CN101821402B - 修剪式多位点组合装配 - Google Patents

修剪式多位点组合装配 Download PDF

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Publication number
CN101821402B
CN101821402B CN200880101253.0A CN200880101253A CN101821402B CN 101821402 B CN101821402 B CN 101821402B CN 200880101253 A CN200880101253 A CN 200880101253A CN 101821402 B CN101821402 B CN 101821402B
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CN
China
Prior art keywords
primer
polynucleotide
primers
group
sudden change
Prior art date
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Expired - Fee Related
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CN200880101253.0A
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English (en)
Chinese (zh)
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CN101821402A (zh
Inventor
X·谭
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BASF Enzymes LLC
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Bp Corp North America Co ltd
Verenium Corp
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Application filed by Bp Corp North America Co ltd, Verenium Corp filed Critical Bp Corp North America Co ltd
Publication of CN101821402A publication Critical patent/CN101821402A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN200880101253.0A 2007-07-31 2008-07-31 修剪式多位点组合装配 Expired - Fee Related CN101821402B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95317107P 2007-07-31 2007-07-31
US60/953,171 2007-07-31
PCT/US2008/071771 WO2009018449A1 (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201510050333.XA Division CN104673784A (zh) 2007-07-31 2008-07-31 修剪式多位点组合装配

Publications (2)

Publication Number Publication Date
CN101821402A CN101821402A (zh) 2010-09-01
CN101821402B true CN101821402B (zh) 2015-03-04

Family

ID=40304880

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201510050333.XA Pending CN104673784A (zh) 2007-07-31 2008-07-31 修剪式多位点组合装配
CN200880101253.0A Expired - Fee Related CN101821402B (zh) 2007-07-31 2008-07-31 修剪式多位点组合装配

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201510050333.XA Pending CN104673784A (zh) 2007-07-31 2008-07-31 修剪式多位点组合装配

Country Status (16)

Country Link
US (1) US9476078B2 (enExample)
EP (2) EP3031919B1 (enExample)
JP (1) JP6014805B2 (enExample)
KR (1) KR101685712B1 (enExample)
CN (2) CN104673784A (enExample)
AU (1) AU2008282101A1 (enExample)
BR (1) BRPI0815046A2 (enExample)
CA (1) CA2693102C (enExample)
DK (1) DK3031919T3 (enExample)
EA (1) EA020657B1 (enExample)
ES (1) ES2701883T3 (enExample)
IL (1) IL202839A0 (enExample)
MX (1) MX353478B (enExample)
NZ (1) NZ582876A (enExample)
WO (1) WO2009018449A1 (enExample)
ZA (1) ZA201000152B (enExample)

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US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010135588A2 (en) 2009-05-21 2010-11-25 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
BR112013008347A2 (pt) 2010-10-06 2016-06-14 Bp Corp North America Inc polipeptídeos de variantes cbh i
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
JP2016525354A (ja) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー フィターゼ
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
CN111278970B (zh) 2017-10-25 2024-09-27 巴斯夫欧洲公司 β-淀粉酶
US12110489B2 (en) 2017-12-20 2024-10-08 Basf Se Gene site saturation mutagenesis (GSSM) method
US11634698B2 (en) 2017-12-20 2023-04-25 Basf Se Nucleases and methods for making and using them
CN112368375A (zh) 2018-04-26 2021-02-12 巴斯夫欧洲公司 脂肪酶
WO2019229101A1 (en) 2018-05-29 2019-12-05 Basf Se Amylase enzymes
EP3847264A1 (en) 2018-09-07 2021-07-14 Basf Plant Science Company GmbH Improved method for the production of high levels of pufa in plants
US12460221B2 (en) 2018-09-07 2025-11-04 Basf Plant Science Company Gmbh Method for the production of high levels of PUFA in plants
CN112996915A (zh) 2018-09-07 2021-06-18 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
EP3938504A1 (en) 2019-03-11 2022-01-19 Basf Se Amylases and methods for making and using them
US20220220463A1 (en) 2019-04-23 2022-07-14 Basf Se Beta-amylase variants
MX2022007714A (es) 2019-12-20 2022-07-19 Basf Se Disminucion de la toxicidad de terpenos y aumento de la posible produccion en microorganismos.
MX2022015338A (es) 2020-06-04 2023-01-16 Isobionics B V Santaleno sintasas sinteticas.
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
MX2024001532A (es) 2021-08-02 2024-02-13 Basf Se Produccion novedosa de compuestos aromaticos con ionilidenoetano sintasas.

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US6773900B2 (en) * 1995-12-07 2004-08-10 Diversa Corporation End selection in directed evolution
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
US7202086B2 (en) * 2000-08-25 2007-04-10 Biomethodes Method for massive directed mutagenesis

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Also Published As

Publication number Publication date
EA201070206A1 (ru) 2010-06-30
NZ582876A (en) 2012-07-27
WO2009018449A1 (en) 2009-02-05
JP6014805B2 (ja) 2016-10-26
US9476078B2 (en) 2016-10-25
EP2183378A4 (en) 2010-09-22
EP2183378A1 (en) 2010-05-12
ZA201000152B (en) 2011-06-29
CN101821402A (zh) 2010-09-01
CA2693102A1 (en) 2009-02-05
CN104673784A (zh) 2015-06-03
DK3031919T3 (en) 2019-01-07
KR101685712B1 (ko) 2016-12-12
ES2701883T3 (es) 2019-02-26
US20100216192A1 (en) 2010-08-26
EP3031919B1 (en) 2018-09-19
KR20100045494A (ko) 2010-05-03
MX2010001182A (es) 2010-04-21
EP3031919A1 (en) 2016-06-15
JP2010535034A (ja) 2010-11-18
CA2693102C (en) 2020-03-10
IL202839A0 (en) 2010-06-30
EA020657B1 (ru) 2014-12-30
MX353478B (es) 2018-01-15
BRPI0815046A2 (pt) 2014-10-14
AU2008282101A1 (en) 2009-02-05

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C06 Publication
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ASS Succession or assignment of patent right

Owner name: BP CORPORATION NORTH AMERICA INC.

Free format text: FORMER OWNER: DIVERSA CORP.

Effective date: 20110915

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Effective date of registration: 20110915

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Applicant after: BP Corporation North America Co.,Ltd.

Address before: California, USA

Applicant before: VERENIUM Corp.

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C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160829

Address after: California, USA

Patentee after: BASF Enzymes LLC

Address before: indiana

Patentee before: BP Corporation North America Co.,Ltd.

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150304