CN101622938B - Method for cultivating lucid ganoderma - Google Patents
Method for cultivating lucid ganoderma Download PDFInfo
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- CN101622938B CN101622938B CN2009100417076A CN200910041707A CN101622938B CN 101622938 B CN101622938 B CN 101622938B CN 2009100417076 A CN2009100417076 A CN 2009100417076A CN 200910041707 A CN200910041707 A CN 200910041707A CN 101622938 B CN101622938 B CN 101622938B
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Abstract
The invention provides a method for cultivating lucid ganoderma, relating to a method for cultivating edible fungi, in particle to a method for cultivating the lucid ganoderma with new cultivating material. The cultivating material in the method contains eucalyptus, wherein the cultivating material is preferably contains eucalyptus bark or eucalyptus branch or mixture of the eucalyptus bark and the eucalyptus branch. The method selects the eucalyptus as the cultivating material, wherein the eucalyptus is not usually regarded as to be suitable for cultivating the lucid ganoderma, to obtain lucid ganoderma seed entities with faster growth speed, wherein the seed entities almost have the same effective constituent of the lucid ganoderma. Furthermore, the eucalyptus has fast growth, short circulation period and high yield, therefore, the price is lower than the other broad-leaved trees. Because the other constituents such as cotton seed hulls, bagasse, and the like, are not needed to be added, comparatively speaking, the method has lower price.
Description
[technical field]
The present invention relates to a kind of culturing edible fungi, relate in particular to a kind of ganoderma lucidum cultivation method that has used new cultivation material.
[background technology]
Glossy ganoderma is the well-known medicinal fungi of China, and ancient books Shennong's Herbal, " The Master of Preserving Simplicity " and Compendium of Material Medica all have in various degree description and record to the classification of glossy ganoderma and effect.Modern medicine study proves, the polyose in the glossy ganoderma, triterpenes, sterols isoreactivity composition have antitumor, as to regulate immunity, nervous system regulation, the adjusting recycle system etc. function to human body.Therefore domestic the world of medicine is very interested in the research of glossy ganoderma, and all kinds of healthcare products processed goods that contain effective component of glossy ganoderma are also successfully developed, and the enterprise that is engaged in Garnoderma product also arises at the historic moment, and those ganoderma lucidum prods of succeeding in developing are constantly released market.In the middle of whole ganoderma lucidum prod industry, a most primary ring is the cultivation of glossy ganoderma, and the quality of cultivating ganoderma directly influences the quality of its downstream converted products.Learn that according to State Intellectual Property Office of the People's Republic of China's in July, 2009 retrieval the patent relevant with " glossy ganoderma " has 889.But 30 of wherein relevant patent less thaies with the Ganoderma sporophore cultivation.The tame cultivation material of modern glossy ganoderma is with wood chip, the Duan Muwei master of leaf wood.Report with the napier grass cultivating ganoderma was arranged in 2000: it is suitable that water-soluble polysaccharide in the Ganoderma sporophore that grass carries and total amino acid content and wood are planted glossy ganoderma, and mineral element content, lipid acid kind number and vitamin B group are all planted glossy ganoderma a little more than wood, and visible grass carries glossy ganoderma also certain application prospect.In addition, can be used as the cotton seed hulls that also has of cultivation of glossy ganoderma material, but be that the cultivation material easily causes the too high and carbon under-nutrition of nitrogen element nutrition with the cotton seed hulls.In addition, bagasse, Rhizoma Dioscoreae powder, wheat, vinasse etc. all have record to can be used for cultivating ganoderma in some monograph, but still find no the periodical literature support at home.The main marsupial of cultivation of glossy ganoderma mode plants, bottle is planted and segment wood cultivated etc.
In traditional concept and pertinent literature in the past, because eucalyptus contains fragrant hydrocarbon system material, usually eucalyptus and seeds such as the pine that is not suitable for cultivating ganoderma, China fir, camphor tree are equated, think that it also is unwell to cultivating ganoderma.
Along with the development of making plate, paper-making industry, the timber resources demand increases, and adds that State Council has ratified each provinces, autonomous regions and municipalities year forest harvesting limit the Eleventh Five-Year Plan period in 2005, and traditional broad-leaved timber cost that can cause being used for cultivating ganoderma raises; In addition, the seeds of Song Shan one class, it is wooden to contain the material that perfume oil, resin etc. are unfavorable for the glossy ganoderma growth, causes the wood resources acquisition approach that can be used in cultivating ganoderma to become narrow and small and nervous.And, make eucalyptus become a kind of cheap raw material along with a large amount of plantations of eucalyptus in recent years.
[summary of the invention]
The present invention aims to provide a kind of glossy ganoderma growth cycle method for cultivating lucid ganoderma fast, with low cost.
Method for cultivating lucid ganoderma of the present invention contains eucalyptus in its cultivation material.Wherein, the bark or tree bar or the bark tree bar mixture that more preferably contain eucalyptus.And more preferably the cultivation material is the wheat bran of the eucalyptus wood chip of quality percentage composition 73-83%, quality percentage composition 15-25%, the CaCO3 of quality percentage composition 2%.Most preferred cultivation material is the eucalyptus wood chip of quality percentage composition 73-83%, the wheat bran of quality percentage composition 15-25%, the CaCO of quality percentage composition 2%
3
The preferred method for cultivating lucid ganoderma of the present invention may further comprise the steps:
1) gets the raw materials ready: eucalyptus is air-dry and be ground into wood chip;
2) be equipped with water: the water of preparing 1.3 times of cultivation material quality;
3) batching: earlier with wood chip and CaCO
3Mix, add the water of 3/4 quality in the water total mass that goes up the step preparation then, mix, sneak into wheat bran and remainder water again, promptly get and cultivate material;
4) will cultivate the plastics strain bag of expecting to pack into and make cultivating bag, through the High Temperature High Pressure moist heat sterilization, Ganderma lucidum strain is inserted in the cooling back.
5) cultivation went out sesame after 30 days;
6) went out in 50-60 days after the sesame management sophisticated Ganoderma sporophore.
Wherein, selectivity is preferably:
In the step 1) with eucalyptus air-dry be the 40-50% of eucalyptus dry weight to its water content; Be crushed to the wood chip diameter less than 4mm.
Cultivating bag described in the step 4) is the polypropylene strain bag of 15cm * 30cm or 17cm * 33cm; Every sacked material dry weight is 400g or 500g.
Cultivation described in the step 5) is that the lucifuge blow-by is cultivated under 24-26 ℃ of temperature, 55-65% humidity.
The temperature that goes out the sesame management described in the step 6) is 27-30 ℃, and humidity is 85-90%.
The eucalyptus that the present invention has selected for use those skilled in the art to think in method for cultivating lucid ganoderma to be unsuitable for cultivation of glossy ganoderma has always obtained speed of growth Ganoderma sporophore faster as the cultivation material, and wherein contained effective component of glossy ganoderma content is approaching in the sporophore.In addition, because the eucalyptus growth is fast, felling cycle is short, timber yield is high, so its price is lower than other broadleaf tree, and owing to need not add other compositions such as cotton seed hulls, bagasse, therefore method for cultivating lucid ganoderma cost of the present invention is cheaper comparatively speaking.
Adopt the no earthing bag mode of planting to plant in the preferred embodiment of the invention, therefore can under clean environment, carry out cultivation management, also can save shared soil and space simultaneously.
[embodiment]
Embodiment one uses method for cultivating lucid ganoderma of the present invention to prepare Ganoderma sporophore
1) get the raw materials ready: with eucalyptus bark tree bar mixture air-dry to its water content be the 40-50% of eucalyptus dry weight, be crushed to the wood chip diameter then less than 4mm; And the eucalyptus bark of preparing 73-83% (quality percentage composition) is set bar mixture wood chip, the wheat bran of 15-25% (quality percentage composition), the CaCO of 2% (quality percentage composition)
3
2) be equipped with water: the water of preparing 1.3 times of cultivation material quality;
3) batching: earlier with wood chip and CaCO
3Mix, add the water of 3/4 quality in the water total mass that goes up the step preparation then, mix, sneak into wheat bran and remainder water again, promptly get and cultivate material;
4) will cultivate material and pack in the polypropylene strain bag of 15cm * 30cm or 17cm * 33cm, wherein every sacked material dry weight is 400g or 500g, burrows in Bag Material with small wood then, at the bottom of the hole is deep to bag, put plastic hoop at sack then, cover supporting lid, promptly obtain the cultivating bag that makes.Cultivating bag inserts Ganderma lucidum strain after cooling through 126 ℃, 1.0kPa normal atmosphere, High Temperature High Pressure moist heat sterilization 120 minutes.
5) 24-26 ℃ temperature, under the humidity of 55-65%, the lucifuge blow-by was cultivated 30 days;
6) open the cultivating bag lid then, the cultivating bag vertical setting of types is placed (bag with bag between should leave the space) in 27-30 ℃, the environment of relative humidity 85-90%, illumination every day 10 hours, intensity of illumination 300-500lx, and ventilation every day; When glossy ganoderma grows young sesame, should keep spray water smoke 2 times every day to young sesame; Go out sesame management 50-60 days like this, can cultivate Ganoderma sporophore.This moment, the transverse growth of Ganoderma sporophore cap finished, and cap edge white edge disappears.
Embodiment two comparative experiments examples
Bacterial classification: Han Zhi (edible mushrooms researchdevelopment center, Guangdong Microbes Inst)
Eucalypt species: the blue gum in fast growing forest zone, Guangdong
The cultivation material:
| 1 group | Mallee bark chip 83% (quality percentage composition), wheat bran 15% (quality percentage composition), CaCO 32% (quality percentage composition) |
| 2 groups | Eucalyptus pole stock bits 83% (quality percentage composition), wheat bran 15% (quality percentage composition), CaCO 32% (quality percentage composition) |
| 3 groups | Mallee bark tree bar mixing chip 83% (quality percentage composition), wheat bran 15% (quality percentage composition), CaCO 32% (quality percentage composition) |
| 4 groups | Other hardwood sawdusts 83% (quality percentage composition), wheat bran 15% (quality percentage composition), CaCO 32% (quality percentage composition) |
With 30 bacterium bags of above-mentioned four groups of cultivation material the method according to this invention cultivation, treat out the sesame management after 50 days, relatively Zai Pei result respectively.
| Group number | 1 | 2 | 3 | 4 |
| Sporophore bag counterpoise (g) | 35.38 | 38.44 | 37.92 | 39.57 |
| Sporophore mean diameter (cm) | 10.2 | 11.2 | 11.7 | 8.7 |
| Polysaccharide content (%) | 0.962. | 1.013 | 1.064 | 0.696 |
| Total triterpene contents (%) | 0.088 | 0.120 | 0.152 | 0.171 |
| The sporophore profile | Fig. 1 | Fig. 2 | Fig. 3 | Fig. 4 |
As above shown in the table, utilize the eucalyptus wood chip to come cultivating ganoderma, every bag of average sporophore fresh weight is a little less than utilizing other leaf wood wood chips to cultivate, but gap only is within the 5g, shows that it is feasible utilizing the eucalyptus wood chip to replace other leaf wood wood chips.In addition, use eucalyptus bark chip cultivating ganoderma separately, the average fresh weight of its sporophore to be lower than independent use tree pole stock bits with skin bar blended, and the average fresh weight of sporophore that uses tree pole stock bits and the mixing of skin bar to be cultivated separately is very approaching.
In addition, by Fig. 1-4 as seen, four groups of Ganoderma sporophore difference in size and little.The glossy ganoderma that cultivates at " other hardwood sawdusts " as seen, parton entity cap still has white edge, shows this still prematurity of parton entity, occurs and there is white edge on the Ganoderma sporophore that other three kinds of eucalyptus wood chips are cultivated substantially.This shows identical to go out sesame on the time, the speed of growth of the Ganoderma sporophore of eucalyptus cultivation on sawdust is slightly faster than other leaf wood wood chips.Simultaneously, little with the effective constituent difference of the glossy ganoderma of the glossy ganoderma of eucalyptus bits cultivation and other broad-leaved cultivation on sawdusts, polysaccharide content even be higher than the glossy ganoderma that other broad-leaved wood chips are cultivated wherein.
Claims (8)
1. a method for cultivating lucid ganoderma is characterized in that cultivating material and is the wheat bran of the eucalyptus wood chip of quality percentage composition 73-83%, quality percentage composition 15-25%, the CaCO of quality percentage composition 2%
3Described method may further comprise the steps:
1) gets the raw materials ready: eucalyptus is air-dry and be ground into wood chip;
2) be equipped with water: the water of preparing 1.3 times of cultivation material quality;
3) batching: earlier wood chip is mixed with CaCO3, add then and go up the water that goes on foot 3/4 quality in the water total mass of preparing, mix, sneak into wheat bran and remainder water again, promptly get and cultivate material;
4) will cultivate the plastics strain bag of expecting to pack into and make cultivating bag, through the High Temperature High Pressure moist heat sterilization, Ganderma lucidum strain is inserted in the cooling back;
5) cultivation went out sesame after 30 days;
6) went out in 50-60 days after the sesame management sophisticated Ganoderma sporophore.
2. cultivation as claimed in claim 1 is characterized in that described eucalyptus is bark or tree bar or the bark tree bar mixture of eucalyptus.
3. cultivation as claimed in claim 2 is characterized in that cultivating material and is the wheat bran of the eucalyptus wood chip of quality percentage composition 83%, quality percentage composition 15%, the CaCO of quality percentage composition 2%
3
4. cultivation as claimed in claim 3, it is characterized in that in the step 1) with eucalyptus air-dry be the 40-50% of eucalyptus dry weight to its water content.
5. cultivation as claimed in claim 4 is characterized in that in the step 1) eucalyptus being crushed to the wood chip diameter less than 4mm.
6. cultivation as claimed in claim 4 is characterized in that the cultivating bag described in the step 4) is the polypropylene strain bag of 15cm * 30cm or 17cm * 33cm, and each cultivating bag charging dry weight is 400g or 500g.
7. cultivation as claimed in claim 4 is characterized in that the cultivation described in the step 5) is that the lucifuge blow-by is cultivated under 24-26 ℃ of temperature, 55-65% humidity.
8. cultivation as claimed in claim 4 is characterized in that the temperature that goes out the sesame management described in the step 6) is 27-30 ℃, and humidity is 85-90%.
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Cited By (1)
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| TWI453025B (en) * | 2012-06-20 | 2014-09-21 |
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| CN102648685A (en) * | 2012-04-28 | 2012-08-29 | 华南农业大学 | Chinese mushroom cultivation method and preparation method for special culture medium |
| CN102765988B (en) * | 2012-07-17 | 2014-05-07 | 黄山旅游集团佳龙绿色食品有限公司 | Lucid Ganoderma cultivation bag material and method for cultivating Lucid Ganoderma by same |
| CN103229664A (en) * | 2013-04-18 | 2013-08-07 | 黄永红 | Method for planting sweet lucid ganoderma by usage of uncrushed tea seed shells |
| CN103420731B (en) * | 2013-08-15 | 2015-01-21 | 邬金飞 | Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material |
| CN104322270A (en) * | 2013-11-30 | 2015-02-04 | 钟诚 | Cultivation method of lucid ganoderma |
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| CN104082033B (en) * | 2014-06-10 | 2017-01-18 | 原一（厦门）食用菌科技有限公司 | Ganoderma lucidum planting method |
| CN105272512A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Eucalypt wood chip culture medium suitable for growth of edible fungi |
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| CN115250829A (en) * | 2018-03-05 | 2022-11-01 | 广西壮族自治区农业科学院微生物研究所 | Method for cultivating lucid ganoderma by taking eucalyptus processing residues as main cultivation material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI453025B (en) * | 2012-06-20 | 2014-09-21 |
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Address after: 510663 Shenzhou road 788, Science Development Zone, Guangzhou Development Zone, Guangzhou, Guangdong, Luogang District Patentee after: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee after: Institute of Microbiology, Guangdong Academy of Sciences Address before: 510663 Shenzhou road 788, Science Development Zone, Guangzhou Development Zone, Guangzhou, Guangdong, Luogang District Patentee before: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee before: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Address after: 510663 Shenzhou road 788, Science Development Zone, Guangzhou Development Zone, Guangzhou, Guangdong, Luogang District Patentee after: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee after: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Address before: 510663 Shenzhou road 788, Science Development Zone, Guangzhou Development Zone, Guangzhou, Guangdong, Luogang District Patentee before: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee before: GUANGDONG INSTITUTE OF MICROBIOLOGY |