mammalian circadian rhythm |
| CN107475128A (en) * | 2017-09-08 | 2017-12-15 | 吉林省天祺蛹虫草繁育研究有限公司 | A kind of production method of selenium-rich cordyceps |
| CN112868705A (en) * | 2021-02-05 | 2021-06-01 | 山西省农业科学院食用菌研究所 | Radix seu herba Gei aleppici nutrient element and preparation process thereof |
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| WO2016107776A1 (en) | 2016-07-07 |
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