CN1884488A - Culture medium for inducing garlic bud clumps - Google Patents
Culture medium for inducing garlic bud clumps Download PDFInfo
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- CN1884488A CN1884488A CN 200610085915 CN200610085915A CN1884488A CN 1884488 A CN1884488 A CN 1884488A CN 200610085915 CN200610085915 CN 200610085915 CN 200610085915 A CN200610085915 A CN 200610085915A CN 1884488 A CN1884488 A CN 1884488A
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- garlic
- bud
- culture medium
- sucrose
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Abstract
The invention discloses a culture medium induced by garlic bushy bud, which is prepared by dissolving 6-benzyl purine 6-BA, kinetin KT, heteroauxin IAA, casein hydrolysate CH, sucrose and gelose in minimum medium MS. Using the said culture medium, the inductivity of the garlic idiosome can reach 89.0 % and the propagating coefficient of the garlic stem culture can be improved, which is good for industrial production of garlic assembly bud.
Description
Technical field
The invention belongs to the garlic tissue culture technique, particularly from inducing garlic somatic embryo go out the to grow thickly substratum of bud.
Background technology
The common method that the garlic shoot apical meristem is cultivated breeding is that a shoot apical meristem is induced and differentiated a bud, and this kind method is time-consuming and efficient is low, and utility value is little in the production.In the tissue culture fast-propagation of a lot of plants, all utilize the technology that induces the bud of growing thickly to improve breeding coefficient, to reach the effect of getting twice the result with half the effort.But what have now is still on the low side from inducing garlic somatic embryo go out the to grow thickly breeding coefficient of bud.
Summary of the invention
The objective of the invention is to overcome the low problem of breeding coefficient in the cultivation of garlic stem-tip tissue, provide a kind of garlic bud clumps efficient inductive substratum, be used for the tissue culture fast-propagation of garlic.Utilization induces embryo callus, body embryo earlier from a garlic shoot apical meristem, induces the bud of growing thickly at last, obtains a large amount of homogeneity clones, improves the breeding coefficient of garlic stem tip culture, produces the garlic tissue cultured seedling for batch production technical basis is provided.
Technical scheme of the present invention is as follows: the substratum of this inducing garlic bud clumps is dissolved in 6-benzyl purine 6-BA, kinetin KT, indolylacetic acid IAA, caseinhydrolysate CH, sucrose, agar among the minimum medium MS and forms, and the consumption of its each component is: in 1L minimum medium MS add-on:
MS 1L
6-BA 2-4mg
KT 1-2mg
IAA 0.03-0.6mg
CH 200mg
Sucrose 20g
Agar 6g
Be preferably:
MS 1L
6-BA 2mg
KT 1mg
IAA 0.3mg
CH 200mg
Sucrose 20g
Agar 6g
In the above-mentioned raw materials, MS is a minimum medium, and cultured tissue grow required inorganic nutritive element and part organotrophy element are provided; 6-benzyl purine 6-BA, kinetin KT and indolylacetic acid IAA are plant-growth regulator, have to promote cultured tissue to form the effect of the bud of growing thickly; Caseinhydrolysate CH provides organic polypeptide class nutritive element; Sucrose provide cultured tissue grow carbon nutrition; The main effect of agar is to solidify substratum, plays a supportive role.
The consumption of above-mentioned each component is that the contriver tests in a large number to grope to sum up on the basis of theory analysis and draws, and prepares substratum according to the above ratio, has and well induces effect.
The making method of above-mentioned substratum is that 6-BA, KT, IAA, CH, sucrose, agar are dissolved in MS, stirs, and pH value is adjusted between the 5.8-6.0 and gets final product.
Positively effect of the present invention is: utilize the inductivity of basal culture medium garlic somatic embryo to be up to 89.0%, one shoot apical meristem and can induce 14 buds.
Embodiment:
Further set forth beneficial effect of the present invention by the following examples:
Embodiment 1:
Culture medium prescription:
MS 1L
6-BA 2mg
KT 2mg
IAA 0.4mg
CH 200mg
Sucrose 20g
Agar 6g
Embodiment 2:
Culture medium prescription:
MS 1L
6-BA 4mg
KT 2mg
IAA 0.6mg
CH 200mg
Sucrose 20g
Agar 6g
Embodiment 3:
Culture medium prescription:
MS 1L
6-BA 2mg
KT 1mg
IAA 0.3mg
CH 200mg
Sucrose 20g
Agar 6g
Embodiment 4:
Culture medium prescription:
MS 1L
6-BA 2mg
KT 1mg
IAA 0.03mg
CH 200mg
Sucrose 20g
Agar 6g
Test method and result:
The substratum of the foregoing description is used for the induction experiment of garlic bud clumps:
Material: the body embryo that garlic Xuzhou white garlic shoot apical meristem induces.
Method: the body embryo tube that garlic Xuzhou white garlic shoot apical meristem is induced is inoculated on the substratum, body embryo tissue and substratum will be in conjunction with closely, every test tube is inoculated 1 by shoot apical meristem growth somatic embryo agglomerate, placed 25 ℃, the about 2000Lux of light intensity, illumination every day 12 hours, cultivate 4 week the back statisticses see the following form:
Inducing garlic somatic embryo rate and body embryo quantity (4 week)
Drawn as drawing a conclusion by interpretation of result in the table: the inductivity of bud is 30.4% among the embodiment 1, and seedling is sturdy, but the speed of growth is slower; The inductivity of bud is 43.9% in the example 2, the seedling stalwartness, but the speed of growth is slow; The inductivity of bud is 89.0% among the embodiment 3, seedling stalwartness, fast growth; The inductivity of bud is 63.3% among the embodiment 4, and seedling is very thin, fast growth.
Comprehensive above the analysis, the optimal medium that draws inducing garlic bud clumps is:
MS 1L
6-BA 2mg
KT 1mg
IAA 0.3mg
CH 200mg
Sucrose 20g
Agar 6g.
Claims (2)
1, a kind of substratum of inducing garlic bud clumps, it is characterized in that 6-benzyl purine 6-BA, kinetin KT, indolylacetic acid IAA, caseinhydrolysate CH, sucrose, agar are dissolved among the minimum medium MS and make, the consumption of its each component is: in 1L minimum medium MS add-on:
MS 1L
6-BA 2-4mg
KT 0.03-0.6mg
IAA 0.3mg
CH 200mg
Sucrose 20g
Agar 6g.
2, the substratum of the described inducing garlic bud clumps of a kind of claim 1 is characterized in that the consumption of each component is:
MS 1L
6-BA 2mg
KT 1mg
IAA 0.3mg
CH 200mg
Sucrose 20g
Agar 6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100859152A CN100420740C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic bud clumps |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100859152A CN100420740C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic bud clumps |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1884488A true CN1884488A (en) | 2006-12-27 |
| CN100420740C CN100420740C (en) | 2008-09-24 |
Family
ID=37582795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100859152A Expired - Fee Related CN100420740C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic bud clumps |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100420740C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138325B (en) * | 2007-09-20 | 2010-06-09 | 江苏徐淮地区徐州农业科学研究所 | Garlic test tube high-efficiency inducement culture medium |
| CN101851601A (en) * | 2010-05-06 | 2010-10-06 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing somatic embryos from garlic embryonic suspension culture system |
| CN104521754A (en) * | 2014-12-10 | 2015-04-22 | 湖北省农业科学院经济作物研究所 | Rapid propagation method of Allium ovalifolium on high mountain |
| CN107455391A (en) * | 2017-08-15 | 2017-12-12 | 河南省农业科学院园艺研究所 | A kind of grafting activator and the method for improving graft survival rate |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090901C (en) * | 1999-06-15 | 2002-09-18 | 南京农业大学 | Method for quickly multiplying detoxicated garlic seedlings as seed |
| CN100456922C (en) * | 2005-12-15 | 2009-02-04 | 上海交通大学 | Method for producing detoxified sprout by cultivating garlic stem tip combined with cold treatment |
-
2006
- 2006-05-26 CN CNB2006100859152A patent/CN100420740C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138325B (en) * | 2007-09-20 | 2010-06-09 | 江苏徐淮地区徐州农业科学研究所 | Garlic test tube high-efficiency inducement culture medium |
| CN101851601A (en) * | 2010-05-06 | 2010-10-06 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing somatic embryos from garlic embryonic suspension culture system |
| CN104521754A (en) * | 2014-12-10 | 2015-04-22 | 湖北省农业科学院经济作物研究所 | Rapid propagation method of Allium ovalifolium on high mountain |
| CN104521754B (en) * | 2014-12-10 | 2016-06-15 | 湖北省农业科学院经济作物研究所 | High mountain ovum leaf fragrant-flowered garlic method for quickly breeding |
| CN107455391A (en) * | 2017-08-15 | 2017-12-12 | 河南省农业科学院园艺研究所 | A kind of grafting activator and the method for improving graft survival rate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100420740C (en) | 2008-09-24 |
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| SE01 | Entry into force of request for substantive examination | ||
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Granted publication date: 20080924 |