CN101416644A - A kind of walnut peel extract and new purposes thereof - Google Patents
A kind of walnut peel extract and new purposes thereof Download PDFInfo
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- CN101416644A CN101416644A CNA2008100565158A CN200810056515A CN101416644A CN 101416644 A CN101416644 A CN 101416644A CN A2008100565158 A CNA2008100565158 A CN A2008100565158A CN 200810056515 A CN200810056515 A CN 200810056515A CN 101416644 A CN101416644 A CN 101416644A
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Abstract
The present invention discloses a kind of walnut peel extract and preparation method thereof; The object of the invention also is to provide the new purposes of sterilization of walnut peel extract.
Description
Technical field
The present invention relates to a kind of plant extracts and preparation method thereof, particularly a kind of walnut peel extract and preparation method thereof and new purposes.
Background technology
Walnut (Juglans regia) is a Juglandaceae Juglans deciduous tree, has the cultivation history in more than 2,000 year.People found black walnut tree down other plant such as apple, pine, weeds etc. can not grow, and other trees are weedy down.Walnut Resources in China is abundant, is distributed widely in China each department.
At present, to the research of green peel of walnut mainly based on medicine.The thick green pericarp of one deck of outside when green peel of walnut is the walnut prematurity along with the blackening gradually of the ripe gradually Chinese olive skin of walnut, comes off at last naturally.It is a kind of noxious material, and normal person's overdose can cause death, but has the people of cancer to take and can cure the disease.According to the steep in wine effect of treatment stomach trouble of the rascal walnut of plucking before pharmacopeia record first fu, clinical and among the people have eat the example that the Chinese olive skin is used for treating cancer.Green peel of walnut suffering, hardship, puckery, poisonous, be slightly cold, have heat-clearing, detoxifcation, end dysentery, make eye bright, antineoplastic action, can control have loose bowels, dysentery, leukorrhea, hot eyes etc.Botanical fungicide is that a class utilization contains some part of Fungicidal active substance or the bactericide that effective component extracting is made, have efficient, low toxicity or nontoxic, pollution-free, selectivity is high, do not make advantages such as insect develops immunity to drugs, meet the development trend that agricultural chemicals transforms to " the harmonious agricultural chemicals (Enviroment acceptable pesticide) of environment " or " biorational agricultural chemicals (biorationalpesiticide) " from traditional organic chemicals.At present, the research and development of botanical fungicide is become the innovative point of new type bactericide.
Summary of the invention
The object of the invention is to provide walnut peel extract; Another purpose of the present invention be to provide walnut peel extract the preparation method; The object of the invention also is to provide the new purposes of sterilization of walnut peel extract.
The present invention seeks to be achieved through the following technical solutions:
Extract of the present invention is made by following method:
Take by weighing green peel of walnut powder 60-70 weight portions, add the benzinum of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into 28-35 parts by volume under 40 ℃, ligroin extraction.
Method for preparing extractive of the present invention is preferably:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the benzinum of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, ligroin extraction.
Solvent benzinum in the said method can also be replaced with chloroform, ethyl acetate, methyl alcohol or pure water and obtain chloroform extract, ethyl acetate extract, methanolic extract or water extract respectively.
The relation of described weight portion of foregoing invention and parts by volume is: g/ml.
Description of drawings:
Fig. 1: ligroin extraction is to the inhibitory action of different germs
Fig. 2: chloroform extract is to the inhibitory action of different germs
Fig. 3: ethyl acetate extract is to the inhibitory action of different germs
Fig. 4: methanolic extract is to the inhibitory action of different germs
Fig. 5: water extract is to the inhibitory action of different germs
Following experimental example or embodiment are used for further specifying but are not limited to the present invention.
Experimental example 1 indoor antibacterial test
Adopt growth rate method. Under aseptic condition, get the green peel of walnut for preparing and extract concentrate 1mL, Join in the culture dish that diameter is 9cm, add again the PDA culture medium (about 40 ℃) for preparing 14mL makes the abundant mixing of extract and culture medium, and poisonous culture medium flat plate is made in cooling, connects respectively afterwards Enter for examination bacterium cake (d=4mm), each bacterial classification is established 3 repetitions. Prepare respective amount with same method Extract the solvent culture medium flat plate in contrast. Each bacterium colony is measured by the right-angled intersection method, with its mean value generation The size of table bacterium colony. The computing formula of bacteriostasis rate is as follows: pure increment=bacterium colony average diameter-bacterium cake diameter; Bacteriostasis rate=[(contrast pure increment-process pure increment)/contrast pure increment] * 100%.
Table 1 green peel of walnut different solvents extract is measured the bacteriostasis of various pathogenic bacteria
Tab.1 Antifungal effect mensuration of the different solvents extracts from Walnut Peel
against different hytopathogenic fungi
A. Botryosphaeria berengeriana f. sp Physalospora nasei; B. Valsa mali Valsa mali; C. Monilinia fructicola Monilinia fiucticola; D. Alternaria mali Alternaria alternata; E. eggplant eggplant rot bacterium Fusarium oxysporum; F. fusarium graminearum Botrytis cinerea.
Experimental result: as can be seen from Table 1, to the inhibition of Botryosphaeria berengeriana f. sp best be water extraction Get thing, inhibiting rate reaches 96.88%; The green peel of walnut water extract to the inhibition of Valsa mali Good, inhibiting rate is 100%; The inhibition of chloroform extract is not obvious, and inhibiting rate is 36.78%; Nuclear Peach rascal ligroin extraction and ethyl acetate extract are poor to the inhibition of Monilinia fructicola, and other are 4 years old It is apparent in view to the inhibition to Monilinia fructicola to plant extract, and the inhibition of water extract is strong, Inhibiting rate reaches 100%; The highest to the Alternaria mali water extract, bacteriostasis rate reaches 100%, The inhibition of ethyl acetate and methanolic extract is comparatively obvious, the inhibition effect of benzinum and chloroform extract Fruit is poor; Inhibition to eggplant eggplant rot bacterium water extract is best, and inhibiting rate is 79.14%, acetic acid second Ester extract inhibiting rate is 30.54%, and other 3 kinds of extracts all have stimulation growth, oil to eggplant The spread effect of ether extract is particularly outstanding, and inhibiting rate is 41.63%; Inhibition effect to fusarium graminearum What fruit was best is water extract, and inhibiting rate is 81.31%, and other 4 kinds of extracts also have inhibition.
Following embodiment all can realize the effect of above-mentioned experimental example
Embodiment
Embodiment 1: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 64kg, add the benzinum of 4 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than under 40 ℃ in temperature and is concentrated into 32L, ligroin extraction.
Embodiment 2: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 68kg, add the chloroform of 5 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than under 40 ℃ in temperature and is concentrated into 30L, chloroform extract.
Experimental example 3: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 70kg, add the methyl alcohol of 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, methanolic extract.
Experimental example 4: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 60kg, add the water of 8 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, water extract.
Experimental example 5: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 60kg, add the ethyl acetate of 8 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, ethyl acetate extract.
Claims (11)
1, a kind of walnut peel extract is characterized in that this walnut peel extract made by following method:
Take by weighing green peel of walnut powder 60-70 weight portions, add the benzinum of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into 28-35 parts by volume under 40 ℃, ligroin extraction.
2, walnut peel extract as claimed in claim 1 is characterized in that this walnut peel extract made by following method:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the benzinum of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, ligroin extraction.
3, walnut peel extract as claimed in claim 1 or 2, the benzinum that it is characterized in that making in this walnut peel extract method obtains chloroform extract, ethyl acetate extract, methanolic extract or water extract respectively with chloroform, ethyl acetate, methyl alcohol or pure water replacement.
4, a kind of preparation method of walnut peel extract is characterized in that this method is:
Take by weighing green peel of walnut powder 60-70 weight portions, add the benzinum of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into 28-35 parts by volume under 40 ℃, ligroin extraction.
5, the preparation method of walnut peel extract as claimed in claim 4 is characterized in that this method is:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the benzinum of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, ligroin extraction.
6,, it is characterized in that the benzinum in the method obtains chloroform extract, ethyl acetate extract, methanolic extract or water extract respectively with chloroform, ethyl acetate, methyl alcohol or pure water replacement as the preparation method of claim 4 or 5 described walnut peel extracts.
7, the application of green peel of walnut oil fan extract as claimed in claim 1 or 2 in the bactericidal agent of preparation inhibition eggplant eggplant rot bacterium or inhibition fusarium graminearum.
8, the application of green peel of walnut water extract as claimed in claim 7 in preparing the bactericidal agent that suppresses ring rot of apple bacterium, inhibition Monilinia fructicola, inhibition alternaria leaf spot of apple bacterium, inhibition eggplant eggplant rot bacterium, inhibition fusarium graminearum or suppress Valsa mali.
9, the application of green peel of walnut ethyl acetate extract as claimed in claim 7 in the bactericidal agent of preparation inhibition alternaria leaf spot of apple bacterium, inhibition fusarium graminearum or inhibition eggplant eggplant rot bacterium.
10, the application of green peel of walnut methanolic extract as claimed in claim 7 in the bactericidal agent of preparation inhibition alternaria leaf spot of apple bacterium, inhibition alternaria leaf spot of apple bacterium or inhibition fusarium graminearum.
11, the application of green peel of walnut chloroform extract as claimed in claim 7 in the bactericidal agent of preparation inhibition fusarium graminearum.
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| CN200810056515A CN101416644B (en) | 2008-01-21 | 2008-01-21 | Walnut peel extract and new use thereof |
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| CN200810056515A CN101416644B (en) | 2008-01-21 | 2008-01-21 | Walnut peel extract and new use thereof |
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| CN101416644B CN101416644B (en) | 2010-05-12 |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618066B (en) * | 2009-08-04 | 2011-09-28 | 天津科技大学 | Preparation method of walnut green husk total tannin and application of same |
| CN103222486A (en) * | 2013-05-23 | 2013-07-31 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
| CN103461470A (en) * | 2013-09-03 | 2013-12-25 | 新疆农业科学院农产品贮藏加工研究所 | Walnut green seedcase extract compound preservative as well as preparation method and application thereof |
| CN103766409A (en) * | 2012-10-18 | 2014-05-07 | 王涵 | Walnut green seedcase extract product and bacteriostatic use thereof |
| CN104872207A (en) * | 2015-05-11 | 2015-09-02 | 刘全 | Insecticide bactericide for plants, and preparation method of insecticide bactericide |
| CN104872191A (en) * | 2015-06-16 | 2015-09-02 | 王鲜花 | Drug for curing gummy stem blight and manufacturing method thereof |
| CN104872195A (en) * | 2015-04-16 | 2015-09-02 | 甘肃省分析测试中心 | Green walnut epicarp extract, extracting method and application of extract, and seed coating agent prepared from extract |
| CN105851064A (en) * | 2016-04-06 | 2016-08-17 | 陕西省烟草公司商洛市公司 | Preparation method of walnut green husk extracts for preventing and treating tobacco viral disease |
| CN106490001A (en) * | 2015-09-06 | 2017-03-15 | 新疆农业科学院海南三亚农作物育种试验中心 | A kind of thermal fog and its application with walnut peel extract as effective ingredient |
| CN106614763A (en) * | 2016-11-15 | 2017-05-10 | 济南华鲁食品有限公司 | Method for preparing green insecticide from walnut green seedcase |
| CN107821447A (en) * | 2017-11-30 | 2018-03-23 | 孙铭岐 | A kind of walnut peel extract bactericide and its application process in preventing and treating Fusarium solani |
| CN108409410A (en) * | 2018-03-23 | 2018-08-17 | 山西省农业科学院农业环境与资源研究所 | A kind of antibacterial liquid and preparation method thereof for fruit tree rejuvenation greening |
| CN109111300A (en) * | 2018-11-06 | 2019-01-01 | 山东省葡萄研究院 | A kind of grape special nutritional bacteriostatic agent and the preparation method and application thereof |
| CN109452327A (en) * | 2018-12-18 | 2019-03-12 | 贵州省核桃研究所 | A kind of soil sterilants and its control method for preventing and treating early blight of tomato |
| CN110721570A (en) * | 2019-10-28 | 2020-01-24 | 北京城市排水集团有限责任公司 | Chinese herbal medicine deodorization composition and preparation method thereof |
| CN115226734A (en) * | 2022-08-25 | 2022-10-25 | 渭南职业技术学院 | Walnut green seedcase and Chinese cabbage composite agricultural enzyme composition as well as preparation method and application thereof |
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| CN1314327C (en) * | 2004-12-03 | 2007-05-09 | 武汉绿世纪生物工程有限责任公司 | Use of walnut plant extract as pesticide in preventing and controlling diamondback moth |
| CN101099495B (en) * | 2007-07-27 | 2010-12-08 | 浙江林学院 | Hickory nut husk disinfectant and its producing method |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101618066B (en) * | 2009-08-04 | 2011-09-28 | 天津科技大学 | Preparation method of walnut green husk total tannin and application of same |
| CN103766409A (en) * | 2012-10-18 | 2014-05-07 | 王涵 | Walnut green seedcase extract product and bacteriostatic use thereof |
| CN103222486A (en) * | 2013-05-23 | 2013-07-31 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
| CN103222486B (en) * | 2013-05-23 | 2014-11-19 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
| CN103461470A (en) * | 2013-09-03 | 2013-12-25 | 新疆农业科学院农产品贮藏加工研究所 | Walnut green seedcase extract compound preservative as well as preparation method and application thereof |
| CN104872195A (en) * | 2015-04-16 | 2015-09-02 | 甘肃省分析测试中心 | Green walnut epicarp extract, extracting method and application of extract, and seed coating agent prepared from extract |
| CN104872207A (en) * | 2015-05-11 | 2015-09-02 | 刘全 | Insecticide bactericide for plants, and preparation method of insecticide bactericide |
| CN104872191A (en) * | 2015-06-16 | 2015-09-02 | 王鲜花 | Drug for curing gummy stem blight and manufacturing method thereof |
| CN106490001B (en) * | 2015-09-06 | 2019-01-22 | 新疆农业科学院海南三亚农作物育种试验中心 | It is a kind of using walnut peel extract as the thermal fog of effective component and its application |
| CN106490001A (en) * | 2015-09-06 | 2017-03-15 | 新疆农业科学院海南三亚农作物育种试验中心 | A kind of thermal fog and its application with walnut peel extract as effective ingredient |
| CN105851064A (en) * | 2016-04-06 | 2016-08-17 | 陕西省烟草公司商洛市公司 | Preparation method of walnut green husk extracts for preventing and treating tobacco viral disease |
| CN106614763A (en) * | 2016-11-15 | 2017-05-10 | 济南华鲁食品有限公司 | Method for preparing green insecticide from walnut green seedcase |
| CN107821447A (en) * | 2017-11-30 | 2018-03-23 | 孙铭岐 | A kind of walnut peel extract bactericide and its application process in preventing and treating Fusarium solani |
| CN108409410A (en) * | 2018-03-23 | 2018-08-17 | 山西省农业科学院农业环境与资源研究所 | A kind of antibacterial liquid and preparation method thereof for fruit tree rejuvenation greening |
| CN109111300A (en) * | 2018-11-06 | 2019-01-01 | 山东省葡萄研究院 | A kind of grape special nutritional bacteriostatic agent and the preparation method and application thereof |
| CN109452327A (en) * | 2018-12-18 | 2019-03-12 | 贵州省核桃研究所 | A kind of soil sterilants and its control method for preventing and treating early blight of tomato |
| CN110721570A (en) * | 2019-10-28 | 2020-01-24 | 北京城市排水集团有限责任公司 | Chinese herbal medicine deodorization composition and preparation method thereof |
| CN115226734A (en) * | 2022-08-25 | 2022-10-25 | 渭南职业技术学院 | Walnut green seedcase and Chinese cabbage composite agricultural enzyme composition as well as preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101416644B (en) | 2010-05-12 |
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