CN101502232A - Seedling cultivation method for sea-tangle - Google Patents
Seedling cultivation method for sea-tangle Download PDFInfo
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- CN101502232A CN101502232A CNA2009100145103A CN200910014510A CN101502232A CN 101502232 A CN101502232 A CN 101502232A CN A2009100145103 A CNA2009100145103 A CN A2009100145103A CN 200910014510 A CN200910014510 A CN 200910014510A CN 101502232 A CN101502232 A CN 101502232A
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Abstract
The present invention discloses a seedling cultivation method of sea tangle, wherein the method comprises the following steps: a. cultivating the collected zoospores under the white radiation irradiation with a certain illumination intensity for forming gametophyte; b. cultivating the gametophyte with the blue light illumination with a certain illumination intensity for converting the gametophyte to micro sporinite; c. growing the micro sporinite under the red light illumination with a certain illumination intensity for a certain period of time; and d. cultivating the sporinite under blue light illumination with a certain illumination intensity so that the sporinite obtains the dimension required for stored cargo delivery. The healthy growing of sporinite can be guaranteed under a higher temperature through adopting the combination irradiation with various light qualities for executing seedling cultivation of sea tangle. The method is simple for applying and the seedling cultivation cost is low.
Description
Technical field
The present invention relates to a kind of method of kelp seedling cultivation, specifically, relate to a kind of method of utilizing the combination light quality to carry out kelp seedling cultivation, belong to technical field of aquaculture.
Background technology
Sea-tangle is to carry out one of tangleweed kind of propagating artificially the earliest.According to the historical document record, sea-tangle is not China's native country species, passes to China in the last ability of the '20s in last century by Japan, it is coastal mainly to be distributed in the Dalian at that time, because sea environment is fit to the sea-tangle growth, by 1949, local sea-tangle calendar year output reached 80.6 tons (dry products).But the domestic relatively market demand is still an utterly inadequate amount, mainly also will rely on from Korea and Japanese import.After 1949, China algologist has begun the commercialization culture technique research of sea-tangle.To the fifties, developed natural daylight cryogenic flow rice seedling on watered bed and total man worker's raft culture technology, especially to cultivate summer sporelings (of laminaria) and substitute natural waters cultivation Qiu Miaohou when indoor natural light low temperature, about in advance two months of sea tangle seedling distributing time, the unit cultured output is greatly improved.At present, laminaria culture scale and algae culturing first place, the output Jun Ju world wherein derive from China again more than 80%.2006,243.9 hundred million strains of China's kelp seedling cultivation, 4.1 ten thousand hectares of cultured areas, about 850,000 tons of cultured output, about 55.25 hundred million yuan of the wound gross output value is occupied in mariculture and consequence.
Be typical heterogenesis the history of life of sea-tangle: the sporophyte maturation forms sporangium later on, and the trip spore diffuses the back and sprouts the formation female and male gametophytes, and female and male gametophytes discharges ovum and sperm more respectively, and smart ovum becomes the kelp spore body in conjunction with the back merisis.Grow and the envirment factor in the sea-tangle history of life in each stage are closely related.Studies show that the ripe area of sporangium is big in the time of 18-22 ℃, the maturity height, the spore amount of diffusing is big, and swimming and sprout energetic; And the suitable water temperature that sporophyte is nourished and grown is lower, is 1-13 ℃, and tenability limit is generally about 19.5 ℃.
The laminaria culture process mainly comprises two stages: grow seedlings the stage with become the algae cultivation stage.According to the characteristics history of life of sea-tangle, in the north, sea-tangle adopt the spore time generally in 7 months, the last ten-days period (water temperature is lower than 22 ℃), the trip spore of collection carries out low temperature under indoor natural light grows seedlings, and cultivates into sporophyte; The sporophyte that reaches the outbound requirement to by the end of October (water temperature is reduced to below 19.5 ℃) just culture by the indoor sea of transferring to.Indoor natural light low temperature grow seedlings for up to 3 months, between nursery stage, want temperature and illumination in the strict control room, otherwise green rot disease, white mashed sick and fall disease such as seedling take place easily.Some nurseries are attempted with chemicals disease being treated, but do not obtain good result, still take to reduce at present temperature basically and carry out the control of disease with adjusting illumination.But there is following shortcoming in this traditional seedling-cultivating method: on the one hand, take place for preventing disease, the water temperature between the whole nursery stage of sporophyte need be controlled at below 9 ℃, and lower more than 10 ℃ than marine natural temperature, the cost that is used for refrigeration cool-down is very high.On the other hand,, want temperature and illumination in the strict control room, sporophyte is slowly grown, prevent that disease from taking place in the mid-term of growing seedlings because the indoor raising of seedling time is longer; And growing seedlings the later stage, and need to regulate temperature and illumination, make sporophyte grow fast, to reach the outbound dimensional requirement.Because the illumination condition of natural daylight has the variation of diurnal periodicity, be subjected to the influence of weather condition simultaneously again, need take different measure in time to regulate in the seedling raising process, also be difficult to control sometimes, waste time and energy very much.
Summary of the invention
The present invention is directed to the problems referred to above and the defective that exist during sea-tangle natural daylight low temperature is grown seedlings in the prior art, a kind of seedling cultivation method for sea-tangle is provided, has carried out kelp seedling cultivation, can under higher temperature, guarantee the healthy growth of sporophyte by the combination irradiation that adopts light quality, method is simple, and cost is lower.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
A kind of seedling cultivation method for sea-tangle is characterized in that, comprises the steps:
A, the trip spore that will gather are cultivated certain hour under the white light of certain intensity of illumination, form gametophyte;
The blue light illumination of b, the certain intensity of illumination of employing is cultivated above-mentioned gametophyte, makes gametophyte change into microsporinite;
C, with the above-mentioned microsporinite certain hour of under the red light irradiation of certain intensity of illumination, growing;
D, cultivate sporophyte with the blue light illumination of certain intensity of illumination again, make sporophyte reach the size of outbound requirement.
According to the present invention, for guaranteeing the normal growth and the growth in each stage of sea-tangle in the seedling raising process, the intensity of illumination of white light is 800-15001x among the described step a, and it is too fast to guarantee that sea-tangle neither can grow, and also can not have influence on the growth of later stage kelp gametophyte to sporophyte; The intensity of illumination of blue light is 2000-30001x in described step b and the steps d; The intensity of illumination of ruddiness is 1500-20001x among the described step c.
Preferably, the intensity of illumination of described blue light is 2500-30001x.
According to the present invention, the time of white light is 12-18 days among the described step a; And the requirement of sporophyte size is determined when the irradiation time of blue light and ruddiness is according to the length of growing-seedling period and outbound in described step b, step c and the steps d.
According to the present invention, when adopting said method to carry out kelp seedling cultivation, the water temperature of growing seedlings is one 13 ℃ of youngsters.
Compared with prior art, advantage of the present invention and good effect are: utilize the combination of different light medium such as white light, blue light and ruddiness to carry out the sea-tangle indoor raising of seedling, can exceed traditional seedling-cultivating method at least under 2-4 ℃ the situation in water temperature, guarantee the healthy growth of sea-tangle, and when outbound, reach the standard that seawater is cultured, thereby reduced expensive because of what the water temperature refrigeration cool-down of growing seedlings was produced; In addition, the intensity of illumination of various light qualities is selected and control easily, has avoided changing in traditional seedling raising process the difficulty and the loaded down with trivial details work of natural lighting condition, has improved the simplification of kelp seedling cultivation process.
Description of drawings
Fig. 1 is the schematic layout pattern of a used white led lamps of embodiment of seedling cultivation method for sea-tangle of the present invention;
Fig. 2 is the blue led lamp used among the described embodiment of Fig. 1 and the schematic layout pattern of red LED lamp;
Fig. 3 is the growing state microscopical examination showed intention of kelp spore body among the described embodiment of Fig. 1;
Wherein, a is that sea-tangle fertilized egg is cultivated the microsporinite that forms after 18 days under blue light, b is that the microsporinite among a is transferred to the growing state of cultivating under the ruddiness after 45 days, c is that the microsporinite among the b is transferred under the blue light growing state of cultivating after 18 days, and d is the microsporinite of apposition growth on the coir rope after mixing light quality and growing seedlings 96 days.
Embodiment
The present invention is further detailed explanation below in conjunction with accompanying drawing.
Experimental study shows, different light medium develops into the difference that influences of the growth of microsporinite, microsporinite and microsporinite disease control etc. to kelp gametophyte in the kelp seedling cultivation process, and is specific as follows:
Kelp gametophyte is developed into the influence of microsporinite: compare with white light, the time that blue light impels gametophyte to develop into microsporinite shifts to an earlier date, and ruddiness postpones the time that gametophyte develops into microsporinite, and under the ruddiness, have only the egagametophyte ovulation bud into microsporinite about 45%, other egagametophytes are along with the prolongation of incubation time develops to filamentous.
Influence to the growth of sea-tangle microsporinite: the growth rate of sea-tangle microsporinite is the fastest under the blue light, and growth rate is very slow under the ruddiness.Influence to the disease control of sea-tangle microsporinite: under blue light and red light irradiation condition, the length of sea-tangle microsporinite constantly increases, and form, color are all normal.And when white light, initial stage microsporinite form is normal; Along with incubation time lengthening, plaquelike meronecrosis appears in the microsporinite top, i.e. green rot disease, and then slightly down from the middle rib to the leaf margin, white point one by one appears in the cell death of turning white, and promptly point-like is mashed in vain sick; Along with incubation time further extends, rotting of microsporinite top occurrence of large-area causes the length of microsporinite to shorten.
Because the sea-tangle indoor raising of seedling need reach time about 3 months, and require in whole seedling raising process that sporophyte can not grow too fast, avoiding the generation of disease, and when outbound, grow into the size of outbound requirement again.Therefore, the present invention is according to white light, blue light and the ruddiness Different Effects to each vegetative stage of sea-tangle, indoor raising of seedling is divided into four-stage: the phase I adopts white light to cultivate, it is comparatively slow both to have guaranteed that sea-tangle trip spore grew, and don't influences the growth of later stage kelp gametophyte to sporophyte; After the trip spore is cultivated a period of time, enter the second stage that blue light is cultivated, make gametophyte synchronous relatively, thereby obtain the more consistent microsporinite of size to the conversion of sporophyte; Phase III, in the microsporinite process of growth, cultivate with ruddiness earlier, make microsporinite grow lentamente; And when being about to outbound, adopt blue light illumination again, accelerate the growth of sporophyte, thereby obtain health, satisfactory sea-tangle seedling.Describe the process of seedling cultivation method for sea-tangle of the present invention below in detail with the form of embodiment.
Embodiment one: described embodiment kelp seedling cultivation carries out in water tank, and water temperature is controlled at youngster ℃ in the water tank.Water tank bottom evenly is equipped with coir rope, and immersible pump is placed in the water tank the inside, makes that the water body in the water tank flows.Be mounted with white led lamps, blue led lamp and red LED lamp on the water tank top, respectively as the light source of white light, blue light and ruddiness.Wherein, the schematic layout pattern of white led lamps as shown in Figure 1, every group has 5 LED lamps.The schematic layout pattern of blue led lamp and red LED lamp as shown in Figure 2, every group has 9 LED lamps, the photoperiod of each light quality is 8h illumination (L)/16h dark (D).Whole nursery stage comprises following four-stage:
A1, gathering the trip spore, is that the white light of 8001x was cultivated 15 days with illumination intensity earlier, and trip sporulation gametophyte may have a spot of egagametophyte ovulation formation microsporinite this moment.
B1, to adopt intensity of illumination then be that the blue light of 25001x is cultivated, and cultivated 6 days, and the egagametophyte ovulation more than 95% forms microsporinite; Continuation was cultivated 12 days with blue light again, and the average-size of microsporinite is long 456um, wide 98um, and shown in a figure among Fig. 3, the figure medium scale is 1:100um.
C1, the growth for slowing down microsporinite, the generation that reduces disease after blue light is cultivated 18 days, use ruddiness instead to cultivate, and the ruddiness intensity of illumination is 15001x.Cultivated 45 days with ruddiness, microsporinite is slowly grown, and average-size is long 3.5mm, wide 0.45mm, and shown in b figure among Fig. 3, the figure medium scale is 1:0.5mm.
D1, grow fast,, adopt blue light to cultivate at last again to reach outbound to the desired size of mariculture for making microsporinite.The blue light intensity of illumination still is 25001x, cultivates after 18 days, and it is 25mm, the wide 3mm of being that microsporinite grows into average length fast, reaches the size of outbound requirement, and shown in c figure and d figure among Fig. 3, wherein, the c drawing scale is 1:15mm, and the d drawing scale is 1:30mm.
In above-mentioned 96 days by a definite date indoor high temperature (exceeding at least 2 ℃ than conventional method) incubation, the microsporinite growth conditions is good, white mashed disease, green rot disease can not occur and fall diseases such as seedling.
Embodiment two: this embodiment device therefor is identical with embodiment one, and difference is that water temperature is controlled at 13 ℃ in this embodiment water tank.
Whole nursery stage comprises following four-stage:
A2, gathering the trip spore, is that the white light of 15001x was cultivated 12 days with illumination intensity earlier, and trip sporulation gametophyte may have a spot of egagametophyte ovulation formation microsporinite this moment.
B2, to adopt intensity of illumination then be that the blue light of 30001x is cultivated, and cultivated 4 days, and the egagametophyte ovulation more than 95% forms microsporinite; Continuation was cultivated 11 days with blue light again, and the average-size of microsporinite is long 456um, wide 98um.
C2, the growth for slowing down microsporinite, the generation that reduces disease after cultivating 15 days with blue light, use ruddiness instead to cultivate, and the ruddiness intensity of illumination is 20001x.Cultivated 35 days with ruddiness, microsporinite is slowly grown, and average-size is long 4mm, wide 0.5mm.
D2, grow fast,, adopt blue light to cultivate at last again to reach outbound to the desired size of mariculture for making microsporinite.The blue light intensity of illumination still is 30001x, cultivates after 18 days, and microsporinite grows into long be 35mm, the wide 4mm of being fast, reaches the size of outbound requirement.
In above-mentioned 80 days by a definite date indoor high temperature (exceeding at least 4 ℃ than conventional method) incubation, the microsporinite growth conditions is good, significantly white mashed sick, green rot disease occurs and falls disease such as seedling.
When in the above-described embodiments, the irradiation time of blue light and ruddiness can be according to the length of actual growing-seedling period and outbound in each stage the actual requirement of sea-tangle seedling sporophyte size is adjusted.
Certainly; the above only is a kind of preferred implementation of the present invention; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1, a kind of seedling cultivation method for sea-tangle is characterized in that, comprises the steps:
A, the trip spore that will gather are cultivated certain hour under the white light of certain intensity of illumination, form gametophyte;
The blue light illumination of b, the certain intensity of illumination of employing is cultivated above-mentioned gametophyte, makes gametophyte change into microsporinite;
C, with the above-mentioned microsporinite certain hour of under the red light irradiation of certain intensity of illumination, growing;
D, cultivate sporophyte with the blue light illumination of certain intensity of illumination again, make sporophyte reach the size of outbound requirement.
2, seedling cultivation method for sea-tangle according to claim 1 is characterized in that, the intensity of illumination of white light is 800-15001x among the described step a.
3, seedling cultivation method for sea-tangle according to claim 1 is characterized in that, the intensity of illumination of blue light is 2000-30001x in described step b and the steps d.
4, seedling cultivation method for sea-tangle according to claim 3 is characterized in that, the intensity of illumination of described blue light is 2500-30001x.
5, seedling cultivation method for sea-tangle according to claim 1 is characterized in that, the intensity of illumination of ruddiness is 1500-20001x among the described step c.
According to each described seedling cultivation method for sea-tangle in the claim 1 to 5, it is characterized in that 6, the time of white light is 12-18 days among the described step a.
7, seedling cultivation method for sea-tangle according to claim 6 is characterized in that, when the irradiation time of blue light and ruddiness is according to growing-seedling period and outbound in described step b, step c and the steps d requirement of sporophyte size is determined.
8, seedling cultivation method for sea-tangle according to claim 1 is characterized in that, the water temperature of kelp seedling cultivation is 11-13 ℃.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771394A (en) * | 2012-07-27 | 2012-11-14 | 山东东方海洋科技股份有限公司 | Method for cloning and culturing seaweed gametophytes |
| CN105766613A (en) * | 2016-01-08 | 2016-07-20 | 山东省海洋生物研究院 | Breeding method of kelp early-maturing variety |
| CN106718819A (en) * | 2017-01-06 | 2017-05-31 | 山东大学 | The prevention method of the white rotten disease of sea tangle sporophyte |
| CN110591999A (en) * | 2019-08-30 | 2019-12-20 | 中国水产科学研究院黄海水产研究所 | Nutrient propagation method for clone line of laminaria japonica aresch gametophyte |
| CN111699968A (en) * | 2020-07-10 | 2020-09-25 | 中国海洋大学 | Method for preventing and treating kelp albinism |
| CN112970576A (en) * | 2021-03-16 | 2021-06-18 | 烟台市海洋经济研究院 | Breeding method of strong light-resistant kelp variety |
| CN114503908A (en) * | 2022-03-13 | 2022-05-17 | 威海长青海洋科技股份有限公司 | Kelp seedling culture method for controlling zoospore diffusion through accurate counting |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148115C (en) * | 2000-09-08 | 2004-05-05 | 中国海洋大学 | Gametophyte cloning method of breeding heterotic kelp |
| CN1302703C (en) * | 2004-06-24 | 2007-03-07 | 山东东方海洋科技股份有限公司 | Sea weeds seedling cultivation process using deep well seawater |
| CN100340159C (en) * | 2005-08-04 | 2007-10-03 | 山东东方海洋科技股份有限公司 | Programmed freezing preservation method of kelp clone |
| CN1736163A (en) * | 2005-09-01 | 2006-02-22 | 烟台大学 | Kelp variety optimization production process |
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2009
- 2009-02-26 CN CN2009100145103A patent/CN101502232B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102771394A (en) * | 2012-07-27 | 2012-11-14 | 山东东方海洋科技股份有限公司 | Method for cloning and culturing seaweed gametophytes |
| CN105766613A (en) * | 2016-01-08 | 2016-07-20 | 山东省海洋生物研究院 | Breeding method of kelp early-maturing variety |
| CN106718819A (en) * | 2017-01-06 | 2017-05-31 | 山东大学 | The prevention method of the white rotten disease of sea tangle sporophyte |
| CN106718819B (en) * | 2017-01-06 | 2019-07-30 | 山东大学 | The prevention method of the white rotten disease of sea tangle sporophyte |
| CN110591999A (en) * | 2019-08-30 | 2019-12-20 | 中国水产科学研究院黄海水产研究所 | Nutrient propagation method for clone line of laminaria japonica aresch gametophyte |
| CN110591999B (en) * | 2019-08-30 | 2022-03-01 | 中国水产科学研究院黄海水产研究所 | Nutrient propagation method for clone line of laminaria japonica aresch gametophyte |
| CN111699968A (en) * | 2020-07-10 | 2020-09-25 | 中国海洋大学 | Method for preventing and treating kelp albinism |
| CN111699968B (en) * | 2020-07-10 | 2022-03-04 | 中国海洋大学 | Method for preventing and treating kelp albinism |
| CN112970576A (en) * | 2021-03-16 | 2021-06-18 | 烟台市海洋经济研究院 | Breeding method of strong light-resistant kelp variety |
| CN114503908A (en) * | 2022-03-13 | 2022-05-17 | 威海长青海洋科技股份有限公司 | Kelp seedling culture method for controlling zoospore diffusion through accurate counting |
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