CN1715878A - Double light beam detection modulation device based on surface plasma wave sensing - Google Patents
Double light beam detection modulation device based on surface plasma wave sensing Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 230000003287 optical effect Effects 0.000 claims abstract description 6
- 230000008020 evaporation Effects 0.000 claims description 4
- 238000001704 evaporation Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000008016 vaporization Effects 0.000 abstract 1
- 238000009834 vaporization Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010304 firing Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 238000001755 magnetron sputter deposition Methods 0.000 description 1
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- 230000009897 systematic effect Effects 0.000 description 1
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Abstract
The double light beam detection and modulation device based on surface plasma wave sensing has laser, optical assembly to split the laser beam into the first polarized light beam and the second polarized light beam, prism with vaporization coated Au film, grown biosensitive film and pond for the measured sample to flow in to total reflect the incident two light beams, and the first PD detector and the second PD detector to receive these two reflected light beams separately. The present invention has the characteristic of surface plasma resonance (SPR) sensing technology maintained, and the double light beam SPR sensors, which need no precise scanning incident angle and thus simplified angle regulating mechanism, less measurement error and increased operation performance. The detection of double light beam light strength signal may be completed with cheap photodiode, and this can simplify the system, lower the manufacture cost and make the instrument portable.
Description
Technical field
The present invention relates to biosensor technique, photoelectric detecting technology, relate in particular to double light beam detection modulation device based on surface plasma wave sensing.
Background technology
The detection modulator approach of general surface plasma resonance (SPR) has following four kinds usually:
1. angular modulation changes the incident angle θ of incident light wave, under a certain tested refractive index, according to equation with the light incident of fixing wavelength X
Always can find an incident angle θ, make it to satisfy resonant condition, produce surface plasma resonance.When the refractive index of sample changes, rescan incident angle, seek the resonance incident angle, set up the relation between resonance incident angle and the sample refractive index.The detection resolution of angular modulation method can reach 1 * 10
-7Deg RIU
-1(RIU is a refractive index unit).
2. wavelength-modulated changes the wavelength X of incident light wave with fixing incident angle θ incident.According to the dispersive model of Drude, for spr sensor based on prism, the metal refractive index ε that different lambda1-wavelengths is corresponding different
mRefractive index ε with prism
PrWhen refractive index one timing of sample, the scanning lambda1-wavelength makes it to satisfy resonant condition, produces surface plasma resonance, sets up the relation between resonance lambda1-wavelength and the sample refractive index.The detection resolution and the angular modulation of wavelength-modulated are basic identical, relatively are suitable for waveguide or optical fiber surface plasmon detection scheme.Usually incident light often adopts white light source, and pick-up unit is with spectrometer or CCD device.
3. intensity modulated is measured the light intensity that produces when variations in refractive index and is changed with fixing incident angle θ, fixing incident wavelength λ incident, sets up the relation between transmission light intensity and the refractive index.The intensity modulated detection method, structure is very simple, and the pick-up unit price is also very cheap, but the poor stability of system, can't distinguish and detect the light intensity signal variation is that error that caused by variations in refractive index or pick-up unit produces, and the anti-interference of detection system is bad and detection sensitivity is low.
4. phase modulation (PM) is scanned into firing angle θ earlier, seek the refractive index one surface plasma resonance incident angle regularly of sample, incident θ when fix resonance this moment, measure the resonance phase place under this refractive index then, when the variations in refractive index of sample, repeat said process, setting up resonates concerns between phase place and the refractive index, just can obtain the refractive index size of sample according to this relation.Phase modulation (PM) is a kind of detection method that has development potentiality, and it has signal to noise ratio (S/N ratio) and higher detection sensitivity preferably, and shortcoming is the pick-up unit complexity of system, is difficult to realize the portability of surface plasma resonance sensor apparatus.
Angular modulation, wavelength-modulated and intensity modulated technology are very ripe, and these technology extensively adopt in surface plasma resonance (SPR) sensing testing instrument and designed monitoring analysis system.Although angle adjusting method accuracy of detection height because surface plasma resonance angular modulation method needs the high-precision angular adjustment apparatus of a cover, is inconvenient to be used for on-the-spot on-line monitoring etc.Patent of the present invention is after the detection technique of surface plasma resonance sensor is carried out systematic analysis and experimental study, and a kind of twin-beam based on the surface plasma wave sensing principle of proposition detects modulation technique.The twin-beam of surface plasma wave sensing device detects modulation principle: adopt two and be fixed into firing angle θ
1And θ
2Incident, the two-beam ripple produces reflection after affacting the interphase of the metal A u film of prism end face and measured matter, detect its reflective light intensity with two photodetectors respectively, set up the relation between two bundle reflective light intensities and the sample refractive index, promptly can obtain the value of the refractive index of sample.Double light beam detection modulation device need not to carry out angle scanning in test, only need adjust the angle of incident light before detection according to calculated value.In the twin-beam detection system, replaced normally used spectrometer or light power meter to make the reflected light signal detector with photodiode, therefore simplify the complicacy of system greatly, reduced system's manufacturing cost, satisfied the high-resolution requirement of detection system.
Summary of the invention
The purpose of this invention is to provide a kind of resolution of sensor and double light beam detection modulation device based on surface plasma wave sensing of measuring accuracy of not reducing.
It has laser instrument, laser instrument is divided into first light beam, second light beam, two bundle polarized lights by optical module, incide prism, evaporation has the Au film on the prism end face, growth has bio-sensitive film on the Au film, bio-sensitive film is provided with the sample flow cell, produces total reflection at the prism end face, and two bundle reflected light enter into a PD detector, the 2nd PD detector respectively.
The thickness of Au film is 40～60nm.Prism is a K9 glass isoceles triangle prism.
The present invention had both kept the characteristics of surface plasma resonance (SPR) sensing technology, simultaneously again because the twin-beam spr sensor does not need accurate scanning incident angle, simplified angle-adjusting mechanism, the test error of having avoided movable drive disk assembly to bring has strengthened operability.Because what detect is the light intensity signal of two light beams, can replace spectrometer or expensive ccd array with photodiode, thereby can reduce the complicacy and the manufacturing cost of system greatly, be easy to be designed to portable instrument.
Description of drawings
Accompanying drawing is based on the double light beam detection modulation device structural representation of surface plasma wave sensing, among the figure: laser instrument 1, prism 2, optical module 3, first light beam 4, second light beam 5, a PD photo-detector 6, the 2nd PD photo-detector 7, golden film 8, living beings sensitive membrane 9, sample flow cell 10.
Embodiment
As shown in drawings, double light beam detection modulation device based on surface plasma wave sensing has laser instrument (laser diode) 1, laser instrument is divided into first light beam 4,5 liang of bundles of second light beam polarized light by optical module (comprising beam collimation device, polarizer and beam splitter) 3, incide prism 2, evaporation has Au film 8 on prism 2 end faces, growth has bio-sensitive film 9 on the Au film, bio-sensitive film 9 is provided with sample flow cell 10, produce total reflection at prism 2 end faces, two bundle reflected light enter into a PD detector 6, the 2nd PD detector 7 respectively.The thickness of Au film 8 is 40～60nm.Prism 2 is a K9 glass isoceles triangle prism.
Adopt the Au film 8 of magnetron sputtering evaporation 40～60nm at the prism end face, growth one deck living beings sensitive membrane 9 on the Au film, as the antibody in the immune detection etc., the flow cell 10 that fills sample places the upper surface of Au film 8.The optical module 3 of laser instrument 1 by comprising collimating apparatus, polarizer and beam splitter, produce the two-beam ripple, first light beam 4 and second light beam 5, two light beams incide the end face of prism---the interface of Au film and sample respectively with near the angle the resonance angle, a PD photodetector 6 and the 2nd PD photodetector 7 obtain the catoptrical performance number p (θ of two bundles
1) and p (θ
2).When material analyzed in the sample flow pond 10 combines with bio-sensitive film 9, produce variations in refractive index, calculate the catoptrical power difference DELTA p=|p (θ of two bundles this moment
1)-p (θ
2) |, sample concentration changes the corresponding different respectively Δ p value of variations in refractive index cause, sets up the relation between the refractive index value of Δ p and sample, concerns the concentration value that then can obtain sample by demarcation thus.
Claims (3)
1. double light beam detection modulation device based on surface plasma wave sensing, it is characterized in that, it has laser instrument (1), laser instrument is divided into first light beam (4), second light beam (5) two bundle polarized lights by optical module (3), incide prism (2), evaporation has Au film (8) on prism (2) end face, growth has bio-sensitive film (9) on the Au film, bio-sensitive film (9) is provided with sample flow cell (10), produce total reflection at prism (2) end face, two bundle reflected light enter into a PD detector (6), the 2nd PD detector (7) respectively.
2. a kind of double light beam detection modulation device based on surface plasma wave sensing according to claim 1 is characterized in that the thickness of described Au film (8) is 40～60nm.
3. a kind of double light beam detection modulation device based on surface plasma wave sensing according to claim 1 is characterized in that, described prism (2) is a K9 glass isoceles triangle prism.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510050606 CN1715878A (en) | 2005-07-07 | 2005-07-07 | Double light beam detection modulation device based on surface plasma wave sensing |
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| CN 200510050606 CN1715878A (en) | 2005-07-07 | 2005-07-07 | Double light beam detection modulation device based on surface plasma wave sensing |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779117A (en) * | 2007-07-31 | 2010-07-14 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor device with a light source |
| CN101825568A (en) * | 2010-03-31 | 2010-09-08 | 中国科学院半导体研究所 | Device for detecting medium refraction index change by utilizing spectrum intensity change |
| CN101451952B (en) * | 2008-12-01 | 2011-01-12 | 华东师范大学 | Method implementing wideband optical spectrum surface plasma resonance |
| CN101592606B (en) * | 2008-05-28 | 2011-09-07 | 中国科学院电子学研究所 | Portable surface plasma resonance biochemical analyzer |
| CN101802592B (en) * | 2007-08-20 | 2013-01-09 | 韩国生命工学研究院 | Surface plasmon resonance sensor using rotating mirror |
| CN109916860A (en) * | 2019-04-19 | 2019-06-21 | 广东海洋大学 | A kind of two-beam p-polarization glass prism SPR heavy metal ion sensor and preparation method thereof |
-
2005
- 2005-07-07 CN CN 200510050606 patent/CN1715878A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779117A (en) * | 2007-07-31 | 2010-07-14 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor device with a light source |
| CN101779117B (en) * | 2007-07-31 | 2013-12-04 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor device with a light source |
| CN101802592B (en) * | 2007-08-20 | 2013-01-09 | 韩国生命工学研究院 | Surface plasmon resonance sensor using rotating mirror |
| CN101592606B (en) * | 2008-05-28 | 2011-09-07 | 中国科学院电子学研究所 | Portable surface plasma resonance biochemical analyzer |
| CN101451952B (en) * | 2008-12-01 | 2011-01-12 | 华东师范大学 | Method implementing wideband optical spectrum surface plasma resonance |
| CN101825568A (en) * | 2010-03-31 | 2010-09-08 | 中国科学院半导体研究所 | Device for detecting medium refraction index change by utilizing spectrum intensity change |
| CN109916860A (en) * | 2019-04-19 | 2019-06-21 | 广东海洋大学 | A kind of two-beam p-polarization glass prism SPR heavy metal ion sensor and preparation method thereof |
| CN109916860B (en) * | 2019-04-19 | 2024-02-13 | 广东海洋大学 | Double-beam p-polarization prism SPR heavy metal ion sensor and preparation method thereof |
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