CN101088970B - Process of extracting amino acid for farm use with low value fish - Google Patents
Process of extracting amino acid for farm use with low value fish Download PDFInfo
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- CN101088970B CN101088970B CN200610155516A CN200610155516A CN101088970B CN 101088970 B CN101088970 B CN 101088970B CN 200610155516 A CN200610155516 A CN 200610155516A CN 200610155516 A CN200610155516 A CN 200610155516A CN 101088970 B CN101088970 B CN 101088970B
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 51
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 108091005804 Peptidases Proteins 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000011573 trace mineral Substances 0.000 claims abstract description 7
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 4
- 230000015556 catabolic process Effects 0.000 claims description 17
- 238000006731 degradation reaction Methods 0.000 claims description 17
- 230000000694 effects Effects 0.000 claims description 9
- 229960001484 edetic acid Drugs 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 108010028690 Fish Proteins Proteins 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- 239000002738 chelating agent Substances 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims 3
- 239000000376 reactant Substances 0.000 claims 2
- 239000003337 fertilizer Substances 0.000 abstract description 25
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 3
- 235000010755 mineral Nutrition 0.000 abstract description 3
- 239000011707 mineral Substances 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 7
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 7
- 239000007921 spray Substances 0.000 description 7
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 241000261642 Eupleurogrammus muticus Species 0.000 description 5
- 235000002566 Capsicum Nutrition 0.000 description 4
- 240000008574 Capsicum frutescens Species 0.000 description 4
- 239000001390 capsicum minimum Substances 0.000 description 4
- 239000010813 municipal solid waste Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 3
- 240000003553 Leptospermum scoparium Species 0.000 description 3
- 235000015459 Lycium barbarum Nutrition 0.000 description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 229960002645 boric acid Drugs 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 3
- 239000011790 ferrous sulphate Substances 0.000 description 3
- 235000003891 ferrous sulphate Nutrition 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 239000011572 manganese Substances 0.000 description 3
- 229940099596 manganese sulfate Drugs 0.000 description 3
- 239000011702 manganese sulphate Substances 0.000 description 3
- 235000007079 manganese sulphate Nutrition 0.000 description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 description 3
- 229960001763 zinc sulfate Drugs 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 229910052796 boron Inorganic materials 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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Abstract
The technological process of producing amino acid fertilizer for farm use with low value fish includes adding protease into low value fish to degrade at 40-60 deg.c and pH 6-8, in the solid/liquid ratio of 1 to 6-8 and in the presence of EDTA for 4 hr to obtain degraded liquid; adding proper amount of trace elements and regulating pH value to 6.5. The present invention has reasonable process, can produce liquid amino acid fertilizer with the low value fish as waste, recover rich protein and minerals from low value fish and utilize resource effectively.
Description
Technical field
The invention belongs to chemical field, relating to a kind of is raw material with the low value fish, through enzymolysis and chelating technology, extracts the technology of agricultural amino acid.
Background technology
In recent years, along with the development of fishery and fish processing industry, produced a large amount of low value fish, these low value fishes not only contain amounts of protein, also contain multiple bioactivator.It is reported that blue or green squama fish offal material crude protein content can reach 58.7% (butt), crude protein content is up to 13.5% (wet basis) in the eel head, and protein content is 13.52% in the bighead leftover bits and pieces.And in fact these resources are not reasonably utilized.Though the existing amino acid whose technology of aquatic products degraded extraction of utilizing has a large amount of mineral matters precipitated discarded, and produces a large amount of organic wastewater, has brought pollution to environment.
Summary of the invention
The technology that the purpose of this invention is to provide a kind of extracting amino acid for farm use with low value fish.
What the present invention will solve is the problem that the discarded object low value fish resource in existing fishery and the fish processing industry is not rationally utilized.
Work step of the present invention is suddenly:
(1) the low value fish is cleaned up making beating;
(2) enzyme degraded: 40～60 ℃ of temperature, under the condition of pH value 6～8, add protease, protease is measured 2～9% of low value fish body internal protein content, is 1: 6～8 solid-to-liquid ratio ratio with the ratio of solid low value fish and water quality, in the presence of chelating agent ethylenediamine tetra-acetic acid (EDTA), degraded 4 hours, low value fish degradation rate is 85～95% degradation solution, after the degraded, the amino acid productive rate of low value fish protein is 6～10%;
(3) in the degradation solution, amino acid content accounts for 25.6～35% of total amount, and trace element accounts for about 0.9%, and degradation solution pH is alkalescence.
The present invention adopts enzymolysis and chelating technology combination, and more nutrition is retained in the extract, adds an amount of trace element and regulate the pH value about 6.5 in degradation solution of the present invention, just can be used as the fertile use of amino acid liquid.So the present invention not only makes discarded object low value fish resource rationally be utilized, and technology is simple, nutrient is comprehensive, and is agricultural effective.
Description of drawings
Fig. 1 is a process flow diagram of the present invention.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment one
(1) choose low value trash fishes such as eupleurogrammus muticus, clean up making beating;
(2) enzyme degraded: in temperature is 50 ℃, pH is about 6.5, add protease, the unit of activity 30000u/g of protease, protease is measured 8% of low value fish body internal protein content such as eupleurogrammus muticus, is 1: 7 solid-to-liquid ratio ratio with the ratio of solid low value fish and water quality, in the presence of chelating agent ethylenediamine tetra-acetic acid (EDTA), reacted 4 hours, obtain trash fishes such as eupleurogrammus muticus degradation solution; The degradation rate of trash fishes such as eupleurogrammus muticus is 92.53%.After the degraded, the amino acid productive rate of trash fish protein such as eupleurogrammus muticus is 8.99%.
(3) amino acid accounts for 26.3% of degradation solution total amount in this degradation solution, and trace element accounts for 0.98%, and degradation solution pH is alkalescence.
Add trace element (Fe, Mn, Cu, Zn, B) in the above-mentioned degradation solution and reach 2%, and regulate the pH value about 6.5, just can be used as the fertile use of amino acid liquid.Trace element replenishes and finishes by interpolation ferrous sulfate, copper sulphate, zinc sulfate, manganese sulfate, boric acid.
The liquid fertilizer of this seed amino acid is rich in 17 seed amino acids, polysaccharide and Cu, Fe, Mn, the various nutriments such as B, Zn that are beneficial to growth and development of plants.Can foliage-spray, soil application, seed soak.Wherein free amino acid and mineral mass-energy are utilized by plant absorbing rapidly; Polypeptide and polysaccharide discharge lentamente by normal biological activity in soil, satisfy the needs of various crops.Can also make edaphon double to breed, improve activity, activating soil, promotion crop are absorbed nutrient, adjusting soil acidity or alkalinity etc. effect unique.To the melon and fruit of cultivation, the vegetables effect is more obvious, mainly shows: crop root prosperity, sturdy, the premunition increase of cane, and output is increased substantially, quality is significantly improved.
The effect of amino acid liquid fertilizer describes in conjunction with following test effect.
Test 1: amino acid liquid fertilizer (No. 1, fish albumen liquid fertilizer, No. 2, fish albumen liquid fertilizer) is to the influence (is contrast with the clear water) of crop growth.
No. 1, fish albumen liquid fertilizer: added ferrous sulfate, copper sulphate, zinc sulfate, manganese sulfate, boric acid in the degradation solution.
No. 2, fish albumen liquid fertilizer: added ferrous sulfate, copper sulphate, zinc sulfate, manganese sulfate, boric acid and algae essence in the degradation solution.
Respectively corresponding test is done in Chinese cabbage growth of seedling, tea tree germination, tomato nutrient growth No. 2 with No. 1, fish albumen liquid fertilizer and fish albumen liquid fertilizer, is drawn as drawing a conclusion:
1), with No. 1, No. 2 seed soaking of fish albumen liquid fertilizer, all can improve yield of pakchoi and increase plant height.Contrast volume increase 36.68% and 27.46% respectively, plant height contrasts respectively increases by 16.78% and 21.67%.
2), spray tea tree No. 1, No. 2 with fish albumen liquid fertilizer, can promote tea tree to sprout, bud-leaf is sturdy, and bud head density increases, and it is strong to hold tender property, plucks, and has improved early yield in 3～5 days in advance.
3), spray tomato No. 1, No. 2, can promote the absorption and accumulation of plant, and comparison is according to improving 73.77% and 65.89% respectively to the nitrogen element with fish albumen liquid fertilizer.
Test 2: amino acid liquid fertilizer (No. 1, fish albumen liquid fertilizer, No. 2, fish albumen liquid fertilizer) is to yield of vegetables and quality influence.
With fish albumen liquid fertilizer No. 1, No. 2, respectively Chinese cabbage, capsicum are done corresponding test, draw as drawing a conclusion:
1), behind the foliage-spray fish albumen liquid fertilizer, the pakchoi growth is vigorous, the leaf look dark green, and improved output, No. 1, No. 2 contrast volume increase 28.8% and 27.0% respectively of liquid fertilizer, and fish albumen liquid fertilizer also can improve the content of Vc in the Chinese cabbage.
2), the output, the Vc content that spray capsicum of fish albumen liquid fertilizer all has significant facilitation.Make capsicum contrast volume increase 55.94% and 32.88% respectively, the content of Vc also contrasts respectively and has improved 30.02% and 19.20%.Simultaneously, also significantly improve the absorption of capsicum to Ca, Mg, Fe, Mn, Cu, Zn.
Test 3: amino acid liquid fertilizer is used a dual standard test to the anti-influence of coercing ability of Chinese cabbage and foliage-spray and ground.
The result as can be seen, use fish albumen liquid fertilizer No. 1, No. 2 after, can alleviate the lipid peroxidation of crop in the adverse circumstance environment, improve resistance.Mda content is compared respectively according to reducing by 24.28% and 12.99% in the Chinese cabbage body.From same effect also being arranged to organizing the permeability aspect to see.After using fish albumen liquid fertilizer, the fabric texture permeability of handling through adverse circumstance of doing is had the reduction effect, wherein foliage-spray can be compared according to reducing about 37%.
Claims (2)
1. the technology of an extracting amino acid for farm use with low value fish, the concrete implementation step of this technology is:
(1) the low value fish is cleaned up making beating, get the fish slurries; And the solid-to-liquid ratio of dilute with water is 1: 6～8 fish slurry reactant liquor;
(2) enzyme degraded: 40～60 ℃ of temperature, under the condition of pH value 6～8, add protease at fish slurry reactant liquor, the unit of activity 30000-300000u/g of protease, protease is measured 2～9% of low value fish body internal protein content, in existence of chelating agent ethylenediamine tetra-acetic acid and ethylenediamine tetra-acetic acid consumption is under the 1-5% condition of low value fish quality, degrades 4 hours, obtains degradation solution; Amino acid productive rate after the degraded of low value fish protein is 6～10%;
(3) in degradation solution, amino acid content accounts for 25.6～35% of low value fish degradation solution total amount, and trace element accounts for about 0.9%, and degradation solution pH is alkalescence.
2. the technology of extracting amino acid for farm use with low value fish according to claim 1 is characterized in that solid-to-liquid ratio is a solid low value fish and the ratio of water quality.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200610155516A CN101088970B (en) | 2006-12-27 | 2006-12-27 | Process of extracting amino acid for farm use with low value fish |
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| CN200610155516A CN101088970B (en) | 2006-12-27 | 2006-12-27 | Process of extracting amino acid for farm use with low value fish |
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| CN101088970A CN101088970A (en) | 2007-12-19 |
| CN101088970B true CN101088970B (en) | 2010-05-26 |
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Families Citing this family (18)
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| CN102060600B (en) * | 2010-10-11 | 2013-01-23 | 山东郁茏生物科技有限公司 | Fish protein organic compound fertilizer and preparation method thereof |
| CN103058716A (en) * | 2013-01-14 | 2013-04-24 | 广西穗宁化工集团有限公司 | Organic matter liquid suitable for being used as leaf fertilizer raw material |
| CN103553749B (en) * | 2013-11-11 | 2015-01-14 | 李世泰 | Method for producing amino acid agricultural fertilizer through compound enzyme hydrolysis of fish skins and fish scales |
| CN104803731A (en) * | 2015-04-20 | 2015-07-29 | 青岛诺海生物科技有限公司 | Method for preparing ocean composite peptide biological fertilizer through solid state fermentation |
| CN104892037B (en) * | 2015-06-10 | 2016-06-08 | 荣成鸿德海洋生物科技有限公司 | A kind of take fish as the method for the water-soluble fish-protein organic fertilizer of raw material production |
| CN105254344A (en) * | 2015-10-29 | 2016-01-20 | 广东省农业科学院蚕业与农产品加工研究所 | Method for preparing liquid amino acid liquid fertilizer from tilapia mossambica processed wastes, liquid amino acid liquid fertilizer and application of liquid amino acid liquid fertilizer |
| CN105254345A (en) * | 2015-11-20 | 2016-01-20 | 山东颐阳生物科技有限公司 | Preparation method of amino acid liquid fertilizer for American ginseng |
| CN105618158A (en) * | 2015-12-19 | 2016-06-01 | 仇颖超 | Method for preparing amino acid hydrotalcite-like catalyst carrier |
| CN106478271A (en) * | 2016-11-10 | 2017-03-08 | 云南农业大学 | A kind of water-soluble fertilizer of aminoacid containing enzymatic isolation method and preparation method thereof |
| CN106631558A (en) * | 2016-11-15 | 2017-05-10 | 陈景河 | Ecological environmental protection fish protein liquid fertilizer |
| CN108218611A (en) * | 2016-12-22 | 2018-06-29 | 北京天缘生物科技有限公司 | A kind of novel plant nutritional agents and preparation method thereof |
| CN106747809A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method that utilization processing of aquatic products accessory substance prepares liquid protein fertilizer |
| CN106748150A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A kind of method of low value ocean fish fermenting and producing amino acid foliage fertilizer |
| CN106748190B (en) * | 2016-12-28 | 2018-03-20 | 烟台泓源生物肥料有限公司 | One kind fermentation Optimization of Low Value Fish water-soluble fertilizer and preparation method thereof |
| CN111111255B (en) * | 2019-11-14 | 2021-09-17 | 南开大学 | Method for extracting amino acid from soybean phloem |
| CN111875434B (en) * | 2020-07-27 | 2022-02-01 | 青岛农业大学 | Cold-resistant and vernalization-promoting tea tree liquid fertilizer and application thereof |
| CN114085101A (en) * | 2021-12-09 | 2022-02-25 | 山东省神农生态科技股份有限公司 | Production method of fish protein fertilizer and fish protein fertilizer prepared by same |
| CN117209314A (en) * | 2023-09-15 | 2023-12-12 | 广州优卡思农业技术有限公司 | Fish protein organic liquid fertilizer and production process thereof |
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- 2006-12-27 CN CN200610155516A patent/CN101088970B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005198558A (en) * | 2004-01-15 | 2005-07-28 | Sanshii Foods:Kk | Amino acid-containing food raw material and method for producing the same |
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| 郑伟，石伟勇.利用低值鱼制备鱼蛋白液肥及其生物学效应的研究.浙江大学,2006,摘要，17，22-27. * |
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