CN101855342B - 用于还原苯乙酮的酮还原酶多肽 - Google Patents

用于还原苯乙酮的酮还原酶多肽 Download PDF

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CN101855342B
CN101855342B CN2008801157703A CN200880115770A CN101855342B CN 101855342 B CN101855342 B CN 101855342B CN 2008801157703 A CN2008801157703 A CN 2008801157703A CN 200880115770 A CN200880115770 A CN 200880115770A CN 101855342 B CN101855342 B CN 101855342B
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residue
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CN101855342A (zh
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杰克·梁
史提芬尼·J·珍妮
艾米丽·穆德弗
沙琳·奇恩
约翰·M·格鲁伯
安克·克莱伯
古伽特·W·哈思曼
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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CN2008801157703A 2007-09-13 2008-09-13 用于还原苯乙酮的酮还原酶多肽 Active CN101855342B (zh)

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US97205807P 2007-09-13 2007-09-13
US60/972,058 2007-09-13
PCT/US2008/076333 WO2009036404A2 (en) 2007-09-13 2008-09-13 Ketoreductase polypeptides for the reduction of acetophenones

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CN101855342B true CN101855342B (zh) 2013-07-10

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EP (1) EP2198018B1 (https=)
JP (4) JP5973131B2 (https=)
KR (1) KR101586503B1 (https=)
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Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101784669B (zh) * 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
KR101586503B1 (ko) 2007-09-13 2016-01-18 코덱시스, 인코포레이티드 아세토페논의 환원을 위한 케토리덕타제 폴리펩티드
WO2009042984A1 (en) * 2007-09-28 2009-04-02 Codexis, Inc. Ketoreductase polypeptides and uses thereof
WO2009046153A1 (en) 2007-10-01 2009-04-09 Codexis, Inc. Ketoreductase polypeptides for the production of azetidinone
HUE026181T2 (en) 2008-08-27 2016-05-30 Codexis Inc Ketoreductase polypeptide for the preparation of 3-aryl-3-hydroxypropanamine from 3-aryl-3-ketopropanamine
US8288141B2 (en) 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
CN102186972B (zh) * 2008-08-29 2014-08-20 科德克希思公司 用于立体选择性生产(4s)-3-[(5s)-5-(4-氟苯基)-5-羟基戊酰基]-4-苯基-1，3-噁唑烷-2-酮的酮还原酶多肽
SG178456A1 (en) 2009-08-19 2012-04-27 Codexis Inc Ketoreductase polypeptides for the preparation of phenylephrine
CN102884178B (zh) 2009-12-08 2014-12-03 科德克希思公司 拉唑化合物的合成
WO2011140219A1 (en) 2010-05-04 2011-11-10 Codexis, Inc. Biocatalysts for ezetimibe synthesis
HUE037616T2 (hu) 2010-12-08 2018-09-28 Codexis Inc Biokatalizátorok és eljárások armodafinil szintéziséhez
US9102963B2 (en) 2011-04-13 2015-08-11 Codexis, Inc. Biocatalytic process for preparing eslicarbazepine and analogs thereof
US20150087832A1 (en) 2011-10-27 2015-03-26 Merck Sharp & Dohme, Corp. Process for making beta 3 agonists and intermediates
WO2013062878A1 (en) 2011-10-27 2013-05-02 Merck Sharp & Dohme Corp. Process for making beta 3 angonists and intermediates
EP2780448B1 (en) 2011-11-18 2019-09-11 Codexis, Inc. Biocatalysts for the preparation of hydroxy substituted carbamates
EP2847214B1 (en) 2012-05-11 2017-12-27 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN104471070B (zh) * 2012-05-17 2018-09-25 基因泰克公司 制备羟基化环戊并嘧啶化合物和其盐的方法
PL2968269T3 (pl) 2013-03-15 2020-03-31 Merck Sharp & Dohme Corp. Sposób przygotowywania agonistów beta-3 i produktów pośrednich
US10150959B2 (en) 2013-03-15 2018-12-11 Merck Sharp & Dohme Corp. Immobilized ketoreductases and process for making and using immobilized ketoreductase
ES2734209T3 (es) 2013-08-06 2019-12-04 Imago Biosciences Inc Inhibidores de KDM1A para el tratamiento de enfermedades
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants
JP6643987B2 (ja) 2013-11-13 2020-02-12 コデクシス， インコーポレイテッド ケトン化合物およびアミン化合物の還元的アミノ化のための操作されたイミンレダクターゼおよび方法
EP3081644B1 (en) * 2013-12-10 2019-03-27 Amano Enzyme Inc. Modified lipase and use thereof
CN104975048B (zh) * 2014-04-02 2018-07-24 中国科学院过程工程研究所 梅岭霉素合成基因簇中酮还原酶MeiF在手性芳香醇生产中的应用
EP3134519B1 (en) 2014-04-22 2018-06-06 c-LEcta GmbH Ketoreductases
DK3224354T3 (da) 2014-11-25 2021-03-01 Codexis Inc Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser
WO2016130412A1 (en) 2015-02-10 2016-08-18 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
PL3256218T3 (pl) 2015-02-12 2025-03-31 Imago Biosciences Inc. Inhibitor kdm1a i jego zastosowanie w terapii
CN104846025B (zh) * 2015-03-31 2018-06-01 浙江大学 一种制备(2s，3r)-2-苯甲酰氨甲基-3-羟基丁酸甲酯的方法
CN105906656B (zh) * 2016-05-17 2018-01-23 凯莱英医药集团（天津）股份有限公司 一种克唑替尼中间体的合成方法
CN106047950A (zh) * 2016-06-30 2016-10-26 尚科生物医药（上海）有限公司 一种(s)‑1‑(2,6‑二氯‑3‑氟苯基)乙醇的生物制备方法
US20220025424A1 (en) * 2016-08-16 2022-01-27 Imago Biosciences, Inc. Compositions and methods for producing stereoisomerically pure aminocyclopropanes
WO2018035259A1 (en) 2016-08-16 2018-02-22 Imago Biosciences, Inc. Methods and processes for the preparation of kdm1a inhibitors
CN106636248A (zh) * 2016-12-21 2017-05-10 浙江海洋大学 一种利用羰基还原酶制备克唑替尼中间体的方法
CA3061133A1 (en) 2017-04-27 2018-11-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
EP4410295A3 (en) 2017-06-06 2024-10-16 Urovant Sciences GmbH Use of vibegron to treat overactive bladder
CN109112166B (zh) * 2017-06-26 2023-08-15 弈柯莱生物科技(上海)股份有限公司 酶法制备替卡格雷中间体
EP3652328A1 (en) 2017-07-14 2020-05-20 c-LEcta GmbH Ketoreductases
GB201802383D0 (en) * 2018-02-14 2018-03-28 Givaudan Sa Process
CN108624603A (zh) * 2018-03-30 2018-10-09 河北省科学院生物研究所 一种(s)-羰基还原酶及其编码基因与应用
BR112021001066A2 (pt) 2018-05-11 2021-04-20 Imago Biosciences, Inc. composto, método de tratamento de uma doença mediada por kdm1a, método de tratamento de uma doença mediada por globina, composição farmacêutica; método de inibição de kdm1a, método para obter um efeito em um paciente, e método para inibir pelo menos uma função de kdm1a
CA3106124A1 (en) 2018-07-16 2020-01-23 Novartis Ag Chemical process for preparing phenylpiperidinyl indole derivatives
KR102940911B1 (ko) 2018-12-05 2026-03-17 유로반트 사이언시즈 게엠베하 과민성 방광 증상의 치료를 위한 비베그론
CN109706191B (zh) * 2019-01-21 2022-09-09 南京欧信医药技术有限公司 一种托莫西汀中间体的酶催化合成方法
US11396666B2 (en) 2019-04-19 2022-07-26 Api Corporation Method for producing (1R,3R)-3-(trifluoromethyl)cyclohexan-1-ol and intermediate thereof
CN110218718A (zh) * 2019-06-19 2019-09-10 南京趣酶生物科技有限公司 固定化酮还原酶突变体及其在制备奈必洛尔手性醇中间体及其类似物中的应用
MX2022003684A (es) * 2019-09-26 2022-04-25 Codexis Inc Polipeptidos y polinucleotidos de ceto-reductasa.
CN111235123B (zh) * 2020-03-27 2020-10-27 长兴制药股份有限公司 一种醇溶液高浓度耐受的羰基还原酶及其应用
CN111718965A (zh) * 2020-06-17 2020-09-29 宁波酶赛生物工程有限公司 一种(2s,3s)-2,3-丁二醇的制备方法
CN115478059B (zh) * 2021-05-31 2025-04-11 尚科生物医药（上海）有限公司 一种酮还原酶突变体及其应用
IL322082A (en) * 2023-01-12 2025-09-01 Novartis Ag Engineered ketoreductase polypeptides
CN120624386B (zh) * 2025-06-12 2025-12-09 亳州学院 一种醇脱氢酶突变体及其合成覆盆子酮的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4014573C1 (https=) 1990-05-07 1991-10-10 Forschungszentrum Juelich Gmbh, 5170 Juelich, De
US5225339A (en) 1992-02-26 1993-07-06 The Scripps Research Institute Lactobacillus kefir alcohol dehydrogenase
JP3155107B2 (ja) 1993-01-12 2001-04-09 ダイセル化学工業株式会社 光学活性４−ハロ−３−ヒドロキシ酪酸エステルの製造方法
US5491077A (en) 1994-07-20 1996-02-13 Merck & Co., Inc. Microbial method
US5427933A (en) 1994-07-20 1995-06-27 Merck & Co., Inc. Reduction of phenylalkyl ketones to the corresponding (S)-hydroxy derivatives using mucor hiemalis IFO 5834
JPH08336393A (ja) 1995-04-13 1996-12-24 Mitsubishi Chem Corp 光学活性なγ−置換−β−ヒドロキシ酪酸エステルの製造法
GB9512837D0 (en) 1995-06-23 1995-08-23 Zeneca Ltd reduction of ketone groups
DE19610984A1 (de) 1996-03-21 1997-09-25 Boehringer Mannheim Gmbh Alkohol-Dehydrogenase und deren Verwendung zur enzymatischen Herstellung chiraler Hydroxyverbindungen
US5891685A (en) 1996-06-03 1999-04-06 Mitsubishi Chemical Corporation Method for producing ester of (S)-γ-halogenated-β-hydroxybutyric acid
DE19812004A1 (de) 1998-03-19 1999-09-30 Forschungszentrum Juelich Gmbh Dehydrogenasen mit verbesserter NAD-Abhängigkeit, deren Herstellung und Verwendung
ES2207202T3 (es) 1998-04-30 2004-05-16 Kaneka Corporation Procedimiento para producir derivados de acido 6-cianometil-1, 3-dioxano-4-acetico.
DE19857302C2 (de) 1998-12-14 2000-10-26 Forschungszentrum Juelich Gmbh Verfahren zur enantioselektiven Reduktion von 3,5-Dioxocarbonsäuren, deren Salze und Ester
DE60018909T2 (de) 1999-12-03 2006-03-30 Kaneka Corp. Neue carbonyl-reduktase, gen davon und verfahren der anwendung derselben
DE10037101A1 (de) 2000-07-27 2002-02-07 Degussa Rekombinant hergestellte Enzyme mit verbesserter NAD(H)-Akzeptanz
US7202070B2 (en) 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
BR0208265A (pt) 2001-03-22 2004-03-02 Bristol Myers Squibb Co Redução estereosseletiva de acetofenona substituìda
DE10119274A1 (de) 2001-04-20 2002-10-31 Juelich Enzyme Products Gmbh Enzymatisches Verfahren zur enantioselektiven Reduktion von Ketoverbindungen
CA2517256C (en) 2003-02-26 2013-04-30 Sugen, Inc. Aminoheteroaryl compounds as protein kinase inhibitors
JP4213524B2 (ja) 2003-04-17 2009-01-21 ダイセル化学工業株式会社 新規なカルボニル還元酵素、その酵素をコードするｄｎａを含むポリヌクレオチド、その製造方法、およびこれを利用した光学活性アルコールの製造方法
JP2007502111A (ja) 2003-08-11 2007-02-08 コデクシス， インコーポレイテッド ４−置換３−ヒドロキシ酪酸誘導体およびビシナルなシアノ，ヒドロキシ置換カルボン酸エステルの生成のための酵素的プロセス
DE10345772A1 (de) 2003-10-01 2005-04-21 Basf Ag Verfahren zur Herstellung von 3-Methylamino-1-(thien-2-yl)-propan-1-ol
WO2005054491A1 (ja) 2003-12-02 2005-06-16 Mercian Corporation 光学活性テトラヒドロチオフェン誘導体の製造方法、および、光学活性テトラヒドロチオフェン-3-オールの晶析方法
JP4604524B2 (ja) * 2004-03-16 2011-01-05 味の素株式会社 変異型アルドラーゼ、並びにこれを用いた光学活性ｉｈｏｇ及び光学活性モナティンの製造方法
BR122020017756B1 (pt) 2004-08-26 2022-02-15 Pfizer Inc Uso de compostos de aminoeteroarila enantiomericamente puros na preparação de um medicamento para o tratamento de crescimento celular anormal em um mamífero
WO2006021885A1 (en) * 2004-08-26 2006-03-02 Pfizer Inc. Enantioselective biotransformation for preparation of protein tyrosine kinase inhibitor intermediates
ME01309B (me) 2004-08-26 2013-12-20 Pfizer Pirazolom supstituirani heteroarilni spojevi kao inhibitori proteinskih kinaza
BRPI0514687A (pt) 2004-08-26 2008-06-17 Pfizer compostos amino heteroarila como inibidores de proteìna tirosina cinase
AT501496B1 (de) * 2005-02-21 2007-03-15 Iep Gmbh Verfahren zur enantioselektiven enzymatischen reduktion von ketoverbindungen
US7393667B2 (en) 2005-05-31 2008-07-01 Bristol-Myers Squibb Company Stereoselective reduction process for the preparation of pyrrolotriazine compounds
AT502395B1 (de) * 2005-07-27 2007-03-15 Iep Gmbh Oxidoreduktasen zur stereoselektiven reduktion von ketoverbindungen
US7879585B2 (en) 2006-10-02 2011-02-01 Codexis, Inc. Ketoreductase enzymes and uses thereof
CN101627116B (zh) * 2007-02-08 2013-07-10 科德克希思公司 酮还原酶及其用途
CN101784669B (zh) * 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
KR101586503B1 (ko) 2007-09-13 2016-01-18 코덱시스, 인코포레이티드 아세토페논의 환원을 위한 케토리덕타제 폴리펩티드
WO2009042984A1 (en) * 2007-09-28 2009-04-02 Codexis, Inc. Ketoreductase polypeptides and uses thereof
WO2009046153A1 (en) * 2007-10-01 2009-04-09 Codexis, Inc. Ketoreductase polypeptides for the production of azetidinone
WO2010027710A2 (en) * 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
US8288141B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
CN102186972B (zh) * 2008-08-29 2014-08-20 科德克希思公司 用于立体选择性生产(4s)-3-[(5s)-5-(4-氟苯基)-5-羟基戊酰基]-4-苯基-1，3-噁唑烷-2-酮的酮还原酶多肽

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides

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US10927351B2 (en) 2021-02-23
US9951318B1 (en) 2018-04-24
US10100288B2 (en) 2018-10-16
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