CN101103707A - Method for inducing and cultivating tetraploid breeding of sea urchin - Google Patents
Method for inducing and cultivating tetraploid breeding of sea urchin Download PDFInfo
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- CN101103707A CN101103707A CNA2007100124752A CN200710012475A CN101103707A CN 101103707 A CN101103707 A CN 101103707A CN A2007100124752 A CNA2007100124752 A CN A2007100124752A CN 200710012475 A CN200710012475 A CN 200710012475A CN 101103707 A CN101103707 A CN 101103707A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The cultivation method of sea urchin tetraploid fingerlings has the steps that the urchin seedling is dried in shade for 0 to 40 minutes. The 0.5 to 2.0 ml 0.5 mol / L KCl solution is injected from the sea urchin membrana peristomialis for the spawing and spermiation. The urchine eggs and sperms are collected, and the hybrid fertilization is implemented. The developmental temperature of fertilized eggs is controlled between 17 to 18 DEG C. The fertilized eggs in concentration with a mix silk net are collected after 55 to 65 minutes of the fertilization, which are packed in a closed bag and disposed in a pressure vessel of a hydrostatic pressure equipment and the ambient temperature is identified between 17 to 18 DEG C. The pressure treatment is implemented after 60 to 70 minutes of the fertilization, in which the pressure is controlled between 70 to 80MPa and the processing time is controlled between 6 to 8 minutes. Afterwards the fertilized eggs are disposed inside a bin for hatching and stirred, and the incubation temperature is controlled between 16 to 18 DEG C. After floated, the embryos are selected with the proper aeration. The bait feeding is implemented during the larvae cultivation period. The water is exchanged 1 to 3 times everyday, and each time the quantity of water exchange is 1/3 to 2/3. The pond water is exchanged once every three days. The tetraploid fingerlings cultivated with the method can produce the triploid fingerlings, thus the time of processing and the time to the market are extended.
Description
Technical field
The present invention relates to that a kind of sea urchin is tetraploid induces and breeding method.
Background technology
The gonad of sea urchin is nutritious, outside nutrient composition content such as isolating protein and the amino acid height, also contain some special physiological activators such as highly unsaturated fatty acid, have prevention and treatment angiocardiopathy and anticancer function etc., nowadays, the production of sea urchin seedling and the stock enhancement of Japan had become one of the important industry of its " cultivating fishery " already, and France also begins to greatly develop the sea urchin aquaculture, and cultivates good breed kind.China oneself institute introduces after the Strongylocentrotus intermedius, and the artificial breeding and the aquaculture of sea urchin already develop rapidly, has now become a new industry in the northern coastal aquatic product culture industry.
Because of the edible part of sea urchin is a gonad, gonad is subjected to the influence of breeding season bigger, its edible period is shorter, only limit to before and after mating season, the time that causes existing sea urchin can process listing every year is shorter, though therefore existing be that sea urchin seasonal variations in price wild or that culture are very big.Carry out the technical research of sea urchin tetraploid breeding, produce the sea urchin tetraploid, thereby utilize the diploid hybrid of tetraploid and normal sea urchin again, produce full triploid seed, can improve the growth rate and the quality of sea urchin, the time of prolongation sea urchin listing consumption, the economic benefit of raising sea urchin price and breeding enterprise, this also is the prerequisite and the guarantee of lasting, healthy, the stable development of assurance sea urchin aquaculture industry.
Summary of the invention
The purpose of this invention is to provide inducing and breeding method of a kind of tetraploid breeding of sea urchin, and tetraploid is the key of producing the triploid sea urchin that can prolong the sea urchin gonad listing phase, triploid does not breed, therefore has edible sexual gland throughout the year, can prolong the processing Time To Market of sea urchin like this, the sea urchin sexual gland is degenerated and is caused the sea urchin poor quality, can not process and the deficiency of fresh and alive listing period beyond mating season to remedy present sea urchin, increases its nutritive value and economic benefit.
Inducing and breeding method of tetraploid breeding of sea urchin of the present invention comprises the steps:
A, will plant courage and from tank, take out and dry in the shade 0～40 minute, inject the 0.5mol/L KCl solution of 0.5～1.0ml from the sea urchin membrana perisomialis, and wait for and lay eggs and arrange smartly, and collect courage ovum and courage essence respectively, distinguish behind the mixing stand-by with syringe;
B, mix fertilization with the courage ovum of a collection step and courage are smart, after fertilization does not stop to stir, and adopts water-bath, and the developmental temperature of fertilized egg is controlled between 17～18 ℃;
C, after fertilization 55～65 minutes concentrate and collect fertilized egg with the bolting silk net, and the closing bag of packing into places the pressure vessel of hydrostatic pressure equipment, and environmental temperature is defined as between 17～18 ℃;
D, 60～70 minutes of after fertilization beginning pressurized treatments, the pressure in the pressure vessel is controlled between 70～80MPa, the processing time was controlled at 6～8 minutes;
E, fertilized egg is placed in the bucket hatch then, and stir, incubation temperature is controlled between 17～18 ℃, select after the embryo come-up excellent, and an amount of inflation, the bait of throwing something and feeding during the larval culture, change water every day 1～3 time, each quantity of exchanged water is 1/3～2/3, falls pond 1 time in per 3 days, treats that larval metamorphosis is a normal management behind the young sea urchin.
Inducing and breeding method of tetraploid breeding of sea urchin of the present invention wherein saidly will be planted courage to take out the time of drying in the shade from tank be 30 minutes; The consumption of described 0.5mol/L KCl solution is 0.75ml; Described developmental temperature is controlled to be 17.5 ℃; Describedly concentrate and collect fertilized egg at after fertilization row in the time of 60 minutes with the bolting silk net; Described environmental temperature is controlled to be 17.5 ℃; In 65 minutes pressurized treatments of after fertilization, hydrostatic pressure is 75Mpa, and the processing time is 7 minutes; Described incubation temperature is controlled to be 17 ℃; Described bait is Chaetoceros; Change water 2 every day during hatching, each quantity of exchanged water 1/2.
Inducing and breeding method of tetraploid breeding of sea urchin of the present invention, owing to utilized hydrostatic pressure equipment that fertilized egg is handled, there are not problems such as medicament residue and drug contamination in the mode of inducing of this hydrostatic pressure, and induce effective, its essence is and adopt hydrostatic pressing to suppress the spilting of an egg first time of intermediate sea urchin fertilized egg, thereby change the original heredity of fertilized egg and grow the basis, form tetraploid sea urchin seed, the tetraploid sea urchin is to produce the most rapid and simple method of triploid sea urchin, triploid sea urchin gonadal tissue still can be grown, but its sterility and low fertility make it can not normally discharge smart ovum, prolonged the sea urchin gonad listing phase, this can prolong can fresh and alive listing or time of processing supply, thereby remedies the sea urchin market supply in period beyond mating season, and the breed that improves sea urchin is worth.
Embodiment
Inducing and breeding method of tetraploid breeding of sea urchin of the present invention comprises the steps:
A, will plant courage and from tank, take out and dry in the shade 0～40 minute, inject the 0.5mol/L KCl solution of 0.5ml～1ml from the sea urchin membrana perisomialis, and wait for and lay eggs and arrange smartly, and collect courage ovum and courage essence respectively, distinguish behind the mixing stand-by with syringe;
B, mix fertilization with the courage ovum of a collection step and courage are smart, after fertilization does not stop to stir, and adopts water-bath, and the developmental temperature of fertilized egg is controlled between 17～18 ℃;
C, after fertilization 55～65 minutes concentrate and collect fertilized egg with the bolting silk net, and the closing bag of packing into places the pressure vessel of hydrostatic pressure equipment, and environmental temperature is defined as 17～18 ℃;
D, 60～70 minutes of after fertilization beginning pressurized treatments, the pressure in the pressure vessel is controlled at 70～80MPa, the processing time was controlled at 6～8 minutes;
E, fertilized egg is placed in the bucket hatch then, and stir, incubation temperature is controlled at 16～18 ℃, select after the embryo come-up excellent, and an amount of inflation, the Chaetoceros of throwing something and feeding during the larval culture, change water every day 1～3 time, each quantity of exchanged water is 1/3～2/3, falls pond 1 time in per 3 days, treats that larval metamorphosis is a normal management behind the young sea urchin.In implementation process, to above-mentioned said number range, as long as value all can therein.
Described hydrostatic pressure device structure is that a stainless steel pressure jar cylindraceous is arranged, this pressurized tank is made up of the seal between tank body and cover and tank body and the cover, tank inner chamber links to each other with water pump with valve by pipeline, pressure display table is installed on pressurized tank, the water pump water receiving tank, can in jar, pump into seawater, its inner chamber seawater pressure is increased, out of service and pressurize when reaching the pressure of setting.
Claims (2)
1. inducing and breeding method of a tetraploid breeding of sea urchin is characterized in that: comprise the steps:
A, will plant courage and from tank, take out and dry in the shade 0～40 minute, inject the 0.5mol/L KCl solution of 0.5～1.0ml from the sea urchin membrana perisomialis, and wait for and lay eggs and arrange smartly, and collect courage ovum and courage essence respectively, distinguish behind the mixing stand-by with syringe;
B, mix fertilization with the courage ovum of a collection step and courage are smart, after fertilization does not stop to stir, and adopts water-bath, and the developmental temperature of fertilized egg is controlled between 17～18 ℃;
C, after fertilization 55～65 minutes concentrate and collect fertilized egg with the bolting silk net, and the closing bag of packing into places the pressure vessel of hydrostatic pressure equipment, and the water environment temperature is defined as between 17～18 ℃ in the hydrostatic pressure container;
D, 60～70 minutes of after fertilization beginning pressurized treatments, the pressure in the pressure vessel is controlled between 70～80MPa, the processing time was controlled at 6～8 minutes;
E, fertilized egg is placed in the bucket hatch then, and stir, incubation temperature is controlled between 16～18 ℃, select after the embryo come-up excellent, and an amount of inflation, the bait of throwing something and feeding during the larval culture, change water every day 1～3 time, each quantity of exchanged water is 1/3～2/3, falls pond 1 time in per 3 days, treats that larval metamorphosis is a normal management behind the young sea urchin.
2. inducing and breeding method of tetraploid breeding of sea urchin according to claim 1 is characterized in that: describedly will plant courage to take out the time of drying in the shade from tank be 30 minutes; The consumption of described 0.5mol/L KCl solution is 0.75ml; Described developmental temperature is controlled to be 17.5 ℃; Describedly concentrate and collect fertilized egg with the bolting silk net and carried out in 60 minutes at after fertilization; Described environmental temperature is controlled to be 17.5 ℃; In 65 minutes pressurized treatments of after fertilization, pressure is 75MPa, and the processing time is 7 minutes; Described incubation temperature is controlled to be 17 ℃; Described bait is Chaetoceros; Change water 2 every day during hatching, each quantity of exchanged water 1/2.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100124752A CN101103707A (en) | 2007-08-16 | 2007-08-16 | Method for inducing and cultivating tetraploid breeding of sea urchin |
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| CNA2007100124752A CN101103707A (en) | 2007-08-16 | 2007-08-16 | Method for inducing and cultivating tetraploid breeding of sea urchin |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090360A (en) * | 2010-12-17 | 2011-06-15 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102106292A (en) * | 2011-02-10 | 2011-06-29 | 中国水产科学研究院黄海水产研究所 | Flounder triploid batch producing method |
| CN103340164A (en) * | 2013-06-14 | 2013-10-09 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Novel method for inducing Chinese mitten crab triploids |
| CN103960175A (en) * | 2013-01-31 | 2014-08-06 | 中国科学院海洋研究所 | Method for batch induction of scophthalmus maximus tetraploid fries |
| CN105230533A (en) * | 2015-08-04 | 2016-01-13 | 中国水产科学研究院北戴河中心实验站 | Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
| CN112273333A (en) * | 2020-12-15 | 2021-01-29 | 竹溪县隆源大鲵生物开发有限公司 | Artificial breeding insemination frame for giant salamanders |
| CN112273293A (en) * | 2020-10-25 | 2021-01-29 | 马博 | Ovum collecting device with supplementary structure of distinguishing |
| CN112471014A (en) * | 2020-11-25 | 2021-03-12 | 大连海洋大学 | Method for breeding sea urchin seedlings through medium ball of high-density attached plates |
-
2007
- 2007-08-16 CN CNA2007100124752A patent/CN101103707A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090360A (en) * | 2010-12-17 | 2011-06-15 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102090360B (en) * | 2010-12-17 | 2012-02-29 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN102106292A (en) * | 2011-02-10 | 2011-06-29 | 中国水产科学研究院黄海水产研究所 | Flounder triploid batch producing method |
| CN103960175A (en) * | 2013-01-31 | 2014-08-06 | 中国科学院海洋研究所 | Method for batch induction of scophthalmus maximus tetraploid fries |
| CN103960175B (en) * | 2013-01-31 | 2015-09-23 | 中国科学院海洋研究所 | The batch abductive approach of a kind of turbot tetraploid fry |
| CN103340164A (en) * | 2013-06-14 | 2013-10-09 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Novel method for inducing Chinese mitten crab triploids |
| CN105230533A (en) * | 2015-08-04 | 2016-01-13 | 中国水产科学研究院北戴河中心实验站 | Induction and detection method for androgenesis dihaploid of Paralichthys olivaceus |
| CN105230533B (en) * | 2015-08-04 | 2018-07-06 | 中国水产科学研究院北戴河中心实验站 | A kind of induction of lefteye flounder androgenesis dihaploid and detection method |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
| CN105766707B (en) * | 2016-02-01 | 2018-08-24 | 大连海洋大学 | The breeding method of high temperature resistant sea urchin |
| CN112273293A (en) * | 2020-10-25 | 2021-01-29 | 马博 | Ovum collecting device with supplementary structure of distinguishing |
| CN112471014A (en) * | 2020-11-25 | 2021-03-12 | 大连海洋大学 | Method for breeding sea urchin seedlings through medium ball of high-density attached plates |
| CN112273333A (en) * | 2020-12-15 | 2021-01-29 | 竹溪县隆源大鲵生物开发有限公司 | Artificial breeding insemination frame for giant salamanders |
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Open date: 20080116 |