CN100390273C - Culture medium for inducing garlic somatic embryo - Google Patents
Culture medium for inducing garlic somatic embryo Download PDFInfo
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- CN100390273C CN100390273C CNB2006100859167A CN200610085916A CN100390273C CN 100390273 C CN100390273 C CN 100390273C CN B2006100859167 A CNB2006100859167 A CN B2006100859167A CN 200610085916 A CN200610085916 A CN 200610085916A CN 100390273 C CN100390273 C CN 100390273C
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- garlic
- culture medium
- embryo
- agar
- sucrose
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Abstract
The present invention discloses a culture medium for inducing somatic cells from garlic embryonic calluses. The culture medium is prepared by dissolving 6-benzyl purine 6-BA, 2, 4-dichlorophenoxyacetic acid, 2, 4-D, casein hydrolysate CH, sucrose and agar in a basic culture medium MS. The culture medium has the advantage of high inductivity, which is suitable for factory production for garlic.
Description
Technical field
The invention belongs to the garlic tissue culture technique, particularly go out the substratum of somatic embryo from garlic embryonic callus induction.
Background technology
The callus of inducing the explant somatocyte division to form in the plant tissue culture, a class are loose callus (non-embryonic callus tissue), and a class is an embryo callus.Have only embryo callus under suitable condition, just can continue to break up again, form embryoid, and then form bud, root, the whole plant of regenerating.Embryoid also claims somatic embryo or body embryo, is that its growth course and zygotic embryo are similar, are divided into several stages such as proembryo, globular embryo, heart-shape embryo, torpedo-shape embryo and cotyledonary embryos by the similar embryo's of somatocyte generation structure.The embryoid regeneration system rapidly is not only optimal gene transformation receptor system, also is one of effective ways of tissue-culturing quick-propagation.People utilize embryoid that this quick modes of reproduction takes place, at its surface bag with the special organic substance of one deck etc., promptly so-called " synthetic seed " provides new approach for producing seedling fast, but it is lower to go out the inductivity of somatic embryo from garlic embryonic callus induction at present.
Summary of the invention
The objective of the invention is to overcome the garlic stem-tip tissue cultivate in by the low problem of embryo callus body embryonal induction rate, provide a kind of garlic somatic embryo efficient inductive substratum, be used for the research and production of garlic.
Technical scheme of the present invention is as follows: the substratum of this inducing garlic somatic embryo is with 6-benzyl purine 6-BA, 2,4-dichlorphenoxyacetic acid 2,4-D, hydrolysis junket egg CH, sucrose, agar are dissolved in and form among the minimum medium MS, and the consumption of its each component is: in 1L minimum medium MS add-on:
MS 1L
6-BA 0.1-1.0mg
2，4-D 0.1-1.0mg
CH 200mg
Sucrose 20g
Agar 6g.
Be preferably:
MS 1L
6-BA 0.1mg
2，4-D 1.0mg
CH 200mg
Sucrose 20g
Agar 6g
In the above-mentioned raw materials, MS is a minimum medium, and cultured tissue grow required inorganic nutritive element and part organotrophy element are provided; 6-benzyl purine 6-BA and 2,4 dichlorophenoxyacetic acid 2,4-D is a plant-growth regulator, has the effect that promotes cultured tissue organizer embryo; Caseinhydrolysate CH provides organic polypeptide class nutritive element; Sucrose provide cultured tissue grow carbon nutrition; The main effect of agar is to solidify substratum, plays a supportive role.
The consumption of above-mentioned each component is that the contriver tests in a large number to grope to sum up on the basis of theory analysis and draws, and prepares substratum according to the above ratio, has and better induces effect.
The making method of above-mentioned substratum is with 6-BA, 2, and 4-D, CH, sucrose, agar are dissolved in MS, stir, and pH value is adjusted between the 5.8-6.0 and gets final product.
Positively effect of the present invention is: utilize the inductivity of basal culture medium garlic somatic embryo can reach 72.0%, one shoot apical meristem and can induce 15 individual embryos.
Embodiment
Further set forth beneficial effect of the present invention by the following examples:
Embodiment 1:
Culture medium prescription:
MS 1L
6-BA 1.0mg
2，4-D 0.1mg
CH 200mg
Sucrose 20g
Agar 6g
Embodiment 2:
Culture medium prescription:
MS 1L
6-BA 1.0mg
2，4-D 1.0mg
CH 200mg
Sucrose 20g
Agar 6g
Embodiment 3:
Culture medium prescription:
MS 1L
6-BA 0.1mg
2，4-D 1.0mg
CH 200mg
Sucrose 20g
Agar 6g
Test method and result:
The substratum of the foregoing description is used for the induction experiment of garlic somatic embryo:
Material: the embryo callus that garlic Xuzhou white garlic shoot apical meristem induces.
Method: the embryo callus tube that garlic Xuzhou white garlic shoot apical meristem is induced is inoculated on the substratum, embryo callus and substratum will be in conjunction with closely, 1 embryo callus of every test tube inoculation, placed 25 ℃, the about 2000Lux of light intensity, illumination every day 12 hours, cultivate 4 week the back statisticses see the following form:
Inducing garlic somatic embryo rate and body embryo quantity (4 week)
Drawn as drawing a conclusion by interpretation of result in the table: the inductivity of body embryo is 36.0% among the embodiment 1, and it is 8.6 that average every callus goes out the embryo number; The inductivity of body embryo is 42.0% in the example 2, and it is 11.5 that average every callus goes out the embryo number; The inductivity of body embryo is 72.0% among the embodiment 3, and it is 15.0 that average every callus goes out the embryo number.
Comprehensive above the analysis, the optimal medium that draws garlic stem tip culture embryo callus body embryonal induction is:
MS 1L
6-BA 0.1mg
2，4-D 1.0mg
CH 200mg
Sucrose 20g
Agar 6g.
Claims (2)
1. substratum that is used for inducing garlic somatic embryo, it is characterized in that, it is with 6-benzyl purine 6-BA, 2,4-dichlorphenoxyacetic acid 2,4-D, caseinhydrolysate CH, sucrose, agar are dissolved among the minimum medium MS, pH value is adjusted at that 5.8-6.0 makes, and the consumption of its each component is: in 1L minimum medium MS add-on:
MS 1L
6-BA 0.1-1.0mg
2，4-D 0.1-1.0mg
CH 200mg
Sucrose 20g
Agar 6g.
2. the substratum that is used for inducing garlic somatic embryo according to claim 1 is characterized in that the consumption of each component is:
MS 1L
6-BA 0.1mg
2，4-D 1.0mg
CH 200mg
Sucrose 20g
Agar 6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100859167A CN100390273C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic somatic embryo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100859167A CN100390273C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic somatic embryo |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1884489A CN1884489A (en) | 2006-12-27 |
| CN100390273C true CN100390273C (en) | 2008-05-28 |
Family
ID=37582796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100859167A Expired - Fee Related CN100390273C (en) | 2006-05-26 | 2006-05-26 | Culture medium for inducing garlic somatic embryo |
Country Status (1)
| Country | Link |
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| CN (1) | CN100390273C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138325B (en) * | 2007-09-20 | 2010-06-09 | 江苏徐淮地区徐州农业科学研究所 | Garlic test tube high-efficiency inducement culture medium |
| CN101396003B (en) * | 2008-08-13 | 2011-03-23 | 山东省农业科学院蔬菜研究所 | Solid culture medium for culturing garlic sexual embryo |
| CN101851601A (en) * | 2010-05-06 | 2010-10-06 | 江苏徐淮地区徐州农业科学研究所 | Culture medium for inducing somatic embryos from garlic embryonic suspension culture system |
| CN111642403B (en) * | 2020-07-24 | 2021-11-26 | 黑龙江省林业科学研究所 | Phellodendron amurense somatic embryo tissue culture medium and tissue culture method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238122A (en) * | 1999-06-15 | 1999-12-15 | 南京农业大学 | Method for quickly multiplying detoxicated garlic seedlings as seed |
| CN1775003A (en) * | 2005-12-15 | 2006-05-24 | 上海交通大学 | Method for producing detoxified sprout by cultivating garlic stem tip combined with cold treatment |
-
2006
- 2006-05-26 CN CNB2006100859167A patent/CN100390273C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238122A (en) * | 1999-06-15 | 1999-12-15 | 南京农业大学 | Method for quickly multiplying detoxicated garlic seedlings as seed |
| CN1775003A (en) * | 2005-12-15 | 2006-05-24 | 上海交通大学 | Method for producing detoxified sprout by cultivating garlic stem tip combined with cold treatment |
Non-Patent Citations (6)
| Title |
|---|
| 大蒜体细胞胚胎发生诱导因素研究. 李步勋等.西北农林科技大学学报（自然科学版），第30卷第5期. 2002 |
| 大蒜体细胞胚胎发生诱导因素研究. 李步勋等.西北农林科技大学学报（自然科学版），第30卷第5期. 2002 * |
| 大蒜发芽叶培养体细胞胚发生. 王洪隆等.华北农学报，第9卷第1期. 1994 |
| 大蒜发芽叶培养体细胞胚发生. 王洪隆等.华北农学报，第9卷第1期. 1994 * |
| 大蒜花梗组织培养再生植株. 王洪隆.华北农学报，第7卷第3期. 1992 |
| 大蒜花梗组织培养再生植株. 王洪隆.华北农学报，第7卷第3期. 1992 * |
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| Publication number | Publication date |
|---|---|
| CN1884489A (en) | 2006-12-27 |
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Granted publication date: 20080528 |