CN101828481B - Method for preparing edible mushroom culture bar - Google Patents
Method for preparing edible mushroom culture bar Download PDFInfo
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- CN101828481B CN101828481B CN2010101596930A CN201010159693A CN101828481B CN 101828481 B CN101828481 B CN 101828481B CN 2010101596930 A CN2010101596930 A CN 2010101596930A CN 201010159693 A CN201010159693 A CN 201010159693A CN 101828481 B CN101828481 B CN 101828481B
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- edible mushroom
- mushroom culture
- sterilization
- heat exchange
- culture bar
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Abstract
The invention discloses a method for preparing an edible mushroom culture bar, which comprises the following steps: (1) crushing and uniformly stirring substrate raw materials; (2) heating, pressurizing and sterilizing the raw materials and reducing the pressure of the raw materials to a normal pressure with stirring; (3) performing surface heat exchange and reducing a temperature; (4) adding sterilized substrate components which cannot withstand high temperature and high pressure, adjusting water content and uniformly stirring the mixture; (5) inoculating liquid strains with stirring; and (6) bagging the strains to obtain the edible mushroom culture bar. As the surface heat exchange method is adopted to reduce the temperature, the method, compared with the prior art, has the advantages that: cooling water does not need sterilizing and can be recycled; heat generated by the surface heat exchange can be recycled, which saves the heat and shortens cooling time; labor required for bag-by-bag injection addition with an injector is saved; and sterilizing time is short, and labor intensity and cost are low.
Description
Technical field
The invention belongs to the Edible Fungi technology, particularly relate to a kind of preparation method of edible mushroom culture bar.
Background technology
In the at present domestic and international Edible Fungi, bottle is planted the medium production of kind and is generally adopted following process route:
Various culture medium raw materials are pulverized respectively------pack---heating pressurization sterilization---cooling---inoculation---finished product with the mixing in proportion of the culture medium raw material after the various pulverizing.There are the following problems for this method:
1. the pressurization sterilization of heating is carried out after the culture medium raw material pack, and some non-refractory or not high voltage bearing culture medium raw material can not add before sterilization, can only use injector to inject to add by bag after cooling.
2. the culture medium raw material after the pack is sterilized in the high-temperature sterilization still, because of intensive the piling up in the high-temperature sterilization still of sack that culture medium raw material is housed, cold air between cold air in the bag or bag is difficult for exhaust, can make packed culture medium raw material contact high steam inhomogeneous, the sterilization effect of retardance steam.Needing increases sterilization time, but the prolongation of sterilization time can make the nutrient component of culture medium raw material decompose.
3. be loaded in the bag owing to culture medium raw material, can not open the high-temperature sterilization still immediately behind the high-temperature sterilization, otherwise easily explosion of bag, and need in still, be cooled to set point of temperature with the sterilization cold water sprays, because the culture medium raw material bag is little with sterilization cold water contact area, so cooling rate is slow, in this process, heat energy can not be collected utilization, the cold water consumption of sterilizing is many, system sterilization cold water also needs big energy, and simultaneously, temperature fall time is longer.
4. owing to autoclave sterilization, the material of therefore adorning the sack of culture medium raw material needs high-temperature-resistant high-pressure-resistant (as polypropylene), and this material is than the not high voltage bearing material of non-refractory (as polyethylene) price height, and easily fragmentation.
5. be loaded on can not long-term storage in the bag for culture medium raw material, is sterilization treatment after packing, particularly in the season of weather sweltering heat, this be because, culture medium raw material is loaded in the bag, bacterium produces acid at suitable temperature and humidity condition bottom fermentation.
6. inoculation is to adopt injection inoculation or transfer needle inoculation, and postvaccinal bacterial classification is linear distribution in the culture medium raw material bag.
7. inoculation as employing manual operations, labour intensity is big, the cost height; If adopt existing automatic vaccination machine operation, complicated operation, equipment cost height.
Above-mentioned deficiency, the production technology of present edible mushroom culture bar are unsuitable in enormous quantities, suitability for industrialized production owing to existing.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of preparation method of edible mushroom culture bar is provided.
Technical scheme of the present invention is summarized as follows:
The preparation method of edible mushroom culture bar, be made up of following step:
(1) culture medium raw material is pulverized mixing respectively;
(2) under agitation, the pressurization sterilization of heating is decompressed to normal pressure;
(3) partition heat exchange cooling;
(4) the not high voltage bearing medium component of non-refractory of adding degerming is adjusted moisture content, stirs;
(5) under agitation, inoculation liquid spawn;
(6) a kind of edible mushroom culture bar is promptly made in pack.
Advantage of the present invention:
1. because culture medium raw material of the present invention is in the heat pressurization sterilization of row of advancing of pack not, therefore do not exist the culture medium raw material bag situation of explosion to occur when cooling, can adopt the partition heat-exchange method to lower the temperature, compared with prior art: cooling water need not sterilization; Cooling water can cycling and reutilization; The heat of partition heat exchange can utilize again; Save a large amount of cooling waters, saved heat, shortened cool time.
2. the not high voltage bearing medium component of non-refractory is by behind the membrane filtration, and disposable interpolation has been saved by bag and used injector to inject to add required labour.
3. the disposable inoculation of liquid spawn is uniformly distributed in the culture medium raw material bacterial classification, has overcome the linear distribution situation of prior art.
4. culture medium raw material is sterilized before pack, has avoided the uneven phenomenon of culture medium raw material bag contact high steam, and disinfecting time is few, has saved quantity of steam.
5. the present invention can adopt the not bag of high voltage bearing material of price is low, suppleness is good non-refractory.
6. the present invention adopts mechanically actuated, has replaced manual operations of the prior art, and labour intensity is little, and cost is low.
Description of drawings
Fig. 1 is a process chart of the present invention.
Embodiment
The present invention is further illustrated below in conjunction with drawings and Examples.Following given example just is used for illustrating the present invention, but and limit the present invention, by method of the present invention, adopt corresponding apparatus to prepare edible mushroom culture bar and all belong to protection scope of the present invention.
Embodiment 1
The preparation method of edible mushroom culture bar, be made up of following step:
(1) get by weight ratio: cotton seed hulls 70%, corncob 6%, calcium carbonate 2% and wheat bran 22% are pulverized mixing;
(2) pack in the material transport vehicle 8, enter the Sterilization Kettle 1 from Sterilization Kettle feeding gate 2;
(3) airtight Sterilization Kettle under agitation, feeds steam and is warming up to 120-160 ℃, sterilization 30min is decompressed to normal pressure, and material transport vehicle is shifted out from the Sterilization Kettle discharge door 3 that is arranged on clean area, upset, discharging to the bottom that has chuck is provided with in the agitator tank 4 of discharge gate;
(4) in the bottom that has chuck is provided with the agitator tank of discharge gate, be cooled to room temperature, open the discharge gate discharging to inoculating in the agitator tank 5 with the heat exchange of cooling water partition;
(5) add the vitamin B1 aqueous solution of an amount of degerming, adjust moisture content, make moisture reach 65% of quality of material, stir;
(6) under agitation, inoculation liquid spawn Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) is sent in the sack filling machine 7 by stirring cage 6;
(7) a kind of edible mushroom culture bar is promptly made in pack.(the Gleoesterum incarnatum bacterial classification is purchased in Chinese agriculture microorganism center, bacterium ACCC503940 among the present invention)
Embodiment 2
The preparation method of edible mushroom culture bar, be made up of following step:
(1) get by weight ratio:: corncob 70%, cotton seed hulls 10%, wheat bran 15%, corn flour 4%, gypsum 1% is pulverized mixing;
(2) pack in the material transport vehicle, enter the Sterilization Kettle from the Sterilization Kettle feeding gate;
(3) airtight Sterilization Kettle under agitation, is warming up to 120-160 ℃, and sterilization 40min is decompressed to normal pressure, material transport vehicle is shifted out from the Sterilization Kettle discharge door that is arranged on clean area, and upset, discharging is provided with in the agitator tank of discharge gate to the bottom that has chuck;
(4) in the bottom that has chuck is provided with the agitator tank of discharge gate, be cooled to room temperature, open the discharge gate discharging to inoculating in the agitator tank with the heat exchange of cooling water partition;
(5) corn flour is added an amount of water, heat and boil into scattered paste shape, add in the agitator tank, add the vitamin B1 aqueous solution of an amount of degerming again, adjust moisture content, make moisture reach 65% of material mass, stir;
(6) under agitation, inoculation liquid spawn Ji mushroom is sent in the sack filling machine 7 by stirring cage 6;
(7) a kind of edible mushroom culture bar is promptly made in pack.
Embodiment 3
The preparation method of edible mushroom culture bar, be made up of following step:
(1) get by weight ratio: cotton seed hulls 55%, wood chip 20%, wheat bran 18.5%, corn flour 5%, sucrose 0.5%, calcium carbonate 1% is pulverized mixing;
(2) pack in the material transport vehicle, enter the Sterilization Kettle from the Sterilization Kettle feeding gate;
(3) airtight Sterilization Kettle under agitation, feeds steam and is warming up to 120-160 ℃, sterilization 20min is decompressed to normal pressure, and material transport vehicle is shifted out from the Sterilization Kettle discharge door that is arranged on clean area, upset, discharging is provided with in the agitator tank of discharge gate to the bottom that has chuck;
(4) in the bottom that has chuck is provided with the agitator tank of discharge gate, be cooled to room temperature, open the discharge gate discharging to inoculating in the agitator tank with the heat exchange of cooling water partition;
(5) corn flour, sucrose are added an amount of water, heat and boil into scattered paste shape, add in the agitator tank, add the vitamin B1 aqueous solution of an amount of degerming, adjust moisture content, make moisture contain quality and reach 65%, stir;
(6) under agitation, inoculation liquid spawn Xingbao mushroom bacterium is sent in the sack filling machine 7 by stirring cage 6;
(7) a kind of edible mushroom culture bar is promptly made in pack.
Experimental results show that: the edible mushroom culture bar with the foregoing description preparation improves more than 10% the edible mushroom productive rate, and production efficiency is enhanced about more than once.
The operation of each equipment from the Sterilization Kettle discharge door to sack filling machine is all carried out in clean area.
Edible mushroom culture bar culture medium raw material composition of the present invention, can be according to the difference of the kind of the edible mushroom that will cultivate and difference (as many medium are arranged in the prior art), people only need the not high voltage bearing medium component of the non-refractory in the raw material composition is picked out, the sterilization of heating earlier of other composition, add the not high voltage bearing medium component of non-refractory of degerming again, inoculate liquid spawn, again pack, make whole preparation method simple, easy to operate.
Claims (1)
1. the preparation method of edible mushroom culture bar is characterized in that being made up of following step:
(1) culture medium raw material is pulverized mixing respectively;
(2) under agitation, the pressurization sterilization of heating is decompressed to normal pressure;
(3) partition heat exchange cooling;
(4) the not high voltage bearing medium component of non-refractory of adding degerming is adjusted moisture content, stirs;
(5) under agitation, inoculation liquid spawn;
(6) a kind of edible mushroom culture bar is promptly made in pack.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101596930A CN101828481B (en) | 2010-04-29 | 2010-04-29 | Method for preparing edible mushroom culture bar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101596930A CN101828481B (en) | 2010-04-29 | 2010-04-29 | Method for preparing edible mushroom culture bar |
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| Publication Number | Publication Date |
|---|---|
| CN101828481A CN101828481A (en) | 2010-09-15 |
| CN101828481B true CN101828481B (en) | 2011-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010101596930A Expired - Fee Related CN101828481B (en) | 2010-04-29 | 2010-04-29 | Method for preparing edible mushroom culture bar |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640660A (en) * | 2012-05-02 | 2012-08-22 | 张春平 | Machine for conveying, blending, stirring, rod making, bagging of edible fungi raw materials and auxiliary materials |
| CN102838400A (en) * | 2012-09-11 | 2012-12-26 | 山西奥格姆农业科技有限公司 | Method for secondary utilization of edible mushrooms culture medium |
| CN104956918B (en) * | 2015-07-08 | 2018-04-17 | 翔天农业开发集团股份有限公司 | A kind of preparation method of lentinus edodes strain stick |
| CN107258316A (en) * | 2016-04-08 | 2017-10-20 | 单文修 | A kind of bacterium material process units |
| CN108849254A (en) * | 2018-09-16 | 2018-11-23 | 杭州裕登农业技术开发有限公司 | A kind of double helix bacterium bag loader |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044572A (en) * | 1989-09-23 | 1990-08-15 | 山东垦利县科学技术委员会 | Seed preparation method of edible fungi semi-mature material |
| CN101455161A (en) * | 2007-12-13 | 2009-06-17 | 辽宁东方农业科技有限公司 | Method for producing north half-clinker open type mushroom |
-
2010
- 2010-04-29 CN CN2010101596930A patent/CN101828481B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044572A (en) * | 1989-09-23 | 1990-08-15 | 山东垦利县科学技术委员会 | Seed preparation method of edible fungi semi-mature material |
| CN101455161A (en) * | 2007-12-13 | 2009-06-17 | 辽宁东方农业科技有限公司 | Method for producing north half-clinker open type mushroom |
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| CN101828481A (en) | 2010-09-15 |
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