CN101020703A - Total alkaloid of pink plumepoppy herb - Google Patents
Total alkaloid of pink plumepoppy herb Download PDFInfo
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- CN101020703A CN101020703A CN 200610051261 CN200610051261A CN101020703A CN 101020703 A CN101020703 A CN 101020703A CN 200610051261 CN200610051261 CN 200610051261 CN 200610051261 A CN200610051261 A CN 200610051261A CN 101020703 A CN101020703 A CN 101020703A
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Abstract
The present invention discloses total alkaloid of pink plumepoppy herb used as material for green medicine, veterinary medicine and biological pesticide. The total alkaloid of pink plumepoppy herb is prepared with the effective component extracted from pink plumepoppy herb. The present invention has the features of innovative technological process, wide application range in medicine, veterinary medicine and biological pesticide, no toxic side effect, high bioavailability, etc.
Description
The present invention relates to a kind of really is fully natural green medicine, veterinary drug, the biological pesticide raw material that raw material compatibility behind the extracting effective components total alkaloids is made with the plant Pink Plumepoppy Herb, belongs to the biological medical product field.
The objective of the invention is to: a kind of bioactive ingredients that derives from the natural phant Pink Plumepoppy Herb fruit is provided, through processes such as extraction separation purifying drying refining form have antisepsis and anti-inflammation, suppress multiple pathogenic bacteria, clearing heat and detoxicating, anti-kinds of tumors; Desinsection, sterilization are widely used in vegetables, fruit, cotton, the multiple bioactive medicine such as the prevention and control of plant diseases, pest control of food crop, veterinary drug, biological pesticide raw material macleaya cordata total alkaloid, are a kind of fully natural green biomedical products.It can be as the raw material of producing injection liquid, oral liquid, tablet, capsule, granule and biological pesticide wettable powder, solution.Do not produce any toxic side effects after its uses, noresidue, easy to use, evident in efficacy, steady quality, applied range; Meet the medication requirement of producing non-polluted farm product, green agricultural product, to overcome the deficiency of existing medicine.
The present invention be achieved in that it by Pink Plumepoppy Herb fruit through the extraction of ethanol step impregnation, ultrasonication, vacuum concentration filter step by step, refining the forming of production technique of the removal of impurities of cold and hot processing precipitation, hybrid combination, macroporous resin separation and purification, microwave vacuum drying.
1. get Pink Plumepoppy Herb and really make meal, with 75%, 85%, 95% ethanol 50 ℃ of-60 ℃ of each ultrasonication lixiviates once (the ethanol consumption be the medicinal material amount 3-5 doubly, ultrasonic resonant frequency 22-26kHz), each 1-2 hour.Extract back merging alcohol extract, filter, decompression recycling ethanol to extracting solution contains the alcohol amount to be ended when 30%-40%, filtered while hot, and filtrate 0.1%-0.3% adding activated carbon by volume is heated with stirring to and boils, and filters de-carbon.12-24 hour after-filtration left standstill at cold place, and filtration is left standstill in refrigeration after adding equivalent distilled water and transferring pH3-5, and the percentage concentration requirement heating back distilled water constant volume by certain adds the 1%-2% tween, gets the total alkaloids extracting solution, measures total alkaloid content, refrigerates standby.
2. it is an amount of to take by weighing respectively the NKA-9 type macroporous absorbent resin of having handled well; Wet method is packed in the chromatographic column of 2-4cm * 300-600cm; (upper prop TA amount and resin ratio are as 1: 10-15 take the flow velocity upper prop of 2mL-3mL/min with macleaya cordata fruit extract; Upper prop liquid TA concentration is 10-15mg/mL; Upper prop liquid pH value 3～5); Cross the complete rear distilled water with 2-4 times of resin volume of post and wash post to going out water colorless with the speed of 3mL-4mL/min; Be eluted to water white transparency with the ethanol of 2-3 times of resin volume 80-90% with the elution speed of 2-3mL/min again
3. collection ethanol eluate, concentrating under reduced pressure, microwave vacuum drying was pulverized the 80-120 mesh sieve, weighed, and measured total alkaloid content and should be not less than 95%.
4. the present invention compared with prior art has following advantage:
4.1 process innovation.Technical process science of the present invention is succinct, and whole production technology has been taked refining the forming of production technique that the extraction of ethanol step impregnation, ultrasonication, vacuum concentration filter step by step, cold and hot processing precipitates removal of impurities, hybrid combination, macroporous resin separation and purification, microwave vacuum drying.Finings and activated carbon removal of impurities in technology, have been used.Its finished product active constituent content height, steady quality, color and luster degree are good.Be suitable for suitability for industrialized production, packing of product transportation is easy to carry.
4.2 the present invention is fully natural green medicine, veterinary drug, biological pesticide raw produce, uses the secondary residual action of the no any poison in back, and is safe and reliable, easy to use, applied range is evident in efficacy, instant effect; Meet the medication requirement of producing non-polluted farm product, green agricultural product, globality, pleiotropy, amphicheirality, synergitic traditional compatibility advantage that herbal medicine is cured the disease and regulated have promptly been brought into play, modern science and technology have been embodied again to the refining advantage of herbal medicine, advantage such as easy to use, quick, portable.Meet " green product " developing direction.
4.3 applied range.Macleaya cordata total alkaloid is a kind of antisepsis and anti-inflammation that has, and suppresses multiple pathogenic bacteria, and is clearing heat and detoxicating, anti-kinds of tumors; Desinsection, sterilization are widely used in multiple bioactive medicine, veterinary drug, the biological pesticide raw materials such as the prevention and control of plant diseases, pest control of vegetables, fruit, cotton, food crop, are a kind of fully natural green biology " three medicines " raw produce.It can be as the raw material of producing injection liquid, oral liquid, tablet, capsule, granule and biological pesticide wettable powder, solution.
Embodiments of the invention:
1. get Pink Plumepoppy Herb and really make meal 5000g, get 75%, 85%, 95% ethanol 50 ℃ of-60 ℃ of each ultrasonication lixiviates once (the ethanol consumption be the medicinal material amount 3-5 doubly, ultrasonic resonator frequency 22-26kHz), each 1 hour.Extract back merging alcohol extract, filter, decompression recycling ethanol to extracting solution contains the alcohol amount to be ended at 30% o'clock, filtered while hot, and filtrate 0.1% adding activated carbon stirring heating is by volume filtered de-carbon.12 hours after-filtration are left standstill at cold place, leave standstill 12 hours after-filtration in 2～4 ℃ of refrigerations after adding equivalent distilled water and transferring pH3-5, the total alkaloids concentration requirement distilled water constant volume of 1.6-2% is pressed in the heating back, add 2% tween, get total alkaloids extracting solution (total alkaloid content: 15mg/mL), refrigerate standby.
2. take by weighing the NKA-9 type polymeric adsorbent 500g that has handled well respectively, wet method is packed in the chromatographic column of 3cm * 500cm, (upper prop total alkaloids amount is 1: 12 with the resin ratio with the flow velocity upper prop of 2mL-3mL/min with the total alkaloids extracting solution, upper prop liquid total alkaloids concentration is 15mg/mL, upper prop liquid pH value 4-5), cross the intact back of post and wash post to going out water colorless with the speed of 3mL-4mL/min, be eluted to water white transparency with the ethanol of 2-3 times of resin volume 85% with the elution speed of 2-3mL/min again with the distilled water of 2-3 times of resin volume.
3. collect 85% ethanol eluate, be evaporated to the medicinal extract shape, microwave vacuum drying was pulverized 100 mesh sieves, weighed, and measured total alkaloid content, and finished product contains the effective ingredient total alkaloids more than 95%, can, sealing, quality inspection, packing.
Claims (2)
1. a pure natural medicine, veterinary drug, biological pesticide raw material, macleaya cordata total alkaloid.It really is a raw material with the plant Pink Plumepoppy Herb, refining forming after the production technique that ethanol step impregnation, ultrasonication are extracted, vacuum concentration filters step by step, cold and hot processing precipitates removal of impurities, hybrid combination, macroporous adsorbent resin separation and purification, microwave vacuum drying is extracted its effective constituent total alkaloids is characterized in that:
Really make meal 1.1 get Pink Plumepoppy Herb, with 75%, 85%, 95% ethanol 50 ℃ of-60 ℃ of each ultrasonication lixiviates once (the ethanol consumption be the medicinal material amount 3-5 doubly, ultrasonic resonator frequency 22-26kHz), each 1-2 hour.Extract the back and merge the alcohol extract filtration, decompression recycling ethanol to extracting solution contains the alcohol amount to be ended when 30%-40%, filtered while hot, and filtrate 0.1%-0.3% by volume adds the activated carbon stirring heating, the filtration de-carbon.12-24 hour after-filtration left standstill at cold place, and filtration is left standstill in refrigeration after adding equivalent distilled water and transferring pH3-5, and the percentage concentration requirement heating back distilled water constant volume by certain adds the 1%-2% tween, gets the total alkaloids extracting solution, measures total alkaloid content, refrigerates standby.
1.2 it is an amount of to take by weighing the NKA-9 type macroporous adsorbent resin of having handled well, wet method is packed in the chromatographic column of 2-4cm * 300-600cm, (upper prop liquid total alkaloids amount and resin are than by 1: 10-15 with the flow velocity upper prop of 2mL-3mL/min with Pink Plumepoppy Herb fruit extracting solution, upper prop liquid total alkaloids concentration is 10-15mg/mL, upper prop liquid pH value 3～5), cross the intact back of post and wash post to going out water colorless with the speed of 3mL-4mL/min, be eluted to water white transparency with the ethanol of 2-3 times of resin volume 80%-90% with the elution speed of 2-4mL/min again with the distilled water of 2-4 times of resin volume.
1.3 the collection ethanol eluate, concentrating under reduced pressure, microwave vacuum drying was pulverized the 80-120 mesh sieve, weighed, and measured total alkaloid content and should be not less than 95%.
2. macleaya cordata total alkaloid can be as the raw material of producing injection liquid, oral liquid, tablet, capsule, granule and biological pesticide wettable powder, solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610051261 CN101020703A (en) | 2006-11-01 | 2006-11-01 | Total alkaloid of pink plumepoppy herb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610051261 CN101020703A (en) | 2006-11-01 | 2006-11-01 | Total alkaloid of pink plumepoppy herb |
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| Publication Number | Publication Date |
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| CN101020703A true CN101020703A (en) | 2007-08-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200610051261 Pending CN101020703A (en) | 2006-11-01 | 2006-11-01 | Total alkaloid of pink plumepoppy herb |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258580A (en) * | 2011-07-01 | 2011-11-30 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloid preparation method |
| CN102318648A (en) * | 2011-07-01 | 2012-01-18 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids flea-killing spraying agent |
| CN102318601A (en) * | 2011-07-01 | 2012-01-18 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids water dispersion granule disinfectant and preparation method thereof |
| CN101347512B (en) * | 2008-09-05 | 2012-02-22 | 郑州后羿制药有限公司 | Macleaya cordata injection and preparation thereof |
| CN102578164A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic pesticide containing sophocarpidine and macleaya alkaloids and preparation method of compounded botanical synergistic pesticide |
| CN104013559A (en) * | 2014-06-28 | 2014-09-03 | 管天球 | Anti-scurf shampoo containing tea saponin |
| CN104996401A (en) * | 2015-08-12 | 2015-10-28 | 贵阳学院 | Biopesticide emulsifiable concentrate preparation for controlling vegetable pest small white |
| CN109369729A (en) * | 2018-09-06 | 2019-02-22 | 铜仁职业技术学院 | A kind of method that inner ebullition method extracts Macleaya cordata alkaloids |
| CN113332340A (en) * | 2021-06-01 | 2021-09-03 | 铜仁职业技术学院 | Plant compound extract and application thereof in preparation of macleaya cordata oral liquid |
-
2006
- 2006-11-01 CN CN 200610051261 patent/CN101020703A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347512B (en) * | 2008-09-05 | 2012-02-22 | 郑州后羿制药有限公司 | Macleaya cordata injection and preparation thereof |
| CN102318601B (en) * | 2011-07-01 | 2013-08-07 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids water dispersion granule disinfectant and preparation method thereof |
| CN102318601A (en) * | 2011-07-01 | 2012-01-18 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids water dispersion granule disinfectant and preparation method thereof |
| CN102318648A (en) * | 2011-07-01 | 2012-01-18 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids flea-killing spraying agent |
| CN102258580B (en) * | 2011-07-01 | 2013-04-24 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloid preparation method |
| CN102258580A (en) * | 2011-07-01 | 2011-11-30 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloid preparation method |
| CN102318648B (en) * | 2011-07-01 | 2013-11-06 | 湖南汉清生物技术有限公司 | Macleaya cordata total alkaloids flea-killing spraying agent |
| CN102578164A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic pesticide containing sophocarpidine and macleaya alkaloids and preparation method of compounded botanical synergistic pesticide |
| CN102578164B (en) * | 2012-01-20 | 2013-11-27 | 山西德威生化有限责任公司 | Compounded botanical synergistic pesticide containing sophocarpidine and macleaya alkaloids and preparation method of compounded botanical synergistic pesticide |
| CN104013559A (en) * | 2014-06-28 | 2014-09-03 | 管天球 | Anti-scurf shampoo containing tea saponin |
| CN104996401A (en) * | 2015-08-12 | 2015-10-28 | 贵阳学院 | Biopesticide emulsifiable concentrate preparation for controlling vegetable pest small white |
| CN109369729A (en) * | 2018-09-06 | 2019-02-22 | 铜仁职业技术学院 | A kind of method that inner ebullition method extracts Macleaya cordata alkaloids |
| CN113332340A (en) * | 2021-06-01 | 2021-09-03 | 铜仁职业技术学院 | Plant compound extract and application thereof in preparation of macleaya cordata oral liquid |
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Open date: 20070822 |