CN101744148B - Alfalfa plant bug adult forage - Google Patents
Alfalfa plant bug adult forage Download PDFInfo
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- CN101744148B CN101744148B CN2010100344364A CN201010034436A CN101744148B CN 101744148 B CN101744148 B CN 101744148B CN 2010100344364 A CN2010100344364 A CN 2010100344364A CN 201010034436 A CN201010034436 A CN 201010034436A CN 101744148 B CN101744148 B CN 101744148B
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Abstract
The present invention discloses an alfalfa plant bug adult forage. The alfalfa plant bug adult forage comprises the following ingredients: water, wheat bran flour, bean flour, sucrose, choline chloride, ascorbic acid, sorbic acid, cholesterol and yeast. The forage can also comprise egg and honey water. Alfalfa plant bug adults of the same ages and physiological conditions can be obtained with the alfalfa plant bug adult forage for standard test application. A large quantity of alfalfa plant bug adults can be fed with the method. The survival rate of the alfalfa plant bug adults is largely improved, and the hatching rate is relatively stable. The feeding technique is easily carried out at a low price, and is not limited by seasons. The forage is suitable for popularization and application.
Description
Technical field
The invention belongs to experimental animal breeding technology field, relate to a kind of alfalfa plant bug adult forage.
Background technology
The artificial rearing insect is with a long history.Before more than 4,000 years, our ancestors have just learned the raising silkworm.Modern age, insect raising work started from the initial stage in this century, and Bogdanow had raised calliphora vomitoria with man-made feeds in 1908.To the mid-1970s, the insect of raising with man-made feeds has kind more than 700, to the mid-80, has developed into kind more than 1300, relates to 13 orders of Insecta.Singh etc. had once collected the feed formula and the method for breeding (Singh, 1985) of 100 various insects.Xin's six grades that are situated between have also carried out summing up (Xin is situated between six, 1986) to the man-made feeds of various insects, spider and mite class.Tens of kinds of phytophagous insects are used for the research of sex pheromone and hormone by mass rearing; The mass rearing of predatism such as Chrysopa, ladybug, parasitic wasp and parasitic enemy insect has also been obtained breakthrough.
Because deepening continuously and the development of control of insect new technology of entomology research in recent years, the research of subjects such as pesticide screening test, control of insect and insect toxicity, physiology, ecology is to the rush of demand of test with worm, the standardized insect of physiological standard that needs are a large amount of.It is neat that man-made feeds not only can make insect grow, and physiology is consistent, and be the main path that solves seasonal feed shortage under many circumstances, because most of natural foodstuffs are not capable of using, only reliable man-made feeds just can make research continue like winter.
Just carried out the research of tarnished plant bug man-made feeds abroad in the eighties in last century; Domestic Cai Xiaoming etc. have carried out the Primary Study of black striped plant bug man-made feeds; The prescription of its man-made feeds is that the man-made feeds formulated according to Lygus hesperus Knight forms (Debolt; 1985), key component comprises egg, water, sucrose, brewer's yeast, hydromel, analysis for soybean powder, lecithin, vitamin, Choline Chloride etc.
Alfalfa plant bug is that serious a kind of sucking pest takes place in recent years, and main at present employing chemical prevention has significant negative effect to the ecosystem.For strengthening the research of alfalfa plant bug, rational Prevention Technique is proposed, the lot of test population must be arranged; Because of adopting natural feed to raise, not only feeding cost is too high, and incubation rate, survival rate are low; The needs of incompatibility mass rearing; And be in space state in alfalfa plant bug man-made feeds research field both at home and abroad, thereby it is very necessary to develop a kind of suitable alfalfa plant bug man-made feeds always.
Summary of the invention
The purpose of this invention is to provide a kind of alfalfa plant bug adult forage.
Alfalfa plant bug adult forage provided by the invention comprises following component: water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast.
Above-mentioned alfalfa plant bug adult forage can only be made up of said components.Also can comprise egg and hydromel in this feed, certainly, this feed also can only be made up of water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast.The ratio of weight and number of said water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055; Specifically can be 85-115: 9-11: 14-16: 9-11: 0.09-0.11: 0.04-0.06: 0.09-0.11: 0.09-0.11: 0.03-0.06 or 90-110: 9.5-10.5: 14.5-15.5: 9.5-10.5: 0.095-0.105: 0.045-0.055: 0.095-0.105: 0.095-0.105: 0.035-0.055, preferred 100: 10: 15: 10: 0.1: 0.05: 0.1: 0.1: 0.04.The ratio of weight and number of said water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 8.5-11.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055; Specifically can be 85-115: 9-11: 14-16: 9-11: 9-11: 9-11: 0.09-0.11: 0.04-0.06: 0.09-0.11: 0.09-0.11: 0.03-0.06 or 90-110: 9.5-10.5: 14.5-15.5: 9.5-10.5: 9.5-10.5: 9.5-10.5: 0.095-0.105: 0.045-0.055: 0.095-0.105: 0.095-0.105: 0.035-0.055, preferred 100: 10: 15: 10: 10: 10: 0.1: 0.05: 0.1: 0.1: 0.04; The mass percentage concentration of said hydromel is 20-70%, specifically can be 25-65%, 30-60%, 45-50% or 40-60%, preferred 40-50%.Various wheat bran powder, bean powder and the dusty yeasts that satisfy the different meshes of alfalfa plant bug nutritional need all are applicable to this feed.
The method of the above-mentioned alfalfa plant bug adult forage of preparation provided by the invention comprises the steps: water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast mixing are obtained said alfalfa plant bug adult forage;
Perhaps, with water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast mixing, obtain said alfalfa plant bug adult forage.
In this method; The ratio of weight and number of said water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055; Specifically can be 85-115: 9-11: 14-16: 9-11: 0.09-0.11: 0.04-0.06: 0.09-0.11: 0.09-0.11: 0.03-0.06 or 90-110: 9.5-10.5: 14.5-15.5: 9.5-10.5: 0.095-0.105: 0.045-0.055: 0.095-0.105: 0.095-0.105: 0.035-0.055, preferred 100: 10: 15: 10: 0.1: 0.05: 0.1: 0.1: 0.04; The ratio of weight and number of said water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 8.5-11.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055; Specifically can be 85-115: 9-11: 14-16: 9-11: 9-11: 9-11: 0.09-0.11: 0.04-0.06: 0.09-0.11: 0.09-0.11: 0.03-0.06 or 90-110: 9.5-10.5: 14.5-15.5: 9.5-10.5: 9.5-10.5: 9.5-10.5: 0.095-0.105: 0.045-0.055: 0.095-0.105: 0.095-0.105: 0.035-0.055, preferred 100: 10: 15: 10: 10: 10: 0.1: 0.05: 0.1: 0.1: 0.04; The mass percentage concentration of said hydromel is 20-70%, specifically can be 25-65%, 30-60%, 45-50% or 40-60%, preferred 40-50%.
The present invention also provides a kind of method of raising alfalfa plant bug adult, is to place insect box to raise with above-mentioned alfalfa plant bug adult forage provided by the invention alfalfa plant bug adult; Said insect box comprise can mutual airtight cooperation box body and lid, said lid middle part hollows out, and is coated with hospital gauze between said box body and the lid.
In this method, the order number of said hospital gauze is 30 orders; In the said raising step, temperature is 26 ℃ ± 3 ℃, and relative air humidity is 60% ± 10, and in per 24 hours, light application time is 14 ± 2 hours, and be 10 ± 2 hours interlunation.
Alfalfa plant bug adult forage provided by the invention can obtain the worm code test consistent with physiological situation in age and use alfalfa plant bug adult; It is a kind of method for breeding of producing alfalfa plant bug adult in batches; Improved the survival rate of alfalfa plant bug adult greatly; It is stable that ovum is incubated rate; Breeding technology is simple, and cost is low, and no season limit is suitable for applying.
Description of drawings
Fig. 1 is that the feed of different component shown in the table 1 is raised alfalfa plant bug ovum hatching amount (unit is head).
Fig. 2 is the average egg laying amount (unit is grain) that the feed of different component shown in the table 1 is raised alfalfa plant bug adult.
Fig. 3 is the influence of the feed of different component shown in the table 1 to the alfalfa plant bug adult death rate.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described further, but the present invention is not limited to following examples.
Test is raised according to following method with worm: with fresh kidney bean is that feed is raised alfalfa plant bug adult, used insect box comprise can mutual airtight cooperation box body and lid, hollow out in the middle part of the lid, be coated with 30 purpose hospital gauzes between box body and the lid; This insect box can be the plastics crisper of 2L.Put some banded paper in the insect box to increase activity space.During raising, environment temperature is 26 ℃ ± 3 ℃, and relative air humidity is 60% ± 10, and in per 24 hours, light application time is 14 ± 2 hours, and be 10 ± 2 hours interlunation.When reality is raised alfalfa plant bug adult, also can raise according to the method described above.
Get test that raising according to the method described above obtains with individual uniform adult in the worm, in the 150ml plastic cup, raise 5 every glass; Each is handled 5 times and repeats; Wherein, this plastic cup seals with 30 purpose nylon yarn, and at the bottom of cup, puts into four layers of moistening filter paper and collect ovum; Pack in the Fruit yelly case according to getting different component feed and natural feed shown in the table 1, seal, tip upside down on the said 150ml plastic cup with the parafilm film.During raising, temperature is controlled at about 26 ℃ ± 3 ℃, illumination 14 ± 2 hours, dark 10 ± 2 hours, relative humidity about 60% ± 10; Changed a feed in two days, filter paper was changed once in per two days, and kept moistening, write down dead borer population every day, and per two days record layings begin to write down every day ovum hatching amount after one week.In the table 1, the feed that is numbered C is alfalfa plant bug adult forage provided by the invention (wherein, the mass percentage concentration of hydromel is 50%), and the feed that is numbered A, B and D is the feed as other components that contrast, and natural feed is fresh kidney bean; Each component of numbering A-D is parts by weight.
Table 1, feed ingredient table
| Numbering | The wheat bran powder | Bean powder | Egg | Hydromel | Sucrose | Choline Chloride | Ascorbic acid | Sorbic acid | Cholesterol | Dusty yeast | Water |
| A | 5 | 15 | 10 | 0.1 | 0.05 | 0.1 | 0.1 | 0.04 | 100 | ||
| |
10 | 15 | 10 | 10 | 0.1 | 0.05 | 0.1 | 0.1 | 0.04 | 100 | |
| |
10 | 15 | 10 | 10 | 10 | 0.1 | 0.05 | 0.1 | 0.1 | 0.04 | 100 |
| |
5 | 25 | 5 | 10 | 0.1 | 0.05 | 0.1 | 0.1 | 0.04 | 100 |
Fig. 1 is that the feed of different component shown in the table 1 is raised alfalfa plant bug ovum hatching amount, i.e. ovum hatching is the number of alfalfa plant bug nymph.Fig. 2 is the average egg laying amount that the feed of different component shown in the table 1 is raised alfalfa plant bug adult, a number of promptly on average laying eggs.Fig. 3 is the influence of the feed of different component shown in the table 1 to the alfalfa plant bug cumulative mortality.Among above-mentioned Fig. 1-Fig. 3, abscissa is the raising fate.Can know that by figure the death rate that is numbered the alfalfa plant bug that the alfalfa plant bug feed of A raises in the table 1 is low than other processing, is close with natural feed; And egg laying amount and the ovum hatching amount of the alfalfa plant bug that the feed that is numbered C is raised are all higher; Though a little less than contrast natural feed (among Fig. 1-Fig. 3 shown in the curve C K), the adult survival rate will be higher than natural raising, and cost is low more than 1/3; Be fit to mass rearing, do not have season limit.Alfalfa plant bug feed provided by the invention can steady and continuous be raised alfalfa plant bug three generations's man-made feeds.
Claims (12)
1. an alfalfa plant bug adult forage comprises following component: water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast;
In the said alfalfa plant bug adult forage, the ratio of weight and number of water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055.
2. alfalfa plant bug adult forage according to claim 1 is characterized in that: said alfalfa plant bug adult forage is made up of water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast.
3. alfalfa plant bug adult forage according to claim 1 and 2; It is characterized in that: in the said alfalfa plant bug adult forage, the ratio of weight and number of water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 100: 10: 15: 10: 0.1: 0.05: 0.1: 0.1: 0.04.
4. alfalfa plant bug adult forage according to claim 1 is characterized in that: the component of said alfalfa plant bug adult forage also comprises egg and hydromel;
In the said alfalfa plant bug adult forage, the ratio of weight and number of water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 8.5-11.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055;
The mass percentage concentration of said hydromel is 20-70%.
5. alfalfa plant bug adult forage according to claim 4 is characterized in that: said alfalfa plant bug adult forage is made up of water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast.
6. according to claim 4 or 5 described alfalfa plant bug adult forages; It is characterized in that: in the said alfalfa plant bug adult forage, the ratio of weight and number of water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 100: 10: 15: 10: 10: 10: 0.1: 0.05: 0.1: 0.1: 0.04; The mass percentage concentration of said hydromel is 40-50%.
7. method for preparing arbitrary said alfalfa plant bug adult forage among the claim 1-3; Comprise the steps: water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast mixing, obtain the arbitrary said alfalfa plant bug adult forage of claim 1-3;
The ratio of weight and number of said water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055.
8. method according to claim 7 is characterized in that: the ratio of weight and number of said water, wheat bran powder, bean powder, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 100: 10: 15: 10: 0.1: 0.05: 0.1: 0.1: 0.04.
9. method for preparing arbitrary said alfalfa plant bug adult forage among the claim 4-6; Comprise the steps: water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast mixing, obtain the arbitrary said alfalfa plant bug adult forage of claim 4-6;
The ratio of weight and number of said water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 80-120: 8.5-11.5: 13.5-16.5: 8.5-11.5: 8.5-11.5: 8.5-11.5: 0.085-0.115: 0.035-0.065: 0.085-0.115: 0.085-0.115: 0.025-0.055; The mass percentage concentration of said hydromel is 20-70%.
10. method according to claim 9 is characterized in that: the ratio of weight and number of said water, wheat bran powder, bean powder, egg, hydromel, sucrose, Choline Chloride, ascorbic acid, sorbic acid, cholesterol and dusty yeast is 100: 10: 15: 10: 10: 10: 0.1: 0.05: 0.1: 0.1: 0.04; The mass percentage concentration of said hydromel is 40-50%.
11. a method of raising alfalfa plant bug adult is to place insect box to raise with the arbitrary described alfalfa plant bug adult forage of claim 1-6 alfalfa plant bug adult; Said insect box comprise can mutual airtight cooperation box body and lid, said lid middle part hollows out, and is coated with hospital gauze between said box body and the lid.
12. method according to claim 11 is characterized in that: said insect box is a crisper, and the order number of said hospital gauze is 30 orders; In the said raising step, temperature is 26 ℃ ± 3 ℃, and relative air humidity is 60% ± 10, and in per 24 hours, light application time is 14 ± 2 hours, and be 10 ± 2 hours interlunation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250894A (en) * | 2013-02-04 | 2013-08-21 | 吉林省林业科学研究院 | Predatory nature enemy arma chinensis artificial feed preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102771670B (en) * | 2012-08-03 | 2014-03-26 | 中国农业科学院植物保护研究所 | Artificial diet for predative natural enemy insect-true bugs |
| CN105380017A (en) * | 2015-11-17 | 2016-03-09 | 云南农业大学 | Agar-free Drosophila suzukii Matsumura semi-artificial feed |
| CN108402343B (en) * | 2018-04-09 | 2022-03-22 | 华南农业大学 | Artificial feed formula for harris baccarum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
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| CN101507472A (en) * | 2009-03-19 | 2009-08-19 | 中国农业科学院植物保护研究所 | Artificial feedstuff of striped rice borer, preparation method thereof and breeding method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250894A (en) * | 2013-02-04 | 2013-08-21 | 吉林省林业科学研究院 | Predatory nature enemy arma chinensis artificial feed preparation method |
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