CN101568647B - 无需信号肽从细菌分泌抗体 - Google Patents

无需信号肽从细菌分泌抗体 Download PDF

Info

Publication number
CN101568647B
CN101568647B CN200680034693XA CN200680034693A CN101568647B CN 101568647 B CN101568647 B CN 101568647B CN 200680034693X A CN200680034693X A CN 200680034693XA CN 200680034693 A CN200680034693 A CN 200680034693A CN 101568647 B CN101568647 B CN 101568647B
Authority
CN
China
Prior art keywords
antibody
gene
polypeptide
sequence
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200680034693XA
Other languages
English (en)
Chinese (zh)
Other versions
CN101568647A (zh
Inventor
G·亚兰顿
C·R·贝宾顿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Humanigen Inc
Original Assignee
Kalobios Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kalobios Pharmaceuticals Inc filed Critical Kalobios Pharmaceuticals Inc
Publication of CN101568647A publication Critical patent/CN101568647A/zh
Application granted granted Critical
Publication of CN101568647B publication Critical patent/CN101568647B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Gram-negative bacteria
    • C07K16/1214—Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN200680034693XA 2005-07-22 2006-07-07 无需信号肽从细菌分泌抗体 Expired - Fee Related CN101568647B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US70190205P 2005-07-22 2005-07-22
US60/701,902 2005-07-22
US11/273,906 2005-11-14
US11/273,906 US8211648B2 (en) 2005-07-22 2005-11-14 Secretion of antibodies without signal peptides from bacteria
PCT/US2006/026334 WO2007018853A2 (en) 2005-07-22 2006-07-07 Secretion of antibodies without signal peptides from bacteria

Publications (2)

Publication Number Publication Date
CN101568647A CN101568647A (zh) 2009-10-28
CN101568647B true CN101568647B (zh) 2012-10-03

Family

ID=37679517

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200680034693XA Expired - Fee Related CN101568647B (zh) 2005-07-22 2006-07-07 无需信号肽从细菌分泌抗体

Country Status (11)

Country Link
US (2) US8211648B2 (enExample)
EP (1) EP1907610B1 (enExample)
JP (1) JP5087541B2 (enExample)
KR (1) KR101014891B1 (enExample)
CN (1) CN101568647B (enExample)
AU (1) AU2006276823B2 (enExample)
CA (1) CA2615776C (enExample)
IL (1) IL188889A (enExample)
NZ (1) NZ565126A (enExample)
SG (1) SG177895A1 (enExample)
WO (1) WO2007018853A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8211648B2 (en) * 2005-07-22 2012-07-03 Kalobios Pharmaceuticals, Inc. Secretion of antibodies without signal peptides from bacteria
JO3076B1 (ar) 2007-10-17 2017-03-15 Janssen Alzheimer Immunotherap نظم العلاج المناعي المعتمد على حالة apoe
CN102037016B (zh) 2008-04-28 2015-06-03 卡罗拜奥斯制药公司 针对粒细胞巨噬细胞集落刺激因子的抗体
WO2010091189A1 (en) 2009-02-04 2010-08-12 Kalobios Pharmaceuticals, Inc. Combination antibiotic and antibody therapy for the treatment of pseudomonas aeruginosa infection
WO2010093814A1 (en) 2009-02-11 2010-08-19 Kalobios Pharmaceuticals, Inc. Methods of treating dementia using a gm-csf antagonist
PT2488554T (pt) 2009-10-14 2019-09-13 Humanigen Inc Anticorpos para epha3
AR079336A1 (es) 2009-12-11 2012-01-18 Irm Llc Antagonistas de la pro-proteina convertasa-subtilisina/quexina tipo 9 (pcsk9)
KR20140006022A (ko) 2011-02-11 2014-01-15 아이알엠 엘엘씨 Pcsk9 길항제
WO2012133119A1 (ja) * 2011-03-28 2012-10-04 長瀬産業株式会社 フェリチンの製造方法
WO2012170607A2 (en) 2011-06-10 2012-12-13 Novartis Ag Use of pcsk9 antagonists
WO2012168491A1 (en) 2011-06-10 2012-12-13 Novartis Ag Pharmaceutical formulations of pcsk9 antagonists
MX2014010495A (es) * 2012-03-02 2014-11-14 Ablynx Nv Polipeptidos que enlazan a pcrv.
WO2014084859A1 (en) 2012-11-30 2014-06-05 Novartis Ag Molecules and methods for modulating tmem16a activities
WO2014116051A1 (ko) * 2013-01-24 2014-07-31 스크립스코리아 항체연구원 단백질 조합 기반의 Fv 라이브러리 및 이의 제조 방법
US10328149B2 (en) * 2014-06-13 2019-06-25 Tenboron Oy Conjugates comprising an anti-EGFR1 antibody
RU2609627C2 (ru) * 2014-09-26 2017-02-02 Закрытое Акционерное Общество "Биокад" Высокоаффинные и агрегационно стабильные антитела на основе вариабельных доменов vl и производного vhh
MA41044A (fr) 2014-10-08 2017-08-15 Novartis Ag Compositions et procédés d'utilisation pour une réponse immunitaire accrue et traitement contre le cancer
IL302725A (en) * 2016-01-13 2023-07-01 Regeneron Pharma Rodents having an engineered heavy chain diversity region

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843685A (en) * 1988-09-06 1998-12-01 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP0967277A3 (en) 1988-09-06 2003-10-15 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens
EP0404003A3 (en) 1989-06-19 1991-10-16 Xoma Corporation Chimeric mouse-human km10 antibody with specificity to a human tumor cell antigen
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
JPH07289267A (ja) * 1994-04-25 1995-11-07 Oriental Yeast Co Ltd 発現増強方法
JPH09313191A (ja) 1996-03-28 1997-12-09 Mitsui Petrochem Ind Ltd 蛋白質の分泌生産法
US5824502A (en) * 1996-03-28 1998-10-20 Mitsui Chemicals, Inc. Method for secretory production of protein
AU770952B2 (en) * 1999-03-01 2004-03-11 Genentech Inc. Antibodies for cancer therapy and diagnosis
US7425328B2 (en) * 2003-04-22 2008-09-16 Purdue Pharma L.P. Tissue factor antibodies and uses thereof
DE10325026B4 (de) 2003-06-02 2013-04-18 Genencor International,Inc. Bakterien mit erhöhter Proteinsekretion, Nukleotidsequenzen codierend für ein SecA Protein mit erhöhter Proteinsekretion sowie Verfahren zur Produktion von Proteinen
US20040266993A1 (en) 2003-06-30 2004-12-30 Evans Glen A. Non-immunoglobulin binding polypeptides
JP2007502991A (ja) * 2003-08-20 2007-02-15 バイオサイト インコーポレイテッド 生物活性ナトリウム利尿ペプチドを測定するための、およびその治療可能性を向上させるための方法および組成物
JP4782700B2 (ja) * 2004-01-20 2011-09-28 カロバイオス ファーマシューティカルズ インコーポレイティッド 最低限必須な結合決定基を用いた抗体特異性の移入
US8211648B2 (en) * 2005-07-22 2012-07-03 Kalobios Pharmaceuticals, Inc. Secretion of antibodies without signal peptides from bacteria

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843685A (en) * 1988-09-06 1998-12-01 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Alan I.Derman et al..A signal sequence is not required for protein export in prlA mutants of Escherichia coli.《The EMBO Journal》.1993,第12卷(第3期),参见第879页左列"摘要部分"，第880、881页左列倒数第一、二段
Alan I.Derman et al..A signal sequence is not required for protein export in prlA mutants of Escherichia coli.《The EMBO Journal》.1993,第12卷(第3期),参见第879页左列"摘要部分",第880、881页左列倒数第一、二段,第886页左列"Bacterial strains"部分,右列"Strain constructions"部分. *
ANTON V.ZAVIALOV et al..Secretion of Recombinant Proteins via the Chaperone/Usher pathway in Escherichia coli.《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》.2001,第67卷(第4期),全文. *
第886页左列"Bacterial strains"部分，右列"Strain constructions"部分.

Also Published As

Publication number Publication date
AU2006276823A1 (en) 2007-02-15
IL188889A (en) 2014-09-30
KR20080031452A (ko) 2008-04-08
AU2006276823B2 (en) 2010-04-22
IL188889A0 (en) 2008-04-13
WO2007018853A2 (en) 2007-02-15
CA2615776C (en) 2014-05-13
WO2007018853A3 (en) 2009-04-16
US20070020685A1 (en) 2007-01-25
US9127046B2 (en) 2015-09-08
CA2615776A1 (en) 2007-02-15
EP1907610A4 (en) 2009-12-23
CN101568647A (zh) 2009-10-28
JP2009510998A (ja) 2009-03-19
SG177895A1 (en) 2012-02-28
JP5087541B2 (ja) 2012-12-05
HK1113393A1 (en) 2008-10-03
NZ565126A (en) 2011-03-31
KR101014891B1 (ko) 2011-02-15
US20120309055A1 (en) 2012-12-06
US8211648B2 (en) 2012-07-03
EP1907610B1 (en) 2013-09-11
EP1907610A2 (en) 2008-04-09

Similar Documents

Publication Publication Date Title
US9127046B2 (en) Secretion of antibodies without signal peptides from bacteria
Richard et al. In vivo neutralization of α-cobratoxin with high-affinity llama single-domain antibodies (VHHs) and a VHH-Fc antibody
Robinson et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria
Seixas et al. Antibody-based immunotherapies as a tool for tackling multidrug-resistant bacterial infections
Tsurushita et al. Humanization of a chicken anti-IL-12 monoclonal antibody
TW200848427A (en) Ligand
US20080213224A1 (en) Immunoglobulin against helicobacter pylori
Laffly et al. Selection of a macaque Fab with framework regions like those in humans, high affinity, and ability to neutralize the protective antigen (PA) of Bacillus anthracis by binding to the segment of PA between residues 686 and 694
JP2013520160A (ja) 緑膿菌の血清型ｉ型リポ多糖に対する抗体
Singh et al. Construction of a single-chain variable-fragment antibody against the superantigen staphylococcal enterotoxin B
WO2009033009A2 (en) Antibodies against flagellin and uses thereof
Ayyar et al. Facile domain rearrangement abrogates expression recalcitrance in a rabbit scFv
CA2701047C (fr) Anticorps contre les toxines du charbon
JP2004533264A (ja) バクテリオファージのシグナルペプチドをコードする発現ベクター
Suksomboon et al. Construction, expression, and characterization of scFv fragment against Fasciola gigantica cathepsin L1H
Sepúlveda et al. Binders based on dimerised immunoglobulin VH domains
WO2017186784A1 (en) Autodisplay of antibodies
JP4392375B2 (ja) 人工ポリクローナル免疫グロブリンの製造方法、並びに、免疫グロブリン製剤の製造方法
HK1113393B (en) Secretion of antibodies without signal peptides from bacteria
Silva Exploitation of Burkholderia cenocepacia J2315 surface-exposed proteins as targets for immunotherapeutic approaches
EP2235057A2 (fr) Immunoglobuline g contre les toxines du charbon
Chen et al. SFB flagellin mediates cell adhesion, endocytosis and immune regulation in germ-free mice
Fischer Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit
CN121591884A (zh) CP-IgM抗体及其制备方法和应用、核酸分子、载体、细胞、质控品、试剂盒和CP-IgM抗体的检测方法
JP3908257B2 (ja) ニワトリ型モノクローナル抗体用のオリゴヌクレオチド

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121003

Termination date: 20160707

CF01 Termination of patent right due to non-payment of annual fee