CN101592606A - Portable surface plasma resonance biochemical analyzer - Google Patents
Portable surface plasma resonance biochemical analyzer Download PDFInfo
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- CN101592606A CN101592606A CN 200810113244 CN200810113244A CN101592606A CN 101592606 A CN101592606 A CN 101592606A CN 200810113244 CN200810113244 CN 200810113244 CN 200810113244 A CN200810113244 A CN 200810113244A CN 101592606 A CN101592606 A CN 101592606A
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Abstract
A kind of portable surface plasma resonance biochemical analyzer of the present invention comprises optical system, signal picker, surface plasma resonance chip and response measurement pond, the auto injection system for the distribution of commodities and operation control and data handling system.Light source, lens combination, prism and line array CCD are fixed on the whole optical table support.On plane on the level of triangle optical prism, surface plasma resonance chip and little measuring cell are arranged, little measuring cell is stacked in resonance chip upper horizontal, and input, efferent duct are arranged in little measuring cell, and the other end of input, efferent duct is connected with automatic sample handling system.Optical system, signal picker, sampling system are electrically connected with embedded microcontroller respectively, control Biochemical Analyzer with single-chip microcomputer, the monitoring light intensity changes, and measure SPR characteristic parameter curve, testing result shows on computing machine or LCDs, can send to command centre by radio sensing network, realize on-the-spot the detection or wireless no people detection.
Description
Technical field
The present invention relates to the detection technique field, is a kind of miniaturization, portable surface plasma resonance (SPR) Biochemical Analyzer.
Background technology
(Surface Plasmon Resonance, SPR) sensing technology is an emerging biological chemistry detection technique to surface plasma resonance.Since Nylander and Liedberg are used for gas detection and biology sensor with surface plasma resonance (SPR) sensing technology first in nineteen eighty-two, over 20 years, the SPR sensing technology has all obtained development at full speed in implementation, instrument development and application expansion.
Surface plasma resonance (SPR) Biochemical Analyzer is bulky usually both at home and abroad at present, and needs computing machine to carry out signal and data processing, generally is applicable to lab analysis research, can not satisfy the on-the-spot needs that detect.In order to adapt to the middle at the scene needs that detect in multiple field, miniaturization, portable surface plasmon resonance biosensor begin to occur abroad, but need mostly and the computing machine coupling, can't use separately.
Miniaturization, portable surface plasma resonance biochemical analyzer not only can be so that price reduce greatly, and easy to use, and range of application is greatly expanded.In addition, the SPR Biochemical Analyzer after the microminiaturization also can improve a lot on stability and reliability.
Summary of the invention
The objective of the invention is to propose a kind of portable surface plasma resonance (SPR) Biochemical Analyzer, adopt fixed support light path system, surface plasma resonance (SPR) chip, high-resolution linear array CCD detection, automatic sample handling system and system operation control and data handling system, realize on-the-spot or wireless, unmanned surface plasma resonance (SPR) quantitative test, external, domestic not being reported of the surface plasma resonance of this novel miniaturization (SPR) biochemical analyzer.
For achieving the above object, technical solution of the present invention is:
A kind of portable surface plasma resonance biochemical analyzer comprises optical system, signal acquiring system, surface plasma resonance chip and little measuring cell, the auto injection system for the distribution of commodities and operation control and data handling system; Its optical system comprises laser or led light source, lens combination, prism, signal receiver; The integral support lower end is fixed in the Biochemical Analyzer pedestal, and prism is fixed on plane on the integral support through optical table, on one side plane parallel on plane that prism constitutes and the integral support; Light source is fixed on the fixed arm on plane on the integral support, and lens combination is fixed on the LASER Light Source ejecting end, and signal receiver is fixed on another fixed arm on plane on the integral support; LASER Light Source is through the outgoing beam of the lens combination plane of incidence to prism, select optimal incident angle in fact each other after, signal receiver is located on the reflected light path of prism, forms between the three and stablizes firm positional structure;
On plane on the level of prism, be provided with surface plasma resonance chip and little measuring cell, little measuring cell is stacked in resonance chip upper horizontal, is connected with input pipe and efferent duct on little measuring cell, and the other end of input pipe and efferent duct is connected with automatic sample handling system;
Optical system, signal acquiring system, automatic sample handling system are electrically connected with embedded microcontroller respectively.
Described surface plasma resonance biochemical analyzer, its described operation control and data handling system, for embedding the microcontroller processing mode, preloaded system operating control procedure and data acquisition process program in microcontroller, the control Biochemical Analyzer, monitor signal receptacle light intensity changes, and measures the SPR characteristic curve; Deal with data, and testing result shown on LCDs, or send the testing result signal to command centre by the node of radio sensing network, realize on-the-spot the detection or wireless no people detection;
Its circuit is:
The signal receiver output terminal is electrically connected amplifier module, AD chip, storer in proper order, and AD chip, storer are electrically connected with CPLD respectively; The signal receiver input end is electrically connected with CPLD; CPLD is electrically connected with microcontroller; Microcontroller has LCDs and network node.
Described surface plasma resonance biochemical analyzer, its described operation control and data handling system, be computer processing mode, by the RS232 port embedded microcontroller is linked to each other with computing machine, computing machine is equipped with system operation Control Software and data acquisition process software in advance, control Biochemical Analyzer, monitor signal receptacle light intensity changes, and measures the SPR characteristic curve; Deal with data, and testing result shown on computers, realize on-the-spot the detection;
Its circuit is:
The signal receiver output terminal is electrically connected amplifier module, AD chip, storer in proper order, and AD chip, storer are electrically connected with CPLD respectively; The signal receiver input end is electrically connected with CPLD; CPLD is electrically connected with microcontroller; Microcontroller is electrically connected with computing machine through the RS232 port.
Described surface plasma resonance biochemical analyzer, its described optical system, its light source forms the wedge shape light beam through lens combination, injects prism with fixed angle, penetrates with fixed angle behind prism again, impinges upon on the signal receiver.
Described surface plasma resonance biochemical analyzer, its described signal receiver is line array CCD.
Described surface plasma resonance biochemical analyzer, its described little measuring cell is the little measuring cell of making of PDMS silicon rubber or other encapsulants of sealing, the measuring cell raceway groove is positioned at the center and becomes in-line.
Described surface plasma resonance biochemical analyzer, its described automatic sample handling system, the system for micropump, pipeline, the sample storage container of wriggling Micropump or prepare based on the MEMS technology are connected drives sample introduction with Micropump.
Described surface plasma resonance biochemical analyzer, its described microcontroller is single-chip microcomputer.
Apparatus of the present invention not only have the distinct advantages of surface plasma resonance (SPR) biochemical analysis technology: sample need not mark and purifying, can detect muddy or opaque fluid sample, can Real-time and Dynamic Detection etc., and this portable SPR Biochemical Analyzer volume reduces greatly, be easy to carry, cost reduces greatly, can realize on-the-spot the detection, for general surface plasma resonance (SPR) Biochemical Analyzer does not possess.SPR Biochemical Analyzer particularly of the present invention also can adopt the Single-chip Controlling Biochemical Analyzer and gather and processing signals, can use separately by divorced from computer, also can be used for the unattended use of radio sensing network, being outstanding innovation of the present invention, is the significant development to present surface plasma resonance (SPR) Biochemical Analyzer function; System has disposed automatic sample handling system again, has realized the robotization detection, has more advanced measuring technology and application fields more.
Description of drawings
Fig. 1. be portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer light path system and system for the distribution of commodities structural representation, this structure is to integrate to form fixedly one-piece construction;
Fig. 2. be that the present invention adopts the single-chip microcomputer mode to operate the SPR-2006 type portable surface plasma resonance biochemical analyzer electrical block diagram of control and data processing;
Fig. 3. be that the present invention adopts computer mode to operate the SPR-2005 type portable surface plasma resonance biochemical analyzer electrical block diagram of control and data processing;
Fig. 4. be a typical SPR peak curve of portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer;
Fig. 5, be the sensitivity test experimental result picture of portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer;
Fig. 6. be portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer test human IgG experimental result picture.
Embodiment
As shown in Figure 1, be system light path of the present invention and system for the distribution of commodities structural representation.On the horizontal plane of prism 3, be provided with surface plasma resonance (SPR) sensor chip 5 and little measuring cell 4, little measuring cell 4 is stacked in sensor chip 5 upper horizontal, be connected with input pipe and efferent duct on little measuring cell 4, the other end of input pipe and efferent duct is connected with automatic sample handling system (with Micropump or pony pump), so that testing sample is pumped into little measuring cell 4.Prism 3 is fixed on the integral support, and laser or led light source 1 are fixed on the fixed arm of integral support, and lens combination 2 is fixed on laser or the led light source 1, and signal receiver line array CCD 6 is fixed on another fixed arm of integral support.Laser or led light source 1 are through the outgoing beam of lens combination 2 plane of incidence to prism 3, after selecting optimal incident angle in fact each other, signal receiver line array CCD 6 is located on the reflected light path of prism 3, make the three form stable strong construction, can realize on-the-spot surface plasma resonance (SPR) quantitative test.
This design, the resonant angle of having fixed surface plasma resonance (SPR) Biochemical Analyzer makes the maximum miniaturization of surface plasma resonance (SPR) Biochemical Analyzer, and sound construction is to obtain portable surface plasma resonance biochemical analyzer.
As shown in Figure 2, be that the present invention adopts the single-chip microcomputer mode to operate the SPR-2006 type portable surface plasma resonance biochemical analyzer electrical block diagram of control and data processing.The light intensity of signal receiver line array CCD 6 is simulating signals, it also is the SPR characteristic signal of measuring, this simulating signal is through 14 AD chips of input after the amplification of high-operational amplifier, utilize CPLD control AD conversion, when scanning, CPLD provides address ram, and the digital signal that AD changes is out sent in the RAM at twice by CPLD control.CPLD also provides CCD6 required control signal in addition.Write-in program in microcontroller (being single-chip microcomputer), control SPR Biochemical Analyzer, deal with data, and result transmission gone up to LCDs (figure do not illustrate) show or consequential signal is sent it back radio sensing network command centre (figure does not illustrate) by network node.
As shown in Figure 3, be the SPR-2005 type portable surface plasma resonance biochemical analyzer electrical block diagram that adopts computer mode to operate control and data processing of the present invention.Be not both with Fig. 2 and be, adopt the R232 port that microcontroller (being single-chip microcomputer) and computing machine are connected, the effect of single-chip microcomputer is just accepted acquisition instructions and is sent the data among the RAM to COMPUTER CALCULATION from computing machine.The effect of computing machine is the control Biochemical Analyzer, deal with data, and test result shown in computing machine.
Figure 4 shows that a typical SPR peak curve of portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer.
Figure 5 shows that the sensitivity test experimental result picture of portable surface plasma resonance of the present invention (SPR) Biochemical Analyzer, join glycerine 0～No. 10, the percent concentration interval is a water 2%, No. 0, and No. 1 is 2% glycerine, and No. 2 is 4% glycerine, by that analogy.The detection sensitivity of the portable SPR Biochemical Analyzer of the present invention reaches 5.4 * 10 approximately
-5RIU (refractive index unit).
Figure 6 shows that the test data curve that adopts the present invention's " SPR-2005 type " portable surface plasma resonance (SPR) Biochemical Analyzer test human IgG.The experiment of test human IgG is antibody-antigen immune reaction experiment.Under certain condition the goat anti-human igg can with the human IgG generation immune response that is fixed on the chip on, the reaction before and after pixel value (refractive index) change, can onlinely detect this variations in refractive index by the present invention, thereby obtain immunoreactive result.Each stage implication is as follows in the test curve: (A) feed the HBS buffer solution for cleaning.(B) activation of carboxyl: use contain 0.2mol/LEDC (N-ethyl-N '-(3-dimethylaminopropyl) carbodiimidehydrochloride, C
8H
17N
3HCl, 191.70), 0.05mol/L NHS (N-hydroxysuccinimide, C
4H
5NO
3, 115.09) aqueous solution carboxylic group activation is the N-hydroxy-succinamide ester, feed the albumen (pH is lower than its isoelectric point, so that its positively charged, the damping fluid of low ionic strength is cooked solvent) that will fix then.(C) feed human IgG (acetate buffer solution).(D) ethanolamine hydrochloric salt solution (regulating its pH to the 8.5) deactivation of feeding 1mol/L with NaOH.(E) feed the goat anti-human igg and react a period of time.(F) clean the back and (A) the stage curve compared significant change, illustrate that immune response has taken place for goat anti-human igg and human IgG.Therefore, instrument of the present invention can detect the 1mg goat anti-human igg.
Claims (8)
1, a kind of portable surface plasma resonance biochemical analyzer comprises optical system, signal acquiring system, surface plasma resonance chip and little measuring cell, the auto injection system for the distribution of commodities and operation control and data handling system; It is characterized in that optical system comprises laser or led light source, lens combination, prism, signal receiver; The integral support lower end is fixed in the Biochemical Analyzer pedestal, and prism is fixed on plane on the integral support through optical table, on one side plane parallel on plane that prism constitutes and the integral support; Light source is fixed on the fixed arm on plane on the integral support, and lens combination is fixed on the light source ejecting end, and signal receiver is fixed on another fixed arm on plane on the integral support; Light source is through the outgoing beam of the lens combination plane of incidence to prism, select optimal incident angle in fact each other after, signal receiver is located on the reflected light path of prism, forms between the three and stablizes firm positional structure;
On plane on the level of prism, be provided with surface plasma resonance chip and little measuring cell, little measuring cell is stacked in resonance chip upper horizontal, is connected with input pipe and efferent duct on little measuring cell, and the input pipe other end is connected with automatic sample handling system;
Optical system, signal acquiring system, automatic sample handling system are electrically connected with embedded microcontroller respectively.
2, surface plasma resonance biochemical analyzer as claimed in claim 1, it is characterized in that, described operation control and data handling system, for embedding the microcontroller processing mode, preloaded system operating control procedure and data acquisition process program in microcontroller, the control Biochemical Analyzer, monitor signal receptacle light intensity changes, and measures the SPR characteristic curve; Deal with data, and testing result shown on LCDs, or send the testing result signal to command centre by the node of radio sensing network, realize on-the-spot the detection or wireless no people detection;
Its circuit is:
The signal receiver output terminal is electrically connected amplifier module, AD chip, storer, and AD chip, storer are electrically connected with CPLD respectively; The signal receiver input end is electrically connected with CPLD; CPLD is electrically connected with microcontroller; Microcontroller has LCDs and network node.
3, surface plasma resonance biochemical analyzer as claimed in claim 1, it is characterized in that, described operation control and data handling system, be computer processing mode, by the RS232 port embedded microcontroller is linked to each other with computing machine, computing machine is equipped with system operation Control Software and data acquisition process software in advance, controls Biochemical Analyzer, monitor signal receptacle light intensity changes, and measures the SPR characteristic curve; Deal with data, and testing result shown on computers, realize on-the-spot the detection;
Its circuit is:
The signal receiver output terminal is electrically connected amplifier module, AD chip, storer, and AD chip, storer are electrically connected with CPLD respectively; The signal receiver input end is electrically connected with CPLD; CPLD is electrically connected with microcontroller; Microcontroller is electrically connected with computing machine through the RS232 port.
4, surface plasma resonance biochemical analyzer as claimed in claim 1 is characterized in that, described optical system, its light source forms the wedge shape light beam through lens combination, inject prism with fixed angle, behind prism, penetrate with fixed angle again, impinge upon on the signal receiver.
5,, it is characterized in that described signal receiver is line array CCD as claim 1,2,3 or 4 described surface plasma resonance biochemical analyzers.
6, surface plasma resonance biochemical analyzer as claimed in claim 1 is characterized in that, described little measuring cell is the little measuring cell of making of PDMS silicon rubber or other encapsulants of sealing, and the measuring cell raceway groove is positioned at the center and becomes in-line.
7, surface plasma resonance biochemical analyzer as claimed in claim 1 is characterized in that, described automatic sample handling system, and the system for micropump, pipeline, the sample storage container of wriggling Micropump or prepare based on the MEMS technology are connected drives sample introduction with Micropump.
8,, it is characterized in that described microcontroller is single-chip microcomputer as claim 1,2 or 3 described surface plasma resonance biochemical analyzers.
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| CN 200810113244 CN101592606B (en) | 2008-05-28 | 2008-05-28 | Portable surface plasma resonance biochemical analyzer |
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| CN 200810113244 CN101592606B (en) | 2008-05-28 | 2008-05-28 | Portable surface plasma resonance biochemical analyzer |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103454254A (en) * | 2013-09-09 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Detection unit of surface plasmon resonance (SPR) instrument |
| CN105300931A (en) * | 2015-10-14 | 2016-02-03 | 温州生物材料与工程研究所 | Portable surface plasma resonance biochemical detection device based on intelligent mobile phone as well as portable mobile phone accessories and application thereof |
| CN105717071A (en) * | 2016-02-19 | 2016-06-29 | 清华大学 | Surface plasma resonance sensing chip and cell response detection system and method |
| CN106501219A (en) * | 2016-10-19 | 2017-03-15 | 中国科学院电子学研究所 | A kind of Portable, multiple parameter surface plasma resonance biochemical analyzer |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3399836B2 (en) * | 1998-05-21 | 2003-04-21 | 富士写真フイルム株式会社 | Surface plasmon sensor |
| US6326612B1 (en) * | 1998-10-13 | 2001-12-04 | Texas Instruments Incorporated | System and method for optical sensing utilizing a portable, detachable sensor cartridge |
| CN1715878A (en) * | 2005-07-07 | 2006-01-04 | 浙江大学 | Double light beam detection modulation device based on surface plasma wave sensing |
| CN101059436A (en) * | 2007-04-16 | 2007-10-24 | 浙江大学 | Non-scanning type intelligent digitalized integrated SPR detector |
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2008
- 2008-05-28 CN CN 200810113244 patent/CN101592606B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103454254A (en) * | 2013-09-09 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Detection unit of surface plasmon resonance (SPR) instrument |
| CN105300931A (en) * | 2015-10-14 | 2016-02-03 | 温州生物材料与工程研究所 | Portable surface plasma resonance biochemical detection device based on intelligent mobile phone as well as portable mobile phone accessories and application thereof |
| CN105717071A (en) * | 2016-02-19 | 2016-06-29 | 清华大学 | Surface plasma resonance sensing chip and cell response detection system and method |
| CN105717071B (en) * | 2016-02-19 | 2018-08-17 | 清华大学 | Surface plasmon resonance sensing chip and cellular response detecting system and method |
| CN106501219A (en) * | 2016-10-19 | 2017-03-15 | 中国科学院电子学研究所 | A kind of Portable, multiple parameter surface plasma resonance biochemical analyzer |
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Assignee: Beijing Liuzhou Yicheng electromechanical installation Co. Ltd. Assignor: Institute of Electronics, Chinese Academy of Sciences Contract record no.: 2011990001112 Denomination of invention: Portable surface plasma resonance biochemical analyzer Granted publication date: 20110907 License type: Exclusive License Open date: 20091202 Record date: 20111223 |