CN101671681A - 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 - Google Patents
新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 Download PDFInfo
- Publication number
- CN101671681A CN101671681A CN200910167358A CN200910167358A CN101671681A CN 101671681 A CN101671681 A CN 101671681A CN 200910167358 A CN200910167358 A CN 200910167358A CN 200910167358 A CN200910167358 A CN 200910167358A CN 101671681 A CN101671681 A CN 101671681A
- Authority
- CN
- China
- Prior art keywords
- asn
- leu
- ser
- thr
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 305
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 291
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 290
- 239000000203 mixture Substances 0.000 title claims description 27
- 239000002157 polynucleotide Substances 0.000 title description 20
- 108091033319 polynucleotide Proteins 0.000 title description 20
- 102000040430 polynucleotide Human genes 0.000 title description 20
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 title description 4
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 239
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 238
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 238
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 35
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 60
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 60
- 239000002773 nucleotide Substances 0.000 claims description 37
- 125000003729 nucleotide group Chemical group 0.000 claims description 37
- 150000001413 amino acids Chemical class 0.000 claims description 32
- 235000001014 amino acid Nutrition 0.000 claims description 26
- 239000013604 expression vector Substances 0.000 claims description 24
- 238000009396 hybridization Methods 0.000 claims description 13
- 241000726221 Gemma Species 0.000 claims description 12
- 235000013311 vegetables Nutrition 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 239000004236 Ponceau SX Substances 0.000 claims description 7
- 241000255925 Diptera Species 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 3
- 239000013543 active substance Substances 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 239000002853 nucleic acid probe Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 68
- 238000000034 method Methods 0.000 abstract description 45
- 101150078024 CRY2 gene Proteins 0.000 abstract description 17
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 5
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 770
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 768
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 666
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 631
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 525
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 439
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 337
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 315
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 314
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 301
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 253
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 249
- 241000196324 Embryophyta Species 0.000 description 119
- 210000004027 cell Anatomy 0.000 description 76
- 102220023256 rs387907547 Human genes 0.000 description 53
- 230000014509 gene expression Effects 0.000 description 50
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 39
- 108020004414 DNA Proteins 0.000 description 37
- 102220023257 rs387907546 Human genes 0.000 description 37
- 108090000623 proteins and genes Proteins 0.000 description 29
- 210000001519 tissue Anatomy 0.000 description 29
- 230000009261 transgenic effect Effects 0.000 description 28
- 230000008859 change Effects 0.000 description 26
- 125000000539 amino acid group Chemical group 0.000 description 24
- 229940024606 amino acid Drugs 0.000 description 23
- 230000008521 reorganization Effects 0.000 description 21
- 230000000875 corresponding effect Effects 0.000 description 17
- 230000000694 effects Effects 0.000 description 17
- 239000003053 toxin Substances 0.000 description 17
- 231100000765 toxin Toxicity 0.000 description 17
- 108700012359 toxins Proteins 0.000 description 17
- 238000005516 engineering process Methods 0.000 description 15
- 238000011534 incubation Methods 0.000 description 14
- 238000012216 screening Methods 0.000 description 14
- 102220369446 c.1274G>A Human genes 0.000 description 13
- 102220369445 c.668T>C Human genes 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000012634 fragment Substances 0.000 description 13
- 210000000056 organ Anatomy 0.000 description 12
- 102220023258 rs387907548 Human genes 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 11
- 230000006870 function Effects 0.000 description 9
- 230000004927 fusion Effects 0.000 description 9
- 239000013598 vector Substances 0.000 description 9
- 241000256259 Noctuidae Species 0.000 description 8
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 7
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 7
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 7
- 241000209149 Zea Species 0.000 description 7
- 230000000968 intestinal effect Effects 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 230000006798 recombination Effects 0.000 description 7
- 238000005215 recombination Methods 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 6
- 241000208125 Nicotiana Species 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 6
- 235000005911 diet Nutrition 0.000 description 6
- 230000037213 diet Effects 0.000 description 6
- 210000002706 plastid Anatomy 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 238000003259 recombinant expression Methods 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000256247 Spodoptera exigua Species 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 230000008929 regeneration Effects 0.000 description 5
- 238000011069 regeneration method Methods 0.000 description 5
- XFTWUNOVBCHBJR-UHFFFAOYSA-N Aspergillomarasmine A Chemical compound OC(=O)C(N)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O XFTWUNOVBCHBJR-UHFFFAOYSA-N 0.000 description 4
- 241000701489 Cauliflower mosaic virus Species 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 101000963974 Hydrophis stokesii Alpha-elapitoxin-Ast2b Proteins 0.000 description 4
- 101000964025 Naja naja Long neurotoxin 3 Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 241000209094 Oryza Species 0.000 description 4
- 244000062793 Sorghum vulgare Species 0.000 description 4
- 241001312519 Trigonella Species 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 235000021186 dishes Nutrition 0.000 description 4
- 238000013467 fragmentation Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 230000009545 invasion Effects 0.000 description 4
- 238000007899 nucleic acid hybridization Methods 0.000 description 4
- 235000011056 potassium acetate Nutrition 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 241000219194 Arabidopsis Species 0.000 description 3
- 241001148495 Cibotium barometz Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 241000219146 Gossypium Species 0.000 description 3
- 241001300479 Macroptilium Species 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 3
- 108700001094 Plant Genes Proteins 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 235000002634 Solanum Nutrition 0.000 description 3
- 241000207763 Solanum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 235000021405 artificial diet Nutrition 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 229930182817 methionine Natural products 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 230000008488 polyadenylation Effects 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000009871 tenuigenin Substances 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 2
- 241000607534 Aeromonas Species 0.000 description 2
- 241000589158 Agrobacterium Species 0.000 description 2
- 241000234282 Allium Species 0.000 description 2
- 235000003840 Amygdalus nana Nutrition 0.000 description 2
- 244000296825 Amygdalus nana Species 0.000 description 2
- 235000001271 Anacardium Nutrition 0.000 description 2
- 241000693997 Anacardium Species 0.000 description 2
- 244000099147 Ananas comosus Species 0.000 description 2
- 235000003911 Arachis Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- 241001106067 Atropa Species 0.000 description 2
- 235000005781 Avena Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 241000589154 Azotobacter group Species 0.000 description 2
- 241001112741 Bacillaceae Species 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 241000339490 Brachyachne Species 0.000 description 2
- 241000131971 Bradyrhizobiaceae Species 0.000 description 2
- 241000218236 Cannabis Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 235000013913 Ceratonia Nutrition 0.000 description 2
- 241001060815 Ceratonia Species 0.000 description 2
- 241000219312 Chenopodium Species 0.000 description 2
- 241000219109 Citrullus Species 0.000 description 2
- 241000207199 Citrus Species 0.000 description 2
- 241000723377 Coffea Species 0.000 description 2
- 241000209205 Coix Species 0.000 description 2
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 2
- 244000024469 Cucumis prophetarum Species 0.000 description 2
- 241000219122 Cucurbita Species 0.000 description 2
- 241000208296 Datura Species 0.000 description 2
- 241000208175 Daucus Species 0.000 description 2
- 241000605716 Desulfovibrio Species 0.000 description 2
- 240000001879 Digitalis lutea Species 0.000 description 2
- 244000281702 Dioscorea villosa Species 0.000 description 2
- 235000000504 Dioscorea villosa Nutrition 0.000 description 2
- 241000588921 Enterobacteriaceae Species 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000221079 Euphorbia <genus> Species 0.000 description 2
- 241000234642 Festuca Species 0.000 description 2
- 241000218218 Ficus <angiosperm> Species 0.000 description 2
- 241000220223 Fragaria Species 0.000 description 2
- 241000208152 Geranium Species 0.000 description 2
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 235000009438 Gossypium Nutrition 0.000 description 2
- 241000208818 Helianthus Species 0.000 description 2
- 235000005206 Hibiscus Nutrition 0.000 description 2
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 description 2
- 244000284380 Hibiscus rosa sinensis Species 0.000 description 2
- 241000209219 Hordeum Species 0.000 description 2
- 102000004310 Ion Channels Human genes 0.000 description 2
- 235000021506 Ipomoea Nutrition 0.000 description 2
- 241000207783 Ipomoea Species 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 241001468155 Lactobacillaceae Species 0.000 description 2
- 241000208822 Lactuca Species 0.000 description 2
- 241000219729 Lathyrus Species 0.000 description 2
- 241000234435 Lilium Species 0.000 description 2
- 241000208204 Linum Species 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- 241000219745 Lupinus Species 0.000 description 2
- 241000227653 Lycopersicon Species 0.000 description 2
- 241000220225 Malus Species 0.000 description 2
- 241001093152 Mangifera Species 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 241000219823 Medicago Species 0.000 description 2
- 241000234295 Musa Species 0.000 description 2
- 241000234479 Narcissus Species 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 2
- 241000036147 Ochlerotatus taeniorhynchus Species 0.000 description 2
- 241000795633 Olea <sea slug> Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241000209117 Panicum Species 0.000 description 2
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 2
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 2
- 241000208181 Pelargonium Species 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 241000218196 Persea Species 0.000 description 2
- 241000219833 Phaseolus Species 0.000 description 2
- 241000607568 Photobacterium Species 0.000 description 2
- 241000218657 Picea Species 0.000 description 2
- 235000005205 Pinus Nutrition 0.000 description 2
- 241000218602 Pinus <genus> Species 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 235000011432 Prunus Nutrition 0.000 description 2
- 241000218683 Pseudotsuga Species 0.000 description 2
- 241000508269 Psidium Species 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 2
- 241000219492 Quercus Species 0.000 description 2
- 241000218206 Ranunculus Species 0.000 description 2
- 241000589180 Rhizobium Species 0.000 description 2
- 241000208422 Rhododendron Species 0.000 description 2
- 235000003846 Ricinus Nutrition 0.000 description 2
- 241000322381 Ricinus <louse> Species 0.000 description 2
- 241000209051 Saccharum Species 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 241000780602 Senecio Species 0.000 description 2
- 241000607720 Serratia Species 0.000 description 2
- 235000005775 Setaria Nutrition 0.000 description 2
- 241000232088 Setaria <nematode> Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 241000253368 Spirillaceae Species 0.000 description 2
- 241000605008 Spirillum Species 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- 241000723873 Tobacco mosaic virus Species 0.000 description 2
- 108700019146 Transgenes Proteins 0.000 description 2
- 241000255993 Trichoplusia ni Species 0.000 description 2
- 241000219793 Trifolium Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 239000006035 Tryptophane Substances 0.000 description 2
- 241000722923 Tulipa Species 0.000 description 2
- 241000607598 Vibrio Species 0.000 description 2
- 241000219977 Vigna Species 0.000 description 2
- 235000009392 Vitis Nutrition 0.000 description 2
- 241000219095 Vitis Species 0.000 description 2
- 241000588901 Zymomonas Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 244000193174 agave Species 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 229940043431 ceratonia Drugs 0.000 description 2
- 235000019504 cigarettes Nutrition 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 241001233957 eudicotyledons Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229960002989 glutamic acid Drugs 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 235000019713 millet Nutrition 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000006320 pegylation Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000006337 proteolytic cleavage Effects 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 235000014774 prunus Nutrition 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 229960004799 tryptophan Drugs 0.000 description 2
- -1 ttacks Species 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
- 101150062057 ANR1 gene Proteins 0.000 description 1
- 235000007173 Abies balsamea Nutrition 0.000 description 1
- 244000283070 Abies balsamea Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000218473 Agrotis Species 0.000 description 1
- 241000208306 Apium Species 0.000 description 1
- 101100215339 Arabidopsis thaliana ACT11 gene Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000209128 Bambusa Species 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000255794 Bombyx mandarina Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 241000209200 Bromus Species 0.000 description 1
- 101100342815 Caenorhabditis elegans lec-1 gene Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 240000001548 Camellia japonica Species 0.000 description 1
- 235000014649 Carica monoica Nutrition 0.000 description 1
- 244000132069 Carica monoica Species 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- WLYGSPLCNKYESI-RSUQVHIMSA-N Carthamin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@]1(O)C(O)=C(C(=O)\C=C\C=2C=CC(O)=CC=2)C(=O)C(\C=C\2C([C@](O)([C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(O)=C(C(=O)\C=C\C=3C=CC(O)=CC=3)C/2=O)=O)=C1O WLYGSPLCNKYESI-RSUQVHIMSA-N 0.000 description 1
- 241000208809 Carthamus Species 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 108020004998 Chloroplast DNA Proteins 0.000 description 1
- 235000010521 Cicer Nutrition 0.000 description 1
- 241000220455 Cicer Species 0.000 description 1
- 241000737241 Cocos Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 241000020428 Colea Species 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 101710151559 Crystal protein Proteins 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 241000209210 Dactylis Species 0.000 description 1
- 240000004585 Dactylis glomerata Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 240000003421 Dianthus chinensis Species 0.000 description 1
- 235000005903 Dioscorea Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000512897 Elaeis Species 0.000 description 1
- 235000001942 Elaeis Nutrition 0.000 description 1
- 235000001950 Elaeis guineensis Nutrition 0.000 description 1
- 244000127993 Elaeis melanococca Species 0.000 description 1
- 108010013369 Enteropeptidase Proteins 0.000 description 1
- 102100029727 Enteropeptidase Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 108010074860 Factor Xa Proteins 0.000 description 1
- 101150062467 GAT gene Proteins 0.000 description 1
- 101150030514 GPC1 gene Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 240000004859 Gamochaeta purpurea Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 101150094780 Gpc2 gene Proteins 0.000 description 1
- 241000255990 Helicoverpa Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000256257 Heliothis Species 0.000 description 1
- 244000043261 Hevea brasiliensis Species 0.000 description 1
- 241000208278 Hyoscyamus Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- 241000219739 Lens Species 0.000 description 1
- 240000004322 Lens culinaris Species 0.000 description 1
- 235000010666 Lens esculenta Nutrition 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000208467 Macadamia Species 0.000 description 1
- 240000007575 Macadamia integrifolia Species 0.000 description 1
- 235000018330 Macadamia integrifolia Nutrition 0.000 description 1
- 235000003800 Macadamia tetraphylla Nutrition 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 241000121629 Majorana Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 101100217138 Mus musculus Actr10 gene Proteins 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241001162910 Nemesia <spider> Species 0.000 description 1
- 241000207746 Nicotiana benthamiana Species 0.000 description 1
- 241001585712 Noctua Species 0.000 description 1
- 241001330001 Olyreae Species 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 241001330025 Pharoideae Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 101000870887 Phaseolus vulgaris Glycine-rich cell wall structural protein 1.8 Proteins 0.000 description 1
- 241000746981 Phleum Species 0.000 description 1
- 241000543704 Pistacia Species 0.000 description 1
- 241001092090 Pittosporum Species 0.000 description 1
- 241000209048 Poa Species 0.000 description 1
- 244000292693 Poa annua Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000947836 Pseudomonadaceae Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 241001552694 Rhizobacter Species 0.000 description 1
- 241001633102 Rhizobiaceae Species 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- 235000011483 Ribes Nutrition 0.000 description 1
- 241000220483 Ribes Species 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 241001116459 Sequoia Species 0.000 description 1
- 241001138418 Sequoia sempervirens Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- 241000543826 Sinobambusa Species 0.000 description 1
- 101150019148 Slc7a3 gene Proteins 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 244000087212 Stenotaphrum Species 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 241000218685 Tsuga Species 0.000 description 1
- YJQCOFNZVFGCAF-UHFFFAOYSA-N Tunicamycin II Natural products O1C(CC(O)C2C(C(O)C(O2)N2C(NC(=O)C=C2)=O)O)C(O)C(O)C(NC(=O)C=CCCCCCCCCC(C)C)C1OC1OC(CO)C(O)C(O)C1NC(C)=O YJQCOFNZVFGCAF-UHFFFAOYSA-N 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- ZVNYJIZDIRKMBF-UHFFFAOYSA-N Vesnarinone Chemical compound C1=C(OC)C(OC)=CC=C1C(=O)N1CCN(C=2C=C3CCC(=O)NC3=CC=2)CC1 ZVNYJIZDIRKMBF-UHFFFAOYSA-N 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 108010036419 acyl-(acyl-carrier-protein)desaturase Proteins 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Chemical compound BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000012219 cassette mutagenesis Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 229920003211 cis-1,4-polyisoprene Polymers 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 235000018597 common camellia Nutrition 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000004879 dioscorea Nutrition 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000022244 formylation Effects 0.000 description 1
- 238000006170 formylation reaction Methods 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 108020002326 glutamine synthetase Proteins 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000005497 microtitration Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000007826 nucleic acid assay Methods 0.000 description 1
- 210000002221 olecranon process Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000020245 plant milk Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000000614 rib Anatomy 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000007888 toxin activity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- ZHSGGJXRNHWHRS-VIDYELAYSA-N tunicamycin Chemical compound O([C@H]1[C@@H]([C@H]([C@@H](O)[C@@H](CC(O)[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C(NC(=O)C=C2)=O)O)O1)O)NC(=O)/C=C/CC(C)C)[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(C)=O ZHSGGJXRNHWHRS-VIDYELAYSA-N 0.000 description 1
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 231100000925 very toxic Toxicity 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明提供了与芽孢杆菌Cry2多肽相关的杀虫多肽。也提供了编码本发明多肽的核酸。包括使用本发明多肽和核酸提高植物对昆虫捕食抗性的方法。
Description
本申请是国际申请日为2005年2月25日的国际申请PCT/US2005/006238进入中国、申请号为200580011701.4的题为“新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物”的发明专利申请的分案申请。
1.技术领域
本发明总的来说涉及害虫控制领域并提供了与芽孢杆菌Cry2多肽相关的杀虫多肽和编码它们的多核苷酸。本发明还涉及改变植物对昆虫捕食抗性的方法和组合物,包括但不限于转基因植物生产。
2.背景技术
许多商业上有价值的植物,包括普通农作物,易受昆虫和线虫害虫攻击。这些害虫可以引起农作物产量和质量大幅度降低。传统上,农场主严重依靠化学杀虫剂与害虫损害作斗争。然而,化学杀虫剂的使用引起了其自身的一系列问题,包括应用杀虫剂的成本和不便。此外,化学残留物引起了环境和健康忧虑。对于这些和其它理由,需要选择性的杀虫剂。
控制害虫的对环境无害方法是使用来源于土壤细菌苏云金芽孢杆菌(Bacillus thuringiensis)(“Bt”)的杀虫晶体蛋白,通常称为“Cry蛋白”。许多这些蛋白对具体靶昆虫十分有毒性,但是对植物和其它非靶生物无害。一些Cry蛋白已在农作物植物中重组表达以提供害虫抗性转基因植物。在这些当中,Bt转基因棉花和玉米已被广泛耕种。
许多Cry蛋白已被分离、表征和基于氨基酸序列同源性进行分类(Crickmore等人,1998,Microbiol.Mol.Biol.Rev.,62:807-813)。这个分类方案提供了对新发现的Cry蛋白进行命名和分类的系统机制。
通常发现除Cry2A之外,单个Cry蛋白具有相对窄的活性谱。Cry2A与众不同,因为Cry蛋白的这个亚型具有较宽的有效范围,包括对昆虫鳞翅目(Lepidoptera)和双翅目(Diptera)的毒性。发现Cry2A蛋白是显示出抗粉纹夜蛾(Trichoplusia ni)(粉纹夜蛾)和带喙伊蚊(Aedes taeniorhynchus)(蚊子)双重活性的毒素(Yamamoto和McLaughlin,1982,Biochem.Biophys.Res.Comm.130:414-421)。编码Cry2A蛋白的核酸分子(术语为Cry2Aa)被克隆和在巨大芽孢杆菌(B.megaterium)中表达,并被发现具有抗鳞翅目和双翅目昆虫的活性(Donovan等人,1988,J.Bacteriol.170:4732-4738)。与Cry2Aa同源的另外编码序列(术语为Cry2Ab)被克隆并发现仅仅有对抗鳞翅目幼虫的活性(Widner和Whiteley,1989,J Bacteriol 171:2)。
如果第二代转基因农作物能够表达多个和/或新的Bt基因,则它们可能对昆虫更有抗性。因此,具有宽的活性谱的新杀虫蛋白将非常合乎需要。
3.发明内容
本发明涉及新的Cry2多肽-Cry2Ax(SEQ ID NO:2),其从苏云金芽孢杆菌分离。本发明还包括Cry2Ax衍生多肽(SEQ ID NOS:4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260)。除Cry2Ax和Cry2Ax衍生多肽的多肽序列之外,人们会认识到本发明的多肽还包括其变体,包括但不限于其任何片段、类似物、同源物、天然存在的等位基因或突变体。本发明的多肽还包括由本发明的任何Cry2Ax或Cry2Ax衍生核酸编码的那些多肽。在一个实施方案中,多肽具有至少一种Cry2Ax功能活性(例如杀虫活性)且与SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260或其变体的任何一个多肽序列具有至少85%、90%、95%、97%、98%或99%的同一性。在另一个实施方案中,包括具有至少一种Cry2Ax功能活性(例如杀虫活性),长度为至少25、50、75、100、125、150、175、200、225、250、275、300、325、350、375、400、425、450、475、500、525、550、575、600或625个邻接氨基酸,并由在严格条件下与编码SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260或其变体的任何一个的核酸杂交的多核苷酸编码的多肽。还提供了本发明多肽的制备方法,例如通过重组方法。还包括包含一个或多个本发明多肽的组合物。
本发明还涉及Cry2Ax的核酸分子(SEQ ID NO:1)和Cry2Ax衍生核酸分子(SEQ ID NOS:3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259)。本发明还包括编码具有至少部分功能活性,即它们能够显示与野生型Cry2Ax多肽相关的一种或多种已知功能活性的多肽的片段和类似物。在一个实施方案中,本发明包括包含下列的核苷酸序列的分离的核酸分子:i)与SEQ ID NOS:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259的任何一个的核苷酸序列具有至少70%、75%、80%、85%、90%、95%、97%、98%或99%的同一性的核苷酸序列;ii)与由SEQ ID NOS:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259或其互补物的任何一个的核苷酸序列组成的核酸探针在严格条件下杂交的核苷酸序列;和/或iii)包含编码包含SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个的氨基酸序列的多肽的核酸分子的核苷酸序列。还包括包含本发明核酸的载体。还包括包含本发明载体的细胞或植物。
本发明还涉及表达本发明核酸和/或多肽的转基因植物。转基因植物可以以本领域任何已知的方法表达转基因,包括但不限于组成型表达、发育调节的表达、组织特异性表达等。还包括从本发明转基因植物获得的种子。
4.附图说明
图1显示了来自第二轮改组的DNA克隆的杀虫活性。每个克隆都使用强制渗入在本萨明那烟(N.benthamiana)叶中表达。将每个叶盘供应给单一的三龄谷实夜蛾(H.zea)幼虫。24小时温育期后,通过视觉观察确定摄食活力并以剩余叶面积的近似分数来表示。y轴是接触昆虫后,剩余叶盘的百分比。x轴是叶盘中表达的克隆。几个克隆显示出杀虫活性增加,如7K(D_S01000779)(SEQ ID NO:10)、15K(D_S00999080)(SEQ ID NO:12)、16K(D_S01000269)(SEQ IDNO:14)、16R(D_S01037143)(SEQ ID NO:16)和473R(D_S01037677)(SEQ ID NO:18)。
图2显示了第一轮改组的克隆44(D_S00503970)和第三轮改组的克隆D_S01764701的杀虫活性。每个克隆都使用强制渗入在本萨明那烟叶中表达。将每个叶盘供应给单一的三龄谷实夜蛾幼虫。24小时温育期后,通过叶盘视频捕获确定摄取活力。y轴是捕获的叶盘图像中存在的像素数目。x轴是叶盘中表达的克隆。结果显示的是三个实验的平均值。对于每个实验,每个克隆测试至少八个叶盘。
图3A-3B显示了在质体室(左组)或细胞质(右组)中表达第一轮改组的克隆44的转基因烟草植物的功效结果。在叶与虫温育24小时后测定(A)谷实夜蛾或(B)甜菜夜蛾(S.exigua)抑制的功效。用真实计算相对剩余叶面积(像素数目)的视频捕获设备观察剩余叶量。每个转基因植物取六个叶盘进行分析。因为使用每个转基因构建体制备了二十五个转基因植物,所以数字区别使用特定构建体的不同植物。
图4A-4B显示了表达第一轮改组的克隆44的转基因植物中的转基因表达水平。这个改组的Cry2衍生多肽通过分别用pMAXY5469或pMAXY5471转化而在(A)质体或(B)细胞质亚细胞室中表达。使用针对第一轮改组的克隆44多肽的毒素区的多克隆抗体,在转基因植物提取物上进行蛋白印迹分析。阴性对照是从未转化植物中获取的提取物。阳性对照是20ng或40ng纯化的Cry2Ax毒素。阳性对照Cry2Ax的分子量不同于植物提取物中Cry2Ax衍生多肽的分子量,因为前者是胰蛋白酶活化的和后者是前毒素。
5.具体实施方式
本发明提供了与芽孢杆菌Cry2多肽相关的杀虫多肽。还提供了编码本发明多肽的核酸分子。包括使用本发明的多肽和核酸来增强植物对昆虫捕食的抗性的方法。
5.1本发明的多肽
本发明涉及新的Cry2多肽-Cry2Ax(SEQ ID NO:2),其从苏云金芽孢杆菌分离。本发明还包括Cry2Ax衍生多肽(SEQ ID NOS:4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260)。本发明的多肽还包括由任何本发明Cry2Ax或Cry2Ax衍生核酸编码的那些多肽(参见5.2节)。
除Cry2Ax和Cry2Ax衍生多肽的多肽序列之外,人们会认识到本发明的多肽还包括其变体,包括但不限于其任何基本上相似的序列、任何片段、类似物、同源物、天然存在的等位基因或突变体。本发明包括的变体是至少具有部分功能活性的,即它们能够显示出与野生型Cry2Ax多肽相关的一种或多种已知功能活性的多肽。这种功能活性包括但不限于生物学活性,如杀虫活性;抗原性,即结合抗Cry2Ax抗体或与Cry2Ax竞争结合抗Cry2Ax抗体的能力;免疫原性,即产生结合Cry2Ax多肽的抗体的能力。在一些实施方案中,该变体具有至少一种基本上类似于其亲本多肽的功能活性(例如Cry2Ax变体将具有至少一种基本上类似于Cry2Ax的功能活性)。如在此使用的变体的功能活性将被认为“基本上类似于”其亲本多肽,如果它在亲本一个标准差(onestandard deviation)以内的话。
在一个实施方案中,具有至少一种Cry2Ax功能活性(如杀虫活性)且与SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个的多肽序列具有至少85%、90%、95%、97%、98%或99%同一性的多肽包括在本发明中。本发明的这种多肽含有来自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少1个、至少5个、至少10个、至少20个、至少30个或全部40个氨基酸残基。下标指出了多肽序列与SEQ ID NO:2最佳比对后,与SEQ ID NO:2中位置对应的氨基酸残基位置。至于与参照序列最佳比对的氨基酸序列,氨基酸“对应于”比对中参照序列中的残基所配对的位置。
如在此使用的,在序列被定义为与参照序列具有“至少X%同一性”,例如“与SEQ ID NO:2具有至少95%同一性的多肽”的情况下,应理解“X%同一性”是指绝对百分比同一性,除非另有陈述。术语“绝对百分比同一性”是指通过将相同氨基酸或核酸计分为1和将任何取代计分为0,不考虑错配氨基酸或核酸的相似性所确定的序列同一性百分比。在一种一般的序列比对中,两个序列的“绝对百分比同一性”表示为氨基酸或核酸“同一性”的百分比。如果两个序列的最佳比对需要在一个或两个序列中插入缺口,则为了测定百分比同一性,与另一个序列中缺口比对的一个序列中的氨基酸残基被计数为错配。缺口可以是内部或外部的,即截短。使用例如Clustal W程序,1.8版本,1999年6月,使用缺省参数可以容易地测定绝对百分比同一性(Thompson等人,1994,Nucleic Acids Research 22:4673-4680)。
在另一个实施方案中,Cry2Ax和Cry2Ax衍生多肽的片段包括在本发明中。包括具有至少一种Cry2Ax功能活性(例如杀虫活性),长度是SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个的至少25、50、75、100、125、150、175、200、225、250、275、300、325、350、375、400、425、450、475、500、525、550、575、600或625个邻接氨基酸,且由在严格条件下与编码SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个的核酸杂交的多核苷酸编码的多肽。在本发明片段包括对应于SEQ IDNO:2的氨基酸残基2、7、27、35、36、43、44、45、51、58、69、78、79、99、118、124、125、129、138、139、141、161、165、166、183、192、211、213、217、218、324、386、399、405、445、551、587、591、610、631的任何氨基酸残基的实施方案中,本发明的这种多肽含有来自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少1个、至少5个、至少10个、至少20个、至少30个或全部40个氨基酸残基。
在具体实施方案中,本发明的片段对应于加工过的前毒素长度。全长前毒素Cry2和加工过的Cry2毒素之间分子量的差异是5-6kDa。这是~40个氨基酸被从前毒素Cry2多肽切割的结果(Rukmini等人,2000,Biochimie 82:109-116;Aronson等人,1993,Mol.Microbiol.7:489-496;Morse等人,2001,Structure 9:409-17)。本发明的方法可以直接提供与这个加工过的Cry2片段对应的多肽,以绕开前毒素加工的需要。
在另一个具体实施方案中,本发明的片段对应于Cry2结构域。Cry2多肽具有三个结构域,包括i)参与插入昆虫顶端中肠膜和影响离子通道功能的结构域I;ii)参与受体结合于昆虫中肠上皮细胞膜上的结构域II,和iii)参与离子通道功能、受体结合和插入膜的结构域III (Dean等人,1996,Gene 179:111-117;Schnepf等人,1998,Microbiol.Molec.Biol.Rev.62:775-806)。
在另一个实施方案中,类似多肽包括在本发明中。类似多肽可以具有已经被修饰的残基,即通过任何类型分子与Cry2Ax或Cry2Ax衍生多肽的共价附着。例如,但并不作为限制,本发明的类似多肽可以例如通过糖基化、乙酰化、聚乙二醇化(pegylation)、磷酸化、酰胺化、被已知保护/封闭基团衍生化、蛋白酶剪切、与细胞配体或其它蛋白连接等等而被修饰。本发明的类似多肽可以被化学修饰而修饰,其中使用本领域技术人员已知的技术,包括但不限于特异性化学切割、乙酰化、甲酰化、衣霉素代谢合成等等。此外,本发明多肽的类似物可以含有一个或多个非经典的氨基酸。
还提供了本发明多肽的制备方法,例如通过重组方法(参见5.6节)。
还包括包含一个或多个本发明多肽的组合物。本发明的组合物可以进一步包含另外的试剂,包括但不限于涂布器-粘着剂佐剂、稳定剂、其它杀虫添加剂、稀释剂、最优化组合物流变性质或稳定性的试剂,例如表面活性剂、乳化剂、分散剂和/或聚合物。
5.2本发明的核酸
本发明还涉及Cry2Ax的核酸分子(SEQ ID NO:1)和Cry2Ax衍生核酸分子(SEQ ID NOS:3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259)。本发明的核酸分子还包括编码本发明的任何Cry2Ax或Cry2Ax衍生多肽的那些核酸分子(参见5.1节)。
除Cry2Ax和Cry2Ax衍生核酸分子的核酸分子之外,人们会认识到本发明的核酸还包括其变体,包括但不限于其任何基本上相似的序列、任何片段、同源物、天然存在的等位基因或突变体。本发明包括的变异核酸分子编码至少具有部分功能活性的,即它们能够显示出与野生型Cry2Ax多肽相关的一种或多种已知功能活性的多肽。这种功能活性包括但不限于生物学活性,如杀虫活性;抗原性,即结合抗Cry2Ax抗体或与Cry2Ax竞争结合抗Cry2Ax抗体的能力;免疫原性,即产生结合Cry2Ax多肽的抗体的能力。在一些实施方案中,该变体具有至少一种基本上类似于其亲本核酸分子的功能活性(例如Cry2Ax核酸分子变体将编码具有至少一种基本上类似于Cry2Ax核酸分子编码的多肽的功能活性的多肽)。如在此使用的变体的功能活性将被认为“基本上类似于”其亲本多肽,如果它在亲本一个标准差以内的话。
在一个实施方案中,与SEQ ID NOS:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259任何一个核酸分子具有至少70%、75%、80%、85%、90%、95%、97%、98%或99%同一性的核酸分子包括在本发明中。本发明的这种核酸分子编码含有来自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少1个、至少5个、至少10个、至少20个、至少30个或全部40个氨基酸残基的多肽。
为测定两个核酸分子的百分比同一性,为了最佳比较目的,将序列进行比对(例如为了与第二个或核酸分子进行最佳比对,可以在第一个核酸分子序列中引入缺口)。然后比较相应核苷酸位置的核苷酸。当第一个序列中的位置被与第二个序列相应位置相同的核苷酸占用时,那么在那个位置分子是相同的。两个序列之间的百分比同一性是序列共享有的相同位置的数目的函数(即,同一性%=相同重叠位置数目/位置总数目x100%)。在一个实施方案中,两个序列是相同长度。
两个序列之间百分比同一性的测定还可以使用数学算法完成。比较两个序列所用的数学算法的非限制性实例是Karlin和Altschul的算法(Karlin和Altschul,1990,Proc.Natl.Acad.Sci.87:2264-2268,如在Karlin和Altschul,1993,Proc.Natl.Acad.Sci.90:5873-5877所改进的)。这种算法合并入NBLAST和XBLAST程序(Altschul等人,1990,J.Mol.Biol.215:403和Altschul等人,1997,Nucleic Acid.Res.25:3389-3402)。进行BLAST分析的软件是公众可得到的,例如通过国家生物技术信息中心(National Center for BiotechnologyInformation)。这个算法包括首先通过在查询序列中鉴定长度W的短字,鉴定高得分序列对(HSPs),所述字与数据库序列中相同长度的字比对时,匹配或满足一些阳性值阈得分T。T被认为是邻近字得分阈(Altschul等人,前述)。这些最初的邻近字命中充当启动研究以发现更长含有它们的HSPs的种子。字命中然后沿着每个序列双向延伸,只要累积比对得分可以增加。对于核苷酸序列,使用参数M(一对匹配残基的奖励得分;总是>0)和N(错配残基罚分;总是<0)计算累积得分。对于氨基酸序列,使用得分矩阵计算累积得分。当下列情况时,停止字命中在每个方向的延伸:累积比对得分从其最大所得值降低量X;由于一个或多个负得分残基比对的累积,累积得分到达零或更低;或到达每个序列的末尾。BLAST算法参数W、T和X测定比对的灵敏度和速度。BLASTN程序(对于核苷酸序列)使用如下默认值:字长(W)为11、期望(E)为10、截止值(cutoff)为100、M=5、N=4和两条链的比较。对于氨基酸序列,BLASTP程序使用字长(W)为3、期望(E)为10和BLOSUM62得分矩阵的默认值(参见Henikoff & Henikoff,1989,PNAS,89:10915)。
也可以使用比对的Clustal V方法测定百分比同一性(Higgins和Sharp,1989,CABIOS.5:151-153)且其可在LASERGENE生物信息计算程序组(DNASTAR Inc.,Madison,WI)的Megalign程序中找到。“默认参数”是程序制造商预先设置的参数,并且对于多重比对,它们对应于缺口惩罚(GAP PENALTY)=10和缺口长度惩罚(GAPLENGTH PENALTY)=10,而对于成对比对,它们是KTUPLE 1、缺口惩罚(GAP PENALTY)=3、窗口(WINDOW)=5和DIAGONALSSAVED=5。序列比对后,使用Clustal V程序,通过观察相同程序上的“序列距离”表可能获得“百分比同一性”。
两个序列之间百分比同一性可以使用类似于如上所述的那些技术测定,其中允许或不允许缺口。在计算百分比同一性中,一般仅仅计数精确匹配的。
在另一个实施方案中,Cry2Ax和Cry2Ax衍生核酸分子的片段包括在本发明中。包括具有至少一种Cry2Ax功能活性(例如杀虫活性),长度是SEQ ID NOS:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259任何一个的至少100、250、500、750、1000、1500或1800个邻接核苷酸,和/或在严格条件下与编码SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个的核酸分子杂交的核酸分子。在本发明核酸片段编码包含对应于SEQ ID NO:2的氨基酸残基2、7、27、35、36、43、44、45、51、58、69、78、79、99、118、124、125、129、138、139、141、161、165、166、183、192、211、213、217、218、324、386、399、405、445、551、587、591、610、631的任何氨基酸残基的多肽的实施方案中,本发明的这种核酸分子含有来自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少1个、至少5个、至少10个、至少20个、至少30个或全部40个氨基酸残基的编码序列。
在一个具体实施方案中,本发明的片段对应于编码加工过的前毒素的核酸长度。全长前毒素Cry2和加工过的Cry2毒素之间的分子量差异是5-6kDa。这是~40个氨基酸被从前毒素Cry2多肽切割的结果(Rukmini等人,2000,Biochimie 82:109-116;Aronson等人,1993,Mol.Microbiol.7:489-496;Morse等人,2001,Structure 9:409-17)。
在另一个具体实施方案中,本发明的片段编码对应于Cry2结构域的多肽。
在另一个实施方案中,在严格条件下与SEQ ID NOS:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、159、161、163、165、167、169、171、173、175、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215、217、219、221、223、225、227、229、231、233、235、237、239、241、243、245、247、249、251、253、255、257、259的任何一个杂交的核酸分子包括在本发明中。本发明的这种核酸分子编码含有来自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少1个、至少5个、至少10个、至少20个、至少30个或全部40个氨基酸残基的多肽。
短语“严格条件”是指核酸将与它的靶核酸杂交的杂交条件,一般在核酸的复杂混合物中,但是基本上没有其它核酸。严格条件是依赖于序列的并且在不同的环境下将不同。较长的核酸在较高的温度下特异性杂交。核酸杂交的广泛指导可以在现有技术中找到(例如Tijssen,Techniques in Biochemistry and MolecularBiology--Hybridization with Nucleic Probes,“Overview of principlesof hybridization and the strategy of nucleic acid assays”(1993))。通常,高严格条件选择为比限定离子强度pH下具体核酸的热解链温度(Tm)低大约5-10℃。低严格条件通常选择为低于Tm大约15-30℃。Tm是(在限定离子强度、pH和核酸浓度下)与靶互补的50%探针与靶核酸在平衡状态中杂交的温度(由于靶核酸过量存在,在Tm,50%探针在平衡状态被占用)。杂交条件一般是pH 7.0至8.3下,盐浓度小于约1.0M钠离子,一般约0.01至1.0M钠离子浓度(或其它盐)和对于短探针(例如10至50个核苷酸)温度至少约30℃且对于长探针(例如大于50个核苷酸)温度至少约60℃的条件。严格条件还可以通过添加去稳定剂如甲酰胺达到。对于选择性或特异性杂交,阳性信号是至少两倍于背景,和优选10倍于背景杂交。在一个实施方案中,严格条件包括在0.2X SSC,至少约50℃,通常约55℃,或有时60℃或65℃的温度下至少洗涤一次(通常2次),洗涤20分钟,或基本上等同的条件。在具体实施方案中,本发明的核酸分子在0.2X SSC、55℃洗涤一次,洗涤20分钟后,与编码SEQ ID NOS:2、4、6、8、10、12、14、16、18、20、22、24、26、28、30、32、34、36、38、40、42、44、46、48、50、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132、134、136、138、140、142、144、146、148、150、152、154、156、158、160、162、164、166、168、170、172、174、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214、216、218、220、222、224、226、228、230、232、234、236、238、240、242、244、246、248、250、252、254、256、258、260任何一个多肽的多核苷酸特异性杂交。在另一个实施方案中,严格条件包括在6X氯化钠/柠檬酸钠(SSC),在约45℃杂交,随后在0.2XSSC,0.1%SDS,50-65℃洗涤一次或多次。
短语“特异性杂交”是指在严格杂交条件下,一种分子仅仅与特殊核苷酸序列结合、形成双链或杂交,当那个序列在复杂混合物(例如全细胞或文库DNA或RNA)中存在的话。
还包括包含本发明核酸的载体。还包括包含本发明载体的细胞或植物。
术语“核酸”或“核酸分子”在此是指从5’至3’末端阅读的脱氧核糖核苷酸或核糖核苷酸碱基的单链或双链聚合物。它包括染色体DNA、自我复制质粒和执行一级结构角色的DNA或RNA。术语“编码”是指编码一个或多个氨基酸的多核苷酸序列。该术语不需要起始或终止密码子。氨基酸序列可以由多核苷酸序列及其互补物提供的六个不同读框的任何一个编码。
表1公开了Cry2Ax和Cry2Ax衍生序列和相应的序列标识编号。
5.3Cry2Ax衍生序列
本发明的Cry2Ax衍生多肽和核酸可以通过如下方法生成,将一个或多个核苷酸取代、添加和/或缺失引入Cry2Ax或相关核酸的核苷酸序列,以致于一个或多个氨基酸取代、添加和/或缺失被引入编码的蛋白。通常,为了强调Cry2Ax多肽合乎需要的特征或减少不合需要的特征,产生Cry2Ax衍生序列。在一个实施方案中,Cry2Ax衍生多肽具有比Cry2Ax提高的杀虫活性,包括但不限于更大的效力和/或害虫范围增加。在另一个实施方案中,Cry2Ax衍生多肽比Cry2Ax更好表达,包括但不限于半衰期增加、对降解易感性更低和/或更有效转录或翻译。
在一个实施方案中,将Cry2Ax(SEQ ID NO:1)核酸分子用作模板以产生Cry2Ax衍生核苷酸。在另一个实施方案中,将Cry2Ax相关核酸用作模板以产生Cry2Ax衍生核苷酸。在具体实施方案中,将Cry2Ab*用作模板。Cry2Ab*相对于野生型Cry2Ab具有两个氨基酸改变(GenBank登录号M23724的36位K变为R和241位M变为T)。在另一个具体实施方案中,从一轮改变分离的克隆被用作模板进行下一轮改变(例如克隆38、44和473R;参见6.2和6.4节)。
序列改变可以通过标准技术来引入,如定向分子进化技术,例如DNA改组方法(参见例如Christians等人,1999,Nature Biotechnology17:259-264;Crameri等人,1998,Nature,391:288-291;Crameri,等人,1997,Nature Biotechnology 15:436-438;Crameri等人,1996,Nature Biotechnology 14:315-319;Stemmer,1994,Nature 370:389-391;Stemmer等人,1994,Proc.Natl.Acad.Sci.,91:10747-10751;美国专利号5,605,793;6,117,679;6,132,970;5,939,250;5,965,408;6,171,820;国际公开号WO 95/22625;WO 97/0078;WO 97/35966;WO 98/27230;WO 00/42651和WO 01/75767);定点诱变(参见例如Kunkel,1985,Proc.Natl.Acad.Sci.,82:488-492;Oliphant等人,1986,Gene44:177-183);寡核苷酸指导的诱变(参见例如Reidhaar-Olson等人,1988,Science 241:53-57);化学诱变(参见例如Eckert等人,1987,Mutat.Res.178:1-10);易错PCR(参见例如Caldwell & Joyce,1992,PCR Methods Applic.2:28-33);和盒式诱变(参见例如Arkin等人,Proc.Natl.Acad.Sci.,1992,89:7871-7815);(通常参见例如Arnold,1993,Curr.Opinion Biotechnol.4:450-455;Ling等人,1997,Anal.Biochem.,254(2):157-78;Dale等人,1996,Methods Mol.Biol.57:369-74;Smith,1985,Ann.Rev.Genet.19:423-462;Botstein等人,1985,Science,229:1193-1201;Carter,1986,Biochem.J.237:1-7;Kramer等人,1984,Cell 38:879-887;Wells等人,1985,Gene 34:315-323;Minshull等人,1999,Current Opinion in Chemical Biology 3:284-290)。
在一个实施方案中,使用DNA改组产生Cry2Ax衍生核苷酸。DNA改组可以在体外、体内、在硅片上(in silico)或其组合进行。可进行在硅片上的重组方法,其中遗传算法用在电脑中重组对应于同源(乃至非同源的)核酸的序列串。所得的重组序列串通过合成对应于重组序列的核酸被任选转变为核酸,例如与寡核苷酸合成基因重装配技术协同。这个方法可以产生随机的、部分随机的或有计划的改变。本领域描述了关于在硅片上重组的许多细节,包括遗传算法、遗传算子等等在计算机系统中的使用,其与相应核酸以及有计划的核酸组合的产生(例如基于交换位点选择)以及有计划的、伪随机的或随机的重组方法相结合(参见例如国际公开号WO 00/42560和WO 00/42559)。
在另一个实施方案中,使用定向诱变产生Cry2Ax衍生核苷酸,其通过选择Cry2Ax或相关核酸的特定核苷酸序列或位置进行改变来进行。这种定向突变可以引入核酸中的任何位置。例如,人们可以进行在“非必需”或“必需”氨基酸残基引起氨基酸取代的核苷酸取代。“非必需”氨基酸残基是可以从野生型序列发生改变,但是不改变生物活性的残基,而“必需”氨基酸残基是多肽的至少一种生物活性所需的。例如,各物种同源物中不保守或仅仅半保守的氨基酸残基对于活性可能是非必需的。换句话说,各物种同源物中保守的氨基酸残基可能是活性必需的。
这种定向突变可以是保守的或非保守的。“非保守氨基酸取代”是氨基酸残基被具有不相似侧链的氨基酸残基取代的取代。本领域已经定义了具有相似侧链的氨基酸残基家族。这些家族包括具有碱性侧链(例如赖氨酸、精氨酸、组氨酸)、酸性侧链(例如天冬氨酸、谷氨酸、天冬酰胺、谷氨酰胺)、不带电荷极性侧链(例如甘氨酸、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、非极性侧链(例如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、β支链侧链(例如苏氨酸、缬氨酸、异亮氨酸)和芳族侧链(例如酪氨酸、苯丙氨酸、色氨酸、组氨酸)的氨基酸。
替代地或除非保守氨基酸残基取代外,这种定向突变可以是保守的。“保守氨基酸取代”是氨基酸残基被具有相似侧链的氨基酸残基取代的取代。诱变后,编码的蛋白可以被重组表达且可以测定蛋白活性。
在另一个实施方案中,使用随机诱变产生Cry2Ax衍生核苷酸。突变可以沿编码序列的全部或部分随机引入(例如通过饱和诱变)。在某些实施方案中,编码具有相似结构域、结构基序、活性位点,或与本发明Cry2Ax的一部分比对具有错配或不完全配对的其它相关多肽的核苷酸序列可以用在诱变处理中以产生序列多样性。
应该理解对于上面提及的一些技术的每个诱变步骤,可以执行任何或全部步骤的许多反复循环以最优化序列多样性。上述方法可以以任何所需顺序组合。在许多情况下,该方法产生改变的核酸序列库或包含改变的核酸序列的重组宿主细胞的库。改变的核酸序列或表达具有所需特征的改变的核酸序列的宿主细胞可以用本领域已知的一个或多个测定筛选而鉴定。该测定可以在为具有所需物理或化学特性的多肽所选择的条件下进行。核酸序列中的改变可以通过对克隆中编码改变的多肽的核酸分子测序来测定。
另外,Cry2Ax和Cry2Ax衍生核酸分子可以被密码子最优化,其中全部或部分被最优化。因为任何一个氨基酸(除了甲硫氨酸)都由许多密码子(表2)编码,所以核酸分子的序列可以改变,而不改变编码的氨基酸。密码子最优化是当一个或多个密码子在核酸水平改变时,使得氨基酸不改变,但是在特定宿主生物中表达增加。本领域普通技术人员将认识到提供各种各样生物的偏爱信息的表和其它参考文献在本领域是可以获得的。
5.4测定杀虫活性的方法
如在此使用的术语“杀虫活性”是指在摄取多肽后多肽降低或抑制昆虫摄食和/或增加昆虫死亡率的能力。尽管任何昆虫都可以受到作用,但是优选作用于鳞翅目和双翅目昆虫。
可以使用各种测定来测定本发明的特定多肽是否具有杀虫活性和,倘若如此,到何种程度。通常,本发明的多肽以可以被摄取的任何形式提供给害虫。观察害虫摄取本发明多肽的反应(例如约一至三天)。摄取本发明多肽后,摄食的降低或抑制和/或害虫死亡率增加是杀虫活性的指示。具有未知杀虫活性的本发明多肽应该与阳性和/或阴性对照比较来更正确估计测定结果。
在一个实施方案中,本发明的多肽被纯化(以可溶形式或晶体形式)并添加到昆虫饮食中。
在另一个实施方案中,本发明多肽在重组微生物(例如大肠杆菌(E.coli))中表达。直接向害虫饲喂重组微生物(参见Moellenbeck等人,2001,Nat.Biotechnol.19:668)。
在另一个实施方案中,本发明的多肽在植物中表达和将植物喂给害虫。温育期后,害虫的摄食活性可以通过正常已被害虫吃掉的植物部分的视觉观察(例如占剩余叶面积的近似分数)或视频捕获(例如剩余叶面积中像素的数目)来测定。在具体实施方案中,本发明多肽在植物中的表达短暂。在这种实施方案中,编码本发明多肽的核酸被克隆入植物表达载体并转染入根癌土壤杆菌(Agrobacterium tumefaciens)。转化的细菌与本萨明那烟的叶子共同培养,并使用强制渗入,叶子表达本发明的多肽。然而,叶共同培养物之间多肽的表达是可变的。在另一个具体实施方案中,本发明多肽在植物中的表达是稳定的。在这种实施方案中,制备表达本发明多肽的转基因植物。
在另一个实施方案中,本发明多肽的杀虫活性可以通过使用培养细胞测量细胞死亡和/或细胞生长来测定。这种测定一般包括使用对筛选的特定毒素易感的培养的昆虫细胞,或天然地或作为表达异源基因的结果表达特定毒素的受体的细胞。因此,除昆虫细胞之外,哺乳动物、细菌和酵母细胞在对体外测定有用的那些细胞当中。本领域描述了测量抗培养的细胞的毒性的体外生物测定法(例如Johnson,1994,J.Invertebr.Pathol.63:123-129)。
在另一个实施方案中,本发明多肽的杀虫活性可以通过测量靶昆虫衍生的中肠上皮膜囊的孔形成来测定(Juttner和Ebel,1998,Biochim.Biophys.Acta 1370:51-63.;English等人,1991,Insect Biochem.21:177-184)。这种测定可以形成来自膜囊腔的配体活化底物的毒素条件性释放。这需要配体处于囊泡外面上。可供选择地,可以使用相反方案,其中配体在囊腔中和待活化的底物位于囊外面。毒素活性越高,形成孔的数目或大小越高。
5.5提高植物昆虫抗性的方法
本发明提供了通过使用Cry2相关杀虫多肽提高植物对害虫抗性的方法,所述害虫包括但不限于实夜蛾属物种(Helicoverpa ssp.)(例如谷实夜蛾)和/或夜蛾属物种(Spodoptera ssp.)(例如甜菜夜蛾)。本领域已知的任何方法可用于引起害虫在摄食植物过程中摄取一个或多个本发明多肽。同样,害虫将摄取一个或多个本发明多肽的杀虫量和可以中止摄食植物。在一些实施方案中,通过摄取本发明的一个或多个多肽杀死害虫。在其它实施方案中,害虫被阻止或阻拦摄食植物,而不是被杀死。
在一个实施方案中,可以制备转基因植物以表达本发明的一个或多个多肽(通常参见5.7节转基因植物的制备方法)。转基因植物可以在全部组织中表达本发明的一个或多个多肽(例如全局表达)。可供选择地,本发明的一个或多个多肽可以仅在组织亚型中表达(例如组织特异性表达),优选害虫消耗的那些组织。本发明的多肽可以在植物中组成型表达或在诱导型启动子控制下表达。
在另一个实施方案中,包含一个或多个本发明多肽的组合物可以从外部应用于对害虫易感的植物。组合物的外部应用包括直接应用于植物,全部或部分地,和/或间接应用,例如应用于植物周围的环境如土壤。可以用本领域已知的任何方法应用组合物,包括但不限于喷雾、撒粉、喷洒等等。通常,可以在植物生长过程中的任何时间应用组合物。本领域技术人员可以使用本领域已知方法凭经验确定施用组合物的最佳时间。影响最佳施用时间的因素包括但不限于易感植物的类型、害虫类型、哪个或哪些本发明多肽在组合物中施用。
包含本发明一个或多个多肽的组合物可以是基本上纯化的多肽、细胞悬浮液、细胞沉淀、细胞上清液、细胞提取物或苏云金芽孢杆菌细胞的芽孢晶体复合物(通常参见5.6节重组多肽合成技术)。包含本发明一个或多个多肽的组合物可以是溶液、乳剂、悬浮液或粉末形式。液体制剂可以是基于水或非水的,和可以作为泡沫、凝胶、悬浮液、可乳化浓缩物等等提供。该制剂可以包括除本发明一个或多个多肽之外的试剂。例如,该组合物可以进一步包含涂布器-粘着剂佐剂、稳定剂、其它杀虫添加剂、稀释剂、最优化组合物流变性质或稳定性的试剂,例如表面活性剂、乳化剂、分散剂或聚合物。
在另一个实施方案中,表达本发明一个或多个多肽的重组宿主可以应用在对害虫攻击易感的植物上或附近。重组宿主包括但不限于已经被本发明一个或多个核酸分子(和因此多肽)转化并表达所述分子的微生物宿主和昆虫病毒。在一些实施方案中,重组宿主将本发明多肽分泌到它的周围环境,从而接触害虫。在其它实施方案中,重组宿主定居于对昆虫侵袭易感的一个或多个植物组织。
5.6重组表达
使用本领域熟知的标准重组DNA和分子克隆技术可以重组表达本发明的核酸分子和多肽(例如Sambrook,Fritsch,和Maniatis,Molecular Cloning:A Laboratory Manual;Cold Spring HarborLaboratory Press:Cold Spring Harbor,1989)。另外,可以使用重组DNA技术产生适用于制备转基因植物的核酸构建体(参见5.7节)。
因此,本发明的一个方面涉及载体,优选表达载体,其包含本发明的核酸分子或其变体。如在此使用的术语“载体”是指能够运输已与其连接的另一个核酸的多核苷酸。一种类型的载体是“质粒”,其是指其中可以引入另外的DNA片段的环状双链DNA环。另一类型的载体是病毒载体,其中另外的DNA片段可以引入病毒基因组。
某些载体能够在引入它们的宿主细胞中自主复制(例如具有细菌复制起点的细菌载体和游离型载体)。其它载体(例如非游离型载体)在引入宿主细胞后被整合到宿主细胞基因组中,且因此与宿主基因组一起复制。通常,重组DNA技术中利用的表达载体常常是质粒(载体)的形式。然而,本发明意欲包括这种其它形式的表达载体,如病毒载体(例如复制缺陷型逆转录病毒)。
本发明的重组表达载体包含本发明的核酸分子,其形式适于在宿主细胞中表达所述核酸分子。这意思指重组表达载体包括基于用来表达的宿主细胞所选择的一个或多个调节序列,其与待表达的多核苷酸可操作地结合。在重组表达载体内,“可操作地结合”意图是指目标核苷酸序列与调节序列以允许所述核苷酸序列表达(例如当载体引入宿主细胞时,在体外转录/翻译系统或在宿主细胞中)的方式连接。术语“调节序列”意图是包括启动子、增强子和其它表达控制元件(例如多腺苷酸化信号)。本领域描述了这种调节序列(例如Goeddel,GeneExpression Technology:Methods in Enzymology,1990,AcademicPress,San Diego,CA)。调节序列包括指导核苷酸序列在许多类型宿主细胞中组成型表达的序列和指导核苷酸序列仅在某些宿主细胞中表达的序列(例如组织特异性调节序列)。本领域技术人员将领会到表达载体的设计可以取决于诸如待转化宿主细胞的选择、所需的蛋白表达水平、所需表达的生物区域等等的因素。本发明的表达载体可以被引入宿主细胞,以由此生产蛋白或肽,包括融合蛋白或肽,其由在此描述的核酸分子编码。
在一些实施方案中,充当启动子或增强子元件的分离的核酸可以被引入非异源形式的本发明多核苷酸的合适位置(通常上游),以致于上调或下调本发明多核苷酸的表达。例如,内源启动子可以通过突变、缺失和/或取代而体内改变(参见美国专利号5,565,350;国际专利申请号PCT/US93/03868),或分离的启动子可以以适当的方向和与本发明多核苷酸的同源基因适当的距离引入植物细胞,以致于控制基因的表达。基因表达可以在适于植物生长的条件下调节,以致于改变总浓度和/或改变植物细胞中本发明多肽的组成。因此,本发明提供了组合物和制备可操作地连接于天然、内源(即非异源)形式的本发明多核苷酸的异源启动子和/或增强子的方法。
如果需要多肽表达,则在多核苷酸编码区3’末端包括多腺苷酸化区通常是合乎需要的。多腺苷酸化区可以源自于天然基因、来自各种其它植物基因或来自T-DNA。待添加的3′末端序列可以源自于例如胭脂碱合酶或章鱼肉碱合酶基因,或者可选择地来自另一个植物基因,或较低优选地来自任何其它真核基因。
本发明的重组表达载体可以设计成在原核生物中表达本发明的多肽(例如肠杆菌科(Enterobacteriaceae),如埃希氏菌属(Escherichia);芽胞杆菌科(Bacillaceae);根瘤菌科(Rhizoboceae),如根瘤菌属(Rhizobium)和根瘤杆菌属(Rhizobacter);螺菌科(Spirillaceae),如发光杆菌属(Photobacterium);发酵单胞菌属(Zymomonas);沙雷氏菌属(Serratia);气单胞菌属(Aeromonas);弧菌属(Vibrio);脱硫弧菌属(Desulfovibrio);螺菌属(Spirillum);乳杆菌科(Lactobacillaceae);假单胞菌科(Pseadomonadaceae),如假单胞菌属(Pseudomonas)和醋杆菌属(Acetobacter);固氮菌科(Azotobacteraceae)和硝化杆菌科(Nitrobacteraceae))或真核细胞(例如使用杆状病毒表达载体的昆虫细胞、酵母细胞、植物细胞或哺乳动物细胞)(参见Goeddel,前述,关于合适宿主细胞的讨论)。可供选择地,重组表达载体可以在体外转录和翻译,例如使用T7启动子调节序列和T7聚合酶。
原核生物中蛋白的表达最经常在具有包含指导融合或非融合蛋白表达的组成型或诱导型启动子的载体的大肠杆菌中进行。融合载体向在其中编码的蛋白中添加许多氨基酸,通常添加至重组蛋白的氨基末端。这种融合载体一般提供至少三个目的:1)增加重组蛋白的表达;2)增加重组蛋白的溶解度;和/或3)通过在亲和纯化中充当配体帮助重组蛋白纯化。常常,在融合表达载体中,在融合部分和重组蛋白的接点引入蛋白酶剪切位点,以使得在融合蛋白纯化后,重组蛋白能够从融合部分分离。这种酶和它们的同源识别序列包括因子Xa、凝血酶和肠激酶。一般的融合表达载体包括pGEX(Pharmacia Biotech Inc;Smith和Johnson,1988,Gene 67:31-40)、pMAL(New England Biolabs,Beverly,MA)和pRIT5(Pharmacia,Piscataway,NJ),所述载体分别使谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白或A蛋白与靶重组蛋白融合。
在另一个实施方案中,表达载体是酵母表达载体。啤酒糖酵母(S.cerevisiae)中表达的载体的实例包括pYepSec1(Baldari等人,1987,EMBO J.6:229-234)、pMFa(Kurjan和Herskowitz,1982,Cell30:933-943)、pJRY88(Schultz等人,1987,Gene 54:113-123)、pYES2(Invitrogen Corp.,San Diego,CA)和pPicZ(Invitrogen Corp.,SanDiego,CA)。
可供选择地,表达载体是杆状病毒表达载体。在培养的昆虫细胞(例如Sf9细胞)中可表达蛋白的杆状病毒载体包括pAc系列(Smith等人,1983,Mol.Cell Biol.3:2156-2165)和pVL系列(Lucklow和Summers,1989,Virology 170:31-39)。
在又一个实施方案中,使用植物表达载体在植物细胞中表达本发明的核酸,所述载体包括但不限于烟草花叶病毒和马铃薯病毒表达载体。
原核和真核细胞的其它合适表达系统是本领域已知的(参见例如Sambrook等人,1990,Molecular Cloning,A Laboratory Manual,2d Ed.,Cold Spring Harbor Laboratory,Cold Spring Harbor,NY的第16和17章)。
许多启动子可以用在本发明的实践中。可以基于所需结果选择启动子。核酸可以与组成型、组织特异性、诱导型或其它启动子组合以在宿主生物中表达。
“组织特异性启动子”可以指导本发明核酸在具体组织、器官或细胞类型中表达。组织特异性启动子可以是诱导型的。类似地,组织特异性启动子可以仅仅促进在那些组织内某一时间范围或发育阶段内的转录。其它组织特异性启动子可以贯穿具体组织的生活周期都具有活性。本领域普通技术人员将认识到组织特异性启动子可以驱动与其可操作地连接的序列在除了靶组织之外的组织中的表达。因此,如在此使用的,组织特异性启动子是优选驱动在靶组织或细胞类型中的表达,但是也可以导致其它组织中的一些表达的启动子。许多组织特异性启动子可以用在本发明中。利用合适的启动子,任何器官可以被靶向,如枝条营养器官/结构(例如叶、茎和块茎)、根、花和花器官/结构(例如苞、萼片、花瓣、雄蕊、心皮、花药和胚珠)、种子(包括胚、胚乳和种皮)和果实。例如,指导在叶、根或花中的核酸表达的启动子可用于提高对感染那些器官的害虫的抗性。对于在植物气生营养器官中表达本发明的多核苷酸,可以使用光合作用器官特异性启动子如RBCS启动子(Khoudi等人,Gene 197:343,1997)。本发明多核苷酸的根特异性表达可以在根特异性启动子控制下完成,如例如来自ANR1基因的启动子(Zhang和Forde,Science,279:407,1998)。其它示例的启动子包括来自大豆的根特异性谷氨酰胺合成酶基因(Hirel等人,1992,Plant Molecular Biology 20:207-218)和法国菜豆GRP 1.8基因中的根特异性控制元件(Keller等人,1991,The Plant Cell 3:1051-1061)。
“组成型启动子”定义为将指导基因在所有组织中表达和在大多数环境条件和发育或细胞分化状态下有活性的的启动子。组成型启动子的实例包括花椰菜花叶病毒(CaMV)35S转录起始区、来源于根癌土壤杆菌T-DNA的1’或2’启动子和本领域普通技术人员已知的来自各种植物基因的其它转录起始区。这种基因包括例如来自拟南芥属(Arabidopsis)的ACT11(Huang等人,1996,Plant Mol.Biol.33:125-139)、来自拟南芥属的Cat3(GenBank登录号U43147,Zhong等人,1996,Mol.Gen.Genet.251:196-203)、来自欧洲油菜(Brassicanapus)的编码硬脂酰-酰基载体蛋白去饱和酶的基因(Genbank登录号X74782,Solocombe等人,1994,Plant Physiol.104:1167-1176),来自玉米的GPc1(GenBank登录号X15596,Martinez等人,1989,J.Mol.Biol.208:551-565)和来自玉米的Gpc2(GenBank登录号U45855,Manjunath等人,1997,Plant Mol.Biol.33:97-112)。任何强烈的组成型启动子如CaMV 35S启动子都可以用于本发明多核苷酸贯穿植物的表达。
术语“诱导型启动子”是指在精确环境或发育控制下的启动子。可以影响诱导型启动子转录的环境条件的实例包括厌氧条件、升高的温度、光的存在或用化学试剂/激素喷雾。
用于植物宿主细胞的合适组成型启动子包括例如Rsyn7启动子的核心启动子和其它相关组成型启动子(国际公开号WO 99/43838和美国专利号6,072,050);核心CaMV 35S启动子(Odell等人,1985,Nature313:810-812);稻肌动蛋白(McElroy等人,1990,Plant Cell 2:163-171);遍在蛋白(Christensen等人,1989,Plant Mol.Biol.12:619-632和Christensen等人,1992,Plant Mol.Biol.18:675-689);pEMU(Last等人,1991,Theor.Appl.Genet.81:581-588);MAS(Velten等人,1984,EMBO J.3:2723-2730);ALS启动子(美国专利号5,659,026)等等(例如美国专利号5,608,149;5,608,144;5,604,121;5,569,597;5,466,785;5,399,680;5,268,463;5,608,142和6,177,611)。
本发明的另一个方面涉及已引入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此可互换使用。应理解这种术语不仅涉及特定的主题细胞,而且涉及这种细胞的子代或潜在子代。因为由于突变或者环境影响在后续世代中可能发生某些修饰,所以这种子代实际上可能与亲代细胞不相同,但是仍然包括在在此使用的术语范围内。
因此,本发明提供了具有包含本发明核酸或其变体的表达载体的宿主细胞。宿主细胞可以是任何原核生物(例如大肠杆菌、苏云金芽孢杆菌)或真核细胞(例如昆虫细胞、酵母或植物细胞)。本发明还提供了表达本发明核酸,由此制备所编码的多肽的方法,其包括如下步骤:i)在允许生产所编码多肽的条件下培养包含本发明核酸分子的细胞;和ii)分离表达的多肽。
载体DNA可通过传统的转化或转染技术引入原核或真核细胞。如在此使用的术语“转化”和“转染”意欲指将外来核酸分子引入宿主细胞的各种本领域认可的技术,包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖介导的转染、脂质转染法或电穿孔。本领域可以找到转化或转染宿主细胞的合适方法(例如Sambrook,等人,前述)。
5.7转基因植物的制备
本领域已知的任何方法都可以用来用本发明的核酸分子转化植物或植物细胞。核酸分子可以整合入植物DNA(例如基因组DNA或叶绿体DNA)或维持不插入植物DNA(例如通过使用人工染色体)。将核酸分子引入植物细胞的合适方法包括显微注射(Crossway等人,1986,Biotechniques 4:320-334);电穿孔(Riggs等人,1986,Proc.Natl.Acad.Sci.83:5602-5606;D′Halluin等人,1992,Plant Cell 4:1495-1505);土壤杆菌介导的转化(美国专利号5,563,055和5,981,840,Osjoda等人,1996,Nature Biotechnology 14:745-750;Horsch等人,1984,Science233:496-498,Fraley等人,1983,Proc.Natl.Acad.Sci.80:4803,和Gene Transfer to Plants,Potrykus,ed.,Springer-Verlag,Berlin 1995);直接基因转移(Paszkowski等人,1984,EMBO J.3:2717-2722);冲击颗粒加速(ballistic particle acceleration)(美国专利号4,945,050;5,879,918;5,886,244;5,932,782;Tomes等人,1995,“Direct DNATransfer into Intact Plant Cells via Microprojectile Bombardment,inPlant Cell,Tissue,and Organ Culture:Fundamental Methods,ed.Gamborg and Phillips,Springer-Verlag,Berlin;和McCabe等人,1988,Biotechnology 6:923-926);病毒介导的转化(美国专利号5,889,191、5,889,190、5,866,785、5,589,367和5,316,931);花粉转化(DeWet等人,1985,in The Experimental Manipulation of Ovule Tissues,ed.Chapman等人,Longman,New York,pp.197-209);Lec 1转化(美国专利申请系列号09/435,054;国际公开号WO 00/28058);whisker介导的转化(Kaeppler等人,1990,Plant Cell Reports 9:415-418;Kaeppler等人,1992,Theor.Appl.Genet.84:560-566);和叶绿体转化技术(Bogorad,2000,Trends in Biotechnology 18:257-263;Ramesh等人,2004,Methods Mol Biol.274:301-7;Hou等人,2003,TransgenicRes.12:111-4;Kindle等人,1991,Proc.Natl.Acad.Sci.88:1721-5;Bateman和Purton,2000,Mol Gen Genet.263:404-10;Sidorov等人,1999,Plant J.19:209-216)。
用来产生转基因植物和植物细胞的转化方案的选择可以根据靶向转化的植物或植物细胞类型,即单子叶植物或双子叶植物而变化。特别适于特殊植物类型的转化方案实例包括对于马铃薯(Tu等人,1998,Plant Molecular Biology 37:829-838;Chong等人,2000,TransgenicResearch 9:71-78);大豆(Christou等人,1988,Plant Physiol.87:671-674;McCabe等人,1988,Bio Technology 6:923-926;Finer和McMullen,1991,In Vitro Cell Dev.Biol.27P:175-182;Singh等人,1998,Theor.Appl.Genet.96:319-324);玉米(Klein等人,1988,Proc.Natl.Acad.Sci.85:4305-4309;Klein等人,1988,Biotechnology6:559-563;Klein等人,1988,Plant Physiol.91:440-444;Fromm等人,1990,Biotechnology 8:833-839;Tomes等人,1995,“Direct DNATransfer into Intact Plant Cells via Microprojectile Bombardment,”in Plant Cell,Tissue,和Organ Culture:Fundamental Methods,ed.Gamborg(Springer-Verlag,Berlin));谷类(Hooykaas-Van Slogteren等人,1984,Nature 311:763-764;美国专利号5,736,369)的那些。
在一些实施方案中,在产生转基因植物和植物细胞中,使用一个以上构建体进行转化。多个构建体可以以顺式或反式位置包括。在优选实施方案中,每个构建体都具有启动子和其它调节序列。
可以培养通过任何上述转化技术获得的转化植物细胞以产生具有转化基因型和由此具有所需表型的完整植物。这种再生技术依靠某些植物激素在组织培养生长培养基中的操作,一般依靠已与所需核苷酸序列一起引入的杀生物剂和/或除草剂标记。本领域描述了从培养的原生质体的植物再生(例如Evans等人,Protoplasts Isolation and Culture,Handbook of Plant Cell Culture,pp.124-176,MacMillilanPublishing Company,New York,1983;和Binding,Regeneration ofPlants,Plant Protoplasts,pp.21-73,CRC Press,Boca Raton,1985)。还可以从植物愈伤组织、外植体、器官或其部分获得再生。本领域也描述了这种再生技术(例如Klee等人,1987,Ann.Rev.of Plant Phys.38:467-486)。
术语“植物”包括完整植物、枝条营养器官/结构(例如叶、茎和块茎)、根、花和花器官/结构(例如苞、萼片、花瓣、雄蕊、心皮、花药和胚珠)、种子(包括胚、胚乳和种皮)和果实(成熟子房)、植物组织(例如维管组织、基本组织等等)和细胞(例如保卫细胞、卵细胞、毛状体等等)和其子代。本发明方法中可以使用的植物类别包括可进行转化的高等和低等植物,包括被子植物(单子叶和双子叶植物)、裸子植物、蕨类植物和多细胞藻类。还包括各种倍性水平的植物,包括非整倍体、多倍体、二倍体、单倍体和半合子植物。
本发明的核酸可以用于给基本上任何植物赋予所需性状。因此,本发明使用了广泛的植物,包括来自Agrotis、葱属(Allium)、凤梨属(Ananas)、腰果属(Anacardium)、Apium、落花生属(Arachis)、天门冬属(Asparagus)、Athamantha、颠茄属(Atropa)、燕麦属(Avena)、箣竹属(Bambusa)、甜菜属(Beta)、芸苔属(Brassica)、雀麦属(Bromus)、Browaalia、山茶属(Camelia)、大麻属(Cannabis)、番木瓜属(Carica)、长角豆属(Ceratonia)、鹰嘴豆属(Cicer)、藜属(Chenopodium)、Chicorium、柑桔属(Citrus)、西瓜属(Citrullus)、辣椒属(Capsicum)、红蓝花属(Carthamus)、椰子属(Cocos)、咖啡属(Coffea)、薏苡属(Coix)、香瓜属(Cucumis)、南瓜属(Cucurbita)、狗牙根属(Cynodon)、鸭茅属(Dactylis)、曼陀罗属(Datura)、胡萝卜属(Daucus)、石竹属(Dianthus)、毛地黄属(Digitalis)、薯蓣属(Dioscorea)、油棕属(Elaeis)、Eliusine、大戟属(Euphorbia)、羊茅属(Festuca)、榕属(Ficus)、草莓属(Fragaria)、老鹳草属(Geranium)、大豆属(Glycine)、禾本科(Graminae)、棉属(Gossypium)、向日葵属(Helianthus)、Heterocallis、Hevea、木槿属(Hibiscus)、大麦属(Hordeum)、天仙子属(Hyoscyamus)、番薯属(Ipomoea)、莴苣属(Lactuca)、山黧豆属(Lathyrus)、兵豆属(Lens)、百合属(Lilium)、亚麻属(Linum)、黑麦草属(Lolium)、百脉根属(Lotus)、羽扇豆属(Lupinus)、番茄属(Lycopersicon)、澳洲坚果属(Macadamia)、Macrophylla、苹果属(Malus)、芒果属(Mangifera)、木薯属(Manihot)、Majorana、苜蓿属(Medicago)、芭蕉属(Musa)、水仙属(Narcissus)、Nemesia、烟草属(Nicotiana)、驴食豆属(Onobrychis)、木犀榄属(Olea)、Olyreae、稻属(Oryza)、稷属(Panicum)、稷属(Panicum)、Panieum、Pannisetum、狼尾草属(Pennisetum)、碧冬茄属(Petunia)、天竺葵属(Pelargonium)、鳄梨属(Persea)、Pharoideae、菜豆属(Phaseolus)、梯牧草属(Phleum)、云杉属(Picea)、早熟禾属(Poa)、松属(Pinus)、黄连木属(Pistachia)、豌豆属(Pisum)、杨属(Populus)、黄杉属(Pseudotsuga)、梨属(Pyrus)、李属(Prunus)、Pseutotsuga、番石榴属(Psidium)、栎属(Quercus)、毛莨属(Ranunculus)、萝卜属(Raphanus)、条藨子属(Ribes)、蓖麻属(Ricinus)、杜鹃花属(Rhododendron)、蔷薇属(Rosa)、甘蔗属(Saccharum)、Salpiglossis、黑麦属(Secale)、千里光属(Senecio)、狗尾草属(Setaria)、北美红杉属(Sequoia)、白芥属(Sinapis)、茄属(Solanum)、蜀黍属(Sorghum)、钝叶草属(Stenotaphrum)、Theobromus、胡芦巴属(Trigonella)、车轴草属(Trifolium)、胡芦巴属(Trigonella)、小麦属(Triticum)、铁杉属(Tsuga)、郁金香属(Tulipa)、野蚕豆属(Vicia)、葡萄属(Vitis)、豇豆属(Vigna)和玉蜀黍属(Zea)的物种。
在具体实施方案中,转基因植物是玉米、马铃薯、稻、大豆或棉花植物。
转基因植物可以与相同转化的株系或不同的株系生长和授粉。可以生长两代或更多代植物以确保稳定维持和遗传所需核酸分子、多肽和/或表型特征的表达。本领域普通技术人员将认识到本发明核酸分子稳定整合入转基因植物和证实可操作后,它可以通过有性杂交引入其它植物。可以使用众多标准育种技术的任何一种,这取决于待杂交的物种。
5.8转基因植物中表达的测定
可以使用本领域已知的任何方法测定本发明的核酸分子或其编码的多肽在植物中的表达水平。例如,本发明核酸分子编码的多肽在植物中的表达水平可以通过免疫测定、定量凝胶电泳等等测定。另外,本发明核酸分子编码的多肽在植物中的表达水平可以通过植物表型改变的程度来测定。在具体实施方案中,提高的对昆虫抗性是待测定的表型。
如在此使用的“提高的对昆虫抗性”是指表达本发明多肽的转基因植物与不表达本发明多肽的植物相比,对害虫消耗和/或侵袭的抗性增加。可以用很多方法测量提高的抗性。在一个实施方案中,通过相同昆虫温育时间段后,表达本发明多肽的植物与不表达本发明多肽的植物相比降低的损害来测量提高的抗性。昆虫损害可以以视觉估计。例如在棉花植物中,侵袭后损害可以通过直接观察棉花植物棉蒴被昆虫消耗的迹象来测量。在另一个实施方案中,通过相同昆虫温育时间段后,表达本发明多肽的植物与不表达本发明多肽的植物相比增加的农作物产量来测量提高的抗性。在特定实施方案中,害虫来自鳞翅目和/或双翅目。
可以使用完整植物、其组织或植物细胞培养物进行测定。
序列表(命名为21194286PC.TXT)是0.97MB且创建于2005年2月24日的一同递交的光盘,其全部引入作为参考。
因此在此引用的所有公开的文章、书、参考手册和摘要的内容完全引入作为参考,以更充分描述本发明相关的技术状态。
由于上述主题可以进行各种改变而不背离本发明的范围和精神,因此意图是上述说明书中含有的,和/或在所附权利要求中限定的所有主题都被解释为本发明的描述和说明。根据上述教导,本发明的改变和变型是可能的。
6.实施例
6.1实施例1:初步昆虫筛选
初步昆虫筛选从具有抗实夜蛾属物种活性的生物多样性收集物中鉴定出Bt培养物。筛选用芽孢晶体复合物样品以抗谷实夜蛾新生幼虫的高剂量实施。
芽孢晶体复合物样品制备于含有1ml CYS芽孢形成培养基的深孔制备板中(Yamamoto,1990,Analytical Chemistry of Bacillusthuringiensis 432:46-60)。使制备板接种已在-80℃冷冻的10μl种子培养物并在30℃、350rpm温育3天,直到大多数培养物形成芽孢并释放游离芽孢和晶体。将板以4000rpm离心40分钟以沉淀芽孢、晶体和未溶解的细胞。将沉淀的芽孢晶体复合物悬浮于含有100μg溶菌酶的1.2ml 15mM乙酸钾中并在30℃、250rpm温育16小时以确保完全芽孢形成和细胞溶解。温育16小时后,通过离心收集芽孢晶体复合物并悬浮于15mM乙酸钾中。这个乙酸钾步骤重复一次。在1ml 15mM乙酸钾中制备最后的芽孢晶体悬浮液并用于谷实夜蛾活性的筛选。
在每孔含有150μl人工昆虫饮食的浅96孔板中进行昆虫筛选。将20μl芽孢-晶体悬浮液放置于昆虫饮食中。每孔放置约5个新生幼虫。使昆虫测定板在29℃温育3天。昆虫对Bt晶体的反应包括摄食抑制和死亡率。约400个培养物显示出相当大死亡率并因此鉴定为阳性的。Cry2Ax在阳性当中。
6.2实施例2:DNA改组以分离Cry2Ax衍生多肽
以Cry2Ax多肽(SEQ ID NO:2)和Cry2Ab*多肽(Cry2Ab*相对于野生型Cry2Ab具有2个氨基酸改变;GenBank登录号M23724的36位K变为R和241位M变为T)开始,产生合成DNA模板进行DNA改组。使用Cry2Ab(Gen Bank登录号M23724)和Cry2Ax(SEQ IDNO:2)的种系发生比较,生成改变了这两个多肽序列之间不同的40个氨基酸位置(参见5.1节)的文库。使用寡核苷酸指导的改组产生改组的DNA文库,其中使用编码Cry2Ax的合成基因作为亲本DNA模板。将PCR DNA文库克隆入pMAXY3219,这通过取代Cry2Ab*基因实现。建立毒素克隆,从而它们以与大肠杆菌麦芽糖结合蛋白(MBP)的融合体表达。初步昆虫筛选鉴定了活跃抗实夜蛾属物种的培养物。如下实施筛选,通过将少量表达MBP::Cry2Ax衍生多肽融合体的大肠杆菌培养物以低剂量掺入人工饮食,随后用谷实夜蛾幼虫侵袭。在每孔含有150μl人工昆虫饮食的浅96孔板中进行筛选。将~0.5μl表达MBP::Cry2Ax衍生多肽融合体的培养物掺入昆虫饮食。每孔放置约5个谷实夜蛾新生幼虫。昆虫测定板在29℃温育4天。昆虫对大肠杆菌样品的反应包括摄食抑制和死亡率。重新排列对幼虫引起严重发育迟缓或死亡的那些样品进行进一步分析。
所述第一轮文库筛选导致发现了杀虫活性相对于Cry2Ax和Cry2Ab*提高的几个克隆。具体是,发现克隆38(D_S00503480)(SEQID NO:4)和44(D_S00503970)(SEQ ID NO:6)当表达时活性很高(未显示数据)。因此选择这些克隆进行相继轮次的DNA改组。
对于第二轮改组,在尿嘧啶存在下,PCR扩增来自克隆38(D_S00503480)(SEQ ID NO:4)和44(D_S00503970)(SEQ ID NO:6)的亲本DNA模板,然后用尿嘧啶N-糖基化酶片段化。然后混合片段化的模板,在PCR扩增重组模板之前重新装配。在如上所述的pMAXY3219中生成这些改组模板的文库。从第一轮和第二轮改组分离的一些克隆的序列在表3中显示,其中指出了改变的氨基酸残基。
为了进一步多样化表现最好的第2轮命中物之一,克隆473R(D_S01037677)(SEQ ID NO:18),多肽氨基末端区的前46个氨基酸残基被修饰为含有在八个不同Cry2多肽序列(即Cry2Aa、Cry2Ab、Cry2Ac、Cry2Ad、Cry2Ae、Cry2Af、Cry2Ag和Cry2Ax)中发现的残基。此外,两个残基I13和D15分别被保守残基缬氨酸和谷氨酸取代(参见表4)。修饰的克隆称作克隆473N(SEQ ID NO:8)。
6.3实施例3:Cry2Ax衍生多肽的活性
在几个阶段进行改组的克隆活性的筛选。通过给一龄谷实夜蛾幼虫的人工饮食提供少量表达克隆融合蛋白的大肠杆菌,初步筛选克隆的高杀虫活性。选择引起幼虫全部死亡或全部发育迟缓的克隆进行进一步研究。然后,将显示高杀虫活性的那些克隆用于在植物表达载体,根癌土壤杆菌中生成新文库。通过每个克隆一式四份与本萨明那烟叶子共同培养(使用各自培养物每一种的强制渗入)来筛选文库,然后将每个相应盘喂给单独的3龄谷实夜蛾幼虫。24小时温育时间段后,通过目视观察测定摄食活力,并以剩余叶面积的近似分数表示。
将通过进一步重复大肠杆菌表达/饮食掺入测定的克隆再单独克隆入植物表达载体pMAXY4384并如上所述测试其在植物中(in planta)的功效。来自大肠杆菌表达多级测定和植物文库方法的最好命中物在植物中的最终活性估计在图1中显示。从这个分析看出,看来几个克隆具有增加的杀虫活性,包括7K(D_S01000779)(SEQ ID NO:10)、15K(D_S00999080)(SEQ ID NO:12)、16K(D_S01000269)(SEQ IDNO:14)、16R(D_S01037143)(SEQ ID NO:16)和473R(D_S01037677)(SEQ ID NO:18)。
6.4实施例4:DNA改组以分离另外的Cry2Ax衍生多肽
将如6.2节描述的第1轮和第2轮改组命中物的克隆44(D_S00503970)(SEQ ID NO:6)、473R(D_S01037677)(SEQ ID NO:18)和Cry2Ab*用作模板进行进一步改组。使用这些模板和寡核苷酸指导的改组,产生具有来自野生型Cry2多肽(即Cry2Ae和Cry2Ag)的氨基酸多样性以及在某些结构环区域片段内计算机产生的随机保守氨基酸取代和随机取代的衍生多肽。将改组的DNA文库通过取代Cry2Ab*基因克隆入pMAXY3219。建立毒素克隆,从而它们以与大肠杆菌麦芽糖结合蛋白(MBP)的融合体表达。分离的序列总结在表5-7中示出。
6.5实施例5:另外的Cry2Ax衍生多肽的活性
为了估计改组的衍生多肽抗棉花害虫谷实夜蛾的活性,如上描述进行使用含有表达克隆融合蛋白的完整大肠杆菌细胞的人工饮食的高通量筛选。进一步测试具有高水平活性的克隆在植物中的活性,以证实对每个衍生多肽作出的改变没有消极影响植物细胞中的基因表达或蛋白累积。为启动这个过程,将每个Cry2Ax衍生多肽克隆入基于根癌土壤杆菌的植物表达载体,转化入土壤杆菌菌株宿主,然后排列在微量滴定皿中。然后如下筛选命中物,通过将每个命中物一式四份与本萨明那烟叶共培养(使用各自培养物的每一种的强制渗入),随后将每个相应盘喂给单个三龄谷实夜蛾幼虫。24小时温育时间段后,通过如上所述的视觉俘获和分析方法测定每盘上的摄食活力。来自这个方法的一些衍生多肽与亲本克隆相比改善了。一个这种克隆是显示出活性比克隆44提高的D_S01764701(SEQ ID NO:134)。摄食测定的三次试验结果在图2显示。
6.6实施例6:表达克隆44的转基因植物
通过使用双元载体(binary vector)pMAXY5469和pMAXY5471,用草甘膦选择通过土壤杆菌介导的转化,产生表达克隆44(D_S00503970)的转基因烟草植物。这些载体含有dSVBV驱动的GAT基因和dMMV驱动的克隆44核酸分子克隆44(SEQ ID NO:5)。pMAXY5469不同于pMAXY5471,因为它含有与克隆44编码区融合的质体靶向信号,从而这个毒素变体将在质体室中累积。每个构建体产生大约25个转化体。表达克隆44的叶盘置于48孔滴定盘中的琼脂床上,然后用三龄谷实夜蛾幼虫或4龄甜菜夜蛾幼虫侵袭。使叶子与虫温育24小时,然后除去幼虫,并通过视频捕获设备观察剩余叶子,真实计算相对剩余叶面积(像素的数目)。每个载体使用最好转化体的结果对于谷实夜蛾在图3A中显示,而对于甜菜夜蛾,在图3B中显示。每个转基因植物具有如显示的选取用于进行分析的6个叶盘。
通过蛋白印迹测定克隆44多肽在转基因烟草植物质体(图4A)或在细胞质室(图4B)中的表达,其中使用针对克隆44多肽的多克隆抗体。图4中的泳道号码对应于图3中的植物号码。
蛋白印迹中使用的多克隆抗体通过如下方法制备,用纯化的胰蛋白酶截短的克隆44多肽免疫鸡,然后使用胰蛋白酶截短的克隆44多肽作为底物制备的亲和柱纯化Cry2特异性抗体。
两个转基因植物类型之间最明显的差异是甜菜夜蛾的抑制对于质体累积的毒素而言更大(比较图3B的右和左图)。这些数据结合表达数据(图4)表明含有来源于5469(图4A)的T-DNA的植物能够产生比5471(图4B)更多的毒素。
表1:Cry2Ax和Cry2Ax衍生序列
克隆名称 | 类型 | SEQID NO |
Cry2Ax | 核酸 | 1 |
Cry2Ax | 多肽 | 2 |
38(D_S00503480) | 核酸 | 3 |
38(D_S00503480) | 多肽 | 4 |
44(D_S00503970) | 核酸 | 5 |
44(D_S00503970) | 多肽 | 6 |
473N | 核酸 | 7 |
473N | 多肽 | 8 |
7K(D_S01000779) | 核酸 | 9 |
7K(D_S01000779) | 多肽 | 10 |
15K(D_S00999080) | 核酸 | 11 |
15K(D_S00999080) | 多肽 | 12 |
16K(D_S01000269) | 核酸 | 13 |
16K(D_S01000269) | 多肽 | 14 |
16R(D_S01037143) | 核酸 | 15 |
16R(D_S01037143) | 多肽 | 16 |
473R(D_S01037677) | 核酸 | 17 |
473R(D_S01037677) | 多肽 | 18 |
D_S01466681 | 核酸 | 19 |
D_S01466681 | 多肽 | 20 |
D_S01466770 | 核酸 | 21 |
D_S01466770 | 多肽 | 22 |
D_S01467219 | 核酸 | 23 |
D_S01467219 | 多肽 | 24 |
D_S01466712 | 核酸 | 25 |
D_S01466712 | 多肽 | 26 |
D_S01467003 | 核酸 | 27 |
D_S01467003 | 多肽 | 28 |
D_S01460229 | 核酸 | 29 |
D_S01460229 | 多肽 | 30 |
D_S01459398 | 核酸 | 31 |
D_S01459398 | 多肽 | 32 |
D_S01464856 | 核酸 | 33 |
D_S01464856 | 多肽 | 34 |
D_S014657862 | 核酸 | 35 |
D_S014657862 | 多肽 | 36 |
D_S01458733 | 核酸 | 37 |
D_S01458733 | 多肽 | 38 |
D_S01457892 | 核酸 | 39 |
D_S01457892 | 多肽 | 40 |
D_S01442158 | 核酸 | 41 |
D_S01442158 | 多肽 | 42 |
D_S01443366 | 核酸 | 43 |
D_S01443366 | 多肽 | 44 |
D_S01442132 | 核酸 | 45 |
D_S01442132 | 多肽 | 46 |
D_S01532970 | 核酸 | 47 |
D_S01532970 | 多肽 | 48 |
D_S01532041 | 核酸 | 49 |
D_S01532041 | 多肽 | 50 |
D_S01611723 | 核酸 | 51 |
克隆名称 | 类型 | SEQID NO |
D_S01611723 | 多肽 | 52 |
D_S01561293 | 核酸 | 53 |
D_S01561293 | 多肽 | 54 |
D_S01561489 | 核酸 | 55 |
D_S01561489 | 多肽 | 56 |
D_S01561330 | 核酸 | 57 |
D_S01561330 | 多肽 | 58 |
D_S01570511 | 核酸 | 59 |
D_S01570511 | 多肽 | 60 |
D_S01570809 | 核酸 | 61 |
D_S01570809 | 多肽 | 62 |
D_S01570568 | 核酸 | 63 |
D_S01570568 | 多肽 | 64 |
D_S01572168 | 核酸 | 65 |
D_S01572168 | 多肽 | 66 |
D_S01571315 | 核酸 | 67 |
D_S01571315 | 多肽 | 68 |
D_S01571875 | 核酸 | 69 |
D_S01571875 | 多肽 | 70 |
D_S01572374 | 核酸 | 71 |
D_S01572374 | 多肽 | 72 |
D_S01572905 | 核酸 | 73 |
D_S01572905 | 多肽 | 74 |
D_S01572908 | 核酸 | 75 |
D_S01572908 | 多肽 | 76 |
D_S01561856 | 核酸 | 77 |
D_S01561856 | 多肽 | 78 |
D_S01573294 | 核酸 | 79 |
D_S01573294 | 多肽 | 80 |
D_S01571529 | 核酸 | 81 |
D_S01571529 | 多肽 | 82 |
D_S01599948 | 核酸 | 83 |
D_S01599948 | 多肽 | 84 |
D_S01601459 | 核酸 | 85 |
D_S01601459 | 多肽 | 86 |
D_S01602925 | 核酸 | 87 |
D_S01602925 | 多肽 | 88 |
D_S01613034 | 核酸 | 89 |
D_S01613034 | 多肽 | 90 |
D_S01614407 | 核酸 | 91 |
D_S01614407 | 多肽 | 92 |
D_S01631557 | 核酸 | 93 |
D_S01631557 | 多肽 | 94 |
D_S01633080 | 核酸 | 95 |
D_S01633080 | 多肽 | 96 |
D_S01632237 | 核酸 | 97 |
D_S01632237 | 多肽 | 98 |
D_S01633031 | 核酸 | 99 |
D_S01633031 | 多肽 | 100 |
D_S01632121 | 核酸 | 101 |
D_S01632121 | 多肽 | 102 |
克隆名称 | 类型 | SEQID NO |
D_S01764500 | 核酸 | 103 |
D_S01764500 | 多肽 | 104 |
D_S01764502 | 核酸 | 105 |
D_S01764502 | 多肽 | 106 |
D_S01764505 | 核酸 | 107 |
D_S01764505 | 多肽 | 108 |
D_S01764533 | 核酸 | 109 |
D_S01764533 | 多肽 | 110 |
D_S01764543 | 核酸 | 111 |
D_S01764543 | 多肽 | 112 |
D_S01764546 | 核酸 | 113 |
D_S01764546 | 多肽 | 114 |
D_S01764554 | 核酸 | 115 |
D_S01764554 | 多肽 | 116 |
D_S01764568 | 核酸 | 117 |
D_S01764568 | 多肽 | 118 |
D_S01764569 | 核酸 | 119 |
D_S01764569 | 多肽 | 120 |
D_S01764577 | 核酸 | 121 |
D_S01764577 | 多肽 | 122 |
D_S01764642 | 核酸 | 123 |
D_S01764642 | 多肽 | 124 |
D_S01764643 | 核酸 | 125 |
D_S01764643 | 多肽 | 126 |
D_S01764680 | 核酸 | 127 |
D_S01764680 | 多肽 | 128 |
D_S01764685 | 核酸 | 129 |
D_S01764685 | 多肽 | 130 |
D_S01764691 | 核酸 | 131 |
D_S01764691 | 多肽 | 132 |
D_S01764701 | 核酸 | 133 |
D_S01764701 | 多肽 | 134 |
D_S01764706 | 核酸 | 135 |
D_S01764706 | 多肽 | 136 |
D_S01764723 | 核酸 | 137 |
D_S01764723 | 多肽 | 138 |
D_S02847715 | 核酸 | 139 |
D_S02847715 | 多肽 | 140 |
D_S01765051 | 核酸 | 141 |
D_S01765051 | 多肽 | 142 |
D_S01765068 | 核酸 | 143 |
D_S01765068 | 多肽 | 144 |
D_S01765100 | 核酸 | 145 |
D_S01765100 | 多肽 | 146 |
D_S01765063 | 核酸 | 147 |
D_S01765063 | 多肽 | 148 |
D_S01765119 | 核酸 | 149 |
D_S01765119 | 多肽 | 150 |
D_S01765104 | 核酸 | 151 |
D_S01765104 | 多肽 | 152 |
克隆名称 | 类型 | SEQID NO |
D_S01765112 | 核酸 | 153 |
D_S01765112 | 多肽 | 154 |
D_S01765174 | 核酸 | 155 |
D_S01765174 | 多肽 | 156 |
D_S01765242 | 核酸 | 157 |
D_S01765242 | 多肽 | 158 |
D_S01765308 | 核酸 | 159 |
D_S01765308 | 多肽 | 160 |
D_S01765221 | 核酸 | 161 |
D_S01765221 | 多肽 | 162 |
D_S01765254 | 核酸 | 163 |
D_S01765254 | 多肽 | 164 |
D_S01765231 | 核酸 | 165 |
D_S01765231 | 多肽 | 166 |
D_S01765255 | 核酸 | 167 |
D_S01765255 | 多肽 | 168 |
D_S01765377 | 核酸 | 169 |
D_S01765377 | 多肽 | 170 |
D_S01765430 | 核酸 | 171 |
D_S01765430 | 多肽 | 172 |
D_S01765446 | 核酸 | 173 |
D_S01765446 | 多肽 | 174 |
D_S01765496 | 核酸 | 175 |
D_S01765496 | 多肽 | 176 |
D_S01764642 | 核酸 | 177 |
D_S01764642 | 多肽 | 178 |
D_S01766041 | 核酸 | 179 |
D_S01766041 | 多肽 | 180 |
D_S01764706 | 核酸 | 181 |
D_S01764706 | 多肽 | 182 |
D_S01766073 | 核酸 | 183 |
D_S01766073 | 多肽 | 184 |
D_S01764643 | 核酸 | 185 |
D_S01764643 | 多肽 | 186 |
D_S01763985 | 核酸 | 187 |
D_S01763985 | 多肽 | 188 |
D_S01764668 | 核酸 | 189 |
D_S01764668 | 多肽 | 190 |
D_S01764196 | 核酸 | 191 |
D_S01764196 | 多肽 | 192 |
D_S01764728 | 核酸 | 193 |
D_S01764728 | 多肽 | 194 |
D_S01764787 | 核酸 | 195 |
D_S01764787 | 多肽 | 196 |
D_S01764758 | 核酸 | 197 |
D_S01764758 | 多肽 | 198 |
D_S01764768 | 核酸 | 199 |
D_S01764768 | 多肽 | 200 |
D_S01764860 | 核酸 | 201 |
D_S01764860 | 多肽 | 202 |
克隆名称 | 类型 | SEQID NO |
D_S01765018 | 核酸 | 203 |
D_S01765018 | 多肽 | 204 |
D_S01764947 | 核酸 | 205 |
D_S01764947 | 多肽 | 206 |
D_S01764934 | 核酸 | 207 |
D_S01764934 | 多肽 | 208 |
D_S01764968 | 核酸 | 209 |
D_S01764968 | 多肽 | 210 |
D_S01765008 | 核酸 | 211 |
D_S01765008 | 多肽 | 212 |
D_S01764953 | 核酸 | 213 |
D_S01764953 | 多肽 | 214 |
D_S01764977 | 核酸 | 215 |
D_S01764977 | 多肽 | 216 |
D_S01765509 | 核酸 | 217 |
D_S01765509 | 多肽 | 218 |
D_S01765668 | 核酸 | 219 |
D_S01765668 | 多肽 | 220 |
D_S01765621 | 核酸 | 221 |
D_S01765621 | 多肽 | 222 |
D_S01765693 | 核酸 | 223 |
D_S01765693 | 多肽 | 224 |
D_S01765687 | 核酸 | 225 |
D_S01765687 | 多肽 | 226 |
D_S01765765 | 核酸 | 227 |
D_S01765765 | 多肽 | 228 |
D_S01765932 | 核酸 | 229 |
D_S01765932 | 多肽 | 230 |
D_S01766010 | 核酸 | 231 |
D_S01766010 | 多肽 | 232 |
克隆名称 | 类型 | SEQID NO |
D_S01766026 | 核酸 | 233 |
D_S01766026 | 多肽 | 234 |
D_S02838294 | 核酸 | 235 |
D_S02838294 | 多肽 | 236 |
D_S02838310 | 核酸 | 237 |
D_S02838310 | 多肽 | 238 |
D_S02838327 | 核酸 | 239 |
D_S02838327 | 多肽 | 240 |
D_S02838328 | 核酸 | 241 |
D_S02838328 | 多肽 | 242 |
D_S02838330 | 核酸 | 243 |
D_S02838330 | 多肽 | 244 |
D_S02838454 | 核酸 | 245 |
D_S02838454 | 多肽 | 246 |
D_S02838470 | 核酸 | 247 |
D_S02838470 | 多肽 | 248 |
D_S02838478 | 核酸 | 249 |
D_S02838478 | 多肽 | 250 |
D_S02838434 | 核酸 | 251 |
D_S02838434 | 多肽 | 252 |
D_S02838549 | 核酸 | 253 |
D_S02838549 | 多肽 | 254 |
D_S02838632 | 核酸 | 255 |
D_S02838632 | 多肽 | 256 |
D_S02838640 | 核酸 | 257 |
D_S02838640 | 多肽 | 258 |
D_S02838648 | 核酸 | 259 |
D_S02838648 | 多肽 | 260 |
Cry2Ab | 多肽 | 261 |
表2:密码子表
表4:野生型Cry2多肽与克隆473N的序列比较
表5:使用Cry2Ab*作为亲本从DNA改组分离的目标克隆的序列
表6:使用克隆44作为亲本从DNA改组分离的目标克隆的氨基酸序列改变
序列表
<110>PIONEER HI-BRED INTERNATIONAL,INC.
<120>新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物
<130>V0337-PCT
<140>
<141>
<150>60/547,664
<151>2004-02-25
<160>261
<170>PatentIn Ver.3.3
<210>1
<211>1899
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>1
atgcataatg tattgaatag tggaagaact actatttgtg atgcgtataa tgtagtggct 60
catgatccat ttagttttca acataaatca ttagatacca tacaagaaga atggatggag 120
tggaaaaaag ataatcatag tttatatgta gatcctattg ttggaactgt ggctagtttt 180
cttttaaaga aagtggggag tcttgttgga aaaagaatac tgagtgagtt acggaattta 240
atatttccta gtggcagtac aaatctaatg caagatattt taagagagac agaaaaattc 300
ctgaatcaaa gacttaatac agacactctt gcccgtgtaa atgcggaatt gacagggctg 360
caagcaaatg tagaagagtt taatcgacaa gtagataatt ttttgaaccc taaccgaaat 420
gctgttcctt tatcaataac ttcttcagtt aatacaatgc agcaattatt tctaaataga 480
ttaacccagt tccagatgca aggataccaa ttgttattat tacctttatt tgcacaggca 540
gccaatttac atctttcttt tattagagat gttattctta atgcagacga atggggaatt 600
tcagcagcaa cattacgtac gtatcaaaat cacctgagaa attatacaag agattactct 660
aattattgta taaatacgta tcaaactgcg tttagaggtt taaacacccg tttacacgat 720
atgttagaat ttagaacata tatgttttta aatgtatttg agtatgtatc tatctggtcg 780
ttgtttaaat atcaaagcct tctagtctct tctggcgcta atttatatgc aagtggtagt 840
ggaccacagc agacccaatc atttacttca caagactggc catttttata ttctcttttc 900
caagttaatt caaattatgt gttaaatggc tttagtggcg ctagacttac gcagactttc 960
cctaatattg ttggtttacc tggtactact acaactcacg cattgcttgc tgcaagggtc 1020
aattacagtg gaggagtttc gtctggtgat ataggcgctg tgtttaatca aaattttagt 1080
tgtagcacat ttctcccacc tttgttaaca ccatttgtta ggagttggct agattcaggt 1140
tcagatcggg gggggatcaa taccgttacc aattggcaaa cagaatcctt tgagacaact 1200
ttaggtttaa ggagtggtgc ttttacagct cgaggtaatt caaactattt cccagattat 1260
tttatccgta atatttccgg agttccttta gttgttagaa atgaagattt aagaagaccg 1320
ttacactata atcaaataag aaatatagaa agtccttcag gaacacctgg tggattacga 1380
gcttatatgg tatctgtgca taacagaaaa aataatatct atgccgttca tgaaaatggt 1440
actatgattc atttagcgcc ggaagattat acaggattta ctatatcgcc gatacatgca 1500
actcaagtga ataatcaaac gcgaacattt atttctgaaa aatttggaaa tcaaggtgat 1560
tccttaagat ttgaacaaag caacacgaca gctcgttata cacttagagg gaatggaaat 1620
agttacaatc tttatttaag agtatcttca ataggaaatt ccactattcg agttactata 1680
aacggtagag tttatactgc ttcaaatgtt aatactacta caaataacga tggagttaat 1740
gataatggag ctcgtttttc agatattaat attggtaatg tagtagcaag tgataatact 1800
aatgtaccgt tagatataaa cgtgacatta aattctggta ctcaatttga gcttatgaat 1860
attatgtttg ttccaactaa tcttccacca ctttattaa 1899
<210>2
<211>632
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>2
Met His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala Tyr
1 5 10 15
Asn Val Val Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu Asp
20 25 30
Thr Ile Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser Leu
35 40 45
Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys Lys
50 55 60
Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn Leu
65 70 75 80
Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg Glu
85 90 95
Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala Arg
100 105 110
Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe Asn
115 120 125
Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro Leu
130 135 140
Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn Arg
145 150 155 160
Leu Thr Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro Leu
165 170 175
Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val Ile
180 185 190
Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr Tyr
195 200 205
Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys Ile
210 215 220
Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His Asp
225 230 235 240
Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr Val
245 250 255
Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser Gly
260 265 270
Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser Phe
275 280 285
Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn Ser
290 295 300
Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Thr Gln Thr Phe
305 310 315 320
Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu Leu
325 330 335
Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile Gly
340 345 350
Ala Val Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro Pro Leu
355 360 365
Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp Arg Gly
370 375 380
Gly Ile Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu Thr Thr
385 390 395 400
Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser Asn Tyr
405 410 415
Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu Val Val
420 425 430
Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile Arg Asn
435 440 445
Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr Met Val
450 455 460
Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu Asn Gly
465 470 475 480
Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr Ile Ser
485 490 495
Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe Ile Ser
500 505 510
Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Ser Asn
515 520 525
Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn Leu
530 535 540
Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val Thr Ile
545 550 555 560
Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr Asn Asn
565 570 575
Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn Ile Gly
580 585 590
Asn Val Val Ala Ser Asp Asn Thr Asn Val Pro Leu Asp Ile Asn Val
595 600 605
Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met Phe Val
610 615 620
Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>3
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>3
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaacac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtca gccttcagag gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt atcaagtggc gatattggag cttcgccgtt cacccagaac 1080
ttctcgtgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttgcaact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagcttca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgaattg 1860
atgaacatca tgttcgttcc gacaaaccta tcaccattgt at 1902
<210>4
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>4
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Thr Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Thr Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Ser Pro Leu Tyr
625 630
<210>5
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>5
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt at 1902
<210>6
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>6
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 450
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>7
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>7
atgggacaca acgtccttaa taacgaacgt aacacagtct gtgaagcaca caacgtggcc 60
gttcgcgacc ctttctcatt cgagcacaaa tcactaaaca ctgttgagaa ggagtggact 120
gagtggaaga gaaacgacca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccttgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat ccaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>8
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>8
Met Gly His Asn Val Leu Asn Asn Glu Arg Asn Thr Val Cys Glu Ala
1 5 10 15
His Asn Val Ala Val Arg Asp Pro Phe Ser Phe Glu His Lys Ser Leu
20 25 30
Asn Thr Val Glu Lys Glu Trp Thr Glu Trp Lys Arg Asn Asp His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Leu Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Ile Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>9
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>9
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgccaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtca gccttcagag gcctgaatac aaggctccac 720
gatgtgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt atcaagtggc gatattggag cttcgccgtt cacccagaac 1080
ttctcgtgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttgcaact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt at 1902
<210>10
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>10
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Val Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Thr Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>11
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>11
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gcattccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt cacccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagcttca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgaattg 1860
atgaacatca tgttcgttcc gacaaaccta tcaccattgt at 1902
<210>12
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>12
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Thr Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Ser Pro Leu Tyr
625 630
<210>13
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>13
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagcggaaga aggacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt cacccagaac 1080
ttctcgtgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagcttca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgaattg 1860
atgaacatca tgttcgttcc gacaaaccta tcaccattgt at 1902
<210>14
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>14
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Arg Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Thr Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Ser Pro Leu Tyr
625 630
<210>15
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>15
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtca gccttcagag gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt atcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttgcaact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagcttca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt at 1902
<210>16
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>16
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>17
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>17
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>18
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>18
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>19
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>19
atgggacatt ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagaa agaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgagttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>20
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>20
Met Gly His Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Lys Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>21
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>21
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgctcaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagt actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttagggacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcgc cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagct gggaaaccaa 1560
ggcgattctc tgaggtttga gcagacaaat accacggcaa ggtacacttt caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagccttg gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccaccaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatta tgctggtacc gacaaacatt agcccaatct attag 1905
<210>22
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>22
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asp Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Leu Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Thr Asn Thr Thr Ala Arg Tyr Thr Phe Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>23
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>23
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgcga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccgggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacgga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agatcttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccacgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgca cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>24
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>24
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val His Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>25
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>25
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggaaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacaccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagagga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcataac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagacaaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagccttg gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagccaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaccgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>26
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>26
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Thr Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Arg Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Thr Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Thr Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>27
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>27
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccctgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttctca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttagggatga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcgc cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccgaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acattcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat actacggcaa ggtacacttt caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagccttg gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcggt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>28
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>28
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Ser Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asp Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Phe Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Gly Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>29
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>29
atgggaaact ccgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctgcactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatatcggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgccact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gaccaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgggcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cctgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>30
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>30
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Ala Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Thr
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>31
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>31
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggtt 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctattcagga agagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaacac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gacccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt atcaagtggc gatattggag ctgttttcaa ccagaacttc 1080
tcgtgcagca cattcctgcc tcctttgctt acgccattcg ttagatcctg gctcgacagt 1140
ggaagtgatc gaggcggaat taatactgtg accaactggc agacagagag tttcgagaca 1200
acactcggtc tacgatcagg agcattcaca gcaagaggaa acagcaacta cttcccagac 1260
tacttcatcc gaaacatctc tggagtacct ctagtcgtta ggaacgaaga ccttcgtcgt 1320
cctctgcact acaatcaaat caggaacatt gaatcacctt caggtacacc tggtggactg 1380
cgagcataca tggtctcagt tcacaaccgt aagaacaaca tctacgcagt tcatgagaac 1440
ggaacgatga tccacttggc tccagaggac tacaccggat ttacaatcag tcctatccac 1500
gccactcagg tgaacaacca gactcgaacg ttcatcagtg agaagtttgg aaaccaaggc 1560
gattctctga ggtttgagca gtctaatacc acggcaaggt acactctcag gggtaatgga 1620
aactcttaca acctatactt gcgtgtctcc agcataggca attcaactat cagggttacc 1680
atcaacggtc gagtgtacac agcttctaac gtcaatacca ccactaacaa cgatggtgta 1740
aacgacaatg gtgctcgctt cagcgacatc aacatcggaa acgtagtcgc aagcgacaac 1800
accaacgtac ctctggacat taacgttacg ttgaactcag gcacacagtt cgaattgatg 1860
aacatcatgt tcgttccgac aaacctgccg ccattgtatt ag 1902
<210>32
<211>633
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>32
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Val Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Ile Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Thr Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Thr Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Val Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro Pro
355 360 365
Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp Arg
370 375 380
Gly Gly Ile Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu Thr
385 390 395 400
Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser Asn
405 410 415
Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu Val
420 425 430
Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile Arg
435 440 445
Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr Met
450 455 460
Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu Asn
465 470 475 480
Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr Ile
485 490 495
Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe Ile
500 505 510
Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Ser
515 520 525
Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn
530 535 540
Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val Thr
545 550 555 560
Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr Asn
565 570 575
Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn Ile
580 585 590
Gly Asn Val Val Ala Ser Asp Asn Thr Asn Val Pro Leu Asp Ile Asn
595 600 605
Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met Phe
610 615 620
Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>33
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>33
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>34
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>34
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>35
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>35
attggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag caccaacctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgtc cacggacacc ttggcacgag ttaatgctga attgaggggt 360
ctacaggcaa atgtcgagga gttcaatcac caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccta tgttccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc tccacctgag cttcatcagg gatgtcatcc taaacgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga aggagtacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctggac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtctcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cacaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>36
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>36
Ile Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Ser Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Arg Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn His Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Met Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Glu Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asp Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Leu Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Thr Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>37
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>37
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggtt 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctattcagga agagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaacac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gacccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt atcaagtggc gatattggag ctgttttcaa ccagaacttc 1080
tcgtgcagca cattcctgcc tcctttgctt acgccattcg ttagatcctg gctcgacagt 1140
ggaagtgatc gaggcggaat taatactgtg accaactggc agacagagag tttcgagaca 1200
acactcggtc tacgatcagg agcattcaca gcaagaggaa acagcaacta cttcccagac 1260
tacttcatcc gaaacatctc tggagtacct ctagtcgtta ggaacgaaga ccttcgtcgt 1320
cctctgcact acaatcaaat caggaacatt gaatcacctt caggtacacc tggtggactg 1380
cgagcataca tggtctcagt tcacaaccgt aagaacaaca tctacgcagt tcatgagaac 1440
ggaacgatga tccacttggc tccagaggac tacaccggat ttacaatcag tcctatccac 1500
gccactcagg tgaacaacca gactcgaacg ttcatcagtg agaagtttgg aaaccaaggc 1560
gattctctga ggtttgagca gtctaatacc acggcaaggt acactctcag gggtaatgga 1620
aactcttaca acctatactt gcgtgtctcc agcataggca attcaactat cagggttacc 1680
atcaacggtc gagtgtacac agcttctaac gtcaatacca ccactaacaa cgatggtgta 1740
aacgacaatg gtgctcgctt cagcgacatc aacatcggaa acgtagtcgc aagcgacaac 1800
accaacgtac ctctggacat taacgttacg ttgaactcag gcacacagtt cgaattgatg 1860
aacatcatgt tcgttccgac aaacctgccg ccattgtatt ag 1902
<210>38
<211>633
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>38
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Val Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Ile Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Thr Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Thr Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Val Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro Pro
355 360 365
Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp Arg
370 375 380
Gly Gly Ile Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu Thr
385 390 395 400
Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser Asn
405 410 415
Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu Val
420 425 430
Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile Arg
435 440 445
Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr Met
450 455 460
Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu Asn
465 470 475 480
Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr Ile
485 490 495
Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe Ile
500 505 510
Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Ser
515 520 525
Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn
530 535 540
Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val Thr
545 550 555 560
Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr Asn
565 570 575
Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn Ile
580 585 590
Gly Asn Val Val Ala Ser Asp Asn Thr Asn Val Pro Leu Asp Ile Asn
595 600 605
Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met Phe
610 615 620
Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>39
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>39
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata tgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac ggctacaccg gatttacaat cagtcccatc 1500
cacgccactc aggtgaacaa ccagactcga acattcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacacttt caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>40
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>40
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Gly Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Phe Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>41
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>41
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca tttgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcgggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacgggtcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattccc tgaggtttga gcaggcaaat accacggcaa ggtacacttt caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca acggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>42
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>42
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Leu
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg IIe Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ala Asn Thr Thr Ala Arg Tyr Thr Phe Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>43
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>43
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat gagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagacaaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagccttg gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagtgaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt ggcccattgt attag 1905
<210>44
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>44
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Met Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Thr Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Val Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Gly Pro Leu Tyr
625 630
<210>45
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>45
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattcgc tgaggtttga gcagacaaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagtgaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>46
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>46
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Thr Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Val Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>47
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>47
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcgcaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga gttgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gatttcctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact ctgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgcaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagccaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg ggaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>48
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>48
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln Arg Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Phe Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Leu Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>49
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>49
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatc ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgggga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattgggg cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta ccactagtcg ttaggaacga agacctccgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaaccctt acgacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtatacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaacatt agcccattgt attag 1905
<210>50
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>50
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Ser Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Gly Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Pro Tyr
530 535 540
Asp Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Tyr Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>51
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>51
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>52
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>52
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>53
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>53
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca ggaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcgtcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcatcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacgg 1020
gtcaactact ctggcggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacgttcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagcg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatgg gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>54
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>54
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Arg
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Val Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Met Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>55
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>55
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgcgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccgc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtttcaacac ctatcagacc gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaggtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacattg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>56
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>56
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Ala Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Phe Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Gly Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>57
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>57
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcagg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggcggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ccacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactcct acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtggt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc aacaaaccta ccaccattgt attag 1905
<210>58
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>58
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Arg Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn His Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>59
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>59
atgggacaca acgtccttaa ttccggtcgt accgcaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttgggac cgtggcttca 180
ttcttgctca agaaggtggg ctctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgaccc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcagttcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>60
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>60
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Ala Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Ser Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>61
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>61
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgcga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaga tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gcgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacagtca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacgaaccta ccaccattgt attag 1905
<210>62
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>62
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asp Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Ser Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>63
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>63
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcgtcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccggtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca tatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gctttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcgtag gcaattcaac tatcagggtt 1680
accatcaacg gccgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacccctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>64
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>64
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Val Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Arg Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn His Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Val Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>65
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>65
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa ccactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtc ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacggg ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca actttctaaa tccaactcaa 420
actgcaacgc gcttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagct tcatggtcgc caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcggtt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gccacctctg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatgct gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcagaaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>66
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>66
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Pro Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Thr Gln Thr Ala Thr Arg
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu His Gly Arg Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Ala Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>67
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>67
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactctgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaggt aggtggattt caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacctatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccgggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agcggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac actgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcccatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>68
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>68
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Cys
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gly Gly Phe Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Thr Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>69
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>69
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taagtgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccaaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gaccacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagggac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact cgggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>70
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>70
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Ser Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Gln Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp His Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Arg Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>71
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>71
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgaga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgactggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcagcac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtagc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>72
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>72
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Ser Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Ser Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>73
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>73
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca aaaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggc 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgccgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacgc gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcgggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt at 1902
<210>74
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>74
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Pro Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Ala Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>75
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>75
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgccatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt at 1902
<210>76
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>76
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Ala
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>77
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>77
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccttgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ccacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ccatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgactaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtatactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcgcaca gttcgatttg 1860
atgaacatca tgttcgttcc gacgaaccta ccaccattgt at 1902
<210>78
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>78
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Leu Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr His Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Ala Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>79
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>79
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gtcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ctctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgccga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaaccacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccgaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctagtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacgga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tgggaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta tcaccattgt at 1902
<210>80
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>80
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn His Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Ser Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Ser Pro Leu Tyr
625 630
<210>81
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>81
atgggacaca gcgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagacg ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgattttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaggt cgaaagatat caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccgacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt cgaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgattcactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc gggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcaagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt at 1902
<210>82
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>82
Met Gly His Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Ala Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Glu Arg Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asp Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asp Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Arg Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Gln Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>83
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>83
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtgg gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac gtacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggaccac agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaaaag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acgacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgttgt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgtcgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt at 1902
<210>84
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>84
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Gly Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Lys Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asp Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Ser Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>85
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>85
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attggaaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcgggtg ctaacctata cgcaagtgga 840
agtggaccac aacagagaca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaaac actacgactc atgccttgct ggctgcacgg 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt at 1902
<210>86
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>86
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Arg Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Asn Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>87
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>87
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggc 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gactacctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctaccagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ccaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcgagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt at 1902
<210>88
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>88
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>89
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>89
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttagaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggatac caactcttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg aactacctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggagtta ctaatacaca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactatt ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcgggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt at 1902
<210>90
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>90
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Arg Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn Tyr Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Val Thr Asn Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>91
<211>1902
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>91
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtgg gaccgagagg 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag tttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca attccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaagca tcgttggact tccaggaagc acgacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccggactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt at 1902
<210>92
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>92
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Gly Thr Glu Arg Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Ser Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Arg Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>93
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>93
atgggaaact ctgttcttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga aaaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggtta tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcac caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcggtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>94
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>94
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Val Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn His Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Arg Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>95
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>95
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgtcgtgc ctttgtctat tatgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg aactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>96
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>96
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Val Val Pro
130 135 140
Leu Ser Ile Met Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>97
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>97
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga tcctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggctacctc aattccagat gagacaatac caactcttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg aactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgagc ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>98
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>98
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Arg Gln Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Ser Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>99
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>99
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgtcgtgc ctttgtctat tatgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg aactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>100
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>100
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Val Val Pro
130 135 140
Leu Ser Ile Met Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>101
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>101
atgggaaact ctgtccttaa ctccggtcgt gccacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacggg ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cgattacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag gactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgcac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>102
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>102
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Ala Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu His Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>103
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>103
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaataga caagttgaca acttcctaaa tcccactcaa 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag ccctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cacaagtgga 840
agtggacctc agcaagccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcacag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>104
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>104
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Thr Gln Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Ala Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Thr Ser Gly Ser Gly Pro Gln Gln Ala Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Thr Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>105
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>105
atgggaaact ctgtccttaa ttccggtcgt accgcaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacgggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcaa 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacttggg cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctgcactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aagactccac 720
ggtacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgagc ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>106
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>106
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Ala Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Arg Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Gln Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Gly Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Ala Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Gly Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Ser Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>107
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>107
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggtt 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacagtcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa ccccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca accaaaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctggtcg ttaggaacga agaccttcgt 1320
cgtcctctgt acttcaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcccatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>108
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>108
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Val Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ser Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Gln Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu Tyr Phe Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>109
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>109
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccactaga 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cctcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacgctcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcgg acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>110
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>110
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Thr Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>111
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>111
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctcg agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcatgtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
tttcaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctagtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctagtacc gacaaacatt agcccattgt attag 1905
<210>112
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>112
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Glu
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Cys Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Ser Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>113
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>113
atgggaaact ccgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctgcactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatatcggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgccact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gaccaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgggcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cctgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>114
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>114
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Ala Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Thr
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>115
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>115
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagagg 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgccatcc taaatgcaga cgagtggggc 600
atatcggcag ccacactacg tacttatcag gactacctga agaactacac gcgtgactac 660
tcaaactact gtatcagcac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcgggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccgggaagc gctacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>116
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>116
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Arg Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Ala
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Ser Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Ala Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>117
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>117
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgtcggaac cgtggcttca 180
ttcttgctca agaaggtcgg ttctctcgtc ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcag gtatcgagga gttcaataga caagttgaca acttcctaaa tcccaatcgt 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcccatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac gtcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatcg tgctggtacc gacaaacatt agcccaatct attag 1905
<210>118
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>118
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Gly Ile Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His GIu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Val Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Val
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>119
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>119
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>120
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>120
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
5l5 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>121
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>121
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>122
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>122
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>123
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>123
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctcttgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaataga caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggaatac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac agcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc acgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct ctggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgacggt 1740
gtaaacgaca atggtgctcg tttcagcgac atcaaaatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc aacaaacatt agcccaatct attag 1905
<210>124
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>124
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Leu Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Glu Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Ser Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Trp Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr IIe Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Lys
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>125
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>125
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcaccagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctctggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacgctcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttgg gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>126
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>126
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Pro
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Gly Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn IIe Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>127
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>127
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc cttcctcatt ccagcacaaa tcactagact ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtcgg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggc 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaataga 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattcagaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccgc 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacca cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gcctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>128
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>128
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Ser Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Ser Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Arg Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu Arg
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn His Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>129
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>129
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tggaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcgaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccgggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacgg 1020
gtcaactact ctggcggaat ctcaagtggc gatattggag cttcaccatt cagccagaac 1080
tttaactgca gcacattcac ccctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggacgtg accgagaagg tgtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gaccacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaactat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cctgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacaaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>130
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>130
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Trp Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Arg Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Arg Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Ser Gln Asn Phe Asn Cys Ser Thr Phe Thr Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Arg Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp His Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Tyr Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asn Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>131
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>131
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>132
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>132
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>133
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>133
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgtcggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgga cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
ggtacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccctgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca ttcgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacacca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>134
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>134
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asp Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Gly Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val SerIle Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp IIe Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Thr Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>135
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>135
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>136
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>136
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>137
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>137
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttcttatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggggc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga aatgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcaa 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacgt tgcagcagct attcttgaac 480
cggttacctc aattcagagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcgtcc taaatgcgga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaagtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgcccgatc gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaagaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>138
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>138
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Leu Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Met Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Gln Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Leu Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Arg Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser PheIle Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Ser Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Ser Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Lys Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>139
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>139
atggggaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata cgacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcatt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcgcgtg ttaatgctga attggaaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tccccctaga 420
agggccgtgc acttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gcggctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcagg cgagtggggc 600
atatcggcag ctacactacg tacttatcag gactacctga agaactacac gcgtgactac 660
tcaaactact gcatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actatgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cctcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>140
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>140
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asp Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Pro Arg Arg Ala Val His
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Gly Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Met Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Leu Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>141
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>141
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata taacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
gggaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>142
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>142
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>143
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>143
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgaattg 1860
atgaacatca tgttcgttcc gacaaaccta tcaccattgt attag 1905
<210>144
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>144
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Ser Pro Leu Tyr
625 630
<210>145
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>145
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata taacgtggcc 60
gctcacgacc ctttttcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
gggaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>146
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>146
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>147
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>147
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgtcggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgga cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
ggtacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccctgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca ttcgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacacca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>148
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>148
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asp Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Gly Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg AsnIle Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Thr Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>149
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>149
atgggaaact ctgtccttaa ttccggtcgc accacaatat gcgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ttttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacgga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>150
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>150
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Leu
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly AspIle
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>151
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>151
atgggaaact ctgtccttaa ttccggtcat accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctcttgtt ggtaagagga ttctctcgga actaaggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccatga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc tgggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggtcacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gaagtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcagaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
tcaacaattg gtattcgatc cagagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac ggctacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ctagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>152
<211>633
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>152
Met Gly Asn Ser Val Leu Asn Ser Gly His Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu His
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Trp Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Ser Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Glu Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Ser Thr Ile Gly Ile Arg Ser Arg Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Gly Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Thr Arg Thr Phe Ile
500 505 510
Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Asn
515 520 525
Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn
530 535 540
Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val Thr
545 550 555 560
Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr Asn
565 570 575
Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn Met
580 585 590
Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile Asn
595 600 605
Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met Leu
610 615 620
Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>153
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>153
atgggaaact ctgtccttaa ttccggtcgt accacaacac gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat gttgacccgg tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacagatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgc 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacgcga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac cagttgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacttgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgctgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ctcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca acggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>154
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>154
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Thr Arg Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Val Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asp Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Ala Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Leu Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>155
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>155
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>156
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>156
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>157
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>157
atgggacaca acgtccttaa ttccggtcgc accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcagggta tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagct gaataatcgt caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccaaact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg gtcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggtgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>158
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>158
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Gly Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu Asn Asn Arg Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Gly
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn AsnIle Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>159
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>159
atgggaaact ctgtccttaa ttccggtcgc accacaatat gcgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ttttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacgga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>160
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>160
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Leu
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>161
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>161
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca ggaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgttggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt cgaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt agctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttgg gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccgttgt attag 1905
<210>162
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>162
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Arg
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asp Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Gly Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>163
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>163
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>164
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>164
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>165
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>165
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgccga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaagca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cctcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcgtgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgacttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>166
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>166
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Ser Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val Arg Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>167
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>167
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgacgcata caacgtggca 60
gctcacgacc ctttctcatt ccggcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttcg 180
ttcttgctca agaaggtcgg ttctctcgtc ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcaa caagttgaca acttcctaaa tcccaatcgc 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gacacgttgg agtttcggac atacatgttc ctgaacgtgt tcgaatatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ctcaagcggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>168
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>168
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Arg His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Gln Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>169
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>169
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcaccagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctctggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacgctcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttgg gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>170
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>170
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Pro
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Gly Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>171
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>171
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>172
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>172
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>173
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>173
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata taacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
gggaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>174
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>174
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>175
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>175
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccggcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcatc ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcga caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca attgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>176
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>176
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Arg His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Leu
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>177
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>177
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctcttgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaataga caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggaatac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac agcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc acgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct ctggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgacggt 1740
gtaaacgaca atggtgctcg tttcagcgac atcaaaatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc aacaaacatt agcccaatct attag 1905
<210>178
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>178
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Leu Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser PheIle Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Glu Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Ser Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr ValThr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Trp Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Lys
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>179
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>179
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttcttatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaagagt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacggtc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccaccc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgtaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>180
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>180
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Leu Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Lys Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Val Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr AsnIle Ser Pro Ile Tyr
625 630
<210>181
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>181
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>182
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>182
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>183
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>183
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaagccca atcgttcacg agtcaggact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ccggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
tttaactgca gcacattcac ccctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc accgcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>184
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>184
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Ala Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Thr Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
4654 70 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>185
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>185
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcaccagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctctggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacgctcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttgg gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>186
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>186
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Pro
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Gly Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>187
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>187
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaaccacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata tgcaagtgga 840
agtggagtta ctaatacaca atcgttcacg agtcaagact ggccattcct gtacagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>188
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>188
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn His Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Val Thr Asn Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>189
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>189
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacgggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaataga 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggaatac 660
tcaaactact gtatcaacac ctaccagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactacc ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
tcaacaattg gtattcgatc cggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>190
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>190
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Arg Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Glu Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Pro Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Ser Thr Ile Gly Ile Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>191
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>191
atgggaaact ctgtccttaa ttccagtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcagggta tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gagaggatac caatctttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gacgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag gactacctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac agggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacagcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagcgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>192
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>192
Met Gly Asn Ser Val Leu Asn Ser Ser Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Gly Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Arg Gly Tyr Gln Ser Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Gly Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Ala Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>193
<211>1906
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>193
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactggaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat attgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cgcggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacatga tgcagcagct attcttgaac 480
cggttacctc aattccagct acgtgatccc caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaacg tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaacg acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gccgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt gcgttgaact caggcacaca gttcgatttg 1860
atgaacgtca tgtttgttcc gacaaaccta ccaccattgt attagg 1906
<210>194
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>194
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Ile Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Ala Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Met Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu Arg Asp Pro Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Val Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asp Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Ala Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Val Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>195
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>195
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctata tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatgc aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>196
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>196
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Tyr Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Ala Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>197
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>197
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaacca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagtt aggtgaatat caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccatccct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgttcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cccgaacagc cggccagaac 1080
ttcaactgca gcacattcct gcctcctttg ctttcgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ctaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcataca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>198
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>198
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu Gly Glu Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Ser Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Val Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Arg Thr Ala Gly Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Ser Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Ala Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Ile Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>199
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>199
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>200
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>200
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>201
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>201
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>202
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>202
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>203
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>203
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata taacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
gggaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>204
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>204
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>205
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>205
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttcttatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaagagt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacggtc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccaccc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgtaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>206
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>206
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Leu Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Lys Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Val Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>207
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>207
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctata tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatgc aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>208
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>208
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Tyr Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Ala Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>209
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>209
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag aatcacctgc gcaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgcgtcac cttcaggtac acctggtgga 1380
ttgcgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggatcca tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagc 1800
aacagcgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgttcgttcc gacaaaccta ccaccattgt attag 1905
<210>210
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>210
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>211
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>211
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggaca tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgcagtgc ctttgtctat tacgtcgtcc gttaacacga tgcagcagct attcttgagc 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
tcaacaattg gtattcgatg cggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct cgggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>212
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>212
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Ser
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Ser Thr Ile Gly Ile Arg Cys Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr PheIle Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Gly Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>213
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>213
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcaca caacgtggcc 60
gctcacgacc ctttctcatt ccagcgcaaa tcactagaca ctgttcagga ggggtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacgggt 360
ctacaggcaa gtgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctgt tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagtt gcgtggacac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcgagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggcgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gccgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>214
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>214
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
His Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln Arg Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Gly Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Ser Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Val Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu Arg Gly His Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Ala Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>215
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>215
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcgctagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttactca agaaggtcgg ttctctcgtc ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttga agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacccc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgtcagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctggtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg ggtttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaaccctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgggtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>216
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>216
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Lys Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Pro Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>217
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>217
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcgta caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattcagagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacgctcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtggg 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cgcagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>218
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>218
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Arg Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Ala Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>219
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>219
atgggacaca acgtccttaa ttccggtcgc accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcagggta tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagct gaataatcgt caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccaaact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg gtcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggtgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>220
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>220
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Gly Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Leu Asn Asn Arg Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Gly
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>221
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>221
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgga cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
gtatcggcag ctacactacg tacttatagg gattacctga agaactacac gacggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattgggg cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacggtc cggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cggcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctgtc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac catcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>222
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>222
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asp Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Val Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Thr Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Val His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>223
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>223
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgccga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaagca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattggag cctcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcgtgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgacttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>224
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>224
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Ser Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val Arg Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>225
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>225
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>226
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>226
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>227
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>227
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc cttcctcatt ccagcacaaa tcactagact ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtcgg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggc 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaataga 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattcagaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccgc 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacca cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gcctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>228
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>228
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Ser Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Ser Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Arg Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu Arg
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn His Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>229
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>229
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctata tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatgc aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>230
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>230
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Tyr Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Ala Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>231
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>231
atgggacaca acgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggcc 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagga ggagtggatg 120
gagtggaaga aggacaatca ttcgctatat gttgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggagc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tccgcaccca 420
actatctctc acttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttaccac aattccagat gcaaggctac caattgttgt tactcccggt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcga aaccatctga ggaactacac gcgtgactac 660
tcaaaccact gtatcaacac ctatcagact gccttccgtg gcctgaatac aaggctccac 720
gatatgttgg agtttcggac atacatgtcc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccagcta cgtgctgaac ggcttctcag gtgctcgatt gtcccagact 960
ttcccaaaca tcgttggact tccaggaaca actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggagt ttcaagtggc gatattgggg cttcgccgtt caaccagaac 1080
ttcagttgca gcacattcct gcctcctttg cttacgccat tcgttagatc ccggctcgac 1140
agtggaagtg atcgaggggg agttaatact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatca gatcaggaac attgagtcac ctccaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatctacgc agttcatgag 1440
aacggaacga tgatccactt ggctccagag gactacaccg gatttacaat cagtcctatc 1500
cacgccactc gggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagagtaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtc 1680
accatcaacg gtcgagtgta cacagctgca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcgagcgac 1800
aacagcaacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgtttgttcc gacaaaccta ccaccattgt attag 1905
<210>232
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>232
Met Gly His Asn Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Glu Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro His Pro Thr Ile Ser His
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Val Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn His Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Ser Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Ser Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Gln Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Arg Leu Asp Ser Gly Ser Asp
370 375 380
Arg Gly Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Gln Ile
435 440 445
Arg Asn Ile Glu Ser Pro Pro Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu
465 470 475 480
Asn Gly Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Arg Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Ser Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Ala Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Asp Asn Ser Asn Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Phe Val Pro Thr Asn Leu Pro Pro Leu Tyr
625 630
<210>233
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>233
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacgatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgaggag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcaa 420
aacgcagtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgctagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcgacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>234
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>234
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asp His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Glu Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Gln Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asp
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>235
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>235
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctata tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatgc aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>236
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>236
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Tyr Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Ala Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>237
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>237
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag gactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cggtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cgcagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>238
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>238
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Gly Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Ala Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>239
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>239
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgaggag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaacttg 420
gacaccgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caactgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaggact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>240
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>240
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Glu Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Leu Asp Thr Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>241
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>241
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgcga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaata tgcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccgggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacgga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agatcttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccacgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgca cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>242
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>242
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys ArgIle Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln AspIle Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val His Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>243
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>243
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actatggaat 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctata tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaagt gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc tgaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatgc aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cccctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggct 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatgg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttcaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>244
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>244
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Trp Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Tyr Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Ala Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Ala
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Met Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Phe Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>245
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>245
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggggt 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgga cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccaaat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcgtcc taaatgcaga cgagtggggc 600
gtatcggcag ctacactacg tacttatagg gattacctga agaactacac gacggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcggtggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattgggg cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacggtc cggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cggcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctgtc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac catcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>246
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>246
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Gly
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asp Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Val Leu Asn Ala Asp Glu Trp Gly Val Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Thr Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Val His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>247
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>247
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagcaa 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacaccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gagggactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcgaac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gcatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacccctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccaatct attag 1905
<210>248
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>248
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Thr Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu His Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Ile Tyr
625 630
<210>249
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>249
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>250
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>250
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>251
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>251
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caggttgaca acctcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt actcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gactacctga ggaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacccc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>252
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>252
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Leu Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Leu Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>253
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>253
atgggaaact ctgtccttaa ctccggtcgt gccacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatcag gactacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cggtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cgcagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>254
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>254
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Ala Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Gln Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Gly Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Ala Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>255
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>255
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcattagaca ctgttcggag ggaatggact 120
gagtggaaga agaacaatca ttcgccatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggaac 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attgacaggc 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tccccctttg 420
agcattgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacctc aattccagat gcaacaatac caactcttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctaccagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc actgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>256
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>256
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Arg Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Pro Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Pro Leu Ser Ile Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gln Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>257
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>257
atgggaaact ctgtccttaa ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa tcactagaca ctgttcagag ggaatggact 120
gagtggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggaca tactccgtga gaccgagaag 300
ttcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attggaaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tccccctaga 420
gacattgtgc ccttgtctat tacgtcgtcc gtcaacacga tgcaacagct attcttgaac 480
cggttacctc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcaga cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagaca gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ccaacctata cgcaagcgga 840
agtggacctc agcaaaccca atcgttcacg ggtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgtcggact tccaggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcgtca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caggggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>258
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>258
Met Gly Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Pro Arg Asp Ile Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Thr Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Gly Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Val Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>259
<211>1905
<212>DNA
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>259
atgggaaact ctgtccttga ttccggtcgt accacaatat gtgatgcata caacgtggca 60
gctcacgacc ctttctcatt ccagcacaaa ccactagaca ctgttcagag ggaatggact 120
gagcggaaga agaacaatca ttcgctatat ctcgacccga tcgttggaac cgtggcttca 180
ttcttgctca agaaggtggg ttctctcgtt ggtaagagga ttctctcgga actaaggtcc 240
ttgatcttcc cctccggtag cacaaatctc atgcaggata tactccgtga gaccgagaag 300
ctcctgaacc agcgactgaa cacggacacc ttggcacgag ttaatgctga attggaaggt 360
ctacaggcaa atgtcgagga gttcaatcgc caagttgaca acttcctaaa tcccaatcgt 420
aacgccgtgc ctttgtctat tacgtcgtcc gtcaacacga tgcagcagct attcttgaac 480
cggttacccc aattccagat gcaaggctac caattgttgt tactcccgtt attcgcccaa 540
gctgctaatc ttcacctgag cttcatcagg gatgtcatcc taaatgcagg cgagtggggc 600
atatcggcag ctacactacg tacttatagg gattacctga agaactacac gcgtgactac 660
tcaaactact gtatcaacac ctatcagtcc gccttcaaag gcctgaatac aaggctccac 720
gatacgttgg agtttcggac atacatgttc ctgaacgtgt tcgagtatgt ctccatctgg 780
tcgcttttca agtaccagtc attgctggtc tcgtcaggtg ctaacctata cgcaagtgga 840
agtggacctc agcaaaccca atcgttcacg agtcaagact ggccattcct gtatagcttg 900
ttccaggtca actccaacta cgtgctgaac ggcttctcag gtgctcgatt gtccaacact 960
ttcccaaaca tcgttggact tccgggaagc actacgactc atgccttgct ggctgcacga 1020
gtcaactact ctggtggaat ctcaagtggc gatattggag cttcaccatt caaccagaac 1080
ttcaactgca gcacattcct gcctcctttg cttacgccat tcgttagatc ctggctcgac 1140
agtggaagtg atcgagaagg agtcgctact gtgaccaact ggcagacaga gagtttcgag 1200
acaacactcg gtctacgatc aggagcattc acagcaagag gaaacagcaa ctacttccca 1260
gactacttca tccgaaacat ctctggagta cctctagtcg ttaggaacga agaccttcgt 1320
cgtcctctgc actacaatga gatcaggaac attgcctcac cttcaggtac acctggtgga 1380
gcacgagcat acatggtctc agttcacaac cgtaagaaca acatccatgc agttcatgag 1440
aacggatcaa tgatccactt ggctccaaac gactacaccg gatttacaat cagtcctatc 1500
cacgccactc aggtgaacaa ccagactcga acgttcatca gtgagaagtt tggaaaccaa 1560
ggcgattctc tgaggtttga gcagaacaat accacggcaa ggtacactct caagggtaat 1620
ggaaactctt acaacctata cttgcgtgtc tccagcatag gcaattcaac tatcagggtt 1680
accatcaacg gtcgagtgta cacagctaca aacgtcaata ccaccactaa caacgatggt 1740
gtaaacgaca atggtgctcg cttcagcgac atcaacatcg gaaacgtagt cgcaagcagt 1800
aacagtgacg tacctctgga cattaacgtt acgttgaact caggcacaca gttcgatttg 1860
atgaacatca tgctggtacc gacaaacatt agcccattgt attag 1905
<210>260
<211>634
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>260
Met Gly Asn Ser Val Leu Asp Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Pro Leu
20 25 30
Asp Thr Val Gln Arg Glu Trp Thr Glu Arg Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Ser
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Leu Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Glu Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Gly Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Thr Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Lys Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>261
<211>632
<212>PRT
<213>苏云金芽孢杆菌(Bacillus thuringiensis)
<400>261
Met Asn Asn Val Leu Asn Asn Gly Arg Thr Thr Ile Cys Asp Ala Tyr
1 5 10 15
Asn Val Val Ala His Asp Pro Phe Ser Phe Glu His Lys Ser Leu Asp
20 25 30
Thr Ile Arg Lys Glu Trp Met Glu Trp Lys Arg Thr Asp His Ser Leu
35 40 45
Tyr Val Ala Pro Ile Val Gly Thr Val Ser Ser Phe Leu Leu Lys Lys
50 55 60
Val Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser Glu Leu Trp Gly Leu
65 70 75 80
Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg Glu
85 90 95
Thr Glu Gln Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala Arg
100 105 110
Val Asn Ala Glu Leu Glu Gly Leu Gln Ala Asn Ile Arg Glu Phe Asn
115 120 125
Gln Gln Val Asp Asn Phe Leu Asn Pro Thr Gln Asn Pro Val Pro Leu
130 135 140
Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn Arg
145 150 155 160
Leu Pro Gln Phe Arg Val Gln Gly Tyr Gln Leu Leu Leu Leu Pro Leu
165 170 175
Phe Ala Gln Ala Ala Asn Met His Leu Ser Phe Ile Arg Asp Val Val
180 185 190
Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr Tyr
195 200 205
Gln Asn Tyr Leu Lys Asn Tyr Thr Thr Glu Tyr Ser Asn Tyr Cys Ile
210 215 220
Asn Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His Asp
225 230 235 240
Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr Val
245 250 255
Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser Gly
260 265 270
Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser Phe
275 280 285
Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn Ser
290 295 300
Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Thr Gln Thr Phe
305 310 315 320
Pro Asn Ile Gly Gly Leu Pro Gly Thr Thr Thr Thr His Ala Leu Leu
325 330 335
Ala Ala Arg Val Asn Tyr Ser Gly Gly Val Ser Ser Gly Asp Ile Gly
340 345 350
Ala Val Phe Asn Gln Asn Phe Ser Cys Ser Thr Phe Leu Pro Pro Leu
355 360 365
Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp Arg Gly
370 375 380
Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu Ser Thr
385 390 395 400
Leu Gly Leu Arg Cys Gly Ala Phe Thr Ala Arg Gly Asn Ser Asn Tyr
405 410 415
Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu Val Val
420 425 430
Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile Arg Asn
435 440 445
Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Leu Arg Ala Tyr Met Val
450 455 460
Ser Val His Asn Arg Lys Asn Asn Ile Tyr Ala Val His Glu Asn Gly
465 470 475 480
Thr Met Ile His Leu Ala Pro Glu Asp Tyr Thr Gly Phe Thr Ile Ser
485 490 495
Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe Ile Ser
500 505 510
Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Ser Asn
515 520 525
Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn Leu
530 535 540
Tyr Leu Arg Val Ser Ser Leu Gly Asn Ser Thr Ile Arg Val Thr Ile
545 550 555 560
Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr Asn Asn
565 570 575
Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Leu Asp Ile Asn Met Gly
580 585 590
Asn Val Val Ala Ser Asp Asn Thr Asn Val Pro Leu Asp Ile Asn Val
595 600 605
Thr Phe Asn Ser Gly Thr Gln Phe Glu Leu Met Asn Ile Met Phe Val
610 615 620
Pro Thr Asn Leu Pro Pro Ile Tyr
625 630
Claims (20)
1.由SEQ ID NO:133或其互补物组成的分离的核酸分子。
2.选自下组的分离的核酸分子:
a)编码由SEQ ID NO:134的氨基酸序列组成的多肽的核酸分子;和
b)在0.2X SSC、55℃洗涤至少一次,洗涤20分钟后,与由SEQ ID NO:133或其互补物的核苷酸序列组成的核酸探针杂交且具有至少一种杀虫活性的核酸分子。
3.权利要求2的分离的核酸分子,其中所述核酸分子编码包含选自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少一个氨基酸的多肽。
4.权利要求1或2的分离的核酸分子,其中所述核酸分子编码具有杀虫活性的多肽。
5.权利要求4的分离的核酸分子,其中双翅目或鳞翅目对所述多肽的杀虫活性易感。
6.包含权利要求1或2的核酸分子的载体。
7.权利要求6的载体,它是表达载体。
8.包含权利要求6的载体的宿主细胞。
9.权利要求8的宿主细胞,其中所述细胞选自植物细胞、细菌细胞、真菌细胞。
10.由SEQ ID NO:134组成的分离的多肽。
11.由核酸分子编码的分离的多肽,该核酸分子在0.2X SSC、55℃洗涤至少一次,洗涤20分钟后,与由SEQ ID NO:133或其互补物的核苷酸序列组成的核酸探针杂交且具有至少一种杀虫活性。
12.权利要求11的分离的多肽,其中所述多肽包含选自H2、S7、Q27、Q35、E36、K43、D44、N45、D51、A58、V69、R78、N79、K99、T118、V124、E125、R129、N138、R139、A141、T162、Q165、M166、L183、I192、H211、R213、R217、D218、V324、I386、T399、S405、Q445、I551、S587、I591、L610和L631的至少一个氨基酸。
13.权利要求10或11的分离的多肽,其中所述多肽具有杀虫活性。
14.权利要求13的分离的多肽,其中双翅目或鳞翅目对所述多肽的杀虫活性易感。
15.包含SEQ ID NO:134的多肽和试剂的组合物,其中该试剂选自涂布器粘着剂佐剂、稳定剂、杀虫剂、稀释剂、表面活性剂、乳化剂和分散剂。
16.权利要求15的组合物,其中所述组合物进一步包含细胞。
17.权利要求16的组合物,其中所述细胞是完整的或溶解的。
18.权利要求17的组合物,其中所述细胞处于悬浮液中或是急降的。
19.权利要求15的组合物,其中所述组合物进一步包含苏云金芽孢杆菌的芽孢晶体复合物。
20.权利要求15的组合物,其中所述多肽是纯化的。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US54766404P | 2004-02-25 | 2004-02-25 | |
US60/547,664 | 2004-02-25 | ||
US60/547664 | 2004-02-25 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA2005800117014A Division CN1942582A (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101671681A true CN101671681A (zh) | 2010-03-17 |
CN101671681B CN101671681B (zh) | 2012-11-28 |
Family
ID=34910926
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101635114A Active CN101671680B (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
CNA2005800117014A Pending CN1942582A (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
CN2009101673582A Active CN101671681B (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101635114A Active CN101671680B (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
CNA2005800117014A Pending CN1942582A (zh) | 2004-02-25 | 2005-02-25 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
Country Status (16)
Country | Link |
---|---|
US (7) | US7208474B2 (zh) |
EP (2) | EP2184360B1 (zh) |
JP (2) | JP4818252B2 (zh) |
KR (2) | KR20120035233A (zh) |
CN (3) | CN101671680B (zh) |
AR (1) | AR048744A1 (zh) |
AU (2) | AU2005216528B2 (zh) |
BR (1) | BRPI0508068B1 (zh) |
CA (2) | CA2556736C (zh) |
CL (1) | CL2009001136A1 (zh) |
ES (1) | ES2552053T3 (zh) |
HU (1) | HUE028143T2 (zh) |
MX (1) | MXPA06009694A (zh) |
PT (1) | PT2184360E (zh) |
WO (1) | WO2005082077A2 (zh) |
ZA (1) | ZA200607222B (zh) |
Families Citing this family (59)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101238215A (zh) * | 2005-04-05 | 2008-08-06 | 先锋高级育种国际公司 | 利用植物病毒密码子偏爱性设计用于在植物中的多肽表达的核酸分子的方法和组合物 |
EP3181579A1 (en) * | 2007-10-16 | 2017-06-21 | Athenix Corporation | Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use |
US8110608B2 (en) | 2008-06-05 | 2012-02-07 | Ecolab Usa Inc. | Solid form sodium lauryl sulfate (SLS) pesticide composition |
US8729336B2 (en) * | 2009-01-23 | 2014-05-20 | Pioneer Hi-Bred International, Inc | Protein mixtures for maize insect control |
JP2012529269A (ja) * | 2009-06-05 | 2012-11-22 | ジ オハイオ ステイト ユニヴァーシティ リサーチ ファウンデーション | 生体材料、組成物及び方法 |
BR112012031231A2 (pt) | 2010-06-09 | 2017-08-29 | Du Pont | Construto de dna recombinante, planta, semente, método para identificar um íntron e método para modular a expressão de transgene em um planta monocotiledônea |
AU2011289283B2 (en) | 2010-08-13 | 2015-04-30 | Pioneer Hi-Bred International, Inc. | Chimeric promoters and methods of use |
US8968757B2 (en) | 2010-10-12 | 2015-03-03 | Ecolab Usa Inc. | Highly wettable, water dispersible, granules including two pesticides |
US9204603B2 (en) | 2011-12-21 | 2015-12-08 | The Curators Of The University Of Missouri | Soybean variety S05-11482 |
US20130167262A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
CN112400900A (zh) | 2012-03-09 | 2021-02-26 | 韦斯塔隆公司 | 毒肽产生、植物中的肽表达和富含半胱氨酸的肽的组合 |
US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
WO2013166113A1 (en) | 2012-05-04 | 2013-11-07 | E. I. Du Pont De Nemours And Company | Compositions and methods comprising sequences having meganuclease activity |
BR112015017788A2 (pt) * | 2013-01-25 | 2017-08-22 | Pioneer Hi Bred Int | Construto de dna, método para produzir uma planta, método de detecção da presença de uma molécula de ácido nucleico, par de iniciadores de polinucleotídio, evento de milho, método de detecção da presença do dna e kit para detectar ácidos nucleicos |
WO2014116854A1 (en) * | 2013-01-25 | 2014-07-31 | Pioneer Hi-Bred International, Inc. | Maize event dp-033121-3 and methods for detection thereof |
WO2014116989A1 (en) * | 2013-01-25 | 2014-07-31 | Pioneer Hi-Bred International, Inc. | Maize event dp-032218-9 and methods for detection thereof |
EA028528B1 (ru) | 2013-03-13 | 2017-11-30 | Пайонир Хай-Бред Интернэшнл, Инк. | Внесение глифосата для борьбы с сорняками у brassica |
US20140289906A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
CN115960896A (zh) | 2013-03-14 | 2023-04-14 | 先锋国际良种公司 | 用以防治昆虫害虫的组合物和方法 |
AU2014236162A1 (en) | 2013-03-14 | 2015-09-17 | Arzeda Corp. | Compositions having dicamba decarboxylase activity and methods of use |
WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
EA030896B1 (ru) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
BR122020001770B1 (pt) | 2013-09-13 | 2022-11-29 | Pioneer Hi-Bred International, Inc | Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto |
US10227608B2 (en) | 2014-02-07 | 2019-03-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
CN106232620B (zh) | 2014-02-07 | 2022-05-13 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CA2961733A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
US10435706B2 (en) | 2014-10-16 | 2019-10-08 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2016099916A1 (en) | 2014-12-19 | 2016-06-23 | E. I. Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
EP3262062A1 (en) | 2015-02-04 | 2018-01-03 | Pioneer Hi-Bred International, Inc. | Novel bt toxin receptors and methods of use |
CN108064233B (zh) | 2015-05-19 | 2022-07-15 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
CA2986265A1 (en) | 2015-06-16 | 2016-12-22 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CA2994676A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
ES2773072T3 (es) | 2015-08-28 | 2020-07-09 | Pioneer Hi Bred Int | Transformación de plantas mediada por Ochrobactrum |
WO2017184673A1 (en) | 2016-04-19 | 2017-10-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
EA201892293A1 (ru) | 2016-05-04 | 2019-04-30 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
CN109312359A (zh) | 2016-06-16 | 2019-02-05 | 先锋国际良种公司 | 用以防治昆虫有害生物的组合物和方法 |
MX2018015900A (es) | 2016-06-24 | 2019-04-04 | Pioneer Hi Bred Int | Elementos reguladores de planta y metodos para usarlos. |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN110267527A (zh) | 2016-10-21 | 2019-09-20 | 韦斯塔隆公司 | 可切割肽及包含可切割肽的杀昆虫和杀线虫蛋白 |
CN109863167B (zh) | 2016-11-01 | 2022-12-23 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
US11174295B2 (en) | 2016-12-14 | 2021-11-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US11213028B2 (en) | 2016-12-22 | 2022-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
MX2019009371A (es) | 2017-02-08 | 2019-09-23 | Pionner Hi Bred Int Inc | Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso. |
BR112019019892A2 (pt) | 2017-04-03 | 2020-04-22 | Monsanto Technology Llc | proteínas inibidoras de inseto". |
RU2019140646A (ru) | 2017-05-11 | 2021-06-11 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
WO2019074598A1 (en) | 2017-10-13 | 2019-04-18 | Pioneer Hi-Bred International, Inc. | VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE |
WO2019169150A1 (en) | 2018-03-02 | 2019-09-06 | Pioneer Hi-Bred International, Inc. | Plant health assay |
WO2019178042A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
CA3092075A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US11702668B2 (en) | 2018-05-22 | 2023-07-18 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
CN112566925A (zh) | 2018-08-13 | 2021-03-26 | 先锋国际良种公司 | 新颖的杀昆虫毒素受体和使用方法 |
MX2021002290A (es) | 2018-08-29 | 2021-04-28 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
US20230235352A1 (en) | 2020-07-14 | 2023-07-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
BR112023002603A2 (pt) | 2020-08-10 | 2023-04-04 | Pioneer Hi Bred Int | Elementos reguladores de plantas e métodos de uso dos mesmos |
AR126765A1 (es) * | 2021-08-12 | 2023-11-08 | Syngenta Crop Protection Ag | Composiciones y métodos para controlar insectos coleópteros |
TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
Family Cites Families (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
US5753492A (en) * | 1987-08-12 | 1998-05-19 | Mycogen Corporation | Genes encoding nematode-active toxins from Bacillus thuringiensis strains |
US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
US5990387A (en) | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
US5879918A (en) | 1989-05-12 | 1999-03-09 | Pioneer Hi-Bred International, Inc. | Pretreatment of microprojectiles prior to using in a particle gun |
ES2187497T3 (es) | 1990-04-12 | 2003-06-16 | Syngenta Participations Ag | Promotores preferentemente en tejidos. |
US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
US5932782A (en) | 1990-11-14 | 1999-08-03 | Pioneer Hi-Bred International, Inc. | Plant transformation method using agrobacterium species adhered to microprojectiles |
US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
DE69230290T2 (de) | 1991-08-27 | 2000-07-20 | Novartis Ag, Basel | Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz |
AU670316B2 (en) | 1992-07-27 | 1996-07-11 | Pioneer Hi-Bred International, Inc. | An improved method of (agrobacterium)-mediated transformation of cultured soybean cells |
IL108241A (en) | 1992-12-30 | 2000-08-13 | Biosource Genetics Corp | Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus |
ATE196311T1 (de) | 1993-12-09 | 2000-09-15 | Univ Jefferson | Verbindungen und verfahren zur ortsspezifischen mutation in eukaryotischen zellen |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5545818A (en) * | 1994-03-11 | 1996-08-13 | Calgene Inc. | Expression of Bacillus thuringiensis cry proteins in plant plastids |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
FI104465B (fi) | 1995-06-14 | 2000-02-15 | Valio Oy | Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
DE69933713T2 (de) | 1998-02-26 | 2007-08-23 | Pioneer Hi-Bred International, Inc. | Mais alpha-tubulin 3-18 Promoter |
US6500617B1 (en) * | 1998-05-01 | 2002-12-31 | Maxygen, Inc. | Optimization of pest resistance genes using DNA shuffling |
US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
WO2000028058A2 (en) | 1998-11-09 | 2000-05-18 | Pioneer Hi-Bred International, Inc. | Transcriptional activator lec1 nucleic acids, polypeptides and their uses |
JP4221100B2 (ja) | 1999-01-13 | 2009-02-12 | エルピーダメモリ株式会社 | 半導体装置 |
AU2415200A (en) | 1999-01-18 | 2000-08-01 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
IL138002A0 (en) | 1999-01-19 | 2001-10-31 | Maxygen Inc | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US20020038005A1 (en) * | 2000-01-07 | 2002-03-28 | Wojciechowska Jana Alexandrovna | Novel delta-endotoxins and nucleic acid sequences coding therefor |
AU4964101A (en) | 2000-03-30 | 2001-10-15 | Maxygen Inc | In silico cross-over site selection |
AU2002252974B2 (en) * | 2001-01-09 | 2006-12-14 | BASF Agricultural Solutions Seed US LLC | Bacillus thuringiensis insecticidal proteins |
PT1988099E (pt) * | 2001-01-09 | 2013-01-31 | Bayer Cropscience Nv | Proteínas inseticidas de bacillus thuringiensis |
US20020093147A1 (en) | 2001-01-16 | 2002-07-18 | Berna Michael J. | Well plate seal |
NL1033850C2 (nl) | 2007-05-15 | 2008-11-18 | 3Force B V | Brandersysteem met voorgemengde branders en vlam-overdrachtsmiddelen. |
-
2005
- 2005-02-25 CA CA2556736A patent/CA2556736C/en active Active
- 2005-02-25 CN CN2009101635114A patent/CN101671680B/zh active Active
- 2005-02-25 CN CNA2005800117014A patent/CN1942582A/zh active Pending
- 2005-02-25 AR ARP050100712A patent/AR048744A1/es not_active Application Discontinuation
- 2005-02-25 JP JP2007501041A patent/JP4818252B2/ja not_active Expired - Fee Related
- 2005-02-25 WO PCT/US2005/006238 patent/WO2005082077A2/en active Application Filing
- 2005-02-25 EP EP10000808.5A patent/EP2184360B1/en active Active
- 2005-02-25 EP EP05723907.1A patent/EP1718751B1/en active Active
- 2005-02-25 KR KR1020127006750A patent/KR20120035233A/ko not_active Application Discontinuation
- 2005-02-25 KR KR1020067019428A patent/KR101225918B1/ko not_active IP Right Cessation
- 2005-02-25 US US11/067,557 patent/US7208474B2/en active Active
- 2005-02-25 ES ES10000808.5T patent/ES2552053T3/es active Active
- 2005-02-25 PT PT100008085T patent/PT2184360E/pt unknown
- 2005-02-25 CA CA2799245A patent/CA2799245C/en active Active
- 2005-02-25 BR BRPI0508068-1A patent/BRPI0508068B1/pt active IP Right Grant
- 2005-02-25 HU HUE10000808A patent/HUE028143T2/en unknown
- 2005-02-25 CN CN2009101673582A patent/CN101671681B/zh active Active
- 2005-02-25 AU AU2005216528A patent/AU2005216528B2/en active Active
- 2005-02-25 MX MXPA06009694A patent/MXPA06009694A/es active IP Right Grant
-
2006
- 2006-08-31 ZA ZA200607222A patent/ZA200607222B/en unknown
-
2007
- 2007-02-16 US US11/675,737 patent/US7737331B2/en active Active
- 2007-02-16 US US11/675,729 patent/US7737117B2/en active Active
-
2009
- 2009-05-11 CL CL2009001136A patent/CL2009001136A1/es unknown
- 2009-06-25 AU AU2009202561A patent/AU2009202561B2/en active Active
-
2010
- 2010-04-27 US US12/768,418 patent/US7943734B2/en active Active
- 2010-04-27 US US12/768,464 patent/US7943735B2/en active Active
- 2010-04-28 US US12/768,814 patent/US7943736B2/en active Active
- 2010-04-28 US US12/768,858 patent/US7943737B2/en active Active
-
2011
- 2011-03-18 JP JP2011061374A patent/JP2011182793A/ja not_active Withdrawn
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101671681B (zh) | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 | |
CN102977196B (zh) | 新苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 | |
CA2763879C (en) | Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant |