CN101322725B - Preparation of plant parhormone for treating hypertrophy of the prostate - Google Patents
Preparation of plant parhormone for treating hypertrophy of the prostate Download PDFInfo
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- CN101322725B CN101322725B CN 200810022253 CN200810022253A CN101322725B CN 101322725 B CN101322725 B CN 101322725B CN 200810022253 CN200810022253 CN 200810022253 CN 200810022253 A CN200810022253 A CN 200810022253A CN 101322725 B CN101322725 B CN 101322725B
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Abstract
The invention discloses a plant parahormone for treating prostatic hypertrophy and a preparation method and use thereof, and relates to the technical field of process for extracting active substances from plants, in particular from pollen. The method comprises the following steps: the pollen is frozen rapidly at the ambient temperature of minus 15- minus 30 DEG C, the frozen pollen is soaked by the boiled water at the normal pressure, the solid impurities are removed, the parahormone solution is taken, and finally, the parahormone with the molecular weight of 460-480 is separated at the ambient temperature of 1-5 DEG C, and the parahormone is the plant parahormone for treating prostatic hypertrophy. The plant parahormone of the invention has remarkable effect for treating prostatic hypertrophy, no side effect, and low cost, and the preparation method thereof is simple and feasible.
Description
Technical field
The present invention relates to from plant, particularly from pollen, extract the Technology field of active substance.
Background technology
Prostate gland is an attached body of gland maximum in the male genetic urinary system, is positioned at the bladder below, and just as chestnut, its excretory prostatic fluid is the important component of seminal fluid.Prostatosis are exactly one of common disease that threatens middle-aging male health since ancient times.In recent years, because the quickening of rhythm of life, the change of food habits and living habit caused the increasing of prostate gland morbidity ratio and the rejuvenation of age of onset, so prostatosis is called " cancer that is not cancer " by medical circle.The prostate gland patient often shows urgent urination, frequent micturition, urine wait etc., also can cause prostate cancer and uremic generation when serious.
Prostatosis comprise prostatitis, hyperplasia of prostate, tuberculosis of prostate, calculus of prostate, abscess of prostate, prostate cancer etc.Hyperplasia of prostate wherein, prostatitis is comparatively common, because the people is behind person in middle and old age, the ratio of the intravital sex hormone secretion of the male sex is overbalance gradually, cause testosterone to change into dihydrotestosterone too much, thereby cause hyperplasia of prostate enlargement (being also referred to as prostatomegaly), prostatomegaly is the common disease of middle-aging male, and often with prostatitis.According to statistics, the male sex more than 50 years old has the prostatomegaly of various degrees over half, and along with the increase sickness rate at age also can increase, the male sex after climacterium suffers from the ratio of prostatomegaly up to 75%
[1]Shanghai first medical college was also once investigated more than 20 ten thousand inpatient, find by analysis, person in middle and old age in the disease of easy trouble prostatomegaly make number one, its patient accounts for by 98.66% of investigation patient number
[2]Urethra can be oppressed or stimulate to loose prostate gland, thereby cause urgent urination, frequent micturition, misnicturition etc., male body Health and Living quality had a significant impact, and can cause multiple complications.
Several different methods treatment prostatomegaly is arranged at present, mainly contain these two kinds of methods of operative treatment and pharmacological agent.Operative treatment mainly is the outgrowth prostate gland of excision, but it is dangerous high, and directly influences patient's body Health and Living quality, generally has only severe patient's employing in late period.The medicine of treatment prostatomegaly comprises: chemicals preparation and natural galenical.The chemicals preparation mainly comprises α
1-receptor-blocking agent, inhibitor for androgen, aromatase inhibitor, growth factor receptor inhibitors etc.Though the chemicals preparation can reach better therapeutic effect, because the permeability of capsula prostatica is poor, and a little less than the blood circulation, be difficult to reach therapeutic purpose, and many chemical classes preparations only are the symptoms of releiving, and take stopgap measures and do not effect a permanent cure, and generally all have certain side effect.
Summary of the invention
The present invention's first purpose is to invent a kind of plant parhormone that is used for the treatment of prostatomegaly of fundamentally efficiently treating prostatomegaly.
Parahormone of the present invention is 460～480 parahormone for the molecular weight that extracts from pollen.
Second preparation method that purpose is to invent above-mentioned parahormone of the present invention.
May further comprise the steps:
1) with pollen quick-frozen under-15～-30 ℃ envrionment temperature;
2) with the pollen after the normal pressure boiling water immersion quick-frozen;
3) remove solid impurity, get parahormone solution;
4) isolating molecular weight under 1～5 ℃ envrionment temperature is 460～480 parahormone, is the plant parhormone that is used for the treatment of prostatomegaly.
Through test, utilize pollen treatment prostatomegaly effect remarkable, and have no side effect, and expense is cheap, simple.Pollen is the interior fine granularity thing of sporangiole on the little spore leaf of angiosperm stamen flower pesticide and spore plants, is the male sex-cell of plant.Pollen includes various rich nutrient substances, has multiple biologic activity.Pollen can be brought into play the pharmacological action of class oestrogenic hormon, androgen antagonist on the receptor level of hormone, the biological effect that suppresses dihydrotestosterone, it is approximate normal that outgrowth body of gland is reversed, and the function that can regulate the bladder urethral smooth muscle, but few in the pollen being the research at present of working of what material.This patent is exactly that the method for utilizing water to carry is isolated the composition that prostatomegaly is had result of treatment from Flos Camelliae Japonicae powder.
The quick-frozen time of step 1) of the present invention is 24～48 hours.Purpose is that water molecules in the pollen is freezed, and is convenient to the extraction of effective ingredient in the pollen and keeps active.
Step 2 of the present invention) soak time is 10～15 minutes in.Purpose is to be convenient in the pollen effective ingredient fully dissolve, separate.
The method of removing solid impurity in the step 3) of the present invention is to be in 4000～6000 whizzers that change centrifugal 10～15 minutes at rotating speed.Purpose is a solid impurity in the pollen of effective place to go, makes the extracting solution clarification, and is transparent.
Step 4) of the present invention is described to be to be in 4000～6000 whizzers that change centrifugal 10～15 minutes at rotating speed from going out.Purpose is a water-soluble foreign protein in the pollen extracting liquid of effective place to go, and extracting solution is further purified.
Still a further object of the present invention is to invent the application method of above-mentioned parahormone.
A kind of application method is: the plant parhormone that is used for the treatment of prostatomegaly is used to prepare the oral preparations for the treatment of prostatomegaly.
Advantage as oral preparations is to use convenience, good absorbing effect, cheap, can be used as male sex's use that generally keeps healthy, the generation of prevention prostatomegaly.
Second kind of application method is: the plant parhormone that is used for the treatment of prostatomegaly is used to prepare the injection formulations for the treatment of prostatomegaly.
Advantage as injection formulations is that absorption is rapid, treatment is with strong points.
Description of drawings
Fig. 1 is a natural health group prostata tissue slice map.
Fig. 2 is the partial enlarged drawing of Fig. 1.
Fig. 3 is injection Uniteston control group prostata tissue slice map.
Fig. 4 is the partial enlarged drawing of Fig. 3.
Fig. 5 is an injection injection preparation B group prostata tissue slice map.
Fig. 6 is the partial enlarged drawing of Fig. 5.
Fig. 7 is the full pollen group prostata tissue slice map of feeding.
Fig. 8 is the partial enlarged drawing of Fig. 7.
Fig. 9 is the oral preparations A group prostata tissue slice map of feeding.
Figure 10 is the partial enlarged drawing of Fig. 9.
Embodiment
One, preparation plant parhormone:
1, go-on-go pollen (can be Flos Camelliae Japonicae powder or Pollen Brassicae campestris or Pollen Pini or Flos Nelumbinis pollen or pollen of Semen Fagopyri Esculenti or seed melon pollen): remove pollen impurity at normal temperatures.
2, with the quick-frozen 24～48 hours under-15～-30 ℃ envrionment temperature of the pollen of removal of impurities.
3, soak pollen 10～15 minutes after the quick-frozen with the normal pressure boiling water.
4, remove solid impurity, get parahormone solution:
At rotating speed is in 4000～6000 whizzers that change centrifugal 10～15 minutes, gets supernatant.
5, be in 4000～6000 whizzers that change centrifugal 10～15 minutes under 1～5 ℃ the envrionment temperature, at rotating speed, isolating molecular weight and be 460～480 parahormone, be the plant parhormone that is used for the treatment of prostatomegaly.
Two, preparation oral preparations and injection formulations:
1, preparation oral preparations A:
Plant parhormone and honey or other foodstuff additive are mixed well, and after ultrafiltration and pasteurization sterilization, can is standby.
2, preparation injection formulations B:
Plant parhormone pasteurization sterilization back and injection water is mixed with 1: 1～2 volume ratio, vacuumize sealing after the uniform dissolution, stand-by.
Three, use:
(1) subjects:
Rat (body weight 500～600 grams)
(2) using dosage:
1, oral preparations: oral 5-10 milliliter/sky connects and took 30～60 days.
2, injection: muscle or abdominal injection 1-2 milliliter/sky 20-30 days continuously
(3) comparing result:
Test group SD in age in April rat prostate tissue slice result compares:
1, natural health group prostata tissue slice map is seen Fig. 1,2.
From Fig. 1, the existing loose symptom of 2 visible 4 monthly age male white rat prostate glands, there is the wall that wrinkles in the part prostate gland acinus to increase.
2, injection Uniteston control group prostata tissue slice map is seen Fig. 3,4.
Obvious from Fig. 3,4 visible prostatomegaly symptoms, the wrinkle wall is a lot of in the prostate gland acinus.
3, injection formulations B group prostata tissue slice map is seen Fig. 5,6.
Not obvious from Fig. 5,6 visible prostatomegaly symptoms, the wrinkle wall seldom in the prostate gland acinus.
4, the full pollen group prostata tissue slice map of feeding is seen Fig. 7,8.
Not obvious from Fig. 7,8 visible prostatomegaly symptoms, the wrinkle wall is less in the prostate gland acinus.
5, the oral preparations A group prostata tissue slice map of feeding is seen Fig. 9,10.
More obvious from Fig. 9,10 visible prostatomegaly symptoms, the wrinkle wall is more in the prostate gland acinus.
The normal adult rat prostate is the branch tubular gland, and prostate gland acinus epithelium mostly is simple cuboidal epithelium or pseudostratified columnar epithe lium, and the glandular epithelium secretory cell stretches into to alveolar lumen and forms many wrinkle walls.When the generation prostatomegaly, because a large amount of prostatic epithelium secretory cells propagation and increase, the wall that causes wrinkling in the alveolar lumen increases, and prostate gland increases, weightening finish.Cut into slices from prostata tissue, it is less that the prostate gland acinus epithelium secretory cell of injecting the test group of full pollen with the pollen aqueous extract forms the wall that wrinkles, and the acinus inwall is more smooth.
Result of treatment with the test group of injection formulations B injection is better than full pollen and oral preparations A, thereby can affirm that oral preparations A of the present invention and injection formulations B can will put forward the medicative composition of prostatomegaly in the pollen really.
Serum testosterone, estradiol and prostate specific antigen detected result cartogram (unit: ng/ml)
Check each test group serum testosterone difference not remarkable through ANOVA.
Each test group serum estradiol content is fed, and oral preparations A organizes and the natural control group and the full pollen group of feeding, and injection Uniteston group difference is not remarkable; Injection injection preparation B group and feed full pollen and natural control group significant difference.
Each test group serum prostate specific antigen content is fed, and oral preparations A organizes and injects the Uniteston group and injection injection preparation B group difference is not remarkable; The injection Uniteston group and the full pollen significant difference of feeding.
Each test group serum estradiol content difference is remarkable, the oral preparations A that feeds group and the natural control group and the full pollen group of feeding, and injection Uniteston group difference is not remarkable; Injection injection preparation B group and feed full pollen and natural control group significant difference.This explanation contains the composition of regulation and control serum estradiol content in injection formulations B.
The serum prostate specific antigen is the index of reflection prostatitis and injury of prostate degree, feeding from this test, to organize difference not remarkable for oral preparations A group serum prostate specific antigen and injection injection preparation B, the oral preparations A that feeds organizes not as injection injection preparation B the effect of the reparation of prostatitis and injury of prostate with the full pollen of feeding, and this explanation contains the material that promotes the injury of prostate reparation in injection formulations B.
In a word, show by above test: product of the present invention can effectively improve the treatment prostatomegaly, and the curative effect of injection formulations is better than oral preparations.
Claims (1)
1. preparation method who is used for the treatment of the plant parhormone of prostatomegaly is characterized in that may further comprise the steps:
1) with pollen quick-frozen 24～48 hours under-15～-30 ℃ envrionment temperature;
2) soak pollen 10～15 minutes after the quick-frozen with the normal pressure boiling water;
3) remove solid impurity, get parahormone solution; Described method of removing solid impurity is to be in 4000～6000 whizzers that change centrifugal 10～15 minutes at rotating speed;
4) be in 4000～6000 whizzers that change centrifugal 10～15 minutes under 1～5 ℃ the envrionment temperature, at rotating speed, isolating molecular weight and be 460～480 parahormone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200810022253 CN101322725B (en) | 2008-06-30 | 2008-06-30 | Preparation of plant parhormone for treating hypertrophy of the prostate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200810022253 CN101322725B (en) | 2008-06-30 | 2008-06-30 | Preparation of plant parhormone for treating hypertrophy of the prostate |
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| Publication Number | Publication Date |
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| CN101322725A CN101322725A (en) | 2008-12-17 |
| CN101322725B true CN101322725B (en) | 2010-12-01 |
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2008
- 2008-06-30 CN CN 200810022253 patent/CN101322725B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 冯晓慧等.蜂产品激素知多少.家庭医学（新健康）.2007,(12),44-45. * |
| 陈其庚等.油菜素内酯(第六大类激素)的功用.福建果树.1996,(2),27-29. * |
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Owner name: YANTAI NEW ERA HEALTH INDUSTRY CO., LTD. Free format text: FORMER OWNER: ZHOU BIN Effective date: 20100825 |
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Free format text: CORRECT: ADDRESS; FROM: 225009 COLLEGE OF ANIMAL SCIENCE AND TECHNOLOGY, YANGZHOU UNIVERSITY, YANGZHOU CITY, JIANGSU PROVINCE TO: 264006 NO.6, HUAIHE ROAD, YANTAI DEVELOPMENT ZONE, SHANDONG PROVINCE |
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Effective date of registration: 20100825 Address after: 264006 No. 6, Huaihe Road, Yantai Development Zone, Shandong Applicant after: Yantai new era Health Industry Co., Ltd. Address before: 225009 College of animal science and technology, Yangzhou University, Yangzhou, Jiangsu Applicant before: Zhou Bin |
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Inventor after: Liu Zhihe Inventor after: Zhou Bin Inventor after: Shi Lihua Inventor after: Wang Tong Inventor before: Zhou Bin |