CN101698027B - Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof - Google Patents
Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composite for treating infectious bursal disease and a preparation method thereof. The traditional Chinese medicine composition is prepared from glossy privet fruit, wolfberry fruit, dodder and astragalus root according to certain proportions, and has simple formula, low cost and stable quality. When used for treating the infectious bursal disease, the traditional Chinese medicine composite can effectively treat the infectious bursal disease, the effective rate is 91.7-94.2%, the death rate of infected chicken is reduced to 31.7-37.5%, and the traditional Chinese medicine composite not only has exact curative effect but also has no toxic and side effects and drug residues, improves the cultivation benefit and simultaneously avoids causing body harm to people who eat chicken. The traditional Chinese medicine composite is prepared by the steps of pulverizing the raw materials, leaching and concentrating the leaching solution, has stable product quality, simple technology, easily controlled conditions and no discharge of three wastes in the production process, is environment-friendly and is very suitable for industrialized production.
Description
Technical field
The present invention relates to Chinese medicine composition, particularly a kind of Chinese medicine composition of treating infectious bursal disease and preparation method thereof.
Background technology
Propelling along with modern farming; The intensive culture degree is higher; Aviculture (particularly poultry husbandry) has obtained fast development; Yet eqpidemic disease control the fine or not success or failure that directly have influence on poultry husbandry, infectious bursal disease (IBD) is acute, height contact, the immunosuppressive disease of a kind of main infringement chickling that caused by chicken infectivity bursa of Fabricius virus (IBDV), from late 1970s since China occurs; Be diffused into each provinces and cities of China rapidly, brought very big harm to poultry husbandry.Though existing have multiple vaccine can supply prevention, for various reasons, the situation of immuning failure happens occasionally, so that epidemic situation is continuous; As replenishing of vaccine immunity, the yellow antibody of high-immunity egg has become the important means of control IBD.Yet can there be the potential danger of some anthropochory egg source property eqpidemic diseases inevitably in the extensive use of the yellow antibody preparation of high-immunity egg; And still do not have at present this sick specific treatment medicine.So technical problems such as solution intensification chicken farm control infectious bursal disease are very urgent.
With the Chinese herbal treatment bursal disease is to put into practice and prescription according to modern antibiotic, the antiviral of Basic Theories of Chinese Medicine, Chinese medicine characteristic, Chinese medicine.According to the Chinese medicine characteristic, by Chinese veterinarian's theory analysis, bursal disease belongs to due to the pyretic toxicity invasion and attack.Heat is YANG pathogen, is prone to impairment of YIN liquid, so performance drink desire increases; Because the pathogenic heat attacking blood system in febrile diseases, the absurd row of forcing blood, it is hemorrhage to cause muscle etc. to be located; Because of the pathogenic heat transformation of fire and occurrence of wind syndrome, wind helps the intensity of a fire again, on disturb gods, so sick chicken lethargy, lassitude; The bright kidney yin of pyretic toxicity consumption is so see nephrolith; Pyretic toxicity is prone to consumption Tianjin blood trouble, thereby causes origin exhausted, and is so morbidity is anxious, dead fast.Chinese medicine Radix Isatidis, Folium Isatidis, Flos Lonicerae have effects for removing toxic heat, and not only the inhibitory anti-virus effect is strong, and has a broad antifungal spectrum; Radix Codonopsis, Radix Astragali air making-up and spleen enlivening, the enhance immunity function is of value to the immunosuppressant improvement that bursal disease causes.Radix Isatidis, Folium Isatidis, Flos Lonicerae, Herba Taraxaci heat-clearing and toxic substances removing have stronger antibiosis and antiviral functions, eliminate to cause a disease because of; Radix Scutellariae, Cortex Phellodendri clearing away heat-damp and promoting diuresis, Herba Pogostemonis removing dampness by means of aromatics, the symptom of diarrhea of releasing bursal disease; Herba Plantaginis diuresis antidiarrheal can promote the urate of kidney to get rid of, and eliminates renal swelling, again can antidiarrheal; Radix Glycyrrhizae have QI invigorating with in, the effect of coordinating the actions of various ingredients in a prescription.
Research shows that the mechanism of herbal control infectious bursal disease is divided into following several kinds: 1, regulate immunity through acting on hypothalamus, hypophysis, adrenal cortex axle; 2, through regulating the vegetative nerve regulating immunity; 3, regulate immunity through acting on cyclic nucleotide system; 4, regulate immunity through the adjustment nucleic acid metabolism; 5, regulate immunity through improving T cell quantity and function; 6, regulate immunity etc. through the dual regulation of medicine and prescription itself.But present present situation is though it is a lot of effectively to prevent and treat the Chinese medicine formula of infectious bursal disease, all not get into production line basically, so also fail to bring into play its real effect.Therefore; Filter out that anti-infectious bursal disease effect is strong, cheap, on the basis of the Chinese medicine formula of low toxicity noresidue; Let Chinese medicine formula can drop into standardization, large-scale production and be applied to aquaculture; And then fundamentally replace the medicine for treatment of anti-infectious bursal disease, to the sound development that promotes poultry husbandry and ensure the healthy significant of human body.
Therefore, need a kind of Chinese medicine composition of treating infectious bursal disease, prepare burden simple, with low cost, steady quality, determined curative effect is with rapid.
Summary of the invention
One of the object of the invention provides a kind of Chinese medicine composition of treating infectious bursal disease, and simple, with low cost, the steady quality of preparing burden, determined curative effect are rapidly and have no side effect and drug residue.
The Chinese medicine composition of treatment infectious bursal disease of the present invention, crude drug comprise following composition by weight percentage: 40-50% Fructus Ligustri Lucidi, 20-25% Fructus Lycii, 15-20% Semen Cuscutae and the 18-23% Radix Astragali.
As further improvement, said Chinese medicine composition is a granule.
Fructus Ligustri Lucidi (Ligustrum lucidum Ait.) odorless, sweet in the mouth, little bitterness are gone into liver, kidney channel; Its fruit contains effective ingredient such as oleanolic acid (Oleanolic acid), mannitol, glucose, Palmic acid, stearic acid, oleic acid, linoleic acid; Raise immunity is arranged; The rising peripheral leukocytes strengthens reticuloendothelial system phagocytic activity, strengthens the effect of cellular immunization and humoral immunization;
Fructus Lycii (Fructus Lycii.) property is put down sweet in the mouth, goes into liver, kidney channel; It contains effective ingredient such as carotene, betanin, vitamin A, vitamin B1, vitamin B2, vitamin C and calcium, phosphorus, ferrum, has the leukocyte increasing activity, promotes the pharmacological action that hepatocyte is newborn;
Semen Cuscutae (Cuscuta chinensis Lam.) sweet in the mouth warm in nature is returned the liver,kidney,spleen warp; Effective ingredient such as its resinous glycosides, saccharide, flavone compound can strengthen the Bufo siccus cardiac contractile force that exsomatizes; Reduce anaesthetized dog blood pressure; Suppress bowel movement; Excited isolated uterine; Delay the development of rat galactose-cataract, and certain therapeutical effect is arranged; Mice " yang deficiency " model to due to the hydrocortisone has therapeutical effect, and can strengthen nonspecific resistance etc.;
The Radix Astragali (Radix Astragali) sweet in the mouth warm in nature is returned lung, spleen channel; It contains effective ingredient such as astragalus polysaccharides and astragaloside, and the ability enhancing body's immunological function promotes immune organ to grow, and specific immunity and nonspecific immunity is all had facilitation, and antibacterial action is more widely arranged;
The present invention filters out best prescription on the basis of the Chinese medicine composition prescription medicine efficacy screening of treatment infectious bursal disease, prescription is simple, and drug effect is remarkable, has no side effect and drug residue, and is safe in utilization and environmentally friendly.
As further improvement, the present invention has further proposed the Chinese medicine composition of the treatment infectious bursal disease of granule dosage form, and reason is that the Chinese medicine composition of great majority control infectious bursal disease still rests on traditional prescription, compatibility, pulverizes, sieves and process the rough stage of powder or powder; And powder and powder are prone to deposition and caking after to water, and sick chicken usually can't be taken in the effective dose of medicine thing, causes curative effect of medication to be difficult for performance fully; And no matter water preparation, powder or powder all are unfavorable for the formulation of product standard and the evaluation of drug effect; Thereby make product quality restive, further influence its industrialization and produce and apply, so the present invention combines physiological structure of chicken and the characteristics that chicken hair dyeing happiness is after being ill drunk; The effective ingredient of said prescription is refined into granule; Through the diversion administration, be beneficial to the chicken bulk absorption, curative effect is rapider; Not only effectively treat infectious bursal disease; And making the formulation of product standard and the evaluation of drug effect become possibility, the granule dosage form is beneficial to and stores and transportation simultaneously, is convenient to the promotion and application on a large scale of product when improving safety in utilization.
Two of the object of the invention provides a kind of method for preparing the Chinese medicine composition of said treatment infectious bursal disease, comprises the steps:
(1) said crude drug is pulverized the back and mix with the water of 8-10 times of weight and heat lixiviate, the temperature of controlling the lixiviate feed liquid is 75 ℃～80 ℃ and lixiviate 1 hour under reflux state, then material is filtered and obtains lixiviating solution and medicinal residues;
The medicinal residues that (2) will filter gained (1) said method set by step carry out 2-3 time lixiviate again and obtain the lixiviating solution of each time lixiviate generation respectively;
(3) carry out concentrating under reduced pressure after the lixiviating solution of step (1) and step (2) gained is merged, the temperature of control lixiviating solution is at 70 ℃～80 ℃ in the concentration process, and the 1-2 that the weight of treating lixiviating solution is reduced to crude drug weight stops heating and obtains concentrating lixiviating solution after doubly;
(4) the concentrated lixiviating solution of step (3) gained is lowered the temperature.
Further; In the step (4); Concentrated lixiviating solution temperature adds chitosan solution when reducing to 50 ℃～60 ℃ and stirs; Leave standstill 24h and go deposition after the temperature of waiting to contain the concentrated lixiviating solution of chitosan is reduced to room temperature, the mass concentration of said chitosan solution is 1%, and the weight of chitosan solution is for concentrating 10%～15% of lixiviating solution weight;
Further, also comprise the steps:
A. the filtrating of step (4) gained is carried out concentrating under reduced pressure, the weight of treating said filtrating is concentrated into and adds dextrin and sucrose and mix homogeneously behind the 25-40% of crude drug weight and obtain mixture;
B. step a gained mixture extruding is sieved and dry under 60-65 ℃ condition, obtain the Chinese medicine composition product of granule dosage form at last.
Further, among the step a, the temperature when filtrating concentrates is 70-80 ℃;
Further, the weight ratio of said sucrose and dextrin is 2.5-4: 1.
The method for preparing of the Chinese medicine composition of treatment infectious bursal disease of the present invention; Through reasonable control operations index, ensured that effective medicinal ingredient is not destroyed and can be come out by abundant lixiviate in the crude drug, simple, the easy control of condition of technology simultaneously; Three-waste free discharge in the production process; Environmentally friendly, the Chinese medicine composition of the treatment infectious bursal disease that makes through the inventive method, steady quality, evident in efficacy reliable; The effective percentage of treatment infectious bursal disease is 91.7-94.2%; Reducing disease chicken death rate is 31.7-37.5%, can help the raiser significantly to increase economic efficiency, and method for preparing of the present invention is suitable for industrialization production very much.
As further improvement; Method for preparing of the present invention also adds chitosan in concentrated lixiviating solution temperature-fall period; Chitosan can be removed many unsettled microgranules such as protein, manganese liquid matter, natural gum, saddle matter etc.; Use the lixiviate fluid power after concentrating to keep effective ingredient, safety non-toxic largely.
Other advantages of the present invention, target and characteristic will be set forth in description subsequently to a certain extent; And to a certain extent; Based on being conspicuous to those skilled in the art, perhaps can from practice of the present invention, obtain instruction to investigating of hereinafter.
The specific embodiment
Below will carry out detailed description to the preferred embodiments of the present invention.Should be appreciated that preferred embodiment has been merely explanation the present invention, rather than in order to limit protection scope of the present invention.
Embodiment one:
(1) 4.2kg Fructus Ligustri Lucidi, 2.3kg Fructus Lycii, the 2.0kg Radix Astragali and 1.5kg Semen Cuscutae being pulverized the back packs in the Chinese medicine extraction equipment through the Steam Heating lixiviate with 80kg water; The temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 75-77 ℃;
(2) according to the said method of step (1) medicinal residues that filter gained are carried out 2 lixiviates again, and the lixiviating solution that obtains twice lixiviate respectively and produced;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure through steam in the Chinese medicine extraction equipment; The temperature of lixiviating solution is controlled at 70-72 ℃ in the concentration process, stops when treating the lixiviating solution weight reduction to 10kg concentrating and obtains concentrating lixiviating solution;
(4) temperature of treating the concentrated lixiviating solution of step (3) gained is reduced to the Chinese medicine composition that is the required treatment infectious bursal disease of present embodiment after the room temperature.
The effect of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment gained:
270 healthy chickens of 30 ages in days are divided into three groups, and group I is the normal healthy controls group, and group II is the counteracting toxic substances matched group, and group III is a test group, and group II is divided into 4 groups more separately with the chicken of group III; Group I only feeds with normal feedstuff; Group II uses the normal feedstuff identical with the normal healthy controls group to feed; Not administration behind the counteracting toxic substances; Group III uses the normal feedstuff identical with the normal healthy controls group to feed, and after counteracting toxic substances the infectious bursal disease typical clinical symptom occurred in 48 hours, in test group drinking-water, adds the Chinese medicine composition that makes in the present embodiment, and the Chinese medicine composition that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law; Observe disease chicken symptom when 9:00 and 16:00 every day, and dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and performs itemized record; Carry out dead chicken being weighed, dissecting in the 9th day to test; And record pathological changes situation, after test is accomplished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data is seen table-1.
Table-1
| Index is handled | Sick chicken number | Death toll | Cure number | Significant figure | Mortality rate (%) | Cure rate (%) | Effective percentage (%) |
| Group III | 120 | 8 | 108 | 112 | 6.7 | 90.0 | 93.3 |
| Group II | 120 | 48 | - | - | 40 | -- | -- |
| Group I | 0 | 0 | - | - | 0 | -- | -- |
Notes: death is all classical symptom and dead to occur at duration of test, and necropsy has bursal disease virus apparition person, is judged to infect death;
Effectively after medication, dead chicken be not effectively, and the percentage rate that every group of chicken number of surviving accounts for the test chicken number is an effective percentage;
Recovery from illness is after medication, and it is normal that the mental status, appetite etc. are recovered, and all belongs to recovery from illness, and the percentage rate that recovery from illness chicken number accounts for the test chicken number is a cure rate.
Data explanation from table: the Chinese medicine composition of the treatment infectious bursal disease that present embodiment makes can be treated infectious bursal disease effectively, and effective percentage is 93.3%, compares with the counteracting toxic substances matched group, reduces disease chicken death rate 33.3%.
To test chicken and all slaughter, pathological changes such as damage of the fabricius bursa of anatomic observation chicken and hemorrhage of muscle degree, and represent its pathological change degree with score value, relevant data is seen table 2:
Table-2
| Dissect disease chicken number | Score value (meansigma methods ± SD standard error) | |
| Group III | 120 | 1.062±0.854* |
| Group II | 120 | 2.250±0.683 |
| Group I | 30 | 0 |
Can find out that from last table the pathology score value of the test group chicken of administration significantly is lower than the pathology score value (P＜0.05) of counteracting toxic substances matched group chicken, show that the Chinese medicine composition product of present embodiment can obviously improve the anti-infection ability of chicken to bursa of Fabricius virus.
Need to prove that in table of present embodiment-1 and the table-2, the data of group II are the meansigma methods of its 4 group's experimental datas, the data of group III are the meansigma methods of its 4 group's experimental datas.
Embodiment two:
(1) 4.5kg Fructus Ligustri Lucidi, 2.5kg Fructus Lycii, the 2.3kg Radix Astragali and 1.7kg Semen Cuscutae being pulverized the back packs in the Chinese medicine extraction equipment through the Steam Heating lixiviate with 90kg water; The temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 77-80 ℃;
(2) according to the said method of step (1) medicinal residues that filter gained are carried out 2 lixiviates again, and the lixiviating solution that obtains twice lixiviate respectively and produced;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure through steam in the Chinese medicine extraction equipment; The temperature of lixiviating solution is controlled at 78～80 ℃ in the concentration process, treats that the lixiviating solution weight reduction is to stop behind the 20kg concentrating and obtain concentrating lixiviating solution;
When (4) treating that concentrated lixiviating solution temperature is reduced to 50 ℃; In concentrating lixiviating solution, add mass concentration and be 1% chitosan solution and stir after left standstill 24 hours, filter the deposition of removing in the solution then, obtain filtrating; Wherein, the weight of chitosan solution is for concentrating 10% of lixiviating solution weight.
The method for preparing of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment also comprises the steps:
A. the filtrating of step (4) gained is carried out concentrating under reduced pressure, the weight of treating said filtrating adds an amount of dextrin and sucrose and mix homogeneously when being concentrated to 4kg and obtains mixture, and the weight ratio of said dextrin and sucrose is 2.5: 1.
B. step a gained mixture is pushed 12 mesh sieves and dry under 60 ℃ condition, obtained the Chinese medicine composition product of granule dosage form at last.
Certainly, among the present embodiment step a, it is that the 25-40% of crude drug weight all can realize the object of the invention that filtrate decompression is concentrated into weight, and the weight ratio of dextrin and sucrose is 2.5-4: 1 all can ensure the processing and the molding of granule in the subsequent technique.
The effect of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment gained:
270 healthy chickens of 30 ages in days are divided into three groups at random, and group I is the normal healthy controls group, and group II is the counteracting toxic substances matched group, and group III is a test group, and group II is divided into 4 groups more separately with the chicken of group III; Group I only feeds with normal feedstuff; Group II uses the normal feedstuff identical with the normal healthy controls group to feed; Not administration behind the counteracting toxic substances; Group III uses the normal feedstuff identical with the normal healthy controls group to feed, and after counteracting toxic substances the infectious bursal disease typical clinical symptom occurred in 48 hours, in test group drinking-water, adds the Chinese medicine composition that present embodiment makes, and every day, Chinese medicine composition that every chicken is joined was suitable with 1.0 gram raw material of Chinese medicine doses; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law; Observe disease chicken symptom when 9:00 and 16:00 every day, and dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and performs itemized record; Carry out dead chicken being weighed, dissecting in the 9th day to test; And record pathological changes situation, after test is accomplished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data is seen table-3.
Table-3
| Index is handled | Sick chicken number | Death toll | Cure number | Significant figure | Mortality rate (%) | Cure rate (%) | Effective percentage (%) |
| Group III | 120 | 7 | 110 | 113 | 5.8 | 91.7 | 94.2 |
| Group II | 120 | 52 | - | - | 43.3 | -- | -- |
| Group I | 0 | 0 | - | - | 0 | -- | -- |
Notes: death is all classical symptom and dead to occur at duration of test, and necropsy has bursal disease virus apparition person, is judged to infect death;
Effectively after medication, dead chicken be not effectively, and the percentage rate that every group of chicken number of surviving accounts for the test chicken number is an effective percentage;
Recovery from illness is after medication, and it is normal that the mental status, appetite etc. are recovered, and all belongs to recovery from illness, and the percentage rate that recovery from illness chicken number accounts for the test chicken number is a cure rate.
Data explanation from table-3: the Chinese medicine composition of the treatment infectious bursal disease that present embodiment makes can be treated infectious bursal disease effectively, and effective percentage is 94.2%, compares with the counteracting toxic substances matched group, reduces disease chicken death rate 37.5%.
To test chicken and all slaughter, pathological changes such as damage of the fabricius bursa of anatomic observation chicken and hemorrhage of muscle degree, and represent its pathological change degree with score value, relevant data is seen table-4:
Table-4
| Dissect disease chicken number | Score value (meansigma methods ± standard error) | |
| Group III | 120 | 1.042±0.539* |
| Group II | 120 | 3.013±0.857 |
| Group I | 30 | 0 |
Can find out that from last table the pathology score value of the test group chicken of administration significantly is lower than the pathology score value (P＜0.05) of counteracting toxic substances matched group chicken, show that the Chinese medicine composition product of present embodiment can obviously improve the anti-infection ability of chicken to bursal disease virus.
Need to prove that in table of present embodiment-3 and the table-4, the data of group II are the meansigma methods of its 4 group's experimental datas, the data of group III are the meansigma methods of its 4 group's experimental datas.
Embodiment three:
(1) with packing in the Chinese medicine extraction equipment through the Steam Heating lixiviate with 80kg water behind 4.0kg Fructus Ligustri Lucidi, 2.5kg Fructus Lycii, the 2.0kg Radix Astragali and the 1.5kg Semen Cuscutae; The temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 70-72 ℃;
(2) according to the said method of step (1) medicinal residues that filter gained are carried out 3 lixiviates again, and the lixiviating solution that obtains three times respectively lixiviate produced;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure through steam in the Chinese medicine extraction equipment; The temperature of lixiviating solution is controlled at 74～76 ℃ in the concentration process, treats that the lixiviating solution weight reduction is to stop behind the 10kg concentrating and obtain concentrating lixiviating solution;
When (4) treating that concentrated lixiviating solution temperature is reduced to 60 ℃; In concentrating lixiviating solution, add dense quality degree and be 1% chitosan solution and stir after left standstill 24 hours, filter the deposition of removing in the solution then, obtain filtrating; Wherein, the weight of chitosan solution is for concentrating 15% of lixiviating solution weight.
The method for preparing of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment also comprises the steps:
A. the filtrating of step (4) gained is carried out concentrating under reduced pressure, treat that said filtrating weight adds an amount of dextrin and sucrose and mix homogeneously when being concentrated to 2.5kg and obtains mixture, the weight ratio of said dextrin and sucrose is 4: 1.
B. step a gained mixture is pushed 12 mesh sieves and dry under 65 ℃ condition, obtained the Chinese medicine composition product of granule dosage form at last.
The effect of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment gained:
270 healthy chickens of 30 ages in days are divided into three groups at random, and group I is the normal healthy controls group, and group II is the counteracting toxic substances matched group, and group III is a test group, and group II is divided into 4 groups more separately with the chicken of group III; Group I only feeds with normal feedstuff; Group II uses the normal feedstuff identical with the normal healthy controls group to feed; Not administration behind the counteracting toxic substances; Group III uses the normal feedstuff identical with the normal healthy controls group to feed, and after counteracting toxic substances the infectious bursal disease typical clinical symptom occurred in 48 hours, in test group drinking-water, adds the Chinese medicine composition that present embodiment makes, and every day, Chinese medicine composition that every chicken is joined was suitable with 1.0 gram raw material of Chinese medicine doses; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law; Observe disease chicken symptom when 9:00 and 16:00 every day, and dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and performs itemized record; Carry out dead chicken being weighed, dissecting in the 9th day to test; And record pathological changes situation, after test is accomplished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data sees Table-5:
Table-5
| Index is handled | Sick chicken number | Death toll | Cure number | Significant figure | Mortality rate (%) | Cure rate (%) | Effective percentage (%) |
| Group III | 120 | 9 | 111 | 111 | 7.5 | 92.5 | 92.5 |
| Group II | 120 | 47 | - | - | 39.2 | -- | -- |
| Group I | 0 | 0 | - | - | 0 | -- | -- |
Notes: death is all classical symptom and dead to occur at duration of test, and necropsy has bursal disease virus apparition person, is judged to infect death;
Effectively after medication, dead chicken be not effectively, and the percentage rate that every group of chicken number of surviving accounts for the test chicken number is an effective percentage;
Recovery from illness is after medication, and it is normal that the mental status, appetite etc. are recovered, and all belongs to recovery from illness, and the percentage rate that recovery from illness chicken number accounts for the test chicken number is a cure rate.
Data explanation from table: the Chinese medicine composition of the treatment infectious bursal disease that present embodiment makes can be treated infectious bursal disease effectively, and effective percentage is 92.5%, compares with the counteracting toxic substances matched group, reduces disease chicken death rate 31.7%.
To test chicken and all slaughter, pathological changes such as damage of the fabricius bursa of anatomic observation chicken and hemorrhage of muscle degree, and represent its pathological change degree with score value, relevant data is seen table-6:
Table-6
| Dissect disease chicken number | Score value (meansigma methods ± standard error) | |
| Group III | 120 | 1.147±0.824* |
| Group II | 120 | 2.117±0.593 |
| Group I | 30 | 0 |
Can find out that from last table the pathology score value of the test group chicken of administration significantly is lower than the pathology score value (P＜0.05) of counteracting toxic substances matched group chicken, show that the Chinese medicine composition product of present embodiment can obviously improve the anti-infection ability of chicken to bursal disease virus.
Need to prove that in table of present embodiment-5 and the table-6, the data of group II are the meansigma methods of its 4 group's experimental datas, the data of group III are the meansigma methods of its 4 group's experimental datas.
Embodiment four:
(1) 5.0kg Fructus Ligustri Lucidi, 2.5kg Fructus Lycii, the 1.8kg Radix Astragali and 1.7kg Semen Cuscutae being pulverized the back packs into 100kg water and carries out Steam Heating in the Chinese medicine extraction equipment; The temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 74-77 ℃;
(2) according to the said method of step (1) medicinal residues that filter gained are carried out 3 lixiviates again, and the lixiviating solution that obtains three times respectively lixiviate produced;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and concentrate through steam in the Chinese medicine extraction equipment; The temperature of lixiviating solution is controlled between 75～77 ℃ in the concentration process, treats that the lixiviating solution weight reduction stops to the 15kg concentrating and obtains concentrating lixiviating solution;
When (4) treating that concentrated lixiviating solution temperature is reduced to 60 ℃; In concentrating lixiviating solution, add mass concentration and be 1% chitosan solution and stir after left standstill 24 hours; Filter the deposition of removing in the solution then; Gained filtrating is the Chinese medicine composition of the treatment infectious bursal disease of present embodiment gained, and wherein, the weight of chitosan solution is for concentrating 15% of lixiviating solution weight.
The effect of the Chinese medicine composition of the treatment infectious bursal disease of present embodiment gained:
270 healthy chickens of 30 ages in days are divided into three groups at random, and group I is the normal healthy controls group, and group II is the counteracting toxic substances matched group, and group III is a test group, and group II is divided into 4 groups more separately with the chicken of group III; Group I only feeds with normal feedstuff; Group II uses the normal feedstuff identical with the normal healthy controls group to feed; Not administration behind the counteracting toxic substances; Group III uses the normal feedstuff identical with the normal healthy controls group to feed, and after counteracting toxic substances the infectious bursal disease typical clinical symptom occurred in 48 hours, in test group drinking-water, adds the Chinese medicine composition that present embodiment makes, and every day, Chinese medicine composition that every chicken is joined was suitable with 1.0 gram raw material of Chinese medicine doses; Three groups of chicken free choice feedings, drinking-water, Routine Management; Raise identical natural law; Observe disease chicken symptom when 9:00 and 16:00 every day, and dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and performs itemized record; Carry out dead chicken being weighed, dissecting in the 9th day to test; And record pathological changes situation, after test is accomplished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data is seen table-7
Table-7
| Index is handled | Sick chicken number | Death toll | Cure number | Significant figure | Mortality rate (%) | Cure rate (%) | Effective percentage (%) |
| Group III | 120 | 10 | 110 | 110 | 8.3 | 91.7 | 91.7 |
| Group II | 120 | 50 | - | - | 41.7 | -- | -- |
| Group I | 0 | 0 | - | - | 0 | -- | -- |
Notes: death is all classical symptom and dead to occur at duration of test, and necropsy has bursal disease virus apparition person, is judged to infect death;
Effectively after medication, dead chicken be not effectively, and the percentage rate that every group of chicken number of surviving accounts for the test chicken number is an effective percentage;
Recovery from illness is after medication, and it is normal that the mental status, appetite etc. are recovered, and all belongs to recovery from illness, and the percentage rate that recovery from illness chicken number accounts for the test chicken number is a cure rate.
Data explanation from table: the Chinese medicine composition of the treatment infectious bursal disease that present embodiment makes can be treated infectious bursal disease effectively, and effective percentage is 91.7%, compares with the counteracting toxic substances matched group, reduces disease chicken death rate 33.4%.
To test chicken and all slaughter, pathological changes such as damage of the fabricius bursa of anatomic observation chicken and hemorrhage of muscle degree, and represent its pathological change degree with score value, relevant data is seen table-8:
Table-8
| Dissect disease chicken number | Score value (meansigma methods ± standard error) | |
| Group III | 120 | 1.092±0.782* |
| Group II | 120 | 2.610±0.812 |
| Group I | 30 | 0 |
Can find out that from last table the pathology score value of the test group chicken of administration significantly is lower than the pathology score value (P＜0.05) of counteracting toxic substances matched group chicken, show that the Chinese medicine composition product of present embodiment can obviously improve the anti-infection ability of chicken to bursal disease virus.
Need to prove that in table of present embodiment-7 and the table-8, the data of group II are the meansigma methods of its 4 group's experimental datas, the data of group III are the meansigma methods of its 4 group's experimental datas.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although with reference to preferred embodiment the present invention is specified, those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention; And not breaking away from the aim and the scope of technical scheme of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (7)
1. treat the Chinese medicine composition of infectious bursal disease, it is characterized in that: crude drug is composed of the following components by weight percentage: 40-42% Fructus Ligustri Lucidi, 20-25% Fructus Lycii, 15-20% Semen Cuscutae and the 18-23% Radix Astragali.
2. the Chinese medicine composition of treatment infectious bursal disease according to claim 1 is characterized in that: said Chinese medicine composition is a granule.
3. the method for the Chinese medicine composition of the described treatment infectious bursal disease of preparation claim 1 is characterized in that: comprise the steps:
(1) said crude drug is pulverized the back and mix with the water of 8-10 times of weight and heat lixiviate, the temperature of controlling the lixiviate feed liquid is 75 ℃～80 ℃ and lixiviate 1 hour under reflux state, then material is filtered and obtains lixiviating solution and medicinal residues;
The medicinal residues that (2) will filter gained (1) said method set by step carry out 2-3 time lixiviate again and obtain the lixiviating solution of each time lixiviate generation respectively;
(3) carry out concentrating under reduced pressure after the lixiviating solution merging with step (1) and step (2) gained; The temperature of control lixiviating solution is between 70 ℃～80 ℃ in the concentration process, and the 1-2 that the weight of treating lixiviating solution is reduced to crude drug weight stops heating and obtains concentrating lixiviating solution after doubly;
(4) the concentrated lixiviating solution of step (3) gained is lowered the temperature.
4. the Chinese medicine composition method for preparing of treatment infectious bursal disease according to claim 3; It is characterized in that: in the step (4); Concentrated lixiviating solution temperature adds chitosan solution when reducing to 50 ℃～60 ℃ and stirs; Leave standstill 24h and go deposition after the temperature of waiting to contain the concentrated lixiviating solution of chitosan is reduced to room temperature, the mass concentration of said chitosan solution is 1%, and the weight that adds chitosan solution is for concentrating 10%～15% of lixiviating solution weight.
5. the method for preparing of the Chinese medicine composition of treatment infectious bursal disease according to claim 4 is characterized in that: also comprise the steps:
A. the filtrating of step (4) gained is carried out concentrating under reduced pressure, treat that adding dextrin and sucrose and mix homogeneously behind the 25-40% of said filtrating weight simmer down to crude drug weight obtains mixture;
B. step a gained mixture extruding is sieved and dry under 60-65 ℃ condition, obtain the Chinese medicine composition product of granule dosage form at last.
6. the method for preparing of the Chinese medicine composition of treatment infectious bursal disease according to claim 5 is characterized in that: among the step a, the temperature when filtrating concentrates is controlled at 70 ℃～80 ℃.
7. the method for preparing of the Chinese medicine composition of treatment infectious bursal disease according to claim 6 is characterized in that: the weight ratio of said sucrose and dextrin is 2.5-4: 1.
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| CN103550400A (en) * | 2013-11-15 | 2014-02-05 | 重庆市畜牧科学院 | Chinese medicinal composition capable of improving immunity of poultry as well as preparation and preparation method thereof |
| CN106491834A (en) * | 2016-12-09 | 2017-03-15 | 重庆市畜牧科学院 | A kind of veterinary Chinese medicinal composition and its preparation method and application of granule |
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| Title |
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| 崔保安，等.有机硒和某些中草药对鸡免疫功能的影响.《畜牧与兽医》.2003,第35卷(第10期),37-38. * |
| 石达友,等.中药提取物对鸡免疫器官发育和新城疫抗体水平的影响.《中兽医学杂志》.2004,(第2期),4-5. * |
| 马得莹，等.HIJKLMNOPQRSTUVWXYUZ[\.《动物营养学报》.2004,第16卷(第2期),36-40. * |
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