CN101883846A - 用于生成氮杂环丁酮的还原酶多肽 - Google Patents

用于生成氮杂环丁酮的还原酶多肽 Download PDF

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Publication number
CN101883846A
CN101883846A CN2008801185718A CN200880118571A CN101883846A CN 101883846 A CN101883846 A CN 101883846A CN 2008801185718 A CN2008801185718 A CN 2008801185718A CN 200880118571 A CN200880118571 A CN 200880118571A CN 101883846 A CN101883846 A CN 101883846A
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residue
polypeptide
ketoreductase
seq
amino acid
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奥诺拉托·坎帕皮阿诺
艾米丽·穆德弗
波蒂·博勒普
拉玛·沃兰德里
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Codexis Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biotechnology (AREA)
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  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
CN2008801185718A 2007-10-01 2008-10-01 用于生成氮杂环丁酮的还原酶多肽 Pending CN101883846A (zh)

Applications Claiming Priority (3)

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US97655507P 2007-10-01 2007-10-01
US60/976,555 2007-10-01
PCT/US2008/078513 WO2009046153A1 (en) 2007-10-01 2008-10-01 Ketoreductase polypeptides for the production of azetidinone

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US (14) US7883879B2 (enExample)
EP (1) EP2205727B1 (enExample)
JP (1) JP5646328B2 (enExample)
KR (1) KR20100065387A (enExample)
CN (1) CN101883846A (enExample)
ES (1) ES2547528T3 (enExample)
HU (1) HUE027683T2 (enExample)
IL (2) IL204379A (enExample)
SI (1) SI2205727T1 (enExample)
WO (1) WO2009046153A1 (enExample)

Cited By (8)

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CN103045504A (zh) * 2012-12-05 2013-04-17 浙江工业大学 微生物催化制备(2s, 3r)-2-苯甲酰氨甲基-3-羟基丁酸酯及菌株
CN104531627A (zh) * 2014-12-23 2015-04-22 浙江大学 羰基还原酶、工程菌及其应用
CN104846025A (zh) * 2015-03-31 2015-08-19 浙江大学 一种制备(2s,3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN107354179A (zh) * 2016-05-09 2017-11-17 上海医药工业研究院 一种制备含有羟基的手性化合物的方法
CN104342412B (zh) * 2013-08-09 2018-12-21 南京朗恩生物科技有限公司 用于生产(s)-4-氯-3-羟基丁酸乙酯的酮还原酶突变体
CN112941043A (zh) * 2021-05-17 2021-06-11 中国科学院天津工业生物技术研究所 羰基还原酶突变体及在制备手性β’-羟基-β-氨基酸酯中的应用
CN115057796A (zh) * 2015-12-01 2022-09-16 焦作健康元生物制品有限公司 光学活性的β-羟基酯类化合物的中间体
CN119570755A (zh) * 2025-01-07 2025-03-07 上海泓博尚奕药物技术有限公司 一种酮还原酶突变体及其在合成(r)-1-(2,6-二氯苯基)乙醇中的应用

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JP5362359B2 (ja) 2005-10-12 2013-12-11 モルフォシス アーゲー ヒトＣＤ３８に特異的な完全ヒトＨｕＣＡＬＧＯＬＤ由来の治療抗体の生成とプロファイリング
JP2010517574A (ja) 2007-02-08 2010-05-27 コデクシス， インコーポレイテッド ケトレダクターゼおよびその使用
CN101784669B (zh) 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
CN101855342B (zh) 2007-09-13 2013-07-10 科德克希思公司 用于还原苯乙酮的酮还原酶多肽
CN101889081B (zh) 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
SI2205727T1 (sl) 2007-10-01 2015-09-30 Codexis, Inc. Polipeptidi ketoreduktaze za izdelavo azetidinona
SI2329013T1 (sl) 2008-08-27 2016-03-31 Codexis, Inc. Polipeptidi ketoreduktaze za proizvodnjo 3-aril-3-hidroksipropanamina iz 3-aril-3-ketopropanamina
US8288141B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288131B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides and uses thereof
EP2329014B1 (en) 2008-08-29 2014-10-22 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
WO2011022548A2 (en) 2009-08-19 2011-02-24 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
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CN104053771B (zh) 2011-11-18 2016-11-09 科德克希思公司 用于制备羟基取代的氨基甲酸酯的生物催化剂
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CN103373934A (zh) * 2013-06-14 2013-10-30 苏州汇和药业有限公司 碳青霉烯和青霉烯类药物的手性中间体的催化合成方法
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CN105765592B (zh) 2013-09-27 2019-12-17 科德克希思公司 用于酶变体的自动筛选的方法、装置和系统
HUE053379T2 (hu) 2013-11-13 2021-06-28 Codexis Inc Génmódosított imin-reduktázok és eljárások keton és amin vegyületek reduktív aminálására
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CA2968275C (en) 2014-11-25 2023-09-12 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US10696953B2 (en) 2015-02-10 2020-06-30 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
MX387224B (es) 2015-02-12 2025-03-18 Imago Biosciences Inc Inhibidores de kdm1a para el tratamiento de enfermedades.
WO2017001907A1 (en) 2015-06-29 2017-01-05 Teva Pharmaceuticals International Gmbh Biocatalytic processes for the preparation of vilanterol
WO2018035249A1 (en) * 2016-08-16 2018-02-22 Imago Biosciences, Inc. Compositions and methods for producing stereoisomerically pure aminocyclopropanes
WO2018035259A1 (en) 2016-08-16 2018-02-22 Imago Biosciences, Inc. Methods and processes for the preparation of kdm1a inhibitors
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IL279260B2 (en) 2018-05-11 2024-10-01 Imago Biosciences Inc Kdm1a inhibitors for the treatment of disease
CN109295019B (zh) * 2018-10-29 2021-01-12 浙江大学 一种醇脱氢酶突变体及其应用
WO2020187718A1 (en) 2019-03-15 2020-09-24 Morphosys Ag Anti-cd38 antibodies and pharmaceutical compositions thereof for the treatment of autoantibody-mediated autoimmune disease
CN111378704A (zh) * 2020-03-23 2020-07-07 焦作健康元生物制品有限公司 一种酮还原酶生产4-aa中间体的方法
CN111363730A (zh) * 2020-03-23 2020-07-03 焦作健康元生物制品有限公司 一种用于生产4-aa的酮还原酶制备方法
CN114634957B (zh) * 2020-12-15 2024-08-16 苏州引航生物科技有限公司 一种生物催化合成4aa中间体的方法
CN115478057B (zh) * 2021-05-31 2025-04-04 尚科生物医药（上海）有限公司 一种用于制备(s)-3,4-二羟基-2’-氯苯乙醇的酮还原酶突变体
CN120981566A (zh) * 2023-01-12 2025-11-18 诺华股份有限公司 工程化酮还原酶多肽
CN119842647B (zh) * 2024-12-29 2025-07-22 苏州诺恩斯生物科技有限公司 一种用于合成度洛西汀中间体的脱氢酶突变体及其应用

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Cited By (10)

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CN103045504A (zh) * 2012-12-05 2013-04-17 浙江工业大学 微生物催化制备(2s, 3r)-2-苯甲酰氨甲基-3-羟基丁酸酯及菌株
CN104342412B (zh) * 2013-08-09 2018-12-21 南京朗恩生物科技有限公司 用于生产(s)-4-氯-3-羟基丁酸乙酯的酮还原酶突变体
CN104531627A (zh) * 2014-12-23 2015-04-22 浙江大学 羰基还原酶、工程菌及其应用
CN104846025A (zh) * 2015-03-31 2015-08-19 浙江大学 一种制备(2s,3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN104846025B (zh) * 2015-03-31 2018-06-01 浙江大学 一种制备(2s，3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN115057796A (zh) * 2015-12-01 2022-09-16 焦作健康元生物制品有限公司 光学活性的β-羟基酯类化合物的中间体
CN107354179A (zh) * 2016-05-09 2017-11-17 上海医药工业研究院 一种制备含有羟基的手性化合物的方法
CN112941043A (zh) * 2021-05-17 2021-06-11 中国科学院天津工业生物技术研究所 羰基还原酶突变体及在制备手性β’-羟基-β-氨基酸酯中的应用
CN112941043B (zh) * 2021-05-17 2021-09-10 中国科学院天津工业生物技术研究所 羰基还原酶突变体及在制备手性β’-羟基-β-氨基酸酯中的应用
CN119570755A (zh) * 2025-01-07 2025-03-07 上海泓博尚奕药物技术有限公司 一种酮还原酶突变体及其在合成(r)-1-(2,6-二氯苯基)乙醇中的应用

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