CN101138325B - Garlic test tube high-efficiency inducement culture medium - Google Patents
Garlic test tube high-efficiency inducement culture medium Download PDFInfo
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- CN101138325B CN101138325B CN2007101331016A CN200710133101A CN101138325B CN 101138325 B CN101138325 B CN 101138325B CN 2007101331016 A CN2007101331016 A CN 2007101331016A CN 200710133101 A CN200710133101 A CN 200710133101A CN 101138325 B CN101138325 B CN 101138325B
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- garlic
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Abstract
The present invention discloses a culture medium for high-efficiency inducement of tube garlic belonging to the garlic tissue culture technique. The culture medium is formed by dissolving the 6-benzylpurine 6-BA, adenine AD, alpha-naphthyl acetic acid NAA, cane sugar and agar into the basic culture medium 1/2B5 and the quantity of every part is that 1/2B5 1l, 0.01-0.1mg of 6-BA, 0.05-0.5mg of NAA, 10-30mg of AD, 40g of cane sugar and 6g of agar. The active effects of the present invention are that the tube garlic induced with high efficiency from the garlic tube seedling provides technical basis for virus-free garlic seedlings production in factory. The inducement rate of the tube garlic with the culture medium reaches 91 percent.
Description
Technical field
The invention belongs to the garlic tissue culture technique, particularly induce the foster base of test tube garlic stem from the garlic test-tube plantlet.
Background technology
It is by being transplanted to the land for growing field crops after the hardening that the garlic shoot apical meristem is cultivated inductive test-tube plantlet cultivation common method, and this method is time-consuming and efficient is low, because the test-tube seedling transplanting surviving rate is not high, the production cycle is long, and utility value is little in the production.In the group training detoxifying fast breeding of a lot of plants, expand numerously by in test tube, inducing propagulum, can reach the effect of getting twice the result with half the effort.For example potato carries out the production of virus-free seed potato by the method that induces test tube bulbs.
Summary of the invention
The objective of the invention is to overcome that garlic test-tube seedling transplanting difficulty survives, the difficult inductive problem of test tube garlic, a kind of substratum of garlic test tube high-efficiency inducement is provided, be used for the group training detoxifying fast breeding of garlic.
Technical scheme of the present invention is as follows: this garlic test tube inductive substratum is that 6-benzyl purine 6-BA, VITAMIN B4 AD, α-naa NAA, sucrose, agar are dissolved in minimum medium 1/2B
5In form, the consumption of its each component is: with 1L minimum medium 1/2B
5The add-on meter:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01-0.1mg
α-naa NAA 0.05-0.5mg
VITAMIN B4 AD 20-30mg
Sucrose 40g
Agar 6g.
In the above-mentioned raw materials, 1/2B
5Be minimum medium, cultured tissue grow required inorganic nutritive element and part organotrophy element are provided; 6-benzyl purine 6-BA, α-naa α-NAA, VITAMIN B4 AD is plant-growth regulator, has the effect that promotes that the cultured tissue organ forms; Sucrose provide cultured tissue grow carbon nutrition; The main effect of agar is to solidify substratum, plays a supportive role.
The consumption of above-mentioned each component is that the contriver tests in a large number to grope to sum up on the basis of theory analysis and draws, and prepares substratum according to the above ratio, has and better induces effect.
The optimal medium of above-mentioned inducing garlic bud clumps is:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 40g
Agar 6g.
The making method of above-mentioned substratum is that 6-BA, NAA, AD, sucrose, agar are dissolved in 1/2B
5, stirring, pH value is adjusted between the 5.8-6.0 and gets final product.
Positively effect of the present invention is: utilize garlic test-tube plantlet high-level efficiency to induce the test tube garlic, be easy to batch production and produce garlic detoxification kind garlic.Utilize the inductivity of basal culture medium test tube garlic to reach 91%.
Embodiment
Further set forth beneficial effect of the present invention by the following examples:
Embodiment 1:
Culture medium prescription:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.1mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 30g
Agar 6g.
Embodiment 2:
Culture medium prescription:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 40g
Agar 6g.
Embodiment 3:
Culture medium prescription:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.5mg
VITAMIN B4 AD 30mg
Sucrose 50g
Agar 6g.
Embodiment 4:
Culture medium prescription:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 60g
Agar 6g.
Test method and result:
The substratum of the foregoing description is used for the induction experiment of test tube garlic:
Material: the test-tube plantlet that garlic Xuzhou white garlic shoot apical meristem induces.
Method: the little test-tube plantlet tube of difference in size that garlic Xuzhou white garlic shoot apical meristem is induced is inoculated on the substratum, every test tube is inoculated the test-tube plantlet that 1 strain is induced by shoot apical meristem, placed 22 ℃, the about 2000Lux of light intensity, illumination every day 16 hours, cultivate 10 week the back statisticses see the following form:
The test tube garlic induces the result to add up (4 week)
Drawn as drawing a conclusion by interpretation of result in the table: the inductivity of test tube garlic is 62% among the embodiment 1, but Boiling tube garlic rate low be 42%; The inductivity of test tube garlic is up to 91% in the example 2, and Boiling tube garlic inductivity also is up to 75%; The inductivity of test tube garlic is 66% among the embodiment 3, and the inductivity of Boiling tube garlic is 56%;
The inductivity of test tube garlic is 76.5% among the embodiment 4, and the inductivity of Boiling tube garlic is 61%.
Comprehensive above the analysis, the optimal medium that draws inducing garlic bud clumps is:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 40g
Agar 6g.
Claims (2)
1. the substratum of a garlic test tube high-efficiency inducement, it is characterized in that: it is that 6-benzyl purine 6-BA, VITAMIN B4 AD, α-naa NAA, sucrose and agar are dissolved in minimum medium 1/2B
5In form, the consumption of its each component is:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01-0.1mg
α-naa NAA 0.05-0.5mg
VITAMIN B4 AD 20-30mg
Sucrose 40g
Agar 6g.
2. the substratum of garlic test tube high-efficiency inducement according to claim 1, it is characterized in that: the consumption of its each component of substratum is:
Minimum medium 1/2B
51L
6-benzyl purine 6-BA 0.01mg
α-naa NAA 0.05mg
VITAMIN B4 AD 20mg
Sucrose 40g
Agar 6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101331016A CN101138325B (en) | 2007-09-20 | 2007-09-20 | Garlic test tube high-efficiency inducement culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101331016A CN101138325B (en) | 2007-09-20 | 2007-09-20 | Garlic test tube high-efficiency inducement culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101138325A CN101138325A (en) | 2008-03-12 |
| CN101138325B true CN101138325B (en) | 2010-06-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2007101331016A Expired - Fee Related CN101138325B (en) | 2007-09-20 | 2007-09-20 | Garlic test tube high-efficiency inducement culture medium |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1857065A (en) * | 2006-05-26 | 2006-11-08 | 江苏徐淮地区徐州农业科学研究所 | Culture medium of garlic embryonic callus induction |
| CN1884488A (en) * | 2006-05-26 | 2006-12-27 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing garlic bud clumps |
| CN1884489A (en) * | 2006-05-26 | 2006-12-27 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing garlic somatic embryo |
-
2007
- 2007-09-20 CN CN2007101331016A patent/CN101138325B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1857065A (en) * | 2006-05-26 | 2006-11-08 | 江苏徐淮地区徐州农业科学研究所 | Culture medium of garlic embryonic callus induction |
| CN1884488A (en) * | 2006-05-26 | 2006-12-27 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing garlic bud clumps |
| CN1884489A (en) * | 2006-05-26 | 2006-12-27 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing garlic somatic embryo |
Non-Patent Citations (4)
| Title |
|---|
| 杜永芹，朱建忠，沈卫平.嘉定白蒜茎尖脱毒技术研究.上海农业学报20 1.2004,20(1),9-12. |
| 杜永芹,朱建忠,沈卫平.嘉定白蒜茎尖脱毒技术研究.上海农业学报20 1.2004,20(1),9-12. * |
| 陆关成，李方.余姚白蒜组织培养脱毒的研究.浙江农业学报3 3.1991,3(3),133-137. |
| 陆关成,李方.余姚白蒜组织培养脱毒的研究.浙江农业学报3 3.1991,3(3),133-137. * |
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| CN101138325A (en) | 2008-03-12 |
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