CN103599259B - A kind of Dai Nationality's medicament extract preparation and application thereof with hypoglycemic activity - Google Patents
A kind of Dai Nationality's medicament extract preparation and application thereof with hypoglycemic activity Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a kind of Dai Nationality's medicament extract preparation and application thereof with hypoglycemic activity, does is described Dai Nationality medicament extract from the drug anesthesia baa cloth (Dischidia of the Dai Nationality? minor (Vahl.) Merr.), he lid pass through (Aspidopterys? obcordata? Hemsl.), bud Nan Ba (Passiflora? wilsonii? Hemsl.), bright (the Clerodendrum of guest? japonicum (Thunb.) Sweet) middle extraction, the invention also discloses the preparation method of described extract, the invention also discloses the application as medicine and health products of this extract and its pharmaceutical composition, be particularly useful for the preparation of hypoglycemic drug and health products.
Description
Technical field
The present invention relates to a kind of from Dai Nationality's drug anesthesia baa cloth (Dischidiaminor (Vahl.) Merr.), he lid pass through(AspidopterysobcordataHemsl.), bud Nan Ba (PassiflorawilsoniiHemsl.), guest are brightThe extract with remarkable hypoglycemic activity extracting in (Clerodendrumjaponicum (Thunb.) Sweet), andThe preparation method of this extract, the drug regimen that contains this extract, and this extract and its drug regimen are as medicineWith the application of health products, be particularly useful for the preparation of hypoglycemic drug and health products, belong to medicine, field of health care products.
Background technology
Diabetes are a kind of whole body chronic progressive diseases. Due to normally excreting insulin of islet cells, thereby pancreasIsland element is relatively or definitely not enough, and the reduction of target cell to insulin sensitivity, and causes sugar, albumen, fat and water, electrolysisMatter metabolic disorder, can not synthesize hepatic glycogen and muscle glycogen. Clinical manifestation is blood sugar rising, the glucose in urine positive, impaired glucose tolerance etc.,Long-term ill meeting is in succession with a series of complication, and because people's living standard raises, habits and customs change, China's patient of diabetesPerson's number constantly increases, and becomes and is only second to second largest country in the world of India.
At present, for the medicine of diabetes, taboo and bad reaction are more, long-term taking, easily cause hepatotoxicity wind agitation andDigestive tract side effects problem. Therefore from traditional medicine, finding active strong, the low active drug of side effect becomes and addresses this problemNew way. Dai Nationality's drug anesthesia baa cloth (Dischidiaminor (Vahl.) Merr.) is the leaf of Asclepiadaceae plant Dischidia minor (Vahl) Merr, tasteSour, cool in nature. Enter water tower, in Dai Nationality's medicine traditional classical, record its Qinghuo Jiedu, swelling and pain relieving, grow hydrolysis yearningly, cure mainly high hot dry throat mouthYearningly, the diseases such as polydipsia wish drink, also can treat the symptoms such as mumps, submandibular lymph nodes, relieving mammary gland gall; He lid pass through (AspidopterysObcordataHemsl.) for the rattan of the Malpighiaceae plant obcordate aspidopterys stem of falling the heart, lightly seasoned, micro-hardship, cold in nature, enter water tower, function profitWater row stone, clearing heat and detoxicating, tradition is applied to oedema; Bud Nan Ba (PassiflorawilsoniiHemsl.) is PassifloraceaeThe herb of plant sickleleaf passionflower, bitter, property is flat, inlet air, fire, water tower, function compensate qi and blood, damp dispelling and pain relieving, improves the health, and passesSystem is applied to the diseases such as soreness and weakness of waist and knees, impotence; Guest bright (Clerodendrumjaponicum (Thunb.) Sweet) is VerenaceaePlant is remitted root and the leaf of paulownia, and taste is sweet, and property is flat, enters water, Feng Ta, and effect is clearing heat and detoxicating, cooling blood and hemostasis, and logical newborn lactogenesis, menstruction regulating and pain relieving,Propose gas-solid de-, strengthening the muscles and bones, the disease such as tradition is applied to limbs fatigue, soreness and weakness of waist and knees. Four kinds of main inlet airs of Dai Nationality's medicine, water tower, by certainRatio can strengthen its cooling blood and hemostasis after mixing, and grows the thirsty effect of hydrolysis, can be used for treating the diseases such as polydipsia wish drink, and we pass through medicineEffect is learned screening and is found, can obviously reduce hyperglycemic rat blood glucose value by extracting the extract obtaining after compatibility, and effect is existingAt 2-5 times of use medicine melbine, there is consumption low, the effect of successful.
Summary of the invention
The object of the present invention is to provide from Dai Nationality's drug anesthesia baa cloth (Dischidiaminor (Vahl.) Merr.), he lid pass through(AspidopterysobcordataHemsl.), bud Nan Ba (PassiflorawilsoniiHemsl.), guest are brightIn (Clerodendrumjaponicum (Thunb.) Sweet), extract a kind of extract with hypoglycemic activity.
Another object of the present invention is to provide the method for this extract of preparation.
Another object of the present invention is to propose this extract and has in preparation pharmaceutical preparation and the health care of hypoglycemic activityApplication in food.
In order to achieve the above object, the present invention has taked following technical scheme:
(1) get numb baa cloth (Dischidiaminor (Vahl.) Merr.) 20-50 part, he lid pass through (AspidopterysObcordataHemsl.) 10-30 part, bud Nan Ba (PassiflorawilsoniiHemsl.) 20-40 part, guest are bright(Clerodendrumjaponicum (Thunb.) Sweet) 10-30 part, after air drying, co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add quality of medicinal material 10-30 70% ethanol doubly, heating and refluxing extraction,Extraction time is 1-3 time.
(3) get in (2) and extract the liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60DEG C, finally obtain the medicinal extract that relative density is 1.15-1.5.
(4) get the medicinal extract obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, is splined onIn silicagel column, applied sample amount is calculated as 20-40 doubly with medicinal extract amount and the ratio of silica gel amount, and Static Adsorption 30 minutes, then uses 10 successively50% ethanol of the water of individual column volume, 20% ethanol of 10 column volumes and 10 column volumes carries out wash-out, collects 50% ethanolEluent.
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 DEG C,Finally obtain the medicinal extract that relative density is 1.15-1.5.
(6) get the medicinal extract obtaining in (5), carry out drying under reduced pressure in vacuum drying chamber, heating-up temperature is no more than 60 DEG C, dryDry rear pulverizing. Obtain extract provided by the invention.
As the improvement to technique scheme and supplement:
(7), when the medicinal powder obtaining in to (1) extracts, extracting solvent can be 30%-100% ethanol.
(8), when the medicinal powder obtaining in to (1) extracts, extracting mode can be heating and refluxing extraction, ultrasonicExtract or diacolation extraction.
(9), when the medicinal extract obtaining carries out silica gel enrichment, use the silica gel into middle polarity in to (3).
The extract preparing by technique scheme is the plant extracts of the pure natural of dietotherapeutic, subsequentlyEffect experiment will prove, extract of the present invention has significant hypoglycemic damaging action.
The invention still further relates to using extract of the present invention as active component, add the medicine of conventional medicine excipient or assistant agentComposition. Conventionally the extract of the present invention that pharmaceutical composition of the present invention contains 0.1-95% weight.
The pharmaceutical composition of extract of the present invention can be according to method preparation well known in the art. Except extract of the present inventionIn the scope of not overslaugh effect of the present invention, can suitably be mixed for as required common pharmaceutical preparation and health care food outward,For example, in other compositions of product: PVPP, carboxyrnethyl starch sodium, microcrystalline cellulose, lactose, aspartame, hardIn fatty acid magnesiums etc., add auxiliary material, additional auxiliary material and dressing auxiliary material, its formulation can be any pharmaceutically said formulation, preferablyTablet, pill, capsule or oral liquid.
Detailed description of the invention
Below in conjunction with specific embodiment, further set forth the present invention, these embodiment are interpreted as only for this is describedBright and be not used in and limit the scope of the invention, after having read the content that the present invention records, those skilled in the art canSo that the present invention is made various changes or modifications, these equivalences change and modify and fall into equally that the claims in the present invention book limitsScope.
The preparation of embodiment 1 Dai Nationality's medicament extract
(1) get 20 parts of numb baa cloth (Dischidiaminor (Vahl.) Merr.), he lid pass through (AspidopterysObcordataHemsl.) 10 parts, 20 parts of bud Nan Ba (PassiflorawilsoniiHemsl.), guest are bright(Clerodendrumjaponicum (Thunb.) Sweet) 10 parts, co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 30% ethanol of 10 times of quality of medicinal materials, heating and refluxing extraction, extractsNumber of times is 1 time.
(3) get in (2) and extract the liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60DEG C, finally obtain relative density and be 1.15 medicinal extract.
(4) get the medicinal extract obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, is splined onIn silicagel column, applied sample amount is calculated as 20 times with medicinal extract amount and the ratio of silica gel amount, and then Static Adsorption 30 minutes uses 10 posts successivelyThe water of volume, 20% ethanol of 10 column volumes, collect 20% ethanol eluate,
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 DEG C,Finally obtain relative density and be 1.15 medicinal extract.
(6) get the medicinal extract obtaining in (5), carry out drying under reduced pressure in vacuum drying chamber, heating-up temperature is no more than 60 DEG C, dryDry rear pulverizing. Obtain extract provided by the invention.
The preparation of embodiment 2 Dai Nationality's medicament extracts
(1) get 50 parts of numb baa cloth (Dischidiaminor (Vahl.) Merr.), he lid pass through (AspidopterysObcordataHemsl.) 30 parts, 40 parts of bud Nan Ba (PassiflorawilsoniiHemsl.), guest are bright(Clerodendrumjaponicum (Thunb.) Sweet) 30 parts, co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 100% ethanol of 10 times of quality of medicinal materials, ultrasonic extraction, extracts inferiorNumber is 3 times.
(3) get in (2) and extract the liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60DEG C, finally obtain relative density and be 1.5 medicinal extract.
(4) get the medicinal extract obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, is splined onIn silicagel column, applied sample amount is calculated as 40 times with medicinal extract amount and the ratio of silica gel amount, and then Static Adsorption 30 minutes uses 10 posts successively30% ethanol of the water of volume, 10% ethanol of 10 column volumes, 20% ethanol of 10 column volumes, 10 column volumes and 1050% ethanol of column volume carries out wash-out, collects 50% ethanol eluate,
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 DEG C,Finally obtain relative density and be 1.5 medicinal extract.
(6) get the medicinal extract obtaining in (5), carry out drying under reduced pressure in vacuum drying chamber, heating-up temperature is no more than 60 DEG C, dryDry rear pulverizing. Obtain extract provided by the invention.
The preparation of embodiment 3 Dai Nationality's medicament extracts
(1) get 30 parts of numb baa cloth (Dischidiaminor (Vahl.) Merr.), he lid pass through (AspidopterysObcordataHemsl.) 20 parts, 30 parts of bud Nan Ba (PassiflorawilsoniiHemsl.), guest are bright(Clerodendrumjaponicum (Thunb.) Sweet) 20 parts, co-grinding, crosses No. 2 sieves of pharmacopeia.
(2) get the medicinal powder obtaining in (1), add 70% ethanol of 10 times of quality of medicinal materials, ultrasonic extraction, extraction timeIt is 2 times.
(3) get in (2) and extract the liquid obtaining, in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60DEG C, finally obtain relative density and be 1.28 medicinal extract.
(4) get the medicinal extract obtaining in (3), the water dilution that adds 5 times of quality mixes and mixes thoroughly with isopyknic gel afterwards, is splined onIn silicagel column, applied sample amount is calculated as 30 times with medicinal extract amount and the ratio of silica gel amount, and then Static Adsorption 30 minutes uses 10 posts successively30% ethanol of the water of volume, 10% ethanol of 10 column volumes, 20% ethanol of 10 column volumes, 10 column volumes and 1040% ethanol of column volume carries out wash-out, collects 40% ethanol eluate,
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, condensed water bath temperature is no more than 60 DEG C,Finally obtain relative density and be 1.2 medicinal extract.
(6) get the medicinal extract obtaining in (5), carry out drying under reduced pressure in vacuum drying chamber, heating-up temperature is no more than 60 DEG C, dryDry rear pulverizing, obtains extract provided by the invention.
Case study on implementation 4 (Dai Nationality's medicament extract function of blood sugar reduction research)
1, extract preparation
Be prepared according to preparation method in embodiment 3.
2, animal gavage
Get 40 of small white mouses, body weight 20 ± 2g, male and female half and half, separately feed, after empty stomach 24h, lumbar injection four pyrimidines200mg/kg, causes Experimental diabetic model, after 48h., is divided into four groups, and 10 every group, male and female half and half, and carry out gavage, connectContinuous gavage 5 days. Gavage dosage is as follows:
First group of blank group, gavage physiological saline
Second group of hyperglycemia model group, gavage physiological saline
The 3rd group of positive controls, gavage melbine 250mg/kgd
The 4th group of Dai Nationality's medicament extract low concentration group, gavage 100mg/kgd
The 5th group of Dai Nationality's medicament extract high concentration group, gavage 250mg/kgd
3, get blood
18h. after the 5th gavage, plucks eyeball by each six (every group each 1) and gets blood (0.3mL/ only), and oftenIn test tube, add in advance anticoagulant trisodium citrate 0.1g. After shaking up, add 6mol/L sulfuric acid 5mL and 10% sodium tungstate0.7mL, shake up process 10min, after with the centrifugal 15min of 3500r/min, obtain supernatant and be serum.
4, measure blood-sugar content
Adopt ortho-aminotoluene method to measure (Xu Shuyun, 1991).
5, result
By more known, Dai Nationality's medicament extract high dose group can obviously reduce the diabetes model being caused by alloxanSmall white mouse blood sugar concentration, concrete impact the results are shown in Table 1.
Table 1 Dai Nationality medicament extract is to experimental hyperglycemia small white mouse blood sugar influence result
Claims (7)
1. Dai Nationality's medicament extract, is characterized in that, described Dai Nationality medicament extract by 30 parts of medicinal material fiber crops baa cloth, he lid pass through 20 parts, budBright 20 parts of 30 parts, nanmu dam, guest make.
2. a method of preparing the Dai Nationality claimed in claim 1 medicament extract, comprising: take 30 parts of medicinal material fiber crops baa cloth, he lid pass through20 parts, 30 parts, bud nanmu dam, bright 20 parts of guest; After medicinal material air drying, pulverize; After mixing, be 30%-100% alcohol immersion by concentrationRear extraction obtains liquid; Extract concentrates to obtain medicinal extract; Medicinal extract utilizes silicagel column to carry out enrichment; Make powder.
3. method according to claim 2, is characterized in that, adopts heating and refluxing extraction, ultrasonic extraction or diacolation to extractObtain liquid.
4. method according to claim 2, is characterized in that, described powder is by reducing pressure in vacuum drying chamberDry, crushed after being dried makes.
5. method according to claim 2, is characterized in that, when medicinal extract utilizes silicagel column to carry out enrichment, eluting solvent isThe ethanol of variable concentrations, according to concentration of alcohol wash-out successively from low to high.
6. a Dai Nationality as claimed in claim 1 medicament extract or prepare according to the method for any one in claim 2-5Described Dai Nationality medicament extract is applied preparing in hypoglycemic pharmaceutical preparation and health food.
7. an application claimed in claim 6, is characterized in that, this pharmaceutical preparation is tablet, capsule, granule or ballAgent.
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| 8种傣药提取物体外抑制α-糖苷酶活性的研究;胡鑫等;《中南民族大学学报(自然科学版)》;20130930;第32卷(第3期);第60-62页及第117页 * |
| 西双版纳布朗族民间复方录;彭朝忠等;《中国民族民间医药杂志》;20070228(第1期，总第84期);第58页 * |
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