CN101152213A - External spermatozoon nutrient solution of ganoderma lucidum, method for preparing the same and application of the same - Google Patents
External spermatozoon nutrient solution of ganoderma lucidum, method for preparing the same and application of the same Download PDFInfo
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- CN101152213A CN101152213A CNA2007101131151A CN200710113115A CN101152213A CN 101152213 A CN101152213 A CN 101152213A CN A2007101131151 A CNA2007101131151 A CN A2007101131151A CN 200710113115 A CN200710113115 A CN 200710113115A CN 101152213 A CN101152213 A CN 101152213A
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Abstract
The invention discloses a mythic fungus in vitro sperm nutrient solution, and the preparation method and application of the solution. The solution is mixed by polysaccharide extracted from mythic fungus raw material and normal saline, wherein the ratio between the mythic fungus polysaccharide and the normal saline is that 1 gram to 10 millimeter. The preparation method is: mythic fungus raw material is cleaned, dried and smashed, and is drenched in normal saline; then the soak is put in a vessel, heated under conditions between 95 to 105 degrees centigrade, and filtered to produce mythic fungus extract. The application of the mythic fungus in vitro sperm nutrient solution is: the poria cocos in vitro sperm nutrient solution is mixed uniformly with semen with a liquid volume ratio of 1 to 1 to cultivate sperm and activated sperm. The invention is capable of enhancing the sperm mobility by 35 to 56 percent without any damage to or side effects on the sperm, so the product with such high effects can replace imported product. Besides, the price is greatly reduced compared with imported goods.
Description
Technical field
The present invention relates to sperm nutrient solution, is a kind of external spermatozoon nutrient solution of ganoderma lucidum and preparation method thereof and application.
Background technology
Because low sperm activity and infertile patients with infertility accounts for the large percentage of patients with infertility, it is a lot of to treat this class infertility oral drugs kind, but, no matter Western medicine or Chinese medicine, its curative effect is all not good enough, even some patients needed just can take effect in oral several years, causes bigger financial burden and mental pressure to the patient, and be prone to various senses of discomfort, patient body health there is certain influence.For this reason, the use sperm nutrient solution that began one's study is in the world in recent years cultivated at external vigor to patient's sperm, finally reaches the purpose that strengthens motility of sperm.This nutritional solution of China's use at present is imported product, costs an arm and a leg, and generally all contains the chemicals composition in these nutritional solutions.Yet the ultimate aim of this area research is to use pure natural substance.
Summary of the invention
The objective of the invention is, a kind of external spermatozoon nutrient solution of ganoderma lucidum and preparation method thereof and application are provided, it adopts the pure natural substance Ganoderma is that raw material extracts wherein polysaccharide, makes sperm nutrient solution, makes sperm strengthen motility of sperm in external activation to reach.
The present invention for achieving the above object, be achieved through the following technical solutions: a kind of external spermatozoon nutrient solution of ganoderma lucidum, it is polysaccharide and the blended liquid of normal saline that extracts in the Ganoderma raw material, and the ratio of ganoderan and normal saline is 1 kairine sesame polysaccharide: 10 ml physiological salines.
The preparation method of described a kind of external spermatozoon nutrient solution of ganoderma lucidum, getting the Ganoderma raw material cleans it, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Ganoderma raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains Ganoderma extracting solution;
Ganoderma extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain ganoderma lucidum crude polysaccharide;
Again ganoderma lucidum crude polysaccharide is carried out separation and purification: obtain a ganoderan component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, gets the refining ganoderam lucidum polysaccharide after the vacuum lyophilization;
Add the ratio of 10 milliliters of normal saline according to 1 gram refining ganoderam lucidum polysaccharide, mix homogeneously is made the ganoderan sperm nutrient solution.
Described soak was at vessel in heating 10-45 minute.
The ratio of described Ganoderma raw material and normal saline is 100g: 1000 milliliters.
The application of described a kind of external spermatozoon nutrient solution of ganoderma lucidum, Ganoderma sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
The Ganoderma raw material that the present invention adopts is the food and medicament dual-purpose fungus.Ganoderma has effects such as nourishing, brain-strengthening, brute force, antiinflammatory, diuresis, stomach reinforcing.Pharmacological research shows: Ganoderma has heart tonifying, protects the liver and sedation.Contain polysaccharide, alkaloids, sterols, phenols, aminoacid, mannitol, malicious angle alcohol, lactone, coumarin etc. in the Ganoderma.Medical research shows: the Ganoderma safety non-toxic, have no side effect, have the effect that suppresses tumor, regulate immunologic function, nucleic acid anabolism, effects such as microcirculation improvement, blood pressure lowering, blood fat reducing, removing free radical.
Further studies show that: polysaccharide in the Ganoderma and aminoacid can improve the mobility of sperm.
The present invention adopts the raw material of Ganoderma as external sperm nutrient solution, the sperm nutrient solution of producing is through experimental results show that, can improve sperm motility 35-56%, prolong 24-28 hour sperm in vitro time-to-live, sperm there is not any infringement, without any side effects, the complete import substitutes of its effect, and price reduces significantly than imported product, for China's patients with infertility provides a kind of economical and practical, safety and the outstanding product of obvious results.
The specific embodiment
Embodiment:
External spermatozoon nutrient solution of ganoderma lucidum of the present invention is to adopt polysaccharide and the blended liquid of normal saline that extracts in the Ganoderma raw material, and the ratio of ganoderan and normal saline is 1 kairine sesame polysaccharide: 10 ml physiological salines.
The preparation method of described external spermatozoon nutrient solution of ganoderma lucidum is, getting Ganoderma is that raw material is cleaned it, drying is 2 hours under 60 ℃, pulverize then, insert in 0.8% the normal saline and soaked 1 hour, the ratio of Ganoderma raw material and normal saline is 100g: the 900-1150 milliliter, this ratio can be: 100g: 900 milliliters, 100g: 1150 milliliters or 100g: 1100 milliliters etc. all can, preferred scheme is: the ratio of raw material Ganoderma and normal saline is 100g: 1000 milliliters.Then soak is inserted in the container and heat down at 100 ℃, the taking-up after-filtration removes slag and obtains Ganoderma extracting solution; Ganoderma extracting solution was descended dry 1 hour at 60 ℃, obtain dry thing; According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims, again filtering residue is volatilized behind the solvent with dipping in 250 milliliters of 80% ethanol 10 hours or 12 hours or 15 hours all can, 80 ℃ of-90 ℃ of following reflux, extract, 2 times, each 1.2 hours, obtain filtrate and filtering residue; Again with filtering residue adding distil water 450-550 milliliter, preferred 500 milliliters are soaked after 0.8-1.2 hour 90 ℃ of following warm macerating 40 minutes, filter, and obtain filtrate and filtering residue, again with filtering residue with 250 ml distilled waters 90 ℃ of following warm macerating 30 minutes, filtration obtains filtrate; Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters; Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 60 ℃ of down oven dry, obtain ganoderma lucidum crude polysaccharide; Again ganoderma lucidum crude polysaccharide is carried out separation and purification: obtain a ganoderan component with distilled water eluting cellulose column; Reuse 0-0.-2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, gets the refining ganoderam lucidum polysaccharide after the vacuum lyophilization; Add the ratio of 10 milliliters of normal saline according to 1 gram refining ganoderam lucidum polysaccharide, mix homogeneously is made the ganoderan sperm nutrient solution.
Soak of the present invention is generally in the time of vessel in heating and was advisable in 10-45 minute.
The application of a kind of external spermatozoon nutrient solution of ganoderma lucidum of the present invention is, Ganoderma sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, is used to cultivate sperm and activation sperm, to reach effect of the present invention.
Claims (5)
1. external spermatozoon nutrient solution of ganoderma lucidum, it is characterized in that: it is polysaccharide and the blended liquid of normal saline that extracts in the Ganoderma raw material, and the ratio of ganoderan and normal saline is 1 kairine sesame polysaccharide: 10 ml physiological salines.
2. the preparation method of the described a kind of external spermatozoon nutrient solution of ganoderma lucidum of claim 1, it is characterized in that: get the Ganoderma raw material it is cleaned, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Ganoderma raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains Ganoderma extracting solution;
Ganoderma extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain ganoderma lucidum crude polysaccharide;
Again ganoderma lucidum crude polysaccharide is carried out separation and purification: obtain a ganoderan component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, gets the refining ganoderam lucidum polysaccharide after the vacuum lyophilization;
Add the ratio of 10 milliliters of normal saline according to 1 gram refining ganoderam lucidum polysaccharide, mix homogeneously is made the ganoderan sperm nutrient solution.
3. the preparation method of a kind of external spermatozoon nutrient solution of ganoderma lucidum according to claim 2, it is characterized in that: soak was at vessel in heating 10-45 minute.
4. the preparation method of a kind of external spermatozoon nutrient solution of ganoderma lucidum according to claim 2, it is characterized in that: the ratio of Ganoderma raw material and normal saline is 100g: 1000 milliliters.
5. the application of each described a kind of external spermatozoon nutrient solution of ganoderma lucidum of claim 1-4 is characterized in that: Ganoderma sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
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|---|---|---|---|
| CNA2007101131151A CN101152213A (en) | 2007-09-27 | 2007-09-27 | External spermatozoon nutrient solution of ganoderma lucidum, method for preparing the same and application of the same |
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| CNA2007101131151A CN101152213A (en) | 2007-09-27 | 2007-09-27 | External spermatozoon nutrient solution of ganoderma lucidum, method for preparing the same and application of the same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957701A (en) * | 2015-06-15 | 2015-10-07 | 苏州葛家坞生物科技有限公司 | Female endocrine balance regulating fruit and vegetable ganoderma lucidum beverage and preparation method thereof |
| CN109997844A (en) * | 2019-05-07 | 2019-07-12 | 西北农林科技大学 | Application of the ganoderma lucidum polysaccharide in domestic animal freezing of semen long-term preservation |
-
2007
- 2007-09-27 CN CNA2007101131151A patent/CN101152213A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957701A (en) * | 2015-06-15 | 2015-10-07 | 苏州葛家坞生物科技有限公司 | Female endocrine balance regulating fruit and vegetable ganoderma lucidum beverage and preparation method thereof |
| CN109997844A (en) * | 2019-05-07 | 2019-07-12 | 西北农林科技大学 | Application of the ganoderma lucidum polysaccharide in domestic animal freezing of semen long-term preservation |
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