CN100528895C - 精制浓缩酶溶液的方法 - Google Patents
精制浓缩酶溶液的方法 Download PDFInfo
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- CN100528895C CN100528895C CNB2004800031362A CN200480003136A CN100528895C CN 100528895 C CN100528895 C CN 100528895C CN B2004800031362 A CNB2004800031362 A CN B2004800031362A CN 200480003136 A CN200480003136 A CN 200480003136A CN 100528895 C CN100528895 C CN 100528895C
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- enzyme
- solution
- concentrated
- activity
- proteinase
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Abstract
Description
活性[1000HPU/g] | 溶液的固体含量[vol%] |
0 | 0.2 |
200 | 0.5 |
400 | 1.2 |
600 | 1.5 |
800 | 3.0 |
1000 | 7.5 |
活性[100TAU/g] | 溶液的固体含量[vol%] |
0 | 0.5 |
100 | 1.0 |
200 | 1.5 |
300 | 2.5 |
400 | 3.2 |
500 | 5.0 |
Claims (40)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10304066A DE10304066B4 (de) | 2003-01-31 | 2003-01-31 | Verfahren zur Veredelung konzentrierter Enzymlösungen |
DE10304066.8 | 2003-01-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1745093A CN1745093A (zh) | 2006-03-08 |
CN100528895C true CN100528895C (zh) | 2009-08-19 |
Family
ID=32747525
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004800031362A Expired - Fee Related CN100528895C (zh) | 2003-01-31 | 2004-01-23 | 精制浓缩酶溶液的方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US20050266542A1 (zh) |
EP (1) | EP1587824B1 (zh) |
CN (1) | CN100528895C (zh) |
DE (1) | DE10304066B4 (zh) |
DK (1) | DK1587824T3 (zh) |
ES (1) | ES2533001T3 (zh) |
HK (1) | HK1085747A1 (zh) |
WO (1) | WO2004067557A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10360841A1 (de) * | 2003-12-20 | 2005-07-14 | Henkel Kgaa | Helle, stabile, staub- und geruchsarme Enzymgranulate |
JP5051680B2 (ja) * | 2004-08-09 | 2012-10-17 | ナノミストテクノロジーズ株式会社 | 石油の分離方法と分離装置 |
DE102004047776B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Gegen Di- und/oder Multimerisierung stabilisierte Alpha-Amylase-Varianten, Verfahren zu deren Herstellung sowie deren Verwendung |
DE102004047777B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung |
US9133424B2 (en) | 2011-12-16 | 2015-09-15 | Ecolab Usa Inc. | Stabilization and activation of protease for use at high temperature |
CN104204183A (zh) | 2012-03-29 | 2014-12-10 | 诺维信公司 | 酶用于制备水溶性膜的用途 |
CA3087284C (en) * | 2018-01-26 | 2023-05-02 | The Procter & Gamble Company | Water-soluble unit dose articles comprising enzyme |
KR20200085891A (ko) | 2018-01-26 | 2020-07-15 | 더 프록터 앤드 갬블 캄파니 | 향료를 포함하는 수용성 단위 용량 물품 |
CN111321126A (zh) * | 2020-02-26 | 2020-06-23 | 湖南苏仙瑶岭生态休闲农业开发有限公司 | 一种生物酶制作方法 |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9019919D0 (en) * | 1990-09-12 | 1990-10-24 | Delta Biotechnology Ltd | Purification of proteins |
US5625041A (en) * | 1990-09-12 | 1997-04-29 | Delta Biotechnology Limited | Purification of proteins |
US4806346A (en) * | 1986-12-16 | 1989-02-21 | American Home Products Corporation | Method for isolation of antigen specific immunoglobulin |
JPH02502786A (ja) * | 1987-12-23 | 1990-09-06 | ギスト ブロカデス ナームローゼ フェンノートチャップ | 精製された工業酵素及びその調製方法 |
EP0365858B1 (en) * | 1988-10-26 | 1994-11-30 | American Cyanamid Company | Process for reducing the aggregate content of growth hormone-like materials |
US5688290A (en) * | 1989-10-19 | 1997-11-18 | Genencor International, Inc. | Degradation resistant detergent compositions based on cellulase enzymes |
US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
CA2032255A1 (en) * | 1990-12-14 | 1992-06-15 | Otto Sova | Method and apparatus for separating biological substances and organic compounds in solution |
US5405767A (en) * | 1992-04-08 | 1995-04-11 | Solvay Enzymes, Inc. | Purified enzyme concentrate and method of preparation |
DE69333463D1 (de) * | 1992-05-18 | 2004-05-06 | Genencor Int | Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen |
DE4322229A1 (de) * | 1993-07-05 | 1995-01-12 | Cognis Bio Umwelt | Umhüllte Enzymzubereitung für Wasch- und Reinigungsmittel |
DE4344215A1 (de) * | 1993-12-23 | 1995-06-29 | Cognis Bio Umwelt | Silberkorrosionsschutzmittelhaltige Enzymzubereitung |
FI96090C (fi) * | 1994-01-21 | 1996-05-10 | Valio Oy | Menetelmä heraproteiinien fraktioimiseksi |
DE19615776A1 (de) * | 1996-04-20 | 1997-10-23 | Henkel Kgaa | Löslichkeitsverbessertes Enzymgranulat |
DE19651446A1 (de) * | 1996-12-11 | 1998-06-18 | Henkel Kgaa | Umhüllte Enzymzubereitung mit verbesserter Löslichkeit |
DE69817918T2 (de) * | 1997-04-09 | 2004-07-22 | Rohm And Haas Co. | Entfärbung von Zuckersirup mit einem funktionalisierten Adsorbens enthaltend ein hochvernetztes makroporöses Styren-Copolymer |
DE19956239A1 (de) * | 1999-11-23 | 2001-05-31 | Henkel Kgaa | Verfahren zur Gewinnung von biotechnologischen Wertstoffen |
RU2003105683A (ru) * | 2000-07-28 | 2004-08-20 | Хенкель Кгаа (De) | Новый амилолитический фермент из bacillus sp.а7-7(dsm12368), а также моющее и чистящее средство с этим новым амилолитическим ферментом |
ATE373716T1 (de) * | 2000-11-28 | 2007-10-15 | Henkel Kgaa | Cyclodextrin -glucanotransferase(cg tase) aus bacillus agaradherens(dsm 9948)sowie wasch-und reinigungsmittel mit dieser neuen cyclodextrin- glucanotransferase |
DE10121463A1 (de) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
DE10131441A1 (de) * | 2001-06-29 | 2003-01-30 | Henkel Kgaa | Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen |
DE10138753B4 (de) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen |
DE10153792A1 (de) * | 2001-10-31 | 2003-05-22 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
DE10162728A1 (de) * | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
DE10162727A1 (de) * | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
DE10163748A1 (de) * | 2001-12-21 | 2003-07-17 | Henkel Kgaa | Neue Glykosylhydrolasen |
DE10163883A1 (de) * | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14390) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
DE10163884A1 (de) * | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
DE102004048590A1 (de) * | 2004-04-27 | 2005-11-24 | Henkel Kgaa | Reinigungsmittel mit Klarspül-Sulfopolymer und einer speziellen α-Amylase |
DE102004048591A1 (de) * | 2004-04-27 | 2005-11-24 | Henkel Kgaa | Reinigungsmittel mit Klarspültensid und einer speziellen α-Amylase |
-
2003
- 2003-01-31 DE DE10304066A patent/DE10304066B4/de not_active Expired - Lifetime
-
2004
- 2004-01-23 WO PCT/EP2004/000551 patent/WO2004067557A1/de active Application Filing
- 2004-01-23 EP EP04704587.7A patent/EP1587824B1/de not_active Expired - Lifetime
- 2004-01-23 CN CNB2004800031362A patent/CN100528895C/zh not_active Expired - Fee Related
- 2004-01-23 ES ES04704587.7T patent/ES2533001T3/es not_active Expired - Lifetime
- 2004-01-23 DK DK04704587.7T patent/DK1587824T3/en active
-
2005
- 2005-08-01 US US11/194,333 patent/US20050266542A1/en not_active Abandoned
-
2006
- 2006-05-16 HK HK06105649.8A patent/HK1085747A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ES2533001T3 (es) | 2015-04-06 |
EP1587824A1 (de) | 2005-10-26 |
DK1587824T3 (en) | 2015-03-23 |
DE10304066B4 (de) | 2007-01-18 |
CN1745093A (zh) | 2006-03-08 |
HK1085747A1 (en) | 2006-09-01 |
DE10304066A1 (de) | 2004-08-26 |
US20050266542A1 (en) | 2005-12-01 |
EP1587824B1 (de) | 2014-12-31 |
WO2004067557A1 (de) | 2004-08-12 |
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