CN101152212A - Tuckahoe external spermatozoon nutrient solution, method for preparing the same and application of the same - Google Patents
Tuckahoe external spermatozoon nutrient solution, method for preparing the same and application of the same Download PDFInfo
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- CN101152212A CN101152212A CNA2007101131147A CN200710113114A CN101152212A CN 101152212 A CN101152212 A CN 101152212A CN A2007101131147 A CNA2007101131147 A CN A2007101131147A CN 200710113114 A CN200710113114 A CN 200710113114A CN 101152212 A CN101152212 A CN 101152212A
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Abstract
The invention discloses a poria cocos in vitro sperm nutrient solution, the preparation method and the application of the solution. The solution is mixed by polysaccharide extracted from poria cocos raw material and normal saline, wherein the ratio between the poria cocos polysaccharide and the normal saline is that 1 gram to 10 millimeter. The preparation method is: poria cocos raw material is cleaned, dried and smashed, and is drenched in normal saline; then the soak is put in a vessel, heated under conditions between 95 to 105 degrees centigrade, and filtered to produce poria cocos extract. The application of the poria cocos in vitro sperm nutrient solution is: the poria cocos in vitro sperm nutrient solution is mixed uniformly with semen with a liquid volume ratio of 1 to 1 to cultivate sperm and activated sperm. The invention is capable of enhancing the sperm mobility by 35 to 56 percent without any damage or side effects to sperm, so the product with such high effects can replace imported product. Besides, the price is greatly reduced compared with imported goods.
Description
Technical field
The present invention relates to sperm nutrient solution, is a kind of tuckahoe external spermatozoon nutrient solution and preparation method thereof and application.
Background technology
Because low sperm activity and infertile patients with infertility accounts for the large percentage of patients with infertility, it is a lot of to treat this class infertility oral drugs kind, but, no matter Western medicine or Chinese medicine, its curative effect is all not good enough, even some patients needed just can take effect in oral several years, causes bigger financial burden and mental pressure to the patient, and be prone to various senses of discomfort, patient body health there is certain influence.For this reason, the use sperm nutrient solution that began one's study is in the world in recent years cultivated at external vigor to patient's sperm, finally reaches the purpose that strengthens motility of sperm.This nutritional solution of China's use at present is imported product, costs an arm and a leg, and generally all contains the chemicals composition in these nutritional solutions.Yet the ultimate aim of this area research is to use pure natural substance.
Summary of the invention
The objective of the invention is, a kind of tuckahoe external spermatozoon nutrient solution and preparation method thereof and application are provided, its adopts pure natural substance Poria is that raw material extracts wherein polysaccharide, makes sperm nutrient solution, makes sperm strengthen motility of sperm in external activation to reach.
The present invention for achieving the above object, be achieved through the following technical solutions: a kind of tuckahoe external spermatozoon nutrient solution, it is polysaccharide and the blended liquid of normal saline that extracts in the Poria raw material, and the ratio of pachyman and normal saline is 1 gram pachyman: 10 ml physiological salines.
The preparation method of described a kind of tuckahoe external spermatozoon nutrient solution, getting the Poria raw material cleans it, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Poria raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains Poria extracting solution;
Poria extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain brown Poria crude polysaccharides;
Again the Poria crude polysaccharides is carried out separation and purification: obtain a pachyman component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care pachyman 10.9 gram white solids after the vacuum lyophilization;
Make with extra care the ratio that pachyman adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made the pachyman sperm nutrient solution.
Described soak was at vessel in heating 10-45 minute.
The ratio of described Poria raw material and normal saline is 100g: 1000 milliliters.
The application of described a kind of tuckahoe external spermatozoon nutrient solution, Poria sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
Poria raw material of the present invention is a Chinese medicine simply.Its property is flat, sweet in the mouth, light, has the effect of promoting diuresis to eliminate damp pathogen, invigorating the spleen and regulating the stomach, mind tranquilizing and the heart calming.The traditional Chinese medical science is mainly treated edema, deficiency of spleen-QI and stomach-QI with it, fatigue and asthenia and malaise etc.
Modern pharmacology studies show that, the Main Ingredients and Appearance of Poria is polysaccharide and triterpene, Poria has the effect that suppresses tumor, enhance immunity, antiinflammatory, blood vessel dilating, can activate aspects such as local complement, clinical demonstration: the polysaccharide in the Poria has certain curative effect to gastric cancer, nasopharyngeal carcinoma, Poria has no side effect, and is the fungus that a kind of medicine food has both.
Further studies show that: the polysaccharide in the Poria has bigger effect to the activation of external sperm.
The present invention adopts the raw material of Poria as external sperm nutrient solution, the sperm nutrient solution of producing is through experimental results show that, can improve sperm motility 35-56%, prolong 24-28 hour sperm in vitro time-to-live, sperm there is not any infringement, without any side effects, the complete import substitutes of its effect, and price reduces significantly than imported product, for China's patients with infertility provides a kind of economical and practical, safety and the outstanding product of obvious results.
The specific embodiment
Embodiment:
Tuckahoe external spermatozoon nutrient solution of the present invention is to adopt polysaccharide and the blended liquid of normal saline that extracts in the Poria raw material, and the ratio of pachyman and normal saline is 1 gram pachyman: 10 ml physiological salines.
The preparation method of described tuckahoe external spermatozoon nutrient solution is, getting Poria is that raw material is cleaned it, drying is 2 hours under 60 ℃, pulverize then, insert in 0.8% the normal saline and soaked 1 hour, the ratio of Poria raw material and normal saline is 100g: the 900-1150 milliliter, this ratio can be: 100g: 900 milliliters, 100g: 1150 milliliters or 100g: 1100 milliliters etc. all can, preferred scheme is: the ratio of raw material Poria and normal saline is 100g: 1000 milliliters.Then soak is inserted in the container and heat down at 100 ℃, the taking-up after-filtration removes slag and obtains Poria extracting solution; Poria extracting solution was descended dry 1 hour at 60 ℃, obtain dry thing; According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims, again filtering residue is volatilized behind the solvent with dipping in 250 milliliters of 80% ethanol 10 hours or 12 hours or 15 hours all can, 80 ℃ of-90 ℃ of following reflux, extract, 2 times, each 1.2 hours, obtain filtrate and filtering residue; Again with filtering residue adding distil water 450-550 milliliter, preferred 500 milliliters are soaked after 0.8-1.2 hour 90 ℃ of following warm macerating 40 minutes, filter, and obtain filtrate and filtering residue, again with filtering residue with 250 ml distilled waters 90 ℃ of following warm macerating 30 minutes, filtration obtains filtrate; Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters; Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 60 ℃ of down oven dry, obtain the Poria crude polysaccharides; Again the Poria crude polysaccharides is carried out separation and purification: obtain a pachyman component with distilled water eluting cellulose column; Reuse 0-0.-2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care pachyman after the vacuum lyophilization; Make with extra care the ratio that pachyman adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made the pachyman sperm nutrient solution.
Soak of the present invention is generally in the time of vessel in heating and was advisable in 10-45 minute.
The application of a kind of tuckahoe external spermatozoon nutrient solution of the present invention is, Poria sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, is used to cultivate sperm and activation sperm, to reach effect of the present invention.
Claims (5)
1. tuckahoe external spermatozoon nutrient solution, it is characterized in that: it is polysaccharide and the blended liquid of normal saline that extracts in the Poria raw material, the ratio of pachyman and normal saline is 1 gram pachyman: 10 ml physiological salines.
2. the preparation method of the described a kind of tuckahoe external spermatozoon nutrient solution of claim 1, it is characterized in that: get the Poria raw material it is cleaned, descended dry 1.5-2.5 hour at 50 ℃-65 ℃, pulverize then, insert in 0.8% the normal saline and soaked 0.8-1.5 hour, the ratio of Poria raw material and normal saline is 100g: the 900-1150 milliliter, and then soak to be inserted in the container and heat down at 95-105 ℃, the taking-up after-filtration removes slag and obtains Poria extracting solution;
Poria extracting solution was descended dry 1.5-2.5 hour at 50 ℃-60 ℃, obtain dry thing;
According to the ratio of the dry thing of 100 grams than 250 millimeters petroleum ether, dry thing is inserted in the container, 60 ℃-90 ℃ defats in 1 hour that reflux down, filter, petroleum ether reclaims;
Flooded 10-15 hour in the 220-260 milliliter with 80% ethanol after again filtering residue being volatilized solvent,, each 1-2 hour, obtain filtrate and filtering residue 80 ℃ of-90 ℃ of following reflux, extract, 2 times;
Again filtering residue adding distil water 450-550 milliliter is soaked after 0.8-1.2 hour at 90 ℃ of following warm macerating 30-48 minutes, filter, obtain filtrate and filtering residue;
Use the 220-260 ml distilled water at 90 ℃ of following warm macerating 25-35 minutes filtering residue again, filter, obtain filtrate;
Above-mentioned filtrate is merged, be evaporated to 75 milliliters, deproteinising adds 1% activated carbon decolorizing 2 times, sucking filtration, and filtrate adds 95% ethanol to be made and contains the alcohol amount and reach 80%, leaves standstill under 4-12 ℃ 10-15 hour, filters;
Again with residue successively with behind 95% ethanol, dehydrated alcohol, ether, the washing with acetone 50-60 ℃ of down oven dry, obtain brown Poria crude polysaccharides;
Again the Poria crude polysaccharides is carried out separation and purification: obtain a pachyman component with distilled water eluting cellulose column;
Reuse 0-0.2 mol sodium hydroxide carries out gradient elution, collects the polysaccharide eluent, and dialysis is 68-76 hour in flowing water, must make with extra care pachyman 10.9 gram white solids after the vacuum lyophilization;
Make with extra care the ratio that pachyman adds 10 milliliters of normal saline according to 1 gram, mix homogeneously is made the pachyman sperm nutrient solution.
3. the preparation method of a kind of tuckahoe external spermatozoon nutrient solution according to claim 2, it is characterized in that: soak was at vessel in heating 10-45 minute.
4. the preparation method of a kind of tuckahoe external spermatozoon nutrient solution according to claim 2, it is characterized in that: the ratio of Poria raw material and normal saline is 100g: 1000 milliliters.
5. the application of each described a kind of tuckahoe external spermatozoon nutrient solution of claim 1-4 is characterized in that: Poria sperm nutrient solution and seminal fluid is even by 1: 1 mixed of liquid volume, be used to cultivate sperm and activation sperm.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101131147A CN101152212A (en) | 2007-09-27 | 2007-09-27 | Tuckahoe external spermatozoon nutrient solution, method for preparing the same and application of the same |
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| CNA2007101131147A CN101152212A (en) | 2007-09-27 | 2007-09-27 | Tuckahoe external spermatozoon nutrient solution, method for preparing the same and application of the same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547792A (en) * | 2016-01-07 | 2016-05-04 | 山东省计划生育科学技术研究所 | Method for simultaneously detecting spermatoblast apoptosis and membrane integrity |
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2007
- 2007-09-27 CN CNA2007101131147A patent/CN101152212A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547792A (en) * | 2016-01-07 | 2016-05-04 | 山东省计划生育科学技术研究所 | Method for simultaneously detecting spermatoblast apoptosis and membrane integrity |
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