CN101138324B - Culture medium for inducing clumping bud of sweet potato - Google Patents
Culture medium for inducing clumping bud of sweet potato Download PDFInfo
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- CN101138324B CN101138324B CN2007101326003A CN200710132600A CN101138324B CN 101138324 B CN101138324 B CN 101138324B CN 2007101326003 A CN2007101326003 A CN 2007101326003A CN 200710132600 A CN200710132600 A CN 200710132600A CN 101138324 B CN101138324 B CN 101138324B
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- sweet potato
- culture medium
- agar
- bud
- kinetin
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Abstract
The present invention relates to a culture medium for inducing sweet potato clustered shoots belonging to the sweet potato tissue culture technology. The culture medium is formed by dissolving the kinetin KT, 6-benzyl purine 6-BA, heteroauxing IAA, hydrolyzed casein CH, cane sugar and agar into the basic culture medium MS; the quantity of every part is that MS 11, 1.0-2.0 mg of KT, 1.0-2.0 mg of 6-BA, 0.2-2.0mg of IAA, CH 200mg, 20g of cane sugar and 6g of agar. The active effects of the present invention are that a stem tip meristematic tissue is utilized to induce 8.6 shoots and the inducement rate is 860 percent, which obviously promotes the tissue culture efficiency.
Description
Technical field
The present invention relates to a kind of substratum of inducing clumping bud of sweet potato, belong to the sweet potato tissue culture technique.Particularly induce the substratum of deriving the bud of growing thickly from the sweet potato stem tip meristematic tissue.
Background technology
The common method of tip Meristem Culture of Sweet Potatoes breeding is that a shoot apical meristem is induced and differentiated a bud, and this kind method is time-consuming and efficient is low.In the tissue culture fast-propagation of a lot of plants, all utilize the technology that induces the bud of growing thickly to improve breeding coefficient, reach the effect of getting twice the result with half the effort.
Summary of the invention
The objective of the invention is to overcome the low problem of breeding coefficient in the sweet potato stem tip tissue culture, provide a kind of clumping bud of sweet potato efficient inductive substratum, be used for the tissue culture fast-propagation of sweet potato.
Technical scheme of the present invention is as follows: the substratum of this inducing clumping bud of sweet potato is dissolved in kinetin KT, 6-benzyl purine 6-BA, indolylacetic acid IAA, caseinhydrolysate CH, sucrose and agar among the minimum medium MS and forms, and the consumption of its each component is: in 1L minimum medium MS add-on:
Minimum medium MS 1L
Kinetin KT 1.0-2.0mg
6-benzyl purine 6-BA 1.0-2.0mg
Indolylacetic acid IAA 0.2-2.0mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
In the above-mentioned raw materials, MS is a minimum medium, and cultured tissue grow required inorganic nutritive element and part organotrophy element are provided; Kinetin KT, 6-benzyl purine 6-BA and indolylacetic acid IAA are plant-growth regulator, have to promote cultured tissue to form the effect of the bud of growing thickly; Caseinhydrolysate CH provides organic polypeptide class nutritive element; Sucrose provide cultured tissue grow carbon nutrition; The main effect of agar is to solidify substratum, plays a supportive role.
The consumption of above-mentioned each component is that the contriver tests in a large number to grope to sum up on the basis of theory analysis and draws, and prepares substratum according to the above ratio, all has and better induces effect.
The making method of above-mentioned substratum is that KT, 6-BA, IAA, CH, sucrose, agar are dissolved in MS, stirs, and pH value is adjusted between the 5.8-6.0 and gets final product.
Positively effect of the present invention is: utilize shoot apical meristem of basal culture medium sweet potato can induce 8.6 buds, inducing efficient is 860%, significantly improves group training efficient.Utilization induces the bud of growing thickly from a sweet potato stem tip meristematic tissue, obtains a large amount of homogeneity clones, improves the breeding coefficient that sweet potato stem tip is cultivated, and is easy to batch production and produces the sweet potato tissue cultured seedling.
Embodiment
Further set forth beneficial effect of the present invention by the following examples:
Embodiment 1
Culture medium prescription:
Minimum medium MS 1L
Kinetin KT 1.0mg
6-benzyl purine 6-BA 2.0mg
Indolylacetic acid IAA 2.0mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Embodiment 2:
Minimum medium MS 1L
Kinetin KT 2.0mg
6-benzyl purine 6-BA 2.0mg
Indolylacetic acid IAA 2.0mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Embodiment 3:
Minimum medium MS 1L
Kinetin KT 1.0mg
6-benzyl purine 6-BA 1.0mg
Indolylacetic acid IAA 0.2mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Embodiment 4:
Minimum medium MS 1L
Kinetin KT 1.0mg
6-benzyl purine 6-BA 1.0mg
Indolylacetic acid IAA 2.0mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Test method and result:
The substratum of the foregoing description is used for the induction experiment of clumping bud of sweet potato:
Material: sweet potato Xuzhou 18 shoot apical meristems.
Method: sweet potato Xuzhou 18 shoot apical meristems are inoculated on the substratum, and 1 shoot apical meristem of every test tube inoculation placed 25 ℃, the about 2000Lux of light intensity, illumination every day 12 hours, cultivate 4 weeks afterwards statisticses see the following form:
The sweet potato bud is induced quantity (4 week)
Drawn as drawing a conclusion by interpretation of result in the table: the inductivity of bud is 309% among the embodiment 1, and seedling is sturdy, but the speed of growth is slower; The inductivity of bud is 330% in the example 2, and seedling is healthy and strong, but the speed of growth is slow; The inductivity of bud is 860% among the embodiment 3, and seedling is healthy and strong, fast growth; The inductivity of bud is 466% among the embodiment 4, and seedling is very thin, fast growth.
Comprehensive above the analysis, the optimal medium that draws inducing clumping bud of sweet potato is:
Minimum medium MS 1L
Kinetin KT 1.0mg
6-benzyl purine 6-BA 1.0mg
Indolylacetic acid IAA 0.2mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Claims (2)
1. the substratum of an inducing clumping bud of sweet potato, it is characterized in that: this substratum is dissolved in kinetin KT, 6-benzyl purine 6-BA, indolylacetic acid IAA, caseinhydrolysate CH, sucrose and agar among the minimum medium MS and forms, and the consumption of its each component is:
Minimum medium MS 1L
Kinetin KT 1.0-2.0mg
6-benzyl purine 6-BA 1.0-2.0mg
Indolylacetic acid IAA 0.2-2.0mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
2. the substratum of inducing clumping bud of sweet potato according to claim 1, it is characterized in that: the consumption of substratum each component is:
Minimum medium MS 1L
Kinetin KT 1.0mg
6-benzyl purine 6-BA 1.0mg
Indolylacetic acid IAA 0.2mg
Caseinhydrolysate CH 200mg
Sucrose 20g
Agar 6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101326003A CN101138324B (en) | 2007-09-20 | 2007-09-20 | Culture medium for inducing clumping bud of sweet potato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101326003A CN101138324B (en) | 2007-09-20 | 2007-09-20 | Culture medium for inducing clumping bud of sweet potato |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101138324A CN101138324A (en) | 2008-03-12 |
| CN101138324B true CN101138324B (en) | 2011-01-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101326003A Expired - Fee Related CN101138324B (en) | 2007-09-20 | 2007-09-20 | Culture medium for inducing clumping bud of sweet potato |
Country Status (1)
| Country | Link |
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| CN (1) | CN101138324B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524076B (en) * | 2012-02-10 | 2013-05-22 | 江苏徐州甘薯研究中心 | Method suitable for regenerating weak regeneration gene type sweet potato tips cultivating plant |
| CN103004597A (en) * | 2012-12-17 | 2013-04-03 | 北京市农业技术推广站 | Dedicated culture medium for virus-free meristem plantlets (MS) of 'Yi-Zi 138'sweet potatoes |
| CN103598094B (en) * | 2013-10-31 | 2016-08-17 | 青岛文创科技有限公司 | A kind of abductive approach of Rhizoma Steudnerae Henryanae Multiple Buds |
| CN104885939B (en) * | 2015-05-26 | 2017-01-11 | 福建农林大学 | Chemical disinfection tissue culture method for Ipomoea batatas Lam. |
| CN114431143B (en) * | 2022-02-09 | 2023-07-21 | 廊坊市农林科学院 | Improved MS culture medium and preparation method and application thereof |
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2007
- 2007-09-20 CN CN2007101326003A patent/CN101138324B/en not_active Expired - Fee Related
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| CN101138324A (en) | 2008-03-12 |
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Granted publication date: 20110105 Termination date: 20130920 |