CN104286563A - Green ecological medicinal deer feed and preparation method thereof - Google Patents

Abstract

The invention relates to the field of a medicinal feed, and provides a green ecological medicinal deer feed and a preparation method thereof. The feed comprises the following raw materials in parts by weight: 3-8 parts of pricklyash peel leaves, 8-13 parts of pomegranate leaves, 5-15 parts of willow leaves, 5-15 parts of walnut leaves and 1-5 parts of bran; the preparation method comprises the following steps: (a) cleaning the leaves in a NaCl aqueous solution according to weight ratio, and drying the leaves; (b)uniformly mixing the dry leaves with the bran, diluting a prepared one equal part of vinegar mother in 100kg of cold boiled water, and uniformly spraying the solution; (c) using a cylinder, a barrel and the like as storage fermentors, covering and sealing the storage fermentors with plastic films until the compost is fermented thoroughly; and (d) preparing the vinegar mother, namely culturing 3-4ml of edible alcohol, 0.4g of glucose, 1g of yeast cream, 1.5g of calcium carbonate, 1.5g of agar and 100g of water in the culture medium formula of one equal part of vinegar mother for 2 days at 30 DEG C, and storing the vinegar mother in a refrigerator (4 DEG C). The green ecological medicinal deer feed is used for changing waste materials into things of value, achieving homology of medicine and food and comprehensively providing the nutrient elements necessary for the growth of deer.

Description

Medicinal deer feed of a kind of green ecological and preparation method thereof

One, technical field

The present invention relates to medicinal deer field of fodder, be specifically related to medicinal deer feed in green medical feed field, particularly green ecological and preparation method thereof.

Two, background technology

According to China's development low-carbon (LC), circulation, organic agriculture, the agricultural development planning ensured food safety, in line with improving livestock products quality, reducing the object of production cost, research green ecological medical feed becomes a kind of inexorable trend.The medicinal deer feed of green ecological is the one of green safety feed.

Green refers to the green plants body (leaf) as feed.Leaf is a kind of nutrient biological feedstuff source completely.Green feed is not only the effective ways adding feed source, and also have great role to raising livestock products quality, specifically, green ecological medicinal deer feed has following advantage:

(1) according to estimates, China produces the leaf of several hundred million tons every year, and its quantity is also in continuous increase, has increased the weight of the pressure of urban garbage treatment system.Past, these leaves ran its course, and had wasted the ecological ideas feed of a large amount of cheapness.Containing abundant nutritional labeling in leaf, comprise crude protein, crude fibre, the inorganic constituents such as the organic principles such as crude fat and calcium, phosphorus, potassium.If collected by these leaves, Processing farage becomes agreeable to the taste feed, can turn waste into wealth exactly, and environmental protect, is increased economic efficiency simultaneously; And improve livestock products quality

(2) there are abundant forest resourceies in China, and leaf quantity is large, and except minority, great majority all can be feeding.Leaf is nutritious, after Processing farage, can make the feed of livestock and poultry.Leaf can be divided into the large class of needle, broad-leaved two by feature.One, the feeding value of the feeding value leaf of leaf is decided by factors: the nutritional labeling of (1) seeds leaf is different because of seeds, some seeds, as crude protein content in the leaves such as Leguminosae tree, elm, paper mulberry is higher, by dry gauge, all more than 20%, but also 18 seed amino acids containing constitutive protein matter, as pine needle; And organic matter content, the digestibility of the leaves such as paper mulberry, Chinese scholartree, willow, pear tree, peach, jujube tree, can be worth higher, more than 8.36 million Jiao/kilogram dries are reached to the digestible energy value of pig, 6.27 million Jiao/gram of dry matter is reached to the metabolizable energy value of chicken; In leaf, vitamin content is very high, according to analysis, in the leafiness such as willow, firelight or sunlight, excellent, alder, carotene carotene content is 110-132 mg/kg, the blue or green cured leaf carotene carotene content of false indigo can reach 270 mg/kg, carotene carotene content in needle, up to 197-344 mg/kg, contains the mineral matter element needed for livestock and poultry containing pine needle meals such as a large amount of vitamin C, vitamin E, vitamin D, vitamin K and Cobastab I in addition; Some leaves contain hormone, can stimulate the growth of livestock and poultry, or containing suppressing the bacteriocidin etc. of pathogen.(2) the fresh and tender leaf grown growth period is of high nutritive value; Blue or green fallen leaves take second place, and can be used for feeding and instead work as domestic animal, be also suitable for monogastric of feeding.

(3) leaf nutrient enriches, according to Beijing feed public apple, mountain peach, apricot for gathering at the beginning of 10 months, adding 13 kinds of leaves such as poplar, linden, willow, walnut, elm and analyze, nutritional labeling average out to s moisture 5.12%, crude protein 12.27%, ash content 10.96%.General leaf crude fiber content is less, protein, vitamin and content of mineral substances are more, as false indigo, locust tree, the protein content of the leaf such as silk tree, shrub lespedeza is all more than 20%, and in the blue or green cured leafs such as peach, elm, mulberry, poplar, the content of protein is also all more than 18%; The carotene content of the blue or green leaf of the per kilograms such as willow, birch, hazel, alder is 110-132 milligram, more than the content of yellow maize about 100 times.The carotene content of false indigo leaf, per kilogram can reach 270 milligrams; Not only there is abundant carotene in the needle of coniferals tree, also have injection Vitamin B_6, D, B., K etc., and containing various trace elements such as iron, cobalt, manganese; In leaf, calcareous content is generally at 1-2%, and as jujube, apple, pears, peach etc., more than 1.5%, are 25-50 times of the calcium content such as corn, Chinese sorghum; In the leaves such as elm, apricot, peach, two calyx Abelia bifloras, starch is also a lot, NFE be equivalent to pearling cone meal, corn flour content 2/3rds.The leaf of mountain area, upper level is abundanter, and exploitation leaf resource is to the utilization rate improving the forest reserves, and prosperity Mountainous Areas economic dispatch has positive effect.

(4) deer is also the economic class herding poultry kind of consumption grain type, also a certain amount of grain consumption is had, be main mainly with corn-soybean meal in the formula of conventional mixed feed, because provision price constantly rises, and this formula nutritional kind is more single, therefore, feed variation must be developed, and the kind containing abundant nutrition, reduce feed cost, reduce the import of corn, dregs of beans, three tons of leaves can replace a ton-grain food according to the literature.The present invention mainly evades people and animals with regard to leaf as the development and utilization of feed and strives grain; Guarantee grain security! ! Most of leaf can be used as feed.China is the vast large agricultural country in soil, and forest land about 2,670,000 km2, wherein area of woods 1,250,000 km2, add that other whole nations such as shrub, fruit tree leaves can produce leaf dried powder 1 000 ten thousand more than t per year.The leaf that general wealthy leaf nutritive value crude protein contains 4% ~ 6% has white poplar leaf, mountain Stand-for-sacrifice leaf, wych-elm leaf, willow leaf, foreign outer coffin leaf, autumn white pear leaf, soft pears leaf, the wooden leaf of crow bubble, bubble wood leaf, lamp stand leaf, rascal leaf, ebon leaf, thousand zhang of leaves, camellia leaf, peach leaf, common camptotheca leaf, Chinese alangium leaf, Chinese holly leaf, Leaves of Corylus heterophylla Fish. ex Bess, Chinese hackberry leaf, the cold leaf of plum, smallfruit fig leaf, Chinese tallow tree leaf etc.Crude protein has Siberian elm leaf, pear with a long stem leaf, apple leaf, bitter Chinese toon leaf, winter folium mori, blue or green thick stick leaf, stone Shu Ye, mongolian oak leaf, Chinese pear-leaved crabapple page, leaf of Diospyros kaki L.f, bodhi leaf etc. containing the leaf of 7% ~ 10%.Crude protein has wild Albizzia kalkora leaf, Huang Huaiye, Folium Pruni, lime tree leaf, terrible leaf, Malus spectabilis leaf, Ci Ye, Folium Eucommiae, wild paulownia leaf, Chinese gall, cercis leaf etc. containing the leaf of 11% ~ 15%.

At present to the existing a small amount of research of green feed, as number of patent application: 200810 ... disclose a kind of biological multi-effect feed, by

As number of patent application: 200910 ... disclose a kind of multifunctional feed, be made up of the raw material of following parts by weight: ... multifunctional feed production technology of the present invention is simple, and comprehensive one-tenth wood is low, applied widely.

Current existing green feed is not developed for certain poultry specially, and its specific aim is not strong.

Three, summary of the invention

For above the deficiencies in the prior art, the invention provides medicinal deer feed of a kind of green ecological and preparation method thereof.The medicinal deer feed of this green ecological achieves " turning waste into wealth ", " integration of drinking and medicinal herbs ", has the function of low-carbon (LC) safety and raising animal husbandry product quality.

Technical scheme of the present invention is:

The medicinal deer feed of a kind of green ecological,

(1) weight portion of raw material consists of:

Chinese prickly ash leaf 3-8 part,

Pomegranate leaf 8-13 part,

Folium Pterocaryae 5-15 part,

Walnut leaf 5-15 part,

Wheat bran 1-5 part

(2) described green ecological deer pig feed produced according to the following steps by the raw material getting described weight portion:

A) get bunge pricklyash leaf, Folium Pterocaryae, pomegranate leaf, walnut leaf, clean in the NaCl aqueous solution according to above-mentioned weight, dry;

B) by the leaf that dries and, wheat bran to stir in whipping process will prepare 1 decile mother of vinegar cold water 100kg dilute after evenly spray (the mother of vinegar liquid after every 100kg dilution can process 1000 kilograms of leaves and bran mixture).

C) storage is held fermentor available cylinder, bucket, cement pit etc. and with plastic sheeting, fermentor is held in storage and covers airtight good (lucifuge), until compost fermentation become thoroughly decomposed and get final product (can smell wine, vinegar fragrance time).

D) preparation of mother of vinegar: 1 decile mother of vinegar culture medium prescription: edible alcohol 3-4ml, glucose 0.4g, yeast extract 1g, calcium carbonate 1.5g, agar 1.5g, water 100g.Cultivate: cultivate after 2 days at 30 DEG C, Refrigerator store (4 DEG C).

One of meet the following conditions, be the medicinal deer feed fermentation of described green ecological ripe:

1) fermentate is in yellow;

2) brown liquid is had to overflow;

3) volume ratio of fermentate decreases 2/3 to 1/2 before comparatively fermenting;

4) fragrance of wine, vinegar can be smelt.

5) possess sour, sweet, fragrant, soft, ripe.

The described green ecological medicinal deer feed fermentation time is 5 days to 15 days.

The present invention will be further explained below:

Chinese prickly ash of the present invention is rutaceae green pepper and Chinese prickly ash; Described willow is the full section of Dilleniidae Salix; Described pomegranate tree is the full section of Punicaceae; Described walnut is the full section of juglans.

The present invention is by lot of experiments and select excellent, obtains and is suitable for the medicinal deer feed of green ecological that safety and Health raises deer, and its know-why being particularly conducive to deer growth has:

A. control effect and mechanism (pharmacological action):

" 1 " bunge pricklyash leaf Advances on Effective Compositions:

A) chemical composition of Chinese materia medica of bunge pricklyash leaf: Chinese prickly ash contains volatile oil, alkaloid, acid amides, cumarin, flavone compound, aliphatic acid, sterol, lignanoid etc., as Quercetin-3-rhamnoside, hyperin, miscandenin, palmitic acid, leukotrienes, oleic acid, cupreol, nonacosane etc.

Fu Chenmei etc. are to Chinese prickly ash research, and it mainly contains chemical composition is volatile oil, alkaloid, acid amides, cumarin, aliphatic acid etc., isolates olefines, alcohols, ketone, epoxide class and ester class from volatile oil.Chinese prickly ash contains the amide substance that a large amount of numb taste is insoluble in water: hydroxy-beta-sanshool, hydroxyl-γ-sanshool, hydroxyl-alpha-sanshool, γ-sanshool, 2 '-hydroxy-n-isobutyl group-2.4.8.10.12-tetradecane nonenamide, 2 '-hydroxy-n-isobutyl group-2.4.8.11-tetradecane pentaene acid amides, 2 '-hydroxy-n-isobutyl group-2.6.8.10-22 carbon tetraene acid amides, 2 '-hydroxy-n-isobutyl group-2.6.8.10-dodecane tetraene acid amides, N-para hydroxybenzene ylmethyl-2.7-dimethyl-2.6 octadiene acid amides, alkaloids has quinoline, isoquinilone derivatives, benzophenanthrene piperidine derivatives, skimmianine, 6-methoxyl group-5.6 dihydrochelerythrine, vanilla lemon alkali, conjunction Pa Luoping alkali, specially-N-methyl-Chelerythrine, p-cymene, 2,5-1.1-dimethyl ethyl thiophene, piperitone, aliphatic acid, lignanoid, Quercetin-3-rhamnoside, Hyperoside, miscandenin, palmitic acid, leukotrienes, oleic acid, cupreol etc.

Zheng Jin etc. study Chinese prickly ash and contain natural nitrogenous compounds alkaloid, as quinoline, isoquinilone derivatives, benzophenanthrene piperidine derivatives, Carbostyril derivative, exist mainly with free and quaternary amine form, find that there is skimmianine, green pepper alkali, vanilla lemon, handkerchief Lip river equality, green pepper alkali is 4-quinolinones alkaloid; Chinese prickly ash also containing the chain unrighted acid amides compound of numb taste main source, Here it is sanshool; The coumarin kind compounds such as the lactone of Chinese prickly ash also containing adjacent hydroxyl cinnamic acid; Chinese prickly ash is also containing the dual-ring phase structure that bimolecular phenyl propanoid derivative is polymerized; Triterpene, sterol, alkane, flavonoid glycoside etc.

B) bunge pricklyash leaf infusion and extract thereof all have inhibitory action to staphylococcus aureus, MRSE, hay bacillus, sarcine, EHEC, typhoid bacillus, alpha streptococcus, streptococcus pneumonia, Bacillus influenzae, streptococcus meningitidis etc.; Bunge pricklyash leaf (pericarp) water extract has obvious inhibitory action to Gram-positives such as Gram-negative enteric pathogenic bacteria and staphylococcus aureus such as shigella dysenteriaes addicted to oxygen bacterium; Xanthoxylin significantly can suppress the generation of platelet adhesion reaction and glycerine (MDA); The alcohol extract of bunge pricklyash leaf (pericarp) has active anticancer; Chinese prickly ash pericarp volatile oil has narcotic analgesic effect, inhibited to corynebacterium diphtheriae, bacillus anthracis, Diplococcus pneumopniae, staphylococcus aureus, typhoid bacillus, Pseudomonas aeruginosa and some dermatophyte; Also there is the effect killing ascaris suum.Yu Bailiang (1998) have studied xanthoxylum oleoresin to several mould, bacterium, saccharomycetic minimum inhibitory concentration, have detected the active pH scope of xanthoxylum oleoresin, and has carried out antibacterial efficacy with spice powder and Sodium Benzoate and compare; Cheng Bo etc. determine the bactericidal action of pericarpium zanthoxyli volatile oil to some pathomycete, result display pericarpium zanthoxyli volatile oil all has certain antibacterial and bactericidal action to 11 kinds of dermatophytes and 4 kinds of deep fungals, wherein with wool sample sporidiole bacteria and Trichophyton rubrum the most responsive, minimum inhibitory concentration (MIC) is lower than 0.1 μ L/mL, and minimum bactericidal concentration (MFC) is 0.1 μ L/mL.Experiment also confirms that moon Gui Dan Zhuo ketone and dimethyl sulfoxide (DMSO) can promote that volatile oil enters in fungal cell, accelerate cell death;

C) in addition Chinese prickly ash also has other using value: (1) insect-pest: pepper volatile oil to sitophilus zea-mais, intend the storage pests such as ostomatid and grain storage aspergillus, mould has prevention effect; (2) anti-oxidant: to add in the animal and plant fats such as lard and can grease-proof ruin; (3) bacteriostasis: as previously mentioned, can have stronger inhibitory action to mould, bacterium, saccharomycete etc.; The preventive effect to sitophilus zea-mais, red flour beetle grain storage aspergillus and mould is studied to the crude extract of Chinese prickly ash and volatile oil for (1970) and the Jiang little Long etc. (1992) such as Zhu Guoyi (1981), I Sinko; The people such as Lu Chunming (1995,1996) composition of Chinese prickly ash ethereal extract is studied, and think that β-phellandrene in pepper volatile oil and linalool are the active components of killing confused flour beetle adult, confused flour beetle adult can be made to produce nerve poison, virulence than common pesticides malathion and triumphant security much better than, and research finds that in pepper volatile oil, β-phellandrene content is the highest, reach 12.97%, linalool content is 2.09%;

D) bunge pricklyash leaf water extract also has the function suppressing weed growth.In the plant of section more than 30, find that hundreds of has the natural plants toxin of activity of weeding in the world at present.Botanical herbicides active ingredient is mainly quinone phenols, alkaloids, cinnamic acid, Coumarins, thiophene-based, flavonoid class, terpenes, amino acids etc. in structure chemically.

E) bibliography:

[1] Zhang Quan, yellow wise, Min Zhongcheng etc. the progress of the uracil compound of [J] tool activity of weeding. agricultural chemicals, 2006,45 (2), 73-77.

[2] Mao Jingying, Yan Zhenling. " plant growth regulator principle of adjustment and control and practical technique ". Chinese agriculture publishing house, in January, 2005 first edition.

[3] Li Xiaofei, Feng Huacheng. administer the native compound [J] of weeds. world pesticide, 2000.22 (3): 20-24.

[4] by the development and utilization [J] of the natural weeds compound of state of shaking. weeds journal, 1993.7 (2): 40-48.

[5]Boyeue?C?D，etal.Progress?in?the?productionnnulation，andapplication?of?myco?herbicides[A]lIn：Tebeest?D01Microbial?control?ofweed[M]1New?York：Chapman?andHall，1991，209-222.

[6] Li Wei, Xiao Xianglin, Zhang Danyan, conventional Chinese medicine thin-layer chromatography qualification [M]. Beijing. chemical industry publishing house, 2005:196

[7] Fu Chenmei, Kan Jianquan, Chen Zongdao, China of State of Zhao, the composition Study of Chinese prickly ash and application [J] thereof, Food Additives Used in China, 2003 (4): 83-84

[8] Wang Shuangui, Zhao Jingxian, Bi Jun, Zhi Enbo, the present Research of domestic and international Chinese prickly ash and development trend [J] thereof, Inner Mongol forestry science and technology, 2003 (2): 32-34

[9] Chen Zhende, Xu Chongyuan, Xie Li. the research [J] of supercritical carbon dioxide extraction pepper volatile oil chemical composition. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2001,26 (10): 687-688

[10] Liu Suolan, the analysis [J] of two kinds of medicinal pepper volatile oils, CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1991,16 (6): 359-360

[11] Zheng Jin, Zhang Lijuan, Chinese prickly ash Medicinal Study Development [J], Liaoning University of TCM's journal, 2008 (11): 69-71

[12] Sun little Wen. Duan Zhixing. Zanthoxylum medicinal plants study progress ∞. Acta Pharmaceutica Sinica .1996,31 (3): 231.

[13] Zhang Mingfa. Fan Rongpei, Guo Huiling. etc. drugs for dispelling internal cold analgesic activity research (J). the Shaanxi traditional Chinese medical science.1989；(10)：231.

[14] Zhang Mingfa. Shen Yaqin, Zhu Ziping, etc. Chinese prickly ash antalgic and warming middle energizer to stop diarrhea pharmacological research (J). Chinese medicine.1994。17(2)：37.

[15] Chang Zhiqing. Wang Shuling, Hao Changyuan, etc. bacterium taro alkali analgesia, separate cancer and sedation (J). Acta Pharmacologica Sinica.1982，(3)：163.

[16] Tong Ruxin. etc. Liaoning pericarpium zanthoxyli schinifolii and pericarpium zanthoxyli acute toxicity pharmacological action comparative studies [J] Liaoning Journal of Traditional Chinese Medicine 1995.22(8)：371.373.

[17] Tong Ruxin, king's sauce people. Zhang Huiying. etc. the pharmacological experiment study (J) of pericarpium zanthoxyli schinifolii active component bergapten. Chinese traditional Chinese medicine information magazine .1999; 6 (10): 30.

[18] Sun Hongfan, etc. the research U of Chinese prickly ash blocking nerves impulse action]. Guizhou medicine, 1990;

" 2 " pomegranate leaf Advances on Effective Compositions:

(1) the effective medicinal ingredient of pomegranate leaf:

Bibliographical information has been separated and has obtained more than 20 and plant compound from pomegranate leaf, comprises ursolic acid, ginkgolic acid, betulic acid, sweet mellow wine, flavonoid glycoside compound, sprout money tannin and Ellagitannins etc. (4).Researcher be separated first obtain ellagic acid (sigh-1>, brevifolin (sigh-2>, gallic acid (sigh-3> and reseda 4 '-.--glucoside (sigh-5>, tannins mainly contains polyenzyme, tannin, pelletierine, isoquercitrin, resin, sweet mellow wine, gallic acid etc., and containing several amino acids and various trace elements.

(2) antiviral, the bacteriostasis of pomegranate leaf:

According to related documents report, a. antivirus action: the main active tannin in pomegranate leaf, be a kind of water-soluble polyhydric phenols with precipitating proteins characteristic, antiviral functions utilizes this characteristic exactly.Virus inoculation after pomegranate leaf (skin) water extract of variable concentrations and cytosis 2h, result shows, along with the reinforcement of concentration, antivirus action is strengthened.The pomegranate leaf water decoction gelatine microsphere having anti-HSV-2 (II herpes simplex virus type) to act on is eliminated the tannin composition of pomegranate leaf, in pomegranate liquid, antivirus action obviously weakens.

B. antibacterial action: the tannin of pomegranate leaf can solidify the plasm of microbial body and multiple enzyme because of it, has inhibitory action to various bacterium and fungi.Zhou Benhong etc. carry out clinical research to each component of tannin in pomegranate leaf to gonococcus sensitiveness, prove that granatum has obvious inhibitory action to gonococcus.Pomegranate leaf among the people is often used by people to treatment diarrhoea, dysentery, aphtha, abscess, pharyngalgia, urinary tract inflammation, scabies, skin injury and hemostasis etc.

C. bibliography:

[1] State Administration of Traditional Chinese Medicine. Chinese herbal editorial board. book on Chinese herbal medicine (the 5th volume) extra large science tech publishing house of China

[2] Li Dingge, Zhang Kui, Lin Qingyi, etc. the experimental study ^ Pharmacology and Clinics of Chinese Materia Medica that pomegranate leaf affects digestive function, 1998,14 (0:35 6

[3] Li Dingge, Zhang Zengmin, Chen Xiaojian, etc. the pomegranate leaf water logging agent research (Pharmacology and Clinics of Chinese Materia Medica) to rat tolerance secretion and tentative gastric ulcer effect

[4] Hu Wei, Dai Wei, Yang Yumei, etc. granatum is studied the antibacterial experiment in vitro of helicobacter pylori.The Academic Journal of Kunming Medical College, 25 8

[5] Li Dingge, Su Chuanqin, Sun Li, etc. the research of pomegranate leaf adjusting blood lipid and scavenging activated oxygen effect. Shandong Traditional Chinese Medicine University's journal: 380 9

[6] discriminate the Meng, Sun Lihong, Chen Yunyun, etc. pomegranate leaf tannin is on the lipometabolic impact of high fat of blood blood sugar animal pattern. Chinese experimental pharmacology of traditional Chinese medical formulae magazine

[7] Li Yunfeng, Guo Changjiang, Yang Jijun, etc. Punica granatum L. extract is to the comparison of oxidative stress Efficacy of Protecting Vascular Endothelial. Chinese Clinical rehabilitation, 2006

[8] Zhang Jie, Zhan Ping Yan, Yao Xuejun, etc. the effect of Chinese medicine Tannin in Punica granatum L composition anti-genitals herpesviral. CHINA JOURNAL OF CHINESE MATERIA MEDICA,

[9] Zhou Benhong, Liu Chun, Chen Lei, etc. granatum is to gonococcus Study of Sensitivity.Chinese Pharmaceutical Journal, 65

[10] pomegranate leaf and granatum pharmacology activity research progress Du Xin, Ye Shengying (No.254 Hospital, PLA, Tianjin 300142>

(3) chemical composition of vinegar

Vinegar is the aqueous solution of 3% ~ 5% (mass fraction) of acetic acid.In addition to acetic acid, general some micro substances containing other.Concrete material has the potassium of acetic acid, calcium, sodium, ferrous salt, amino acid, lactic acid (2 hydroxy propanoic acid, lactic acid CH3CHOHCOOH), pyruvic acid (acetyl formic acid CH3COCOOH), formic acid (formic acid HCOOH), sorbic acid (2, 4-hexadienoic acid) malic acid (hydroxysuccinic acid), citric acid (2-hydroxy propane tricarboxylic acid), butanedioic acid acid (succinic acid HOOCCH2CH2COOH), oxalic acid (ethanedioic acid HOOCCOOH), oxaloacetic acid, glucose, fructose, maltose, ethanol, ethyl acetate, ethyl lactate, higher alcohols, 3-Hydroxybutanone, dihydroxyacetone (DHA), tyrosol, acetaldehyde, formaldehyde, acetal, vitamin B1, B2, C etc.

(4) sterilization mechanism of vinegar is: low pH value (less than 2.7) and the high ORP (more than+1100mv) of acidic oxidized electric potential water, beyond the scope of various pathogenic microorganism, and the film potential of its cell is changed, to cause the destruction of permeability of cell membrane enhancing and cellular metabolism enzyme, microorganism is dead rapidly.Meanwhile, the effective chlorine of the 25 ~ 60mg/L contained in acidic oxidized electric potential water can more effectively pathogenic microbe killing.

(5) vinegar is broad-spectrum sterilization, bacteriostatic agent: Escherichia coli (Escherichia coli), aerobacteria (Aerobateraerogenes), staphylococcus aureus (Staphylococcus aureus), Bacterium prodigiosum (Bacillus prodigiosus).

Trichoderma viride (Trichoderma viride), koning trichoderma (Trichoderrnakoningii), mould (Penicillium glaucum), aspergillus niger (Aspergillus niger).

Cucumber anthracnose (Colletotrichumlagenarium), Plasmopara viticola (Plasmopara viticola), verticillium dahliae (Verticillium dahliae), P. capsici (Phytophthora capsici), fusarium graminearum (Fusariumgraminearum) and Valsa mali (Valsa mali)

(6) traditional Chinese medical science function of vinegar: the loose stasis of blood, hemostasis, removing toxic substances, desinsection.Come from " Mingyi Bielu ", Tang Materia Medica, supplement to the Herbal, Compendium of Material Medica:

" 3 " Folium Pterocaryae Advances on Effective Compositions:

A. the organic principle in Folium Pterocaryae: aspirin is a kind of medicine with effects such as antipyretic and analgesias that the mankind commonly use, and its formal name used at school is acetylsalicylic acid.The invention of aspirin originates from the willow be seen everywhere.Containing abundant natural medicinal component, as polysaccharide, flavones, phenol, vegetable protein (little peptide), saponin(e, coumarin, alkaloid, steroidal, terpene, glycoside, the trace element of the chemical compositions such as organic acid, carrotene, vitamin E, caffeine and some needed by human, the people such as Yi Xing are separated first and obtain 3 flavonoid monomer compound from Qingqian Willow leaf: Kaempferol (Kaempferol, I), Quercetin (Quercetin, and isoquercitrin (Isoquercitrin, III) II) ^[12], and by reversed-phased high performace liquid chromatographic, measure the content of wherein flavones, recording result is: in blue or green money willow methanolic extract, flavones content is Isoquercitrin 0.543%, Quercetin 0.0615%, Kaempferol 0.0387%; Isoquercitrin 0.464% in water extract, Quercetin 0.0603%, Kaempferol 0.0337%.This research has important theoretical significance and application value to the research of blue or green money willow flavones ingredient ^{[1] [6] [7]}.

The ethanol extract of the people such as Zhang Xiaoqi to Qingqian Willow leaf is studied, obtain 6 compounds by multiple chromatographic isolation, be accredited as respectively through physics and chemistry and spectral method: vanillic acid, benzoic acid, Kaempferol 3-O-β-D-glucopyra aldehydic acid glycosides, Quercetin, cupreol and daucosterol.First three is planted compound and obtains for being separated first from this plant ^[21].The lipophilic moieties that Shu Rengeng etc. are rich in sweet taste from Qingqian Willow leaf extract isolates 20 monomeric compounds, identifies and infers the structure of 15 compounds.5 noval chemical compounds for nature discovery are: blue or green money willow glucoside I (cyclocarioside I), blue or green money willow glucoside II (cyclocarioside II), blue or green money willow glucoside III (cyclocariosideIII), cyclocarya paliurus acid I (cyclocaric acid I) and cyclocarya paliurus acid II (cyclocaric acidII).Other 10 is isolated known compound from this plant first, and they are respectively Daucosterol (daucosterol), β-L-arabopyranose (β-L-Arabopyranose), caffeine (caffeine), β-steroid paddy alcohol (β-sitosterol), palmitic acid (palmitic aacid), stearic acid (stearic acid), mountain Yu's acid (behenic acid), inositol (inositol), 2-α-ursolic acid (2-α-hydroxyl ursolic acid) and Arjunolic acid.Wherein blue or green money willow glucoside I (a kind of triterpenoid saponin) has sweet taste, and it is one of native compound with sweet taste found in the world, has very important researching value.The natural sweet taste chemical families that is found to be of blue or green money willow glucoside I adds new a member.Blue or green the mainly sweet of money willow hides the triterpenoid saponin that composition is non-calorific value type, especially as the same with the Stevioside in STEVIA REBAUDIANA in the glycyrrhizin in Radix Glycyrrhizae therefore, develop the blue or green money willow glucoside natural sweetener in blue or green money willow, better making full use of China's this peculiar resource of blue or green money willow, is the emphasis of research and development ^{[22] [23]}.

Phytosterol (mainly sitosterol, stigmasterol etc.) is the material that a class has strong physiologically active, can effectively reduce cholesterol levels in serum, play the effect of the angiocardiopathies such as prevention and therapy hypertension, coronary heart disease; There is anti-inflammatory and antibacterial action; There is the function blocking Induced by Carcinogen cancer cell and formed; Promote the synthesis of DNA and protein, suppress blood sugar to rise and promote to recover liver function; Promote sebum secretion and keep moisture, to maintain skin moisturizing, soft physiologically active and surface-active etc.Open Luo dawn etc. from blue or green money willow root, isolate 5 compounds first, contrast by Spectrum Analysis and with the literature value of known compound, determine that their structure is respectively: (+)-catechu rope, Spondias axillaris glycosides, Himalayan pine rope 7-O-gives a tongue-lashing glucopyranoside glycosides, cupreol and daucosterol.Wherein (+)-catechu rope has the drawing together property of the various liver diseases of antidiarrheal, removing toxic substances, hemostasis and treatment; Cupreol also has anticancer, effect such as reduction cholesterol, cough-relieving etc. ^{[24] [25]}.Triterpene substance also has very strong physiologically active, and as anti-inflammatory, analgesia, calm, antibacterial, antipyretic, expelling parasite, antiviral, inhibition cancer cell hyperplasia, antiallergy, anti-inflammatory, the metabolism of promotion liver, help and prevent the heart, blood, cerebral disease from occurring etc.Zhong Rui to build etc. from Qingqian Willow leaf, has separated 3 pentacyclic triterpene compositions first, through physicochemical constant and spectrum analysis be: 2a-hydroxyursolic acid, 3-oxo-2a, 23-dihydroxyolean-12-en-28-oic acid (cyclocarya paliurus acid B), arjunolic acid.Wherein cyclocarya paliurus acid B is a kind of noval chemical compound.The further research tool of the physiological active functions of blue or green money willow is had very important significance ^{[26] [27] [28]}.

B. control effect and mechanism: lactones has anticancer (nasopharyngeal carcinoma), anti-leukocythemia, cholinolytic effect, antihistamine, step-down is antibacterial, antiulcer, hemostasis, expelling parasite, anti-Leptospira, brings down a fever, hypoglycemic effect of Denging; Saponin has cardiac stimulant, vasodilator and antifatigue, and reducing heart rate and Bipolar blood pressure effect promote the biosynthesis of DNA and protein and lipid, slake thirst and help produce saliva, and apocenosis is subsided a swelling, relieving sore throat and acesodyne, the effects such as antipyretic-antalgic; Coumarin has anticancer, antibacterial, diuresis and protection vasopermeability, anti-inflammatory, anticoagulation, and coronary artery dilator, improves blood circulation, and quench the thirst Eradicates phlegm, relievings asthma, and absorbs ultraviolet and radioresistance, desinsection, and analgesia is antipyretic, during wind resistance, and step-down, the effects such as antihistamine.Triterpene substance also has very strong physiologically active, and as anti-inflammatory, analgesia, calm, antibacterial, antipyretic, expelling parasite, antiviral, inhibition cancer cell hyperplasia, antiallergy, anti-inflammatory, the metabolism of promotion liver, help and prevent the heart, blood, cerebral disease from occurring etc.

Above argument is enough to illustrate that blue or green money willow is a kind of functional medicinal plant.

C. bibliography:

[1] Xie Mingyong, Li Lei. blue or green money willowization studies and bioactivity research overview [J]. Chinese herbal medicine .2001,32 (4): 365 ~ 366

[2] easily wake up, Xie Mingyong, Wang Yuanxing, Deng Zeyuan, Huang Zhiyong. chromocor compound content [J] in the blue or green money willow of rp-hplc determination. University Of Nanchang's journal (natural sciences version) .2001,25 (2): 161 ~ 164

[3] Zhu Ben Feng, He Zhaodong, Wang Zhengfeng, Chen Ling. the general flavone content of the blue or green money willow of Different sources compares [J]. Strait Pharmaceutical Journal .2004,16 (3): 88 ~ 89

[4] Xie Mingyong, Yu Yingli, Wang Yuanxing, Yi Xing, Wang little Ru. blue or green money willow chromocor compound structure and content. University Of Nanchang's journal (natural sciences version) .2003,27 (1): 49 ~ 52

[5] Xie Mingyong, Wang Yuanxing, Yi Xing, Wang little Ru. chromocor compound structure and determination study in Qingqian Willow leaf. analytical chemistry research notes .2004,32 (8): 1053 ~ 1056

[6] Li Lei, Zhao Li, Xie Mingyong, Wang little Ru. blue or green money willow polysaccharide component biologically active and species analysis of elements thereof. Xiamen University's journal (natural science edition) .2002,42 (1): 73 ~ 77

[7] Shu Rengeng, Shu Jicheng, Liu Yufeng. the assay of polysaccharide in blue or green money willow tender leaf. Chinese traditional Chinese medicine information magazine .2005,12 (3): 45 ~ 46

[8] Li Lei, Xie Mingyong, Yi Xing, Wang little Ru. blue or green money willow polysaccharide component and hypoglycemic activity research thereof. Agricultural University Of Jiangxi's journal 2001,23 (4): 484 ~ 486

[9] easily wake up, stone is made contributions, and period-luminosity is male, Xie Mingyong. blue or green money willow chemical constitution study. and CHINA JOURNAL OF CHINESE MATERIA MEDICA .2002,27 (1): 43 ~ 45

[10] Peng Shanshan etc. the impact that the content of Mineral Elements in Oolong Tea and mode of making tea leach it

[11] Qin Meijie, He Weixiang, Guo Yanhua etc. effect of traditional tonic medicine and the research of Trace Element. trace elements and health studies .1998, and 15 (3): 18 ~ 49

[12] Li Lei, Xie Mingyong, Deng Zeyuan, Li Xiaoling, Wen Huiliang. the Primary Speciation of blue or green money willow inorganic elements. University Of Nanchang's journal (industry science version) .2000,22 (1): 74 ~ 77

[13] Zhang Xiaoqi, leaf literary talent, Yin Zhiqi, Zhang Zhenhua, Zhao Shouxun. the chemical constitution study of blue or green money willow. Chinese important magazine .2005,30 (10): 791 ~ 792

[14] Shu Rengeng. blue or green money willow plant chemical ingredient research notes. Jiangxi College of Traditional Chinese Medicine journal .1996,8 (2): 34

[15] Shu Rengeng, Xu Changrui, Li Lianniang. the research of blue or green money willow sweet ingredient. medical journal .1995,30 (10): 757 ~ 761

[16] Luo dawn is opened, Liao Xun, Ding Lisheng. the chemical composition of blue or green money willow. application and environmental organism journal .2001,7 (1): 90 ~ 91

[17] Zhong Ruijian, Shu Rengeng, Ni little Lan, Xu Changrui, Li Lianniang. the structural research of cyclocarya paliurus acid A. Acta Pharmaceutica Sinica .1991,31 (5): 398 ~ 400

[18] Zhong Ruijian, Gao Youheng, Xu Changrui, Li Lianniang. the research of pentacyclic triterpene composition in blue or green money willow. Chinese herbal medicine .1996,27 (7): 387 ~ 388

[19] Shu Rengeng, Liu Yufeng, Chen Jie, Shu Jicheng. the research of Triterpenoid in blue or green money willow plant. Chinese medicine .2005,28 (7): 558 ~ 559

[20] Fang Shengzuo, Yang Wanxia. the exploitation of blue or green money willow and Resource Cultivation. forestry science and technology develops .2003, and 17 (1): 49 ~ 51

[21] Shu Rengeng, Xu Changrui, Li Lianniang. blue or green money willow chemical constitution study (I). Chinese medicine .1996,18 (7): 351 ~ 352

" 4 " walnut leaf Advances on Effective Compositions:

(1) insecticidal action of walnut leaf active drug composition:

In alcohol extract from Juglans mandshurica Maxim leaves, main chemical compositions is juglone, and in alcohol extract from Juglans mandshurica Maxim leaves, the main active insecticidal components of chloroform extraction phase is the juglone that relative amount is the highest, i.e. Nucin; Other active insecticidal components may be symmetrical 2,2 ' methylene 6-0,1-dimethylethyl justice-methyl bisphenol and 2-methoxyl group 4-Vinyl phenol.

The test such as Sun Molong proves that juglone has extremely strong insecticidal activity to the larva of gypsymoth and lopper worm and adult.

(2) bacteriostasis of walnut leaf active drug composition:

Zhai Meizhi etc. have stronger inhibitory action to Walnut Leaves ethanol extract (compound such as juglanin, citrene, gallic acid) for staphylococcus aureus, bacillus subtilis, bread mold etc.

B. the effect mechanism of the medicinal pig feed of green ecological:

(1) green refers to the green plants body (leaf) as feed.Leaf is a kind of nutrient biological feedstuff source completely.Green feed is not only the effective ways adding feed source, also has great role to raising livestock products quality.Green pepper leaf in the present invention, Folium Pterocaryae, pomegranate leaf, walnut leaf belong to green plants body.

Compared with prior art, advantage of the present invention is:

1. the medicinal deer feed nutrient of green ecological of the present invention is complete, and especially vitamin cellulose content is high, and safety, ecological, environmental protective, saves food.

2. apply mother of vinegar fermentation process; The harmful element in leaf is made to be removed (combination reaction) such as tannin etc.

Four, detailed description of the invention

Embodiment 1:

The medicinal deer feed of a kind of green ecological described in the present embodiment, is made up of the raw material of following weight portion (kg): Chinese prickly ash leaf 3kg, pomegranate leaf 8kg, Folium Pterocaryae 29kg, walnut leaf 25kg, wheat bran 15kg;

According to above parts by weight of raw materials proportioning, preparation method comprises the following steps:

A) get bunge pricklyash leaf, Folium Pterocaryae, pomegranate leaf, walnut leaf, clean in the NaCl aqueous solution according to above-mentioned weight, dry;

B) by the leaf that dries and, wheat bran to stir in whipping process will prepare 1 decile mother of vinegar cold water 100kg dilute after evenly spray (the mother of vinegar liquid after every 100kg dilution can process 1000 kilograms of leaves and rice hulls mixture).

C) storage is held fermentor available cylinder, bucket, cement pit etc. and with plastic sheeting, fermentor is held in storage and covers airtight good (lucifuge), until compost fermentation become thoroughly decomposed and get final product (can smell wine, vinegar fragrance time).

D) preparation of mother of vinegar: 1 decile mother of vinegar culture medium prescription: edible alcohol 3-4ml, glucose 0.4g, yeast extract 1g, calcium carbonate 1.5g, agar 1.5g, water 100g.Cultivate: cultivate after 2 days at 30 DEG C, Refrigerator store (4 DEG C).

One of meet the following conditions, be the medicinal deer feed fermentation of described green ecological ripe:

1) object that ferments is yellow look;

2) brown or black liquor spilling is had;

3) volume ratio of compost comparatively decreases 2/3 to 1/2 before compost;

4) fragrance of wine, vinegar can be smelt.

5) possess sour, sweet, fragrant, soft, ripe

Described fermentation time is 5 days to 15 days.

Embodiment 2:

The medicinal deer feed of a kind of green ecological described in the present embodiment, is made up of the raw material of following weight portion (kg): Chinese prickly ash leaf 4kg, pomegranate leaf 6kg, Folium Pterocaryae 35kg, walnut leaf 35kg, wheat bran 20kg; The preparation method of a kind of green ecological medicinal deer feed described in the present embodiment is identical with embodiment 1 with step.

Embodiment 3:

The medicinal deer feed of a kind of green ecological described in the present embodiment, is made up of the raw material of following weight portion (kg): Chinese prickly ash leaf 3.5kg, pomegranate leaf 15kg, Folium Pterocaryae 26.5kg, walnut leaf 38kg, wheat bran 17kg; The preparation method of a kind of green ecological medicinal deer feed described in the present embodiment is identical with embodiment 1 with step.

Claims (5)

1. the medicinal deer feed of green ecological, is characterized in that:

(1) weight portion of raw material consists of:

Chinese prickly ash leaf 3-8 part,

Pomegranate leaf 8-13 part,

Folium Pterocaryae 5-15 part,

Walnut leaf 5-15 part,

Wheat bran 1-5 part

(2) the medicinal deer feed of described green ecological produced according to the following steps by the raw material getting described weight portion:

A) get bunge pricklyash leaf, Folium Pterocaryae, pomegranate leaf, walnut leaf, clean in the NaCl aqueous solution according to above-mentioned weight, dry; B) leaf dried and wheat bran are stirred after prepare 1 decile mother of vinegar cold water 100kg being diluted in whipping process and evenly spray (the mother of vinegar liquid after every 100kg dilution can process 1000 kilograms of leaves and rice hulls mixture).C) storage is held fermentor available cylinder, bucket, cement pit etc. and with plastic sheeting, fermentor is held in storage and covers airtight good (lucifuge), until fermentation maturity get final product (can smell wine, vinegar fragrance time).D) preparation of mother of vinegar: 1 decile mother of vinegar culture medium prescription: edible alcohol 3-4ml, glucose 0.4g, yeast extract 1g, calcium carbonate 1.5g, agar 1.5g, water 100g.Cultivate: cultivate after 2 days at 30 DEG C, Refrigerator store (4 DEG C).

2. the medicinal deer feed of green ecological according to claim 1, is characterized in that, one of meet the following conditions, be described fermenting-ripening:

A) fermentate is in yellow;

B) brown liquid is had to overflow;

C) volume ratio of fermentate decreases 2/3 to 1/2 before comparatively fermenting;

D) fragrance of wine, vinegar can be smelt.

E) possess sour, sweet, fragrant, soft, ripe.

3. the medicinal deer feed of green ecological according to claim 1, it is characterized in that, the fermentation time of described fermentate is 6 days to 15 days.

4. the medicinal deer feed of green ecological according to claim 1, is characterized in that, step a) and step b) and step c) described Chinese prickly ash is rutaceae green pepper and Chinese prickly ash; Described willow is the full section of Dilleniidae Salix; Described pomegranate tree is the full section of Punicaceae; Described walnut is the full section of juglans.

5. the method for the medicinal deer feed of the described green ecological of one of claim 1-4, is characterized in that, the ruminant domestic animal that can be used for feeding also is suitable for monogastric of feeding.