CN101357112A - Acne-removing additive - Google Patents
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Abstract
The invention discloses an additive with anti-acne efficacy. The additive with anti-acne efficacy of the invention is obtained by extracting raw materials such as salvia miltiorrhiza, angelica dahurica and rhubarb by organic solvent. The parts by weight of salvia miltiorrhiza, angelica dahurica and rhubarb are 11-55 parts, 2-22 parts and 1-27 parts. The organic solvent is absolute ethyl alcohol. The additive with anti-acne efficacy of the invention can play tremendous role in the production of functional cosmetics such as anti-acne, anti-aging, whitening, etc.
Description
Technical field
The present invention relates to a kind of acne-removing additive, particularly relate to screening and preparation acne-removing additive from Chinese herbal medicine.
Background technology
Acne (Acne) is an acne vulgaris, be commonly called as " acne ", " comedo ", " whelk " or " wine pimple ", being common hair follicle, the sebaceous gland chronic inflammatory diseases due to one kind of multiple combined factors effects, is the more general dermatosis that most of youth of both sexes existed in adolescence.The teenager of the nearest Epidemiological study demonstration of doing 44.5% of China suffers from acne.It is not only a kind of physical disease, also is a kind of psychosomatic disease that can not be ignored, and is all influential to the patient at aspects such as social activity, psychology, emotions.
Acne occurs in face, also can occur in thoracic dorsal top and shoulder, also betides the red pimple at other positions once in a while.The vigorous position of sebaceous gland is heavy, the position that smegma is few light (all skin of socket of the eye never involves).The patient is an oily skin mostly, and beginning mostly is homeliness type greatly, tuberosity, pustule, abscess, sinus tract or cicatrix can occur later on.The course of disease is long, and easily repeatedly, many no conscious symptons, then can cause pain and touch painful when obvious as inflammation.Studies confirm that the pathogeny of acne (comedo) and the inducement mechanism that aggravates disease, mainly be divided into androgen to the unusual adjusting of sebum secretion, horn cell the hyperplasia especially keratinocyte of basal layer break up unusually with the hair follicle horn cell, a large amount of continuous processs of breeding and inflammatory reactions that cause thus and immunoreation etc. fast of the relevant pathogen of acne.
By above-mentioned analysis, though we think that the organism endocrine imbalance is the source that acne takes place, a large amount of breedings fast of the relevant pathogen of acne then are the key links that acne takes place.Therefore,, diminish inflammation on the other hand as far as possible and react and immunoreation, then can reach the effect of efficient anti-acne if one side suppresses a large amount of breedings fast of the relevant pathogen of acne.The method of treatment acne mainly contains western medicine therapy, tcm therapy, three aspects of the combined therapy of Chinese and Western medicine at present, and medicine mainly contains broad ectrum antibiotic, antiandrogen, tretinoin, estrogen, corticosteroid hormone, Chinese medicine compound etc.Mainly by antibiotic therapy, curative effect is very fast for western medicine therapy, and relevant pathogen develops immunity to drugs but it makes acne easily, is difficult to radical cure, and certain side effect is arranged, and be easy to generate dependency; Tcm therapy mainly is the conditioning endocrine, and its curative effect is slower, and the combination of Chinese and Western medicine reports that at present therapeutic effect is relatively good, and curative effect is short, but is Drug therapy mostly, needs to take orally, and the external kind is less.Acne treatment new drug just under development has protein synthesis inhibitor, DNA topoisomerase enzyme inhibitor, protein 50 ribosomal subunit's inhibitor, oxidant, androgen antagonist, cell wall synthetic inhibitor, 5 inhibitor, antibiotic etc. at present, but the acne-removing additive kind and the negligible amounts of used for cosmetic, the acne-removing additive of natural origin are then still less.
In the epoch that " green " notion is more and more popularized, people more and more pay attention to for the additive of anti-acne efficiently of natural harmless safety, based on the huge kind of plant, quantity, and have multifarious characteristics of the material that is rich in, the research of China Chinese medicine utility theory is with a long history, and therefore Tremendous achievements develop the acne-eliminating cosmetic safety, non-stimulated of Chinese medicine compound, adapting to the cosmetics trend, also is the first-selection source of the natural acne-removing additive of screening.
Radix Et Rhizoma Rhei is the dry root of polygonum rheum palmatum Rheum palmatum L..Hardship, cold.Return spleen, stomach, large intestine, liver, pericardium channel.Cure mainly purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation, the excess-heat constipation, stagnant stomachache, dysentery is not well, jaundice due to damp-heat, heat in blood is told nosebleed, conjunctival congestion, pharyngeal swelling, abdominalgia with intestinal abscess, carbuncle furuncle, blood stasis amenorrhea, traumatic injury, external treatment burn due to hot liquid or fire; Upper gastrointestinal hemorrhage.Experimental results show that emodin all can suppress the biosynthesis of the white three rare B of inflammatory mediator in vivo and in vitro under the condition, is the inhibitor of arachidonic acid lipoxygenase.
Radix Scutellariae is the dry root of labiate Radix Scutellariae Scutellaria baicalensis Georgi.Hardship, cold.Return lung, gallbladder, spleen, large intestine, small intestine meridian.Cure mainly heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive.The Radix Scutellariae external can suppress the generation of dihydrotestosterone in the sebaceous gland.
Radix Salviae Miltiorrhizae is the dry root and rhizome of labiate Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bge..Hardship is slightly cold.GUIXIN, Liver Channel.Cure mainly stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, relieving restlessness clears away heart-fire.Be used for menoxenia, the amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia; Hepatosplenomegaly, angina pectoris.The main component TANSHINONES of Radix Salviae Miltiorrhizae has antiandrogen effect and gentle estrogen-like effects.
Flos Lonicerae is the flower that caprifoliaceae plant Radix Ophiopogonis Lonicera japonica Thunb. drying is just opened.Sweet, cold.Return lung, the heart, stomach warp.Thermal detoxification, wind-heat dissipating.Be used for the carbuncle furuncle, sore throat, erysipelas, warm blood fulminant dysentery, anemopyretic cold, epidemic febrile disease heating.According to the pharmaceutical chemistry analysis, Flos Lonicerae contains luteolin, inositol, Saponin, tannin etc.
Cortex Phellodendri is the dry bark of rutaceae wampee Phellodendron chinense Schneid..Hardship, cold.Return kidney, urinary bladder channel.Heat clearing and damp drying, pathogenic fire purging are removed and are steamed, and skin ulcer is treated in detoxifcation.Be used for damp-heat dysentery, jaundice, leukorrhagia, pyretic stranguria, beriberi, flaccidity { is warded off }, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema pruritus.The stir-baked CORTEX PHELLODENDRI with salt solution nourishing YIN to lower pathogenic fire.
The Radix Angelicae Dahuricae: the Radix Angelicae Dahuricae is the root of the Radix Angelicae Dahuricae Angelica dahurica Benth.et Hook. of samphire Xingan.Suffering, temperature.Attach to the lung and stomach meridians.Function cures mainly expelling wind and cold, understand things pain-stopping, detumescence and apocenosis, dampness leukorrhagia stopping.That this product has is analgesic, analgesia, antiinflammatory action.Angelicotoxin has the effect of excited oblongata vasomotor center, respiratory center, vagus nerve and spinal cord in a small amount; The blood pressure that can raise makes slack-off, the exaggerated respiration of pulse, causes the sialorrhea vomiting, and heavy dose can cause that spasm benumbs then.Contained furan tonkabean compounds has the heliosensitivity effect, and smooth muscle is had spasmolysis.The effect of the antibacterial of inhibition and fungus is still arranged in addition.
Cortex Magnoliae Officinalis: Cortex Magnoliae Officinalis is the outer peel of immature drying of rutaceae Huazhou Fructus Citri grandis Citrus grandis Tomentosa.Hot, bitter, temperature.Return lung, spleen channel.Cure mainly: taste are deficient, and vomiting due to accumulation of phlegm is contrary, inappetence, and distension before the abdomen, the flatulence heart is vexed, inappetence, trouble is not healed for a long time, regurgitation, drop, abdominal pain due to acute vomiting and diarrhea, chronic dysentery, large intestine is dry and hard, Cloudy Urine, menstruation is obstructed.
Summary of the invention
The purpose of this invention is to provide a kind of acne-removing additive.
Acne-removing additive provided by the present invention is to extract from the raw material that comprises Radix Salviae Miltiorrhizae, the Radix Angelicae Dahuricae and Radix Et Rhizoma Rhei with organic solvent to obtain;
The parts by weight of Radix Salviae Miltiorrhizae are in the described raw material: 11-55 part; The parts by weight of the Radix Angelicae Dahuricae are: 2-22 part; The parts by weight of Radix Et Rhizoma Rhei are: 1-27 part;
Described organic solvent is that methanol, volumn concentration are ethanol water or the dehydrated alcohol more than 70%.
The parts by weight of described Radix Salviae Miltiorrhizae are specially 55 parts; The parts by weight of the described Radix Angelicae Dahuricae are specially 22 parts; The parts by weight of described Radix Et Rhizoma Rhei are specially 10 parts.
The relevant pathogen of described acne can be at least a in following five kinds of pathogen: Staphylococcyssaccharolyticus, Arcanobacterium pyogenes, Fusobacterium nucleaturn ss polymorphum, Clostridium absonum and Staphulococcua aureus.
The raw material of acne-removing additive of the present invention also can comprise Radix Scutellariae or Cortex Magnoliae Officinalis;
The relevant pathogen of described acne is S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion; Radix Angelicae Dahuricae 2-22 weight portion; Radix Et Rhizoma Rhei 1-27 weight portion; Radix Scutellariae 2-41 weight portion;
The relevant pathogen of described acne is S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion; Radix Angelicae Dahuricae 2-22 weight portion; Radix Et Rhizoma Rhei 1-27 weight portion; Cortex Magnoliae Officinalis 1-68 weight portion.
The raw material of acne-removing additive of the present invention also can comprise the Radix Scutellariae magnolia obovata;
The relevant pathogen of described acne is S.saccharolyticus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 22-55 weight portion, Radix Et Rhizoma Rhei 10-27 weight portion, Radix Scutellariae 3-41 weight portion, Cortex Magnoliae Officinalis 1-32 weight portion, Radix Angelicae Dahuricae 3-22 weight portion;
The relevant pathogen of described acne is A.pyogenes; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 22-55 weight portion, Radix Et Rhizoma Rhei 3-27 weight portion, Radix Scutellariae 7-41 weight portion, Cortex Magnoliae Officinalis 1-45 weight portion, Radix Angelicae Dahuricae 2-22 weight portion;
The relevant pathogen of described acne is F.nucleaturn; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 32-55 weight portion, Radix Et Rhizoma Rhei 1-19 weight portion, Radix Scutellariae 3-32 weight portion, Cortex Magnoliae Officinalis 6-32 weight portion, Radix Angelicae Dahuricae 14-22 weight portion;
The relevant pathogen of described acne is C.absonum; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-27 weight portion, Radix Et Rhizoma Rhei 3-27 weight portion, Radix Scutellariae 2-41 weight portion, Cortex Magnoliae Officinalis 1-68 weight portion, Radix Angelicae Dahuricae 2-10 weight portion;
The relevant pathogen of described acne is S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion, Radix Et Rhizoma Rhei 9-27 weight portion, Radix Scutellariae 2-41 weight portion, Cortex Magnoliae Officinalis 1-68 weight portion, Radix Angelicae Dahuricae 9-22 weight portion;
The parts by weight of described Radix Salviae Miltiorrhizae are specially 55 parts; The parts by weight of the described Radix Angelicae Dahuricae are specially 22 parts; The parts by weight of described Radix Et Rhizoma Rhei are specially 10 parts; The parts by weight of described Radix Scutellariae are specially 7 parts; The parts by weight of described Cortex Magnoliae Officinalis are specially 6 parts.
The present invention obtains in separation and purification on the basis of the relevant pathogen of acne, to the relevant pathogen fungistatic effect of acne, obtains effectively suppressing the prescription of the relevant pathogen of acne by relatively more existing Chinese herbal medicine.Acne-removing additive of the present invention can effectively delay the relevant pathogen of acne, can be used in the acne-eliminating cosmetic, is the low green cosmetics functional additive that stimulates pure natural.
Description of drawings
Fig. 1 separates the position of the relevant pathogen of acne for patients with acne skin
Fig. 2 is the relevant pathogen colony characteristics of acne that separates the purification gained from the patient skin acne
The Gram result of the relevant pathogen of Fig. 3 acne
The specific embodiment
Separation, purification and the evaluation of embodiment 1, the relevant pathogen of acne
1. the separation of the relevant pathogen of acne
The alcohol disinfecting of skin surface (accompanying drawing 1) with patients with acne with 75%, after puncturing acne with sterile needle, the acne content is extruded, put into the centrifuge tube that sterilized water is housed, it is fully vibrated it is solved homogeneously in the sterilized water, be diluted to the bacterium liquid of variable concentrations then according to 10 times gradient ratio, bed board is put into 37 ℃ of cultivations of anaerobism incubator immediately then.
The acne content that extracts on one's body from patients with acne was placed on 37 ℃ of anaerobism cultivations after 3 days, will separate the pathogen that obtains according to the diversity of colonial morphology and be divided into four kinds.With of the Concentraton gradient dilution of one single bacterium colony according to 10 times, continue to be placed in 37 ℃ of anaerobism incubators and cultivated 3 days, promptly obtain the relevant pathogen of 4 kinds of acnes of purification, difference called after W1, W2, W3 and W4 (accompanying drawing 2).
2. acne pathogen Gram is identified
Gram: the conventional steps that adopts Gram staining method, be smear → fixing → 1 minute → washing of crystallization purple dyeing and blot → 1 minute → washing of iodine liquid mordant dyeing and blot → decolouring of 95% ethanol with absorbent paper with absorbent paper, when ethanol purple do not occur, stop (approximately half a minute) → sarranine redyes about 3 minutes → washing and with absorbent paper blot → dry → under the microscope lens,oil immersion, observe, it is gram positive bacteria that thalline shows hepatic; What thalline showed redness is gram negative bacteria.
The relevant pathogen of separate of acquisition acne is carried out Gram, and the microscope lens,oil immersion is observed down, and four kinds of pathogen all show bluish violets, are Gram-positive (accompanying drawing 3) so conclude four kinds of bacterium.
3. acne pathogen Biolog microbial identification
Biolog microbial identification step: at first the anaerobe of needs being identified with the BUA culture medium carries out amplification culture.Power-on is got the test tube that inoculation liquid is housed of not uncapping, and wipes clean tube wall, puts into transmissometer, and with turbidity standard pipe (AN) check, reading is 65% ± 2% then.In Biohazard Safety Equipment, in the rolling gently of bacterium colony surface, lawn is got in the inoculation liquid with cotton swab.In vitro dry position, the top of wall inoculation liquid liquid level rotates the extruding cotton swab, with cotton swab and pipe friction, lawn is disperseed as far as possible.Move up and down cotton swab then, dispersive thalline and inoculation liquid are fully mixed the inoculation liquid that forms homogeneous, do not have caking.Action should be slight in the time of blended, enters inoculation liquid in order to avoid bring too many bubble into, and turbidity is read in influence.Corresponding number on the volume.Bacteria suspension is poured into reservoir, select pipettor corresponding program (every hole adds 100 μ l bacteria suspensions), the pipettor head is placed on the pipettor, draw bacteria suspension, add the AN identification plate, cover lid.Spread wet paper towel or wetted filter paper in the bottom, above identification plate was placed on, cultivation temperature was 37 ℃, cultivated 20-24h, and for growth fraction anaerobe more slowly, incubation time can increase to 2 days.
Utilize Microstation software to read the data of identification plate.Readout instrument carries out dual wavelength (490nm measures orange red, and 750nm measures turbidity) automatically to be measured, and software is divided the yin and yang attribute reaction according to threshold value, and final qualification result is as shown in table 1.
The Biolog microbial identification result of the relevant pathogen of four kinds of acnes of table 1
Embodiment 2, Chinese herbal medicine extract detect the inhibition ability of the relevant pathogen of acne
The preparation of Chinese herbal medicine extract: 7 kinds of Chinese herbal medicine pulverize separately are also crossed 120 mesh sieves with pulverizer.The Chinese herbal medicine powder mixture 30g that gets after the pulverizing places the ground triangular flask, adds the 300mL dehydrated alcohol, bathes in the shaking table (55 ℃ of vibrations at full warm air, 150rpm) after 1 hour, use filter paper filtering, collect filtrate, precipitation adds the 300mL dehydrated alcohol again, repeats above step 2 time.After 3 filtrates are merged, quantitatively to 900mL, under 50 ℃ condition, be concentrated into driedly then, add the 90ml dehydrated alcohol then and dissolve, promptly obtain Chinese herbal medicine extract identified as samples to be determined with dehydrated alcohol.
Bacteriostasis detects: the S.saccharolyticus that this experiment is separated to example 1, A.pyogenes, the relevant relevant pathogen staphylococcus aureus with the existing acne of this laboratory of pathogen (Staphulococcua aureus) of four kinds of acnes of F.nucleaturn, C.absonum are as indicator bacteria.Draw the 0.1ml bacteria suspension respectively on corresponding solid medium with aseptic rifle head, being coated in media surface equably with aseptic glass triangle bamboo rake makes and contains the bacterium flat board, with (121 ℃ of the bacterium of going out of aseptic nipper gripping 6mm diameter, 21min) filter paper is placed on the empty culture dish of the bacterium of going out, drip corresponding sample, treat to be placed on the culture dish that contains bacterium after filter paper is done, each different sample is done three repetitions, after anaerobe is put into 37 ℃ anaerobism incubator constant temperature culture 48h, measure antibacterial circle diameter.
Respectively the bacteriostasis of 7 kinds of Chinese herbal medicine extracts is measured according to the method described above, three repetitions are established in experiment, and three times multiple meansigma methods is as shown in table 2.Relatively the different Chinese medicine extract of 7 flavors to the inhibition effect of the relevant anaerobe of acne as can be seen, it is respectively Radix Et Rhizoma Rhei, Radix Scutellariae, Radix Salviae Miltiorrhizae magnolia obovata that S.saccharolyticus, A.pyogenes, five kinds of relevant pathogen of acne of F.nucleaturn, C.absonum and S.aureus are all had the Chinese herbal medicine of bacteriostasis, and F.nucleaturn is had the Chinese herbal medicine of bacteriostasis also have the Radix Angelicae Dahuricae except that above 4 kinds.Wherein the inhibition ability of Radix Et Rhizoma Rhei and Radix Salviae Miltiorrhizae is relative stronger, and antibacterial circle diameter has all surpassed 10mm, and the inhibition zone of Radix Et Rhizoma Rhei is 20mm especially, has shown outstanding bacteriostasis.
The different Chinese herbal medicine extracts of table 2 compare the bacteriostasis of the relevant pathogen of acne
Embodiment 3, with the mensuration of the acne-removing additive bacteriostasis of 5 kinds of Chinese herbal medicine extracts preparation
On the basis of embodiment 2, we select five kinds of Chinese herbal medicine of Radix Salviae Miltiorrhizae, Radix Et Rhizoma Rhei, Radix Scutellariae, the Radix Angelicae Dahuricae, Cortex Magnoliae Officinalis as the further Chinese herbal medicine of compound recipes, by the DPS software design raw material compound recipe of 12 acne-removing additives.In 12 acne-removing additives, every kind of proportion of raw materials is as shown in table 3.
The raw material of table 3 acne-removing additive is formed (weight: g)
Proportioning according to table 3 takes by weighing various Chinese herbal medicine, with pulverizer various Chinese herbal medicine pulverize separately is also crossed 120 mesh sieves.The Chinese herbal medicine powder mixture 10g that gets after the pulverizing places the ground triangular flask, adds the 100mL dehydrated alcohol, bathes in the shaking table (55 ℃ of vibrations at full warm air, 150rpm) after 1 hour, use filter paper filtering, collect filtrate, precipitation adds the 100mL dehydrated alcohol again, repeats above step 2 time.After 3 filtrates merging, quantitatively to 300mL, under 50 ℃ condition, concentrate 250ml with dehydrated alcohol then, be acne-removing additive 1-12.
Measure the bacteriostasis of acne-removing additive 1-12 to S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus according to the method among the embodiment 2, the result is as shown in table 4.Table 4 shows among the acne-removing additive 1-12, three acne-removing additives the strongest to the S.saccharolyticus bacteriostasis are acne-removing additive 1, acne-removing additive 3 and acne-removing additive 5, are respectively specific to each component effective range of acne-removing additive raw material: Radix Salviae Miltiorrhizae 22-55g, Radix Et Rhizoma Rhei 10-27g, Radix Scutellariae 3-41g, Cortex Magnoliae Officinalis 1-32g, Radix Angelicae Dahuricae 3-22g; Three acne-removing additives the strongest to the A.pyogenes bacteriostasis are acne-removing additive 1, acne-removing additive 4 and acne-removing additive 5, are respectively specific to each component effective range of acne-removing additive raw material: Radix Salviae Miltiorrhizae 22-55g, Radix Et Rhizoma Rhei 3-27g, Radix Scutellariae 7-41g, Cortex Magnoliae Officinalis 1-45g, Radix Angelicae Dahuricae 2-22g; Three acne-removing additives the strongest to the F.nucleaturn bacteriostasis are acne-removing additive 1, acne-removing additive 2 and acne-removing additive 3, are respectively specific to each component effective range of acne-removing additive raw material: Radix Salviae Miltiorrhizae 32-55g, Radix Et Rhizoma Rhei 1-19g, Radix Scutellariae 3-32g, Cortex Magnoliae Officinalis 6-32g, Radix Angelicae Dahuricae 14-22g; Three acne-removing additives the strongest to the C.absonum bacteriostasis are acne-removing additive 4, acne-removing additive 5 and acne-removing additive 8, are respectively specific to each component effective range of acne-removing additive raw material: Radix Salviae Miltiorrhizae 11-27g, Radix Et Rhizoma Rhei 3-27g, Radix Scutellariae 2-41g, Cortex Magnoliae Officinalis 1-68g, Radix Angelicae Dahuricae 2-10g; Three acne-removing additives the strongest to the S.aureus bacteriostasis are acne-removing additive 1, acne-removing additive 5 and acne-removing additive 8, are respectively specific to each component effective range of acne-removing additive raw material: Radix Salviae Miltiorrhizae 11-55g, Radix Et Rhizoma Rhei 9-27g, Radix Scutellariae 2-41g, Cortex Magnoliae Officinalis 1-68g, Radix Angelicae Dahuricae 9-22g.Therefore, the effective range of various herbal components that the relevant pathogen of five kinds of acnes is all had an acne-removing additive compound recipe of best fungistatic effect is: Radix Salviae Miltiorrhizae 11-55g, Radix Et Rhizoma Rhei 1-27g, Radix Scutellariae 2-41g, Cortex Magnoliae Officinalis 1-68g, Radix Angelicae Dahuricae 2-22g.
The antibacterial circle diameter unit of table 4 acne-removing additive: mm
By more as can be seen, acne-removing additive 1 is the acne-removing additive prescription the strongest to the bacteriostasis of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus, and its raw material consists of: Radix Salviae Miltiorrhizae 55g, Radix Et Rhizoma Rhei 10g, Radix Scutellariae 7g, Cortex Magnoliae Officinalis 6g, Radix Angelicae Dahuricae 22g.The antibacterial circle diameter of acne-removing additive 1 couple of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus is respectively 19.07,19.13,13.43,6.68 and 11.92 millimeters.Therefore, the best acne-removing additive that screens for the present invention of acne-removing additive 1.
Embodiment 4, with the mensuration of the acne-removing additive bacteriostasis of 3 kinds of Chinese herbal medicine extracts preparation
With Radix Salviae Miltiorrhizae, Radix Et Rhizoma Rhei and the Radix Angelicae Dahuricae is raw material, has designed the raw material compound recipe of 4 acne-removing additives.In 4 acne-removing additives, every kind of proportion of raw materials is as shown in table 5.
Proportioning according to table 5 takes by weighing various Chinese herbal medicine, with pulverizer various Chinese herbal medicine pulverize separately is also crossed 120 mesh sieves.The Chinese herbal medicine powder mixture 10g that gets after the pulverizing places the ground triangular flask, adds the 100mL dehydrated alcohol, bathes in the shaking table (55 ℃ of vibrations at full warm air, 150rpm) after 1 hour, use filter paper filtering, collect filtrate, precipitation adds the 100mL dehydrated alcohol again, repeats above step 2 time.After 3 filtrates merging, quantitatively to 300mL, under 50 ℃ condition, concentrate 250ml with dehydrated alcohol then, be acne-removing additive 13-16.
The raw material of table 5 acne-removing additive is formed (weight: g)
Bacteriostasis detects: the S.saccharolyticus that this experiment is separated to example 1, A.pyogenes, the relevant relevant pathogen staphylococcus aureus with the existing acne of this laboratory of pathogen (Staphulococcua aureus) of four kinds of acnes of F.nucleaturn, C.absonum are as indicator bacteria.Draw 12.5 μ L bacteria suspensions respectively on corresponding solid medium with aseptic rifle head, being coated in media surface equably with aseptic glass triangle bamboo rake makes and contains the bacterium flat board, with (121 ℃ of the bacterium of going out of aseptic nipper gripping 6mm diameter, 21min) filter paper is placed on the empty culture dish of the bacterium of going out, drip corresponding sample, treat to be placed on the culture dish that contains bacterium after filter paper is done, each different sample is done three repetitions, after anaerobe is put into 37 ℃ anaerobism incubator constant temperature culture 48h, measure antibacterial circle diameter.Acne-removing additive 13-16 is to the bacteriostasis of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus, and the result is as shown in table 6.Table 6 shows among the acne-removing additive 13-16, to the bacteriostasis of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus the strongest be acne-removing additive 15.Each component of this acne-removing additive raw material is respectively: Radix Salviae Miltiorrhizae 55g, Radix Angelicae Dahuricae 22g, Radix Et Rhizoma Rhei 10g.The antibacterial circle diameter of this acne-removing additive 15 couples of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus is respectively 1.18,1.95,4.97,6.35 and 1.73 millimeters.
The antibacterial circle diameter unit of table 6 acne-removing additive: mm
Embodiment 5, with the mensuration of the acne-removing additive bacteriostasis of 4 kinds of Chinese herbal medicine extracts preparation
On the basis of the acne-removing additive 15 for preparing with Radix Salviae Miltiorrhizae, the Radix Angelicae Dahuricae and three kinds of Chinese herbal medicine of Radix Et Rhizoma Rhei that embodiment 3 filters out, investigated any one or two kinds of in Radix Scutellariae, the Cortex Magnoliae Officinalis and mixed its extract bacteriostatic ability of back with acne-removing additive 15, designed the raw material compound recipe of 3 acne-removing additives as auxiliary material.In 3 acne-removing additives, every kind of proportion of raw materials is as shown in table 7.
Proportioning according to table 7 takes by weighing various Chinese herbal medicine, with pulverizer various Chinese herbal medicine pulverize separately is also crossed 120 mesh sieves.The Chinese herbal medicine powder mixture 10g that gets after the pulverizing places the ground triangular flask, adds the 100mL dehydrated alcohol, bathes in the shaking table (55 ℃ of vibrations at full warm air, 150rpm) after 1 hour, use filter paper filtering, collect filtrate, precipitation adds the 100mL dehydrated alcohol again, repeats above step 2 time.After 3 filtrates merging, quantitatively to 300mL, under 50 ℃ condition, concentrate 250ml with dehydrated alcohol then, be acne-removing additive 17-19.
The raw material of table 7 acne-removing additive is formed (weight: g)
Measure the bacteriostasis of acne-removing additive 17-19 to S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus according to the method among the embodiment 4, the result is shown in 8.Table 8 shows on the basis of acne-removing additive 15, increases Radix Scutellariae separately and is respectively 0.25,3.18,6.32,7.37 and 2.27 millimeter as the antibacterial circle diameter of acne-removing additive 17 couples of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and the S.aureus of auxiliary material.Bacteriostasis than acne-removing additive 15 couples of A.pyogenes, F.nucleaturn, C.absonum and S.aureus has improved 63.08,27.16,6.06 and 31.21% respectively, but the bacteriostasis of S.saccharolyticus has been descended 78.81%.
Use Radix Salviae Miltiorrhizae what embodiment 3 filtered out, on the basis of the acne-removing additive 15 of three kinds of Chinese herbal medicine preparations of the Radix Angelicae Dahuricae and Radix Et Rhizoma Rhei, increase Cortex Magnoliae Officinalis 18 couples of S.saccharolyticus of acne-removing additive separately as auxiliary material, A.pyogenes, F.nucleaturn, the antibacterial circle diameter of C.absonum and S.aureus is respectively 0.13,2.37,6.66,7.65 and 2.28 millimeters, than 15 couples of A.pyogenes of acne-removing additive, F.nucleaturn, the bacteriostasis of C.absonum and S.aureus has improved 21.54 respectively, 34.00,20.47 and 31.79%, but the bacteriostasis of S.saccharolyticus has been descended 88.98%.
On the basis of acne-removing additive 17, increase the acne-removing additive 19 of Cortex Magnoliae Officinalis again, the antibacterial circle diameter of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus is respectively 1.30,4.66,8.10,8.23 and 3.22 millimeters as auxiliary material.Bacteriostasis than acne-removing additive 17 couples of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus has improved 420.00,46.54,28.16,11.67 and 41.85% respectively.
On the basis of acne-removing additive 18, increase the acne-removing additive 19 of Radix Scutellariae again, the antibacterial circle diameter of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus is respectively 1.30,4.66,8.10,8.23 and 3.22 millimeters as auxiliary material.Bacteriostasis than acne-removing additive 18 couples of S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus has then improved 900.00,96.62,21.62,7.58 and 41.23% respectively.
The antibacterial circle diameter unit of table 8 acne-removing additive: mm
Claims (10)
1, a kind of acne-removing additive is to extract from the raw material that comprises Radix Salviae Miltiorrhizae, the Radix Angelicae Dahuricae and Radix Et Rhizoma Rhei with organic solvent to obtain;
The parts by weight of Radix Salviae Miltiorrhizae are in the described raw material: 11-55 part; The parts by weight of the Radix Angelicae Dahuricae are: 2-22 part; The parts by weight of Radix Et Rhizoma Rhei are: 1-27 part;
Described organic solvent is that methanol, volumn concentration are ethanol water or the dehydrated alcohol more than 70%.
2, acne-removing additive according to claim 1 is characterized in that: described organic solvent is a dehydrated alcohol.
3, acne-removing additive according to claim 2 is characterized in that: the parts by weight of described Radix Salviae Miltiorrhizae are specially 55 parts; The parts by weight of the described Radix Angelicae Dahuricae are specially 22 parts; The parts by weight of described Radix Et Rhizoma Rhei are specially 10 parts.
4, according to claim 1,2 or 3 described acne-removing additives, it is characterized in that: the relevant pathogen of described acne can be at least a in following five kinds of pathogen: Staphylococcys saccharolyticus, Arcanobacterium pyogenes, Fusobacterium nucleaturn ss polymorphum, Clostridium absonum and Staphulococcua aureus.
5, according to arbitrary described acne-removing additive among the claim 1-4, it is characterized in that: raw material also can comprise Radix Scutellariae;
The relevant pathogen of described acne is S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion; Radix Angelicae Dahuricae 2-22 weight portion; Radix Et Rhizoma Rhei 1-27 weight portion; Radix Scutellariae 2-41 weight portion.
6, acne-removing additive according to claim 5 is characterized in that: also comprise Cortex Magnoliae Officinalis in the described raw material;
The relevant pathogen of described acne is S.saccharolyticus, A.pyogenes, F.nucleaturn, C.absonum and S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion; Radix Angelicae Dahuricae 2-22 weight portion; Radix Et Rhizoma Rhei 1-27 weight portion; Cortex Magnoliae Officinalis 1-68 weight portion.
7, according to arbitrary described acne-removing additive among the claim 1-4, it is characterized in that: raw material also can comprise the Radix Scutellariae magnolia obovata;
The relevant pathogen of described acne is S.saccharolyticus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 22-55 weight portion, Radix Et Rhizoma Rhei 10-27 weight portion, Radix Scutellariae 3-41 weight portion, Cortex Magnoliae Officinalis 1-32 weight portion, Radix Angelicae Dahuricae 3-22 weight portion;
The relevant pathogen of described acne is A.pyogenes; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 22-55 weight portion, Radix Et Rhizoma Rhei 3-27 weight portion, Radix Scutellariae 7-41 weight portion, Cortex Magnoliae Officinalis 1-45 weight portion, Radix Angelicae Dahuricae 2-22 weight portion;
The relevant pathogen of described acne is F.nucleaturn; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 32-55 weight portion, Radix Et Rhizoma Rhei 1-19 weight portion, Radix Scutellariae 3-32 weight portion, Cortex Magnoliae Officinalis 6-32 weight portion, Radix Angelicae Dahuricae 14-22 weight portion;
The relevant pathogen of described acne is C.absonum; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-27 weight portion, Radix Et Rhizoma Rhei 3-27 weight portion, Radix Scutellariae 2-41 weight portion, Cortex Magnoliae Officinalis 1-68 weight portion, Radix Angelicae Dahuricae 2-10 weight portion;
The relevant pathogen of described acne is S.aureus; The raw material of described acne-removing additive consists of: Radix Salviae Miltiorrhizae 11-55 weight portion, Radix Et Rhizoma Rhei 9-27 weight portion, Radix Scutellariae 2-41 weight portion, Cortex Magnoliae Officinalis 1-68 weight portion, Radix Angelicae Dahuricae 9-22 weight portion.
8, acne-removing additive according to claim 7 is characterized in that: the parts by weight of described Radix Salviae Miltiorrhizae are specially 55 parts; The parts by weight of the described Radix Angelicae Dahuricae are specially 22 parts; The parts by weight of described Radix Et Rhizoma Rhei are specially 10 parts; The parts by weight of described Radix Scutellariae are specially 7 parts; The parts by weight of described Cortex Magnoliae Officinalis are specially 6 parts.
9, the application of arbitrary described acne-removing additive in the preparation cosmetics among the claim 1-8.
10, a kind of is the cosmetics of active component preparation with arbitrary described acne-removing additive among the claim 1-8.
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| CNA2008101352144A CN101357112A (en) | 2008-08-05 | 2008-08-05 | Acne-removing additive |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101670000A (en) * | 2009-10-09 | 2010-03-17 | 北京工商大学 | Compound acne-removing Chinese herbal extract and preparation method therefor |
| CN101810556A (en) * | 2010-04-13 | 2010-08-25 | 上海相宜本草化妆品有限公司 | Combination of traditional Chinese medicine extractive with function of anti-skin aging, preparation and use |
| CN103432244A (en) * | 2013-09-11 | 2013-12-11 | 余红波 | Composition having acne removing function |
| CN103566137A (en) * | 2012-11-22 | 2014-02-12 | 冯兆章 | Chinese herbal medicine formula for removing acne |
| CN109045139A (en) * | 2018-11-01 | 2018-12-21 | 苏州卫生职业技术学院 | A kind of Chinese medicine composition and preparation method thereof inhibiting propionibacterium acnes |
| CN110237185A (en) * | 2019-07-04 | 2019-09-17 | 李菊莲 | A kind of face mask of traditional Chinese medicine for treating acne |
| CN111494598A (en) * | 2020-06-02 | 2020-08-07 | 郭宝云 | Oral and external traditional Chinese medicine for treating liver-stagnation and spleen-deficiency type acne and preparation method thereof |
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2008
- 2008-08-05 CN CNA2008101352144A patent/CN101357112A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101670000A (en) * | 2009-10-09 | 2010-03-17 | 北京工商大学 | Compound acne-removing Chinese herbal extract and preparation method therefor |
| CN101670000B (en) * | 2009-10-09 | 2012-11-14 | 北京工商大学 | Compound acne-removing Chinese herbal extract and preparation method therefor |
| CN101810556A (en) * | 2010-04-13 | 2010-08-25 | 上海相宜本草化妆品有限公司 | Combination of traditional Chinese medicine extractive with function of anti-skin aging, preparation and use |
| CN101810556B (en) * | 2010-04-13 | 2013-01-23 | 上海相宜本草化妆品股份有限公司 | Combination of traditional Chinese medicine extractive with function of anti-skin aging, preparation and use |
| CN103566137A (en) * | 2012-11-22 | 2014-02-12 | 冯兆章 | Chinese herbal medicine formula for removing acne |
| CN103432244A (en) * | 2013-09-11 | 2013-12-11 | 余红波 | Composition having acne removing function |
| CN103432244B (en) * | 2013-09-11 | 2016-01-20 | 余红波 | A kind of compositions with acne-removal function |
| CN109045139A (en) * | 2018-11-01 | 2018-12-21 | 苏州卫生职业技术学院 | A kind of Chinese medicine composition and preparation method thereof inhibiting propionibacterium acnes |
| CN110237185A (en) * | 2019-07-04 | 2019-09-17 | 李菊莲 | A kind of face mask of traditional Chinese medicine for treating acne |
| CN110237185B (en) * | 2019-07-04 | 2021-12-28 | 李菊莲 | Traditional Chinese medicine mask for treating acne |
| CN111494598A (en) * | 2020-06-02 | 2020-08-07 | 郭宝云 | Oral and external traditional Chinese medicine for treating liver-stagnation and spleen-deficiency type acne and preparation method thereof |
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