CN103599259A - Dai medicine extract with hypoglycemic effect, preparation and application thereof - Google Patents
Dai medicine extract with hypoglycemic effect, preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a Dai medicine extract with a hypoglycemic effect, preparation and application thereof. The Dai medicine extract is extracted from the Dai medicines Dischidia minor (Vahl.) Merr., Aspidopterys obcordata Hemsl., Passiflora wilsonii Hemsl., and Clerodendrum japonicum (Thunb.) Sweet. The invention also discloses a preparation method of the extract, and also discloses application of the extract and its medicinal composition as medicines and health care products, especially application in preparation of hypoglycemic medicines and health care products.
Description
Technical field
The present invention relates to a kind of from Dai Nationality's drug anesthesia baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through (Aspidopterys obcordata Hemsl.), bud Nan Ba (Passiflora wilsonii Hemsl.), the extract with remarkable hypoglycemic activity extracting in guest bright (Clerodendrum japonicum (Thunb.) Sweet), and the preparation method of this extract, the drug regimen that contains this extract, and this extract and the application of its drug regimen as medicine and health product, be particularly useful for the preparation of hypoglycemic drug and health product, belong to medicine, field of health care products.
Background technology
Diabetes are a kind of whole body chronic progressive diseases.Due to the normal excreting insulin of islet cells, thereby insulin relatively or definitely not enough, and the reduction of target cell to insulin sensitivity, and cause sugar, albumen, fat and water, metabolic disturbance of electrolyte, and hepatic glycogen and muscle glycogen can not be synthesized.Clinical manifestation is blood sugar increasing, the glucose in urine positive, impaired glucose tolerance etc., long-term ill meeting is in succession with a series of complication, and because people's living standard raises, living habit changes, China's diabetics number constantly increases, and becomes and is only second to India second largest country in the world.
For the medicine of diabetes, taboo and untoward reaction are more at present, and long-term taking, easily causes liver toxicity and digestive tract side effects problem.Therefore from traditional medicine, find active strong, the low active drug of side effect and become the new way addressing this problem.Dai Nationality's drug anesthesia baa cloth (Dischidia minor (Vahl.) Merr.) is the leaf of asclepiadaceae plant Dischidia minor (Vahl) Merr, sour in the mouth, cool in nature.Enter water tower, in Dai Nationality's medicine traditional classical, record its Qinghuo Jiedu, reducing swelling and alleviating pain, grow hydrolysis yearningly, cure mainly high hot dry throat and mouth, the diseases such as tired thist with desire to drink, also can treat the symptoms such as parotitis, submandibular lymph nodes, relieving mammary gland gall; He lid pass through the rattan that (Aspidopterys obcordata Hemsl.) is the Malpighiaceae plant Caulis Aspidopterydis obcordatae of falling the heart, lightly seasoned, micro-hardship, cold in nature, enter water tower, function diuretic calculus, heat-clearing and toxic substances removing, tradition is applied to edema disease; Bud Nan Ba (Passiflora wilsonii Hemsl.) is the herb of Passifloraceae plant Passiflora wilsonii, bitter in the mouth, and property is flat, inlet air, fire, water tower, function compensate qi and blood, removing damp and stopping pain, building body, the disease such as tradition is applied to soreness of the waist and knees, sexual impotence; Guest bright (Clerodendrum japonicum (Thunb.) Sweet) remits root and the leaf of paulownia for Verbenaceae, taste is sweet, and property is flat, enters water, Feng Ta, effect heat-clearing and toxic substances removing, cooling blood for hemostasis, lactogenesis stimulating milk secretion, menstruction regulating and pain relieving, propose gas-solid de-, strengthening the tendons and bones, the disease such as tradition is applied to limbs fatigue, soreness of the waist and knees.The main inlet air of Si Zhong Dai Nationality medicine, water tower, after mixing by a certain percentage, can strengthen its cooling blood for hemostasis, grow the thirsty effect of hydrolysis, can be used for treating the diseases such as tired thist with desire to drink, we find by Pharmacodynamics screening, by extracting the extract obtaining after compatibility, can obviously reduce hyperglycemic rat blood glucose value, effect be use now medicine metformin 2-5 doubly, there is consumption low, the effect of successful.
Summary of the invention
The object of the present invention is to provide from Dai Nationality's drug anesthesia baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through (Aspidopterys obcordata Hemsl.), bud Nan Ba (Passiflora wilsonii Hemsl.), guest bright (Clerodendrum japonicum (Thunb.) Sweet) a kind of extract with hypoglycemic activity of extraction.
Another object of the present invention is to provide the method for this extract of preparation.
Another object of the present invention is to propose this extract and has the pharmaceutical preparation of hypoglycemic activity and the application in health food in preparation.
In order to achieve the above object, the present invention has taked following technical scheme:
(1) get numb baa cloth (Dischidia minor (Vahl.) Merr.) 20-50 part, he lid pass through (Aspidopterys obcordata Hemsl.) 10-30 part, bud Nan Ba (Passiflora wilsonii Hemsl.) 20-40 part, guest bright (Clerodendrum japonicum (Thunb.) Sweet) 10-30 part, after normal temperature drying, co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add quality of medicinal material 10-30 70% ethanol doubly, heating and refluxing extraction, extraction time is 1-3 time.
(3) get in (2) and extract the medicinal liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains the extractum that relative density is 1.15-1.5.
(4) get the extractum obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, be splined in silicagel column, applied sample amount is calculated as 20-40 doubly with extractum amount and the ratio of silica gel amount, static adsorption 30 minutes, then with 50% ethanol of the water of 10 column volumes, 20% ethanol of 10 column volumes and 10 column volumes, carry out eluting successively, collect 50% ethanol elution.
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains the extractum that relative density is 1.15-1.5.
(6) get the extractum obtaining in (5), carry out drying under reduced pressure in vacuum drying oven, heating-up temperature is no more than 60 ℃, crushed after being dried.Obtain extract provided by the invention.
As to the improvement of technique scheme with supplement:
(7), when the medicinal powder obtaining in to (1) extracts, extracting solvent can be 30%-100% ethanol.
(8), when the medicinal powder obtaining in to (1) extracts, extracting mode can be that heating and refluxing extraction, supersound extraction or percolation extract.
(9), when the extractum obtaining carries out silica gel enrichment, use the silica gel into middle polarity in to (3).
The extract preparing by technique scheme is the plant extract of the pure natural of dietotherapeutic, and effect experiment subsequently will prove, extract of the present invention has significant blood sugar lowering damaging action.
The invention still further relates to and using extract of the present invention as active component, add the pharmaceutical composition of conventional medicine excipient or adjuvant.Conventionally the extract of the present invention that pharmaceutical composition of the present invention contains 0.1-95% weight.
The pharmaceutical composition of extract of the present invention can be according to method preparation well known in the art.Except extract of the present invention, in the scope of not overslaugh effect of the present invention, can suitably be mixed for as required in other compositions of common pharmaceutical preparation and health food, such as: in crospolyvinylpyrrolidone, carboxymethylstach sodium, microcrystalline Cellulose, lactose, aspartame, magnesium stearate etc., add adjuvant, additional adjuvant and coating adjuvant, its dosage form can be any pharmaceutically said dosage form, preferred tablet, pill, capsule or oral liquid.
The specific embodiment
Below in conjunction with specific embodiment; further set forth the present invention; these embodiment are interpreted as only for the present invention is described, is not used in and limits the scope of the invention; after having read the content of the present invention's record; those skilled in the art can make various changes or modifications the present invention, and these equivalences change and modification falls into the claims in the present invention book limited range equally.
The preparation of embodiment 1 Dai Nationality's medicament extract
(1) get 20 parts of numb baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through 10 parts of (Aspidopterys obcordata Hemsl.) 10 parts, 20 parts of bud Nan Ba (Passiflora wilsonii Hemsl.), guests bright (Clerodendrum japonicum (Thunb.) Sweet), co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 30% ethanol of 10 times of quality of medicinal materials, heating and refluxing extraction, extraction time is 1 time.
(3) get in (2) and extract the medicinal liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.15 extractum.
(4) get the extractum obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, be splined in silicagel column, applied sample amount is calculated as 20 times with extractum amount and the ratio of silica gel amount, static adsorption 30 minutes, then use successively the water of 10 column volumes, 20% ethanol of 10 column volumes, collect 20% ethanol elution
(5) get eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.15 extractum.
(6) get the extractum obtaining in (5), carry out drying under reduced pressure in vacuum drying oven, heating-up temperature is no more than 60 ℃, crushed after being dried.Obtain extract provided by the invention.
The preparation of embodiment 2 Dai Nationality's medicament extracts
(1) get 50 parts of numb baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through 30 parts of (Aspidopterys obcordata Hemsl.) 30 parts, 40 parts of bud Nan Ba (Passiflora wilsonii Hemsl.), guests bright (Clerodendrum japonicum (Thunb.) Sweet), co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 100% ethanol of 10 times of quality of medicinal materials, supersound extraction, extraction time is 3 times.
(3) get in (2) and extract the medicinal liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.5 extractum.
(4) get the extractum obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, be splined in silicagel column, applied sample amount is calculated as 40 times with extractum amount and the ratio of silica gel amount, static adsorption 30 minutes, then successively with the water of 10 column volumes, 20% ethanol of 10% ethanol of 10 column volumes, 10 column volumes,, 50% ethanol of 30% ethanol of 10 column volumes and 10 column volumes carries out eluting, is collected 50% ethanol elution
(5) get eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.5 extractum.
(6) get the extractum obtaining in (5), carry out drying under reduced pressure in vacuum drying oven, heating-up temperature is no more than 60 ℃, crushed after being dried.Obtain extract provided by the invention.
The preparation of embodiment 3 Dai Nationality's medicament extracts
(1) get 30 parts of numb baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through 20 parts of (Aspidopterys obcordata Hemsl.) 20 parts, 30 parts of bud Nan Ba (Passiflora wilsonii Hemsl.), guests bright (Clerodendrum japonicum (Thunb.) Sweet), co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 70% ethanol of 10 times of quality of medicinal materials, supersound extraction, extraction time is 2 times.
(3) get in (2) and extract the medicinal liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.28 extractum.
(4) get the extractum obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, be splined in silicagel column, applied sample amount is calculated as 30 times with extractum amount and the ratio of silica gel amount, static adsorption 30 minutes, then successively with the water of 10 column volumes, 20% ethanol of 10% ethanol of 10 column volumes, 10 column volumes,, 40% ethanol of 30% ethanol of 10 column volumes and 10 column volumes carries out eluting, is collected 40% ethanol elution
(5) get eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 ℃, finally obtains relative density and be 1.2 extractum.
(6) get the extractum obtaining in (5), carry out drying under reduced pressure in vacuum drying oven, heating-up temperature is no more than 60 ℃, and crushed after being dried obtains extract provided by the invention.
Case study on implementation 4 (Dai Nationality's medicament extract function of blood sugar reduction research)
1, extract preparation
According to preparation method in embodiment 3, be prepared.
2, animal gavage
Get 40 of white mice, body weight 20 ± 2g, male and female half and half, separately feed, and after 24h, lumbar injection four pyrimidine 200mg/kg, cause Experimental diabetic model, after 48h., are divided into four groups on an empty stomach, and 10 every group, male and female half and half, and carry out gavage, gavage is 5 days continuously.Gavage dosage is as follows:
First group of blank group, gavage normal saline
Second group of hyperglycemia model group, gavage normal saline
The 3rd group of positive controls, gavage metformin 250mg/kgd
Si Zu Dai Nationality medicament extract low concentration group, gavage 100mg/kgd
Wu Zu Dai Nationality medicament extract high concentration group, gavage 250mg/kgd
3, get blood
18h. after the 5th gavage, plucks eyeball by each six (every group each 1) and gets blood (0.3mL/ only), and in every test tube, add in advance anticoagulant trisodium citrate 0.1g.After shaking up, add 6mol/L sulphuric acid 5mL and 10% sodium tungstate 0.7mL, shake up and process 10min, after with the centrifugal 15min of 3500r/min, obtain supernatant and be serum.
4, measuring blood content
Adopt ortho-aminotoluene method to measure (Xu Shuyun, 1991).
5, result
By more known, Dai Nationality's medicament extract high dose group can obviously reduce the white mice blood sugar concentration of the diabetes model being caused by alloxan, and concrete impact the results are shown in Table 1.
Table 1 Dai Nationality medicament extract is to experimental hyperglycemia white mice blood sugar influence result
Claims (10)
1.Yi Zhong Dai Nationality medicament extract, it is characterized in that, described Dai Nationality medicament extract by medical material fiber crops baa cloth (Dischidia minor (Vahl.) Merr.), he lid pass through (Aspidopterys obcordata Hemsl.), bud Nan Ba (Passiflora wilsonii Hemsl.) and guest bright (Clerodendrum japonicum (Thunb.) Sweet) and make.
2. Dai Nationality's medicament extract as claimed in claim 1, it is characterized in that, described medical material takes in weight portion, numb baa cloth (Dischidia minor (Vahl.) Merr.) 20-50 part, he lid pass through (Aspidopterys obcordata Hemsl.) 10-30 part, bud Nan Ba (Passiflora wilsonii Hemsl.) 20-40 part, guest bright (Clerodendrum japonicum (Thunb.) Sweet) 10-30 part.
3. a method of preparing the Dai Nationality's medicament extract described in claim 1-2 any one, comprising: take medical material fiber crops baa cloth (Dischidia minor (Vahl.) Merr.) 20-50 part, he lid pass through (Aspidopterys obcordata Hemsl.) 10-30 part, bud Nan Ba (Passiflora wilsonii Hemsl.) 20-40 part, guest bright (Clerodendrum japonicum (Thunb.) Sweet) 10-30 part; After medical material normal temperature drying, pulverize; After mixing, with extraction after debita spissitudo soak with ethanol, obtain medicinal liquid; Extracting solution concentrates to obtain extractum; Extractum utilizes silicagel column to carry out enrichment; Make powder, be extract of the present invention.
4. method according to claim 3, is characterized in that, described debita spissitudo ethanol is 30%-100% ethanol, and extraction time is 1-3 time, and each consumption is 10 times of amounts, soaks 12 hours.
5. method according to claim 3, is characterized in that, can adopt heating and refluxing extraction, supersound extraction, percolation to extract and obtain medicinal liquid.
6. method according to claim 3, it is characterized in that, described enrichment is to mix and mix thoroughly with isopyknic gel after extractum is added to 5 times of water gaging dilutions, be splined in middle polarity silicagel column, applied sample amount is calculated as 20-40 doubly, static adsorption 30 minutes with extractum amount and the ratio of silica gel amount, then with the ethanol of the variable concentrations of 10 column volumes, carry out eluting successively, collect 20%-50% ethanol elution, in Rotary Evaporators, concentrate, obtain extractum.
7. method according to claim 3, is characterized in that, described powder is that crushed after being dried makes by carry out drying under reduced pressure in vacuum drying oven.
8. method according to claim 3, is characterized in that, when extractum utilizes silicagel column to carry out enrichment, the ethanol that eluting solvent is variable concentrations, according to concentration of alcohol eluting successively from low to high.
One kind as claim 1-2 any one or according to the method for any one in claim 3-8, prepare as described in extract in preparing hypoglycemic pharmaceutical preparation and health food, apply.
10. the pharmaceutical preparation described in claim 9, is characterized in that, this pharmaceutical preparation is tablet, capsule, granule or pill.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104784377A (en) * | 2015-04-24 | 2015-07-22 | 张宁 | Dai medicinal compound extract for preventing and treating Alzheimer disease and application of Dai medicinal compound extract |
| CN108542927A (en) * | 2018-05-25 | 2018-09-18 | 中国医学科学院药用植物研究所云南分所 | The application of the obcordate aspidopterys stem of falling the heart and its extract in anti-tumor aspect |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102178860A (en) * | 2011-04-11 | 2011-09-14 | 赵龙山 | Traditional Chinese medicine liquid for treating diabetes |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104784377A (en) * | 2015-04-24 | 2015-07-22 | 张宁 | Dai medicinal compound extract for preventing and treating Alzheimer disease and application of Dai medicinal compound extract |
| CN104784377B (en) * | 2015-04-24 | 2018-12-25 | 张宁 | A kind of dai medicine compound extract and its application for preventing and treating Alzheimer disease |
| CN108542927A (en) * | 2018-05-25 | 2018-09-18 | 中国医学科学院药用植物研究所云南分所 | The application of the obcordate aspidopterys stem of falling the heart and its extract in anti-tumor aspect |
| CN108542927B (en) * | 2018-05-25 | 2021-12-14 | 中国医学科学院药用植物研究所云南分所 | Application of radix scutellariae sessiliflorae and extract thereof in anti-tumor aspect |
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