CN103843660A - Culture medium for inducing sweet potato cluster buds - Google Patents
Culture medium for inducing sweet potato cluster buds Download PDFInfo
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- CN103843660A CN103843660A CN201210519917.3A CN201210519917A CN103843660A CN 103843660 A CN103843660 A CN 103843660A CN 201210519917 A CN201210519917 A CN 201210519917A CN 103843660 A CN103843660 A CN 103843660A
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- sweet potato
- culture medium
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- inducing
- agar
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Abstract
The invention relates to a culture medium for inducing sweet potato cluster buds, belonging to a sweet potato tissue culture technology. The culture medium is formed by dissolving kinetin (KT), 6-benzyl purine (6-BA), indoleacetic acid (IAA), casein hydrolysate (CH), cane sugar and agar into a basic culture medium (MS), wherein the dosage of all the components is as follows: 1L of MS, 1.0-2.0mg of KT, 1.0-2.0mg of 6-BA, 0.2-2.0mg of IAA, 200mg of CH, 20g of cane sugar and 6g of agar. The culture medium has the positive effects that after the culture medium is utilized, 8.6 buds can be induced by one stem apical meristem of a sweet potato, so that the induction efficiency is 860%, and the tissue culture efficiency is remarkably improved.
Description
Technical field
The present invention relates to a kind of medium of inducing clumping bud of sweet potato, belong to sweet potato tissue culture technique.Particularly derive the medium of Multiple Buds from the induction of sweet potato stem tip meristematic tissue.
Background technology
The common method of tip Meristem Culture of Sweet Potatoes breeding is that a shoot apical meristem induction differentiates a bud, and this kind method is time-consuming and efficiency is low.In the tissue-culturing rapid propagation of a lot of plants, all utilize the technology that induces Multiple Buds to improve reproduction coefficient, reach the effect of getting twice the result with half the effort.
Summary of the invention
The object of the invention is to overcome the low problem of reproduction coefficient in the cultivation of sweet potato stem tip tissue, provide the medium of the efficient induction of a kind of clumping bud of sweet potato, for the tissue-culturing rapid propagation of sweet potato.
Technical scheme of the present invention is as follows: the medium of this inducing clumping bud of sweet potato is by kinetin KT, 6-benzyl purine 6-BA, heteroauxin IAA, caseinhydrolysate CH, sucrose, agar are dissolved in and form in minimal medium MS, and the consumption of its each component is: in 1L minimal medium MS addition:
MS 1L
KT 1.0-2.0 mg
6-BA 1.0-2.0 mg
IAA 0.2-2.0 mg
CH 200 mg
Sucrose 20 g
Agar 6g
In above-mentioned raw materials, MS is minimal medium, and cultured tissue grow required inorganic nutritive element and part organic nutrition element are provided; Kinetin KT, 6-benzyl purine 6-BA and heteroauxin IAA are plant growth regulator, have the effect that promotes cultured tissue to form Multiple Buds; Caseinhydrolysate CH provides organic many skins class nutritive element; Sucrose provide cultured tissue grow carbon nutrition; The Main Function of agar is solid
Change medium, play a supportive role.The consumption of above-mentioned each component be inventor on the basis of theory analysis, carry out lot of experiments grope sum up draw, prepare according to the above ratio medium, all there is better induction effect.
the preparation method of above-mentioned medium is by KT, 6-BA, and IAA, CH, sucrose, agar are dissolved in MS and stir, and pH value is adjusted between 5.8-6.0.
Good effect of the present invention is: utilize shoot apical meristem of basal culture medium sweet potato can induce 8.6 buds, induction efficiency is 860%, significantly improves group training efficiency.Utilize from a sweet potato stem tip meristematic tissue, induce Multiple Buds, obtain a large amount of homogeneity clones, improve the reproduction coefficient that sweet potato stem tip is cultivated, be easy to batch production and produce sweet potato group training seedling.Swash property, without teratogenesis shape, without sensitization.
embodiment 1
Method: sweet potato Xuzhou 18 shoot apical meristems are inoculated on medium, 1 shoot apical meristem of every test tube inoculation, is placed in 25 DEG C, the about 2000Lux of light intensity, illumination every day 12 hours, cultivate 4 weeks afterwards the inductivity of the visible bud of statistics be 466%, seedling is very thin, fast growth.Comprehensive above analysis, show that the optimal medium of inducing clumping bud of sweet potato is:
MS 1L
KT 1.0mg
6-BA 1.0mg
IAA 0.2 mg
CH 200 mg
Sucrose 20 g
Agar 6g.
Claims (1)
1. a medium for inducing clumping bud of sweet potato, is characterized in that: this medium is by kinetin KT, 6-benzyl purine 6-BA, and heteroauxin IAA, caseinhydrolysate CH, sucrose, agar are dissolved in and form in minimal medium MS, and the consumption of its each component is:
MS 1L
KT 1.0-2.0 mg
6-BA 1.0-2.0 mg
IAA 0.2-2.0 mg
CH 200 mg
Sucrose 20 g
Agar 6g.
2, the medium of inducing clumping bud of sweet potato according to claim 1, is characterized in that: the consumption of medium each component is:
MS 1L
KT 1.0mg
6-BA 1.0mg
IAA 0.2 mg
CH 200 mg
Sucrose 20 g
Agar 6g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210519917.3A CN103843660A (en) | 2012-12-07 | 2012-12-07 | Culture medium for inducing sweet potato cluster buds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210519917.3A CN103843660A (en) | 2012-12-07 | 2012-12-07 | Culture medium for inducing sweet potato cluster buds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103843660A true CN103843660A (en) | 2014-06-11 |
Family
ID=50852119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210519917.3A Pending CN103843660A (en) | 2012-12-07 | 2012-12-07 | Culture medium for inducing sweet potato cluster buds |
Country Status (1)
| Country | Link |
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| CN (1) | CN103843660A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885939A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Ipomoea batatas Lam. |
| CN114431143A (en) * | 2022-02-09 | 2022-05-06 | 廊坊市农林科学院 | Improved MS culture medium and preparation method and application thereof |
-
2012
- 2012-12-07 CN CN201210519917.3A patent/CN103843660A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885939A (en) * | 2015-05-26 | 2015-09-09 | 福建农林大学 | Chemical disinfection tissue culture method for Ipomoea batatas Lam. |
| CN114431143A (en) * | 2022-02-09 | 2022-05-06 | 廊坊市农林科学院 | Improved MS culture medium and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140611 |