CN101416644B - Walnut peel extract and new use thereof - Google Patents
Walnut peel extract and new use thereof Download PDFInfo
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- CN101416644B CN101416644B CN200810056515A CN200810056515A CN101416644B CN 101416644 B CN101416644 B CN 101416644B CN 200810056515 A CN200810056515 A CN 200810056515A CN 200810056515 A CN200810056515 A CN 200810056515A CN 101416644 B CN101416644 B CN 101416644B
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Abstract
The present invention disclose a extract from green peel of walnut and preparation method thereof, and provides novel a sterilization use of the extract from green peel of walnut.
Description
Technical field
The present invention relates to a kind of plant extracts and preparation method thereof, particularly a kind of walnut peel extract and preparation method thereof and new purposes.
Background technology
Walnut (Juglans regia) is a Juglandaceae Juglans deciduous tree, has the cultivation history in more than 2,000 year.People found black walnut tree down other plant such as apple, pine, weeds etc. can not grow, and other trees are weedy down.Walnut Resources in China is abundant, is distributed widely in China each department.
At present, to the research of green peel of walnut mainly based on medicine.The thick green pericarp of one deck of outside when green peel of walnut is the walnut prematurity along with the blackening gradually of the ripe gradually Chinese olive skin of walnut, comes off at last naturally.It is a kind of noxious material, and normal person's overdose can cause death, but has the people of cancer to take and can cure the disease.According to the steep in wine effect of treatment stomach trouble of the rascal walnut of plucking before pharmacopeia record first fu, clinical and among the people have eat the example that the Chinese olive skin is used for treating cancer.Green peel of walnut suffering, hardship, puckery, poisonous, be slightly cold, have heat-clearing, detoxifcation, end dysentery, make eye bright, antineoplastic action, can control have loose bowels, dysentery, leukorrhea, hot eyes etc.Botanical fungicide is that a class utilization contains some part of Fungicidal active substance or the bactericide that effective component extracting is made, have efficient, low toxicity or nontoxic, pollution-free, selectivity is high, do not make advantages such as insect develops immunity to drugs, meet the development trend that agricultural chemicals transforms to " the harmonious agricultural chemicals (Enviroment acceptable pesticide) of environment " or " biorational agricultural chemicals (biorationalpesiticide) " from traditional organic chemicals.At present, the research and development of botanical fungicide is become the innovative point of new type bactericide.
Summary of the invention
The object of the invention is to provide walnut peel extract; Another purpose of the present invention be to provide walnut peel extract the preparation method; The object of the invention also is to provide the new purposes of sterilization of walnut peel extract.
The present invention seeks to be achieved through the following technical solutions:
Extract of the present invention is made by following method:
Take by weighing green peel of walnut powder 60-70 weight portion, add the benzinum of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into the 28-35 parts by volume under 40 ℃, ligroin extraction.
Method for preparing extractive of the present invention is preferably:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the benzinum of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, ligroin extraction.
Solvent benzinum in the said method can also be replaced with chloroform, ethyl acetate, methyl alcohol or pure water and obtain chloroform extract, ethyl acetate extract, methanolic extract or water extract respectively.
The relation of described weight portion of foregoing invention and parts by volume is: g/ml.
Description of drawings:
Fig. 1: ligroin extraction is to the inhibitory action of different germs
Fig. 2: chloroform extract is to the inhibitory action of different germs
Fig. 3: ethyl acetate extract is to the inhibitory action of different germs
Fig. 4: methanolic extract is to the inhibitory action of different germs
Fig. 5: water extract is to the inhibitory action of different germs
Following experimental example or embodiment are used to further specify but are not limited to the present invention.
Experimental example 1 indoor antibacterial test
Adopt growth rate method.Under aseptic condition, get the green peel of walnut for preparing and extract concentrate 1mL, join in the culture dish that diameter is 9cm, add PDA medium (about the 40 ℃) 14mL for preparing again, make the abundant mixing of extract and medium, poisonous culture medium flat plate is made in cooling, after insert respectively for examination bacterium cake (d=4mm), each bacterial classification is established 3 repetitions.The extraction solvent culture medium flat plate for preparing respective amount with same method in contrast.Each bacterium colony is measured by the right-angled intersection method, represents the size of bacterium colony with its mean value.The computing formula of bacteriostasis rate is as follows: pure amount of growth=bacterium colony average diameter-bacterium cake diameter; Bacteriostasis rate=[(contrast pure amount of growth-handle pure amount of growth)/contrast pure amount of growth] * 100%.
Table 1 green peel of walnut different solvents extract is measured the bacteriostasis of various pathogenic bacteria
Tab.1?Antifungal?effect?mensuration?of?the?different?solvents?extracts?from?Walnut?Peelagainst?different?hytopathogenic?fungi
A. ring rot of apple bacterium Physalospora nasei; B. Valsa mali Valsa mali; C. Monilinia fructicola Monilinia fiucticola; D. alternaria leaf spot of apple bacterium Alternaria alternata; E. eggplant eggplant rot bacterium Fusarium oxysporum; F. fusarium graminearum Botrytis cinerea.
Experimental result: as can be seen from Table 1, to the inhibition effect of ring rot of apple bacterium best be water extract, inhibiting rate reaches 96.88%; The green peel of walnut water extract is best to the inhibition effect of Valsa mali, and inhibiting rate is 100%; The inhibition DeGrain of chloroform extract, inhibiting rate are 36.78%; Green peel of walnut ligroin extraction and ethyl acetate extract are to the inhibition weak effect of Monilinia fructicola, and other 4 kinds of extracts are apparent in view to the inhibition effect to Monilinia fructicola, and the inhibition effect of water extract is strong, and inhibiting rate reaches 100%; The highest to alternaria leaf spot of apple bacterium water extract, bacteriostasis rate reaches 100%, and the inhibition effect of ethyl acetate and methanolic extract is comparatively obvious, the inhibition weak effect of benzinum and chloroform extract; Inhibition effect to eggplant eggplant rot bacterium water extract is best, inhibiting rate is 79.14%, and the ethyl acetate extract inhibiting rate is 30.54%, and other 3 kinds of extracts all have the stimulating growth effect to eggplant, the spread effect of ligroin extraction is particularly outstanding, and inhibiting rate is 41.63%; To the inhibition effect of fusarium graminearum best be water extract, inhibiting rate is 81.31%, other 4 kinds of extracts also have the inhibition effect.
Following embodiment all can realize the effect of above-mentioned experimental example
Embodiment
Embodiment 1: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 64kg, add the benzinum of 4 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than under 40 ℃ in temperature and is concentrated into 32L, ligroin extraction.
Embodiment 2: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 68kg, add the chloroform of 5 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than under 40 ℃ in temperature and is concentrated into 30L, chloroform extract.
Experimental example 3: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 70kg, add the methyl alcohol of 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, methanolic extract.
Experimental example 4: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 60kg, add the water of 8 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, water extract.
Experimental example 5: walnut peel extract and preparation method thereof
Take by weighing green peel of walnut powder 60kg, add the ethyl acetate of 8 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 28 parts by volume under 40 ℃, ethyl acetate extract.
Claims (6)
1. the application of green peel of walnut water extract in preparing the bactericidal agent that suppresses ring rot of apple bacterium, inhibition Monilinia fructicola, inhibition alternaria leaf spot of apple bacterium, inhibition eggplant eggplant rot bacterium, inhibition fusarium graminearum or suppress Valsa mali, described walnut peel extract is made by following method:
Take by weighing green peel of walnut powder 60-70 weight portion, add the water of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into the 28-35 parts by volume under 40 ℃, water extract.
2. the application of walnut peel extract as claimed in claim 1, described walnut peel extract is made by following method:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the water of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, water extract.
3. the application of green peel of walnut ethyl acetate extract in the bactericidal agent of preparation inhibition Monilinia fructicola, described walnut peel extract is made by following method:
Take by weighing green peel of walnut powder 60-70 weight portion, add the ethyl acetate of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into the 28-35 parts by volume under 40 ℃, ethyl acetate extract.
4. the application of walnut peel extract as claimed in claim 3, described walnut peel extract is made by following method:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the ethyl acetate of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, ethyl acetate extract.
5. the application of green peel of walnut methanolic extract in the bactericidal agent of preparation inhibition Monilinia fructicola, described walnut peel extract is made by following method:
Take by weighing green peel of walnut powder 60-70 weight portion, add the methyl alcohol of 3-8 times of weight of dry powder, lixiviate is 2-4 time on shaking table under the 3-6 ℃ of cryogenic conditions, leaves standstill 1-3 hour at every turn, shakes 1-3 hour; Extract is filtered, is lower than in temperature and is concentrated into the 28-35 parts by volume under 40 ℃, methanolic extract.
6. the application of walnut peel extract as claimed in claim 5 is characterized in that described walnut peel extract made by following method:
Take by weighing green peel of walnut powder 64 or 68 weight portions, add the methyl alcohol of 4,5 or 6 times of weight of dry powder, lixiviate is 3 times on shaking table under 4 or 5 ℃ of cryogenic conditions, leaves standstill 2 hours at every turn, shakes 2 hours; Extract is filtered, is lower than in temperature and is concentrated into 32 parts by volume under 40 ℃, methanolic extract.
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| CN200810056515A CN101416644B (en) | 2008-01-21 | 2008-01-21 | Walnut peel extract and new use thereof |
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Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101618066B (en) * | 2009-08-04 | 2011-09-28 | 天津科技大学 | Preparation method of walnut green husk total tannin and application of same |
| CN103766409A (en) * | 2012-10-18 | 2014-05-07 | 王涵 | Walnut green seedcase extract product and bacteriostatic use thereof |
| CN103222486B (en) * | 2013-05-23 | 2014-11-19 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
| CN103461470A (en) * | 2013-09-03 | 2013-12-25 | 新疆农业科学院农产品贮藏加工研究所 | Walnut green seedcase extract compound preservative as well as preparation method and application thereof |
| CN104872195A (en) * | 2015-04-16 | 2015-09-02 | 甘肃省分析测试中心 | Green walnut epicarp extract, extracting method and application of extract, and seed coating agent prepared from extract |
| CN104872207A (en) * | 2015-05-11 | 2015-09-02 | 刘全 | Insecticide bactericide for plants, and preparation method of insecticide bactericide |
| CN104872191A (en) * | 2015-06-16 | 2015-09-02 | 王鲜花 | Drug for curing gummy stem blight and manufacturing method thereof |
| CN106490001B (en) * | 2015-09-06 | 2019-01-22 | 新疆农业科学院海南三亚农作物育种试验中心 | It is a kind of using walnut peel extract as the thermal fog of effective component and its application |
| CN105851064A (en) * | 2016-04-06 | 2016-08-17 | 陕西省烟草公司商洛市公司 | Preparation method of walnut green husk extracts for preventing and treating tobacco viral disease |
| CN106614763A (en) * | 2016-11-15 | 2017-05-10 | 济南华鲁食品有限公司 | Method for preparing green insecticide from walnut green seedcase |
| CN107821447A (en) * | 2017-11-30 | 2018-03-23 | 孙铭岐 | A kind of walnut peel extract bactericide and its application process in preventing and treating Fusarium solani |
| CN108409410A (en) * | 2018-03-23 | 2018-08-17 | 山西省农业科学院农业环境与资源研究所 | A kind of antibacterial liquid and preparation method thereof for fruit tree rejuvenation greening |
| CN109111300A (en) * | 2018-11-06 | 2019-01-01 | 山东省葡萄研究院 | A kind of grape special nutritional bacteriostatic agent and the preparation method and application thereof |
| CN109452327A (en) * | 2018-12-18 | 2019-03-12 | 贵州省核桃研究所 | A kind of soil sterilants and its control method for preventing and treating early blight of tomato |
| CN110721570A (en) * | 2019-10-28 | 2020-01-24 | 北京城市排水集团有限责任公司 | Chinese herbal medicine deodorization composition and preparation method thereof |
| CN115226734B (en) * | 2022-08-25 | 2023-07-25 | 渭南职业技术学院 | Walnut green seedcase and jiuxin vegetable composite agricultural enzyme composition and preparation method and application thereof |
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| CN1781376A (en) * | 2004-12-03 | 2006-06-07 | 武汉绿世纪生物工程有限责任公司 | Use of walnut plant extract as pesticide in preventing and controlling diamondback moth |
| CN101099495A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Hickory nut husk disinfectant and its producing method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1781376A (en) * | 2004-12-03 | 2006-06-07 | 武汉绿世纪生物工程有限责任公司 | Use of walnut plant extract as pesticide in preventing and controlling diamondback moth |
| CN101099495A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Hickory nut husk disinfectant and its producing method |
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