CN101829172A - Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition - Google Patents
Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition Download PDFInfo
- Publication number
- CN101829172A CN101829172A CN201010176053A CN201010176053A CN101829172A CN 101829172 A CN101829172 A CN 101829172A CN 201010176053 A CN201010176053 A CN 201010176053A CN 201010176053 A CN201010176053 A CN 201010176053A CN 101829172 A CN101829172 A CN 101829172A
- Authority
- CN
- China
- Prior art keywords
- ganoderma
- lucidum
- mycoplasm
- medicinal mycoplasm
- radix ginseng
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a ganoderma-lucidum medicinal mycoplasm, which is prepared by a method comprising the following steps: pulverizeing ginseng, adding water to moisten, and sterilizing to obtain the fermentation substrate; activating the ganoderma lucidum strain, and carrying out enlarged culture to obtain the fermentation strain; and inoculating the fermentation strain in the fermentation substrate, and carrying out bidirectional solid fermentation at 24-28 DEG C for 10-20 days under a lucifugal condition. The invention provides a pharmaceutical preparation which comprises the ganoderma-lucidum medicinal mycoplasm and a medicinal carrier. The invention provides a pharmaceutical composition which comprises the ganoderma-lucidum medicinal mycoplasm, pharmacological active substances and a medicinal carrier. The invention also provides a food composition which comprises the ganoderma-lucidum medicinal mycoplasm and nutriologically acceptable food additives. The ganoderma-lucidum medicinal mycoplasm has the active constituents in both ginseng and ganoderma lucidum, and thus, has favorable effects of health care and medicinal function.
Description
Technical field
The present invention relates to technical field of bioengineering, relate in particular to a kind of ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food compositions.
Background technology
Radix Ginseng contains saponins, polysaccharide, volatilization wet goods various active composition, has that invigorating primordial QI, multiple arteries and veins are taken off admittedly, an effect of invigorating the spleen to benefit the lung, promoting the production of body fluid and inducing sedation of the mind, is typical medicine-food two-purpose plant.But present Radix Ginseng converted products as Radix Ginseng Rubra, Radix Ginseng, active ginseng, Radix Ginseng tea, Radix Ginseng soda pop etc., is Radix Ginseng to be soaked or grind the back to process with other edibles or pharmaceutically useful material mostly, only contains the effective ingredient of Radix Ginseng.
Ganoderma is delicious flavour not only, also contain bioactive substances such as polysaccharide, aminoacid, vitamin and inorganic salt, have multiple efficacies such as enhancing human body immunity power, antitumor, antiviral, slow down aging, blood fat reducing, angiocardiopathy preventing, have higher health care and medical value.
China's bacteriology can Zhuan Yi teach proposed in 1998 medicinal fungi on medicinal mycoplasma, carry out the theory of solid fermentation and method (research of PTS Auricularia Sophorae electuary. Chinese Pharmaceutical Journal .1998,33 (5).P273～275)。During the two-way solid fermentation of fungus and medical material, medical material provides growth needed nutrition for fungus, and the enzyme of fungus also can be to the exert an influence change of the composition that causes medical material of medical material.Prior art discloses multiple medicinal fungi and multiple medical material carries out two-way solid fermentation, is that 200510041362.6 Chinese patent literature discloses medicinal fungi and Radix Tripterygii Wilfordii are carried out two-way solid fermentation as application number, with the method for the poison of separating Radix Tripterygii Wilfordii; Application number is that the Chinese patent literature of 200810023423.X discloses and a kind of medicinal fungi and Semen Strychni carried out the method for two-way solid fermentation, has realized the poison that falls of Semen Strychni is deposited effect.
The inventor considers, conventional ginseng and Ganoderma are carried out two-way solid fermentation, makes the ganoderma-lucidum medicinal mycoplasm that obtains both contain the effective ingredient of conventional ginseng, contains the effective ingredient of Ganoderma again, has good health care and medicinal efficacy.
Summary of the invention
In view of this, technical problem to be solved by this invention is to provide a kind of ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food compositions, increase the effective ingredient of fungus when making it have the conventional ginseng effective ingredient, improve its medicinal and health-care effect.
The invention provides a kind of ganoderma-lucidum medicinal mycoplasm, prepare by following method:
With adding water-wet after the Radix Ginseng pulverizing, obtain fermentation substrate after the sterilization;
With carrying out amplification culture after the Ganderma lucidum strain activation, obtain fermented bacterium;
Described fermented bacterium is inoculated in the fermentation substrate, under 24 ℃～28 ℃, the condition of lucifuge, carried out two-way solid fermentation 10 days～20 days.
The present invention also provides a kind of pharmaceutical preparation, comprises described ganoderma-lucidum medicinal mycoplasm of technique scheme and pharmaceutically useful carrier, and the mass ratio of described ganoderma-lucidum medicinal mycoplasm and described pharmaceutically useful carrier is 1: 0.1～3.
Preferably, described pharmaceutically useful carrier is starch, dextrin, cyclamate, Icing Sugar or Polyethylene Glycol.
Preferably, described preparation is powder, granule, tablet, capsule, pill, drop pill, soft capsule or oral liquid.
The present invention also provides a kind of pharmaceutical composition, comprises the described ganoderma-lucidum medicinal mycoplasm of technique scheme, pharmacological active substance and pharmaceutically useful carrier.
Preferably, described pharmacological active substance is one or more in Lentinus Edodes, Semen Nelumbinis, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Polygalae and the above-mentioned substance extract.
The present invention also provides a kind of food compositions, comprises that described ganoderma-lucidum medicinal mycoplasm of technique scheme and threpsology go up acceptable food additive.
Preferably, described food additive is one or more in starch, dextrin, cyclamate, Icing Sugar and the Polyethylene Glycol.
Compared with prior art, the invention provides a kind of ganoderma-lucidum medicinal mycoplasm, this ganoderma-lucidum medicinal mycoplasm is the product that Ganoderma and Radix Ginseng carry out two-way solid fermentation, not only contains the effective ingredient of conventional ginseng, and contains the effective ingredient of Ganoderma.During the fermentation, Radix Ginseng provides growth essential nutrient substance for Ganoderma, and chemical reaction can take place in enzyme in the Ganoderma and the chemical constituent in the Radix Ginseng, make growth have the Radix Ginseng of Ganoderma mycelium to have new nature,taste and action, show than better health care of conventional ginseng, Ganoderma and both simple additions and medicinal efficacy.Ganoderma-lucidum medicinal mycoplasm provided by the invention can become pharmaceutical preparation with pharmaceutically useful preparing carriers, uses as Cure for insomnia, adjusting immunity, antineoplastic medicine; Also can form composite medicine with other pharmacological components, use as medicine, performance invigorating primordial QI, multiple arteries and veins take off admittedly, invigorating the spleen to benefit the lung, the effect of promoting the production of body fluid and inducing sedation of the mind and the drug effect of other pharmacological components; Ganoderma-lucidum medicinal mycoplasm provided by the invention can also be prepared into the food with health care with food additive, has that invigorating primordial QI, multiple arteries and veins are taken off admittedly, an effect of invigorating the spleen to benefit the lung, promoting the production of body fluid and inducing sedation of the mind, can strengthen the body constitution of sub-health population.
The specific embodiment
The alleged two-way solid-fermented technique of the present invention is meant the technology that fungus (Ganoderma) is fermented on the solid fermentation substrate that Chinese crude drug (Radix Ginseng) is formed, during the fermentation, the effect of fungus and Chinese crude drug is two-way, promptly Chinese crude drug provides nutrient substance such as the required C of thalli growth, N as fermentation substrate for fungus on the one hand, enzyme in the fungus and the chemical constituent in the Chinese crude drug react on the other hand, make Chinese crude drug have new flavor efficacy.
The alleged ganoderma-lucidum medicinal mycoplasm of the present invention is meant the mycoplasma that Ganoderma grows and ferments and obtain on the solid fermentation substrate that Radix Ginseng is formed.The product of Ganoderma behind solid fermentation comprises that thalline is a secondary metabolites, and these products may be present in the somatic cells, also may be secreted into the extracellular and enter in the fermentation substrate Radix Ginseng; Simultaneously, because the Radix Ginseng after a large amount of mycelium of Ganoderma growth and the fermentation can't separate, therefore the material that obtains after the fermentation is referred to as ganoderma-lucidum medicinal mycoplasm.
The alleged fermentation substrate of the present invention refer to provide conk the material of essential composition, mainly be Radix Ginseng, also can be the substrate of forming by Radix Ginseng and agricultural byproducts.
The alleged conventional ginseng of the present invention is meant unprocessed, and especially the Radix Ginseng of handling without two-way solid-fermented technique mainly is to do to shine Radix Ginseng or fresh ginseng.
The invention provides a kind of ganoderma-lucidum medicinal mycoplasm, prepare by following method:
With adding water-wet after the Radix Ginseng pulverizing, obtain fermentation substrate after the sterilization;
With carrying out amplification culture after the Ganderma lucidum strain activation, obtain fermented bacterium;
Described fermented bacterium is inoculated in the fermentation substrate, under 24 ℃～28 ℃, the condition of lucifuge, carried out two-way solid fermentation 10 days～20 days.
The present invention is the fermentation substrate of Ganoderma with the Radix Ginseng, makes Radix Ginseng and Ganoderma carry out two-way solid fermentation, prepares ganoderma-lucidum medicinal mycoplasm.During the fermentation, Radix Ginseng provides necessary nutrient substance for the Ganoderma growth, and the enzyme in the Ganoderma acts on the chemical constituent in the Radix Ginseng, makes the effective ingredient that had both contained Radix Ginseng in the ganoderma-lucidum medicinal mycoplasm that obtains, the effective ingredient that contains Ganoderma again has medicinal preferably and health-care effect.
The present invention preferably uses Radix Ginseng or fresh ginseng, is preferably free from insect pests, does not have the Radix Ginseng that goes mouldy.
After pulverizing with other modes with fresh ginseng section or with Radix Ginseng's pulverizing or with Radix Ginseng, add water-wet.
The purpose that adds water is for fungi fermentation provides essential Free water, owing to be solid fermentation, amount of water can not be too much simultaneously.Among the present invention, the weight that adds water is preferably 50%～150% of Radix Ginseng dry weight, and more preferably 100%～150%.In order to make moisture fully be penetrated into Radix Ginseng inside, wetting time is preferably 6h～12h.
With the Radix Ginseng after moistening sterilization, it is standby promptly to can be used as fermentation substrate.Among the present invention, the pressure when sterilizing is preferably 0.1MPa～0.14MPa, and sterilising temp is preferably 121 ℃～126 ℃, and sterilization time is preferably 30min～60min.
The present invention uses Ganoderma to ferment, and the present invention does not have particular restriction to the source of described Ganderma lucidum strain, and the used strain of the present invention is the Ganderma lucidum strain that Changchun University of Traditional Chinese Medicine's medicinal fungi institute is preserved.
The present invention at first Ganderma lucidum strain is activated and amplification culture after, obtain fermented bacterium, fermented bacterium is inoculated in the fermentation substrate ferments then.The concrete steps of Ganderma lucidum strain being carried out amplification culture comprise that Ganderma lucidum strain is inoculated into potato dextrose agar slant medium (PDA slant medium) to be cultivated 5 days～10 days for last 24 ℃～28 ℃, obtain mycelium, then mycelium are carried out amplification culture.
The method of the mycelium that obtains being carried out amplification culture can be the liquid medium shake-flask culture, also can cultivate for solid medium, and the present invention preferably carries out the liquid medium shake-flask culture, preferably includes following steps:
Configuration contains the glucose of 2wt%, the peptone of 0.5wt%, the yeast powder of 0.1wt%, the KH of 0.15wt%
2PO
4And 0.1wt%MgSO
4Culture fluid, sterilization back is standby;
The mycelium that cuts after the activation inserts in the culture fluid, and shaken cultivation is 5 days～7 days under 24 ℃～28 ℃, the condition of 120r/min～170rmin, obtains fermented bacterium.
Pollute for fear of producing, mycelium inserts the operation of culture fluid preferably to carry out under aseptic condition.
Fermented bacterium is inserted in the Radix Ginseng fermentation substrate, under 24 ℃～28 ℃, the condition of lucifuge, carried out two-way solid fermentation 10 days～20 days, obtain ganoderma-lucidum medicinal mycoplasm.When carrying out two-way solid fermentation, fermentation temperature is preferably 26 ℃～28 ℃, and fermentation time is preferably 10 days～and 12 days.
During the fermentation, Radix Ginseng provides its growth essential nutrient substance for Ganoderma, and the enzyme in the Ganoderma reacts with the chemical constituent in the Radix Ginseng simultaneously, makes the new nature,taste and action of Radix Ginseng acquisition by the content that produces new chemical constituent or change original composition.The product of Ganoderma behind solid fermentation may be present in the somatic cells, also may be secreted into the extracellular enters in the fermentation substrate Radix Ginseng, because the Radix Ginseng after a large amount of mycelium of Ganoderma growth and the fermentation can't separate, therefore the ganoderma-lucidum medicinal mycoplasm that the obtains effective ingredient that both contained Radix Ginseng also contains the effective ingredient of Ganoderma.
After ganoderma-lucidum medicinal mycoplasm oven dry pulverizing, to examine under a microscope, the ganoderma-lucidum medicinal mycoplasm powder has the feature of Radix Ginseng and Ganoderma simultaneously:
Calcium oxalate cluster crystal diameter 20 μ m～68 μ m, corner angle are sharp-pointed;
The resin canal fragment contains block yellow secretions in a tubular form;
Cork cell's class is square, and wall is thin, wavy bending;
Many reticulate patterns of conduit or scalariform;
Closely colourless, elongated, the mutual travelling expenses of mycelium interweave, and diameter is 2 μ m～5 μ m, has tabula.
According to the method that Chinese Pharmacopoeia provides described ganoderma-lucidum medicinal mycoplasm is carried out thin-layer chromatographic analysis, the chromatograph that obtains is compared with Radix Ginseng control medicinal material chromatograph, at ginsenoside Rb
1, ginsenoside Re, ginsenoside Rg
1The relevant position on show and the point of same color as seen, to contain ginsenoside Rb in the described ganoderma-lucidum medicinal mycoplasm
1, ginsenoside Re, ginsenoside Rg
1
With ultraviolet-uisible spectrophotometer described ganoderma-lucidum medicinal mycoplasm is carried out analyzing and testing, the content that records total polysaccharides, total saponins and total protein in the described ganoderma-lucidum medicinal mycoplasm is respectively 20wt%, 3.7wt% and 5.5wt%, do not have significant difference in the content of total saponins and the conventional ginseng, but the content of total polysaccharides and total protein all is higher than the content of corresponding composition in the conventional ginseng.
Described ganoderma-lucidum medicinal mycoplasm is configured to be subjected to reagent thing solution, white mice is carried out toxicity test, fail to measure the half lethal dose of ganoderma-lucidum medicinal mycoplasm, experimental results show that described ganoderma-lucidum medicinal mycoplasm, and can promote the growth of white mice not only to the white mice avirulence.
To be configured to solution after described ganoderma-lucidum medicinal mycoplasm and the Tween 80 mediation, white mice is carried out the effect experiment, the ganoderma-lucidum medicinal mycoplasm of experimental result proof the present invention preparation can increase the length of one's sleep, raising sleep incidence rate, the shortening sleep latent time of mice, and effect all is better than the mixture of Radix Ginseng, Ganoderma, Radix Ginseng and Ganoderma.
This shows that described Ganoderma medicinal fungal substance contains the effective ingredient of Radix Ginseng and Ganoderma, has favorable effects.Simultaneously, Ganoderma medicinal fungal substance toxicity provided by the invention is extremely low, can calm the nerves, calmness Cure for insomnia.
The present invention also provides a kind of pharmaceutical preparation, comprises described ganoderma-lucidum medicinal mycoplasm of technique scheme and pharmaceutically useful carrier.Wherein, the mass ratio of described ganoderma-lucidum medicinal mycoplasm and described pharmaceutically useful carrier is 1: 0.1～3.
Among the present invention, described pharmaceutically useful carrier includes but not limited to adhesive, filler, disintegrating agent, the lubricant in the tablet; Wine in the medicine pill, vinegar, medicine juice etc.; Substrate part in semi-solid preparation ointment, the cream; Antiseptic in the liquid preparation, antioxidant, correctives, aromatic, cosolvent, emulsifying agent, solubilizing agent, osmotic pressure regulator, coloring agent etc. are preferably starch, dextrin, cyclamate, Icing Sugar or Polyethylene Glycol.
The present invention does not have particular restriction to the form of described pharmaceutical preparation, is preferably the pharmaceutical preparation of oral forms such as powder, granule, tablet, capsule, pill, drop pill, soft capsule or oral liquid.
Pharmaceutical preparation provided by the invention has calmness, calms the nerves, the effect of hypnotic, can be used for the treatment of insomnia.
The present invention also provides a kind of pharmaceutical composition, comprises the described ganoderma-lucidum medicinal mycoplasm of technique scheme, other pharmacological active substancies and pharmaceutically useful carrier.
Among the present invention, other pharmacological active substancies can be for except understanding toxigenous material with the ginseng compatibility, as other pharmacological active substancies beyond the anti-Rhizoma et radix veratri (Radix Rhizoma Veratri) etc., include but not limited in Lentinus Edodes, Semen Nelumbinis, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Polygalae and the above-mentioned substance extract one or more.
Pharmaceutical composition provided by the invention as the effect of Lentinus Edodes, Radix Angelicae Sinensis etc., can be used for the treatment of insomnia, improve immunity, antitumor except having the ganoderma-lucidum medicinal mycoplasm calmness, calm the nerves, the effect of hypnotic, also having other pharmacological active substancies.
The present invention also provides a kind of food compositions, comprises that described ganoderma-lucidum medicinal mycoplasm of technique scheme and threpsology go up acceptable additive.
Described threpsology goes up acceptable additive and is mainly food additive, includes but not limited to antiseptic such as sodium benzoate, potassium sorbate, lactic acid; Antioxidant such as vitamin C, isoascorbic acid; Coloring agent such as natural pigment; Thickening agent such as dextrin, pure glue or stabilizing agent; Bulking agent such as sodium bicarbonate; Sweeting agent such as cyclamate, Icing Sugar is preferably in starch, dextrin, cyclamate, Icing Sugar and the Polyethylene Glycol one or more.
Food compositions provided by the invention can also comprise other nutritional labelings such as protein.
It is edible that food compositions provided by the invention can be used as health product, health invigorating, raising immunity, hypnotic.
Compared with prior art, handle the effective ingredient that the ganoderma-lucidum medicinal mycoplasm that obtains contains Radix Ginseng and Ganoderma, have than Radix Ginseng or Ganoderma or better health care of both simple additions and medicinal efficacy by method provided by the invention.Experiment shows that in the ganoderma-lucidum medicinal mycoplasm provided by the invention, the content and the conventional ginseng of total saponins do not have significant difference, but the content of total polysaccharides and total protein is all than the content height of respective substance in the conventional ginseng.Simultaneously, described medicinal mycoplasm has the distinctive fragrance of fungus, can cover the bitterness of Radix Ginseng, improves the mouthfeel of medicinal mycoplasm, makes that the Radix Ginseng product is easier to be accepted by masses.In addition, medicinal mycoplasm provided by the invention can be prepared into preparation or form composite medicine with other pharmacological components with pharmaceutically useful material, when using, have that invigorating primordial QI, multiple arteries and veins are taken off admittedly as medicine, invigorating the spleen to benefit the lung, the effect of promoting the production of body fluid and inducing sedation of the mind and the drug effect of other pharmacological components; Medicinal mycoplasm provided by the invention can also be prepared into the food with health care with food additive, can strengthen the body constitution of sub-health population.
In order further to understand the present invention, ganoderma-lucidum medicinal mycoplasm provided by the invention, medicine preparation, composite medicine and food compositions are described in detail below in conjunction with embodiment.
Embodiment 1
The ganoderma strain capable that Changchun University of Traditional Chinese Medicine medicinal fungi institute is preserved is seeded on the PDA slant medium, cultivate 27 ℃ ± 1 ℃ the time, cultivates that to obtain mycelium in 7 days standby;
Making contains 2wt% glucose, 0.5wt% peptone, 0.1wt% yeast powder, 0.15wt%KH
2PO
4, and 0.1wt%MgSO
4Bacteria culture fluid.Get the 100mL bacteria culture fluid and pack in the 250mL triangular flask, 0.1MPa, 121 ℃ of sterilization 30min.The mycelium that cuts 4 0.5cm * 0.5cm in sterilizing room inserts in the bacteria culture fluid of triangular flask, and shaken cultivation obtained containing the seed liquor of mycelium pellet after 5 days under 28 ℃, the condition of 150r/min;
Get the 50g free from insect pests, do not have the Radix Ginseng go mouldy and pulverized 60 mesh sieves, add 50g water-wet 12h after, the Radix Ginseng that obtains is packed in the 500mL triangular flask, 0.1MPa behind the sterilization 60min, insert the 50mL seed liquor, dark culturing is 10 days in the time of 28 ℃, obtains ganoderma-lucidum medicinal mycoplasm.
With ganoderma-lucidum medicinal mycoplasm oven dry, pulverizing, obtain having the yellow-white powder of Ganoderma fragrance;
Described powder is differentiated at microscopically visible powder has following feature: mycelium is closely colourless, elongated, diameter 2 μ m～5 μ m, visible tabula, travelling expenses interweave mutually; Calcium oxalate cluster crystal diameter 20 μ m～68 μ m, corner angle are sharp-pointed; The resin canal fragment contains block yellow secretions in a tubular form; Cork cell's class is square, and wall is thin, wavy bending; Many reticulate patterns of conduit or scalariform.
According to the method that provides in the Chinese Pharmacopoeia described powder is carried out thin-layer chromatographic analysis, the chromatograph that obtains is compared with Radix Ginseng control medicinal material chromatograph, at ginsenoside Rb
1, ginsenoside Re, ginsenoside Rg
1The relevant position on show and to illustrate the point of same color and to contain ginsenoside Rb in the described powder
1, ginsenoside Re, ginsenoside Rg
1
Utilize the TU1810 ultraviolet-uisible spectrophotometer of Beijing Puxi General Instrument Co., Ltd that described powder is analyzed, the content that records total polysaccharides, total saponins and total protein in the powder is respectively 20wt%, 3.7wt% and 5.5wt%.
Embodiment 2
The medicinal mycoplasm toxicity test
2.1 laboratory animal and grouping
40 Kunming kind white mice that Changchun High-technology Medical Animal Experiment Research Center provides, male and female half and half, body weight 18g～22g.
2.2 experimental apparatus and reagent
Electronic scale, little mouse cage and 1mL irrigation stomach device;
Get the ganoderma-lucidum medicinal mycoplasm powder of 100g embodiment 1 preparation, after being in harmonious proportion with the 0.2g Tween 80, add the 400g distilled water be configured to mass concentration be 25% be subjected to reagent thing solution;
0.2g Tween 80 and 400g distilled water are configured to contrast solution.
2.3 preliminary experiment
Get 20 white mice, male and female half and half, water 16h is can't help in fasting, is divided into sample sets and matched group;
That gives that the mice lavage of sample sets gives the 7.5g/kg body weight every day is subjected to reagent thing solution, continuous irrigation 7 days, the life of finding white mice in order, the no phenomena of mortality, maximum tolerated dose that hence one can see that are 7.5g/kg.
2.4 acute toxicity testing
Get 20 sample sets white mice, male and female half and half, water 16h is can't help in fasting, is divided into sample sets and matched group; Early, middle and late three times the sample sets mice lavage is subjected to reagent thing solution with 40mL/kg every day; Every day early, middle and late three times to the contrast solution of matched group mice lavage with 40mL/kg; Continuous irrigation stomach 14 days is irritated normal diet between gastric phase;
Behind each gastric infusion, observe toxic reaction immediately, observed continuously 14 days, the sample sets white mice is movable after the discovery administration reduces, and recovers normal behind the 24h; And the outward appearance of white mice, the mental status, appetite, defecation number of times and color thereof, do not had obvious difference with matched group by hair, the colour of skin, breathing, secretions etc., and do not see the toxic reaction relevant with medicine, there is not dead generation.
Before irritating stomach, write down the body weight of sample sets and matched group white mice respectively, the body weight of sample sets and matched group white mice during record irritate stomach the 3rd day, the 7th day, the 11st day and the 15th day respectively, adopt DAS2.0 statistics software that each data is carried out Treatment Analysis, adopt the t check, inspection level α=0.05, the results are shown in Table 1, respectively organize the variation of white mice body weight in the toxicity test that table 1 provides for the embodiment of the invention.
Respectively organize the variation of white mice body weight in the toxicity test that table 1 embodiment of the invention provides
As shown in Table 1, ganoderma-lucidum medicinal mycoplasm provided by the invention has facilitation to the body weight gain of mice.
Irritate stomach and in the time of the 15th day white mice is put to death with the cervical vertebra dislocation method that causes death, white mice is dissected internal organs such as perusal white mice liver, kidney, no abnormal change; Respectively organize the ratio of white mice liver and body weight and the ratio of kidney and body weight, adopt DAS2.0 statistics software that each data is carried out Treatment Analysis, adopt the t check, inspection level α=0.05, the results are shown in Table 2, respectively organize the ratio (liver body ratio) of white mice liver and body weight and the ratio (kidney body ratio) of kidney and body weight in the toxicity test that table 2 provides for the embodiment of the invention.
Respectively organize white mice liver body ratio and kidney body ratio in the toxicity test that table 2 embodiment of the invention provides
As shown in Table 2, irritated liver body ratio and kidney body ratio and the matched group white mice no significant difference of the white mice of stomach ganoderma-lucidum medicinal mycoplasm, as seen, ganoderma-lucidum medicinal mycoplasm does not have the overt toxicity reaction to the internal organs of white mice.
By above-mentioned toxicity test as can be known, ganoderma-lucidum medicinal mycoplasm not only toxicity is extremely low, can also promote the growth promoter of white mice.
Embodiment 3
Medicinal mycoplasm has the effect experiment that improves sleep
3.1 laboratory animal
Male ICR mouse, body weight 18g～26g, Jilin University's Experimental Animal Center provides.
3.2 reagent
Get the ganoderma-lucidum medicinal mycoplasm powder of embodiment 1 preparation, adding distil water be made into mass concentration be 25% be subjected to reagent thing solution;
With the Ganoderma pulverizing that Jilin Province gold space pharmaceutical developments company limited provides, it is 25% Ganoderma solution that adding distil water is made into mass concentration;
The Radix Ginseng pulverizing that Jilin Province gold space pharmaceutical developments company limited is provided, it is 25% Radix Ginseng solution that distilled water is made into mass concentration;
With Ganoderma and the common pulverizing of Radix Ginseng, it is 25% Ganoderma Radix Ginseng mixed liquor that adding distil water is made into mass concentration;
Pentobarbital sodium (AR), China Medicine (Group) Shanghai Chemical Reagent Co., produces.
3.3 direct sleep experiments
Choose 50 mices, be divided into 5 groups at random, be respectively Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and matched group;
By the dosage of 1.0g/kg body weight respectively with Radix Ginseng solution, Ganoderma solution, ginseng and lucid ganoderma mixed liquor, be subjected to reagent thing solution and distilled water to irritate stomach to give each mice in Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and the matched group, irritate stomach after 30 days, observe the sleep incidence rate of respectively organizing mice, find respectively to organize mice and all do not have the generation of sleep phenomenon.During observation, the hypnotic instrument righting reflex loss is a criterion, when mice places supine position, can not right in 1 minute, promptly thinks righting reflex loss to enter sleep; Righting reflex recovers to be the animal awakening; Righting reflex loss is to being the animal sleep time between convalescent period.
3.4 prolong the pentobarbital sodium experiment length of one's sleep
Choose 50 mices, be divided into 5 groups at random, be respectively Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and matched group;
By the dosage of 1.0g/kg body weight respectively with Radix Ginseng solution, Ganoderma solution, ginseng and lucid ganoderma mixed liquor, be subjected to reagent thing solution and distilled water to irritate stomach to give each mice in Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and the matched group, irritated stomach 30 days.The last filling behind the stomach behind the 15min, each organizes the pentobarbital sodium of mouse peritoneal injection 50mg/kg body weight, injection volume is 0.2mL/20g, with the righting reflex loss is index, observes the length of one's sleep and writes down time for falling asleep and the awakening time, adopts DAS2.0 statistics software that each data is carried out Treatment Analysis, adopt the t check, inspection level α=0.05 the results are shown in Table 3, respectively organizes the length of one's sleep of mice during the effect that table 3 provides for the embodiment of the invention is tested.
Respectively organize the length of one's sleep of mice in the effect experiment that table 3 embodiment of the invention provides
As shown in Table 3, ganoderma-lucidum medicinal mycoplasm can increase the length of one's sleep of mice, and effect is better than the mixture of Radix Ginseng, Ganoderma, Radix Ginseng and Ganoderma.
3.5 pentobarbital sodium sub-threshold lull dosage experiment
Choose 50 mices, be divided into 5 groups at random, be respectively Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and matched group;
By the dosage of 1.0g/kg body weight respectively with Radix Ginseng solution, Ganoderma solution, ginseng and lucid ganoderma mixed liquor, be subjected to reagent thing solution and distilled water to irritate stomach to give each mice in Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and the matched group, irritated stomach 30 days.The last filling behind the stomach behind the 15min, each organizes the maximum subliminal hypnosis dosage of pentobarbital sodium of mouse peritoneal injection 25mg/kg body weight, observe and the interior sleeping number of mice of record 30min, righting reflex loss 1min is above for sleeping, the results are shown in Table 4, respectively organize the sleep incidence rate of mice during the effect that table 4 provides for the embodiment of the invention is tested.
Respectively organize the sleep incidence rate of mice in the effect experiment that table 4 embodiment of the invention provides
As shown in Table 4, ganoderma-lucidum medicinal mycoplasm can improve the sleep incidence rate of mice, and effect is better than the mixture of Radix Ginseng, Ganoderma, Radix Ginseng and Ganoderma.
3.6 shorten pentobarbital sodium sleep experiment incubation period
Choose 50 mices, be divided into 5 groups at random, be respectively Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and matched group;
By the dosage of 1.0g/kg body weight respectively with Radix Ginseng solution, Ganoderma solution, ginseng and lucid ganoderma mixed liquor, be subjected to reagent thing solution and distilled water to irritate stomach to give each mice in Radix Ginseng experimental group, Ganoderma experimental group, Radix Ginseng and Ganoderma combined experiments group, ganoderma-lucidum medicinal mycoplasm experimental group and the matched group, irritated stomach 30 days.The last filling behind the stomach behind the 20min, each organizes the pentobarbital sodium of mouse peritoneal injection 230mg/kg body weight, injection volume is 0.2mL/20g, with the righting reflex loss is index, observes and write down the sleep latent time of mice, adopts DAS2.0 statistics software that each data is carried out Treatment Analysis, adopt the t check, inspection level α=0.05 the results are shown in Table 5, respectively organizes the sleep latent time of mice during the effect that table 5 provides for the embodiment of the invention is tested.
Respectively organize the sleep latent time of mice in the effect experiment that table 5 embodiment of the invention provides
As shown in Table 5, ganoderma-lucidum medicinal mycoplasm can shorten the sleep latent time of mice, and effect is better than the mixture of Radix Ginseng, Ganoderma, Radix Ginseng and Ganoderma.
By The above results as can be known, ganoderma-lucidum medicinal mycoplasm can increase the length of one's sleep, raising sleep incidence rate, the shortening sleep latent time of mice, and effect all is better than the mixture of Radix Ginseng, Ganoderma, Radix Ginseng and Ganoderma.
Embodiment 4
Get the ganoderma-lucidum medicinal mycoplasm 50g of embodiment preparation, add 10g starch, mix homogeneously, tabletting obtains tablet.
Described tablet is configured to reagent solution, carry out toxicity test and effect experiment with reference to the experimental technique in embodiment 2 and the enforcement 3, prove the tablet avirulence that described Ganoderma medicinal fungal substance and starch obtain, and can increase the length of one's sleep, raising sleep incidence rate, the shortening sleep latent time of mice.
Comparative example 1
Radix Ginseng's pulverizing that will be from the Changchun grand inspection medical material company buys obtains the yellow-white powder;
Described powder is differentiated at microscopically visible powder has following feature: calcium oxalate cluster crystal diameter 20 μ m～68 μ m, corner angle are sharp-pointed; The resin canal fragment contains block yellow secretions in a tubular form; Cork cell's class is square, and wall is thin, wavy bending; Many reticulate patterns of conduit or scalariform.
According to the method that provides in the Chinese Pharmacopoeia described powder is carried out thin-layer chromatographic analysis, the chromatograph that obtains is compared with Radix Ginseng control medicinal material chromatograph, at ginsenoside Rb
1, ginsenoside Re, ginsenoside Rg
1The relevant position on show and to illustrate the point of same color and to contain ginsenoside Rb in the described powder
1, ginsenoside Re, ginsenoside Rg
1
Utilize the TU1810 ultraviolet-uisible spectrophotometer of Beijing Puxi General Instrument Co., Ltd that described powder is analyzed, the content that records total polysaccharides, total saponins and total protein in the powder is respectively 12wt%, 3.8wt% and 4.5wt%.
The ganoderma-lucidum medicinal mycoplasm of conventional ginseng and the embodiment of the invention 1 preparation is compared, and the result is referring to table 6, the comparison of the conventional ginseng that ganoderma-lucidum medicinal mycoplasm that table 6 provides for the embodiment of the invention and comparative example provide.
The comparison of the conventional ginseng that ganoderma-lucidum medicinal mycoplasm that table 6 embodiment of the invention provides and comparative example provide
As shown in Table 6, contain mycelium in the ganoderma-lucidum medicinal mycoplasm that the embodiment of the invention provides, and in the chemical constituent, the content of total saponins and conventional ginseng content do not have significant difference, but the content of total polysaccharides and total protein increases to some extent all.
The explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
Claims (8)
1. a ganoderma-lucidum medicinal mycoplasm is characterized in that, described ganoderma-lucidum medicinal mycoplasm is prepared by following method:
With adding water-wet after the Radix Ginseng pulverizing, obtain fermentation substrate after the sterilization;
With carrying out amplification culture after the Ganderma lucidum strain activation, obtain fermented bacterium;
Described fermented bacterium is inoculated in the fermentation substrate, under 24 ℃～28 ℃, the condition of lucifuge, carried out two-way solid fermentation 10 days～20 days.
2. a pharmaceutical preparation is characterized in that, comprises described ganoderma-lucidum medicinal mycoplasm of claim 1 and pharmaceutically useful carrier, and the mass ratio of described ganoderma-lucidum medicinal mycoplasm and described pharmaceutically useful carrier is 1: 0.1～3.
3. pharmaceutical preparation according to claim 2 is characterized in that, described pharmaceutically useful carrier is starch, dextrin, cyclamate, Icing Sugar or Polyethylene Glycol.
4. pharmaceutical preparation according to claim 2 is characterized in that, described preparation is powder, granule, tablet, capsule, pill, drop pill, soft capsule or oral liquid.
5. a pharmaceutical composition is characterized in that, comprises the described ganoderma-lucidum medicinal mycoplasm of claim 1, pharmacological active substance and pharmaceutically useful carrier.
6. pharmaceutical composition according to claim 5 is characterized in that, described pharmacological active substance is one or more in Lentinus Edodes, Semen Nelumbinis, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Polygalae and the above-mentioned substance extract.
7. a food compositions is characterized in that, comprises that described ganoderma-lucidum medicinal mycoplasm of claim 1 and threpsology go up acceptable food additive.
8. food compositions according to claim 7 is characterized in that, described food additive is one or more in starch, dextrin, cyclamate, Icing Sugar and the Polyethylene Glycol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101760530A CN101829172B (en) | 2010-05-19 | 2010-05-19 | Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101760530A CN101829172B (en) | 2010-05-19 | 2010-05-19 | Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101829172A true CN101829172A (en) | 2010-09-15 |
| CN101829172B CN101829172B (en) | 2012-07-25 |
Family
ID=42713493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101760530A Expired - Fee Related CN101829172B (en) | 2010-05-19 | 2010-05-19 | Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101829172B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102614238A (en) * | 2012-04-18 | 2012-08-01 | 邱智东 | Application of lucide ganoderma and ginseng medicinal mycoplasm in preparing drugs for treating lung cancer |
| CN103202460A (en) * | 2012-01-16 | 2013-07-17 | 张平 | Method for biological fermentation of silybum marianum by using edible fungi |
| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103908489A (en) * | 2014-03-11 | 2014-07-09 | 中国科学院长春应用化学研究所 | Bidirectional solid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm |
| CN105925655A (en) * | 2016-06-27 | 2016-09-07 | 中国农业科学院特产研究所 | Method for obtaining rare ginsenoside by fermentation of Ganoderma tsugae liquid |
| CN109456383A (en) * | 2018-12-04 | 2019-03-12 | 长春中医药大学 | A kind of 20(R)-ginsenoside Rh1Preparation method |
| CN112641093A (en) * | 2020-12-31 | 2021-04-13 | 厦门市天德善品生物科技有限公司 | Cordyceps sinensis ginseng mushroom fungus lignin, health food and preparation method thereof |
| CN112772914A (en) * | 2020-12-31 | 2021-05-11 | 厦门市天德善品生物科技有限公司 | Cordyceps sinensis ginseng mushroom grass nutrient and preparation method thereof |
| CN113287707A (en) * | 2021-05-28 | 2021-08-24 | 长春中医药大学 | Ginseng and hericium erinaceus bidirectional solid fermentation mycoplasm solid beverage and preparation method thereof |
| CN113288831A (en) * | 2021-06-11 | 2021-08-24 | 北京工商大学 | Application of ganoderma lucidum and/or ginseng in preparation of anti-inflammatory factor product and skin care product or cosmetic thereof |
| CN113349368A (en) * | 2021-05-28 | 2021-09-07 | 长春中医药大学 | Preparation process and application of hericium erinaceus-ginseng fermentation mycoplasm enzyme oral liquid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101449803A (en) * | 2008-12-26 | 2009-06-10 | 山西瑞芝生物科技有限公司 | Ganoderma lucidum powder production method |
-
2010
- 2010-05-19 CN CN2010101760530A patent/CN101829172B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101449803A (en) * | 2008-12-26 | 2009-06-10 | 山西瑞芝生物科技有限公司 | Ganoderma lucidum powder production method |
Non-Patent Citations (1)
| Title |
|---|
| 《中国食用菌》 20021231 庄毅 药用真菌新型（双向型）固体发酵工程 第4页 1-8 第21卷, 第4期 2 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202460A (en) * | 2012-01-16 | 2013-07-17 | 张平 | Method for biological fermentation of silybum marianum by using edible fungi |
| CN102614238A (en) * | 2012-04-18 | 2012-08-01 | 邱智东 | Application of lucide ganoderma and ginseng medicinal mycoplasm in preparing drugs for treating lung cancer |
| WO2013155885A1 (en) * | 2012-04-18 | 2013-10-24 | Qiu Zhidong | Application of lucide ganoderma and ginseng medicinal mycoplasm in preparing drugs for treating lung cancer |
| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103908489A (en) * | 2014-03-11 | 2014-07-09 | 中国科学院长春应用化学研究所 | Bidirectional solid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm |
| CN105925655A (en) * | 2016-06-27 | 2016-09-07 | 中国农业科学院特产研究所 | Method for obtaining rare ginsenoside by fermentation of Ganoderma tsugae liquid |
| CN109456383A (en) * | 2018-12-04 | 2019-03-12 | 长春中医药大学 | A kind of 20(R)-ginsenoside Rh1Preparation method |
| CN109456383B (en) * | 2018-12-04 | 2021-06-22 | 长春中医药大学 | 20(R) -ginsenoside Rh1Preparation method of (1) |
| CN112641093A (en) * | 2020-12-31 | 2021-04-13 | 厦门市天德善品生物科技有限公司 | Cordyceps sinensis ginseng mushroom fungus lignin, health food and preparation method thereof |
| CN112772914A (en) * | 2020-12-31 | 2021-05-11 | 厦门市天德善品生物科技有限公司 | Cordyceps sinensis ginseng mushroom grass nutrient and preparation method thereof |
| CN113287707A (en) * | 2021-05-28 | 2021-08-24 | 长春中医药大学 | Ginseng and hericium erinaceus bidirectional solid fermentation mycoplasm solid beverage and preparation method thereof |
| CN113349368A (en) * | 2021-05-28 | 2021-09-07 | 长春中医药大学 | Preparation process and application of hericium erinaceus-ginseng fermentation mycoplasm enzyme oral liquid |
| CN113288831A (en) * | 2021-06-11 | 2021-08-24 | 北京工商大学 | Application of ganoderma lucidum and/or ginseng in preparation of anti-inflammatory factor product and skin care product or cosmetic thereof |
| CN113288831B (en) * | 2021-06-11 | 2023-08-29 | 北京工商大学 | Application of ganoderma lucidum and/or ginseng in preparing anti-inflammatory factor product and skin care product or cosmetic thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101829172B (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101829172B (en) | Ganoderma-lucidum medicinal mycoplasm, pharmaceutical preparation, pharmaceutical composition and food composition | |
| CN103315362B (en) | Poria cocos solid state fermentation functional beverage and its preparation method | |
| CN101254163A (en) | Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product | |
| CN103141702B (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN101284122A (en) | Soft capsules for resisting the climate sickness | |
| CN106722105A (en) | A kind of selenium-rich glasswort health nutritious noodle and its production technology | |
| CN105410346A (en) | Cultured penaeus vannamei survival rate improving traditional Chinese medicine preparation and preparation method thereof | |
| CN101816689B (en) | Processing method of ginseng | |
| CN102613331B (en) | Halimasch fermented tea and halimasch fermented tea composition, and preparation methods thereof | |
| CN109652271A (en) | A kind of production method of health care Spondias axillaris fruit wine | |
| CN103948023B (en) | The health food of a kind of develop immunitypty and improving water flood and two-step fermentation preparation method thereof | |
| CN103315359B (en) | Grifola frondosus solid state fermentation functional beverage and its preparation method | |
| CN103932181B (en) | A kind ofly to release the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof | |
| CN104000192B (en) | A kind of functional food and two-step fermentation preparation method thereof releasing the pressure and regulate stomach | |
| CN103169127A (en) | Beverage prepared from honeysuckle flowers, chrysanthemums and peonies | |
| CN104799286A (en) | Composition for improving immunity and preparation method of composition | |
| CN102177996B (en) | Semen cannabis laxative tea and preparation method thereof | |
| CN103932180B (en) | A kind ofly to release the pressure and the health food of antifatigue and two-step fermentation preparation method thereof | |
| CN103932179B (en) | A kind of health food and two-step fermentation preparation method thereof releasing the pressure and protect liver | |
| CN106924548A (en) | The medicine and preparation method of a kind of fatigue-relieving | |
| CN103330203A (en) | Functional black fungus and kudzuvine root food and preparation method thereof | |
| CN104381992A (en) | Eucommia ulmoides olive fungal substance sugar-free food and preparation method thereof | |
| CN103695322A (en) | Phellinus igniarius mycelia product containing rich bioactive substances and production method thereof | |
| CN107223966A (en) | It is a kind of to be used to improve sweet oral liquid of Rong's grass of immunity and preparation method thereof | |
| CN105748610A (en) | Solid fermentation method for polygonum hydropiper linn preparations by aid of bacillus subtilis, fermentation products of solid fermentation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120725 |