CN101805695A - Oblique rotational gravity mixed biological reaction equipment - Google Patents
Oblique rotational gravity mixed biological reaction equipment Download PDFInfo
- Publication number
- CN101805695A CN101805695A CN 201010102619 CN201010102619A CN101805695A CN 101805695 A CN101805695 A CN 101805695A CN 201010102619 CN201010102619 CN 201010102619 CN 201010102619 A CN201010102619 A CN 201010102619A CN 101805695 A CN101805695 A CN 101805695A
- Authority
- CN
- China
- Prior art keywords
- culture vessel
- biological reaction
- stationary installation
- reaction apparatus
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
- C12M27/12—Roller bottles; Roller tubes
Abstract
The invention relates to oblique rotational gravity mixed biological reaction equipment, in particular to biological reaction equipment capable of enabling a container with a columnar main body to perform micro-carrier cell culture. In a frame which plays a role in fixing and supporting, a plurality of columnar culture vessel fixing devices which are parallel to one another and form angles of between 5 and 8 degrees with a horizontal plane are driven by an electric motor to rotate; a plurality of groups of fixing hole sites are symmetrically distributed around rotating shafts on the columnar culture vessel fixing devices and relatively to the centers of the rotating shafts; each group of hole sites consists of at least two fixing holes with centers on the same straight line and parallel planes and a supporting pedestal which has a supporting effect at the bottom; the lines or rows of columnar culture vessel fixing devices, the lines or rows of columnar culture vessel fixing devices and the electric motor can be driven by a transmission belt or a gear; the reaction equipment can comprise a control circuit part; and the reaction equipment can be accommodated in a small-sized temperature control vessel or arranged in a temperature control chamber.
Description
Technical field
The microcarrier cell cultures is the main method that mass cell is cultivated, and the conventional bio-reactor that is used for the microcarrier cell cultures costs an arm and a leg, and complex structure needs the operator of specialty to use.
The biological reaction apparatus that the present invention relates to is economical and practical, and is simple to operate, makes the microcarrier cell cultures easy as common cell bottle cell cultures.
Background technology
Because traditional rolling bottle culturing cell exists labor capacity big, required bottle quantity is many, and contaminating microorganisms consumes energy greatly easily, produces shortcomings such as dirt is many, and the microcarrier cell culture technology receives publicity day by day.And the bio-reactor of import costs an arm and a leg, and the home-made bio-reactor is cheap, the bad again assurance of culture effect and stability.
Summary of the invention
In order to address the above problem, the invention provides a kind of microcarrier biological reaction apparatus can be that column culture vessel (as 3L rolling bottle etc.) carries out the microcarrier cell cultures by main body, reaches high-cell density, economical and practical.
The technical solution adopted in the present invention is: a kind of main body that makes is the biological reaction apparatus that the container of column carries out the microcarrier cell cultures, in the framework that plays fixing and supporting role, rotate by motor drive 1 row or multirow, 1 row or multiple row column culture vessel stationary installation, carry out the microcarrier cell cultures.
Rotate about it axle on its column culture vessel stationary installation and relatively rotate a revenue centre and be symmetrically distributed with 2 groups, 3 groups or organize fixed hole position more, position, every group of hole by at least 2 centers on same straight line and plane, place fixed orifices parallel to each other and the base for supporting that plays the support effect in the bottom form.
Its each column culture vessel stationary installation parallel to each other and and horizontal plane be 5～80 the degree angles.
Between its each row or each colonnade shape culture vessel stationary installation, can use transmission belt transmission or geartransmission between each row or each colonnade shape culture vessel stationary installation and the electric motor.
It can contain the pilot circuit part.
It can be contained in the small-sized temperature control container, also it directly can be put in the temperature-controlled chamber.
Description of drawings
Fig. 1, Fig. 2 are the side-view of two kinds of types of realization the design thinking
Fig. 3 is the frontview of the column culture vessel stationary installation of horizontal positioned
Fig. 4 is the schematic perspective view of spending the column culture vessel stationary installation at angles with horizontal plane journey 5～80
Fig. 5 is the framework of its fixed support effect of frontview (1) of multiple lines and multiple rows and column culture vessel stationary installations horizontal plane journey 5～80 degree angles; (2) electric motor; (3) column culture vessel stationary installation; (4) crossbeam; (5) belt or the gear of connection column culture vessel stationary installation; (6) belt or the gear of connection column culture vessel stationary installation and electric motor; (7) fixed hole position of placement column culture vessel; (8) column culture vessel stationary installation rotation axis; (9) fixed orifices of placement column culture vessel; (10) base for supporting of placement column culture vessel;
Embodiment
For the features and advantages of the present invention can be become apparent more, the present invention is further detailed explanation below in conjunction with the drawings and specific embodiments.
Fig. 1, Fig. 2 are the side-view of two kinds of types of realization the design thinking, connecting on the crossbeam (4) in framework (1) by 1 row or multirow, 1 row or multiple row is parallel to each other and with the column culture vessel stationary installation (3) at horizontal plane journey 5～80 degree angles, every row or every capable column culture vessel stationary installation (3) are connected by belt or gear (5), and be connected to common electric motor (2) by belt or gear (6), rotate by pilot circuit control motor drive.
Fig. 3 is the frontview of the column culture vessel stationary installation of horizontal positioned, the fixed hole position (7) that 2 groups, 3 groups or many groups are placed the column culture vessels round its revenue centre symmetric offset spread of rotation axis (8).Fig. 4 is the schematic perspective view of spending the column culture vessel stationary installation at angles with horizontal plane journey 5～80, at least 2 fixed orifices (9) and 1 base for supporting (10) of placing the column culture vessel of placing the column culture vessels on the fixed hole position of each placement column culture vessel.
Fig. 5 is frontviews multiple lines and multiple rows and column culture vessel stationary installations horizontal plane journey 5～80 degree angles.
Claims (7)
- One kind can to make main body be the biological reaction apparatus that the culture vessel of column carries out the microcarrier cell cultures, in the framework that plays fixing and supporting role, by the motor drive multirow, multiple row column culture vessel stationary installation is rotated, carry out the microcarrier cell cultures, each column culture vessel stationary installation is parallel to each other and all tilt to be connected in the support frame.
- 2. biological reaction apparatus according to claim 1, on the column culture vessel stationary installation of its inclination, rotate about it axle and relatively rotate a revenue centre and be symmetrically distributed with many group fixed hole positions, every group of fixed hole position by at least two centers on same straight line and plane, place fixed orifices parallel to each other and the base for supporting that plays the support effect in the bottom form.
- 3. according to any described biological reaction apparatus in the claim 1～2, its each column culture vessel stationary installation be parallel to each other and and horizontal plane be 5～80 degree angles.
- 4. according to any described biological reaction apparatus in the claim 1～2, between the column culture vessel stationary installation that Qi Gehang, each row tilt, can use the transmission belt transmission between column culture vessel stationary installation that each row, each row tilt and the electric motor.
- 5. according to any described biological reaction apparatus in the claim 1～2, it contains the pilot circuit part, and pilot circuit comprises power switch, electromotor velocity regulating circuit, corresponding button.
- 6. according to any described biological reaction apparatus in the claim 1～2, it can be placed in the temperature control container.
- 7. the temperature regulator according to claim 6 a described placement biological reaction apparatus comprises greenhouse, incubator etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010102619 CN101805695B (en) | 2010-01-29 | 2010-01-29 | Oblique rotational gravity mixed biological reaction equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010102619 CN101805695B (en) | 2010-01-29 | 2010-01-29 | Oblique rotational gravity mixed biological reaction equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101805695A true CN101805695A (en) | 2010-08-18 |
| CN101805695B CN101805695B (en) | 2012-06-13 |
Family
ID=42607650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010102619 Active CN101805695B (en) | 2010-01-29 | 2010-01-29 | Oblique rotational gravity mixed biological reaction equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101805695B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521946A (en) * | 2015-01-26 | 2015-04-22 | 深圳康泰生物制品股份有限公司 | Cell freezing device and method |
| CN109196086A (en) * | 2016-03-14 | 2019-01-11 | R·D·帕特尔 | bioreactor system and its method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1286966C (en) * | 2003-12-10 | 2006-11-29 | 上海伯瑞生物技术发展有限公司 | Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture |
| CN1321175C (en) * | 2004-01-13 | 2007-06-13 | 中国科学院动物研究所 | Microcarrier for large-scale culture cell |
| CN100360657C (en) * | 2005-03-30 | 2008-01-09 | 韩毅鹏 | Horizontal drum type bioreactor |
| CN200949106Y (en) * | 2006-09-22 | 2007-09-19 | 韩毅鹏 | Tilted two-level rotating cylinder bioreactor |
| CN201753358U (en) * | 2010-01-29 | 2011-03-02 | 福尔生物制药股份有限公司 | Oblique and rotary gravity mixed biological reaction unit |
-
2010
- 2010-01-29 CN CN 201010102619 patent/CN101805695B/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521946A (en) * | 2015-01-26 | 2015-04-22 | 深圳康泰生物制品股份有限公司 | Cell freezing device and method |
| CN109196086A (en) * | 2016-03-14 | 2019-01-11 | R·D·帕特尔 | bioreactor system and its method |
| CN109196086B (en) * | 2016-03-14 | 2022-04-26 | Omnibrx生物科技私人有限公司 | Bioreactor system and method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101805695B (en) | 2012-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100304474A1 (en) | Rotation Driving Device for Cell Culturing | |
| CN101805695B (en) | Oblique rotational gravity mixed biological reaction equipment | |
| CN201990682U (en) | Swinging type cell culture device | |
| CN202201907U (en) | Cell stress culture apparatus | |
| CN113366099A (en) | Carousel for modular bio-production units | |
| CN101805694B (en) | Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel | |
| CN201753358U (en) | Oblique and rotary gravity mixed biological reaction unit | |
| CN103305424B (en) | Variable-amplitude rotary cell culture table | |
| CN103555561B (en) | Biomechanical generator | |
| CN201634681U (en) | Gravity hybrid biological reaction device slantwise rotating along central shaft of culture container | |
| CN206253086U (en) | Multiple field cyclotron oscillation device receptacle stand | |
| CN202465713U (en) | Low-speed stirring system for cell culture | |
| CN204220178U (en) | A kind of rotatable alternating temperature rack for test tube | |
| CN203184053U (en) | Rotating disc test tube rack | |
| CN217025936U (en) | Cell culture bottle | |
| CN207330957U (en) | A kind of vortex oscillation blending device | |
| CN101469319B (en) | Method for producing effective bioreactor | |
| CN103289898B (en) | Elliptical orbit type cell culture oscillator | |
| CN204608022U (en) | There is the culture dish strengthening observing capacity ware lid | |
| CN211005372U (en) | Research contrast device of selenium-enriched oyster mushroom polysaccharide to probiotic growth metabolism | |
| CN105112294A (en) | Coaxial multi-incubator bioreactor | |
| CN202315947U (en) | Integrated reagent bottle and reagent tube multipurpose stand | |
| CN203559059U (en) | Full-automatic three-dimensional cell culturing system | |
| CN202089965U (en) | Novel solid-state fermentation tank | |
| CN219032127U (en) | Novel culture dish observation table |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: ZHONGKE BIOLOGICAL PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: FORWELL BIOPHARMACEUTICALS CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 065201 No. 1155 Development Zone, Sanhe, Hebei, Yanjiao Patentee after: ZHONGKE BIOPHARM CO.,LTD. Address before: 065201 Yingbin Road 38-2, Yanjiao Development Zone, Hebei, Sanhe Patentee before: FORWELL BIOPHARMACEUTICALS Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201 Patentee after: Bohui Biopharmaceutical Co.,Ltd. Address before: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201 Patentee before: ZHONGKE BIOPHARM CO.,LTD. |
|
| CP01 | Change in the name or title of a patent holder |