The invention provides the active agent combinations/composition that reaches described target at least in some aspects.
Surprisingly, have now found that, bond of the present invention not only makes the action spectrum of phytopathogen to be prevented and treated and/or microorganism and/or insect be able to the additive property raising, can be from the sphere of action of two aspects expansion compound (A), compound (B) and compound (C) but also produced synergistic effect.At first, reduce the rate of application of compound (A), compound (B) and compound (C), kept same good effect simultaneously.Secondly, even when these three kinds of independent compounds are in low invalid fully rate of application scope, described bond still can obtain higher phytopathogen control efficiency.This makes on the one hand, has significantly widened the pedigree of the phytopathogen that can prevent and treat, on the other hand, has strengthened safety in utilization.
Yet except the actual synergy aspect sterilization and insecticidal activity, Pesticidal combinations of the present invention also has other surprising advantageous property that more broadly also can be described as synergistic activity.The example of the described advantageous property that can mention has: at other phytopathogen and/or microorganism and/or insect---for example at resistant strain---sterilization of widening and insecticidal activity spectrum; The active component rate of application that reduces; By the enough control of the present composition, even under the invalid fully rate of application of independent compound, also be like this to nuisance; During preparation or the advantageous property when using, for example in grinding, screening, emulsification, dissolving or divide the advantageous property of timing; The storage stability that improves; Improved photostability; More favourable degradability; Improved toxicological properties or ecological toxicology performance; The characteristic of improved useful plants, comprise: emergence rate (emergence), crop yield, more flourishing root system, tiller that (tillering) increases, plant height increases, blade is bigger, the basal leaf lethality is lower, it is stronger to tiller,, the required fertilizer of leaf look greener still less, required seed still less, tiller more voluminous, bloom more early, kernel maturity early, plant still less lodge (verse, lodginig), shoot growth improves, improved plant growing way and rudiment early; Other advantage arbitrarily perhaps well known to those skilled in the art.
Bond of the present invention also provides the interior absorption (systemicity) of improvement for used reactive compound.In fact, even some in the used Fungicidal compounds do not have any interior absorption or gratifying interior absorption, these compounds also can show a kind of like this performance in the present composition.
Similarly, bond of the present invention can improve the persistence of the sterilization drug effect of the reactive compound of using.
Also find, with in conjunction with or use the compound (A) that carries out of mode, compound (B) and compound (C) time separately, for independent individualized compound (A), compound (B) and compound (C) or aforementioned binary mixture (WO 05/009131), can more effective antagonism phytopathogen and/or microorganism and/or insect.
Another advantage of bond of the present invention is the drug effect that can obtain to improve.
Therefore, the invention provides a kind of like this bond, it comprises:
(A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide
With
(B) Imidacloprid; With
(C) another is selected from the reactive compound of following sterilization and insecticidal active compound: second worm nitrile, fluorine worm nitrile, thiophene worm quinoline, pleocidin, thiophene fluorine bacterium amine, (S)-3-chloro-N
1-2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl) ethyl] phenyl }-N
2-(1-methyl-2-methyl sulphonyl ethyl) phthalic amide and N-[2-(1, the 3-dimethylbutyl) phenyl]-5-fluoro-1,3-dimethyl-1H-pyrazole-4-carboxamide.
Compound (B) and most array (C) compound are referring to agricultural chemicals handbook (Pesticide Manual) the 13rd edition, 2003.Compound (C) is listed with common name.If there is not available common name before the application's priority date, then compound (C) is listed with the IUPAC name.
Preferred bond is listed below:
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) second worm nitrile,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) fluorine worm nitrile,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) thiophene worm quinoline,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) pleocidin,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) thiophene fluorine bacterium amine,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) (S)-3-chloro-N
1-2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl) ethyl] phenyl }-N
2-(1-methyl-2-methyl sulphonyl ethyl) phthalic amide,
● compound (A) 3,4-two chloro-N-(2-cyano-phenyl)-5-isothiazole formamide and compound (B) Imidacloprid and compound (C) N-[2-(1, the 3-dimethylbutyl) phenyl]-5-fluoro-1,3-dimethyl-1H-pyrazole-4-carboxamide.
The weight ratio of active compound component is chosen as the effect that can provide required, for example synergy.Generally, described weight ratio changes according to concrete reactive compound.Usually, the weight ratio between any two kinds of compounds is 100: 1 to 1: 100 independently of one another, preferred 75: 1 to 1: 75, and more preferably 50: 1 to 1: 50, and most preferably 25: 1 to 1: 25.Other part by weight between the adoptable any two kinds of compounds of the present invention is 250: 1 to 1: 250,200: 1 to 1: 200,150: 1 to 1: 150 independently of one another, and the preference degree of described ratio strengthens successively by given order.
Other weight ratio according to the present invention between spendable any two kinds of compounds is 90: 1 to 1: 90,80: 1 to 1: 80,70: 1 to 1: 70,60: 1 to 1: 60,40: 1 to 1: 40,30: 1 to 1: 30,10: 1 to 1: 10,5: 1 to 1: 5,4: 1 to 1: 4,3: 1 to 1: 3 independently of one another, and the preference degree of described ratio strengthens successively by given order.
When compound (A), compound (B) and compound (C) can exist with tautomeric form, described compound was interpreted as also comprising corresponding tautomeric form under situation about being suitable in context, even they are not mentioned respectively one by one.
Compound (A), compound (B) and compound (C) with at least one basic center can generate for example acid-addition salts with for example following various acid: strong inorganic acid, and inorganic acid for example is as perchloric acid, sulfuric acid, nitric acid, nitrous acid, phosphoric acid or halogen acids; Strong organic carboxyl acid, for example (as the halo) C that does not replace or replace
1-C
4Alkanecarboxylic acid such as acetate, saturated or unsaturated dicarboxylic acids such as ethanedioic acid, malonic acid, succinic acid, cis-1, fumaric acid and phthalic acid, hydroxycarboxylic acid such as ascorbic acid, lactic acid, malic acid, tartaric acid, citric acid or benzoic acid; Or organic sulfonic acid, as (as halo) C unsubstituted or that replace
1-C
4Alkane sulfonic acid or aryl sulfonic acid, for example methanesulfonic acid or p-methyl benzenesulfonic acid.Compound (A), compound (B) and compound (C) with at least one acid groups can for example generate salt, for example slaine, for example alkali metal salt or alkali salt, for example sodium salt, sylvite or magnesium salts with alkali; Perhaps generate salt with ammonia or organic amine, described organic amine is morpholine, piperidines, pyrrolidines, single lower alkanols alkyl amine, two lower alkanols alkyl amine or three lower alkanols alkyl amine (for example ethamine, diethylamine, triethylamine or dimethyl propyl amine) or monohydroxy lower alkanols alkyl amine, two hydroxyl lower alkanols alkyl amine or trihydroxy lower alkanols alkyl amine (for example MEA, diethanol amine or triethanolamine) for example.In addition, can choose the corresponding inner salt of formation wantonly.In the present invention, favourable salt on the preferred agrochemicals.In view of the free form of compound (A), compound (B) and compound (C) and the close relation between its salt form, under suitable and favourable situation, any when mentioning free compound (A), compound (B) and compound (C) or their salt in the context, be interpreted as also comprising respectively corresponding salt or free compound (A), compound (B) and compound (C).This equally also is applicable to the dynamic isomer and the salt thereof of compound (A), compound (B) and compound (C).
The various combinations of the term among the present invention " bond " expression compound (A), compound (B) and compound (C), for example combination of following form: single " premix (ready-mix) " form; The form of the Spray Mixing thing of the combination that constitutes by the independent preparation of single-activity compound, for example " bucket mixes "; And the independent form that is used in combination during the active component sequential application, sequential application is promptly for example used in several hours or several days one by one in the rationally short time.Preferably, the order of administration of compound (A), compound (B) and compound (C) is not necessary to practice the present invention.
Term among the present invention " pathogene " expression is to plant or hurtful all organisms of any plant part.
All fungi organisms and false bacterium organism (chromista organism) represented in term among the present invention " fungi ".
Term among the present invention " phytopathogen " expression is to plant or hurtful all fungi organisms of any plant part and false bacterium organism.Classification of fungi group's example has sac fungi (Ascomycota), basidiomycetes (Basidiomycota), chytrid (Chytridiomycota), imperfect fungi (Deuteromycota), polymerization bacterium (Glomeromycota), microsporidian (Microsporidia), zygomycete (Zygomycota) and imperfect fungus (anamorphicfungi).The example of false bacterium is oomycetes (Oomycota).
All bacterium organisms and protozoa organism represented in term among the present invention " microorganism ".Example is plasmodiophora brassicae (Plasmodiophoromycetes).
Term among the present invention " virus " expression is to plant or hurtful all viruses of any plant part.Example is DNA-, RNA and DNA and RNA retrovirus and subviral agent.
Term among the present invention " insect " expression is to plant or hurtful all aschelminths (aschelminthes) organism of any plant part and general arthropods (panarthropoda) organism.Example is Nematoda (Nematoda), Arthropoda (Arthopoda), Hexapoda (Hexapoda) and arachnids (Arachnida).
Described reactive compound has strong microbiocidal activity, bactericidal activity and insecticidal activity in the present composition; and can be used for preventing and treating undesirable plant pathogen and/or microorganism and/or insect in crop protection, material protection, veterinary field, the herding, and can be used as domestic pesticide.
In composition of the present invention, Fungicidal compounds can be used in the crop protection, for example to prevent and treat phytopathogen and/or microorganism and/or insect, as plasmodiophora brassicae, oomycetes, chytrid, zygomycete, sac fungi, basidiomycetes and imperfect fungi.
In composition of the present invention, pesticide compound can be used in the crop protection, for example to prevent and treat the pathogenic insect.
In composition of the present invention; killing the bacterium compound can be used in the crop protection; for example to prevent and treat microorganism, as pseudomonas (Pseudomonadaceae), rhizobium (Rhizobiaceae), enterobacteria (Enterobacteriaceae), excellent bacillus (Corynebacteriaceae) and streptomycete (Streptomycetaceae).
Sterilization and disinsection bond of the present invention and/or composition can be used for therapeutic or prophylactically prevent and treat phytopathogen and/or microorganism and/or the insect of plant or crop.Therefore, another aspect of the present invention, a kind of therapeutic is provided or has has prophylactically prevented and treated the phytopathogen of plant or crop and/or the method for microorganism and/or insect, this method comprises uses a kind of fungicide insecticide composition of the present invention in the following manner: it is applied to the soil that seed, plant or fruit or plant growing maybe will be grown.
Can handle all plants and plant part according to the present invention.Described plant is meant all plants and plant population, for example need with unwanted wild plant, cultivated species (comprising natural cultivated species) and botanical variety (no matter whether being subjected to botanical variety or plant breeder to weigh protection).Cultivated species and botanical variety can be the plants that comprises genetically modified plants that obtains by routine breeding and method of breeding, described conventional breeding and method of breeding can be aided with or replenish with one or more biotechnology methods, for example by using double haploid, protoplast fusion, random mutagenesis and directed mutagenesis, molecular labeling or genetic marker, perhaps by biotechnology and genetic engineering method.
Described plant part is meant all ground and under ground portion and organs of plant, and for example bud, leaf, Hua Hegen list for example leaf, needle, stem, branch, flower, fruit body, fruit and seed and root, bulb and rhizome in this regard.Crop and asexual and sexual propagation material are for example transplanted, bulb, rhizome, sarment and seed, also belong to plant part.
Term among the present invention " plant propagation material " expression can be used for all vegetable materials in the asexual or sexual propagation of plant.The example of plant propagation material is transplanted, bulb, rhizome, sarment, seed, fruit, grain, beanpod, fruit body, stem tuber and seedling.
Bond/the composition of the present invention that is used for crop protection opposing phytopathogen and/or microorganism and/or insect contains effectively but the reactive compound of the present invention of the phytotoxic amount of tool not." effectively but not the phytotoxic amount of tool " is defined as a kind of like this amount of bond of the present invention, and it is enough to prevent and treat satisfactorily or eliminate fully the mushroom disease of plant on the one hand and can cause any significant phytotoxicity symptom on the other hand.Described effective dose can change in a big way at one usually.This dosage depends on a plurality of factors, for example at fungi, plant, weather conditions, and the reactive compound in the bond of the present invention.
In the plant that can protect by the inventive method, the main field crops that can mention has, corn for example, soybean, cotton, rape oilseeds (Brassica oilseeds, cabbage type rape (Brassica napus)---for example Canadian rape (canola) for example, turnip (Brassicarapa), leaf mustard (B.juncea)---leaf mustard (mustard) for example, and brassicacarinata (Brassica carinata)), rice, wheat, beet, sugarcane, oat, rye, barley, grain, triticale, flax, liane and various plant classification groups' various fruit and vegetables, for example the rose family (for example belongs to kind of (Rosaceae sp.), a kind of fruit, such as apple, pear, etc. such as apple and pears, and drupe such as apricot, cherry, almond and peach, berry is strawberry for example), Ribesioidae sp., Juglandaceae belongs to kind of (a Juglandaceae sp.), Betulaceae belongs to kind of (a Betulaceae sp.), Anacardiaceae belongs to kind of (an Anacardiaceae sp.), Fagaceae belongs to kind of (a Fagaceae sp.), Moraceae belongs to kind of (a Moraceae sp.), Oleaceae belongs to kind of (an Oleaceae sp.), Actinidiaceae belongs to kind of (an Actinidaceae sp.), Lauraceae belongs to kind of (a Lauraceae sp.), Musaceae belongs to kind of (Musaceaesp.) (for example banana tree and plantation), Rubiaceae belongs to kind of (Rubiaceae sp.) (a for example coffee), Theaceae belongs to kind of (a Theaceae sp.), Sterculiaceae belongs to kind of (a Sterculiceae sp.), Rutaceae belongs to kind of (a Rutaceae sp.) (lemon for example, orange and grapefruit); Solanaceae belongs to kind of (a Solanaceae sp.) (tomato for example, potato, pepper, eggplant, colea), Liliaceae belongs to kind of (a Liliaceae sp.), composite family belongs to kind of (a Compositiae sp.) (lettuce for example, arithoke and witloof---comprise root witloof (root chicory), corn sow thistle (endive) or common witloof (common chicory)), Umbelliferae belongs to kind of (a Umbelliferae sp.) (carrot for example, parsley, celery and celeriac), Curcurbitaceae belong to kind of (Cucurbitaceaesp.) (cucumber for example---comprise pickled with cucumber (pickling cucumber), pumpkin, watermelon, cucurbit and muskmelon), green onion section belongs to kind of (Alliaceae sp.) (for example an onion and a leek), Cruciferae belongs to kind of (a Cruciferae sp.) (white cabbage for example, red cabbage, broccoli, cauliflower, brussels sprouts, a variety of Chinese cabbage, kohlrabi, radish (radish), horseradish, Chinese celery, Chinese cabbage), pulse family belongs to kind of (a Leguminosae sp.) (peanut for example, pea and Kidney bean---as climning bean and broad bean), Chenopodiaceae belongs to kind of (a Chenopodiaceae sp.) (fodder beet for example, foliage heet, spinach, table beet), composite family belongs to kind of (Asteraceae sp.) (a for example sunflower), Cruciferae (Brassicaceae sp.) (white cabbage for example, red cabbage, broccoli, cauliflower, brussels sprouts, a variety of Chinese cabbage, kohlrabi, radish and Canadian rape, rape, leaf mustard, horseradish and Chinese celery), pulse family (Fabacae sp.) (for example peanut and beans), Papilionaceae belongs to kind of (Papilionaceae sp.) (a for example soybean), Malvaceae (Malvaceae) (for example gumbo), Asparagaceae (Asparagaceae) (for example asparagus); Gardening and forest crop; Ornamental plants; And the homologue of the genetic modification of described crop.
Processing method of the present invention can be used for handling genetically modified organisms (GMO), for example plant or seed.Genetically modified plant (or genetically modified plants) is that heterologous gene stably is integrated into genomic plant.Such gene that provides or assemble in the plant outside mainly is provided described word " heterologous gene ", in the time of in this gene is introduced nuclear, chloroplast or mitochondrial genomes by express the protein paid close attention to or polypeptide or by downward modulation or silence one or more be present in endophytic other gene (for example use antisense technology, suppress technology or RNA interference-RNAi technology altogether) thus give the transformed gene plant new or improved agronomy or other characteristic.The heterologous gene that is positioned at genome is also referred to as transgenosis.The transgenosis that defines according to its particular location in Plant Genome is called as transformation plant or transgenic line (transformation or transgenic event).
According to plant species or plant cultivars, its location and growth conditions (soil, weather, nutrient plant vegetative period, nutrition), processing of the present invention also can produce superadditivity (" working in coordination with ") effect.Can obtain the following effect that surpasses actual desired thus: for example can reduce the rate of application of reactive compound used according to the invention and composition and/or widen its activity profile and/or improve its activity, improve plant growing, improve high temperature or cold tolerance, raising is to arid or to the tolerance of salt content in the water or in the soil, improve the quality of blooming, make gather simpler and easy, hasting of maturity, improve the productive rate of gathering, fruit is bigger, plant height is higher, the leaf look greener, bloom more early, improve the quality of the product of gathering and/or improve its nutritive value, improve the sugared concentration in the fruit, improve the bin stability and/or the processing characteristics of the product of gathering.
Under some rate of application, active agent combinations of the present invention also can have strengthening effect in the plant corpus.Therefore, they also are suitable for transferring the defense system of plant, to resist the invasion and attack of undesirable plant pathogen and/or microorganism and/or virus.Under suitable situation, this may be that bond of the present invention is resisted one of reason that the activity of fungi for example improves.In this article, the implication that plant is strengthened (induction of resistance) material is interpreted as the bond of a kind of like this material or material: the defense system that they can stimulating plant, thereby when inoculating undesirable plant pathogen and/or microorganism and/or virus and/or insect subsequently, treated plant shows significant resistance to described undesirable plant pathogen and/or microorganism and/or virus and/or insect.In the present invention, undesirable plant pathogen and/or microorganism and/or virus are interpreted as meaning phytopathogen, bacterium and virus and/or insect.Therefore, material of the present invention is used in and handles the invasion and attack that interior protective plant of regular period is afterwards resisted above-mentioned pathogene.Usually after handling plant, continue 1 to 10 day the period of realizing protection with reactive compound, preferably 1-7 days.
Preferred plant to be processed of the present invention and plant cultivars comprise all plants with genetic material of giving described plant (no matter it is to obtain by breeding and/or biological technique method) advantageous particularly, useful feature.
Preferred plant to be processed of the present invention and plant cultivars also can be coerced one or more biologies has resistance, and promptly described plant demonstrates at animal nuisance and microorganism nuisance---for example at nematode, insect, acarid, phytopathogen, bacterium, virus and/or viroids---defensive preferably.
The present invention plant to be processed and plant cultivars also can be those plants that one or more abiotic stress had resistance.The abiotic stress condition can comprise mineral exposure, ozone exposure, the high light exposure of the soil salinity, the increase of for example arid, low temperature exposure, hot exposure, osmotic stress, waterflooding, increase, the availability of restricted nitrogen nutrient, restricted phosphorus nutrient availability, shelter from heat or light and avoid.
The present invention plant to be processed and plant cultivars also can be those plants of the yield characteristics with raising.The productive rate of the raising of described plant can and be grown as the germination efficient of water-use efficiency, water maintenance efficient, improved nitrogen utilization, the carbon assimilation of raising, improved photosynthesis, raising and hasting of maturity by improved plant physiology function for example, growth and be produced.Productive rate also can be subjected to improved families of plant structure (plant architecture) (coerce and non-stress conditions under) influence, described improved families of plant structure includes but not limited to, early flowering, the seed number at the control of blooming, rice shoot vigor, plant size, internode number and distance, root growth, seed size, fruit size, pod size, pod number or spike number, each pod or the fringe of hybrid seed production, seed quality, the seed plumpness of raising, the seed dispersal of reduction, the pod cracking and the lodging resistance of reduction.Yield characteristics also comprises seed compositions, for example reduction of carbohydrate content, protein content, oil content and composition, nutritive value, anti-nutrient compounds, improved processing characteristics and stability for storage.
Have above-mentioned characteristic plant the non exhaustive property of example list in the Table A.
Table A
Crop |
Characteristic |
?GMO |
List of references |
All |
Water-use efficiency |
Be |
??WO2000/073475 |
All |
Use nitrogen efficient |
Be |
??WO1995/009911 |
All |
Use nitrogen efficient |
Be |
??WO1997/030163 |
All |
The photosynthesis that improves |
Be |
??WO2003/000066 |
Soybean |
Nematicide |
Not |
??WO1995/020669 |
Rape |
The pod cracking that reduces |
Be |
??WO2006/009649 |
Soybean |
Anti-aphid |
Not |
??WO2006/125065 |
Lettuce |
Anti-aphid |
Not |
??WO1997/046080 |
Rape |
Anti-Sclerotinia (Sclerotinia) |
Not |
??WO2006/135717 |
Crop |
Characteristic |
?GMO |
List of references |
Tomato |
Anti-Botrytis (Botrytis) |
Not |
??WO2006/046861 |
Lettuce |
Anti-dish downy mildew (Bremia) |
Not |
??US20070022496 |
Potato |
Anti-Ou Wenshi (Erwinia) |
Not |
??WO2004/049786 |
Cucumber |
Anti-long linear Tobamovirus (Closterovirus) |
Not |
??WO2002/022836 |
Capsicum |
Antiviral |
Not |
??WO2006/038794 |
The plant of handling according to the present invention can be the hybrid plant that gives expression to the hybrid vigour characteristic, and described characteristic can cause higher productive rate, vigor, health degree and usually to the resistance of biology and abiotic stress.Described plant can educate parent line (paternal line) hybridization and makes by a kind of selfing male sterile parent line (maternal side) and another kind of selfing are male usually.Hybrid seed is gathered from male sterile plants usually and is sold to the grower.Male sterile plants (for example corn) sometimes can make by the eunuchs fringe, and promptly machinery is removed male organ of multiplication (or male flower), and still, more generally, male sterility is obtained by the genetic determinant in the Plant Genome.In the case, especially when the product of wishing to gather from hybrid plant be kind of a period of the day from 11 p.m. to 1 a.m, guarantee hybrid plant male fertility recover normally useful fully.This can suitable can recover hybrid plant---contain and cause male sterile genetic determinant---[fertility of male fertility is realized by guaranteeing that paternal line has.The male sterile genetic determinant can be arranged in cytoplasm.The example of cytoplasmic male sterility (CMS) is described (WO 92/05251, WO95/09910, WO 98/27806, WO 05/002324, WO 06/021972 and US6,229,072) in for example Brassicas (Brassica species).But the male sterile genetic determinant also can be arranged in the nuclear gene group.Male sterile plants also can obtain by Plant Biotechnology rule such as genetic engineering.A kind of useful especially method that obtains male sterile plants has description in WO 89/10396, wherein for example ribalgilase such as barnase are optionally expressed in the tapetal cell of stamen.Then fertilizability can by ribonuclease inhibitor for example the expression of barstar in tapetal cell recover (for example WO 91/02069).
Plant or the plant cultivars (obtaining by Plant Biotechnology rule such as genetic engineering) handled according to the present invention can be the herbicide tolerant plant, i.e. the plant that one or more given weed killer herbicides are had tolerance.Described plant can obtain by genetic transformation or by the plant of selecting to contain the sudden change of giving described herbicide tolerant.
Herbicide resistant plants has for example glyphosate (glyphosate) tolerant plants, i.e. the plant that herbicide glyphosate or its esters are had tolerance.Can make plant have tolerance by diverse ways to glyphosate.For example, the glyphosate tolerant plant can be by with coding 5-enol pyruvoyl shikimic acid-3-phosphate synthase (5-enolpyruvylshikimate-3-phosphate synthase, gene-transformed plant EPSPS) and obtaining.The example of described EPSPS gene has AroA gene (mutant the CT7) (people such as Comai of salmonella typhimurium (Salmonella typhimurium) bacterium, 1983, Science 221,370-371), the CP4 gene of Agrobacterium kind (Agrobacterium sp.) bacterium (people such as Barry, 1992, Curr.Topics Plant Physiol., 7,139-145), the gene of coding petunia EPSPS (people such as Shah, 1986, Science 233,478-481), the gene of coding for tomato EPSPS (people such as Gasser, 1988, J.Biol.Chem., 263,4280-4289) or the gene (WO 01/66704) of coding Eleusine EPSPS.Also can be the EPSPS of the sudden change described in EP0837944, WO 00/66746, WO 00/66747 or the WO 02/26995 for example.The glyphosate tolerant plant also can obtain by the gene of expressing the coding glyphosate oxidoreductase, as U.S. Patent No. 5,776, and 760 and 5,463, described in 175.The glyphosate tolerant plant also can obtain by the gene of expressing the coding glyphosate acetyl transferase, for example described in WO02/36782, WO 03/092360, WO 05/012515 and the WO 07/024782.The glyphosate tolerant plant also can be by selecting to contain said gene the plant of spontaneous mutation obtain, for example described in WO 01/024615 or the WO 03/013226.
Other herbicide resistant plants has the plant that for example weed killer herbicide that suppresses glutamine synthase is had tolerance, and described weed killer herbicide is bialaphos (bialaphos), careless fourth phosphine (phosphinothricin) or careless ammonium phosphine (glufosinate) for example.Described plant can have the glutamine synthase mutant of resistance to obtain by the enzyme of this weed killer herbicide of expression detoxifcation or to inhibitory action.A kind of described enzyme (for example bar or the pat albumen of streptomycete (Streptomyces) kind) of effectively separating toxenzyme for the careless fourth phosphinothricin acetyl transferase of coding.The plant of expressing exogenous careless fourth phosphinothricin acetyl transferase is for example having description in the U.S. Patent No. 5,561,236,5,648,477,5,646,024,5,273,894,5,637,489,5,276,268,5,739,082,5,908,810 and 7,112,665.
Other herbicide tolerant plant also can be the plant that the weed killer herbicide that suppresses hydroxyphenylpyruvic acid dioxygenase (HPPD) is had tolerance.The hydroxyphenylpyruvic acid dioxygenase is the enzyme that catalysis ethyl-para-hydroxyphenyl ketone acid (HPP) changes into the reaction of alcapton.The HPPD inhibitor is had tolerance the naturally occurring resistance HPPD enzyme of plant available code gene or transform with the gene of the HPPD enzyme of encoding mutant, described in WO 96/38567, WO 99/24585 and WO99/24586.Obtain although also can transform by gene pairs plant inhibited with some HPPD inhibitor of coding but still that can form the enzyme of alcapton to natural HPPD enzyme to the tolerance of HPPD inhibitor.Described plant and gene have description in WO 99/34008 and WO02/36787.Plant, also can be improved by the gene-transformed plant with the prephenate dehydrogenase of encoding, described in WO 2004/024928 except that the gene with coding HPPD tolerance enzyme the tolerance of HPPD inhibitor.
Other herbicide tolerant plant also has the plant that acetolactate synthase (ALS) inhibitor is had tolerance.Known ALS inhibitor comprises, for example sulfonylureas, imidazolone, triazolo pyrimidine class, pyrimidine radicals oxygen (sulphur) yl benzoic acid ester class (pyrimidinyoxy (thio) benzoates) and/or Herbicidal sulphonylamino carbonyl triazole quinoline ketone (sulfonylaminocarbonyltriazolinone) weed killer herbicide.Known ALS enzyme (is also referred to as acetohydroxy acid synthase, AHAS) different weed killer herbicides and weed killer herbicide group tolerance are given in the sudden change of difference in, as for example in Tranel and Wright (2002, WeedScience 50:700-712) and U.S. Patent No. 5,605,011,5,378,824,5,141,870 and 5,013, described in 659.The preparation of sulfonylureas tolerant plants and imidazolinone-tolerant plant is in U.S. Patent No. 5,605, and 011,5,013,659,5,141,870,5,767,361,5,731,180,5,304,732,4,761,373,5,331,107,5,928,937 and 5,378,824; And be described among the international publication text WO 96/33270.Other imidazolinone-tolerant plant for example also is being described among WO 2004/040012, WO 2004/106529, WO 2005/020673, WO 2005/093093, WO 2006/007373, WO 2006/015376, WO 2006/024351 and the WO2006/060634.Other sulfonylureas tolerant plants and imidazolinone-tolerant plant also for example are being described among the WO 07/024782.
Other plant that imidazolone and/or sulfonylureas are had a tolerance can carry out that cell culture is selected or mutation breeding obtains by mutagenesis, under the situation that weed killer herbicide exists, for example United States Patent (USP) 5,084, in 082 among soybean, the WO97/41218 to rice, United States Patent (USP) 5,773,702 and WO99/057965 in to the description among lettuce or the WO01/065922 sunflower being carried out in beet, the United States Patent (USP) 5,198,599.
Plant or the plant cultivars (obtaining by Plant Biotechnology rule such as genetic engineering) handled according to the present invention also can be the genetically modified plants with insect-resistant, promptly the invasion and attack of some targeted insect are had the plant of resistance.Described plant can obtain by genetic transformation or by the plant of selecting to contain the sudden change of giving described insect-resistant.
" genetically modified plants with insect-resistant " used herein comprise and contain at least a genetically modified any plant that comprises the coded sequence of the following albumen of encoding:
1) insecticidal crystal protein of bacillus thuringiensis,Bt (Bacillus thuringiensis) or its desinsection part, people (1998 such as Crickmore for example, Microbiology and MolecularBiology Reviews, 62:807-813) in the thuringiensis bacillus toxin name, list, the insecticidal crystal protein that is upgraded by people such as Crickmore (2005) is (online: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/) or its desinsection part, for example Cry protide Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, the albumen of Cry3Aa or Cry3Bb or its desinsection part; Perhaps
2) in the presence of the another kind of crystalline protein of bacillus thuringiensis,Bt or its part, have thuringiensis bacillus crystallin or its part of insecticidal activity, the binary toxin of forming by Cry34 and Cry35 crystalline protein (people such as Moellenbeck for example, 2001, Nat.Biotechnol.19,668-72; People such as Schnepf 2006, Applied Environm.Microbiol.71,1765-1774); Perhaps
3) contain the hybrid insecticidal proteins of part of the different insecticidal crystal proteins of bacillus thuringiensis,Bt, as above-mentioned 1) the hybrid or above-mentioned 2 of albumen) the hybrid of albumen, the Cry1A.105 albumen (WO 2007/027777) that makes by MON98034 corn plants system for example; Perhaps
4) above-mentioned A1)-A3) any one, wherein for the scope of the targeted insect kind that obtains the higher insecticidal activity of targeted insect kind and/or expansion are acted on and/or owing in clone or conversion process, introduce change in the coding DNA with the albumen of some amino acid, a particularly 1-10 amino acid with another kind of aminoacid replacement, MON863 or MON88017 corn plants Cry3Bb1 albumen or the Cry3A albumen of MIR604 corn plants in being in being for example;
5) the desinsection secretory protein of bacillus thuringiensis,Bt or Bacillus cercus (Bacillus cereus) or its desinsection part, listed nutritive insecticidal albumen (VIP) in for example following network address: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html, for example albumen of VIP3Aa protide; Perhaps
6) in the presence of the another kind of secretory protein of bacillus thuringiensis,Bt or Bacillus cercus, have the bacillus thuringiensis,Bt of insecticidal activity or the secretory protein of Bacillus cercus, for example the binary toxin of forming by VIP1A and VIP2A albumen (WO 94/21795); Perhaps
7) contain hybrid insecticidal proteins, for example above-mentioned 1 from the part of the different secretory proteins of bacillus thuringiensis,Bt or Bacillus cercus) the hybrid or above-mentioned 2 of albumen) the hybrid of albumen; Perhaps
8) above-mentioned 1)-3) any one, wherein for the scope of the targeted insect kind that obtains the higher insecticidal activity of targeted insect kind and/or expansion are acted on and/or owing in clone or conversion process, introduce change in the coding DNA (simultaneously still encoding insecticidal proteins) with the albumen of some amino acid, a particularly 1-10 amino acid with another kind of aminoacid replacement, the VIP3Aa albumen of COT102 corn plants in being for example.
Certainly, genetically modified plants with insect-resistant used herein also comprise any plant that contains the combination of the gene of the albumen of any one in the above-mentioned 1-8 class of coding.In one embodiment, plant with insect-resistant comprises the transgenosis more than the albumen of any one in the above-mentioned 1-8 class of a kind of coding, thereby expands the scope of the targeted insect kind that is acted on when the different albumen that uses at the different target caste; Perhaps the same target caste had insecticidal activity but generation that the have the different modes of action different albumen of---for example being bonded to the different receptor binding sites of insect---come the insect-resistant of delay plant by using.
Plant or the plant cultivars (obtaining by Plant Biotechnology rule such as genetic engineering) handled according to the present invention also can have tolerance to abiotic stress.Described plant can obtain by genetic transformation or by the plant of selecting to contain the sudden change that can give described stress resistance.Useful especially stress tolerant plants comprises:
A. contain the genetically modified plant that can reduce polyadenous glycosides diphosphonic acid-ribose polymerase (PARP) expression of gene in plant cell or the plant and/or its activity, described in WO 00/04173 or EP 04077984.5 or EP 06009836.5.
B. contain the expression that can reduce PARG encoding gene in plant or the plant cell that improves stress tolerance and/or the genetically modified plant of its activity, for example described in the WO 2004/090140.
C. contain the genetically modified plant that the plant function enzyme to the NADH salvage route that improves stress tolerance is encoded, described plant function enzyme comprises nicotinamidase, nicotinic acid phosphoribosyl transferase, NAMN adenine transferase, NADH synzyme or nicotinamide phosphoribosyl transferase, for example described in EP04077624.7 or WO 2006/133827 or the PCT/EP07/002433.
The change that plant of handling according to the present invention or plant cultivars (obtaining by Plant Biotechnology rule such as genetic engineering) also can demonstrate quantity, quality and/or the bin stability of the product of gathering, and/or the change of the character of the concrete composition of the product of gathering, for example:
1) genetically modified plants of synthesis modification starch, the physico chemical property of this modified starch, particularly amylose content or amylose/amylopectin ratios, degree of branching, average chain length, side chain distribution, viscosity, gel strength, starch granularity and/or starch grain form, compare with the synthetic starch in wild-type plant cell or the plant change has taken place, thereby it can be more suitable for concrete application.The genetically modified plants of described synthesis modification starch are at for example EP 0571427, WO95/04826, EP 0719338, WO 96/15248, WO 96/19581, WO 96/27674, WO 97/11188, WO 97/26362, WO 97/32985, WO 97/42328, WO97/44472, WO 97/45545, WO 98/27212, WO 98/40503, WO99/58688, WO 99/58690, WO 99/58654, WO 00/08184, WO 00/08185, WO00/08175, WO 00/28052, WO 00/77229, WO 01/12782, WO 01/12826, WO 02/101059, WO 03/071860, WO 2004/056999, WO 2005/030942, WO 2005/030941, WO 2005/095632, WO 2005/095617, WO2005/095619, WO 2005/095618, WO 2005/123927, WO 2006/018319, WO 2006/103107, WO 2006/108702, WO 2007/009823, WO 00/22140, WO 2006/063862, WO 2006/072603, WO 02/034923, EP 06090134.5, EP 06090228.5, EP 06090227.7, EP 07090007.1, EP 07090009.7, WO 01/14569, WO 02/79410, WO 03/33540, WO 2004/078983, WO01/19975, WO 95/26407, WO 96/34968, WO 98/20145, WO 99/12950, WO 99/66050, WO 99/53072, US 6734341, WO 00/11192, WO98/22604, WO 98/32326, WO 01/98509, WO 01/98509, WO2005/002359, US 5824790, US 6013861, WO 94/04693, WO 94/09144, WO 94/11520, WO 95/35026, disclose among the WO 97/20936
2) synthetic non-starch carbohydrate polymer or the synthetic genetically modified plants of comparing the non-starch carbohydrate polymer of character with the wild-type plant that does not carry out genetic modification with change.Example has the plant of generation polyfructosan (polyfructose), especially inulin-type and levulan type polyfructosan, as open among EP 0663956, WO96/01904, WO96/21023, WO 98/39460 and the WO 99/24593; Produce α-1, the plant of 4-glucan is as open among WO 95/31553, US 2002031826, US 6284479, US 5712107, WO97/47806, WO 97/47807, WO 97/47808 and the WO 00/14249; Produce α-1,6 branched alpha-1, the plant of 4-glucan is as open among the WO 00/73422; Produce the plant of alternan, as open among WO 00/47727, EP 06077301.7, US 5908975 and the EP 0728213,
3) genetically modified plants of generation hyaluronan (hyaluronan) are as for example disclosing among WO2006/032538, WO 2007/039314, WO 2007/039315, WO 2007/039316, JP 2006304779 and the WO 2005/012529.
The plant that plant of handling according to the present invention or plant cultivars (can obtain by Plant Biotechnology rule such as genetic engineering) also can be the fiber properties with change, for example vegetable lamb.Described plant can obtain by the plant of genetic transformation or the sudden change by selecting to contain the fiber properties that can give described change, and described plant comprises:
A) contain the plant of the cellulose synthase gene that form changes, vegetable lamb for example is described in WO98/00549
B) contain the rsw2 that form changes or the plant of rsw3 homologous nucleic acid, vegetable lamb for example is described in WO2004/053219
C) have the plant that the enhanced sucrose phosphonate ester synthetase is expressed, vegetable lamb for example is described in WO01/17333
D) have the plant that the enhanced sucrose synzyme is expressed, vegetable lamb for example is described in WO02/45485
E) wherein the timing of fibrocyte base portion protoplasmic connection gate changes---for example by fiber selectivity β 1, the downward modulation of 3-dextranase---plant, vegetable lamb for example is described in WO2005/017157
F) have that reactive altered---for example by the expression of (comprising nodC and chitin synthetase gene) of N-acetyl-glucosamine transferase gene---plant of fiber, vegetable lamb for example is described in WO2006/136351
The plant that plant of handling according to the present invention or plant cultivars (can obtain by Plant Biotechnology rule such as genetic engineering) also can be the oil content Bu Tezheng with change, for example rape or relevant rape carex.Described plant can obtain by the plant of genetic transformation or the sudden change by selecting to contain the fiber properties that can give described change, and described plant comprises:
A) produce the plant of oil with high oleic acid content, rape plant for example, as for example US5,969,169, US 5,840,946 or US 6,323,392 or US 6,063, described in 947
B) produce the plant of oil with low linolenic content, rape plant for example, as US6,270828, US 6,169,190 or US 5,965, described in 755
C) produce the plant of the oil with low saturated fatty acid content, rape plant for example is as for example US patent 5,434, described in 283
The useful especially genetically modified plants that can handle according to the present invention be contain transformation plant or transformation plant combination and be the plant of the application theme of non-control state (non-regulated status), this application is to (the Animal and Plant Health Inspection Service of animals and plants sanitary inspection administration of United States Department of Agriculture (USDA) in the U.S., APHIS) propose, no matter whether described application has been authorized or is still undecided.Whenever all can locate from APHIS (4700River Road Riverdale, MD 20737, the U.S.)---for example from its website (URLhttp: //www.aphis.usda.gov/brs/not_reg.html) on---easily obtain this information.In the application's submission day, the non-control state application that the pendent or APHIS of APHIS authorizes is listed those among the table B, contains following information among the table B:
-application: the ID of application number.Can find technical description the independent application documents that---for example from the APHIS website---number obtain by this application from APHIS to transformation plant.These are described in this and include this paper in way of reference.
The prolongation (extension of petition) of-application: with reference to prolong request at original application.
-mechanism: the title of submitting the entity of this application to.
-be subjected to the control product: the corresponding plants kind.
-transgenosis phenotype: the proterties of giving plant by transformation plant
-transformation plant or transform system: non-control state request institute at strain be the title of (also be named as sometimes and be).
Various files of relevant this application that APHIS file: APHIS announces and can be to the various files of APHIS request.
Some other useful especially plant that contains single transformation plant or transformation plant combination is listed in the database of various countries or regional management organization (for example referring to http://gmoinfo.jrc.it/gmp_browse.aspx and http://www.agbios.com/dbase.php).
Other special genetically modified plants comprise containing and are in genetically modified plant agronomy neutrality or vantage point, described in an arbitrary piece of writing in the listed patent publication among the table C.
Plant species |
Strain system |
Feature |
References |
Corn |
??PV-ZMGT32??(NK603) |
Glyphosate tolerant |
??US?2007-056056 |
Corn |
??MIR604 |
Insect-resistant (cry3a055) |
??EP1737290 |
Corn |
??LY038 |
High-lysine content |
??US7157281 |
Corn |
??3272 |
From processing corn (α-Dian Fenmei) |
??US2006-230473 |
Corn |
??PV-ZMIR13??(MON863) |
Insect-resistant |
??US2006-095986 |
Corn |
??DAS-59122-7 |
Insect-resistant |
??US2006-070139 |
Corn |
??TC1507 |
Insect-resistant |
??US2005-039226 |
Corn |
??Mon810 |
Insect-resistant |
??US2004-180373 |
Corn |
??VIP1034 |
Insect-resistant |
??WO03052073 |
Corn |
??B16 |
Grass ammonium phosphine (glyfosinate) resistance |
??US2003126634 |
Corn |
??GA21 |
Glyphosate resistance |
??US6040497 |
Corn |
??GG25 |
Glyphosate resistance |
??US6040497 |
Corn |
??GJ11 |
Glyphosate resistance |
??US6040497 |
Corn |
??FI117 |
Glyphosate resistance |
??US6040497 |
Corn |
??GAT-ZM1 |
Glufosinate tolerant |
??WO01/51654 |
Plant species |
Strain system |
Feature |
References |
Wheat |
Strain is 1 |
Fusarium resistance (the mould toxin of single-ended spore (trichothecene) 3-O-transacetylase) |
??CA2561992 |
Rice |
??GAT-OS2 |
Glufosinate tolerant |
??WO01/83818 |
Have above-mentioned characteristic plant the non exhaustive property of example list in the Table A
The useful especially genetically modified plants that can handle according to the present invention are the plant that contains the combination of transformation plant or transformation plant, and described plant is listed in the database of various countries for example or regional management organization (referring to for example http://gmoinfo.jrc.it/gmp_browse.aspx and http://www.agbios.com/dbase.php).
The useful especially genetically modified plants that can handle according to the present invention are listed among the table B with its trade (brand) name.
Table B
Sequence number |
Trade (brand) name |
Example |
Describe |
??B-63 |
??YieldGard??Rootworm?Ready??2 |
Corn |
MON810 strain system+NK603 strain system, Cry1Ab |
??B-64 |
??YieldGard?VT??Pro |
Corn |
MON89034 strain system/Cry 1A.105+Cry 2Ab2 |
??B-65 |
??YieldGard?VT??Rootworm |
Corn |
MON88017 strain system/Cry3Bb1 |
??B-66 |
??YieldGard?VT??Triple |
Corn |
MON88017 strain system/Cry3Bb1+ Mon810 strain system, Cry1Ab |
??B-67 |
??YieldGard?VT??Triple?Pro |
Corn |
MON88017 strain system/Cry3Bb+MON89034 strain system/Cry1A.105+ Cry2Ab2 |
??B-68 |
??YieldMaker
TM |
Corn |
Comprise Roundup Ready 2 technology, YieldGard VT, YieldGard Corn Borer, YieldGard Rootworm and YieldGard Plus |
Some other special transgenosis rice plant comprises contains at least a genetically modified plant that is in the neutral or useful position of agronomy, as shown in table C.
Table C2
Strain system |
Describe |
CL121, CL141, CFX51 |
The tolerance to imidazolidinone weedicide Imazethapyr (imazethapyr) of inducing with ethylmethane sulfonate (EMS) mutagenesis acetolactate synthase (ALS). |
IMINTA-1 IMINTA-4 |
The tolerance to imidazolidinone weedicide of inducing with sodium azide mutagenesis acetolactate synthase (ALS). |
Strain system |
Describe |
LLRICE06 LLRICE62 |
The careless ammonium phosphine herbicide tolerant rice that the careless fourth phosphinothricin acetyl transferase (PAT) of the modification of the gene by inserting coding soil bacteria streptomyces hygroscopicus (Streptomyces hygroscopicus) is produced. |
LLRICE60 1 |
The careless ammonium phosphine herbicide tolerant rice that the careless fourth phosphinothricin acetyl transferase (PAT) of the modification of the gene by inserting coding soil bacteria streptomyces hygroscopicus is produced. |
PWC16 |
The tolerance to the imidazolidinone weedicide Imazethapyr of inducing with ethylmethane sulfonate (EMS) mutagenesis acetolactate synthase (ALS). |
In an extremely special embodiment, described phytopathogen of a kind of therapeutic or preventative control plant or crop and/or the method for microorganism and/or insect, this method comprises in the following manner and to use the bond of ((A) and (B)) or ((A) and (B) with (C)): it is applied to wherein activity expression mechanism corresponding to seed, plant propagation material, plant or the fruit of a kind of genetically modified plant among form A, B or the C.
In an extremely special embodiment, described phytopathogen of a kind of therapeutic or preventative control plant or crop and/or the method for microorganism and/or insect, this method comprises in the following manner and to use the bond of ((A) and (B)) or ((A) and (B) with (C)): it is applied to wherein activity expression mechanism corresponding to seed, plant propagation material, plant or the fruit of a kind of genetically modified plant among arbitrary form A, B or the C.
On the other hand, provide a kind of composition that contains bond of the present invention.Preferred this sterilization and Pesticidal combination contain agricultural and go up acceptable additive, solvent, carrier, surfactant or filler.
Natural or synthetic, the organic or inorganic compound of term among the present invention " carrier " expression, reactive compound (A), reactive compound (B) and reactive compound (C) combine with this compound or are easier to use when mixing, and particularly impose on plant part.Therefore the preferred inertia of this carrier and should be agricultural at least and go up acceptable.This carrier can be solid or liquid.
Suitable solid carrier is as follows:
Ammonium salt for example, with natural rock powder such as kaolin, clay, talcum, chalk, quartz, attapulgite, montmorillonite or diatomite, and the synthetic rock powder such as silica, aluminium oxide and the silicate of polymolecularity, petroleum paraffin, solid fertilizer, water, alcohols---preferred butanols, organic solvent, mineral oil and vegetable oil and derivative thereof;
The suitable solid carrier of granule has: for example pulverize and the natural rock of classification, as calcite, marble, float stone, sepiolite, dolomite and synthetic inorganic and organic dust particle; The particle of organic matter (for example paper, sawdust, coconut husk, corn stalk and tobacco rods);
The gaseous diluent or the carrier of liquefaction be meant and be the liquid of gaseous state at normal temperatures and pressures, and aerosol spray for example is as halogenated hydrocarbons and butane, propane, nitrogen and carbonic acid gas.
The adhesive that can use in preparation has; carboxymethyl cellulose for example, the polymer of natural and synthetic powder, particle or latex shape is as gum Arabic, polyvinyl alcohol, polyvinyl acetate; with natural phospholipid such as cephalin and lecithin, and synthetic phospholipid.Other additive can be mineral oil or the vegetable oil and the wax class of optional modification.
Suitable filler has, for example water, polarity and nonpolar organic chemistry liquid, for example be selected from amine, acid amides, lactam (for example N-alkyl pyrrolidone) and lactone, sulfone and the sulfoxide (for example dimethyl sulfoxide (DMSO)) of aromatic hydrocarbon and non-aromatic hydrocarbon (for example paraffinic, alkyl benzene, Fluhyzon, chlorobenzene), alcohols and polyalcohol (if suitable, it also can be that replace, etherificate and/or esterification), ketone (for example acetone, cyclohexanone), ester (comprising fats and oils) and (gathering) ether, unsubstituted and replacement.
If used filler is a water, also can use-case such as organic solvent make secondary solvent.The suitable liquid solvent mainly contains: aromatic species, for example dimethylbenzene, toluene or Fluhyzon, chlorinated aromatics and chlorination aliphatic hydrocarbon be chlorobenzene, vinyl chloride or carrene for example, aliphatic hydrocarbon is cyclohexane or paraffinic, for example petroleum distillate, mineral oil and vegetable oil for example, alcohols is butanols or ethylene glycol and ether and ester for example, ketone is acetone, MEK, methyl iso-butyl ketone (MIBK) or cyclohexanone for example, and intensive polar solvent is methyl-sulfoxide for example, and water.
The present composition also can contain additional compound.Particularly, said composition also can contain surfactant.Surfactant can be emulsifier, dispersant or wetting agent or the described surfactant mixtures of a kind of ionic or nonionic.That can mention has a for example polyacrylate; lignosulfonates; phenolsulfonate or naphthalene sulfonate; oxirane and fatty alcohol or with fatty acid or with the condensation polymer of fatty amine; the phenol (particularly alkylphenol or aryl phenol) that replaces; sulfosuccinate ester salt; taurine derivatives (particularly taurine Arrcostab); the phosphate of polyoxyethylenated alcohol or phenol; the fatty acid ester of polyalcohol; and contain sulfate radical; the derivative of the described compound of sulfonate radical and phosphate radical functional group, for example alkylaryl polyglycol ether; alkylsulfonate; alkyl sulfate; arylsulphonate; protein hydrolyzate; lignin sulfite waste liquor and methylcellulose.When reactive compound and/or inert carrier are water insoluble and when used carrier agent (vector agent) when being water, must there be at least a surfactant usually.Preferred surfactant content can account for 5 weight %-40 weight % of composition.
Suitable emulsifier and/or blowing agent have: for example nonionic and anion emulsifier, and as polyoxyethylene fatty acid ester, polyoxyethylene aliphatic alcohol ether.Suitable dispersant is nonionic and/or ionic species, for example is selected from pure POE and/or POP ether, acid and/or POP or POE ester, alkylaryl and/or POP or POE ether, fat and/or POP-POE adduct, POP and/or POE polyol derivative, POE and/or POP/ sorbitan or sugared adduct, alkyl or aryl sulphate, sulfonate and phosphate or corresponding PO ether adduct.In addition, suitable oligomer or polymer, for example based on vinyl monomer, acrylic acid, independent EO and/or PO or its with for example (gather) combining of alcohols or (gathering) amine.Also can use lignin and sulfonic acid thereof, cellulose, aromatic series and/or the aliphatic sulfonic acid of simple cellulose (simple cellulose) and modification and with the adduct of formaldehyde.Suitable dispersant is for example lignin sulfite waste liquor and methylcellulose.
Can use colouring agent, for example inorganic pigment such as iron oxide, titanium dioxide, Prussian blue (ferrocyanblue) and organic pigment such as alizarin dyes, azo dyes and metal phthalocyanine dyestuff reach trace element as iron, manganese, boron, copper, cobalt, molybdenum and zinc salt.
Randomly, also can comprise other additional compound, for example protective colloid, adhesive, thickener, thixotropic agent, bleeding agent, stabilizing agent, chelating agent.More generally, described reactive compound routinely preparation technique combine with any solid or liquid additive.
Usually, composition of the present invention can contain 0.05-99 weight %, preferred 1-70 weight %, the reactive compound of 10-50 weight % most preferably.
Bond of the present invention or composition can itself, the type of service of its dosage form or preparation thus uses, aerosol for example, microcapsule suspending agent, cold mist agent (cold foggingconcentrate), thermal fog, the micro-capsule granula, granula subtilis, the seed treatment suspending agent, ready to use solution, pulvis, missible oil, oil-in-water emulsion, water-in-oil emulsion, big granula, fine granule, the oil-dispersing property pulvis, oil suspending agent, finish, foaming agent (froths), paste, plant the clothing agent, colloidal suspending agent (suspending agent), outstanding newborn concentrating agents, aqueous solvent, suspending agent, soluble powder, granule, water-soluble granular formulation or tablet, the seed treatment water solube powder, wetting powder, natural and the synthetic material of dipping reactive compound, seed is with polymeric material and involucrum microcapsule formulations, and cold mist of ULV and hot mist preparation, (compression) gas, foaming agent, stylus, dry seeds is handled pulvis, seed treatment solution, the liquor of ultra low volume (ULV), the suspending agent of ultra low volume (ULV), water-dispersible granules or tablet, wet seed treatment water-dispersible powder.
Described preparation is by---for example conventional filler and solvent or thinner, emulsifier, dispersant and/or adhesive or fixative, wetting agent, waterproofing agent---mixes with a kind of known way being prepared with reactive compound or active agent combinations and conventional additives, if suitable, add drier and UV stabilizing agent, colouring agent, pigment, defoamer, preservative, secondary thickener (secondary thicknener), adhesive, gibberellin and water and other processing aid.
Described composition not only comprises by what suitable equipment (for example spraying or dusting device) was applied to pending plant or seed promptly uses composition, and comprises and be applied to the dense commercial composite that must dilute before the crop.
Behind the seedling to the control of the infringement phytopathogen of plant and/or microorganism and/or insect mainly by handling soil with crop protection agents and the plant shoot branch carries out.Consider that crop protection agents to the healthy issuable influence of environment and human and animal, just making great efforts to reduce the amount of the reactive compound of using.
Active agent combinations of the present invention can reach by the type of service conduct of described formulation preparation and the mixture use of other active compound its commercial preparation, and described other reactive compound is insecticide, attractant, chemosterilant, bactericide, miticide, nematocide, fungicide, growth regulatory substance, weed killer herbicide, safener, fertilizer or semiochemical for example.
With active agent combinations of the present invention the processing of plant and plant part is directly carried out with conventional treatment method or undertaken by acting on its environment, habitat or storage areas, described conventional treatment method is for example irrigated (perfusion), drip irrigation, spraying, vaporization (vaporizing), atomizing (atomizing), broadcasts sowing, dusts, foams, is coated with (spreading-on), is reached as dry seeds and handle pulvis, seed treatment solution, seed treatment water-soluble powder, wet seed treatment water-soluble powder; Or, under the situation of propagating materials, especially under the situation of seed, also can be undertaken by dry treatment, wet process, liquid handling, one or more layers dressing by crust.In addition, also can use reactive compound, perhaps active agent preparations or reactive compound itself be injected soil by the ultra low volume method.
The rate of application of described bond can change according to the concrete reactive compound in for example type of service, agrotype, the described bond, the type (if suitable) of plant propagation material, but should be able to make reactive compound in the described bond be able to provide the effective dose of the activity (for example disease or control of insect) of required enhancing, it can be determined by test.
Processing method of the present invention also provide compound (A) and (B) with simultaneously, separately or mode in succession use.
Conventional active compound doses/the rate of application that uses is usually and advantageously in the processing method of the present invention
-leaf surface treatment: 0.1 to 10,000g/ha (hectare), preferred 10 to 1,000g/ha, more preferably 25 to 300g/ha compound (A); 0.1 to 10,000g/ha, preferred 10 to 1,000g/ha, more preferably 50 to 300g/ha compound (B); 0.1 to 10,000g/ha, preferred 10 to 1,000g/ha, more preferably 50 to 300g/ha compound (C); Under perfusion or drip irrigation situation about using, especially when the use inert base for example rock wool or or during perlite, dosage even also can reduce;
-seed treatment: the compound (A) of every double centner seed 2 to 200g, preferred every double centner seed 3-150g; The compound (B) of every double centner seed 2 to 200g, preferred every double centner seed 3-150g; The compound (C) of every double centner seed 2 to 200g, preferred every double centner seed 3-150g;
-soil treatment: 0.1 to 10,000g/ha, preferred 1 to 5, the compound of 000g/ha (A); 0.1 to 10,000g/ha, preferred 1 to 5, the compound of 000g/ha (B); 0.1 to 10,000g/ha, preferred 1 to 5, the compound of 000g/ha (C).
Here pointed dosage only provides with the form of the illustrative examples of the inventive method.Those skilled in the art will know that how to adjust application dosage, particularly according to the kind of pending plant or crop.
Bond of the present invention can be used to protective plant opposing insect and/or phytopathogen and/or microorganism and/or insect in certain section time range of protecting after handling.The time range that protective effect comes into force after handling plant, continued 1-28 days, preferred 1-14 days usually with bond or treatment of plant propagation material after be up to 200 days.
Processing method of the present invention also can be used for handling propagating materials for example stem tuber or rhizome, and seed, seedling or sprigging thing and plant or phytograft.This processing method also can be used for handling root.Processing method of the present invention also can be used for handling plant shoot branch, for example trunk, stem or stem, leaf, flower and the fruit of the plant of paying close attention to.
Another aspect of the present invention is the method for the natural materials or their form processing of a kind of protective plant or animal origin, and described material is taken from the natural life circulation; This method comprises that the bond with a kind of compound (A), compound (B) and compound (C) is applied to the natural materials of described plant or animal origin or their form processing with cooperative effective quantity.
Preferred embodiment is the natural materials in a kind of protective plant source or the method for their form processing, and described material is taken from the natural life circulation; This method comprises that the bond with a kind of compound (A), compound (B) and compound (C) is applied to the natural materials of described plant origin or their form processing with cooperative effective quantity.
Further preferred embodiment is the method for a kind of protection fruit (preferred the operatic circle, drupe, berry and citrus fruit) or their form processing, and described fruit is taken from the natural life circulation; This method comprises that the bond with a kind of compound (A), compound (B) and compound (C) is applied to the natural materials of described plant origin or their form processing with cooperative effective quantity.
The present invention includes a kind of with compound (A), compound (B) and compound (C) while methods for the treatment of seed.The present invention also comprises a kind of with compound (A), compound (B) and compound (C) difference methods for the treatment of seed.
The present invention also comprises a kind of seed of having used compound (A), compound (B) and compound (C) to handle simultaneously.The present invention also comprises a kind of seed of having used compound (A), compound (B) and compound (C) to handle respectively.For a kind of seed in back, active ingredient can be applied in the discrete layer.These layers can be chosen wantonly by other layer separation that comprises or do not comprise active ingredient.
Bond of the present invention and/or composition are particularly suitable for handling seed.By insect and/or phytopathogen and/or microorganism and/or insect cause to the major part infringement of cultivated species by infecting lay up period seed and sowing go into during seed after underground and the plant germination and germinate after seed take place.Since just the root of growing plants and bud is responsive especially and or even a spot of infringement all can cause the withered of whole strain plant, therefore should the stage especially crucial.Therefore people to by use suitable medicament protection seed and germinate in plant have sizable interest.
By handle plant seed pest control and/or phytopathogen and/or microorganism and/or insect for people long-term known to and be the theme of updating.But, many problems that solve are unsatisfactorily always arranged in the processing of seed.Therefore, wish to develop the method for such protection seed and germination plant, this method makes needn't be after planting or use plant protection product behind the plant germination in addition.Also wish to optimize the amount of institute's applying active substances, the infringement that does not make plant originally experience the reactive compound of using so that protect the invasion and attack of seed and germination plant opposing insect and/or phytopathogen and/or microorganism and/or insect as well as possiblely.Particularly, methods for the treatment of seed also should be considered sterilization and the insecticidal properties that genetically modified plants are intrinsic, reaches the best protection of seed and germination plant with the minimal consumption with plant protection product.
Therefore, the invention particularly relates to a kind of method that infects of protecting seed and germination plant to avoid insect and/or phytopathogen and/or microorganism and/or insect, wherein said seed is with bond/compositions-treated of the present invention.In addition, the invention still further relates to bond/composition of the present invention and be used to handle the purposes of seed with protection seed and germination plant antagonism insect and/or phytopathogen and/or microorganism and/or insect.And, the invention still further relates to the seed of using bond/compositions-treated of the present invention to cross for the protection that obtains antagonism insect and/or phytopathogen and/or microorganism and/or insect.
One of advantage of the present invention is; because the distinctive internal absorption characteristic of bond/composition of the present invention; not only protect seed itself to avoid insect and/or phytopathogen and/or microorganism and/or insect infringement with the processing that these bond/compositions carry out, and the plant of being unearthed after the protection rudiment.Can save at seeding time in this way or thereafter soon to the cultivation thing direct processing.
Another advantage is, compare with each single reactive compound, the collaborative increase of the sterilization of bond/composition of the present invention and insecticidal activity, this activity surpass single all reactive compounds of using activity add and.Make that in this way the amount of optimizing the reactive compound of using becomes possibility.
What also can be looked at as advantage is, mixture of the present invention also can be used for particularly following genetically modified seed, wherein can express protein at insect and phytopathogen and/or microorganism and/or insect by the plant of this seed germination.By using the described seed of chemicals treatment of the present invention; some insect and/or phytopathogen and/or microorganism and/or insect can be prevented and treated by for example expressing insecticidal proteins; and surprisingly; the synergistic activity that uses medicament of the present invention to occur appending, this synergistic activity have further improved the effectiveness to the protective effect of insect invasion and attack.
Medicament of the present invention is suitable for protecting the seed of all types botanical variety on the books, it is used in agricultural, the greenhouse, in the forestry, bar excute a law in, in the gardening or the vineyard.Particularly, this is applicable to the seed of cereal (for example wheat, barley, rye, triticale, grain, oat, rice), corn, cotton, soybean, potato, sunflower, Kidney bean, coffee, beet (for example sugar beet, foliage heet and fodder beet), peanut, rape, rape seed, opium poppy, olive, coconut, cocoa, sugarcane or tobacco.Bond/composition of the present invention also is suitable for handling the seed of foregoing fruit plant and vegetables (for example tomato, cucumber, onion and lettuce), lawn, meadow and ornamental plants.Particularly importantly to the processing of the seed of wheat, barley, rye, triticale, oat, corn, rice, soybean, cotton, rape, rape seed.
As previously mentioned, with the processing particular importance of bond/composition of the present invention to transgenic seed.This is applicable to the plant seed that contains at least a following heterologous gene usually, and described heterologous gene control has the polypeptide expression of specific insecticidal properties.Heterologous gene in the transgenic seed can derive from microorganism for example bacillus (Bacillus), rhizobium (Rhizobium), pseudomonad (Pseudomonas), Serratia (Serratia), trichoderma (Trichoderma), Clavibacter, Paraglomus (Glomus) or Gliocladium (Gliocladium).The present invention is particularly suitable for handling the transgenic seed that contains at least a following heterologous gene, and described heterologous gene derives from Bacillus, and its gene outcome shows the activity to European corn borer and/or corn root leaf A (western corn rootworm).Particularly preferably be the heterologous gene that derives from bacillus thuringiensis,Bt.
In the context of the present invention, bond/composition of the present invention is applied to seed separately or with suitable formulation.Preferably stable as to handle seed under the state that is without prejudice during being enough to handling at seed.To the processing of seed usually can gather and sow between any time carry out.The conventional seed that uses is for separating and removed the seed of spadix, shell, stem, pod, fine hair or pulp from plant.The seed that uses is that gathered, that purify and is dried to the seed that moisture is lower than 15% w/w.Perhaps, also can use the seed of dry back with water treatment and then drying.
Usually must be noted that during seed treatment: the amount that is applied to the bond/composition of the present invention of seed and/or other additive should be chosen as can not be damaged the germination of seed and can not damage the plant that emerges.Especially to when using, showing the reactive compound of phytotoxic effect with certain amount.
Bond/composition of the present invention can directly be used, and does not promptly contain additional compound and not diluted.Usually preferably bond/composition of the present invention is applied to seed with the form of dosage forms.It is known and be described at for example following document for those skilled in the art to be used for the dosage forms of seed treatment and method: US 4,272,417A, US 4,245,432A, US 4,808,430A, US 5,876,739A, US 2003/0176428A1, WO2002/080675A1, WO 2002/028186A2.
Active agent combinations that can be used according to the invention and composition can change into conventional seed dressing preparation, for example solution, emulsion, suspending agent, pulvis, foaming agent, slurries or other seed coating material and ULV preparation.
Described preparation is by mixing preparation in a known way with reactive compound or active agent combinations, for example conventional filler of described conventional additives and solvent or thinner, colouring agent, wetting agent, dispersant, emulsifier, defoamer, preservative, secondary thickener, adhesive, gibberellin and optional water with conventional additives.
Can be present in the suitable colouring agent that the present invention dresses seed in the preparation and comprise that all routines are used for described purpose colouring agent.Both the pigment that is slightly soluble in water can be used, also water-soluble dyestuff can be used.The example that can mention comprises that known name is called the colouring agent of rhodamine B, C.I. pigment red 122 and C.I. solvent red 1.
Can be present in dress seed suitable wetting agent in the preparation of the present invention comprises and promotes the wetting and conventional all substances that are used for active farmingization substance preparation.Can preferably use Fluhyzon-sulfonate, for example diisopropyl or diisobutyl naphthalene-sulfonate.
Can be present in suitable dispersant and/or the emulsifier that the present invention dresses seed in the preparation and comprise all non-ionic, anionic and cationic dispersants of using always in the active farmingization substance preparation of summarizing above.
Can be present in dress seed suitable defoamer in the preparation of the present invention and be included in the material of all suppressed foams of routine in the active farmingization substance preparation.Can preferably use silicone defoaming agent and dolomol.
Can be present in the suitable preservatives that the present invention dresses seed in the preparation and be included as all substances that described purpose can be used in the farming composition.In the mode of example, that can mention has two chlorophenols and a phenmethylol hemiformal.
Can be present in the suitable secondary thickener that the present invention dresses seed in the preparation and be included as all substances that described purpose can be used in the farming composition.Preferred suitable clay that cellulose derivatives, acrylic acid derivative, xanthans, modification are arranged and polymolecularity silica.
Can be present in the suitable bonding that the present invention dresses seed in the preparation and be included in spendable all conventional adhesives in the seed seed dressing.That can preferably mention has polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and a tylose.
The suitable gibberellin that the present invention dresses seed in the preparation be can be present in and gibberellin A1, A3 (=gibberellic acid), A4 and A7 preferably included, preferred especially gibberellin A 3 (=gibberellic acid).The gibberellin of formula (II) is known, and the nomenclature of gibberellin can be at the following document of mentioning (referring to R.Wegler " Chemie der Pflanzenschutz-and
", the 2nd the volume, Springer Verlag, Berlin-Heidelberg-New York, 1970, the 401-412 pages or leaves) in find.
Comprise all mixing apparatus that can conventional be used to dress seed with seed dressing preparation of the present invention or by the suitable mixing apparatus that adds water preparation processing prepared therefrom seed.The concrete grammar that adopts during seed dressing comprises to be introduced seed blender, seed dressing preparation itself or water in advance dilution back is added and mixed until preparation with concrete requirement is evenly distributed on the seed.Randomly, then carry out drying process.
In material protection, material of the present invention can be used for the safeguard industries material and avoids infecting of bad mushroom and/or microorganism and damage.
The implication of industrial materials is interpreted as the Manufactured non-living body material that is used for industry among the present invention.For example, treat by active substance of the present invention protection to avoid microorganism and change or the industrial materials of infringement can be adhesive, sizing material, paper and sheet material, fabric, carpet, leather, timber, coating and plastic products, cooling lubricant (cooling lubricant) and other can be subjected to the material of microbial infection or infringement.Can be by the production equipment parts of the breeding of mushroom and/or microorganism infringement, for example chilled(cooling) water return (CWR), cooling and heating system, air-conditioning and ventilating system are also in the scope of material to be protected.Within the scope of the present invention, industrial materials preferred adhesive, sizing material, paper and sheet material, leather, timber, coating, cooling lubricant and the heat exchanger fluid that can mention, preferred especially timber.
Bond of the present invention can prevent unfavorable effect, for example rots, variable color and decolouring or mouldy (molding).
Processing method of the present invention also can be used for protecting stored article to avoid the invasion and attack of mushroom and microorganism.According to the present invention, term " stored article " is interpreted as referring to the natural materials and the various form processing thereof of plant origin or animal origin, and it is taken from the natural life circulation and needs long-term protection.The stored article (for example plant or its part) of the plant origin of that newly gather or form processing; for example stem, leaf, stem tuber, seed, fruit or grain; can obtain protection, described form processing such as pre-dry, wetting, that pulverize, that grind, form squeezing or that bake and bank up with earth.The definition of stored article also comprises timber, and thick timber form (for example construction timber, electric wire tower and fence) or finished commodities form (for example furniture or the article of being made by timber) all can.The stored article of animal origin has rawhide, leather, fur, hair etc.Bond of the present invention can prevent the generation of unfavorable effect, for example rotten, variable color or mouldy.Preferably, " stored article " is interpreted as referring to the natural materials and the form processing thereof of plant origin, more preferably fruit and form processing thereof, for example the operatic circle, drupe, berry and citrus fruit and form processing thereof.
In another preferred embodiment of the present invention, " stored article " is interpreted as referring to timber.Sterilization bond of the present invention or composition also can be used to resist be easy to be grown on the timber or timber in the mushroom disease.All types of converted products that term " timber " means the timber of all seeds types and is intended to the described timber that is used to make up, for example whole timber, high density timber, laminate and plywood.The method of processing timber of the present invention mainly is, itself and one or more compound of the present invention or composition of the present invention are contacted; This for example comprise directly use, spray, flood, injection or any method that other is fit to.
In the disease of plant that can prevent and treat by the inventive method or crop, that can mention has:
The powdery mildew disease, for example:
Bu Shi Erysiphe (Blumeria) disease, (Blumeria graminis) causes by for example grass family Bu Shi powdery mildew;
Podosphaera (Podosphaera) disease is caused by for example white cross hair list softgel shell (Podosphaera leucotricha);
Single softgel shell belongs to (Sphaerotheca) disease, and (Sphaerotheca fuliginea) causes by for example garden balsam list softgel shell;
Uncinula (Uncinula) disease, (Uncinula necator) causes by for example grape snag shell;
The rust disease, for example
Glue Rust (Gymnosporangium) disease, (Gymnosporangium sabinae) causes by for example brown size rest fungus;
Camel spore rust belongs to (Hemileia) disease, is caused by for example coffee camel spore rest fungus (Hemileiavastatrix);
Layer rest fungus (Phakopsora) disease is caused by for example yam bean layer rest fungus (Phakopsorapachyrhizi) and mountain horseleech layer bacterium (Phakopsora meibomiae);
Handle rest fungus (Puccinia) disease is caused by for example Puccinia recondita (Puccinia recondita) and puccinia triticinia (Puccinia triticina);
Uromyces (Uromyces) disease is caused by for example wart top uromyce (Uromycesappendiculatus);
Oomycete class disease, for example
Dish downy mildew (Bremia) disease is caused by for example lettuce dish downy mildew (Bremia lactucae);
Downy mildew (Peronospora) disease is caused by for example pea downy mildew (Peronospora pisi) and Cruciferae downy mildew (Peronospora brassicae);
Epidemic disease mould (Phytophthora) disease, (Phytophthorainfestans) causes by for example phytophthora infestans;
Axle downy mildew (Plasmopara) disease is given birth to axle downy mildew (Plasmoparaviticola) by for example grape and is caused;
False downy mildew (Pseudoperonospora) disease is caused by for example false downy mildew of careless false downy mildew (Pseudoperonospora humuli) and Cuba (Pseudoperonosporacubensis);
Rotten mould (Pythium) disease, mould (Pythium ultimum) causes by for example ultimate corruption;
Leaf spot, leaf spot blight and leaf blight disease, for example
Alternaria (Alternaria) disease is caused by for example early blight chain lattice spore (Alternariasolani);
Cercospora (Cercospora) disease, You Li such as Chard dish are given birth to tail spore (Cercosporabeticola) and are caused;
Cladosporium (Cladiosporium) disease is caused by for example cucumber branch spore (Cladiosporiumcucumerinum);
Cochliobolus belongs to (Cochliobolus) disease, is caused by for example standing grain cochliobolus (Cochliobolussativus);
(conidial form (conidiaform): interior navel Helminthosporium (Drechslera), Syn: the long spore mould (Helminthosporium) of wriggling);
Colletotrichum (Colletotrichum) disease, (Colletotrichum lindemuthianum) causes by for example bean anthrax bacteria;
The Cycloconium disease is caused by for example Cycloconium oleaginum;
Between the seat shell belong to (Diaporthe) disease, by for example between citrus a seat shell (Diaporthe citri) cause;
Elsinoe (Elsinoe) disease is caused by for example citrus Elsinochrome (Elsinoefawcettii);
Coil long spore and belong to (Gloeosporium) disease, cause by for example pleasing the long spore of colour disk (Gloeosporiumlaeticolor);
Small cluster shell belongs to (Glomerella) disease, causes by for example enclosing small cluster shell (Glomerellacingulata);
Ball seat Pseudomonas (Guignardia) disease is caused by for example grape Guignardia (Guignardiabidwelli);
Leptosphaeria (Leptosphaeria) disease, (Leptosphaeria maculans) causes by for example black shank bacterium;
Ruin shell greatly and belong to (Magnaporthe) disease, (Magnaporthegrisea) causes by for example Pyricularia oryzae;
Mycosphaerella (Mycosphaerella) disease is caused by for example standing grain green-ball chamber bacterium (Mycosphaerella graminicola) and banana secret note leaf spot fungi (Mycosphaerallafijiensis);
The Phaeosphaeria disease is caused by for example wheat glume blight bacterium (Phaeosphaerianodorum);
Nuclear cavity Pseudomonas (Pyrenophora) disease is caused by for example round nuclear cavity bacteria (Pyrenophorateres);
Ramularia (Ramularia) disease is caused by for example Ramularia collo-cygni;
Rhynchosporium spp (Rhynchosporium) disease, (Rhynchosporium secalis) causes by for example rye beak spore;
The pin spore belongs to (Septoria) disease, and (Septoria apii) causes by for example Septoria apii;
Nuclear coral Pseudomonas (Typhula) disease is caused by for example meat spore nuclear coral bacterium (Typhula incarnata);
Venturia (Venturia) disease, (Venturiainaequalis) causes by for example apple black star bacteria;
Root and stem disease evil, for example:
Corticium (Corticium) disease is caused by for example Corticium graminearum;
Fusarium (Fusarium) disease is caused by for example sharp sickle spore (Fusarium oxysporum);
The top softgel shell belongs to (Gaeumannomyces) disease, is caused by for example wheat top softgel shell bacterium (Gaeumannomyces graminis);
Rhizoctonia (Rhizoctonia) disease, (Rhizoctoniasolani) causes by for example Rhizoctonia solani Kuhn;
Oculimacula (Tapesia) disease is caused by for example Oculimacula Tapesiaacuformis;
Thiclaviopsis (Thielaviopsis) disease, (Thielaviopsisbasicola) causes by for example thielaviopsis sp;
The spadix and panicled spike (the comprising corncob) disease that cause by for example following pathogene, for example:
The Alternaria disease, (Alternaria spp.) causes by for example Alternaria;
Aspergillus (Aspergillus) disease, (Aspergillus flavus) causes by for example aspergillus flavus;
Cladosporium (Cladosporium) disease is caused by for example Cladiosporiumcladosporioides;
Claviceps (Claviceps) bacterial classification, for example ergot (Claviceps purpurea);
The Fusarium disease is caused by for example yellow sickle spore (Fusarium culmorum);
Gibberella (Gibberella) disease, (Gibberella zeae) causes by for example Gibberella zeae;
Little setting-out shell belongs to (Monographella) disease, causes by for example avenging rotten little setting-out shell (Monographella nivalis);
The sick disease of the black powder fringe of smut and raw meat, for example:
Axle Ustilago (Sphacelotheca) disease, (Sphacelotheca reiliana) causes by for example Sporisorium reilianum;
Tilletia (Tilletia) disease is caused by for example wheat net fungus tilletia (Tilletiacaries);
Urocystis (Urocystis) disease is caused by for example latent bar smut (Urocystisocculta);
Smut (Ustilago) disease, (Ustilago nuda) causes by for example naked smut;
Fruit rot and mildew, for example:
The aspergillus disease is caused by for example aspergillus flavus;
Botrytis (Botrytis) disease, (Botrytis cinerea) causes by for example Botrytis cinerea;
Penicillium (Penicillium) disease is caused by for example penicillium expansum (Penicilliumexpansum) and Penicillium purpurogenum;
Sclerotinia (Sclerotinia) disease, (Sclerotiniasclerotiorum) causes by for example sclerotinite;
Verticillium (Verticillium) disease is caused by for example Huang Verticillium (Verticilliumalboatrum) that withers;
Kind pass with rotting of passing of soil, go rotten, wilting, rot and damping off disease,
Alternaria (Alternaria-Arten) is given birth to chain lattice spores (Alternariabrassicicola) by for example rape and is caused,
Aphanomyces (Aphanomyces-Arten), mould (Aphanomyces euteiches) causes by for example root-rot silk capsule,
Ascochyta (Ascochyta-Arten) is caused by for example Ascochyta lentis;
Aspergillus (Aspergillus-Arten), (Aspergillus flavus) causes by for example aspergillus flavus,
Cladosporium (Cladosporium-Arten) is caused by for example draft branch spore (Cladosporiumherbarum),
Cochliobolus belongs to (Cochliobolus-Arten), and (Cochliobolus sativus) causes by for example standing grain cochliobolus,
(conidial form (konidienform): interior navel Helminthosporium, Bipolaris Syn: the long spore of wriggling is mould);
Colletotrichum (Colletotrichum-Arten) is caused by for example hair nuclear anthrax-bacilus (Colletotrichum coccodes);
Fusarium (Fusarium-Arten) is caused by for example yellow sickle spore (Fusarium culmorum),
Gibberella (Gibberella-Arten), (Gibberella zeae) causes by for example Gibberella zeae,
Shell ball spore belongs to (Macrophomina-Arten), causes by for example Kidney bean shell ball spore (Macrophomina phaseolina),
Monographella-Arten causes by for example avenging rotten bright shuttle spore genus (Monographellanivalis),
Penicillium (Penicillium-Arten), (Penicilliumexpansum) causes by for example penicillium expansum,
Phoma (Phoma-Arten) is caused by for example black shin stem point mould (Phoma lingam),
Phomopsis (Phomopsis-Arten) is caused by for example soybean Phomopsis (Phomopsissojae),
Phytophthora (Phytophthora Arten), (Phytophthoracactorum) causes by for example Phytophthora cactorum,
Nuclear cavity Pseudomonas (Pyrenophora-Arten) is caused by for example wheat class nuclear cavity bacteria (Pyrenophoragraminea),
Pyricularia Sacc. (Pyricularia-Arten), (Pyricularia oryzae) causes by for example Magnaporthe grisea,
Rotten mould (Pythium-Arten), mould (Pythium ultimum) causes by for example ultimate corruption;
Rhizoctonia (Rhizoctonia-Arten), (Rhizoctoniasolani) causes by for example Rhizoctonia solani Kuhn,
Rhizopus (Rhizopus-Arten), (Rhizopus oryzae) causes by for example Rhizopus oryzae,
Bulbil belongs to (Sclerotium-Arten), and (Sclerotiumrolfsii) causes by for example Sclerotium rolfsii,
Septoria (Septoria-Arten), (Septoria nodorum) causes by for example septoria musiva bacterium,
Nuclear coral Pseudomonas (Typhula-Arten) is caused by for example meat spore nuclear coral bacterium (Typhulaincarnata),
Verticillium Nees (Verticillium) is caused by for example big beautiful Verticillium dahliae (Verticilliumdahliae);
Ulcer, witches' broom and the disease disease of dying ack, for example:
Nectria (Nectria) disease is caused by for example apple shuttle scar germ (Nectria galligena);
The blight disease, for example:
Chain sclerotinia sclerotiorum belongs to (Monilinia) disease, is caused by for example drupe chain sclerotinia sclerotiorum (Monilinialaxa);
Leaf blotch exanthema or leaf-curl disease comprise the distortion of flower and fruit, for example:
Exoascus (Taphrina) disease is caused by for example peach external capsule bacterium (Taphrinadeformans);
The degeneration disease of woody plant, for example:
The Esca disease is caused by for example Phaeomoniella clamydospora and Phaeoacremonium aleophilum and Fomitiporia mediterranea;
The disease of flower and seed, for example:
The Botrytis disease is caused by for example Botrytis cinerea;
The stem tuber disease, for example:
The Rhizoctonia disease is caused by for example Rhizoctonia solani Kuhn;
The long mould disease of spore of wriggling is caused by the long spore (Helminthosporium solani) of wriggling of for example eggplant.
The disease that causes by for example following bacterium organism,
Yellow unit cell (Xanthomanas) bacterial classification, for example pathogenic mutation (Xanthomonas campestris pv.Oryzae) of xanthomonas campestris paddy rice;
False unit cell (Pseudomonas) bacterial classification, for example pathogenic mutation (Pseudomonas syringae pv.Lachrymans) of pseudomonas syringae cucumber;
Ou Wenshi (Erwinia) bacterial classification is for example separated starch Erwinia (Erwiniaamylovora).
Compound of the present invention is preferred for preventing and treating following soybean diseases:
The fungal disease of leaf, stem top, beanpod and seed, for example:
Chain lattice spore leaf spot (Alternaria spec.atrans tenuissima), anthracnose (Colletotrichum gloeosporoides dematium var.truncatum), brown spot (the brown line septoria musiva of soybean (Septoria glycines)), tail spore leaf spot and leaf blight (soybean purple speck bacterium (Cercospora kikuchii)), Choanephora leaf blight (choanephora leafblight) (funnel hairpin mould (Choanephora infundibulifera trispora (Syn.))), imperfect fungus leaf spot (dactuliophora leaf spot) (Dactuliophora glycines), downy mildew (green soy bean reveals bacterium germ (Peronospora manshurica)), interior navel Helminthosporium eqpidemic disease (Drechslera glycini), frog eye (grey speck of soybean bacterium (Cercospora sojina)), flax spot Pseudomonas leaf spot (Leptosphaerulina leafspot) (clover helminthosporium leaf spot bacterium (Leptosphaerulina trifolii)), phyllosticta leaf spot (Phyllostica leaf spot) (soybean grey scab bacterium (Phyllosticta sojaecola)), beanpod and cane blight (Phomopsissojae), powdery mildew (scattered cross hair shell (Microsphaera diffusa)), YRENOCHAETA leaf spot (Pyrenochaeta leaf spot) (Pyrenochaeta glycines), rhizoctonia is eqpidemic disease on the ground, leaf blight and damping off (Rhizoctonia solani Kuhn), rust (yam bean layer rest fungus, mountain horseleech layer bacterium), shot hole (soybean scab circle spore (Sphaceloma glycines)), the mould leaf blight of the handle of crawling (Stemphyliumleaf blight) (the handle spore of crawling mould (Stemphylium botryosum)), target spot (Targetspot) (Corynespora cassicola bacterium (Corynespora cassiicola));
The fungal disease of root and the lower portion of the stem, for example:
Black root rot (Caionectria bacterium (Calonectria crotalariae)), charcoal rot (Kidney bean shell ball spore bacterium (Macrophomina phaseolina)), sickle spore fusarium wilt or wilting, root rot and pod and root neck rot (sharp sickle spore, straight beak sickle spore (Fusarium orthoceras), half-naked sickle spore (Fusarium semitectum), scouring rush's sickle spore (Fusarium equiseti)), the mould root rot of lotus spore (Mycoleptodiscus root rot) (water hyacinth spore mould (Mycoleptodiscusterrestris)), new red shell bacterium disease (Neocosmospora) (invading the new red shell bacterium of arteries and veins (Neocosmopspora vasinfecta)), pod blight and cane blight (soybean stem ulcer bacteria (Diaporthe phaseolorum)), stem canker (soybean north stem canker germ (Diaporthephaseolorum var.caulivora)), epidemic disease mould rotten sick (big male epidemic disease mould (Phytophthoramegasperma)), brown stem rot (brown stem rot bacterium (Phialophora gregata)), rotten mildew (pythium rot) (melon and fruit corruption mould (Pythium aphanidermatum), abnormal female corruption mould (Pythium irregulare), pythium debaryanum (Pythium debaryanum), group's knot rotten mould (Pythium myriotylum), ultimate corruption is mould), the rhizoctonia root rot, stem rot and damping off (Rhizoctonia solani Kuhn), sclerotinite stem rot (Sclerotinia stem decay) (sclerotinite), sclerotinite southern blight (neat bulbil bacterium (Sclerotinia rolfsii)), thielaviopsis sp root rot (thielaviopsis sp).
Active agent combinations/composition of the present invention also can be used for preparation and is applicable to therapeutic ground or prophylactically treats human body or the mycotic composition of animal, and described mycosis is mycosis, skin disease, tinea capitis and candidiasis or the disease that caused by aspergillus, for example aspergillus fumigatus (Aspergillusfumigatus) for example.
Active agent combinations/composition of the present invention has good plant tolerance concurrently and to favourable toxicity of warm blooded animal and good environmental resistance; be applicable to insect, arachnid, worm, nematode and mollusk that protective plant and plant organ, raising are gathered and run in productive rate, the quality of improving harvested material and control animal nuisance, particularly agricultural, gardening, herding, forest, park and leisure facilities, storage product and material protection and the health field.It can be preferably used as plant protection product.They to common sensitivity and resistance species and all or some developmental stage have activity.Above-mentioned insect comprises:
Anoplura (Anoplura, Phthiraptera), for example Damalinia (Damalinia spp.), Haematopinus (Haematopinus spp.), Linognathus (Linognathus spp.), lice belong to (Pediculus spp.), Trichodectes (Trichodectes spp.).
Arachnids (Arachnida), for example, Acarus siro (Acarus siro), oranges and tangerines aceria (Aceria sheldoni), peronium Eriophyes (Aculops spp.), acupuncture Eriophyes (Aculus spp.), Amblyomma (Amblyomma spp.), Argas (Argas spp.), Boophilus (Boophilus spp.), short whisker Acarapis (Brevipalpus spp.), Bryobia praetiosa (Bryobia praetiosa), Chorioptes (Chorioptes spp.), Dermanyssus gallinae (Dermanyssus gallinae), beginning Tetranychus (Eotetranychus spp.), goitre mite on the pears (Epitrimerus pyri), true Tetranychus (Eutetranychus spp.), Eriophyes (Eriophyes spp.), half tarsonemid belongs to (Hemitarsonemus spp.), Hyalomma (Hyalomma spp.), hard tick belongs to (Ixodes spp.), latrodectus mactans (Latrodectusmactans), Metatetranychus spp., the unguiculus mite belongs to (Oligonychus spp.), Ornithodoros (Ornithodoros spp.), Panonychus citri belongs to (Panonychus spp.), the tangerine rust mite (Phyllocoptruta oleivora) that rues, Polyphagotarsonemus latus Banks (Polyphagotarsonemus latus), Psoroptes (Psoroptes spp.), Rh (Rhipicephalus spp.), the root mite belongs to (Rhizoglyphus spp.), itch mite belongs to (Sarcoptes spp.), Middle East gold scorpion (Scorpio maurus), Stenotarsonemus spp., tarsonemid belongs to (Tarsonemus spp.), Tetranychus (Tetranychus spp.), Vasateslycopersici.
Bivalve mollusks (Bivalva) guiding principle, for example, the decorations shellfish belongs to (Dreissena spp.).
Lip foot order (Chilopoda), for example, DIWUGONG belongs to (Geophilus spp.), Scutigeraspp..
Coleoptera (Coleoptera), for example, acanthoscelides obtectus (Acanthoscelides obtectus), the beak rutelian belongs to (Adoretus spp.), willow firefly chrysomelid (Agelastica alni), click beetle belongs to (Agriotes spp.), the potato melolonthid (Amphimallon solstitialis), furniture death watch beetle (Anobium punctatum), longicorn beetle belongs to (Anoplophora spp.), flower resembles genus (Anthonomus spp.), Anthrenus (Anthrenus spp.), Ah gill cockchafer belongs to (Apogonia spp.), Atomaria spp., moth-eaten belong to (the Attagenus spp.) of fur, dislike bar bean weevil (Bruchidius obtectus), bean weevil belongs to (Bruchus spp.), tortoise resembles genus (Ceuthorhynchus spp.), Cleonus mendicus, wide chest Agriotes spp (Conoderus spp.), collar resembles genus (Cosmopolites spp.), the brown New Zealand rib wing melolonthid (Costelytra zealandica), Curculio (Curculio spp.), the latent beak of Yang Gan resembles (Cryptorhynchus lapathi), khapra beetle belongs to (Dermestes spp.), chrysomelid genus (Diabrotica spp.), epilachna genus (Epilachna spp.), tobacco boring worm (Faustinus cubae), globose spider beetle (Gibbium psylloides), black different pawl sugarcane cockchafer (Heteronychus arator), Hylamorpha elegans, North America house longhorn beetle (Hylotrupes bajulus), alfalfa leaf resembles (Hypera postica), Hypothenemusspp., the big brown hock gill cockchafer of sugarcane (Lachnosterna consanguinea), colorado potato beetle (Leptinotarsa decemlineata), rice root weevil (Lissorhoptrus oryzophilus), the tube beak resembles genus (Lixus spp.), moth-eaten belong to (the Lyctus spp.) of powder, pollen beetle (Meligethes aeneus), the west melolonthid in May (Melolontha melolontha), Migdolus spp., China ink day Bos (Monochamus spp.), Naupactusxanthographus, golden spider beetle (Niptus hololeucus), coconut palm moth rhinoceros cockchafer (Oryctesrhinoceros), saw-toothed grain beetle (Oryzaephilus surinamensis), black grape ear image (Otiorrhynchus sulcatus), little blue and white cockchafer (Oxycetonia jucunda), horseradish daikon leaf beetle (Phaedon cochleariae), food phyllobranchia cockchafer belongs to (Phyllophaga spp.), Japan popillia flavosellata fairmaire (Popillia japonica), weevil belongs to (Premnotrypes spp.), rape golden head flea beetle (Psylliodes chrysocephala), Ptinus (Ptinus spp.), dark-coloured ladybug (Rhizobius ventralis), lesser grain borer (Rhizopertha dominica), grain weevil belongs to (Sitophilus spp.), point Rhynchophorus (Sphenophorus spp.), stem resembles genus (Sternechus spp.), Symphyletes spp., yellow mealworm (Tenebrio molitor), Tribolium (Tribolium spp.), the spot khapra beetle belongs to (Trogoderma spp.), seed resembles genus (Tychius spp.), ridge tiger day Bos (Xylotrechus spp.), belong to (Zabrusspp.) apart from ground beetle.
Collembola (Collembola), for example, arms Onychiurus arcticus (Onychiurus armatus).
Dermaptera (Dermaptera), for example, European earwig (Forficula auricularia).
Doubly sufficient order (Diplopoda), for example, Blaniulus guttulatus.
Diptera (Diptera), for example, Aedes (Aedes spp.), Anopheles (Anophelesspp.), garden march fly (Bibio hortulanus), calliphora erythrocephala (Calliphoraerythrocephala), Mediterranean Ceratitis spp (Ceratitis capitata), Carysomyia (Chrysomyia spp.), Callitroga (Cochliomyia spp.), people torsalo (Cordylobiaanthropophaga), Culex (Culex spp.), Cuterebra (Cuterebra spp.), the big trypetid of olive (Dacus oleae), human botfly (Dermatobia hominis), Drosophila (Drosophila spp.), Fannia (Fannia spp.), Gasterophilus (Gastrophilus spp.), Hylemyia (Hylemyia spp.), Hyppobosca spp., Hypoderma (Hypoderma spp.), liriomyza bryoniae belongs to (Liriomyza spp.), Lucilia (Lucilia spp.), Musca (Muscaspp.), Bemisia spp (Nezara spp.), Oestrus (Oestrus spp.), Oscinella frit (Oscinella frit), lamb's-quarters spring fly (Pegomyia hyoscyami), grass Hylemyia (Phorbiaspp.), Genus Stomoxys (Stomoxys spp.), Gadfly (Tabanus spp.), Tannia spp., Europe daddy-longlegs (Tipula paludosa), Wohlfahrtia (Wohlfahrtia app.).
Gastropoda (Gastropoda), for example, Arion spp., Biomphalaria (Biomphalariaspp.), little Bulinus (Bulinus spp.), Agriolimax (Deroceras spp.), native snail belong to (Galba spp.), Lymnaea (Lymnaea spp.), Katayama (Oncomelaniaspp.), amber spiro spp (Succinea spp.).
Worm guiding principle (Helminths), for example, Ancylostoma duodenale (Ancylostomaduodenale), Sri Lanka hook worm (Ancylostoma ceylanicum), ancylostoma braziliense (Acylostoma braziliensis), Ancylostoma (Ancylostoma spp.), seemingly draw ascarid nematode (Ascaris lubricoides), Ascaris (Ascaris spp.), cloth Shandong, Malaysia nematode (Brugia malayi), cloth Shandong, Timor nematode (Brugia timori), Bunostomum (Bunostomum spp.), Chabertia belongs to (Chabertia spp.), branch testis fluke belongs to (Clonorchis spp.), Cooperia (Cooperia spp.), Dicrocoelium (Dicrocoelium spp.), dictyocaulus filaria (Dictyocaulus filaria), fish tapeworm (Diphyllobothrium latum), Guinea worm (Dracunculusmedinensis), Echinococcus granulosus (Echinococcus granulosus), Echinococcus multilocularis (Echinococcus multilocularis), pinworm (Enterobiusvermicularis), Faciola spp., blood Trichinella (Haemonchus spp.), Heterakis (Heterakis spp.), short and smallly nibble shell tapeworm (Hymenolepis nana), Metastrongylus apri belongs to (Hyostrongulus spp.), loa loa (Loa Loa), Nematodirus (Nematodirus spp.), oesophagostomum (Oesophagostomum spp.), Opisthorchis (Opisthorchis spp.), Onchocerca caecutiens (Onchocerca volvulus), this off-line Eimeria (Ostertagia spp.) difficult to understand, Paragonimus (Paragonimus spp.), Schistosomen spp., Fu Shi quasi-colubriformis (Strongyloides fuelleborni), strongyloides intestinalis (Strongyloides stercoralis), excrement Strongylus (Stronyloides spp.), taeniarhynchus saginatus (Taenia saginata), taeniasis suis (Taenia solium), trichina(Trichinella spiralis) (Trichinella spiralis), local hair shape nematode (Trichinella nativa), strain Bu Shi trichina (Trichinella britovi), south trichina (Trichinella nelsoni), Trichinella pseudopsiralis, Trichostrongylus (Trichostrongulus spp.), ascaris trichiurus (Trichuris trichuria), wuchereria bancrofti (Wuchereriabancrofti).
In addition, also can prevent and treat protozoa, for example eimeria (Eimeria).
Heteroptera (Heteroptera), for example, squash bug (Anasa tristis), intend beautiful stinkbug and belong to (Antestiopsis spp.), the soil chinch bug belongs to (Blissus spp.), pretty fleahopper belongs to (Calocorisspp.), Campylomma livida, different back of the body chinch bug belongs to (Cavelerius spp.), Cimex (Cimex spp.), Creontiades dilutus, pepper coried (Dasynus piperis), Dichelops furcatus, the long excellent lace bug (Diconocoris hewetti) of Hou Shi, red cotton bug belongs to (Dysdercus spp.), the America stinkbug belongs to (Euschistus spp.), Eurygasterspp belongs to (Eurygasterspp.), Heliopeltis spp., Horcias nobilellus, Leptocorisa spp belongs to (Leptocorisaspp.), leaf beak coried (Leptoglossus phyllopus), lygus bug belongs to (Lygus spp.), sugarcane is deceived chinch bug (Macropes excavatus), Miridae (Miridae), Bemisia spp, Oebalusspp., Pentomidae, side butt stinkbug (Piesma quadrata), the wall stinkbug belongs to (Piezodorusspp.), cotton pseudo-spot leg fleahopper (Psallus seriatus), Pseudacysta persea, Rhodnius (Rhodnius spp.), Sahlbergella singularis (Sahlbergella singularis), black stinkbug belongs to (Scotinophora spp.), pear crown network pentatomidae (Stephanitis nashi), Tibraca spp., Triatoma (Triatoma spp.).
Homoptera (Homoptera), for example, no net long tube Aphis (Acyrthosipon spp.), Aeneolamia spp., grand arteries and veins Psylla spp (Agonoscena spp.), Aleurodes spp., sugarcane Aleyrodes (Aleurolobus barodensis), Aleurothrixus spp. Mango fruit leafhopper belongs to (Amrasca spp.), Anuraphis cardui, the kidney Aspidiotus belongs to (Aonidiella spp.), Soviet Union bloom aphid (Aphanostigma piri), Aphis (Aphis spp), grape leafhopper (Arboridia apicalis), the roundlet armored scale belongs to (Aspidiella spp.), Aspidiotus belongs to (Aspidiotus spp.), Atanus spp., the eggplant ditch does not have net aphid (Aulacorthum solani), Aleyrodes (Bemisia spp.), Lee's short-tail aphid (Brachycaudus helichrysii), Brachycolus spp., cabbage aphid (Brevicoryne brassicae), little brown back rice plant hopper (Calligypona marginata), a beautiful Huang cicadellid (Carneocephala fulgida), cane powder angle aphid (Ceratovacuna lanigera), Cercopidae (Cercopidae), lecanium belongs to (Ceroplastes spp.), strawberry nail aphid (Chaetosiphon fragaefolii), sugarcane yellow snow armored scale (Chionaspis tegalensis), tea green leafhopper (Chlorita onukii), walnut blackspot aphid (Chromaphis juglandicola), dark brown Aspidiotus (Chrysomphalus ficus), corn leafhopper (Cicadulina mbila), Coccomytilus halli, soft a red-spotted lizard belongs to (Coccusspp.), the tea Fischer conceals knurl aphid (Cryptomyzus ribis), Dalbulus spp., Dialeurodesspp., Diaphorina spp., white back of the body armored scale belongs to (Diaspis spp.), Doralis spp., carry out giant coccid and belong to (Drosicha spp.), west rounded tail Aphis (Dysaphis spp.), the ash mealybug belongs to (Dysmicoccus spp.), Empoasca flavescens (Empoasca spp.), woolly aphid belongs to (Eriosomaspp.), Erythroneura spp., Euscelis bilobatus, coffee ground mealybug (Geococcuscoffeae), phony disease of peach poison leafhopper (Homalodisca coagulata), mealy plum aphid (Hyalopterus arundinis), cottonycushion scale belongs to (Icerya spp.), sheet angle leafhopper belongs to (Idiocerus spp.), flat beak leafhopper belongs to (Idioscopus spp.), small brown rice planthopper (Laodelphaxstriatellus), Lecanium spp., lepidosaphes shimer (Lepidosaphes spp.), radish aphid (Lipaphis erysimi), long tube Aphis (Macrosiphum spp.), Mahanarvafimbriolata, sorghum aphid (Melanaphis sacchari), Metcalfiella spp., wheat does not have net aphid (Metopolophium dirhodum), the black flat wing spot of edge aphid (Monellia costalis), Monelliopsis pecanis, tumor aphid genus (Myzus spp.), lettuce is patched up Macrosiphus spp (Nasonoviaribisnigri), rice leafhopper belongs to (Nephotettix spp.), brown planthopper (Nilaparvatalugens), Oncometopia spp., Orthezia praelonga, red bayberry edge aleyrodid (Parabemisia myricae), Paratrioza spp., the sheet armored scale belongs to (Parlatoria spp.), the goitre woolly aphid belongs to (Pemphigus spp.), corn plant hopper (Peregrinus maidis), continuous mealybug belongs to (Phenacoccus spp.), Yang Ping wing woolly aphid (Phloeomyzus passerinii), phorodon aphid (Phorodon humuli), grape phylloxera belongs to (Phylloxera spp.), brown point of sago cycas and armored scale (Pinnaspis aspidistrae), stern line mealybug belongs to (Planococcus spp.), the former giant coccid of pyriform (Protopulvinaria pyriformis), white mulberry scale (Pseudaulacaspispentagona), mealybug belongs to (Pseudococcus spp.), Psylla spp (Psylla spp.), tiny golden wasp belongs to (Pteromalus spp.), Pyrilla spp., the large bamboo hat with a conical crown and broad brim Aspidiotus belongs to (Quadraspidiotusspp.), Quesada gigas, flat thorn mealybug belongs to (Rastrococcus spp.), Rhopalosiphum (Rhopalosiphum spp.), black bourch belongs to (Saissetia spp.), Scaphoides titanus, greenbug (Schizaphis graminum), sago cycas thorn Aspidiotus (Selenaspidusarticulatus), long clypeus plant hopper belongs to (Sogata spp.), white-backed planthopper (Sogatellafurcifera), Sogatodes spp., Stictocephala festina, Tenalapharamalayensis, Tinocallis caryaefoliae, wide chest froghopper belongs to (Tomaspis spp.), sound Aphis (Toxoptera spp.), greenhouse whitefly (Trialeurodes vaporariorum), individual Psylla spp (Trioza spp.), jassids belongs to (Typhlocyba spp.), the point armored scale belongs to (Unaspisspp.), grape root nodule lice (Viteus vitifolii).
Hymenoptera (Hymenoptera), for example, pine sawfoy belongs to (Diprion spp.), real tenthredinidae (Hoplocampa spp.), the hair ant belongs to (Lasius spp.), MonomoriumMayr (Monomoriumpharaonis), Vespa (Vespa spp.).
Isopoda (Isopoda), for example, pillworm (Armadillidium vulgare), comb beach louse (Oniscus asellus), ball pillworm (Porcellio scaber).
Isoptera (Isoptera), for example, Reticulitermes (Reticulitermes spp.), odontotermes (Odontotermes spp.).
Lepidoptera (Lepidoptera), for example, Sang Jian Autographa spp (Acronicta major), hickie is tired of noctuid (Aedia leucomelas), Agrotis (Agrotis spp.), cotton leaf ripple noctuid (Alabama argillacea), do very Noctua (Anticarsia spp.), Barathrabrassicae, cotton lyonetid (Bucculatrix thurberiella), loose looper (Bupaluspiniarius), the yellow volume of flax moth (Cacoecia podana), Capua reticulana, codling moth (Carpocapsa pomonella), winter geometrid moth (Cheimatobia brumata), straw borer spp (Chilo spp.), spruce bunworm (Choristoneura fumiferana), grape codling moth (Clysia ambiguella) (Clysia ambiguella), Cnaphalocerus spp., earias insulana (Eariasinsulana), Anagasta kuehniella (Ephestia kuehniella), pornography and drug moth (Euproctischrysorrhoea), root eating insect belongs to (Euxoa spp.), the dirty Noctua (Feltia spp.) of cutting, greater wax moth (Galleria mellonella), genus heliothis (Helicoverpa spp.), Heliothis (Heliothis spp.), brownly knit moth (Hofmannophila pseudospretella), tea long paper moth (Homona magnanima), apple ermine moth (Hyponomeuta padella), greedy Noctua (Laphygma spp.), the thin moth of apple (Lithocolletis blancardella), green fruit winter noctuid (Lithophane antennata), the grand in vain root eating insect (Loxagrotis albicosta) of beans, Euproctis (Lymantria spp.), malacosoma neustria (Malacosoma neustria), tomato moth (Mamestra brassicae), rice hair shin noctuid (Mocis repanda), mythimna separata (Mythimna separata), Oria spp., Oulema oryzae (Oulema oryzae), small noctuid (Panolis flammea), Pectinophora gossypiella (Pectinophora gossypiella), tangerine lyonetid (Phyllocnistis citrella), Pieris spp (Pieris spp.), diamond-back moth (Plutellaxylostella), prodenia litura belongs to (Prodenia spp.), Pseudaletia spp., soybean noctuid (Pseudoplusia includens), corn borer (Pyrausta nubilalis), Spodoptera (Spodoptera spp.), Thermesia gemmatalis, bag rain moth (Tineapellionella), curtain rain moth (Tineola bisselliella), the green volume of oak moth (Tortrix viridana), powder Noctua (Trichoplusia spp.).
Orthoptera (Orthoptera), for example, tame Xi (Acheta domesticus), oriental cockroach (Blatta orientalis), blatta germanica (Blattella germanica), Gryllotalpa spp (Gryllotalpa spp.), leucophaea maderae (Leucophaea maderae), migratory locusts belong to (Locusta spp.), black locust belongs to (Melanoplus spp.), American cockroach (Periplanetaamericana), desert locust (Schistocerca gregaria).
Siphonaptera (Siphonaptera), for example, Ceratophyllus (Ceratophyllus spp.), Xanthopsyllacheopis (Xenopsylla cheopis).
Comprehensive order (Symphyla), for example, kahikatea worm (Scutigerella immaculata).
Thrips (Thysanoptera), for example, rice thrips (Baliothrips biformis), Enneothrips flavens, flower thrips belong to (Frankliniella spp.), net Thrips (Heliothrips spp.), greenhouse bar hedge thrips (Hercinothrips femoralis), card Thrips (Kakothrips spp.), grape thrips (Rhipiphorothrips cruentatus), hard Thrips (Scirtothrips spp.), Taeniothrips cardamoni, Thrips (Thripsspp.).
Thysanoptera (Thysanura), for example, silverfish (Lepisma saccharina).
Plant nematode comprises, for example, eel Turbatrix (Anguina spp.), Aphelenchoides (Aphelenchoides spp.), thorn Turbatrix (Belonoaimus spp.), umbrella Aphelenchoides (Bursaphelenchus spp.), fuller's teasel stem eelworm (Ditylenchus dipsaci), ball Heterodera (Globodera spp.), helix Eimeria (Heliocotylenchus spp.), Heterodera (Heterodera spp.), minute hand Turbatrix (Longidorus spp.), Meloidogyne (Meloidogyne spp.), Pratylenchidae belongs to (Pratylenchus spp.), similar similes thorne (Radopholus similis), shallow bid revolves Turbatrix (Rotylenchusspp.), burr Turbatrix (Trichodorus spp.), downgrade Turbatrix (Tylenchorhynchusspp.), pulvinulus sword Turbatrix (Tylenchulus spp.), the nematode (Tylenchulussemipenetrans) of partly puncturing, Xiphinema (Xiphinema spp.).
Active agent combinations of the present invention is not only to plant nuisance, health nuisance with store the product nuisance and have activity, and in field of veterinary for example hard tick of parazoon (vermin and internal parasite), soft ticks, itch mite, trombiculid, fly (bite and suck), parasitic fly larva, lice, hair lice, poultry louse and flea also had activity.Described parasite comprises:
Anoplura (Anoplurida), for example Haematopinus, Linognathus, lice genus, Phtirus spp., pipe lice belong to (Solenopotes spp.)
Mallophaga (Mallophagida) and Amblycera (Amblycerina) and thin angle suborder (Ischnocerina), for example hair Trichodectes (Trimenopon spp.), Menopon (Menoponspp.), huge Trichodectes (Trinoton spp.), Bovicola (Bovicola spp.), Werneckiella spp., Lepikentron spp., Damalinia, Trichodectes, Felicola (Felicola spp.)
Diptera and Nematocera (Nematocerina) and Brachycera (Brachycerina), for example Aedes, Anopheles, Culex, Simulium (Simulium spp.), Eusimulium (Eusimulium spp.), owl midge (Phlebotomus spp.), Lutzomyia (Lutzomyiaspp.), Bitting midge (Culicoides spp.), Chrysops (Chrysops spp.), knurl Gadfly (Hybomitra spp.), Atylotus (Atylotus spp.), Gadfly, Chrysozona (Haematopota spp.), Philipomyia spp., honeybee Hippobosca (Braula spp.), Musca, Hydrotaea (Hydrotaea spp.), Genus Stomoxys, Haematobia (Haematobiaspp.), fly does not belong to (Morellia spp.), Fannia, Glossina (Glossina spp.), Calliphora (Calliphora spp.), Lucilia, Carysomyia, Wohlfahrtia, Sarcophaga (Sarcophagaspp.), Oestrus, Hypoderma, Gasterophilus (Gasterophilus spp.), Hippobosca (Hippobosca spp.), Lipoptena (Lipoptena spp.) and Melophagus (Melophagusspp.)
Siphonaptera (Siphonapterida), for example flea belongs to (Pulex spp.), Ctenocephalus (Ctenocephalides spp.), objective flea belongs to (Xenopsylla spp.) and Ceratophyllus
Heteroptera (Heteropterida), for example Cimex, Triatoma, Rhodnius and Triatoma (Panstrongylus spp.)
Blattaria (Blattarida), for example oriental cockroach, American cockroach, blatta germanica and Supella (Supella spp.)
Acari (Acar, Acarina) and back valve order (Metastigmate) and Mesostigmata (Mesostigmata), for example Argas, Ornithodoros (Ornithodorus spp.), residual beak tick belongs to (Otobius spp.), hard tick belongs to, Amblyomma, Boophilus, Dermacentor (Dermacentor spp.), Haemophysalis spp., Hyalomma, Rh, Dermanyssus (Dermanyssus spp.), sting sharp mite and belong to (Raillietia spp.), Pneumonyssus (Pneumonyssus spp.), chest thorn mite belongs to (Sternostoma spp.) and Vespacarus (Varroa spp.)
Axle Acarina (Actinedida) (preceding valve suborder (Prostigmata)) and flour mite order (Acaridida) (Astigmata (Astigmata)), for example honeybee shield mite belongs to (Acarapisspp.), Cheyletiella (Cheyletiella spp.), Ornithocheyletia (Ornithocheyletia spp.), Myobia (Myobia spp.), Psorergates (Psorergates spp.), Demodex (Demodexspp.), Trombidium (Trombicula spp.), Listrophorus spp., Tyroglyphus (Acarusspp.), Tyrophagus (Tyrophagus spp.), have a liking for wooden mite and belong to (Caloglyphus spp.), mite belongs to (Hypodectes spp.) under the neck, the wing mite belongs to (Pterolichus spp.), Psoroptes, Chorioptes, the ear itch mite belongs to (Otodectes spp.), itch mite belongs to, Notoedres (Notoedres spp.), the lump mite belongs to (Knemidocoptes spp.), Cytodites (Cytodites spp.) and Laminosioptes (Laminosioptes spp.).
Active agent combinations/composition of the present invention also is applicable to the arthropods of the following animal of control invasion and attack: agriculture output domestic animal, for example ox, sheep, goat, horse, pig, donkey, camel, buffalo, rabbit, tame chicken, turkey, duck, goose and honeybee; Other pet animal, for example dog, cat, cage bird and pet fish; And so-called experimental animal, for example hamster, cavy, rat and mouse.By preventing and treating described arthropods, situation dead and that output (meat, milk, hair, skin, egg, honey etc.) reduces is deserved with minimizing, thereby but the active agent combinations of the application of the invention makes animal feeding more economical, easier.
Active agent combinations/composition of the present invention is used for veterinary applications and animal feeding in a known way by following form: by for example tablet, capsule, potus, gavage medicine, granule, paste, pill, the mode of feeding (feed-through) method and suppository carries out administration in the intestines; By for example injection (in intramuscular, subcutaneous, intravenous, the peritonaeum etc.), implant and carry out parenteral; Pass through nasal administration; By for example soaking or take a shower, spraying, sprinkle the form percutaneous drug delivery that waters with drop, cleaning, dusting, and by means of the moldings that comprises reactive compound for example necklace, ear tag, tail tag, limbs ligature (limb band), halter, concentrator marker etc.
When being used for domestic animal, poultry, pet animal etc., the preparation (for example pulvis, emulsion, free flowing composition) that active agent combinations of the present invention can be used as the reactive compound that comprises 1 to 80 weight % amount directly use or dilute 100 to 10000 times after use, or they can chemical Medicatedbath lotion (chemical bath) form use.
In addition, found that active agent combinations of the present invention has strong insecticidal action to the insect of damaging industrial materials.
With example and preferred and do not have any restrictive mode and can propose following insect:
Beetle, for example North America house longhorn beetle, Chlorophorus pilosis, the furniture death watch beetle, report dead death watch beetle (Xestobium rufovillosum), Ptilinus pectinicornis (Ptilinus pecticornis), Dendrobium pertinex, pine death watch beetle (Ernobius mollis), Priobium carpini, Lyctus brunneus Stephens (Lyctus brunneus), Africa powder moth (Lyctus africanus), south powder moth (Lyctus planicollis), quercitron moth (Lyctus linearis), pubescence powder moth (Lyctuspubescens), Trogoxylon aequale, minthea rugicollis (Minthes rugicollis), material bark beetle kind (Xyleborus spec.), Tryptodendron spec., coffee black long moth-eaten (Apatemonachus), Mongolian oak long moth-eaten (Bostrychus capucins), brown different wing long moth-eaten (Heterobostrychus brunneus), long moth-eaten plant (the Sinoxylon spec.) of sour jujube, dinoderus minutus (Dinoderus minutus);
Hymenopteran (Hymenopterons), for example big wood wasp (Sirex juvencus), the big wood wasp of fir (Urocerus gigas), safe wood wasp (Urocerus gigas taignus), the Urocerus augur of strengthening;
Termite, for example European kalotermitid (Kalotermes flavicollis), a fiber crops heap sand termite (Cryptotermes brevis), ash point different termite (Heterotermes indicola), American-European reticulitermes flavipe (Reticulitermes flavipes), Sang Te reticulitermes flavipe (Reticulitermessantonensis), southern Europe reticulate pattern termite (Reticulitermes lucifugus), Darwin Australia termite (Mastotermes darwiniensis), the ancient termite (Zootermopsisnevadensis) in Nevada, coptotermes formosanus (Coptotermes formosanus);
Moth (Bristletail), for example silverfish.
The implication of industrial materials is interpreted as non-living body (non-living) material among the present invention, for example, is preferably plastics, adhesive, sizing material, paper and sheet material, leather, timber and Wood Processing goods, and coating composition.
If suitable, describedly promptly can contain other insecticide, and if suitablely also can contain one or more bactericide with composition.
About other possible additive, can be with reference to above-mentioned insecticide and bactericide.
Active agent combinations/composition of the present invention can be used for protecting the object that contacts with salt solution or brackish water, particularly hull, sieve, net, building, harbour and signal system equally, in case produce dirt.
In addition, active agent combinations/composition of the present invention can be used as antifouling composition individually or with other reactive compound in combination.
In family expenses, health and storage product protection; active agent combinations/composition of the present invention also is suitable for preventing and treating the animal pest that exists in the confined space; particularly insect, arachnid and acarid, described confined space is residence, factory floor, office, compartment etc. for example.They can be used for these insects of family expenses desinsection product control individually or with other reactive compound and auxiliary agent in combination.They all have activity to the species of sensitivity and resistance and to whole developmental stage.These insects comprise:
Scorpionida (Scorpionidea), for example yellow scorpion (Buthus occitanus) in Mediterranean.
Acarina, for example adobe tick (Argas persicus), argas reflexus (Argasreflexus), tongue mite subspecies (Bryobia ssp.), Dermanyssus gallinae, sweet mite (Glyciphagusdomesticus) is had a liking for by family, tampan tick (Ornithodorus moubat), brown dog tick (Rhipicephalus sanguineus), eutrombicula alfreddugesi (Trombicula alfreddugesi), Neutrombicula autumnalis, the spy has a liking for skin mite (Dermatophagoidespteronissimus), method is had a liking for skin mite (Dermatophagoides forinae).
Araneida (Araneae), for example catching bird spider (Aviculariidae), circle spider (Araneidae).
Opiliones (Opiliones), for example chelifer (Pseudoscorpiones chelifer), the blind spider of the Chang Shin of Pseudoscorpiones cheiridium, (Opiliones phalangium).
Isopoda, for example comb beach louse, ball pillworm.
Doubly sufficient order, for example Blaniulus guttulatus, mountain cricket Eimeria (Polydesmus spp.).
Lip foot order, for example DIWUGONG belongs to.
Silverfish order (Zygentoma), for example the comb silverfish belongs to (Ctenolepisma spp.), silverfish, robber fireworm (Lepismodes inquilinus).
Blattaria, for example oriental cockroach, blatta germanica, Asia Lian (Blattella asahinai), leucophaea maderae, angle abdomen Lian belong to (Panchlora spp.), wooden Lian belongs to (Parcoblatta spp.), Australian cockroach (Periplaneta australasiae), American cockroach, big brown big Lian (Periplanetabrunnea), smoke Perilpaneta americana (Periplaneta fuliginosa), brown belt blattaria (Supellalongipalpa).
Jump order (Saltatoria), for example tame Xi.
Dermaptera, for example European earwig.
Isoptera, for example kalotermitid belongs to (Kalotermes spp.), Reticulitermes.
Corrodentia (Psocoptera), for example Lepinatus spp., powder corrodent belong to (Liposcelisspp.).
Coleoptera (Coleoptera), for example Anthrenus, the moth-eaten genus of fur, khapra beetle genus, a long ostomatid (Latheticus oryzae), latent instep cuckoo Eimeria (Necrobia spp.), Ptinus, lesser grain borer, grain weevil (Sitophilus granarius), rice weevil (Sitophilus oryzae), corn weevil (Sitophilus zeamais), Stegobium paniceum (Stegobium paniceum).
Diptera, for example Aedes aegypti (Aedes aegypti), aedes albopictus (Aedesalbopictus), aedes taeniorhynchus (Aedes taeniorhynchus), Anopheles, calliphora erythrocephala, great number fiber crops horsefly (Chrysozona pluvialis), five band Culex pipiens pallens (Culexquinquefasciatus), northern house (Culex pipiens), ring beak culex (Culextarsalis), Drosophila (Drosophila spp.), Fannia canicularis (Fannia canicularis), housefly (Musca domestica), owl midge, flesh fly (Sarcophaga carnaria), Simulium, tatukira (Stomoxys calcitrans), the Europe daddy-longlegs.
Lepidoptera, for example lesser wax-moth (Achroia grisella), greater wax moth, Indian meal moth (Plodiainterpunctella), stopper rain moth (Tinea cloacella), bag rain moth, curtain rain moth.
Siphonaptera, for example ctenocephalides canis (Ctenocephalides canis), ctenocephalides felis (Ctenocephalides felis), Pulex irritans (Pulex irritans), chigo (Tungapenetrans), Xanthopsyllacheopis.
Hymenoptera for example blazons hunchbacked ant (Camponotus herculeanus), black smelly ant (Lasius fuliginosus), black ant (Lasius niger), Lasius umbratus, MonomoriumMayr, Paravespula spp., Pavement Ant (Tetramorium caespitum).
Anoplura (Anoplura), for example head louse (Pediculus humanus capitis), body louse (Pediculus humanus corporis), Pemphigus spp., Phylloeravastatrix, crab louse (Phthirus pubis).
Heteroptera, for example cimex hemipterus (Cimex hemipterus), bed bug (Cimexlectularius), phodnius prolixus (Rhodinus prolixus), invasion triatomid (Triatomainfestans).
In the family expenses field of pesticides, they can use individually or with other suitable reactive compound in combination, and described suitable reactive compound is the reactive compound of phosphoric acid ester, carbamates, pyrethroid, anabasine, growth regulator or other known insecticides kind for example.
They can be following form use: aerosol, do not have the spray product of pressure for example pump spraying and atomizer spray, atomization system, Alevaire, foaming agent, gel automatically, have by the evaporant of the evaporant product of the evaporating plate of cellulose or polymer, liquid evaporant, gel and film evaporant, impeller driven, unpowered or passive vapo(u)rization system, catch moth paper, catch the moth bag and catch moth glue, as granule or powder agent, be used for shedding in bait or the Poison bait station (bait station).
In addition, bond of the present invention and composition also can be used for reducing plant and the vegetable material of gathering in the content of mycotoxin, thereby and reduce content by mycotoxin in its food made and the animal feed.
Can be especially but nonexcludability ground proposes following mycotoxin:
Deoxynivalenol (Deoxynivalenole, DON), nivalenol (Nivalenole), 15-Ac-DON, 3-Ac-DON, T2-and HT2-toxin, the plain toxin (Fumonisines) of rotten horse, zearalenone (Zearalenone), moniliformin (Moniliformine), fusarine (Fusarine), anguidin (Diaceotoxyscirpenole, DAS), white muscardine fungi toxin (Beauvericine), enniatine (Enniatine), Fusaroproliferine, Fusarenole, ochracin (Ochratoxines), patulin (Patuline), ergot alkaloid (Ergotalkaloides) and aflatoxin (Aflatoxines), they cause by for example following fungal disease: Fusarium, for example orange sickle spore (Fusarium acuminatum), oat sickle spore (F.avenaceum), F.crookwellense, yellow sickle spore (F.culmorum), F.graminearum schw (F.graminearum) (Gibberella zeae (Gibberella zeae)), scouring rush's sickle spore (F.equiseti), F.fujikoroi, banana sickle-like bacteria (F.musarum), point sickle spore (F.oxysporum), proliferation sickle-like bacteria (F.proliferatum), annual bluegrass sickle spore (F.poae), F.pseudograminearum, elder sickle spore (F.sambucinum) Lu grass sickle spore (F.scirpi), half-naked sickle spore (F.semitectum), eggplant sickle spore (F.solani), intend branch spore sickle spore (F.sporotricholides), F.langsethiae, glue fusarium oxysporum (F.subglutinans), fusarium tricinctum (F.tricinctum), fusarium moniliforme (F.verticillioides) and other fungal disease; And aspergillus kind, Penicillium kind, purple ergot (Claviceps purpurea), Stachybotrys (Stachybotrys spec.) are planted etc.
The extraordinary bactericidal effect of bond of the present invention or composition is shown in following examples.Though single reactive compound only shows weak sterilization drug effect, the drug effect that bond of the present invention or compositions table reveal greater than each compound drug effect simply add and.
If the sterilization drug effect of bond of the present invention or composition is greater than the expection drug effect according to the bond of two or three reactive compound of S.R.Colby, the synergistic effect that then has bactericide, described S.R.Colby expection drug effect (" Calculation of the synergistic andantagonistic responses of herbicide combinations ", Weeds 1967,15,20-22) calculate by as follows:
If
X is the actual measurement drug effect of the compound (A) of given dose (m ppm),
Y is the actual measurement drug effect of the compound (B) of given dose (n ppm),
Z is the actual measurement drug effect of the compound (C) of given dose (r ppm),
E1 is the compound (A) and compound (B) actual measurement together of m and n ppm given dose
Drug effect,
E2 is compound (A), compound (B) and compound (C) the actual measurement drug effect together of m, n and r ppm given dose,
The Colby formula of binary mixture can as followsly define:
The Colby formula of ternary compound can as followsly define:
Drug effect is in %.0% drug effect means the drug effect corresponding to control group, and 100% drug effect means and do not observe disease.
Under the situation of bactericidal effect greater than the expection drug effect of calculating with the Colby formula that actual observation is arrived, bond of the present invention or composition have superadditivity, promptly can be observed synergistic effect.