CN101743965B - Compound preparation for controlling rubber tree trunk phloem necrosis and preparation method thereof - Google Patents
Compound preparation for controlling rubber tree trunk phloem necrosis and preparation method thereof Download PDFInfo
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- CN101743965B CN101743965B CN 200810183285 CN200810183285A CN101743965B CN 101743965 B CN101743965 B CN 101743965B CN 200810183285 CN200810183285 CN 200810183285 CN 200810183285 A CN200810183285 A CN 200810183285A CN 101743965 B CN101743965 B CN 101743965B
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- dead skin
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Abstract
The invention relates to a compound preparation for controlling rubber tree trunk phloem necrosis and a preparation method thereof. The compound preparation consists of anti-oxidation substance and methylcellulose. The mass ratio of the anti-oxidation substance to the methylcellulose is 0.5-3.0: 1, preferably 1.0-2.5: 1, more preferably 1.5-2.0: 1. The preparation method provided by the invention is simple and feasible. The prepared compound preparation can prevent dead bark and eliminate the occurrence of the dead bark, and has remarkable effect.
Description
Technical field
The present invention relates to a kind of compound formulation of preventing and treating the bamboo grows dead skin disease and preparation method thereof.
Background technology
The same iron and steel of natural rubber, oil, coal are basic industry and the strategic materials that involve the interests of the state and the people together and be called the four big raw materials of industry.As one of the world's four big raw materials of industry; Natural rubber is extensively applied to aspects such as industry, agricultural, national defence, communications and transportation, machine-building, medical and health and daily life because of having very strong elasticity, good insulation performance property, plasticity, wearability, anti-drawing property, water proof gas barrier properties etc., becomes one of indispensable material in national defence and the industry.Lactiferous plant in the world has kind more than 2000, but at present by establishing in large scale and become natural rubber unique commercial source, is Para rubber tree.But the harm that bamboo grows " dead skin disease " already causes for rubber planting is extremely serious, is one of main limiting factor of natural rubber per unit area yield raising.According to latest survey, no matter reclamation area, Hainan, or reclamation area, Yunnan, bamboo grows dead skin rate average out to about 25%.Even more serious is, the dead skin tree is also with 1% speed cumulative year after year in recent years.
Bamboo grows " dead skin disease " is divided into two types of (Jacob JL; Prevot JC; Lacrotte is panel dryness in Hevea brasiliensis.Plantat ions R.1994.Tapping; Recherche, Development, 1 (3): 15-24.): gangrenosum acne " dead skin disease " and non-gangrenosum acne " dead skin disease ".Gangrenosum acne " dead skin disease " be called again " trunk phloem necorsis " (Trunk Phloem Necrosis, TPN).It is characterized in that latex dust and surrounding tissue thereof are old and feeble, brownization of endothelium, but this symptom rapid spread to whole face extend to the non-face on opposite when serious.Just gangrenosum acne " dead skin disease " will occur in the rubber tapping treelet soon, even in the treelet of not rubber tapping, generation arranged also.This dead skin is irreversible, because the bark of regeneration is ill too.Someone thinks, gangrenosum acne " dead skin disease " be pathogen or cyanogen poison cause [Fan Siwei, the cause of disease of the few fine jade .1994. bamboo grows dead skin of poplar and the hypothesis of relevant dead skin. tropical crop is studied, (4): 43-48].The type " dead skin disease " takes place seldom, does not constitute a threat to producing.Non-gangrenosum acne " dead skin disease " is called " face is dry sick " again, and (Tapping PanelDryness TPD), has been acknowledged as a kind of caused by injury (rubber tapping) and ethene stimulation (well stimulation of generally adopting on existing the production), complex physical syndrome at present.The characteristic of non-gangrenosum acne TPD is that histology, the cytology variation of bark is less, but the latex dust metabolic disorder is serious, during sick sending out, and the local or all latex dusts obstructions of bamboo grows secant, the binder removal amount significantly reduces not even binder removal.This TPD is reversible, as long as let bamboo grows rest and reorganize about 1 year, but just rehabilitation of bamboo grows.But this dead skin takes place very general, and production is constituted a serious threat, and what the bamboo grows dead skin of being carried in the industry referred to is exactly this dead skin.Every separated international rubber research and development committee of 2-3 (IRRDB) just holds a dead skin symposium, and that focus on just is non-gangrenosum acne TPD.
Chinese patent ZL93107276.X discloses a kind of manufacturing approach of treating vegetable rubber tree dead skin revivifier, and multiple Chinese herbal medicine is handled, dried, grinding and part liquor, mixes stirring, propagandizes the quantitative package of sieving after (burying) hotly.Brown type, outer brown type, stable type, Phytophthora spp, mycosis, lines parasitosis, virus disease (type of containing restrains diseases such as Ci Shi body) in the medicinal powder treatment intractable bamboo grows dead skin; 1-15 is prolonged to stop cutting the diseases such as bamboo grows dead skin of not producing glue has special curative effect; Coat medicinal powder of the present invention once, just can open after 3-12 month to cut and bring back to life bamboo grows production glue.
Application number is that 97121908.7 one Chinese patent application discloses a kind of preventing and treating chemical for brown spot of rubber tree, and it mainly is made up of tetracyclines (chloramphenicol), methylcellulose, yellow first glue etc.Suitable being coated with on the disease tree face that imposes on brown spot of rubber tree can be prevented and treated brown spot of rubber tree effectively, and can be made rubber trunk glue output raising the output 14.27%, and cost and benefit ratio are 1: 4.53-6.23.
Said method possibly mainly be tackle by cause of disease microbial " trunk phloem necorsis " (Trunk PhloemNecrosis, TPN), as stated; The type " dead skin disease " takes place seldom, does not constitute a threat to producing, therefore; Up to now, also do not see popularization.
" face is dry sick " (Tapping Panel Dryness, TPD), as stated, the type " dead skin " takes place very general, and production is constituted a serious threat, and what the bamboo grows dead skin of being carried in the industry referred to is exactly this dead skin.Up to now, also do not see patent or other reports of any technology of this type of control " dead skin disease ".
In view of this, special proposition the present invention.
Summary of the invention
First purpose of the present invention is to provide a kind of compound formulation of preventing and treating the bamboo grows dead skin disease, and this compound formulation mainly tackles " face is dry sick ", and (Tapping Panel Dryness TPD), can prevent the generation of dead skin, elimination dead skin.
Second purpose of the present invention is to provide a kind of preparation method who prevents and treats the compound formulation of bamboo grows dead skin disease, and this method simple possible is easy to promote.
For realizing first purpose of the present invention, the present invention adopts following technical scheme:
A kind of compound formulation of preventing and treating the bamboo grows dead skin disease, wherein said compound formulation comprises polyphenoils and methylcellulose.
According to the compound formulation of foregoing control bamboo grows dead skin disease, the mass ratio of wherein said polyphenoils and methylcellulose is 0.5～3.0: 1.
According to the compound formulation of foregoing control bamboo grows dead skin disease, the mass ratio of wherein said polyphenoils and methylcellulose is 1.0～2.5: 1.
According to the compound formulation of foregoing control bamboo grows dead skin disease, the mass ratio of wherein said polyphenoils and methylcellulose is 1.5～2.0: 1.
According to the compound formulation of foregoing control bamboo grows dead skin disease, wherein said polyphenoils is one or more in reduced glutathione, ascorbic acid, vitamin E or the beta carotene.
Preferably, described polyphenoils is one or both in reduced glutathione or the ascorbic acid.
For realizing second purpose of the present invention, the present invention adopts following technical scheme:
A kind of preparation method who prevents and treats the compound formulation of bamboo grows dead skin disease, this method are that polyphenoils and methylcellulose is soluble in water.
According to the foregoing preparation method who prevents and treats the compound formulation of bamboo grows dead skin disease, wherein said method is earlier that described polyphenoils is soluble in water, adds described methylcellulose again.
According to the preparation method of the compound formulation of foregoing control bamboo grows dead skin disease, the mass ratio of wherein said described polyphenoils and methylcellulose is 0.5～3.0: 1, preferred 1.0～2.5: 1, more preferably 1.5～2.0: 1.
According to the foregoing preparation method who prevents and treats the compound formulation of bamboo grows dead skin disease; Wherein said polyphenoils is one or more in reduced glutathione, ascorbic acid, vitamin E or the beta carotene, one or both in preferred reduced glutathione or the ascorbic acid.
Below be detailed description of the present invention:
The origin cause of formation of bamboo grows dead skin disease is very complicated.The cause of disease and pathogenesis around the bamboo grows dead skin disease; People once explored and studied from aspects such as pathology, physiology, heredity, envirment factor, soil and biochemistries respectively; Some hypothesis have been drawn; As bark available moisture fluctuation and latex extremely dilute cause a disease says, pathogenic microorganism cause a disease say, the locality severe trauma reacts and says etc., but about the cause of disease of rubber dead skin disease, is much to seek its most essential reason at present.So far the method for still not having efficacious therapy and control bamboo grows dead skin disease.
The inventor adopts mRNA differential display technique (differential display reverse transcriptPCR; DDRT-PCR) in latex, identify and cloned a gene HbMyb1 relevant (plantMolecular Biology 51:51-58,2003) with the dead skin resistance.
The inventor has cloned the HbMyb1 promotor again; And made up the carrier of HbMyb1 promoters driven reporter gene expression; Set up the external transient expression system of latex, utilize this system at laboratory screening to 4 material standed for that effective HbMyb1 of promotion expresses, and be made into compound formulation.Carry out 30 mu of plantation fields and identify, screen 1 prevention dead skin disease effective compound formulation takes place.Qualification result shows that the dead skin rate that the gum that utilizes this compound formulation to handle is compared photograph has reduced by 80% (the dead skin rate in contrast is 100%) relatively.Should be noted that the inventor has adopted the condition (using 2% ethrel) of ethrel super-strong stimulus to carry out the effect that above-mentioned field experiment is identified the prevention dead skin of this compound formulation in order to shorten experimental period.If under the situation of normal use ethrel (being lower than 1%) and science rubber tapping, utilize this compound formulation possibly eliminate the generation of dead skin.And use cost every mu of every year is 8 yuan, in the scope of on producing, accepting.
The compound formulation of control bamboo grows dead skin disease provided by the present invention comprises polyphenoils and methylcellulose.The inventor screens through above-mentioned technology, finds when polyphenoils and methylcellulose are made into compound formulation, can to treat and prevent and treat the bamboo grows dead skin disease effectively.
Among the present invention, the mass ratio of described polyphenoils and methylcellulose is 0.5～3.0: 1, preferred 1.0～2.5: 1, more preferably 1.5～2.0: 1.
The inventor finds in compound formulation, and when the mass ratio of described polyphenoils and methylcellulose is 0.5～3.0: in the time of 1, it prevents and treats the effective of bamboo grows dead skin disease; When its mass ratio is 1.0～2.5: in the time of 1, its effect is better; When its mass ratio is 1.5～2.0: in the time of 1, effect is better.
Compared with prior art, the present invention has following advantage:
1) compound formulation of control bamboo grows dead skin disease provided by the present invention is effective, and the dead skin rate that the gum that utilizes this compound formulation to handle is compared photograph has reduced by 80% (the dead skin rate in contrast is 100%) relatively.Should be noted that the inventor has adopted the condition (using 2% ethrel) of ethrel super-strong stimulus to carry out the effect that above-mentioned field experiment is identified the prevention dead skin of this compound formulation in order to shorten experimental period.If under the situation of normal use ethrel (being lower than 1%) and science rubber tapping, utilize this compound formulation possibly eliminate the generation of dead skin.And use cost every mu of every year is 8 yuan, in the scope of on producing, accepting.And latex diagnosis physiological parameter is measured latex Phos, mercaptan, sucrose, dry rubber content, latex output and the no significant difference of contrast that proof utilizes this compound formulation to handle;
2) compound formulation of control bamboo grows dead skin disease provided by the present invention is used simply, is convenient to the farmland and uses;
3) preparation method of the compound formulation of control bamboo grows dead skin disease provided by the present invention is simple, is convenient to large-scale production.
Embodiment
Below be embodiment of the present invention, described embodiment is in order to further describe the present invention, rather than restriction the present invention.
Embodiment 1
Take by weighing 3 gram beta carotenes, be dissolved in 100 ml waters, add 6 gram methylcellulose, stir, be settled to 1 liter and promptly get bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 2
Take by weighing 8 gram reduced glutathiones, 4 gram beta carotenes and 24 gram methylcellulose, be dissolved in 300 ml waters, stir, placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 3
Take by weighing 15 gram ascorbic acid and 3 gram reduced glutathiones, be dissolved in 300 ml waters, add 12 gram methylcellulose, stir, placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 4
Take by weighing 20 gram ascorbic acid, 3 gram beta carotenes and 1 gram vitamin E, be dissolved in 500 ml waters, add 12 gram methylcellulose, stir, placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 5
Take by weighing 40 gram beta carotenes and 20 gram vitamin Es, be dissolved in 600 ml waters, add 24 gram methylcellulose, stir, placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 6
Take by weighing 75 gram ascorbic acid, 1.5 gram reduced glutathiones, 3.5 gram beta carotenes and 28 gram vitamin Es; Be dissolved in 900 ml waters, add 36 gram methylcellulose, stir; Placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Embodiment 7
Take by weighing 18 gram vitamin Es and 18 gram methylcellulose, be dissolved in 300 ml waters, stir, placement is spent the night, and is settled to 1 liter and promptly gets bamboo grows dead skin disease compound formulation of the present invention.
Test Example 1
Earlier smear ethrel paste (2g/ strain) at the gum face, wait the ethrel paste to handle then back 2 hours, on the tapped part of rubber tree that dries, smear prepared composite preparation of the present invention (3 milliliters/strain) again with brush.
Test Example 2
According to the statistics that 2,210,000 strains is coated with loblolly tree, control efficiency is 75%-95%, after coating is executed the medicament of the embodiment of the invention 3; The incidence of disease of the sick tree of 1-3 level descends 15%; And the coating contrast tree incidence of disease does not rise 30%, spreads the gumwood of this medicament, and dried glue output generally has a net increase of produces 20-40%; The dried glue 28% of average raising the output, dry rubber content has a net increase of 10-15%.Bamboo grows spreads medicament of the present invention, and average every strain increases income 3.5-5.5 unit, and cost and benefit ratio are 1: 6-8.
After coating was executed the medicament of other embodiment of the present invention, its result was similar with the result of present embodiment.
Claims (9)
1. compound formulation of preventing and treating the bamboo grows dead skin disease; It is characterized in that: described compound formulation comprises polyphenoils and methylcellulose; The mass ratio of described polyphenoils and methylcellulose is 0.5～3.0: 1, and described polyphenoils is one or more in reduced glutathione, ascorbic acid, vitamin E or the beta carotene.
2. compound formulation according to claim 1 is characterized in that: the mass ratio of described polyphenoils and methylcellulose is 1.0～2.5: 1.
3. compound formulation according to claim 3 is characterized in that: the mass ratio of described polyphenoils and methylcellulose is 1.5～2.0: 1.
4. according to any described compound formulation of claim 1-3, it is characterized in that: described polyphenoils is one or both in reduced glutathione or the ascorbic acid.
5. preparation method who prevents and treats the compound formulation of bamboo grows dead skin disease is characterized in that: described method is for soluble in water with polyphenoils and methylcellulose; The mass ratio of described polyphenoils and methylcellulose is 0.5～3.0: 1, and described polyphenoils is one or more in reduced glutathione, ascorbic acid, vitamin E or the beta carotene.
6. the preparation method of compound formulation according to claim 5 is characterized in that: described method adds described methylcellulose again for earlier that described polyphenoils is soluble in water.
7. according to the preparation method of claim 5 or 6 described compound formulations, it is characterized in that: the mass ratio of described polyphenoils and methylcellulose is 1.0～2.5: 1.
8. the preparation method of compound formulation according to claim 7, it is characterized in that: the mass ratio of described polyphenoils and methylcellulose is 1.5～2.0: 1.
9. the preparation method of compound formulation according to claim 5, it is characterized in that: described polyphenoils is one or both in reduced glutathione or the ascorbic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810183285 CN101743965B (en) | 2008-12-12 | 2008-12-12 | Compound preparation for controlling rubber tree trunk phloem necrosis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810183285 CN101743965B (en) | 2008-12-12 | 2008-12-12 | Compound preparation for controlling rubber tree trunk phloem necrosis and preparation method thereof |
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| Publication Number | Publication Date |
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| CN101743965A CN101743965A (en) | 2010-06-23 |
| CN101743965B true CN101743965B (en) | 2012-09-05 |
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| CN 200810183285 Expired - Fee Related CN101743965B (en) | 2008-12-12 | 2008-12-12 | Compound preparation for controlling rubber tree trunk phloem necrosis and preparation method thereof |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889580B (en) * | 2010-07-22 | 2011-12-14 | 海南橡宝生物科技有限公司 | Composition for controlling tapping panel dryness |
| CN103011956B (en) * | 2012-12-11 | 2014-02-12 | 任建国 | Preparation for preventing and treating tapping panel dryness of rubber trees as well as preparation method and application thereof |
| CN104291953B (en) * | 2014-09-24 | 2016-06-15 | 江阴海达橡塑股份有限公司 | A kind of preparation method of the medicament preventing and treating rubber tree dead skin |
| CN104620881A (en) * | 2015-03-10 | 2015-05-20 | 中国热带农业科学院橡胶研究所 | Infusion method for preventing and treating tapping panel dryness and nutrient solution |
| CN106577657A (en) * | 2016-12-06 | 2017-04-26 | 云南农业大学 | Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid |
| CN106748131A (en) * | 2016-12-21 | 2017-05-31 | 广西睿桂涵农业有限公司 | A kind of rubber tree dedicated fertilizer |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391803A (en) * | 2002-07-04 | 2003-01-22 | 中国热带农业科学院橡胶栽培研究所 | Rubber production promoter for rubber tree |
| CN1561721A (en) * | 2004-03-19 | 2005-01-12 | 何向东 | Rubber yield promoter for rubber tree |
-
2008
- 2008-12-12 CN CN 200810183285 patent/CN101743965B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391803A (en) * | 2002-07-04 | 2003-01-22 | 中国热带农业科学院橡胶栽培研究所 | Rubber production promoter for rubber tree |
| CN1561721A (en) * | 2004-03-19 | 2005-01-12 | 何向东 | Rubber yield promoter for rubber tree |
Non-Patent Citations (2)
| Title |
|---|
| 张云霞等.橡胶树死皮病研究进展.《热带农业科学》.2006,(第05期), * |
| 龚标勋.橡胶树死皮病及防治技术.《植保技术与推广》.2000,(第01期), * |
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| CN101743965A (en) | 2010-06-23 |
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