CN101843654B - Chinese medicinal composition for treating skin wounds and preparation method thereof - Google Patents
Chinese medicinal composition for treating skin wounds and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal composition for treating skin wounds and a preparation method thereof. The Chinese medicinal composition is prepared from 1 to 5 weight parts of cockroach, 1 to 5 weight parts of asiatic pennywort herb, 1 to 5 weight parts of liquoric root and 1 to 5 weight parts of Sinkiang arnebia root which serve as active ingredients. The method comprises the following steps of: performing ethamol extraction on the raw materials such as the cockroach and the asiatic pennywort herb in a weight ratio to obtain mixed water-soluble extractum 1, wherein the concentration of the mixed water-soluble extractum 1 is equal to 0.5 to 2.5g of crude medicaments every ml; soaking the liquoric root with ammonia water, concentrating and drying the liquoric root under the reduced pressure to obtain a dry extractum 2; performing ethamol extraction on the Sinkiang arnebia root in ratio amount, concentrating filtrate to obtain dry extractum, extracting the dry extractum with petroleum ether, and concentrating the dry extractum until forming dry extractum 3; and adding the mixed water-soluble extractum 1, the dry extractum 2 and the dry extractum 3 into a matrix and auxiliary materials needed by various external formulations, and preparing the mixture to obtain the Chinese medicinal external preparation for treating the skin wound. The Chinese medicinal composition can be used for the skin wound caused by various physical factors or chemical factors, has obvious curative effect. Animal experiments prove that the Chinese medicinal composition can promote the skin wound to quickly heal, can inhibit scars after wound healing, and has the effective rate of over 95 percent.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, relate in particular to the Chinese medicine composition of treatment skin trauma.
Background technology
The normal skin of body receives the destruction and the invasion and attack of factors such as extraneous physics, chemistry, microorganism, if injure skin corium, regular meeting produces the cicatrix of skin tissue; The skin physiology structure is difficult to obtain intact recovery; Seriously cutaneous function and attractive in appearance has caused huge puzzlement for people's psychology, causes then the confidence of live and work is lacked; Especially in the burns unit field, it is outstanding that this problem more shows.
In the Chinese medicine preparation of existing treatment skin trauma; Though skin trauma is had better therapeutic effect like moist expose burn ointment, the new drop of rehabilitation etc.; But exist to a certain extent also such as can not resisting cicatrization simultaneously, or produce the defective of aspect such as allergy.Study and a kind ofly can promote skin healing, can eliminate the no anaphylactoid medicine that the trauma skin scar tissue forms again and have bigger medical value and social meaning.
Summary of the invention
The purpose of this invention is to provide a kind of can promote union of wounded skin and suppress wound healing after cicatrix generation Chinese medicine composition with and preparation method thereof.
The present invention selects Blatta seu periplaneta, Herba Centellae, Radix Arnebiae (Radix Lithospermi) and Radix Glycyrrhizae to make up, and the combination of these medicines produces drug synergism each other, can not only promote union of wounded skin, and can effectively suppress the hypertrophy of wound surface scar tissue.Wherein, Blatta seu periplaneta be Blattaria (
Blattaria) Blattidae (
Blattidae) Blatta seu periplaneta
Blatta orientalis, America Lian
Periplaneta americanaIn any dry or fresh adult polypide.Blatta seu periplaneta has the promoting blood circulation, removing blood stasis and relieving pain effect, and its water solublity extractum can promote cell proliferation and new granulation tissue to increase, and quickens the sick tissue repair of decreasing, and promotes angiogenesis, quickens slough and comes off, and repairs all kinds of ulcer and wound and wound surface rapidly.
Herba Centellae is the samphire Herba Centellae
Centella asiatica (L.) UrbanHerb or whole herb with root; Herba Centellae has clearing away heat-damp and promoting diuresis, subduing swelling and detoxicating effect; Asiaticoside and Herba Centellae extract contained in the Herba Centellae can be suppressed to fibrocellular division growth and fibroblastic collagen protein synthesis process, aspect skin trauma, are mainly used in the treatment hypertrophic cicatrix and promote wound healing.
The said Radix Arnebiae (Radix Lithospermi) of the present invention is meant the comfrey Radix Arnebiae (Radix Lithospermi)
Lithospermum erythrorhizon Sieb. et Zucc., the false Radix Arnebiae (Radix Lithospermi) in Xinjiang-Tibet
Arnebia euchroma (Royle) Johnst.Or Yunnan Radix Arnebiae (Radix Lithospermi)
Onosma paniculatum Bur. et FranchRoot; Radix Arnebiae (Radix Lithospermi) then has the effect of removing heat from blood and promoting blood circulation, heat-clearing and toxic substances removing; Can suppress capillary permeability, inhibitory action arranged, can protect wound surface, protect from infection, promote epithelial proliferation and reparation, the acceleration wound healing oozing out with edema; The department of dermatologry external is mainly used in treatment burn and skin surface skin infection, is used for moist macule, burn, eczema, erysipelas, carbuncle and ulcer and also has significant curative effect.
Radix Glycyrrhizae is a glycyrrhizic legume
Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. G. inflata Bat. or Glycyrrhiza glabra L.
G. glabra L.Dry root and rhizome, Radix Glycyrrhizae has the effect of heat-clearing and toxic substances removing, coordinating the actions of various ingredients in a prescription, is used for skin sore, cushion toxicity etc.; Glycyrrhizin has the broad-spectrum antivirus action, and the enoxolone of its hydrolysis has the effect of adrenocortical steroid, can obviously suppress chemical mediators such as mastocyte release tissue amine; Directly suppress complement activation; Reduce the CIC ELISA level, reduce the generation of anaphylaxis medium, therefore be applicable to the treatment of multiple allergic skin disease.
The consumption that the present invention treats each component of Chinese medicine composition of skin trauma all has better curative effect in the scope of following weight parts: Blatta seu periplaneta 1-5 part, Herba Centellae 1-5 part, Radix Glycyrrhizae 1-5 part, Radix Arnebiae (Radix Lithospermi) 1-5 part.
Wherein, preferred ingredients is: 1 part of Blatta seu periplaneta, 1 part of Herba Centellae, 1 part in Radix Glycyrrhizae, 1 part of Radix Arnebiae (Radix Lithospermi).
The conventional method that can adopt Chinese medicine preparation is prepared into the external preparation of any regular dosage form with Chinese medicine composition of the present invention, like liniment, ointment, liniment, gel, external suspensoid, patch, lotion, Emulsion, paste, tincture, aerosol etc.
The method for preparing of the active component of Chinese medicine composition of the present invention: the ethanol that materials of weight proportions Blatta seu periplaneta, Herba Centellae are doubly measured with raw material gross weight 6-16 30%-100% in (1) soaked 24 hours in 20 ℃～40 ℃; Filter; Filtrating is recycled to syrupy shape extractum, adds physiological saline solution, obtains lower aqueous layer; The lower aqueous solution filtering and concentrating obtains mixing water dissolubility extractum 1, and the every ml of its concentration is equivalent to the 0.5-2.5g of crude drug in whole; (2) licorice piece is soaked with the ammonia that is equivalent to licorice raw material 10-14 times of weight, said ammonia ammon amount is 0.5%-1.5%, the dry extractum 2 that gets of concentrating under reduced pressure; (3) Radix Arnebiae (Radix Lithospermi) is added the ethanol of the 85%-95% be equivalent to Radix Arnebiae (Radix Lithospermi) 14-22 times of weight, 20 ℃ of-60 ℃ of reflux, extract,, filtrating concentrate dry extract, with 6 times of amounts of dry extract reuse medical material weight petroleum ether extraction, merge petroleum ether and be evaporated to dry extract 3 mutually.
Above-mentioned mixing water dissolubility extractum 1, dry extract 2 and dry extract 3 these three active sites are joined in the different Chinese medicine exterior-applied formulations of the preparation required various substrate and adjuvant, be prepared into Chinese medicine for external application, like microemulsion liniment, ointment, tincture, patch etc.
Chinese medicine composition of the present invention has blood circulation promoting and blood stasis dispelling, clearing away heat-damp and promoting diuresis, subduing swelling and detoxicating effect, can be used for the skin trauma that various physics or chemical factor cause, and therapeutic effect is remarkable.Confirm through animal experiment, can promote union of wounded skin rapidly, and can suppress the generation of cicatrix behind the wound healing.The skin that physics such as burn and scald, incision, golden wound and chemical factor are caused is damaged has the good curing effect, the generation that impels tissue regeneration and suppress wound healing later stage cicatrix, and its effective percentage can reach more than 95%.
Description of drawings
Fig. 1 is skin trauma model empty group and the 1st day photo of administration group postoperative in the pharmacodynamic experiment;
Fig. 2 is skin trauma model empty group and the 3rd day photo of administration group postoperative in the pharmacodynamic experiment;
Fig. 3 is skin trauma model empty group and the 8th day photo of administration group postoperative in the pharmacodynamic experiment;
Fig. 4 is skin trauma model empty group and the 15th day photo of administration group postoperative in the pharmacodynamic experiment.
The specific embodiment
Embodiment 1The microemulsion formulation of preparation Chinese medicine composition of the present invention
With 50 ℃ of vacuum dryings, mixing behind the pulverize separately adds the ethanol of 85% mass concentration of 14 times of amounts of raw material gross weight with raw material of Chinese medicine periplaneta americana polypide 1000g and Herba Centellae 1000g; Soaked 24 hours under the room temperature; Filter, repeat 3 times, merging filtrate also is recycled to heavy-gravity syrupy shape extractum; 20 ℃ of-60 ℃ of dissolved in distilled water with 2 times of amounts of Blatta seu periplaneta polypide raw material weight; After stirring is fully dissolved it; Standing demix; Remove the upper strata insoluble matter, the gained filtrate decompression concentrated and obtains 2000ml mixing water dissolubility extractum 1 after lower aqueous solution filtered, and every ml mixing water dissolubility extractum 1 is equivalent to the 1g of raw material.
Raw medicinal material Glycyrrhiza inflata Bat. 1000g with 50 ℃ of vacuum dryings, is pulverized, and the ammon amount that adds Radix Glycyrrhizae 12 times of weight is that the ammonia of 1 % soaked 24 hours, filter, filtrate decompression concentrate dry extract 2.
Raw material Radix Arnebiae (Radix Lithospermi) 1000g with 50 ℃ of vacuum dryings, is pulverized, add 90% ethanol of 10 times of amounts of weight; 30 ℃ of reflux, extract, 2 hours are filtered, and filtering residue repeats to extract 1 time in the same manner; Merging filtrate, and be evaporated to dry extract, 6 times of petroleum ether extractiones of this dry extract reuse medical material weight; Merge petroleum ether extraction liquid, be evaporated to dry extract 3.
Get each 1000g of Blatta seu periplaneta, Herba Centellae, Radix Glycyrrhizae and Radix Arnebiae (Radix Lithospermi), obtain Blatta seu periplaneta polypide mixing water dissolubility extractum 1000ml, Radix Glycyrrhizae extractum 145g, Radix Arnebiae (Radix Lithospermi) dry extract 41g according to above-mentioned steps respectively.
Mixing water dissolubility extractum 1 2000ml
Radix Glycyrrhizae dry extractum 2 145g
Radix Arnebiae (Radix Lithospermi) dry extract 3 41g
Tween-80 668ml
Glycerin 334ml
Isopropyl myristate 130ml
The preparation of pastille microemulsion formulation:
1. the preparation of blended emulsifier: Polyoxyethylene Sorbitol Fatty Acid Esters (Tween-80) 668ml and glycerin 334ml mix, and stir, and at room temperature leave standstill and promptly get blended emulsifier in 5 minutes.
2. the preparation of oil phase material: get the 41g Radix Arnebiae (Radix Lithospermi) dry extract and be dissolved in the 130ml isopropyl myristate, sonic oscillation fully dissolves, and qualitative filter paper filters, and filtrating is oil phase.
3. the preparation of water material: get 1000ml mixing water dissolubility extractum, dissolving 145g Radix Glycyrrhizae dry extractum sonic oscillation fully dissolves in this extractum, and qualitative filter paper filters, and filtrating is water.
4. oil phase material is joined in the blended emulsifier neutralizing tank, stir, make oil phase material fully emulsified, left standstill 5 minutes.
5. the water material is slowly joined in the above-mentioned oily newborn mixture, till the limit edged is stirred to and adds, continues again to be stirred to and make it form uniform emulsion droplet, leave standstill 5 minutes can fill and obtain the pastille microemulsion.
Embodiment 2The ointment formulation of preparation Chinese medicine composition of the present invention
With 50 ℃ of vacuum dryings, mixing behind the pulverize separately adds the ethanol of 85% volumetric concentration of 14 times of amounts of raw material gross weight with raw material of Chinese medicine periplaneta americana polypide 1000g and Herba Centellae 5000g; Soaked 24 hours under the room temperature, filter, repeat 3 times; Merging filtrate also is recycled to heavy-gravity syrupy shape extractum, with 50 ℃ of physiological saline solutions of 1 times of amount of raw material weight, stir it is fully dissolved after; Standing demix is obtained lower aqueous solution, filters; Filtrate decompression concentrates and obtains 3000ml mixing water dissolubility extractum 1, and every ml mixing water dissolubility extractum 1 is equivalent to the 2g of raw material.
Raw medicinal material Glycyrrhiza inflata Bat. 1000g with 50 ℃ of vacuum dryings, is pulverized, and the ammon amount that adds Radix Glycyrrhizae 12 times of weight is that the ammonia of 1 % soaked 24 hours, filter, filtrate decompression concentrate 145g dry extract 2.
Raw material Radix Arnebiae (Radix Lithospermi) 1000g with 50 ℃ of vacuum dryings, is pulverized, add 90% ethanol of 10 times of amounts of weight; 30 ℃ of reflux, extract, 2 hours are filtered, and filtering residue repeats to extract 1 time in the same manner; Merging filtrate, and be evaporated to dry extract, 6 times of petroleum ether extractiones of this dry extract reuse medical material weight; Merge petroleum ether extraction liquid, be evaporated to 41g dry extract 3.
The ointment prescription is following:
Mixing water dissolubility extractum 1000ml
Radix Glycyrrhizae dry extractum 145g
Radix Arnebiae (Radix Lithospermi) dry extract 41g
Stearic acid 234g
Glyceryl monostearate 68g
Liquid paraffin 118g
White vaseline 19g
Lanoline 98g
Triethanolamine 8.8g
The method for preparing of ointment formulation:
1. get oil-phase component stearic acid, glyceryl monostearate, liquid paraffin, white vaseline and lanoline in beaker; Place and be heated to 70 ℃ of left and right sides mixed meltings in the water-bath; And then with the Radix Arnebiae (Radix Lithospermi) dry extract adding wherein, stirring and dissolving, 60 ℃ of insulations obtain about oil phase.
2. triethanolamine, Radix Glycyrrhizae dry extractum and mixing water dissolubility extractum are put in the beaker,, made it fully dissolve mixing, obtain water in also being heated to about 60 ℃ in the water-bath.
3. under 60 ℃ of isothermys, while stirring the water material is slowly joined in the oil phase material with the thread shape, in water-bath, continue to keep constant temperature and be stirred to brown semi-solid, promptly get O/W type emulsion-type substrate.
Embodiment 3The another kind of ointment formulation of Chinese medicine composition of the present invention
With 50 ℃ of vacuum dryings, mixing behind the pulverize separately adds the ethanol of 85% volumetric concentration of 14 times of amounts of raw material gross weight with raw material of Chinese medicine periplaneta americana polypide 5000g and Herba Centellae 1000g; Soaked 24 hours under the room temperature, filter, repeat 3 times; Merging filtrate also is recycled to heavy-gravity syrupy shape extractum, with 50 ℃ of physiological saline solutions of 0.5 times of amount of raw material weight, stir it is fully dissolved after; Standing demix is obtained lower aqueous solution, filters; Filtrate decompression concentrates and obtains 3000ml mixing water dissolubility extractum 1, and every ml mixing water dissolubility extractum 1 is equivalent to the 2g of raw material.
Raw medicinal material Glycyrrhiza inflata Bat. 1000g with 50 ℃ of vacuum dryings, is pulverized, and the ammon amount that adds Radix Glycyrrhizae 12 times of weight is that the ammonia of 1 % soaked 24 hours, filter, filtrate decompression concentrate 145g dry extract 2.
Raw material Radix Arnebiae (Radix Lithospermi) 1000g with 50 ℃ of vacuum dryings, is pulverized, add 90% ethanol of 10 times of amounts of weight; 30 ℃ of reflux, extract, 2 hours are filtered, and filtering residue repeats to extract 1 time in the same manner; Merging filtrate, and be evaporated to dry extract, 6 times of petroleum ether extractiones of this dry extract reuse medical material weight; Merge petroleum ether extraction liquid, concentrating under reduced pressure gets 41g dry extract 3.
The ointment prescription is following:
Mixing water dissolubility extractum 1 3000ml
Radix Glycyrrhizae dry extractum 2 145g
Radix Arnebiae (Radix Lithospermi) dry extract 3 41g
Stearic acid 234g
Glyceryl monostearate 68g
Liquid paraffin 118g
White vaseline 19g
Lanoline 98g
Triethanolamine 8.8g
All the other steps of ointment formulation are with embodiment 2.
Embodiment 4 pharmacodynamics tests
Get 4 SD rats, male and female are not limit, and ether inhalation anesthesia directly cuts off the chaeta of the rat ridge left and right sides and the buttocks left and right sides with shears, and the depilation area is about 5cm
2, with 75% alcohol disinfecting, the excision diameter is the circular holostrome skin of 0.5cm in the ridge both sides respectively after the normal saline cleaning, drying, and skin of buttock remains intact.
Set up the skin trauma model of the same area and the degree of depth.One group is the blank group, and another group is the administration group.The administration group is smeared embodiment 1 prepared medicine every day, each 0.5ml, and every separated 8h smears once, and administration time is fixed.Successive administration 15 days takes off scar to whole wound healings, observes the wound healing situation of blank control group and administration group every day and keeps the score, takes pictures, and writes down the time that two groups of wound healings take off scar.Last administration (the 15th day) back 72h gets the skin repair situation that skin sample is done tissue slice observation blank group and administration group of being tried.
(1) gross examination of skeletal muscle of pharmacodynamic experiment and standards of grading
0 minute: wound did not have redness, and color and luster is normal, and blood effusion is not obvious, and the wound surface cicatrix is very thin, did not have contracture basically, and healing is good;
1 minute: the slightly red or swelling of wound, a small amount of blood effusion is arranged, a small amount of outmoded blood crusts is arranged, cicatrix is thinner, slightly contracture;
2 minutes: wound redness, blood effusion is many, and blood crusts is obvious, and cicatrix is obvious, and it is poor to heal;
3 minutes: wound infection, cicatrix was heavier, delayed healing.
(2) histopathological examination
After the gross examination of skeletal muscle this group rat is anaesthetized respectively, in wound outside 0.5cm do at place fusiform incision with the 11# sharp knife with wound and outside 0.5cm place skin, subcutaneous tissue holostrome cut, dissociate and organize; Fixing 24 hours of 10% neutral formalin solution, FFPE, section (thick 5um); Inflammatory cell in the wound of the left and right sides, mononuclear phagocyte are observed in HE dyeing, optical microscope down; Fibroblast, epidermis cell, granulation tissue and cambium.
(3) statistical analysis
Observe standards of grading according to general form, the experimental data of collecting is handled through self paired t-test.
(4) result
Laboratory animal is carried out the cardinal principle perusal, and the scar of laboratory animal control sides and administration side all comes off after 15 days.1 day both sides wound of the postoperative mat woven of fine bamboo strips red and swollen, courage and uprightness ooze than aspect there was no significant difference, not statistically significant; The 3rd, 8 days both sides wounds of postoperative have statistical significance (P ﹤ 0.05) at aspect significant differences such as redness, blood crusts, contracture and cicatrixs, and administration group wound does not have outmoded blood crusts, do not have redness, and contracture and cicatrix are slight; The blank control group wound circumference has outmoded blood crusts, and slight red and swollen, contracture and cicatrix are apparent in view.
The 15th day both sides scar of postoperative all comes off, and sees Fig. 1, Fig. 2, Fig. 3, Fig. 4.Wherein, Fig. 1 is the 1st day photo of postoperative, and photo shows that the control sides blood effusion is more, and administration side blood effusion is less.Fig. 2 is the 3rd day photo of postoperative, the control sides edge of wound have outmoded blood crusts, contracture obviously, big, the wound surface of wound area is lower than that normal skin is a lot of on every side; Administration side wound recovers better, wound area dwindles, wound surface flushes with normal skin on every side basically.Fig. 3 is the 8th day photo of postoperative, shows among the figure: the control sides edge of wound has obvious blood crusts, wound area bigger; Administration side wound recovers better, wound area is less, wound surface is dry.Fig. 4 is the 15th day photo of postoperative, shows among the figure, and control sides and administration side scar all come off, and control sides has than clear scar and suture impression; Administration side cicatrix is more very thin, and the suture impression is not obvious.Experimental result shows that this Chinese medicine composition can promote the healing of skin trauma preferably, and highly significant ground reduces the generation of skin trauma cicatrix.
Claims (8)
1. external medicine composition of treating skin trauma is characterized in that: said Chinese medicine composition is by Blatta seu periplaneta 1-5 part of weight portion, Herba Centellae 1-5 part, and Radix Glycyrrhizae 1-5 part, Radix Arnebiae (Radix Lithospermi) 1-5 part forms as the active component feedstock production.
2. Chinese medicine composition as claimed in claim 1 is characterized in that: said Chinese medicine composition active component raw material consist of 1 part of Blatta seu periplaneta, 1 part of Herba Centellae, 1 part in Radix Glycyrrhizae, 1 part of Radix Arnebiae (Radix Lithospermi).
3. comprise according to claim 1 or claim 2 Chinese medicine composition as the Chinese medicine for external application of active component.
4. Chinese medicine for external application as claimed in claim 3 is characterized in that: said preparation is a microemulsion.
5. Chinese medicine for external application as claimed in claim 3 is characterized in that: said preparation is an ointment.
6. the method for preparing of Chinese medicine for external application as claimed in claim 3; It is characterized in that comprising the following step: materials of weight proportions Blatta seu periplaneta, Herba Centellae are doubly measured with raw material gross weight 6-16 the ethanol of 30%-100% in 20 ℃～40 ℃ immersions 24 hours in (1), filter, and filtrating is recycled to syrupy shape extractum; Add physiological saline solution; Obtain lower aqueous layer, the lower aqueous solution filtering and concentrating obtains mixing water dissolubility extractum 1, and the every ml of its concentration is equivalent to the 0.5-2.5g of crude drug in whole; (2) the licorice raw material decoction pieces with proportional quantity soaks with the ammonia that is equivalent to Radix Glycyrrhizae 10-14 times of weight, and said ammonia ammon amount is 0.5%-1.5%, the dry extractum 2 that gets of concentrating under reduced pressure; (3) add the ethanol of the 85%-95% of Radix Arnebiae (Radix Lithospermi) 14-22 times of weight amount, 20 ℃ of-60 ℃ of reflux, extract,, filtrating concentrate dry extract, with 6 times of amounts of dry extract reuse medical material weight petroleum ether extraction, merge petroleum ether and be evaporated to dry extract 3 mutually; Mixing water dissolubility extractum 1, dry extract 2 and dry extract 3 are joined in various the exterior-applied formulations required substrate and adjuvant, prepare the required Chinese medicine for external application that is used to treat skin trauma.
7. the method for preparing of Chinese medicine microemulsion as claimed in claim 4; It is characterized in that comprising the following step: materials of weight proportions Blatta seu periplaneta, Herba Centellae are doubly measured with raw material gross weight 6-16 the ethanol of 30%-100% in 20 ℃～40 ℃ immersions 24 hours in (1), filter, and filtrating is recycled to syrupy shape extractum; Add physiological saline solution; Obtain lower aqueous layer, the lower aqueous solution filtering and concentrating obtains mixing water dissolubility extractum 1, and the every ml of its concentration is equivalent to the 0.5-2.5g of crude drug in whole; (2) ammonia with licorice piece raw material 10-14 times of weight soaks, and said ammonia ammon amount is 0.5%-1.5%, the dry dry extract 2 that gets of concentrating under reduced pressure; (3) add the ethanol of the 85%-95% of Radix Arnebiae (Radix Lithospermi) 14-22 times of weight amount, 20 ℃ of-60 ℃ of reflux, extract,, filtrating concentrate dry extract, with 6 times of amounts of dry extract reuse medical material weight petroleum ether extraction, merge petroleum ether and be evaporated to dry extract 3 mutually; (4) Tween-80, glycerin are mixed and made into emulsifying agent; With processing oil phase material in the dry extract 3 adding isopropyl myristates; Dry extract 2 is dissolved in preparation water material in the mixing water dissolubility extractum 1, oil phase material and water material are joined respectively in the emulsifying agent, be prepared into microemulsion.
8. the method for preparing of Chinese medicine ointment as claimed in claim 5; It is characterized in that comprising the following step: materials of weight proportions Blatta seu periplaneta, Herba Centellae are doubly measured with raw material gross weight 6-16 the ethanol of 30%-100% in 20 ℃～40 ℃ immersions 24 hours in (1), filter, and filtrating is recycled to syrupy shape extractum; Add physiological saline solution; Obtain lower aqueous layer, the lower aqueous solution filtering and concentrating obtains mixing water dissolubility extractum 1, and the every ml of its concentration is equivalent to the 0.5-2.5g of crude drug in whole; (2) ammonia with licorice piece raw material 10-14 times of weight soaks, and said ammonia ammon amount is 0.5%-1.5%, the dry dry extract 2 that gets of concentrating under reduced pressure; (3) add the ethanol of the 85%-95% of Radix Arnebiae (Radix Lithospermi) 14-22 times of weight amount, 20 ℃ of-60 ℃ of reflux, extract,, filtrating concentrate dry extract, with 6 times of amounts of dry extract reuse medical material weight petroleum ether extraction, merge petroleum ether and be evaporated to dry extract 3 mutually; (4) get oil-phase component stearic acid, glyceryl monostearate, liquid paraffin, white vaseline and lanoline mixed melting; And then wherein with Radix Arnebiae (Radix Lithospermi) dry extract 3 addings; Radix Glycyrrhizae dry extractum 2 and mixing water dissolubility extractum 1 are dissolved in the triethanolamine; The water material is slowly joined in the oil phase material with the thread shape, stir and be prepared into ointment.
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| CN106074990A (en) * | 2016-06-30 | 2016-11-09 | 大理美莎农业科技开发有限公司 | A kind of reparation spraying treating wound and processing method thereof |
| CN109432158A (en) * | 2018-12-18 | 2019-03-08 | 沈阳药科大学 | A kind of Chinese medicine composition, its external application latex and its preparation method and application |
| CN112121146B (en) * | 2019-06-25 | 2021-08-06 | 山东瑞安药业有限公司 | A topical gel for treating skin wound, and its preparation method |
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