CN100536658C - Fume smoking sanitizer for sericultural production and preparing method thereof - Google Patents
Fume smoking sanitizer for sericultural production and preparing method thereof Download PDFInfo
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- CN100536658C CN100536658C CNB2007100709569A CN200710070956A CN100536658C CN 100536658 C CN100536658 C CN 100536658C CN B2007100709569 A CNB2007100709569 A CN B2007100709569A CN 200710070956 A CN200710070956 A CN 200710070956A CN 100536658 C CN100536658 C CN 100536658C
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Abstract
The present invention discloses a fumigation agent and the preparation method in sericulture industry belonging to the technical field of disinfecting medicament. The prescription of the fumigation agent (weight) is that 25 to 60 shares of trichloroisocyanuric acid, 3 to 10 shares of chlorothalonil, 6 to 18 shares of combustion promoter and 7 to 20 shares of fire retardant. With the sealing fumigation of 5g/m<SUP>3</SUP> dosage for 5 hours, the disease causes of silkworms can be removed; with fumigation of 0.5g/m<SUP>3</SUP> trichloroisocyanuric acid for 30 minutes in rearing season, the prevention and cure effect of white muscardine and aspergillus is better than the anti-disease No.1 and is almost the same as that of fumigation with 1g/m<SUP>3</SUP> trichloroisocyanuric acid; the cost is reduced by about 50 percent; at the same time, the present invention is convenient for use and labor-saving, which is used in the grown silkworm stage with more than 80 percent of labor being reduced; the package of the product is rational and the shelf life is as long as 2 years; the storage and transportation of the product are safe and the product can be used for disinfecting medicament of tools in rearing room or rearing bed of silkworm body.
Description
Technical field
The invention belongs to technical field of disinfecting medicament, especially belong to sootiness disinfectant and preparing technical field thereof that silkworm already produces.
Background technology
Prevention silkworm disease must be done the silkworm room disinfection of tools for silkworm rearing in early stage and the silkworm body and seat disinfection in the feeding process well, wherein, the silkworm room rearing instrument mainly adopts efficient medicament such as bleaching powder extraction to carry out disinfection, and silkworm body and seat mainly adopts the safer diseases prevention of silkworm is carried out disinfection for No. one.This medicine is a host with formaldehyde, has characteristics efficient, wide spectrum, tool solid phase and gas phase double sterilization effect, though promote nearly 30 years, but still lasting, be the first-use drug of silkworm body and seat sterilization in the current production.But this medicine is a pulvis, the serious stimulation applications person of dust during use, and labour intensity is very big, and its active ingredient formaldehyde has the anxiety of " three cause " harm; In addition, along with the pattern of rural area sericulture in recent years changes, sericulture is by the highly intensive labour of past Hui Yu more than a day, and is excessive to laborsavingization modes such as the rearing with less number of feeds a day on the one, the raising of bar mulberry cutworm, booth shoot rearings, so the sterilization of silkworm body and seat also requires to adapt to the needs of Laborsaving Rearing.Silkworm industry worker is used for disinfection on silkworm rearing with fumigating disinfectant, and tool is laborsaving, the timesaving characteristics.Produce the fumigating disinfectant of going up implementation at present formaldehyde fumigant, DCCNa fumigant, sulphur, TCCA (Trichloroisocyanuric acid) fumigant etc. are arranged, these medicines respectively have characteristics, but also have weak point, use trouble as formaldehyde fumigant, effect is better when requiring room temperature to reach more than 25 ℃ during use, it is improper to grasp as furnace, and polyformaldehyde can volatilize not exclusively, or catching fire, and in disinfecting process, because of flue gas come-up, in the silkworm room in, the upper strata Disinfection Effect is better, and ground and lower floor's Disinfection Effect are relatively poor; The DCCNa fumigant is easy to use, but also has flue gas come-up, in, the upper strata Disinfection Effect is good, and ground and the relatively poor shortcoming of lower floor's Disinfection Effect, as disinfection on silkworm rearing, the silkworm body and seat that cutworm is educated does not reach the sterilization requirement; Sulphur is only effective to fungal spore, and uses trouble; Though the TCCA (Trichloroisocyanuric acid) fumigant is easy to use, sootiness performance and drug effect are all good, but this product is because its host and assistant agent are respectively Oxidizing and Reducing Agents, hybrid packed together after the moisture absorption very easily, both dangerous in storage, transportation, unstable again, available chlorine descends very fast, and the shelf-life is only about half a year; In addition, though find also that in actual use this fumigant is pretty good to the fungal disease control efficiency, its dosage is bigger, and use cost is higher.
Summary of the invention
The present invention seeks to, at No. one, above-mentioned silkworm body and seat disinfectant diseases prevention and the existing defective of TCCA (Trichloroisocyanuric acid) fumigant, propose a kind of have sterilization spectrum wide, efficient, to silkworm safety, can make disinfectant for silkworm room and ware, can make the silkworm body and seat disinfectant again, easy to use, laborsaving, saving of labor, cost is lower, and smog is evenly distributed, the packing of product is reasonable, and the silkworm that shelf life of products is long is used fumigating disinfectant; Another object of the present invention is the preparation method who proposes this fumigating disinfectant.
The object of the invention is achieved by the following technical programs.
A kind ofly be used for the fumigating disinfectant that silkworm already produces, the component of this fumigating disinfectant and the weight portion of each component are:
25～60 parts of TCCA (Trichloroisocyanuric acid)
3～10 parts of tpns
6～18 parts of combustion adjuvants
7～20 parts of fire retardant chemicals.
The optimization of C of described fumigating disinfectant, the weight portion of its component and each component is: 45 parts of TCCA (Trichloroisocyanuric acid), 6.5 parts of (90%) tpns, 10.5 parts in combustion adjuvant flour, 12 parts in fire retardant chemical potter's clay powder.
Described combustion adjuvant is wood dust, flour or carbon dust.
Described fire retardant chemical is talcum powder, potter's clay powder or zeolite powder.
A kind ofly be used for the preparation method that silkworm already produces fumigating disinfectant, carry out according to the following steps:
(1) after tpn, combustion adjuvant and fire retardant chemical being crossed 24 mesh sieves respectively, weighing, by formula rate drop into mix in the Multidimensionblender after, weigh, be packaged into parcel with packing inside bag;
(2) TCCA (Trichloroisocyanuric acid) is crossed 24 mesh sieves, weighed, be packaged into big bag with packing inside bag;
(3) with the parcel of step (1) and the big bag of step (2), place in the outer packaging bag, seal, check, warehouse-in or transportation and sales disinfectant products.
Beneficial effect of the present invention:
The one, this strain is a host with high chlorinated product TCCA (Trichloroisocyanuric acid) and disinfectant use in agriculture tpn, and formulated compound TCCA (Trichloroisocyanuric acid) fumigant is used 5g/M
3Airtight smoked the disappearing 5 hours of dosage, all can kill the various cause of diseases such as bacterium, fungi, NPB, CPB, N.b and particulate of silkworm, drug effect and TCCA (Trichloroisocyanuric acid) fumigant are similar, so can be used for the sterilization of airtight silkworm room, rearing instrument, bunch chamber and the chamber of hastening the hatching of silkworms.
The 2nd, silkworm rearing season 0.5g/m
3Compound TCCA (Trichloroisocyanuric acid) fumigant is smoked to disappear 30 minutes, and the stiff and aspergillus control efficiency of dialogue is better than diseases prevention No. one, and with consumption be 1g/m
3TCCA (Trichloroisocyanuric acid) fumigant effect suitable, thereby obviously improved control efficiency to the fungal disease of silkworm rearing season silkworm body and seat; Test is also clear and definite, and this medicament uses in silkworm rearing season common species, original seed safety all, and silkworm physiology is not produced harmful effect yet.
The 3rd, when this fumigant was fuming smoked disappearing, flue gas upper, middle and lower, ground, left, center, right in airtight silkworm room were evenly distributed, and the Disinfection Effect unanimity has overcome general fumigant causes lower floor and ground sterilization poor effect because of the flue gas come-up shortcoming; This product is smoked when disappearing simultaneously, and is easy to use, saving of labor, laborsaving, and big silkworm rearing season uses, and comparable diseases prevention reduces the labour for No. one more than 80%.
The 4th, this packing of product is reasonable, and the homogeneous mixture of TCCA (Trichloroisocyanuric acid) and tpn and assistant agent is packed respectively, has guaranteed the stability of medicament, and high chlorine effective ingredient is difficult for descending, and the shelf-life can reach 2 years, and storage and transportation are also safer; In addition, though compound TCCA (Trichloroisocyanuric acid) fumigant is similar to TCCA (Trichloroisocyanuric acid) fumigant cost, as the anti-stiff sterilization of silkworm rearing season, its amount ratio TCCA (Trichloroisocyanuric acid) fumigant reduces half, so its drug cost also reduces nearly about 50%.
In sum, this product have sterilization spectrum wide, efficient, to silkworm safety, can make disinfectant for silkworm room and ware, can make the silkworm body and seat disinfectant again, be specially adapted to the anti-stiff sterilization of silkworm rearing season, easy to use, laborsaving, saving of labor, cost is lower, smog is evenly distributed, the packing of product is reasonable, and characteristics such as long shelf-life have realized goal of the invention.
Embodiment
With the test example the present invention is described in further detail by the following examples.
Embodiment is supplied the explanation of examination material:
TCCA (Trichloroisocyanuric acid) (abbreviation trichlorine) Zhucheng, Shandong Tai Shengang lignify worker Co., Ltd produces, and contains available chlorine 90.37%;
Jiangyin City SuLi Fine Chemical Co., Ltd, tpn Jiangsu produces, and content is 90%;
Wood dust, carbon dust are crossed No. two (24 order) sieve;
Flour is commercially available;
Talcum powder is commercially available, crosses No. two (24 order) sieve;
Zeolite powder Zhejiang refreshing stone ore industry Co., Ltd produces, and crosses No. two (24 order) sieve;
The people live in plenty in white bole Nanjing, and excellent Tu Chang produces;
Embodiment 1:(fumigating disinfectant preparation 1)
(1) tpn, wood dust and talcum powder are crossed No. two (24 order) sieve back, weigh 4.5g, 7.5g and 8g respectively respectively, by formula rate put into mix in the Multidimensionblender after, be packaged into parcel with packing inside bag, weight is the 20g/ bag;
(2) TCCA (Trichloroisocyanuric acid) is crossed the 30g that weighs after No. two (24 order) sieves, be packaged into big bag with packing inside bag, weight is the 30g/ bag;
(3) with the parcel of step (1) and the big bag of step (2), all place in the outer packaging bag, seal, check, put in storage disinfectant products.
When this product used, by the silkworm room disinfection of tools for silkworm rearing, consumption was 5g/M
3, anti-stiff sterilization in silkworm rearing season and a small bundle of straw, etc. for silkworms to spin cocoons on, consumption is 0.5g/M
3The dosage requirement, in airtight silkworm room, two bag medicinal powder are torn along sealing part, parcel medicinal powder is poured in the big bag medicinal powder, clutched sack, shake up, light bag angle or medicinal powder is poured on the paper, light medicinal powder and can produce a large amount of full of smoke whole indoorly, the pathogene of silkworm virus disease, mycosis, bacteriosis, protozoosis etc. is all had killing action, the sootiness ventilation of windowing after 30 minutes.
Embodiment 2:(fumigating disinfectant preparation 2)
The proportioning weight of each component of this fumigating disinfectant is: TCCA (Trichloroisocyanuric acid) 45g, tpn 6.5g, flour 10.5g, potter's clay powder 12g, all the other preparations, using method all are same as embodiment 1.
Embodiment 3:(fumigating disinfectant preparation 3)
The proportioning weight of each component of this fumigating disinfectant is: TCCA (Trichloroisocyanuric acid) 60g, tpn 9g, carbon dust 15g, zeolite powder 16g, all the other preparations, using method all are same as embodiment 1.
Following test example is supplied to try the explanation of material:
(1) for the examination material
Compound TCCA (Trichloroisocyanuric acid) fumigating disinfectant (being called for short the compound fumigant, commodity smoked disappearing only by name): press the listed dosage preparation of form;
The TCCA (Trichloroisocyanuric acid) fumigant is prepared voluntarily by prescription and production technology;
The first silkworm pharmaceutical factory, Deqing of diseases prevention produces;
For trying the silkworm kind:
Be to produce and go up existing recommended variety, provide by the Silk Worm Inst Zhejiang Prov. Agriculture Science Academy; Wherein: " Qiu Feng * white jade " adopted in different proportionings and the test of dosage fumigant, and the former silkworm kind that production of hybrid seeds influence is tested is rich a, white jade of autumn; On-site disinfection test adopts " cyanines pine * bright moon ", " summer
7* the summer
6";
For trying cause of disease:
Preserve for the Silk Worm Inst Zhejiang Prov. Agriculture Science Academy subculture, be made into the bacterium liquid of various variable concentrations during test.Cause of disease comprises:
1) white muscardine fungi: belong to Fungi Imperfecti (Fungi Imperfeeti), Moniliales (Moniliales), Moniliaceae (Moniliaceae) Beauveria [Beauveria bassiana (Balsamo) Vuillemin] abbreviation B.b, be made into 5 * 10 during test
7Individual spore/ml.
2) Aspergillus flavus: belong to Fungi Imperfecti, Moniliales, Moniliaceae, Eurotium (Aspergillusflavus Link) abbreviation A.f, be made into 4 * 10
7Individual spore/ml.
3) nuclear polyhedrosis virus; Be the bombyx mori nuclear polyhydrosis virus (Bombyx mori nuolear polyhedrossis virus is called for short NPV) of Rhabdoviridae (Baculoviridae), for examination, be made into 3 * 10 during test with polygonal figure (NPB)
7Individual/ml polyhedron suspension.
4) cytoplasmic polyhedrosis virus: claim that again (Bombyx mori ctyoplasmicpolyhed rosis virus is called for short silkworm (CPV) to mind-intesting cytoplasmic polyhedrosis virus, belongs to arc reovirus virus section (Reoviridae) and supplies examination with polygonal figure (CPB), is made into 2 * 10 during test
7Individual/ml polyhedron suspension.
5) black chest sepsis bacterium (Bacillus sp) is a kind of escherichia coli of bacillus, is called for short B.SP, is made into 1 * 10 during test
9Individual/ml bacterium liquid.
6) husky thunder iron Salmonella (silkworm already goes up the clever bacterium of common name) is a kind of brevibacterium of husky thunder iron Bordetella (Serratiamaraescens bizio is called for short SM), is made into 1 * 10 during test
9Individual/ml bacterium liquid.
7) particulate spore: belong to protozoa, the monofilament Sporozoa, Wei Bao Chong Gang, the microsporidian order, monofilament sporozoit suborder, microsporidian belongs to.Formal name used at school Nosema bombycis Naegeli is called for short N.b;
8) Bt: Bacillaceae (Bacillaceue), bacillus, Su Yun gold bar mutation finally (being called for short mutation of soldier), formal name used at school is Bacillus thuringiensis Var sotto.
Test different proportionings of routine 1:(and dosage fumigant to the test of fungi killing effect)
1) microbiological test: with B.b, A.f cause of disease liquid 100 μ l drip (plate medium method for making: the medium heating and melting that will specialize in fungi or bacterial growth on plate fungi and bacteria culture media respectively, get 15ml and pour diameter 9cm plate culture dish cooled and solidified into), push away flat with the sterilization glass bar, put on the silkworm room, in, inferior different parts, respectively with airtight smoked the disappearing 2 hours of the fumigant of different proportionings and various dose, other establishes not sterile zones (poison to district) and do not inoculate check plot (blank district) of equal conditions cause of disease, finish to cover in 28 ℃ of incubators of ware immigration and cultivated investigation colony growth situation 3-7 days.
2) the silkworm body surface carries the bacterium test: with 5 * 10
7Individual/ml and 4 * 10
7The B.b of individual/ml and A.f spore suspension sparge the newly-hatched silkworm body surface equably, upper, middle and lower, dried slightly rearmounted silkworm room, ground different parts, with airtight smoked the disappearing 30 minutes of the fumigant of different proportionings and dosage, preserve moisture and raise conventional raising the after a day, investigate incidence every day, play silkworm investigation to three ages and end.
Result of the test:
1. to the killing effect of silkworm epiphyte cause of disease:
Different proportionings of table 1 and dosage fumigant are to the fungi killing effect
Annotate: 1. "-" expression asepsis growth " ± " expression has indivedual bacterium colonies "+" expression to have a small amount of bacteria growing " ++ " expression bacteria growing good
Test period: 1996.4.1-1996.4.8 disappears the medium that scribbles B.b and A.f 2 hours through airtight the smoking of the fumigant of different proportionings and dosage, and constant temperature culture 7 days the results are shown in Table 1.
2. silkworm rearing season is prevented stiff effect
Test period: 1996.9.1-1996.9.7, behind white deadlock of newly-hatched silkworm body spray and aspergillus spore, with after airtight smoked the disappearing 30 minutes of the fumigant of different proportionings and dosage, ventilation is preserved moisture and is raised after 1 day, normally raises two length of times, the results are shown in Table 2 respectively.
The fumigant silkworm rearing season of different proportionings of table 2 and dosage is prevented stiff effect (strong silkworm rate %)
Annotate: achievement is that the newly-hatched silkworm that carries disease germs is put the mean value of upper, middle and lower, silkworm room, ground diverse location, 50 silkworms in every sub-district, 3 repetitions in the table.
Table 1, table 2 result show, add tpn in the TCCA (Trichloroisocyanuric acid) fumigant, can strengthen the control efficiency to fungal pathogen, and its drug effect strengthens with tpn content.
Test the reasonability test of routine 2:(medicament proportioning)
By behind the abundant mixing of proportioning, point is lighted cigarette with fumicants effective agent and assistant agent, and the content of measuring available chlorine in the back residue of being fuming is lower than 1.0%, shows that the back active ingredient of being fuming is released reasonable mixture ratio substantially.
Test the toxicity test of routine 3:(compound TCCA (Trichloroisocyanuric acid) fumigant) to silkworm
This test comprises following three aspect contents:
1. high dose is smoked disappears to the toxic effect of silkworm:
The newly-hatched silkworm that has just hatched is put 3.74m
35g/M is used in little silkworm room respectively
3, 3g/M
3, 2g/M
3Behind the fumigant of dosage smoked disappear 5h and the 30min, ventilation, the check plot newly-hatched silkworm is put another silkworm room, treat smoked disappear that the district newly-hatched silkworm is smoked and disappear after respectively routine raise to putting on an arrow, investigation silkworm development situation, amount of cocoon crop and cocoon quality, each handles three repetitions.About 200 silkworms in every sub-district.
2. play silkworm in newly-hatched silkworm and each age, the matured silkworm low dosage is smoked disappears to the silkworm physiological effect:
Each handles 3 repetitions, and about 200 silkworms in every sub-district are used this product 0.5g/M respectively when newly-hatched silkworm and each age playing silkworm, matured silkworm
3, 1g/M
3, 1.5g/M
3Smoked the disappearing 30 minutes of dosage after give mulberry, other does not establish smoked the check plot that disappears, and investigates growth, cocoon quality and the silk quality of silkworm.
3. matured silkworm and pupa time, smoked disappearing influenced the production of hybrid seeds:
, white jade rich with the autumn is material to matured silkworm and the pupa of handing over kind, uses 0.5-1.5g/M respectively
3Smoked the disappearing 30 minutes of dosage, after the ventilation, conventional temp. and humidity protection, the strong pupa rate of investigation, moth appearance rate, total ovum number of mate and oviposit " Invest, Then Investigate " and common incubation rate.
Two, result of the test
1. high dose is smoked disappears to the toxicity test of silkworm
Table 3 newly-hatched silkworm 2-5g/M
3Dosage is smoked to disappear to the development impact of silkworm
Table 4 newly-hatched silkworm 2-5g/M
3Smoked disappearing influences cocoon quality
| Smoked dosage (the g/M that disappears 3) | Smoked disappear the time (h) | The rate of cocooing (%) | Ten thousand amount of cocoon crop (kg) | Cocoon weight (g/) | Cocoon shell weight (g/) | Cocoon layer rate (%) |
| 5 | 5.0 | 96 | 14.83 c | 1.76 c | 0.34 c | 19.32 d |
| 5 | 0.5 | 97 | 14.96 c | 1.82 b | 0.37 b | 20.33 c |
| 3 | 5.0 | 97 | 14.33 b | 1.86 b | 0.39 a | 20.97 b |
| 2 | 5.0 | 98 | 16.61 a | 1.88 a | 0.39 a | 20.74 b |
| Contrast | 0 | 96 | 16.86 a | 1.91 a | 0.41 a | 21.45 a |
Newly-hatched silkworm is used 5g/M
3Dosage smoked disappear 5h and 30min dull, stupor all occurs, but promptly revive after the ventilation; Use 5g/M
3The smoked 30min that disappears of dosage, the length of time, hibernating worm body weight, cocoon weight, cocoon shell weight, cocoon weight, cocoon shell weight were lower than the check plot through prolonging 2h, the smoked 5h that disappears, the length of time, hibernating worm body weight, cocoon weight, cocoon shell weight all significantly were lower than the check plot through prolonging 2 days; Use 3g/M
3The smoked 5h that disappears of dosage, silkworm grows normal, and the length of time, hibernating worm body weight, ten thousand amount of cocoon crop, cocoon weight, cocoon shell weight, cocoon layer rates were lower than the check plot through prolonging 2h; Use 2g/M
3The smoked 5h that disappears of dosage, silkworm age be through, hibernating worm body weight and check plot indifference, and cocoon quality is lower than check plot (see Table 3, table 4).
2, newly-hatched silkworm and play that silkworm, matured silkworm low dosage are smoked to disappear to the physiological effect of silkworm each age:
Table 5, table 6 result show that newly-hatched silkworm, each age are played silkworm, matured silkworm is used 0.5g/M
3And 1g/M
3Dosage sootiness 30min has no adverse effects 1.5g/M to silkworm development, amount of cocoon crop and cocoon quality, silk quality
3The smoked district that disappears of dosage, to silkworm development, the rate of cocooing with contrast indifference, but ten thousand amount of cocoon crop, cocoon weight, the lower slightly check plot of cocoon shell weight do not have obvious influence to silk quality.
Table 5 low dosage is smoked to disappear to silkworm development and cocoon quality influence
| The smoked dosage g/M that disappears 3 | Full age is through (d:h) | The rate of cocooing (%) | Ten thousand amount of cocoon crop (kg) | Cocoon weight (g/) | Cocoon shell weight (g/) | Cocoon layer rate (%) |
| 0.5 | 22:05 | 93.50 a | 14.86 a | 1.76 a | 0.3476 a | 19.75 a |
| 1.0 | 22:05 | 94.00 a | 14.70 ab | 1.74 a | 0.3456 b | 19.86 a |
| 1.5 | 22:05 | 93.00 a | 14.65 b | 1.70 b | 0.3327 b | 19.51 b |
| Contrast | 22:05 | 89.00 b | 14.84 a | 1.75 a | 0.3486 a | 19.82 a |
Smoked the disappearing of table 6 low dosage influences silk quality
| The smoked dosage g/M that disappears 3 | Silk long (m) | Reelability percentage (%) | Length of non broken filament (m) | The size of cocoon filament (dcn) |
| 0.5 | 1029 | 66.10 | 742 | 2.18 |
| 1.0 | 1018 | 65.48 | 738 | 2.29 |
| 1.5 | 1008 | 65.63 | 739 | 2.20 |
| Contrast | 1019 | 67.39 | 758 | 2.21 |
3. former silkworm matured silkworm and pupa time, smoked disappearing tested vitality and germplasm influence
Table 7 matured silkworm and smoked the disappearing of low concentration in pupa time influence vitality
Smoke in former silkworm matured silkworm of table 8 and pupa time and disappear the influence of first generation cross-fertilize seed ovum amount
Table 7 is the result show, uses 0.5g-1.5g/M in former silkworm matured silkworm and pupa time
3The smoked 30min that disappears, the rate of cocooing is higher than the check plot, and moth appearance rate, pupa time illustrate that through identical with the check plot former silkworm matured silkworm and pupa at the smoked 30min that disappears of above-mentioned dosage range, have no adverse effects to its vitality.The every data survey of egg laying amount shows (table 8), and each smoked total ovum number of Qu that disappears, good ovum number average are lower than the check plot, do not have significant difference through biostatistics, and each district of good ovum rate is all more than 98%, and the incubation rate quadrature is more than 98%, and reciprocal cross is more than 97%, no significant difference.
Above-mentioned test shows:
1. newly-hatched silkworm is used 5g/M
3Dosage smoked disappear 5h and 30min do not have lethal effect to silkworm, can normally eat mulberry, cocoon, but process prolonged the length of time, and hibernating worm body weight, cocoon weight, cocoon shell weight, cocoon layer rate are lower than and significantly are lower than the check plot; 3g/M
3The smoked 5h that disappears of dosage, silkworm grows normal, and the length of time, hibernating worm body weight, ten thousand amount of cocoon crop, cocoon weight, cocoon shell weight, cocoon layer rates were lower than the check plot through prolonging 2h; 2g/M
3The smoked 5h that disappears of dosage, silkworm age process, hibernating worm body weight and check plot indifference, cocoon quality is lower than the check plot.
2, common species newly-hatched silkworm, each age are played silkworm, matured silkworm is used 0.5g/M
3And 1g/M
3The smoked 30h that disappears of dosage has no adverse effects 1.5g/M to silkworm development, cocoon quality, silk quality
3The smoked 30h that disappears of dosage has no adverse effects to silkworm development, the rate of cocooing, but ten thousand amount of cocoon crop, cocoon shell weight, cocoon layer rates are lower than contrast, and there were significant differences, and silk quality is not had obvious influence.
3. former silkworm matured silkworm and pupa are used 0.5g/M respectively
3, 1g/M
3, 1.5g/M
3The smoked 30h that disappears of dosage does not all make significant difference to vitality and production of hybrid seeds achievement.
Test routine 4:(fumigant microorganism drug effect and on-the-spot disinfection effect test)
This test is made into 2 * 10 with each cause of disease
7Individual/the ml spore suspension, the fumigation environment: volume is 3.78m
3Little silkworm room.
This test comprises following content:
1. microbiological test method: test period 1996.4.14～1996.4.21; Cause of disease liquid 100 μ l such as Bb, AF, B.SP, Bt and SM are dripped respectively on plate fungi and bacteria culture media, push away flat with the sterilization glass bar, put diverse locations such as upper, middle and lower, silkworm room, left, center, right, respectively with the airtight smoked 2h that disappears of the compound fumigant of various dose; TCCA (Trichloroisocyanuric acid) fumigant with various dose compares; Other establishes not sterile zones (poison to district) and do not inoculate check plot (blank district) of equal conditions cause of disease, finishes cover in the incubator that ware moves into 28 ℃ cultivation 3-7 days, investigates the colony growth situation.
2. surface of glass slide is carried bacterium test method(s): test period 1996.5.1-1996.6.1; Get the suspension 100 μ l of NPB, CPB, N.b, make 3 * 4cm cause of disease smear respectively, rearmounted 3.78m dries in the shade
3Upper, middle and lower, young silkworm chamber, left, center, right, ground diverse location with airtight smoked the disappearing 5 hours of various dose compound fumigant, take out and scrape cause of disease with aqua sterilisa, are applied on the mulberry leaf blade back, freshen food to newly-hatched silkworm.NPB, CPB test region routine are raised 2-3 the length of time, and raise to putting on an arrow the N.b test region, and the investigation incidence compares medicament with the TCCA (Trichloroisocyanuric acid) fumigant, and other establishes poison to distinguishing and the blank district, and each handles three repetitions, 50 silkworms in every sub-district.
3, the silkworm body surface carries bacterium test method(s): test period 1996.9.1-1996.9.7; With 5 * 10
7Individual/ml and 4 * 10
7The B.b of individual/ml and A.f conidial suspension sparge the newly-hatched silkworm body surface uniformly, upper, middle and lower, dried slightly rearmounted silkworm room, ground (each minute left, center, right) diverse location, with the airtight smoked 30h that disappears of various dose compound fumigant, preserve moisture and raise conventional raising the after a day, investigate incidence every day, investigation plays silkworm to three ages.Compare medicament No. one with trichlorine isocyanide fumigant and diseases prevention, other establishes poison to district and blank district.
Result of the test is:
1. the compound fumigant is to the killing effect of silkworm epiphyte and bacteria pathogeny
Through the smoked 2h that disappears of the fumigant of various dose, constant temperature culture 7 days the results are shown in Table 9, table 10 to the compound fumigant to the medium that scribbles silkworm epiphyte cause of disease and bacteria pathogeny.
Table 9 compound fumigant is to the killing effect of silkworm epiphyte cause of disease
Annotate: "-" expression bacterium does not grow, and a small amount of bacterium colony of " ± " expression generates, and "+" expression has bacterium colony to generate, and " ++ " expression bacteria growing is good; The compound fumigant is embodiment 2 products.
The killing effect that table 10 compound fumigant is former to bacterial disease of domestic silkworm
Annotate: "-" expression bacterium does not grow, and a small amount of bacterium colony of " ± " expression generates, and "+" expression has bacterium colony to generate, and " ++ " expression bacteria growing is good
Table 9, table 10 result show 0.25g/M
3The airtight smoked 2h that disappears of dosage compound fumigant can kill white deadlock of silkworm and aspergillus spore, and the TCCA (Trichloroisocyanuric acid) fumigant need be used 1g/M
3Dosage just can kill white deadlock and aspergillus spore, illustrate in the fumigant add tpn after, strengthened killing effect to fungal pathogen, the compound fumigant is identical with the TCCA (Trichloroisocyanuric acid) fumigant to the former Disinfection Effect of bacterial disease of domestic silkworm, 1g/M
3Dosage dosage is smoked can the kill bacteria cause of disease when disappearing 2h.
2. the compound fumigant is to the Disinfection Effect of silkworm virus polyhedron and particulate spore
The slide that scribbles various cause of diseases puts the silkworm room diverse location, with behind the airtight smoked 5h that disappears of various dose fumigant, wash down cause of disease with water and freshen food to silkworm, the results are shown in Table 11.
The Disinfection Effect (incidence of disease %) of table 11 pair silkworm virus polyhedron and particulate spore
Annotate: achievement is put the mean value of upper, middle and lower, silkworm room, left, center, right different parts, 50 silkworms in every district, three repetitions for the cause of disease slide in the table
Table 11 is the result show, the compound fumigant is consistent with particulate spore Disinfection Effect to silkworm mind-intesting cytoplasmic polyhedrosis polyhedron with the TCCA (Trichloroisocyanuric acid) fumigant, uses 1g/M
3The smoked 5h that disappears of dosage fumigant has remarkable Disinfection Effect, 2g/M to silkworm blood type pus illness polyhedron and particulate spore
3Above dosage can thoroughly be killed, and the mind-intesting cytoplasmic polyhedrosis polyhedron is through 4g/M
3The smoked 5h that disappears of dosage has good Disinfection Effect, 5g/M
3The smoked 5h that disappears of dosage can kill fully.
3. stiff Disinfection Effect is prevented at compound fumigant silkworm rearing season scene
Behind white deadlock of newly-hatched silkworm body spray and the aspergillus spore, with the smoked 30min that disappears of various dose fumigant, ventilation is preserved moisture to raise after one day and is normally raised two length of times, the results are shown in Table 12 respectively.Show 0.5g/M
3Dosage compound fumigant is to the smoked 30min that disappears of the newly-hatched silkworm that carries disease germs, and dialogue control efficiency stiff and aspergillus is respectively 100% and 96%, drug effect and 1g/M
3The TCCA (Trichloroisocyanuric acid) fumigant of dosage is (dosage reduces half) quite, and the control efficiency of aspergillus is better than diseases prevention No. one.
Table 12 compound fumigant silkworm rearing season is prevented stiff effect (incidence of disease %)
Annotate: 1. achievement is that the newly-hatched silkworm that carries disease germs is put the mean value of upper, middle and lower, silkworm room, ground diverse location, 50 silkworms in every sub-district, three repetitions in the table
Test the relation test of routine 5:(compound fumigant Disinfection Effect and cause of disease position)
Test period: 1996.9.1-1996.9.7;
(1) is coated with bacterium culture medium and puts different parts through 0.25g/M
3The smoked 2h that disappears of dosage compound fumigant, 28 ℃ of constant temperature culture 7 days the results are shown in Table 13.(2) behind white deadlock of newly-hatched silkworm body spray and the aspergillus spore, put different parts through 0.25g/M
3The smoked 30min that disappears of dosage compound fumigant, ventilation is preserved moisture to raise after one day and is normally raised two length of times, the results are shown in Table 14.Table 13, table 14 show, the compound fumigant is smoked when disappearing, and smog is evenly distributed upper, middle and lower diverse location drug effect unanimity.
The smoked result that disappears of the fungal spore of table 13 different parts
Annotate: "-" expression bacterium does not grow, and a small amount of bacterium colony of " ± " expression generates, and "+" expression has bacterium colony to generate, and " ++ " expression bacteria growing is good
The table 14 smoked result (incidence of disease %) that disappears of newly-hatched silkworm diverse location that carries disease germs
Annotate: achievement is handled the mean value of three repetitions for each in the table
Test the test of routine 6:(compound fumigant Disinfection Effect and temp. and humidity relation)
Test period 1996.11.20-1996.11.27; Under 25 ℃ of temperature, 75% humidity and 15 ℃ of temperature, 65% damp condition, use 0.25g/M
3Dosage compound fumigant to the airtight smoked 2h that disappears of the plate medium that scribbles fungal spore after, put 28 ℃ of constant incubators and cultivated 7 days, the results are shown in Table 15, show that the medium that scribbles fungi is used 0.25g/M under different temperature and humidity conditions
3The smoked 2h that disappears of dosage has killing action to B.b and A.f, illustrates that the sterilization effect of this fumigant and temp. and humidity relation are little.
Table 15 compound fumigant Disinfection Effect and temp. and humidity relation
Claims (4)
1, a kind ofly be used for the fumigating disinfectant that silkworm already produces, it is characterized in that the component of this fumigating disinfectant and the weight portion of each component are:
25～60 parts of TCCA (Trichloroisocyanuric acid)
3～10 parts of tpns
6～18 parts of combustion adjuvants
7～20 parts of fire retardant chemicals.
2, fumigating disinfectant according to claim 1 is characterized in that the component of described fumigating disinfectant and the weight portion of each component are: 45 parts of TCCA (Trichloroisocyanuric acid), 6.5 parts of 90% tpns, 10.5 parts in combustion adjuvant flour, 12 parts in fire retardant chemical potter's clay powder.
3, fumigating disinfectant according to claim 1 is characterized in that described combustion adjuvant is wood dust, flour or carbon dust; Described fire retardant chemical is talcum powder, potter's clay powder or zeolite powder.
4, a kind of method for preparing the described fumigating disinfectant of claim 1 is characterized in that carrying out according to the following steps:
(1) after tpn, combustion adjuvant and fire retardant chemical being crossed 24 mesh sieves respectively, weighing, by formula rate drop into mix in the Multidimensionblender after, weigh, be packaged into parcel with packing inside bag;
(2) TCCA (Trichloroisocyanuric acid) is crossed 24 mesh sieves, weighed, be packaged into big bag with packing inside bag;
(3) with the parcel of step (1) and the big bag of step (2), place in the outer packaging bag, seal, check, put in storage disinfectant products.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101336630B (en) * | 2008-08-12 | 2010-12-15 | 闫现广 | Fumigant for silkworm |
| CN102369960B (en) * | 2010-08-20 | 2013-10-02 | 河南亚卫动物药业有限公司 | Solid fumigation disinfectant and preparation method thereof |
| CN102835425A (en) * | 2011-06-21 | 2012-12-26 | 唐山宏基伟业生物工程有限公司 | Preparation method of fumigating agent |
| CN109452270A (en) * | 2018-11-14 | 2019-03-12 | 天津市诺维动物药业有限公司 | Sodium dichloroisocyanurate fumigant |
| CN113796387B (en) * | 2021-09-27 | 2022-09-20 | 陆琳 | Method for preventing and treating myiasis of silkworms |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1016264B (en) * | 1989-04-17 | 1992-04-15 | 卡托维兹南方电力工业区上瓦济斯卡电厂 | Method and automatic optimization system of combustion processes in thermal objects |
| CN1454476A (en) * | 2002-12-13 | 2003-11-12 | 国家农产品保鲜工程技术研究中心（天津） | Method of inhibiting rottenness during storage and production process of agricultural products |
| CN1489905A (en) * | 2002-10-15 | 2004-04-21 | 烟台神龙蚕用药业科技开发有限公司 | Powdery disinfectant for sterilizing silk-worm body |
| CN1843158A (en) * | 2006-04-28 | 2006-10-11 | 莫如章 | Composite silkworm feed stuff and its producing method |
| CN101011067A (en) * | 2007-01-30 | 2007-08-08 | 中国农业科学院蚕业研究所 | Composite disinfectant for silkworm |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1016264B (en) * | 1989-04-17 | 1992-04-15 | 卡托维兹南方电力工业区上瓦济斯卡电厂 | Method and automatic optimization system of combustion processes in thermal objects |
| CN1489905A (en) * | 2002-10-15 | 2004-04-21 | 烟台神龙蚕用药业科技开发有限公司 | Powdery disinfectant for sterilizing silk-worm body |
| CN1454476A (en) * | 2002-12-13 | 2003-11-12 | 国家农产品保鲜工程技术研究中心（天津） | Method of inhibiting rottenness during storage and production process of agricultural products |
| CN1843158A (en) * | 2006-04-28 | 2006-10-11 | 莫如章 | Composite silkworm feed stuff and its producing method |
| CN101011067A (en) * | 2007-01-30 | 2007-08-08 | 中国农业科学院蚕业研究所 | Composite disinfectant for silkworm |
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