CN1387764A

CN1387764A - Insecticide suspension of beet noctuid polyhedron virus

Info

Publication number: CN1387764A
Application number: CN 02115908
Authority: CN
Inventors: 张忠信; 张小霞; 牛国栋; 张友清
Original assignee: Wuhan Institute of Virology of CAS
Current assignee: Wuhan Institute of Virology of CAS
Priority date: 2002-05-31
Filing date: 2002-05-31
Publication date: 2003-01-01
Anticipated expiration: 2022-05-31
Also published as: CN1176213C

Abstract

The present invention discloses beet noctuid nuclear polyhedrosis virus insect killing suspending agent, in which the virus bearer content is 5-20 hundred million PIB/ml., itis composed of chlorbenzuron analogue chlorfluazuron, aromatic amino acid tyrosine, fluorescent whitener, dispersant, glycerin, sterilized water. The present invention is harmless to man and livestock and other higher animals, has no pollution to environment and has no pesticide residue.

Description

Insecticide suspension of beet noctuid polyhedron virus

Technical field

The present invention relates to biological insecticides, more specifically relate to a kind of insecticide suspension of beet noctuid polyhedron virus, be applicable to biological control.

Background technology

Beet armyworm is nearly hundred kinds of crops such as the important pests on China's agricultural, main harm vegetables, soybean, causes heavy economic losses for vegetable grower and peasant.In the past, beet armyworm is only taken place in China south, and harm is confined to the vegetables field, and is intermittent the generation.In recent years, along with the influence of global warming and other factors, generation of beet armyworm and harm are the trend that increases the weight of year by year.At present, beet armyworm not only becomes the primary pest on China vegetables, and seriously outburst on crops such as soybean, cotton.The control of beet armyworm mainly relies on chemical pesticide, but the beet armyworm reproduction speed is fast, the annual generation many generations, and chemical pesticide also needs frequent the use.Because a large amount of uses of chemical pesticide, beet armyworm has produced serious resistance to the number of chemical agricultural chemicals.In addition, use the chemical pesticide serious environment pollution in a large number vegetables Tanaka, residue of pesticide constitute directly threat to human beings'health.

Baculovirus insecticides, harmless, free from environmental pollution because it is to host insect good disinsection effect to people and animals and other higher mammal, do not injure natural enemy, be one of main biologic product of prevention target insect.But compare with chemical pesticide, baculovirus insecticides desinsection speed is slow, and the field is used and just can be seen positive effect in 4-7 days.This defective of baculovirus insecticides is the principal element of restriction insect virus pesticide development.In order to improve the desinsection speed of baculoviral, existing method mainly contains two kinds, the one, make up recombinant baculovirus, in the baculoviral genome, insert genes such as scorpion neurotoxin gene, mite neurotoxin gene with gene engineering method, thereby improve baculoviral insecticidal speed, but this method cost cost height, unstable result, simultaneously because the production of baculoviral can only be leaned on host insect live body propagation at present, recombinant virus desinsection speed mainly is the effect that toxin gene is expressed soon, rather than the effect of viral fast breeding.In virus production, recombinant virus is difficult to obtain a large amount of progeny virus, and under present condition, recombinant virus is difficult to carry out batch production production.The another kind of method that improves baculoviral insecticidal speed is to add synergist in viral insecticide.Mostly with the synergist of chemical pesticide as it, this has just lost the characteristic of biological insecticides to existing viral insecticide, also is not suitable for using in a large number on vegetables.Fluorescent whitening agent also has certain synergistic effect to baculoviral, but need concentration that obvious synergistic effect is just arranged above 1%, because viral insecticide dilutes 500-800 doubly when reality is sprayed, make the fluorescent brightening agent concentration reach 1% can't prepare in formulation.In recent years, the synergist of some biologically actives uses in baculovirus insecticides, the cotton bollworm nucleopolyhedrosis virus adds benzoylphenyl urea can make the half lethal time LT50 of virus insect disinfestation shorten to 2.66 days like thing, though but benzoylphenyl urea can improve the half lethal time of cotton bollworm nucleopolyhedrosis virus desinsection like thing, host insect virus disease lethality rate reaches 90% still needs 5-6 days.Baculoviral is that virus has certain susceptibility to ultraviolet light, the easy loss of activity of being exposed to the sun in the sun for a long time as another defective of insecticide.In order to remedy this defective, need in viral insecticide, add bright protective agent.The bright protective agent that adds in virus insect disinfestation wetting powder and other formulation mainly is active carbon and the Congo red dyestuff that waits, and these bright protective agents make the appearance luster of formulation poor, and light protects effect also undesirable.

At present, the dosage form research of baculovirus insecticides and application lag significantly behind the formulation of other agricultural chemicals, and viral insecticide is now still based on wetting powder, and other has a spot of emulsion.These formulations are used inconvenient, and the auxiliary agent maldistribution is easy to generate residual and poisoning.

Summary of the invention

The object of the present invention is to provide a kind of insecticide suspension of beet noctuid polyhedron virus, compatibility is reasonable, and is easy to operate, and good disinsection effect can effectively have been controlled generation and the harm of beet armyworm.

In order to achieve the above object, the present invention adopts following technical measures: Main Ingredients and Appearance is a Spodoptera exigua nucleopolyhedrovirusand, Spodoptera exigua nucleopolyhedrovirusand belongs to the baculoviral coe virus, be the World Food Programme recommend can be used for one of safe biologic of biological control.Under Electronic Speculum, observe, virus polyhedron form is variforms such as circle, quadrangle, pentagon, its mean size is 1.45um, it is shaft-like that virion is, the blunt circle in two ends, size is 347nm * 117.5nm, and virion is a multinuclear capsid embedding type, contains 1-4 nucleocapsid in each virion.Spodoptera exigua nucleopolyhedrovirusand obviously is better than other baculoviral to the desinsection speed of targeted insect.Spodoptera exigua nucleopolyhedrovirusand to 1,2,3 age beet exigua larvae half lethal time (LT50) all less than 4 days, have good using value.Spodoptera exigua nucleopolyhedrovirusand is forgiven content 5-20 hundred million PIB/ml (protein inclusion body or viral inclusion body) in the formulation; the benzoylphenyl urea of tool insect specific biological activity is like thing fluorine pyridine urea 0.1-0.2%; virus bright protective agent aromatic amino acid tryptophan 0.01-0.1%; refining fluorescent whitening agent 0.2-0.5%; dispersant 1-2%; glycerine 2-5%, sterile water 91.2-96.7%.(dispersant is L602 or LH656 or L656 or is the agricultural chemicals assistant agent like thing fluorine pyridine urea, refining fluorescent whitening agent, dispersant to add glycerine, aromatic amino hydroxyphenylaminopropionic acid, benzoylphenyl urea during preparation successively in Spodoptera exigua nucleopolyhedrovirusand, compound is multiple compound), aqua sterilisa, packing after stirring can obtain suspending agent.

The present invention compared with prior art, have the following advantages and effect: this formulation has promptly kept the biological insecticides characteristic of viral insecticide, harmless to people and animals and higher mammal, be suitable on vegetables, using in a large number, accelerate virus insect disinfestation speed again greatly, make the desinsection time of viral insecticide shorten to 3-4 days, insecticidal effect 85-90% is used in the field.Virus insect disinfestation suspension agent has overcome the defective of wetting powder and emulsifiable concentrate, and auxiliary agent is easy to disperse, and the field is used more convenient, does not have residual and environmental pollution.Benzoylphenyl urea not only can make the LT50 of virus insect disinfestation shorten to about 2 days like thing as the synergist of Spodoptera exigua nucleopolyhedrovirusand, and beet exigua larvae virus disease lethality rate reaches time of 90% and shortens to 4.2 days.This has remarkable advantages in actual applications.

Specific embodiment example 1: Spodoptera exigua nucleopolyhedrovirusand 1,000,000,000 PIB/ml glycerine 3% aromatic amino hydroxyphenylaminopropionic acid 0.1% benzoylphenyl urea is like thing fluorine pyridine urea 0.025% refining fluorescent whitening agent 0.2% dispersant 2% sterilized water 94.675%

Add above each composition during preparation successively; The back packing that stirs, product is a kind of milky suspending agent. example 2: Spodoptera exigua nucleopolyhedrovirusand 600,000,000 PIB/ml glycerine 5% aromatic amino acid tryptophan 0.1% benzoylphenyl urea is like thing fluorine pyridine urea 0.045% refining fluorescent whitening agent 0.5% dispersant 2% sterilized water 92.355%

Compound method is identical with example 1. examples 3: Spodoptera exigua nucleopolyhedrovirusand 800,000,000 PIB/ml glycerine 3% aromatic amino hydroxyphenylaminopropionic acid 0.1% benzoylphenyl urea is like thing fluorine pyridine urea 0.01% refining fluorescent whitening agent 0.3% dispersant 2% sterilized water 94.59%

Compound method is identical with example 1.

Claims

1, a kind of insecticide suspension of beet noctuid polyhedron virus, it is made by following materials of weight proportions:

Spodoptera exigua nucleopolyhedrovirusand 5-20 hundred million PIB/ml

Glycerine 2-5%

Aromatic amino acid tryptophan 0.01-0.1%

Benzoylphenyl urea is like thing fluorine pyridine urea 0.1-0.2%

Refining fluorescent whitening agent 0.2-0.5%

Dispersant 1-2%

Sterile water 92.2-96.69%.

2, a kind of insecticide suspension of beet noctuid polyhedron virus according to claim 1 is characterized in that the weight proportion of raw material is:

Spodoptera exigua nucleopolyhedrovirusand 1,000,000,000 PIB/ml

Glycerine 3%

Aromatic amino hydroxyphenylaminopropionic acid 0.1%

Benzoylphenyl urea is like thing fluorine pyridine urea 0.025%

Refining fluorescent whitening agent 0.2%

Dispersant 2%

Sterile water 94.675%.

3, a kind of insecticide suspension of beet noctuid polyhedron virus according to claim 1 is characterized in that the weight proportion of raw material is:

Spodoptera exigua nucleopolyhedrovirusand 600,000,000 PIB/ml

Glycerine 5%

Aromatic amino acid tryptophan 0.1%

Benzoylphenyl urea is like thing fluorine pyridine urea 0.045%

Refining fluorescent whitening agent 0.5%

Dispersant 2%

Sterile water 92.355%

4, a kind of insecticide suspension of beet noctuid polyhedron virus according to claim 1 is characterized in that the weight proportion of raw material is:

Spodoptera exigua nucleopolyhedrovirusand 800,000,000 PIB/ml

Glycerine 3%

Aromatic amino hydroxyphenylaminopropionic acid 0.1%

Benzoylphenyl urea is like thing fluorine pyridine urea 0.01%

Refining fluorescent whitening agent 0.3%

Dispersant 2%

Sterile water 94.59%