CN101802220B - 分子冗余测序法 - Google Patents

分子冗余测序法 Download PDF

Info

Publication number
CN101802220B
CN101802220B CN200880100371.XA CN200880100371A CN101802220B CN 101802220 B CN101802220 B CN 101802220B CN 200880100371 A CN200880100371 A CN 200880100371A CN 101802220 B CN101802220 B CN 101802220B
Authority
CN
China
Prior art keywords
sequence
nucleic acid
template
registration
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN200880100371.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN101802220A (zh
Inventor
F·克里斯蒂安
S·特纳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pacific Biosciences of California Inc
Original Assignee
Pacific Biosciences of California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences of California Inc filed Critical Pacific Biosciences of California Inc
Publication of CN101802220A publication Critical patent/CN101802220A/zh
Application granted granted Critical
Publication of CN101802220B publication Critical patent/CN101802220B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880100371.XA 2007-07-26 2008-07-25 分子冗余测序法 Active CN101802220B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96203607P 2007-07-26 2007-07-26
US60/962,036 2007-07-26
PCT/US2008/009064 WO2009017678A2 (en) 2007-07-26 2008-07-25 Molecular redundant sequencing

Publications (2)

Publication Number Publication Date
CN101802220A CN101802220A (zh) 2010-08-11
CN101802220B true CN101802220B (zh) 2013-07-31

Family

ID=40295742

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880100371.XA Active CN101802220B (zh) 2007-07-26 2008-07-25 分子冗余测序法

Country Status (7)

Country Link
US (4) US7901889B2 (https=)
EP (1) EP2183388A4 (https=)
JP (1) JP2010534474A (https=)
CN (1) CN101802220B (https=)
AU (1) AU2008282862B2 (https=)
CA (1) CA2693979A1 (https=)
WO (1) WO2009017678A2 (https=)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010534474A (ja) * 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子冗長配列決定
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
WO2009145820A2 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2274446B1 (en) * 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Two slow-step polymerase enzyme systems and methods
AU2009288696A1 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Sequencing by cognate sampling
US8921046B2 (en) * 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
CN104195227B (zh) 2008-11-07 2017-04-12 适应生物技术公司 通过序列分析监测状况的方法
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
EP2387627B1 (en) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunity profiling and methods for generation of monoclonal antibodies
AU2010206843B2 (en) * 2009-01-20 2015-01-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
CN102459643B (zh) 2009-06-25 2016-06-01 弗雷德哈钦森癌症研究中心 检测获得性免疫的方法
TWI439548B (zh) 2009-12-23 2014-06-01 Ind Tech Res Inst 序列校正方法與序列校正裝置
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
WO2011140433A2 (en) 2010-05-07 2011-11-10 The Board Of Trustees Of The Leland Stanford Junior University Measurement and comparison of immune diversity by high-throughput sequencing
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8986930B2 (en) 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
US9824179B2 (en) 2011-12-09 2017-11-21 Adaptive Biotechnologies Corp. Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP4372084A3 (en) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
DK2823060T3 (en) 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits
WO2013148400A1 (en) 2012-03-30 2013-10-03 Pacific Biosciences Of California, Inc. Methods and composition for sequencing modified nucleic acids
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CA2872468C (en) 2012-05-08 2020-10-27 Adaptive Biotechnologies Corporation Compositions and methods for measuring and calibrating amplification bias in multiplexed pcr reactions
US9116118B2 (en) 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
SG11201407888RA (en) * 2012-06-11 2014-12-30 Sequenta Inc Method of sequence determination using sequence tags
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US10777301B2 (en) 2012-07-13 2020-09-15 Pacific Biosciences For California, Inc. Hierarchical genome assembly method using single long insert library
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2014116729A2 (en) 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US10391467B2 (en) 2013-12-05 2019-08-27 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
US10385335B2 (en) 2013-12-05 2019-08-20 Centrillion Technology Holdings Corporation Modified surfaces
US10597715B2 (en) 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
EP3633047B1 (en) 2014-08-19 2022-12-28 Pacific Biosciences of California, Inc. Method of sequencing nucleic acids based on an enrichment of nucleic acids
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP4095261B1 (en) 2015-01-06 2025-05-28 Molecular Loop Biosciences, Inc. Screening for structural variants
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
CA2976580A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
WO2016154345A1 (en) 2015-03-24 2016-09-29 Pacific Biosciences Of California, Inc. Methods and compositions for single molecule composition loading
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
US20160326582A1 (en) * 2015-05-04 2016-11-10 Fry Laboratories, L.L.C. Method and kit for target molecule characterization
EP3527672B1 (en) 2015-06-09 2022-10-05 Centrillion Technology Holdings Corporation Oligonucleotide arrays for sequencing nucleic acids
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
EP3464612B8 (en) * 2016-05-31 2024-12-11 Switchback Systems, Inc. Two-color nanopore sequencing
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
WO2018017884A1 (en) 2016-07-20 2018-01-25 Genapsys, Inc. Systems and methods for nucleic acid sequencing
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
CN106388765B (zh) * 2016-11-09 2018-06-05 苏州微清医疗器械有限公司 超广角眼底成像系统
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
WO2019060628A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
AU2019392906A1 (en) 2018-12-07 2021-07-22 Octant, Inc. Systems for protein-protein interaction screening
EP3674702A1 (en) 2018-12-27 2020-07-01 Imec VZW Method for sequencing a polynucleotide using a biofet
WO2020243164A1 (en) 2019-05-28 2020-12-03 Octant, Inc. Transcriptional relay system
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
AU2021230282A1 (en) 2020-03-03 2022-09-22 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
JP7490071B2 (ja) 2020-03-11 2024-05-24 エフ. ホフマン-ラ ロシュ アーゲー シーケンシングのための新規核酸鋳型構造
EP4127225A1 (en) 2020-04-03 2023-02-08 F. Hoffmann-La Roche AG A method of detecting structural rearrangements in a genome
WO2021249825A1 (en) 2020-06-08 2021-12-16 F. Hoffmann-La Roche Ag Methods and compositions for detecting structural rearrangements in a genome
AU2021360589A1 (en) 2020-10-15 2023-06-22 Board Of Regents, The University Of Texas System Detection and analysis of structural variations in genomes
WO2022169767A1 (en) 2021-02-02 2022-08-11 Octant, Inc. Systems and methods for measuring cell signaling protein activity
WO2022208171A1 (en) 2021-03-31 2022-10-06 UCL Business Ltd. Methods for analyte detection
US12584107B2 (en) 2022-01-20 2026-03-24 3T Biosciences, Inc. T cell receptor (TCR) compositions and methods for optimizing antigen reactive T-cells
WO2024022207A1 (en) * 2022-07-25 2024-02-01 Mgi Tech Co., Ltd. Methods of in-solution positional co-barcoding for sequencing long dna molecules
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
WO2026019720A1 (en) 2024-07-15 2026-01-22 Pacific Biosciences Of California, Inc. Improved loading of molecules and complexes to reaction sites

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268267A (en) * 1987-08-21 1993-12-07 The General Hospital Corporation Method for diagnosing small cell carcinoma
WO1999007896A2 (en) * 1997-08-07 1999-02-18 Curagen Corporation Detection and confirmation of nucleic acid sequences by use of oligonucleotides comprising a subsequence hybridizing exactly to a known terminal sequence and a subsequence hybridizing to an unidentified sequence
US6312913B1 (en) * 2000-07-21 2001-11-06 Incyte Genomics, Inc. Method for isolating and characterizing nucleic acid sequences
WO2003074734A2 (en) * 2002-03-05 2003-09-12 Solexa Ltd. Methods for detecting genome-wide sequence variations associated with a phenotype

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2203023A (en) * 1938-11-17 1940-06-04 Servel Inc Refrigeration
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US4992280A (en) 1989-12-18 1991-02-12 Columbia Laboratories, Inc. Sustained flavorant release composition, method for making same and chewable structure incorporating same
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5470722A (en) 1993-05-06 1995-11-28 University Of Iowa Research Foundation Method for the amplification of unknown flanking DNA sequence
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
AU714486B2 (en) * 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
EP0910668A4 (en) * 1996-06-03 2003-02-19 Univ Alberta METHOD FOR DETECTING REASSEMBLED DNA
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
FI103809B1 (fi) * 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
EP2267165B1 (en) 1997-07-28 2016-11-30 Gen-Probe Incorporated Nucleic acid sequence analysis
US6071729A (en) * 1998-04-02 2000-06-06 Jeffries; Thomas W. Disruption of the cytochrome C gene in xylose-fermenting yeast
AU3567099A (en) 1998-04-16 1999-11-01 Packard Bioscience Company Analysis of polynucleotide sequence
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
EP1114184A2 (en) 1998-09-15 2001-07-11 Yale University Molecular cloning using rolling circle amplification
WO2000036151A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A heterogeneous assay for pyrophosphate detection
DE60045917D1 (de) 1999-02-23 2011-06-16 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
WO2001040516A2 (en) * 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
AU2074101A (en) * 1999-12-08 2001-06-18 Millennium Pharmaceuticals, Inc. Compositions, kits, and methods for identification, assessment, prevention, and therapy of cervical cancer
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
EP1328805A4 (en) 2000-09-28 2007-10-03 Wisconsin Alumni Res Found SYSTEM AND METHOD FOR VALIDATION, ALIGNMENT AND RECLASSIFICATION OF ONE OR MORE GENETIC SEQUENCE CARDS USING AT LEAST ONE ORDERED RESTRICTION CARD
EP1368497A4 (en) * 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
AU2003201817A1 (en) * 2002-01-04 2003-07-24 Stanford University Telomere-encoding synthetic dna nanocircles, and their use for the elongation of telomere repeats
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2004071155A2 (en) 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
JP2004298018A (ja) 2003-03-28 2004-10-28 Olympus Corp プローブ固相化反応アレイによる核酸の分離回収法
WO2005080605A2 (en) * 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
WO2005092038A2 (en) * 2004-03-22 2005-10-06 The Johns Hopkins University Methods for the detection of nucleic acid differences
US20060024711A1 (en) * 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7315019B2 (en) * 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US20070141598A1 (en) * 2005-02-09 2007-06-21 Pacific Biosciences Of California, Inc. Nucleotide Compositions and Uses Thereof
WO2007070642A2 (en) 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
JP4989493B2 (ja) * 2006-01-20 2012-08-01 オリンパス株式会社 分子内プローブによる核酸配列の検出方法
US20090075252A1 (en) 2006-04-14 2009-03-19 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080009007A1 (en) * 2006-06-16 2008-01-10 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
WO2008069973A2 (en) * 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
JP2010534474A (ja) * 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子冗長配列決定

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268267A (en) * 1987-08-21 1993-12-07 The General Hospital Corporation Method for diagnosing small cell carcinoma
WO1999007896A2 (en) * 1997-08-07 1999-02-18 Curagen Corporation Detection and confirmation of nucleic acid sequences by use of oligonucleotides comprising a subsequence hybridizing exactly to a known terminal sequence and a subsequence hybridizing to an unidentified sequence
US6312913B1 (en) * 2000-07-21 2001-11-06 Incyte Genomics, Inc. Method for isolating and characterizing nucleic acid sequences
WO2003074734A2 (en) * 2002-03-05 2003-09-12 Solexa Ltd. Methods for detecting genome-wide sequence variations associated with a phenotype

Also Published As

Publication number Publication date
JP2010534474A (ja) 2010-11-11
WO2009017678A2 (en) 2009-02-05
US20110212436A1 (en) 2011-09-01
US8535882B2 (en) 2013-09-17
US20150376690A1 (en) 2015-12-31
WO2009017678A3 (en) 2009-04-16
EP2183388A4 (en) 2010-09-08
US9732383B2 (en) 2017-08-15
US9051611B2 (en) 2015-06-09
AU2008282862B2 (en) 2014-07-31
CA2693979A1 (en) 2009-02-05
CN101802220A (zh) 2010-08-11
US20090029385A1 (en) 2009-01-29
US20140017690A1 (en) 2014-01-16
EP2183388A2 (en) 2010-05-12
US7901889B2 (en) 2011-03-08
AU2008282862A1 (en) 2009-02-05

Similar Documents

Publication Publication Date Title
CN101802220B (zh) 分子冗余测序法
JP5280841B2 (ja) 分解核酸の分析用組成物及び方法
CN102952877A (zh) 检测α珠蛋白基因拷贝数的方法和系统
CN106906295B (zh) 数字pcr检测基因点突变方法及装置
CN102586456A (zh) 一种多重竞争性pcr检测拷贝数变异的方法
Janes Single-cell states versus single-cell atlases—two classes of heterogeneity that differ in meaning and method
CN109554444A (zh) 一种检测核酸突变顺反式结构的方法及其试剂盒
Littlewood Platyhelminth systematics and the emergence of new characters
CN101487051A (zh) Braf基因突变的检测探针、液相芯片及其检测方法
Llora-Batlle et al. 10x Genomics Gene Expression Flex is a powerful tool for single-cell transcriptomics of xenograft models
Pisapia et al. The relevance of the reference range for EGFR testing in non-small cell lung cancer patients
WO1994002647A1 (en) Dna sizing control standards for electrophoretic analyses
CN109593832A (zh) 一种ARMS-ddPCR基因点突变的检测方法
CN109852697A (zh) 腺鳞癌诊断的分子靶点及其应用
Kinsler et al. SpaceBar enables single-cell-resolution clone tracing with imaging-based spatial transcriptomics
Singh et al. High-resolution voxelation mapping of human and rodent brain gene expression
CN107604067A (zh) 一种用于检测目的基因低频突变的引物及试剂盒
CN117625801B (zh) 16个Mini Y-STR的复合扩增体系及试剂盒
Kinsler et al. SpaceBar enables clone tracing in spatial transcriptomic data
CN117604103A (zh) 引物和探针组合物、试剂盒及在检测ikzf1基因缺失中的应用
Lee De novo gene expression reconstruction in space
CN112063694B (zh) 一种rna a-i编辑的酶识别检测方法
KR102421257B1 (ko) Lettuce big-vein associated virus를 특이적으로 검출하기 위한 프라이머 세트 및 이의 용도
CN118995962B (zh) 检测黄颡鱼的rpa引物对、探针和试剂盒及检测方法
Zhao et al. Emerging Challenges in Molecular Paleontology: Misapplication of Environmental DNA Fragments and Misconception of Deamination as a Key Criterion for In Situ DNA Identification

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant