CN101400800B - 利用酶检测电极微阵列上结合事件的方法 - Google Patents
利用酶检测电极微阵列上结合事件的方法 Download PDFInfo
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- CN101400800B CN101400800B CN2006800426991A CN200680042699A CN101400800B CN 101400800 B CN101400800 B CN 101400800B CN 2006800426991 A CN2006800426991 A CN 2006800426991A CN 200680042699 A CN200680042699 A CN 200680042699A CN 101400800 B CN101400800 B CN 101400800B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Description
Claims (33)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/232,479 | 2005-09-21 | ||
US11/232,479 US20070231794A1 (en) | 2005-09-21 | 2005-09-21 | Process to detect binding events on an electrode microarray using enzymes |
PCT/US2006/036616 WO2008051196A2 (en) | 2005-09-21 | 2006-09-20 | Process to detect binding events on an electrode microarray using enzymes |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101400800A CN101400800A (zh) | 2009-04-01 |
CN101400800B true CN101400800B (zh) | 2013-03-27 |
Family
ID=38559551
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2006800426991A Active CN101400800B (zh) | 2005-09-21 | 2006-09-20 | 利用酶检测电极微阵列上结合事件的方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20070231794A1 (zh) |
EP (1) | EP1951913B1 (zh) |
JP (1) | JP2009510485A (zh) |
CN (1) | CN101400800B (zh) |
WO (1) | WO2008051196A2 (zh) |
Families Citing this family (34)
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US10539561B1 (en) * | 2001-08-30 | 2020-01-21 | Customarray, Inc. | Enzyme-amplified redox microarray detection process |
US20060102471A1 (en) | 2004-11-18 | 2006-05-18 | Karl Maurer | Electrode array device having an adsorbed porous reaction layer |
US20070034513A1 (en) | 2005-03-25 | 2007-02-15 | Combimatrix Corporation | Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array |
US9394167B2 (en) | 2005-04-15 | 2016-07-19 | Customarray, Inc. | Neutralization and containment of redox species produced by circumferential electrodes |
US20070065877A1 (en) | 2005-09-19 | 2007-03-22 | Combimatrix Corporation | Microarray having a base cleavable succinate linker |
US8855955B2 (en) * | 2005-09-29 | 2014-10-07 | Custom Array, Inc. | Process and apparatus for measuring binding events on a microarray of electrodes |
CN102308206A (zh) * | 2007-09-17 | 2012-01-04 | 红象牙有限责任公司 | 自作用信号产生检测设备和方法 |
ES2334745B1 (es) | 2008-07-11 | 2011-02-14 | Consejo Superior De Investigaciones Cientificas (Csic) | Dispositivo de lectura de microarrays de tipo electrico y reutilizable. |
WO2011009422A1 (de) * | 2009-07-21 | 2011-01-27 | Heiko Schwertner | Verfahren unter der verwendung von non impact druckmethoden zusammen mit druckflüssigkeiten, die aktive biologische moleküle enthalten, zur produktion von sensoren und komplexen analytischen systemen. |
US9927434B2 (en) | 2010-01-20 | 2018-03-27 | Customarray, Inc. | Multiplex microarray of serially deposited biomolecules on a microarray |
CN101880627B (zh) * | 2010-01-28 | 2012-10-10 | 湖南大学 | 同时检测两种木质素降解酶功能基因的生物传感器及其制备和检测方法及电化学检测系统 |
US9683258B2 (en) * | 2010-05-14 | 2017-06-20 | Sharp Laboratories Of America, Inc. | Efficient sensor for detecting and determining the concentrations of targets |
WO2012055060A1 (en) | 2010-10-28 | 2012-05-03 | Eth Zurich | Method for electrical detection of biomolecules by metal dissolution and assay kit therefore |
JP5795184B2 (ja) * | 2011-04-22 | 2015-10-14 | 国立大学法人 奈良先端科学技術大学院大学 | 被検物質の電気化学的検出方法 |
CN102253194B (zh) * | 2011-06-25 | 2013-08-28 | 南方医科大学 | 一种采用安培免疫传感器进行夹心免疫反应的二抗探针 |
WO2013040435A2 (en) * | 2011-09-16 | 2013-03-21 | Credo Biomedical Pte Ltd | Molecular diagnostic assay device and method of use |
WO2013119845A1 (en) | 2012-02-07 | 2013-08-15 | Vibrant Holdings, Llc | Substrates, peptide arrays, and methods |
CN102654475B (zh) * | 2012-03-23 | 2014-10-01 | 上海大学 | 检测过氧化氢的生物电化学传感器及其制备方法 |
US10006909B2 (en) | 2012-09-28 | 2018-06-26 | Vibrant Holdings, Llc | Methods, systems, and arrays for biomolecular analysis |
US10286376B2 (en) | 2012-11-14 | 2019-05-14 | Vibrant Holdings, Llc | Substrates, systems, and methods for array synthesis and biomolecular analysis |
JP5981667B2 (ja) | 2013-02-15 | 2016-08-31 | ヴィブラント ホールディングス リミテッド ライアビリティ カンパニー | 増幅された電気化学発光検出のための方法および組成物 |
WO2015033229A2 (en) * | 2013-09-04 | 2015-03-12 | Credo Biomedical Pte Ltd. | Assay test device, kit and method of using |
CN104698043A (zh) * | 2013-12-09 | 2015-06-10 | 重庆医科大学 | 检测大肠杆菌的dna电化学生物传感器及其制备方法 |
CN104614417B (zh) * | 2014-02-17 | 2016-10-19 | 安阳师范学院 | 一种用于糖蛋白检测的电化学方法 |
US10753940B2 (en) * | 2015-08-03 | 2020-08-25 | President And Fellows Of Harvard College | Enhanced electrochemical detection using nanoparticles and precipitation |
WO2018218250A2 (en) | 2017-05-26 | 2018-11-29 | Vibrant Holdings, Llc | Photoactive compounds and methods for biomolecule detection and sequencing |
TWI658268B (zh) * | 2017-11-29 | 2019-05-01 | 國立清華大學 | 血液檢測方法 |
WO2019203493A1 (ko) * | 2018-04-17 | 2019-10-24 | 한국화학연구원 | 멀티웰 전극 기반 바이오센서 |
JP7433300B2 (ja) * | 2018-05-17 | 2024-02-19 | レコグニション アナリティクス インコーポレイテッド | 酵素活性の直接電気測定用のデバイス、システムおよび方法 |
EP3824099A4 (en) * | 2018-12-13 | 2021-08-11 | Hewlett-Packard Development Company, L.P. | MULTIPLEX NUCLEIC ACID DETECTION |
BR112021023924A2 (pt) * | 2019-05-28 | 2022-02-01 | Adama Makhteshim Ltd | Sistemas e métodos de detecção de um organismo patogênico |
US20220403451A1 (en) * | 2019-10-31 | 2022-12-22 | President And Fellows Of Harvard College | On-chip assay strategy for the development of electrochemical readout for crispr-cas diagnostics |
JP2021143893A (ja) * | 2020-03-11 | 2021-09-24 | 国立大学法人九州大学 | 内分泌攪乱物質等の定量方法 |
GB202211527D0 (en) * | 2022-08-08 | 2022-09-21 | Univ Oxford Innovation Ltd | Electrochemical shotgun tagging assay with catalytic amplification |
Citations (2)
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US20040023258A1 (en) * | 2000-09-04 | 2004-02-05 | Fernando Patolsky | Method and system or detecting nucleic acids |
US20040072158A1 (en) * | 1997-03-18 | 2004-04-15 | Henkens Robert W. | Electrochemical detection of nucleic acid sequences |
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US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
JPH04204379A (ja) * | 1990-11-30 | 1992-07-24 | Hitachi Ltd | 生体成分分析法 |
US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
US5605662A (en) | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
US5846708A (en) * | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
ZA975891B (en) | 1996-07-05 | 1998-07-23 | Combimatrix Corp | Electrochemical solid phase synthesis of polymers |
JPH10253632A (ja) * | 1997-03-10 | 1998-09-25 | Nissui Pharm Co Ltd | 分析方法、キット及び装置 |
US6093302A (en) | 1998-01-05 | 2000-07-25 | Combimatrix Corporation | Electrochemical solid phase synthesis |
JPH11322799A (ja) * | 1998-05-13 | 1999-11-24 | Tosoh Corp | 抗体の固定化方法 |
EP1090286A1 (en) * | 1998-06-24 | 2001-04-11 | Therasense, Inc. | Multi-sensor array for electrochemical recognition of nucleotide sequences and methods |
CN1185492C (zh) * | 1999-03-15 | 2005-01-19 | 清华大学 | 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用 |
US6294392B1 (en) * | 1999-07-21 | 2001-09-25 | The Regents Of The University Of California | Spatially-encoded analyte detection |
JP2004538443A (ja) * | 2000-08-15 | 2004-12-24 | バイオフォース ナノサイエンシズ インコーポレイテッド | ナノスケール分子配列装置 |
US10539561B1 (en) * | 2001-08-30 | 2020-01-21 | Customarray, Inc. | Enzyme-amplified redox microarray detection process |
DE10228260A1 (de) * | 2002-06-25 | 2004-01-22 | Bayer Ag | Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe |
AU2003900285A0 (en) * | 2003-01-20 | 2003-02-06 | Universal Biosensors Pty Limited | Electrochemical detection method |
DE10311315A1 (de) * | 2003-03-14 | 2004-09-30 | Apibio Sas | Verfahren und Vorrichtung zur Detektion von Biomolekülen |
JP2005069836A (ja) * | 2003-08-22 | 2005-03-17 | Daikin Ind Ltd | 電極、タンパク測定装置及び酵素活性の測定方法 |
US7723099B2 (en) * | 2003-09-10 | 2010-05-25 | Abbott Point Of Care Inc. | Immunoassay device with immuno-reference electrode |
US20060160100A1 (en) * | 2005-01-19 | 2006-07-20 | Agency For Science, Technology And Research | Enzymatic electrochemical detection assay using protective monolayer and device therefor |
JP2005181350A (ja) * | 2005-03-22 | 2005-07-07 | Tohoku Techno Arch Co Ltd | 凸状領域を用いた分析方法 |
US10807805B2 (en) | 2013-05-17 | 2020-10-20 | Intelligrated Headquarters, Llc | Robotic carton unloader |
-
2005
- 2005-09-21 US US11/232,479 patent/US20070231794A1/en not_active Abandoned
-
2006
- 2006-09-20 EP EP06851884.4A patent/EP1951913B1/en active Active
- 2006-09-20 CN CN2006800426991A patent/CN101400800B/zh active Active
- 2006-09-20 WO PCT/US2006/036616 patent/WO2008051196A2/en active Application Filing
- 2006-09-20 JP JP2008541161A patent/JP2009510485A/ja active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040072158A1 (en) * | 1997-03-18 | 2004-04-15 | Henkens Robert W. | Electrochemical detection of nucleic acid sequences |
US20040023258A1 (en) * | 2000-09-04 | 2004-02-05 | Fernando Patolsky | Method and system or detecting nucleic acids |
Also Published As
Publication number | Publication date |
---|---|
JP2009510485A (ja) | 2009-03-12 |
CN101400800A (zh) | 2009-04-01 |
EP1951913A4 (en) | 2009-06-10 |
EP1951913B1 (en) | 2014-09-10 |
EP1951913A2 (en) | 2008-08-06 |
WO2008051196A2 (en) | 2008-05-02 |
WO2008051196A3 (en) | 2008-10-09 |
US20070231794A1 (en) | 2007-10-04 |
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Effective date of registration: 20170428 Address after: Washington State Patentee after: Carter Murray Co Ltd Address before: Washington State Patentee before: Combimatrix Corp. |
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Application publication date: 20090401 Assignee: NANJINGJINSIRUI SCIENCE & TECHNOLOGY BIOLOGY Corp. Assignor: Carter Murray Co.,Ltd. Contract record no.: X2022990000126 Denomination of invention: Method for detecting binding events on electrode microarray by enzyme Granted publication date: 20130327 License type: Exclusive License Record date: 20220307 |