CN101536728A - Method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism-chemical combined detoxification method - Google Patents
Method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism-chemical combined detoxification method Download PDFInfo
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- CN101536728A CN101536728A CN200910081604A CN200910081604A CN101536728A CN 101536728 A CN101536728 A CN 101536728A CN 200910081604 A CN200910081604 A CN 200910081604A CN 200910081604 A CN200910081604 A CN 200910081604A CN 101536728 A CN101536728 A CN 101536728A
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Abstract
The invention discloses a method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism fermentation and chemical treatment, belonging to the field of feed resources. The method comprises the following steps of: adding bran, carbohydrate, ferrous salts, vegetable oil and water, mixing, stirring evenly, sterilizing, then inoculating the culture fluid of lactobacillus planterum and candida tropicalis strain in cotton dregs, controlling temperature and fermenting. The cottonseed protein produced by adopting the method has the content of free gossypol of less than 300PPM; the cottonseed protein generates high-quality protein material containing conjugated linoleic acid, small peptide and amino acid by fermentation treatment of the lactobacillus planterum and candida tropicalis, thus effectively relieving the problem of shortage of protein feed in China.
Description
Technical field
The invention belongs to the feed resource technical field, relate to a kind of microorganism-chemical combined detoxification method preparation and contain the method for CLA high-quality cottonseed protein.
Background technology
China is a cotton big country of product, and at present, there is the cottonseed more than 1,100 ten thousand tons every year in China, and year can be produced cotton cake dregs more than 6,000,000 tons, the stock number whole world first.The accessory substance that cotton cake dregs is a cottonseed after squeezing or extraction deoil processing, the accessory substance after squeezing is a cottonseed cake, the accessory substance after the extraction processing is the cottonseed dregs of rice.Its nutritional labeling is different and different with the shelling degree according to cottonseed quality, processing mode.Its main characteristics: (1) crude protein content height, cotton cake dregs contains protein 38%～50% usually, and to compare amino acid content unbalanced with dregs of beans, and lysine is few, and arginine content is high especially, but the cottonseed dregs of rice are good sources of tryptophan in the feed, methionine.(2) the cotton cake dregs crude fiber content is with shelling and lint degree and difference, and effective energy value also changes thereupon.Usually to compare crude fiber content higher with dregs of beans, is 65% of dregs of beans and can be worth.(3) cotton cake dregs vitamin E and B family vitamin content are abundant, and the few phosphorus of calcium is many.(4) proteinaceous nutrient is amino acid nutrient and small peptide nutrient, and the form (dipeptides and tripeptides) that can absorb peptide with small intestine is that animal supply amino acid has potential nutritional significance for improving the feed protein utilization rate.So,,, can alleviate the problem of China's protein resource anxiety if can be apt to add utilization to it at the nutritional characteristic of cottonseed protein.
A major reason that influences the cottonseed protein utilization is to contain free gossypol in the cotton cake dregs, directly influences the feeding value of cotton cake dregs.Gossypol is many to be present in the cottonseed pigment gland with adipose gland body or gumminess, accounts for 0.7% ~ 4.8% of cottonseed total amount usually.Free gossypol toxicity is bigger, can with enzyme or other combined with protein, destroy its active component, reduce protein digestibility, even make the histoorgan generation pathological change of animal, thereby cause growth of animal slow, poison and dead.
Anticancer, functions such as promotion is grown, inhibition accumulation of fat, enhancing body immunity that CLA has can improve pig, the fowl speed of growth and lean meat percentage, are the research focuses of subjects such as current medicine, health food and Animal nutrition.But because CLA costs an arm and a leg, it is too high to add cost in the feed, uses few in the production practices.Therefore, the animal nutrition and feed scholar is seeking other suitable, cost effective method that improves cla levels in the livestock products.
The method of free gossypol detoxification mainly contains physics, chemistry, microbial fermentation.The present invention organically combines chemical method and microbe fermentation method, and uses the Lactobacillus plantarum that can produce CLA, produces the functional high-quality cottonseed protein that contains CLA, peptide and several amino acids, does not still have report at home.
Summary of the invention
Technical problem to be solved by this invention is: the toxicity that is intended to solve free gossypol in the cottonseed protein, improve the cottonseed protein nutritional labeling, and the method for the high-quality cottonseed protein of CLA is rich in the fermentation preparation that Lactobacillus plantarum is provided, to improve the addition of cottonseed protein in feed, alleviate the problem of protein resource anxiety.
The method that the preparation of this microorganism-chemical combined detoxification method contains the CLA high-quality cottonseed protein is: after earlier candida tropicalis and Lactobacillus plantarum being spread cultivation in batches, after again the cottonseed protein impurity elimination being pulverized, in cottonseed flour, add ferric sulfate, wheat bran, carbohydrate, vegetable fat and water, behind the mixing and stirring sterilization, Lactobacillus plantarum and candida bacterial strain nutrient solution are inoculated in the cottonseed cake (dregs of rice) temperature controlled fermentation.
The present invention will be further described below in conjunction with instantiation
The specific embodiment:
1, culture of strains
1) slant activation is cultivated: potato 100g, add 500ml water boil 30min again moisturizing to 500ml, 10g glucose, 10g agar.28 ℃ of constant temperature culture 48h.
2) seed liquor preparation: get the fresh slant strains of 2 rings and insert seed culture fluid, 28 ℃ of constant temperature shaking tables are cultivated 48h, rotating speed 250r/min.
2, with seed fermentation liquid solid fermentation substrate cultivation base is carried out solid fermentation
Cottonseed protein and wheat bran, corn flour are mixed into basic substrate in 70: 20: 10 ratios, add the 1000-2000PPM molysite, and add vegetable fat, natural pH value, autoclave sterilization in the ratio of 1%-3%.Add seed fermentation liquid when being cooled to 28-30 ℃, inoculum concentration is 3-5%, leaves standstill and cultivates 3 days.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, natural airing to moisture content is lower than 12% back pulverizing and makes finished product.
Claims (5)
1, a kind of microorganism-chemical combined detoxification preparation contains the method for the high-quality cottonseed protein of CLA, it is characterized in that:
1) candida tropicalis (Candida tropicalis) and Lactobacillus plantarum (Lactobacillus plantarum) being carried out batch under certain condition, spreads cultivation.
2) inoculation candida tropicalis and Lactobacillus plantarum in the sterilization cottonseed protein of removing foreign material: back and molysite, wheat bran, corn flour, vegetable fat and water mixing and stirring are pulverized in the cottonseed protein impurity elimination, after sterilizing, spray above-mentioned also diluted candida tropicalis and the Lactobacillus plantarum nutrient solution of spreading cultivation of inoculation, the anaerobic condition bottom fermentation.
2, a kind of microorganism according to claim 1-chemical combined detoxification preparation contains the method for the high-quality cottonseed protein of CLA, it is characterized in that: the substrate proportioning: cottonseed cake (dregs of rice): wheat bran: corn flour=70: 20: 10.
3, a kind of microorganism according to claim 2-chemical combined detoxification preparation contains the method for the high-quality cottonseed protein of CLA, it is characterized in that: described vegetable fat is cottonseed oil, sunflower oil, rapeseed oil or peanut oil, and the vegetable fat addition is 1-3%.
4, a kind of microorganism according to claim 1-chemical combined detoxification preparation contains the method for the high-quality cottonseed protein of CLA, and it is characterized in that: bacterial classification is candida tropicalis and Lactobacillus plantarum.During inoculation, the bacterial classification addition is the 3%-5% of cottonseed protein amount.The microbial fermentation time is 36-60 hour.Fermentation temperature is 28-30 ℃, substrate moisture about 50%.
5, a kind of microorganism according to claim 1-chemical combined detoxification preparation contains the method for the high-quality cottonseed protein of CLA, and it is characterized in that: the dosage of the molysite of interpolation is 1000-2000PPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910081604A CN101536728A (en) | 2009-04-07 | 2009-04-07 | Method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism-chemical combined detoxification method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910081604A CN101536728A (en) | 2009-04-07 | 2009-04-07 | Method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism-chemical combined detoxification method |
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| CN101536728A true CN101536728A (en) | 2009-09-23 |
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| CN200910081604A Pending CN101536728A (en) | 2009-04-07 | 2009-04-07 | Method for preparing high-quality cottonseed protein containing conjugated linoleic acid by microorganism-chemical combined detoxification method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810244A (en) * | 2010-04-29 | 2010-08-25 | 湖南农业大学 | Biological detoxification method of cottonseed meal |
| CN105255793A (en) * | 2015-11-20 | 2016-01-20 | 江南大学 | Lactobacillus plantarum capable of degrading gossypol and application of lactobacillus plantarum |
-
2009
- 2009-04-07 CN CN200910081604A patent/CN101536728A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810244A (en) * | 2010-04-29 | 2010-08-25 | 湖南农业大学 | Biological detoxification method of cottonseed meal |
| CN105255793A (en) * | 2015-11-20 | 2016-01-20 | 江南大学 | Lactobacillus plantarum capable of degrading gossypol and application of lactobacillus plantarum |
| CN105255793B (en) * | 2015-11-20 | 2019-03-01 | 江南大学 | A kind of lactobacillus plantarum and its application with degradation gossypol effect |
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Open date: 20090923 |