CN101152214A - External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same - Google Patents
External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same Download PDFInfo
- Publication number
- CN101152214A CN101152214A CNA2007101131166A CN200710113116A CN101152214A CN 101152214 A CN101152214 A CN 101152214A CN A2007101131166 A CNA2007101131166 A CN A2007101131166A CN 200710113116 A CN200710113116 A CN 200710113116A CN 101152214 A CN101152214 A CN 101152214A
- Authority
- CN
- China
- Prior art keywords
- rhizoma dioscoreae
- sperm
- nutrient solution
- polysaccharide
- hour
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a yam in vitro sperm nutrient solution, the preparation method and the application of the solution. The solution is mixed by polysaccharide extracted from yam raw material and normal saline, wherein the ratio between the yam polysaccharide and the normal saline is that 1 gram to 10 millimeter. The preparation method is: yam raw material is cleaned, dried and smashed, and is drenched in normal saline; then the soak is put in a vessel, heated under conditions between 95 to 105 degrees centigrade, and filtered to produce yam extract. The application of the yam in vitro sperm nutrient solution is: the yam in vitro sperm nutrient solution is mixed uniformly with semen with a liquid volume ratio of 1 to 1 to cultivate sperm and activated sperm. The invention is capable of enhancing the sperm mobility by 35 to 56 percent without any damage to or side effects on sperm, so the product with such high effects can replace imported product. Besides, the price is greatly reduced compared with imported goods.
Description
Technical field
The present invention relates to sperm nutrient solution, is a kind of Rhizoma Dioscoreae external sperm nutrient solution and preparation method thereof and application.
Background technology
Because low sperm activity and infertile patients with infertility accounts for the large percentage of patients with infertility, it is a lot of to treat this class infertility oral drugs kind, but, no matter Western medicine or Chinese medicine, its curative effect is all not good enough, even some patients needed just can take effect in oral several years, causes bigger financial burden and mental pressure to the patient, and be prone to various senses of discomfort, patient body health there is certain influence.For this reason, the use sperm nutrient solution that began one's study is in the world in recent years cultivated at external vigor to patient's sperm, finally reaches the purpose that strengthens motility of sperm.This nutritional solution of China's use at present is imported product, costs an arm and a leg, and generally all contains the chemicals composition in these nutritional solutions.Yet the ultimate aim of this area research is to use pure natural substance.
Summary of the invention
The objective of the invention is, a kind of Rhizoma Dioscoreae external sperm nutrient solution and preparation method thereof and application are provided, it adopts the pure natural substance Rhizoma Dioscoreae is that raw material extracts wherein polysaccharide, makes sperm nutrient solution, makes sperm strengthen motility of sperm in external activation to reach.
The present invention for achieving the above object, be achieved through the following technical solutions: a kind of Rhizoma Dioscoreae external sperm nutrient solution, it is polysaccharide and the blended liquid of normal saline that extracts in the Rhizoma Dioscoreae raw material, and the ratio of Rhizoma Dioscoreae polysaccharide and normal saline is 1 gram Rhizoma Dioscoreae polysaccharide: 10 ml physiological salines.
The preparation method of described a kind of Rhizoma Dioscoreae external sperm nutrient solution, getting the Rhizoma Dioscoreae raw material cleans it, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Rhizoma Dioscoreae raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains the Rhizoma Dioscoreae extracting solution;
The Rhizoma Dioscoreae extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% live carbon decoloring 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain the Rhizoma Dioscoreae crude polysaccharides;
Again the Rhizoma Dioscoreae crude polysaccharides is carried out separation and purification: obtain a Rhizoma Dioscoreae polysaccharide component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care the Rhizoma Dioscoreae polysaccharide after the vacuum lyophilization;
Make with extra care the ratio that the Rhizoma Dioscoreae polysaccharide adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made Rhizoma Dioscoreae polysaccharide sperm nutrient solution.
Described soak was at vessel in heating 10-45 minute.
The ratio of described Rhizoma Dioscoreae raw material and normal saline is 100g: 1000 milliliters.
The application of described a kind of Rhizoma Dioscoreae external sperm nutrient solution, Rhizoma Dioscoreae sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
Rhizoma Dioscoreae raw material of the present invention is a Chinese medicine simply, and its property is sweet, warm, flat, nontoxic, is Dioscoreaceae plant Rhizoma Dioscoreae.Cold and heat pathogen, YANG invigorating are won, removed to the Rhizoma Dioscoreae tonify deficiency, and invigorating the spleen and replenishing QI has the merit of transferring benefit.Because of its sweet in the mouth gas perfume (or spice), help spleen etc. with it.Medical research shows: Rhizoma Dioscoreae contains mannan, and allantoin, dopamine, phytic acid and 16 seed amino acids etc. can be treated trusted subordinate's deficiency-distension, deadly cold hand and foot, expectorant wind dyspnea with rapid respiration, weakness of the spleen and stomach, damp and hot diarrhea due to deficiency, toxic swelling from the beginning of etc.
Modern Chinese medicine is extensively treated cancer with Rhizoma Dioscoreae, cardiovascular and cerebrovascular disease, respiratory tract disease etc.
Further studies show that: the polysaccharide in the Rhizoma Dioscoreae has bigger effect to the activation of external sperm.
The present invention adopts the raw material of Rhizoma Dioscoreae as external sperm nutrient solution, the sperm nutrient solution of producing is through experimental results show that, can improve sperm motility 35-56%, prolong 24-28 hour sperm in vitro time-to-live, sperm there is not any infringement, without any side effects, the complete import substitutes of its effect, and price reduces significantly than imported product, for China's patients with infertility provides a kind of economical and practical, safety and the outstanding product of obvious results.
The specific embodiment
Embodiment:
Rhizoma Dioscoreae external sperm nutrient solution of the present invention is to adopt polysaccharide and the blended liquid of normal saline that extracts in the Rhizoma Dioscoreae raw material, and the ratio of Rhizoma Dioscoreae polysaccharide and normal saline is 1 gram Rhizoma Dioscoreae polysaccharide: 10 ml physiological salines.
The preparation method of described Rhizoma Dioscoreae external sperm nutrient solution is, getting Rhizoma Dioscoreae is that raw material is cleaned it, drying is 2 hours under 60 ℃, pulverize then, insert in 0.8% the normal saline and soaked 1 hour, the ratio of Rhizoma Dioscoreae raw material and normal saline is 100g: the 900-1150 milliliter, this ratio can be: 100g: 900 milliliters, 100g: 1150 milliliters or 100g: 1100 milliliters etc. all can, preferred scheme is: the ratio of raw material Rhizoma Dioscoreae and normal saline is 100g: 1000 milliliters.Then soak is inserted in the container and heat down at 100 ℃, the taking-up after-filtration removes slag and obtains the Rhizoma Dioscoreae extracting solution; The Rhizoma Dioscoreae extracting solution was descended dry 1 hour at 60 ℃, obtain dry thing; According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims, again filtering residue is volatilized behind the solvent with dipping in 250 milliliters of 80% ethanol 10 hours or 12 hours or 15 hours all can, 80 ℃ of-90 ℃ of following reflux, extract, 2 times, each 1.2 hours, obtain filtrate and filtering residue; Again with filtering residue adding distil water 450-550 milliliter, preferred 500 milliliters are soaked after 0.8-1.2 hour 90 ℃ of following warm macerating 40 minutes, filter, and obtain filtrate and filtering residue, again with filtering residue with 250 ml distilled waters 90 ℃ of following warm macerating 30 minutes, filtration obtains filtrate; Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters; Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 60 ℃ of down oven dry, obtain the Rhizoma Dioscoreae crude polysaccharides; Again the Rhizoma Dioscoreae crude polysaccharides is carried out separation and purification: obtain a Rhizoma Dioscoreae polysaccharide component with distilled water eluting cellulose column; Reuse 0-0.-2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care the Rhizoma Dioscoreae polysaccharide after the vacuum lyophilization; Make with extra care the ratio that the Rhizoma Dioscoreae polysaccharide adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made Rhizoma Dioscoreae polysaccharide sperm nutrient solution.
Soak of the present invention is generally in the time of vessel in heating and was advisable in 10-45 minute.
The application of a kind of Rhizoma Dioscoreae external sperm nutrient solution of the present invention is, Rhizoma Dioscoreae sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, is used to cultivate sperm and activation sperm, to reach effect of the present invention.
Claims (5)
1. Rhizoma Dioscoreae external sperm nutrient solution, it is characterized in that: it is polysaccharide and the blended liquid of normal saline that extracts in the Rhizoma Dioscoreae raw material, the ratio of Rhizoma Dioscoreae polysaccharide and normal saline is 1 gram Rhizoma Dioscoreae polysaccharide: 10 ml physiological salines.
2. the preparation method of the described a kind of Rhizoma Dioscoreae external sperm nutrient solution of claim 1, it is characterized in that: get the Rhizoma Dioscoreae raw material it is cleaned, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Rhizoma Dioscoreae raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains the Rhizoma Dioscoreae extracting solution;
The Rhizoma Dioscoreae extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain the Rhizoma Dioscoreae crude polysaccharides;
Again the Rhizoma Dioscoreae crude polysaccharides is carried out separation and purification: obtain a Rhizoma Dioscoreae polysaccharide component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care the Rhizoma Dioscoreae polysaccharide after the vacuum lyophilization;
Make with extra care the ratio that the Rhizoma Dioscoreae polysaccharide adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made Rhizoma Dioscoreae polysaccharide sperm nutrient solution.
3. the preparation method of a kind of Rhizoma Dioscoreae external sperm nutrient solution according to claim 2 is characterized in that: soak was at vessel in heating 10-45 minute.
4. the preparation method of a kind of Rhizoma Dioscoreae external sperm nutrient solution according to claim 2 is characterized in that: the ratio of Rhizoma Dioscoreae raw material and normal saline is 100g: 1000 milliliters.
5. the application of each described a kind of Rhizoma Dioscoreae external sperm nutrient solution of claim 1-4 is characterized in that: Rhizoma Dioscoreae sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101131166A CN101152214A (en) | 2007-09-27 | 2007-09-27 | External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101131166A CN101152214A (en) | 2007-09-27 | 2007-09-27 | External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101152214A true CN101152214A (en) | 2008-04-02 |
Family
ID=39254233
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101131166A Pending CN101152214A (en) | 2007-09-27 | 2007-09-27 | External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101152214A (en) |
-
2007
- 2007-09-27 CN CNA2007101131166A patent/CN101152214A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102018810B (en) | Preparation method of compound Houttuynia cordata mixture | |
| CN103750107B (en) | Health-care product with blood glucose reducing function | |
| CN104351563A (en) | Functional feed used for preventing pig malignant edema and preparation method of functional feed | |
| CN104306679A (en) | Chinese herb agentia for treating epidemic cerebrospinal meningitis and preparation method thereof | |
| CN101279051A (en) | Preparation of Chinese medicine for curing phlegm-heat stasis type bronchitis | |
| CN101152213A (en) | External spermatozoon nutrient solution of ganoderma lucidum, method for preparing the same and application of the same | |
| CN109172671A (en) | A kind of flue moxibustion traditional Chinese medicine powder and preparation method thereof for kidney yang deficiency syndrome patient | |
| CN101152214A (en) | External spermatozoon nutrient solution of dioscorea opposite, method for preparing the same and application of the same | |
| CN101152212A (en) | Tuckahoe external spermatozoon nutrient solution, method for preparing the same and application of the same | |
| CN101139572B (en) | Ophiopogon root external sperm nutrient solution and preparation method and application thereof | |
| CN105853901A (en) | Preparation for cancer patients to take during radiotherapy and chemotherapy, and method for preparing preparation | |
| CN105169102A (en) | Lung-clearing decoction | |
| CN104771524A (en) | Traditional Chinese medicine composition for treating neurasthenia and preparation method of traditional Chinese medicine composition | |
| CN1319546C (en) | Delayed-releasing preparation for treating osteoporosis | |
| CN107929415A (en) | A kind of Chinese medicine composition for treating the infection of the upper respiratory tract and preparation method thereof | |
| CN104095928B (en) | Medicine for treating proteus mirabilis type digestive tract disease | |
| CN103599259A (en) | Dai medicine extract with hypoglycemic effect, preparation and application thereof | |
| CN108835493B (en) | Preparation method of health-care beverage | |
| CN105616516A (en) | Epidemic cerebrospinal meningitis treating injection and preparation method thereof | |
| CN105148199A (en) | Chinese-Western compound pharmaceutical preparation for treating rheumatoid arthritis | |
| CN1951472A (en) | Yin-nourishing body liquid-generating Chinese medicine and preparation method thereof | |
| CN105998671A (en) | Traditional Chinese medicine composition for treating cough and preparation method thereof | |
| CN115364167A (en) | Traditional Chinese medicine oral liquid for treating cow mastitis and preparation method thereof | |
| CN103230511A (en) | Traditional Chinese medicine for treating swollen sore throat and cough and preparation method thereof | |
| CN104162095A (en) | Paste capable of clearing abdominal dropsy and relieving pain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20080402 |