CN101863720B - Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof - Google Patents
Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof Download PDFInfo
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- CN101863720B CN101863720B CN201010204561A CN201010204561A CN101863720B CN 101863720 B CN101863720 B CN 101863720B CN 201010204561 A CN201010204561 A CN 201010204561A CN 201010204561 A CN201010204561 A CN 201010204561A CN 101863720 B CN101863720 B CN 101863720B
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Abstract
The invention discloses a microbial fertilizer for holding water and fixing nitrogen of soil and a preparation method thereof. The microbial fertilizer comprises an organism-based high polymer and an organic matter, wherein the organism-based high polymer is prepared from the following raw materials: 7 to 10 of acrylic acid, 1 to 4 of potassium hydroxide, 2 to 5 of starch, 1.5 to 8 of water, 29 to 97.5 of Bacillus mucilaginosus liquid and 0.17 to 0.2 of ammonium persulfate; and the organic matter comprises 10 to 20 of grass carbon, 20 to 40 of brown coal, 20 to 50 of bentonite and 30 to 50 of humus earth. The microbial fertilizer integrates phosphorus dissolving, potassium dissolving, nitrogen fixing and water holding, can reduce fertilizer consumption by 50 percent when used in the soil, keep the soil moist, loose and soft, improve the air permeability and organic matter content of the soil, increase the soil aggregate year by year to improve the soil, improve the comprehensive productivity of the soil and increase the yield per mu by 20 to 30 percent, and also can improve the content of starch, proteins and vitamins in fruits and vegetables when used in the soil for growing the fruits and vegetables.
Description
Technical field
The present invention relates to a kind of microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof, can be adaptable across agricultural, gardens, soil improvement, preserve moisture and fertility, the engineering of conceding the land to forestry and grass, defend and control sand.
Background technology
At present, the fertilizer that farm crop are used is chemical fertilizer such as inorganic fertilizer, compoiste fertilizer mostly, owing to use nitrogen, phosphorus, potassium three elements chemical fertilizer all the year round, has made the soil microorganisms eubiosis cause havoc.High-density insolubles deposition is solidified, soil compaction, and the preserve moisture and fertility ability drop, soil activation and organic content reduce, and the farm crop desired nutritional is supplied with imbalance, and many effective nutritive substances are suppressed.The chemical fertilizer mu consumption of China constantly increases, and the mu effect of increasing production is not obvious, and the chemical fertilizer waste is serious; The annual utilization rate of nitrogen fertilizer that uses of China is merely 30-35%, and phosphate fertilizer is merely 10-20%, and potash fertilizer is 35-50%; Compare with external chemical fertilizer average utilization 60%, have a long way to go.For this reason, people have used the fixed nitrogen technology, hope nitrogen fixing and slowly release in soil.But mostly existing fixed nitrogen technology is the vinelandii biological nitrogen fixation, and its nitrogen fixation effect is not obvious, has satisfied not the plant-growth needs, also wants chemical nitrogen to replenish.Slowly-releasing nitrogen technology be a coated with resin in the existing chemical fertilizer, and coating dissolves release then in soil, the initial release time that it has just delayed nitrogen, do not prolong time of releasing of nitrogen, and the loss of nitrogen is the same with common chemical fertilizer.Mostly the microbial fertilizer that uses at present is phosphor bacterium and potassium bacterium, bacterial strain through liquid submerged fermentation with amplify acquisition bacterium liquid step by step, can P and K decomposing, but can not economize on water by fixed nitrogen, effect is single.
At present, the deterioration in China along with physical environment, climate warming, arid, phreatic exhausted day by day, plant husbandry, greening, sand control all are faced with acid test, especially need the western dry of a kind of resisting drought saving water technology, particularly China.Mostly the resisting drought saving water technology of China is the engineering type at present, as: building anti seepage channel, sprinkling irrigation, drip irrigation etc., is exactly agrotype in addition, as: film coating technique, water cellar water storage etc.But more than various water-saving technologies on using, all have certain drawback, wherein engineering type water-saving technology quantities is big, investment is big, as does not have country or pool that to build be to be difficult to realize the waste that exists evaporation simultaneously and leak.Though the agrotype water-saving technology is practical, on the sand soil that leaks, still can causes the waste of water, and have pollution problem.At present up-to-date water-saving technology is to use the high molecular polymer water-holding agent, and it utilizes solution method or suspension method to obtain through polyreaction, and the resisting drought saving water effect is obvious, but can not P and K decomposing and fixed nitrogen, and effect is single, and cost is high.
Summary of the invention
The object of the invention is exactly to solve the problems referred to above that exist in the prior art, provides a kind of collected explanations or commentaries phosphorus, potassium decomposing, fixed nitrogen in one, saving water, resisting drought outstanding effect microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof.
For accomplishing the object of the invention, technical solution of the present invention is: a kind of microbial fertilizer for holding water and fixing nitrogen of soil, and it comprises bio-based high molecular polymer and organic matter; By weight calculating, the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7-10, Pottasium Hydroxide 1-4, starch 2-5, water 1.5-8, Bacillus mucilaginosus bacterium liquid 29-97.5 and ammonium persulphate 0.17-0.2; Organic matter comprises peat composed of rotten mosses 10-20, brown coal 20-40, wilkinite 20-50 and vegetable mould 30-50.
The making method of above-mentioned said microbial fertilizer for holding water and fixing nitrogen of soil, it may further comprise the steps:
(1) processes bacterium liquid to Bacillus mucilaginosus through fermentation culture; Ferment used substratum by following material by weight being mixed with: Na
2HPO
41.1-2.3, MgSO
41.3-1.7, potassium felspar sand 1-3, agar 17-20, K
2HPO
41.1-1.9, NaCl 2-5, glucose 13-21, CaCO
33-7 and water 23-70.
(2) vinylformic acid, Pottasium Hydroxide, starch, the water with above-mentioned said amount places the stirring in-tank mixing to stir; Fully after the neutralization; The ammonium persulphate that when temperature reaches 50-55 ℃, adds said amount; Insert in the biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds said amount then mixes, through the register controlled temperature 42-47 ℃ of following low-temperature atomizing polymerization; Obtain containing the bio-based high molecular polymer of glue peptone appearance bacillus, again through the peat composed of rotten mosses, brown coal, wilkinite and the vegetable mould that adds said amount after the liquid nitrogen cryogenics drying stir powder or particle.
The present invention mixes the Bacillus mucilaginosus microbial inoculum with the simple of high molecular polymer, but crosslinked together through polyreaction bacterium liquid that contains Bacillus mucilaginosus and neutralization solution, has guaranteed the activity of microorganism strains.
Product of the present invention can adsorb nitrogen and moisture, nitrogen and moisture can not volatilized very soon, but slowly discharge according to the needs of soil, prolongs the time of releasing of nitrogen, does not take off fertilizer, and nitrogen and efficiency of water application are high, have reduced waste, have practiced thrift fertilizer and water resources.The all right phosphorus decomposing of product of the present invention, potassium decomposing, and be adsorbed slow release, reduce chemical phosphorus, potassium fixing in soil, thereby reduce fertilizer amount, reduce pollution.Can decompose simultaneously several kinds of mineral elements confession plant utilization in the soil, comprehensive nutrition, lasting fertilizer effect.Collected explanations or commentaries phosphorus of the present invention, potassium decomposing, fixed nitrogen and water conservation use product of the present invention in one in soil, can reduce by 50% fertilizer amount; Simultaneously can keep soil moistening for a long time, soft, improve the ventilation property and the organic content of soil; Increase soil aggregate year by year; Play the effect of improving the soil, and the integrated production capacity of soil is improved, can make per mu yield improve 20-30%.It is used for planting the soil of gourd, fruit and vegetable, also can improve the content of starch in the gourd, fruit and vegetable, protein, VITAMINs, increases the melon and fruit sugariness.
Embodiment
Below in conjunction with specific embodiment the present invention is done further description:
Embodiment 1, and present embodiment comprises bio-based high molecular polymer and organic matter.By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7, Pottasium Hydroxide 4, starch 2, water 1.5, Bacillus mucilaginosus bacterium liquid 97.5 and ammonium persulphate 0.2; Organic matter comprises the peat composed of rotten mosses 20, brown coal 40, wilkinite 20 and vegetable mould 30.
Its making method is that it may further comprise the steps:
(1) processes bacterium liquid to Bacillus mucilaginosus through fermentation culture.Adopt the heat-shock method to handle Bacillus mucilaginosus, improve seed uniformity, shorten fermentation period to 27 hour, at first press Na
2HPO
41.1, MgSO
41.7, potassium felspar sand 1, agar 20, K
2HPO
41.9, NaCl 5, glucose 13, CaCO
37, the part by weight of water 23 places preparing culture medium in the fermentor tank, heats through steam then, when the jar temperature reaches 33-37 ℃, keeps tank pressure 07.0-1.4kg/cm2, inoculation glue peptone appearance bacterial strain of bacillus; Pay close attention to temperature and tank pressure and keep fermentation 20-24 hour, obtain the bacterium liquid that bacteria containing amount reaches 700,000,000/milliliter.
(2) vinylformic acid, Pottasium Hydroxide, starch, the water with above-mentioned said amount places the stirring in-tank mixing to stir; Fully after the neutralization; The ammonium persulphate that when temperature reaches 50-55 ℃, adds said amount; Get into biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds said amount then mixes, 42-47 ℃ of following low-temperature atomizing polymerization; Obtain containing the polyacrylic acid potassium of glue peptone appearance bacillus, again through the peat composed of rotten mosses, brown coal, wilkinite and the vegetable mould that adds said amount after the liquid nitrogen cryogenics drying stir powder or particle.
Embodiment 2, and present embodiment comprises bio-based high molecular polymer and organic matter.By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 8, Pottasium Hydroxide 2, starch 3, water 5, Bacillus mucilaginosus bacterium liquid 55 and ammonium persulphate 0.18; Organic matter comprises the peat composed of rotten mosses 15, brown coal 30, wilkinite 30 and vegetable mould 40.
Its making method is with embodiment 1.Just processing the used substratum of bacterium liquid to Bacillus mucilaginosus through fermentation culture is mixed with by following material: Na
2HPO
41.7, MgSO
41.5, potassium felspar sand 2, agar 18, K
2HPO
41.6, NaCl 3, glucose 15, CaCO
35, water 50.
Embodiment 3, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 10, Pottasium Hydroxide 2.5, starch 4, water 3, Bacillus mucilaginosus bacterium liquid 29 and ammonium persulphate 0.17; Organic matter comprises the peat composed of rotten mosses 10, brown coal 20, wilkinite 50 and vegetable mould 50.
Its making method is with embodiment 1.Just processing the used substratum of bacterium liquid to Bacillus mucilaginosus through fermentation culture is mixed with by following material: Na
2HPO
42, MgSO
41.3, potassium felspar sand 3, agar 17, K
2HPO
41.1, NaCl 2, glucose 21, CaCO
33, water 70.
Embodiment 4, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7.5, Pottasium Hydroxide 3, starch 5, water 8, Bacillus mucilaginosus bacterium liquid 40 and ammonium persulphate 0.19; Organic matter comprises the peat composed of rotten mosses 17.5, brown coal 35, wilkinite 40 and vegetable mould 35.
Its making method is with embodiment 1.Just processing the used substratum of bacterium liquid to Bacillus mucilaginosus through fermentation culture is mixed with by following material: Na
2HPO
42.3, MgSO
41.4, potassium felspar sand 2.5, agar 19, K
2HPO
41.8, NaCl4, glucose 17, CaCO
34, water 40.
Embodiment 5, and present embodiment comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 8.5, Pottasium Hydroxide 1, starch 3.5, water 6.5, Bacillus mucilaginosus bacterium liquid 75 and ammonium persulphate 0.185; Organic matter comprises the peat composed of rotten mosses 12.5, brown coal 25, wilkinite 35 and vegetable mould 45.
Its making method is with embodiment 1.Just processing the used substratum of bacterium liquid to Bacillus mucilaginosus through fermentation culture is mixed with by following material: Na
2HPO
41.5, MgSO
41.6, potassium felspar sand 1.5, agar 18.5, K
2HPO
41.4, NaCl3.5, glucose 19, CaCO
36, water 60.
Claims (9)
1. microbial fertilizer for holding water and fixing nitrogen of soil, it is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating, the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7-10, Pottasium Hydroxide 1-4, starch 2-5, water 1.5-8, Bacillus mucilaginosus bacterium liquid 29-97.5 and ammonium persulphate 0.17-0.2; Organic matter comprises peat composed of rotten mosses 10-20, brown coal 20-40, wilkinite 20-50 and vegetable mould 30-50.
2. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7, Pottasium Hydroxide 4, starch 2, water 1.5, Bacillus mucilaginosus bacterium liquid 97.5 and ammonium persulphate 0.2; Organic matter comprises the peat composed of rotten mosses 20, brown coal 40, wilkinite 20 and vegetable mould 30.
3. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 8, Pottasium Hydroxide 2, starch 3, water 5, Bacillus mucilaginosus bacterium liquid 55 and ammonium persulphate 0.18; Organic matter comprises the peat composed of rotten mosses 15, brown coal 30, wilkinite 30 and vegetable mould 40.
4. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 10, Pottasium Hydroxide 2.5, starch 4, water 3, Bacillus mucilaginosus bacterium liquid 29 and ammonium persulphate 0.17; Organic matter comprises the peat composed of rotten mosses 10, brown coal 20, wilkinite 50 and vegetable mould 50.
5. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 7.5, Pottasium Hydroxide 3, starch 5, water 8, Bacillus mucilaginosus bacterium liquid 40 and ammonium persulphate 0.19; Organic matter comprises the peat composed of rotten mosses 17.5, brown coal 35, wilkinite 40 and vegetable mould 35.
6. microbial fertilizer for holding water and fixing nitrogen of soil according to claim 1 is characterized in that: it comprises bio-based high molecular polymer and organic matter; By weight calculating: the bio-based high molecular polymer is processed by following raw material: vinylformic acid 8.5, Pottasium Hydroxide 1, starch 3.5, water 6.5, Bacillus mucilaginosus bacterium liquid 75 and ammonium persulphate 0.185; Organic matter comprises the peat composed of rotten mosses 12.5, brown coal 25, wilkinite 35 and vegetable mould 45.
7. the making method of each the described microbial fertilizer for holding water and fixing nitrogen of soil among the claim 1-6, it comprises that step (1) processes bacterium liquid to Bacillus mucilaginosus through fermentation culture; It is characterized in that: it comprises that also step (2) places the stirring in-tank mixing to stir in vinylformic acid, Pottasium Hydroxide, starch, the water of said amount; Fully after the neutralization; The ammonium persulphate that when temperature reaches 50-55 ℃, adds said amount; Insert in the biochemical polymerization reactor, the Bacillus mucilaginosus bacterium liquid that adds said amount then mixes, through the register controlled temperature 42-47 ℃ of following low-temperature atomizing polymerization; Obtain containing the bio-based high molecular polymer of glue peptone appearance bacillus, again through the peat composed of rotten mosses, brown coal, wilkinite and the vegetable mould that adds said amount after the liquid nitrogen cryogenics drying stir powder or particle.
8. the making method of microbial fertilizer for holding water and fixing nitrogen of soil according to claim 7, it is characterized in that: the method for processing bacterium liquid to Bacillus mucilaginosus through fermentation culture in the step (1) is: at first press Na
2HPO
41.1-2.3, MgSO
41.3-1.7, potassium felspar sand 1-3, agar 17-20, K
2HPO
41.1-1.9, NaCl 2-5, glucose 13-21, CaCO
3The part by weight of 3-7 and water 23-70 places preparing culture medium in the fermentor tank, heats through steam then, when the jar temperature reaches 33-37 ℃, keeps tank pressure 07.0-1.4kg/cm
2, inoculation glue peptone appearance bacterial strain of bacillus; Pay close attention to temperature and tank pressure and keep fermentation 20-24 hour, obtain bacterium liquid.
9. the making method of microbial fertilizer for holding water and fixing nitrogen of soil according to claim 8, it is characterized in that: the bacteria containing amount of said bacterium liquid reaches 700,000,000/milliliter.
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| CN201010204561A CN101863720B (en) | 2010-06-22 | 2010-06-22 | Microbial fertilizer for holding water and fixing nitrogen of soil and preparation method thereof |
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| CN101863720B true CN101863720B (en) | 2012-10-24 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515890B (en) * | 2012-01-05 | 2013-12-18 | 中国农业大学 | Composition for promoting nodulation and nitrogen fixation of leguminous plant and preparation method thereof |
| CN103787781A (en) * | 2014-02-27 | 2014-05-14 | 王东 | High-efficiency anti-seepage fertilizer and preparation method thereof |
| CN105130639A (en) * | 2015-08-25 | 2015-12-09 | 全椒林农生态农业科技有限公司 | Apocarya planting soil improver and soil improving method |
| CN105884427A (en) * | 2016-04-21 | 2016-08-24 | 张楚沁 | Improved acidified soil water-retention compound fertilizer and preparation method thereof |
| CN109956821B (en) * | 2019-03-27 | 2021-06-04 | 沈阳丰恒泰农业科技有限公司 | Preparation method of synergistic particles for enhancing microbial fertilizer |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1475467A (en) * | 2002-08-14 | 2004-02-18 | 吴晓丽 | Bamboo forest biological fertilizer |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2706727B2 (en) * | 1988-05-11 | 1998-01-28 | 三洋化成工業株式会社 | Soil water retention agent and water retention method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1475467A (en) * | 2002-08-14 | 2004-02-18 | 吴晓丽 | Bamboo forest biological fertilizer |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
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| Title |
|---|
| JP平1-285119A 1989.11.16 |
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