CN100528176C - Chinese traditional medicinal composition for treating acne - Google Patents

Chinese traditional medicinal composition for treating acne Download PDF

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CN100528176C
CN100528176C CNB2003101179947A CN200310117994A CN100528176C CN 100528176 C CN100528176 C CN 100528176C CN B2003101179947 A CNB2003101179947 A CN B2003101179947A CN 200310117994 A CN200310117994 A CN 200310117994A CN 100528176 C CN100528176 C CN 100528176C
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medicine
radix
rhizoma
extract
herba
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CN1621082A (en
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瞿幸
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Henan Lingrui Pharmaceutical Co ltd
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Qihuang Pharmaceutical Investment & Research Co Ltd
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Abstract

The acne treat medicine composition contains the extractive of medicine material for clearing away heat, the extractive of medicine material for promoting blood circulation to disperse blood clots, the extractive of medicine material for promoting diuresis and the extractive of medicine material for regulating vital energy. It contains no medicine component for expelling surficial evils and medicine component for nourishing blood.

Description

The Chinese medicine composition of treatment acne
Technical field
The present invention relates to a kind of Chinese medicine composition for the treatment of acne.Concrete, the present invention relates to contain the drug extract of heat clearing away medicine, blood circulation promoting and blood stasis dispelling medicine, promoting diuresis to eliminate damp pathogen medicine, the medicine of regulating the flow of vital energy, do not contain the Chinese medicine composition of the treatment acne of separating harmony in the exterior hematonic thing.In addition, the invention still further relates to the Chinese medicine extract of the treatment acne that contains baicalin and TANSHINONES.
Background technology
Acne (acne) is the common frdquently encountered disease of department of dermatologry, has another name called acne, acne vulgaris (acnevulgaris) etc.According to external, its adolescence sickness rate is more than 90%, and domestic the mid-90 reports that this disease has become one of commonly encountered diseases frequently-occurring disease that department of dermatologry pays close attention to the most, account for the daily outpatient service amount of department of dermatologry about 13% and increase with annual 1% speed.
The pathogeny complexity of acne is mainly androgen secretion increasing, sebaceous gland hyperfunctioning, hypersteatosis, hair follicle sebum conduit dyskeratosis, pilosebaceous unit microbial action and inflammatory reaction etc.Though acne does not have life danger usually, the pruritus when falling ill, pain etc. cause suffering, and the blackhead that generates, acne, pimple etc. all influence attractive in appearancely, and the quality of life to people impacts in varying degrees.
Doctor trained in Western medicine adopts pharmacotherapy and physiotherapy more at present.Pharmacotherapy is mainly oral and external medication, and oral drugs mainly contain medicines such as antibiotic, isotretinoin, estrogen, adrenocortical hormone; External mainly contains antibiotic, retinoic acid, benzoyl peroxide, estrogen, adrenocortical hormone, zinc salt etc.; Physiotherapy mainly contains ultraviolet, X ray, freezing, laser etc.These therapies can obtain certain curative effect but have weak points such as relapse rate height, zest be big.
The Chinese patent medicine of treatment acne existing as: little leaf deervetch herb acne removing ball (forming) by Fructus Gardeniae, Herba Menthae, Radix Et Rhizoma Rhei, Flos Lonicerae, Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Platycodonis, Radix Glycyrrhizae; Dermopathic blood poison pill (forming) by 39 herbal medicines such as Radix Rubiae, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Radix Rehmanniae Preparata, Herba Schizonepetae, Periostracum Serpentis, the Fructus Kochiae, Fructus Xanthii, the Radix Angelicae Dahuricae, Radix Saposhnikoviae, Herba Spirodelae, Periostracum Cicadae, Fructus Arctii, Radix Platycodonis, Semen Armeniacae Amarum, Poria, Radix Et Rhizoma Rhei, Cortex Phellodendri, Rhizoma Imperatae, Radix Paeoniae Rubra, Radix Rehmanniae, Cortex Moutan, Radix Arnebiae (Radix Lithospermi), Fructus Forsythiae, Flos Lonicerae, Rhizoma Smilacis Glabrae, Cortex Dictamni, Caulis Lonicerae, Rhizoma Bolbostematis, Radix Semiaquilegiae, Herba Leonuri, Spina Gleditsiae, Semen Persicae, Rhizoma Chuanxiong, Cortex cercis chinensis, Caulis Spatholobi, Flos Carthami, Radix Glycyrrhizaes; QINGRE ANCHUANG PIAN (forming) by Flos Lonicerae, extractum rhei, creat extract, Calculus Bovis, extract of taraxacum, Radix Sophorae Tonkinensis extractum, Margarita layer powder, Fructus Gardeniae extractum, Radix Glycyrrhizae; DANGGUI KUSHEN WAN (forming) etc. by Radix Angelicae Sinensis, Radix Sophorae Flavescentis.
Existing these medicines are many based on the heat-clearing and toxic substances removing medicine, contain the medicine etc. that induces sweat, enriches blood more than in addition.In view of acne mechanism complexity, paathogenic factor are many, simple heat-clearing and toxic substances removing is difficult to obtain better curative effect.The induce sweat reason of medicine of employing is according to medical science ancient books and records (" General Treatise on the Cause and Symptoms of Diseases acne vulgaris time ", " Yizong Jinjian rosacea caused by lung-wind " etc.), thinks that acne mainly is because lung-heat causes.But in recent years studies show that the cause of disease is not limited to lung-heat, therefore treatment is based on this attended to one thing and lose sight of another unavoidably.And we think acne no thanks to blood deficiency therefore cause that enriching blood probably is difficult to play therapeutical effect.
Existing medicine, though some also has other auxiliary elements to cooperate, flavour of a drug are various, the interaction between the medicine is difficult to grasp.In addition, present Chinese patent medicine majority is will to make after the medicine simple process merely, and dose is big, and interdependence was relatively poor when patient took.
Multiple factor such as microorganism is relevant in studies show that of pathology in recent years etc., the generation of acne and endocrine, sebum and hair follicle.Relatively the explanation of Gong Rening is, because the androgen level increases, stimulate the sebaceous hyperplasia hypertrophy, sebum secretion increases, promote a large amount of breedings of the propionibacterium (Propiobacterium) that exists in the cortex hair follicle and other resident bacterium (as staphylococcus aureus etc.), the generation free fatty that reduces fat causes parafollicular inflammatory reaction etc., causes generation and other related symptoms of acne.Through research, the heat clearing away medicine has fungistatic effect to propionibacterium acnes, staphylococcus aureus etc.
In addition, by the Hemorheology Study to patients with acne, find that its blood viscosity is generally higher, the hint blood viscosity increases and may fall ill relevant with acne.This result provides certain foundation (Wang Baofang etc., Tianjin College of Traditional Chinese Medicine journal, 1995, (2): 41) for adopting activating blood and removing stasis drug treatment acne.
Summary of the invention
The inventor etc. find by practice, by adopting based on heat clearing away medicine, blood circulation promoting and blood stasis dispelling medicine, the compositions of be aided with the promoting diuresis to eliminate damp pathogen medicine, the extract of Chinese medicine such as the medicine of regulating the flow of vital energy being formed can be eliminated inflammatory reactions such as hairy cyst that acne causes, suppress acne bacteria, and is effective to the treatment acne.
In addition, we also find, contain the pharmaceutical composition of baicalin and TANSHINONES, and are effective to the treatment acne.
Thus, the present invention relates to:
1. the pharmaceutical composition of treatment acne wherein contains mainly and is made up of heat clearing away medicine and blood circulation promoting and blood stasis dispelling medicine, and the extract of the Chinese medicine composition of the promoting diuresis to eliminate damp pathogen medicine and the medicine of regulating the flow of vital energy, and does not contain the induce sweat medicine and/or the medicine of enriching blood in the Chinese medicine composition simultaneously.
2. according to above-mentioned 1 pharmaceutical composition, wherein the heat clearing away medicine is selected from heat clearing and damp drying medicine and heat-clearing and toxic substances removing medicine, and the heat clearing and damp drying medicine is more than the heat-clearing and toxic substances removing medicine.
3. according to 1 or 2 pharmaceutical composition, wherein the heat clearing away medicine is selected from Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri, Radix Gentianae, Radix Sophorae Flavescentis, Sophora Alopecuroides L., Radix et Rhizoma Thalictri, Ramulus Et Folium Picrasmae, heat clearing and damp drying medicine and/or Flos Loniceraes such as Caulis Mahoniae, Caulis Lonicerae, Fructus Forsythiae, Herba Taraxaci, Folium Isatidis, Radix Isatidis, Indigo Naturalis, Herba Andrographis, Calculus Bovis, Rhizoma Paridis, Rhizoma Bistortae, Herba Lobeliae Chinensis, Herba Sedi, Rhizoma Smilacis Glabrae, Herba Houttuyniae, Rhizoma Belamcandae, Radix Sophorae Tonkinensis, BEIDOUGEN, Lasiosphaera Seu Calvatia, Calyx Seu Fructus Physalis, Herba Portulacae, the Radix Pulsatillae, Cortex Fraxini, Fructus Bruceae, Caulis Sargentodoxae, Herba Patriniae, Herba Hedyotidis Diffusae, Radix Ampelopsis, Cortex Dictamni, Radix Rhapontici, Pseudobulbus Cremastrae Seu Pleiones, Flos Chrysanthemi Indici, Herba Potentillae Chinensis, Rhizoma Bolbostematis, Radix Semiaquilegiae, Herba Cichorii, Ilex purpurea Hassk.[I.chinensis Sims, heat-clearing and toxic substances removing medicines such as Herba Senecionis Scandentis; The blood circulation promoting and blood stasis dispelling medicine is selected from Rhizoma Chuanxiong, Olibanum, Myrrha, Rhizoma Corydalis, Radix Curcumae, Rhizoma Curcumae Longae, Rhizoma Curcumae, rhizoma sparganic, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Leonuri, Fructus Leonuri, Caulis Spatholobi, Semen Persicae, Flos Carthami, Stigma Croci, Oletum Trogopterori, Radix Achyranthis Bidentatae, Squama Manis, Spina Gleditsiae, Semen Vaccariae, Eupolyphaga Seu Steleophaga, Hirudo, Herba Lycopi, Flos Rosae Chinensis, Flos Campsis, Herba Artemisiae Anomalae, Lignum Sappan, Resina Toxicodendri, Herba Selaginellae, Fructus Polygoni Orientalis, Retinervus Luffae Fructus, Cortex cercis chinensis, Rhizoma Corydalis Decumbentis etc.; The promoting diuresis to eliminate damp pathogen medicine is selected from that Poria, Polyporus, Rhizoma Alismatis, Semen Coicis, Semen Plantaginis, Herba Plantaginis, Talcum, Caulis Akebiae, Medulla Tetrapanacis, Herba Lysimachiae, Spora Lygodii, Folium Pyrrosiae, Herba Artemisiae Scopariae, Herba Swertiae Mileensis, Exocarpium Benincasae, Semen Benincasae, Pericarpium Arecae, Semen Phaseoli, Herba Dianthi, Medulla Junci, the Fructus Kochiae, Herba Adonidis, calabash, EXOCARPIUM CITRULLI, corn are continuous, Fructus rhamni (Rhamnus davurica Pall.), Herba Capsellae etc.; The medicine of regulating the flow of vital energy is selected from Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Fructus Citri, Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus), the Radix Aucklandiae, Herba Aristolochiae, Rhizoma Cyperi, the Radix Linderae, Fructus Toosendan, Semen Canavaliae etc.
5. according to above-mentioned each pharmaceutical composition, wherein Chinese medicine is selected from Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Artemisiae Scopariae and Pericarpium Citri Reticulatae.
6. according to above-mentioned each compositions, it is characterized in that, by Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Artemisiae Scopariae and Pericarpium Citri Reticulatae with 15: 10: 10: 12: 12: 10: the extract that 15: 10 ratio obtains was formed.
7. according to above-mentioned each compositions, it is characterized in that this pharmaceutical composition is made powder, tablet, piece agent or granule, medicinal tea or capsule.
8. according to above-mentioned 7 compositions, it is characterized in that said composition is made capsule.
9. the pharmaceutical composition of treatment acne contains baicalin and TANSHINONES.
10. baicalin and the TANSHINONES application in the medicine of treatment acne.
The specific embodiment
Below in the narration, unless otherwise indicated, % is meant weight percentage.
" mainly containing " among the present invention is meant content in the total amount of raw medicinal material, for surpassing 50% amount, preferably more than 60%, more preferably in the amount more than 70%.But should be noted in the discussion above that " mainly " cannot not contain other medicines in not representing to form among the present invention, therefore, consider the existence of other composition, preferably be no more than 90%, more preferably no more than 80%.
We find by practice, by adopting based on heat clearing away medicine, blood circulation promoting and blood stasis dispelling medicine, the compositions that is aided with the extract composition of Chinese medicines such as diuretic oozes warm medicine, the medicine of regulating the flow of vital energy can be eliminated inflammatory reactions such as hairy cyst, inhibition acne bacteria that acne causes, and is effective to the treatment acne.
Different with understanding in the past, do not contain the induce sweat medicine and/or the medicine of enriching blood in the Chinese medicine composition of the present invention.
We also find, if serve as main changing into heat-clearing and toxic substances removing medicine in the past based on the heat clearing and damp drying medicine with the heat clearing away medicine, and the better effects if of treatment acne.
Specifically; the heat clearing away medicine can be selected from heat clearing away medicine such as Radix Scutellariae commonly used; Rhizoma Coptidis; Cortex Phellodendri; Radix Gentianae; Radix Sophorae Flavescentis; Sophora Alopecuroides L.; Radix et Rhizoma Thalictri; Ramulus Et Folium Picrasmae; heat clearing and damp drying medicines such as Caulis Mahoniae, and Flos Lonicerae; Caulis Lonicerae; Fructus Forsythiae; Herba Taraxaci; Folium Isatidis; Radix Isatidis; Indigo Naturalis; Herba Andrographis; Calculus Bovis; Rhizoma Paridis; Rhizoma Bistortae; Herba Lobeliae Chinensis; Herba Sedi; Rhizoma Smilacis Glabrae; Herba Houttuyniae; Rhizoma Belamcandae; Radix Sophorae Tonkinensis; BEIDOUGEN; Lasiosphaera Seu Calvatia; Calyx Seu Fructus Physalis; Herba Portulacae; the Radix Pulsatillae; Cortex Fraxini; Fructus Bruceae; Caulis Sargentodoxae; Herba Patriniae; Herba Hedyotidis Diffusae; Radix Ampelopsis; Cortex Dictamni; Radix Rhapontici; Pseudobulbus Cremastrae Seu Pleiones; Flos Chrysanthemi Indici; Herba Potentillae Chinensis; Rhizoma Bolbostematis; Radix Semiaquilegiae; Herba Cichorii; Ilex purpurea Hassk.[I.chinensis Sims; heat-clearing and toxic substances removing medicines such as Herba Senecionis Scandentis.
According to our research, though be not limited to certain specific theory, find that the heat clearing and damp drying medicine plays topmost effect in remedy of acne, the heat-clearing and toxic substances removing medicine also is indispensable simultaneously.On the amount of using, the heat clearing and damp drying medicine should be more than the heat-clearing and toxic substances removing medicine, and this is opposite with in the past conventional way.In the past in the medicine of treatment acne, usually the heat-clearing and toxic substances removing medicine will be more than the heat clearing and damp drying medicine, even does not use the heat clearing and damp drying medicine sometimes.
In the heat clearing and damp drying medicine, one or more in preferred Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri, Radix Gentianae, the Radix Sophorae Flavescentis etc.
Radix Scutellariae is the dry root of labiate Radix Scutellariae Scutellaria baicalensis Geoogi, has the effect of heat clearing and damp drying, eliminating fire and detoxication.Its effective ingredient is mainly flavonoid and flavanone compounds, as baicalin, baicalin, wogonoside, wogonin, chrysin, oroxylin etc.Wherein baicalin and wogonin are respectively the glycoside units of baicalin and wogonoside, flavone compounds such as baicalin, baicalin, wogonoside, wogonin general designation baicalin compounds.
Rhizoma Coptidis is the dry rhizome of the generic plant of also using as Rhizoma Coptidis usually of ranunculaceae plant Rhizoma Coptidis Coptis chinensis Franch, Coptis deltoidea C.Y.Cheng et Hsiao C.deltoidea C.Y.Cheng et Hsiao, Coptis Teeta Wall C.teeda Wall. and other.Effect with heat clearing and damp drying, eliminating fire and detoxication.Wherein main pharmacological component is an isoquinoline alkaloid, as berberine, coptisine, 13-methyl-.psi.-coptisine., palmatine, jateorhizine etc.Pharmaceutical research shows that this Alkaloid not only has antibiotic, antiviral activity, and also has antiinflammatory action.
Cortex Phellodendri is the dry bark of rutaceae wampee Phellodendron chinense Rupr. or Cortex Phellodendri P.amurense Schneid..Effect with heat clearing and damp drying, eliminating fire and detoxication.Wherein main pharmacological component is an isoquinoline alkaloid, except that berberine, palmatine etc., also has phellodendrine etc.
Radix Gentianae is the dry root and rhizome of gentianaceae plant bar leaf Radix Gentianae Gentiana manshurica Kitag., Radix Gentianae G.scabra Bge, three flower Radix Gentianae G.triflora Pall. or Gentiana rigescens Franch G.regescensFranch. etc.Effect with heat clearing and damp drying, clearing away liver-fire.Wherein main pharmaceutical component is ter penoidses such as gentiopicrin, swertiamarin.
Radix Sophorae Flavescentis is the dry root of leguminous plant Radix Sophorae Flavescentis Sophora flavescens Ait..Effect with heat clearing and damp drying, wherein main pharmacological component is alkaloids such as matrine, oxymatrine.This Alkaloid has good anti-allergic effects.
We find that from active and adaptable angle, the heat clearing and damp drying medicine is Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis more preferably.
The preferred Radix Et Rhizoma Rhei of heat-clearing and toxic substances removing medicine, Flos Lonicerae, Fructus Forsythiae, Rhizoma Smilacis Glabrae, Herba Taraxaci etc.
Radix Et Rhizoma Rhei is the dry root and rhizome of polygonum rheum palmatum Rheum palmatum L., Rheum tanguticum R.tanguticum Maxim.ex Balf. or Rheum officinale R.officinale Baill..Its effect is heat-clearing and toxic substances removing, rush down down, blood circulation promoting and blood stasis dispelling etc.Wherein main effective ingredient is anthraquinone analog compound and glycoside thereof.
Flos Lonicerae is that caprifoliaceae plant is born eastern Lonicera japonica Thunb., red body is born the dry flower of eastern L.hypoglauca Miq., Flos Lonicerae L.confusa DC or hair style Radix Ophiopogonis L.dasystyla Rehd. or the flower that band is just opened.Its effectiveness has the effect of heat-clearing and toxic substances removing, expelling wind and cold pathogens.Wherein contain multiple terpenoid volatile oil component, have good effect such as antibiotic grade.
Fructus Forsythiae is the dry fruit of oleaceae plant Fructus Forsythiae Forsythia suspendsa (Thunb.) Vahl.Its drug effect is a heat-clearing and toxic substances removing, and eliminating carbuncle looses and saves.Wherein contain belulinic acid Betulinic acid, forsythin, pinoresinol, Forsythinol glycoside etc.These chemical compounds have good effect such as antibiotic grade.
Rhizoma Smilacis Glabrae is the dry rhizome of liliaceous plant smilacis glabra Smilax glabra Roxb., and its effect wherein contains the smilax saponin compounds for detoxifcation, dehumidifying, therefore also has antiphlogistic effect.
Herba Taraxaci is feverfew Herba Taraxaci Taraxacum mongolicum Hand.-Mazz., alkali ground Herba Taraxaci T.sinicum Kitag. or the dry herb that belongs to other plant together.Its drug effect is heat-clearing and toxic substances removing, dampness removing etc.Main component contains terpenoid, volatilization wet goods, not only has antibacterial action, also can the excitating organism immunologic function.
The heat-clearing and toxic substances removing medicine is Fructus Forsythiae most preferably.
The heat clearing away medicine, comprise heat clearing and damp drying medicine and heat-clearing and toxic substances removing medicine, preferably account for more than the 40 weight % of composition material medicine, more preferably more than 50 weight %, but the heat clearing away medicine is not The more the better, consider the interaction of medicine, the amount of heat clearing away medicine preferably is no more than 70 weight % of total amount, is preferably in below the 60 weight %.
Between heat clearing and damp drying and heat-clearing and toxic substances removing medicine, as mentioned above, the heat clearing and damp drying medicine should be more than the heat-clearing and toxic substances removing medicine, and preferred content is not higher than 4: 1 than more than 2: 1.The ratio of the medicine of heat clearing and damp drying medicine and heat-clearing and toxic substances removing is most preferably about 3: 1.
As mentioned above, patients with acne much has the viscid body constitution of blood, and for this reason, we add the blood circulation promoting and blood stasis dispelling medicine in compositions, and by blood circulation promoting, the viscosity that brings high blood pressure down in the hope of improving acne symptom, has obtained success.
The blood circulation promoting and blood stasis dispelling medicine is optional from Rhizoma Chuanxiong, Olibanum, Myrrha, Rhizoma Corydalis, Radix Curcumae, Rhizoma Curcumae Longae, Rhizoma Curcumae, rhizoma sparganic, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Leonuri, Fructus Leonuri, Caulis Spatholobi, Semen Persicae, Flos Carthami, Stigma Croci, Oletum Trogopterori, Radix Achyranthis Bidentatae, Squama Manis, Spina Gleditsiae, Semen Vaccariae, Eupolyphaga Seu Steleophaga, Hirudo, Herba Lycopi, Flos Rosae Chinensis, Flos Campsis, Herba Artemisiae Anomalae, Lignum Sappan, Resina Toxicodendri, Herba Selaginellae, Fructus Polygoni Orientalis, Retinervus Luffae Fructus, Cortex cercis chinensis, Rhizoma Corydalis Decumbentis etc.Preferred Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Leonuri, Spina Gleditsiae, Semen Persicae, Flos Carthami, Cortex cercis chinensis, Caulis Spatholobi.
Rhizoma Chuanxiong is the dry rhizome of samphire Rhizoma Chuanxiong Ligusticum chuanxiong Hort., and its drug effect is blood-activating and qi-promoting, wind-expelling pain-stopping etc.Wherein contain volatile oil, lactone, alkaloid and phenols etc.
Radix Salviae Miltiorrhizae is the dry root and rhizome of labiate Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bge..Its drug effect is blood circulation promoting and blood stasis dispelling, removing heat from blood eliminating carbuncle etc.Wherein mainly contain furo phenanthrenequione constituents, as tanshinone, cryptotanshinone etc.Can microcirculation improvement, enhance immunity, have certain antibacterial action simultaneously.
Rhizoma Polygoni Cuspidati is polygonaceae plant Rhizoma Polygoni Cuspidati Polygonum cuspidatum Sieb.et Zucc. rhizome and root.Wherein contain anthraquinone class such as emodin and glycoside thereof and and some diphenylethylene compounds such as Polydatin etc.
Herba Leonuri is the dry aerial parts of labiate Herba Leonuri Leonurus heterophyllus Sweet.Its drug effect is blood circulation promoting and blood stasis dispelling, inducing diuresis to remove edema etc.Wherein contain alkaloids such as leonurine, stachydrine.
Spina Gleditsiae is the dry Spina jujubae of leguminous plant Fructus Gleditsia Gleditsia sinensis Lam..Can the detoxification evacuation of pus, the eliminating carbuncle of invigorating blood circulation.
Semen Persicae is the dry mature seed of rosaceous plant peach Prunus persica (Linn) Batsch. or mountain peach P.davidiana (Carr.) Franch.Its drug effect is a blood circulation promoting and blood stasis dispelling etc.Wherein contain amygdalin etc.
Flos Carthami is the dried floral of feverfew Flos Carthami Carthamus tinctorius L..Can invigorate blood circulation, detumescence, pain relieving.Wherein contain flavone compound Flos Carthami glycoside etc., and the Flos Carthami polysaccharide etc.Can antiplatelet, anticoagulation, promote lymphocyte transformation etc.
Cortex cercis chinensis is the dry root bark of the long stalk of Magnoliacea plant Fructus Schisandrae Sphenantherae Kadsura longipedunculataFinet et Gagnep.Its drug effect is promoting blood circulation to restore menstrual flow, removing toxic substances and promoting subsidence of swelling etc.
Caulis Spatholobi is the dry rattan of leguminous plant spatholobus suberectus Spatholobus suberectus Dunn.Its drug effect is that promoting the circulation of blood is enriched blood etc.
The blood circulation promoting and blood stasis dispelling medicine is Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Cortex cercis chinensis etc. more preferably.Most preferably Radix Salviae Miltiorrhizae and Rhizoma Polygoni Cuspidati.
According to inventor's etc. research, in the compositions of the present invention, the consumption of blood circulation promoting and blood stasis dispelling medicine, in the total amount of the medical material of forming compositions, should consider other composition in the compositions more than 10%, its content preferably is no more than 40%, and preferred about 20～30%.Simultaneously, the content of blood circulation promoting and blood stasis dispelling medicine should be less than the heat clearing away medicine, is preferably in more than 1/4 of heat clearing away medicine, below 1/2.The content of blood circulation promoting and blood stasis dispelling medicine is less than 1/4 of heat clearing away medicine, and it is invigorated blood circulation, the effect of blood circulation promoting might be brought into play insufficiently, and the effect of heat clearing away medicine also is difficult to performance.If surpassed 1/2, the heat clearing away content of medicines, so drug effect also might be able to not be given full play to.
Discoveries such as the inventor, in compositions of the present invention, though do not rely on any existing explanation, except heat clearing away medicine and blood circulation promoting and blood stasis dispelling medicine, assistant can further be brought into play the remedy of acne effect with the promoting diuresis to eliminate damp pathogen medicine and/or the medicine of regulating the flow of vital energy.
The promoting diuresis to eliminate damp pathogen medicine is selected from that Poria, Polyporus, Rhizoma Alismatis, Semen Coicis, Semen Plantaginis, Herba Plantaginis, Talcum, Caulis Akebiae, Medulla Tetrapanacis, Herba Lysimachiae, Spora Lygodii, Folium Pyrrosiae, Herba Artemisiae Scopariae, Herba Swertiae Mileensis, Exocarpium Benincasae, Semen Benincasae, Pericarpium Arecae, Semen Phaseoli, Herba Dianthi, Medulla Junci, the Fructus Kochiae, Herba Adonidis, calabash, EXOCARPIUM CITRULLI, corn are continuous, Fructus rhamni (Rhamnus davurica Pall.), Herba Capsellae etc.Preferred Poria, the pig tinkling of pieces of jade, Herba Artemisiae Scopariae, Exocarpium Benincasae, EXOCARPIUM CITRULLI etc.
Poria is the dry sclerotia of Polyporaceae fungus Poria Poria cocos (Schw.) Wolf.Can promoting diuresis to eliminate damp pathogen, invigorating spleen and reinforcing stomach etc.Contain polysaccharides such as triterpene carboxylic acids such as pachymic acid and pachyman.
Polyporus is the dry sclerotia of Polyporaceae fungus Polyporus Polyporus umbellatus (Pers.) Fr..Can promoting diuresis to eliminate damp pathogen etc.Contain polysaccharides such as Polyporus polysaccharide etc.
Herba Artemisiae Scopariae is the dry seedling of feverfew Herba Artemisiae Scopariae Artemisia capillaries Thunb., in addition, also can use usually also as what Herba Artemisiae Scopariae was used and belong to other plant etc. together.Can clearing away heat-damp and promoting diuresis.Contain volatile oil such as capillin etc., flavones ingredient capillarisin etc.Its water extract can suppress various bacteria, and volatile oil can some pathomycete of strong inhibition.
Exocarpium Benincasae is the dry outer peel of cucurbitaceous plant Fructus Benincasae Benincasa hispida (Thunb.) Cogn..Effect is an inducing diuresis to remove edema etc.
EXOCARPIUM CITRULLI is the peel of cucurbitaceous plant Citrullus vulgaris Citrullus vulgaris Schrad..Effect is that clearing away summer-heat is analgesic, quenches the thirst etc.
The medicine of regulating the flow of vital energy is selected from Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Fructus Citri, Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus), the Radix Aucklandiae, Herba Aristolochiae, Rhizoma Cyperi, the Radix Linderae, Fructus Toosendan, Semen Canavaliae etc.Preferred Pericarpium Citri Reticulatae.
Pericarpium Citri Reticulatae is the dry mature skin of rutaceae orange Citrus reticulata Blanco.Can regulate the flow of vital energy transfer in, drying dampness to eliminate phlegm etc.Contain volatile oil and flavonoid glycosides etc.
The promoting diuresis to eliminate damp pathogen medicine and/or the content of medicines of regulating the flow of vital energy in the total amount of the medical material of forming compositions, should be considered other composition in the compositions more than 10%, and its content preferably is no more than 40%, preferred about 20～30%.Contain the promoting diuresis to eliminate damp pathogen medicine at the same time and the situation of medicine of regulating the flow of vital energy under, the content of promoting diuresis to eliminate damp pathogen medicine with regulate the flow of vital energy medicine can be roughly the same, perhaps be slightly more than the latter, but preferably be no more than 2 times, be preferably in about 1.5 times.
Comprise and contain the heat clearing and damp drying medicine that is selected from Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis preferred a composition of the present invention, be selected from the heat-clearing and toxic substances removing medicine of Fructus Forsythiae, be selected from the activating blood circulation to dissipate blood stasis medicine of Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, and the promoting diuresis to eliminate damp pathogen medicine and the medicine of regulating the flow of vital energy that is selected from Pericarpium Citri Reticulatae that are selected from Herba Artemisiae Scopariae.
Especially, when Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Artemisiae Scopariae and Pericarpium Citri Reticulatae with 15: 10: 10: 12: 12: 10: when 15: 10 ratio was formed compositions, its drug effect can better be brought into play.
The consumption of the present composition, according to the crude drug meter, every day consumption between 10g (2 money)～100g (2 liang), general consumption is about 15g (3 money)～75g (1 two halves), preferred 20g (4 money)～50g (1 liang) is preferably in about 25g (half two) (according to circumstances can ± about 5g).
Because remedy of acne needs the long period, when selecting dosage form, need to select volume little, the dosage form of carrying taking convenience.Conventional fried preparation is inconvenient to carry and take, and therefore is difficult to satisfy above-mentioned requirements.So from the angle of taking medicine for a long time, preferred solid preparation.
As solid preparation, powder, capsule, tablet, medicinal tea, piece agent or granule etc. are arranged.If the present invention adopts solid preparation,, should make extractum earlier according to above-mentioned narration.During the refabrication granule, can add a certain amount of adjuvant and promote pelletize and extractum dissolving, can select according to the general knowledge of this area as the adjuvant of granule.During the preparation compressed tablets, concrete technology for example is mixed into a certain amount of adjuvant etc. with extractum, adds system soft material, pelletize simultaneously, beats sheet.From the preparation angle, capsule can directly or only be enclosed extractum in the gelatine capsule by simple process, and technology is simple.Simultaneously owing to directly can discharge ingredient after the capsule dissolves, the medicine stripping is good, the onset piece, and curative effect is played stably, and taking convenience, volume are little, so capsule is most preferred.Simultaneously,,, realize treatment, also meet theory of Chinese medical science about supporting outer thinking with interior to external morbidity by regulating the equilibrium between yin and yang of body as oral preparations.
Certainly, guaranteeing taking convenience, under the prerequisite of taking easily, other oral or parenteral drug-delivery preparation such as oral liquid, medicated wine and external preparation such as liniment or ointment, plaster, paste etc. also can adopt.Those skilled in the art can according to concrete composition and the extraction process that adopts determine that this variation all belongs to scope of the present invention.
Below introduce preparation process of the present invention.
Belong to general knowledge well known in the art because extract the technology of extractum, do not intend this is done too much restriction among the present invention.Should illustrate that the effective ingredient of the medical material that contains in the compositions has is water miscible, there also have to be fat-soluble, contains water soluble ingredient and liposoluble constituent when having.For containing fat-soluble or containing the medical material of water solublity and liposoluble constituent simultaneously, should take at first to extract liposoluble constituent with the mode of alcohol extraction or other organic solvent such as extractions such as ether, petroleum ether.For the medical material that contains water soluble ingredient, under the situation that does not cause drug interaction, can extract by blending water.For the medical material that contains water solublity and liposoluble constituent simultaneously, after extracting liposoluble constituent, also can use water extraction together.For the medical material that contains the volatile oil effective ingredient, should avoid the loss of volatile oil in leaching process.For example can extract earlier after the volatile oil, again that its enclose is standby in the carriers such as cyclodextrin.For the heat-labile medical material of effective ingredient, can be according to the conventional way of the field of Chinese medicines, extract effective ingredient with its pulverizing or with low temperature method after, be mixed in the extractum.
More than extract and to adopt method well known in the art, can take methods such as solvent impregnated, percolation, reflux, extract, when for example extracting liposoluble constituent.Solvent is lower aliphatic alcohols such as methanol, ethanol for example, low boiling volatile solvent such as ether, petroleum ether, plant wet goods.Also can utilize and extract such as methods such as supercritical fluid extractions.If fat-soluble solvent is water soluble also, also can adopt solvent/water mixture such as alcohol/hydrate etc.When extracting water soluble ingredient, can take methods such as water logging, decoction, ultrasound wave or microwave extraction, also said method can be used in combination.
Should illustrate, for Chinese medicine, though extractum can be further purified with further decrement, but according to our practice, adopt lower common water extract of purity and alcohol-extracted extract etc., when guaranteeing curative effect, can effectively reduce production costs, from being favourable economically.
Also should be noted that at this, we think from the extract of Chinese medicine composition of the present invention, from Radix Scutellariae, extract with the baicalin compounds be the flavone component (below be referred to as the baicalin chemical compound) of representative and from Radix Salviae Miltiorrhizae, extract with Tanshinone I, II, the cryptotanshinone class is furo phenanthrenequione constituents (below be referred to as the TANSHINONES chemical compound) role maximum in the treatment acne of representative, so the present invention also comprises and contains the application in the treatment acne of baicalin chemical compound and TANSHINONES compound compositions and above-mentioned two compounds (comprising wherein contained monomeric compound).
Below enumerate example and specify extraction and preparation method.
For for simplicity, the compositions by following composition describes, but the present composition is not subjected to the restriction of following composition.On introduction of the present invention basis, according to the general knowledge of this area, can carry out other combination, these combinations and variation thereof all belong to scope of the present invention.
[embodiment 1]
The heat clearing and damp drying medicine selects for use Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, heat-clearing and toxic substances removing medicine to select for use Fructus Forsythiae, blood circulation promoting and blood stasis dispelling medicine to select that Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, promoting diuresis to eliminate damp pathogen medicine are selected Herba Artemisiae Scopariae for use, the choice of drug Pericarpium Citri Reticulatae of regulating the flow of vital energy for use.
According to preparation 1000 capsules meters, each composition consumption is as follows:
Radix Scutellariae 300g Rhizoma Coptidis 200g Radix Sophorae Flavescentis 200g Fructus Forsythiae 240g
Radix Salviae Miltiorrhizae 240g Rhizoma Polygoni Cuspidati 200g Herba Artemisiae Scopariae 300g Pericarpium Citri Reticulatae 200g
Above flavour of a drug, Radix Scutellariae decoct with water 3 times, each 2 hours, collecting decoction filters, and filtrate is concentrated between relative density 1.05-1.10, regulate pH value 1.5-2.0,70 ℃ are incubated 30 minutes, left standstill sucking filtration 24 hours, precipitation with washing with alcohol to pH value 5.0-6.5, after the washing of reuse low amounts of water, drying under reduced pressure is ground into fine powder; Rhizoma Coptidis, Radix Sophorae Flavescentis two flavors add 60% alcohol reflux 3 times, and each 1.5 hours, filter, filtrate recycling ethanol, and be condensed into dry extract, be ground into fine powder; Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati two flavors add 90% ethanol extraction 2 times, and each 1.5 hours, filter, medicinal residues are standby, merge ethanol extract, decompression recycling ethanol, and be condensed into dry extract, be ground into fine powder; Pericarpium Citri Reticulatae, Fructus Forsythiae, Herba Artemisiae Scopariae are extracted volatile oil with vapor distillation respectively, and use beta-cyclodextrin inclusion compound; Aqueous solution after distillation device is in addition collected; Medicinal residues and above-mentioned medicinal residues merge, and decoct with water each 1.5 hours 2 times, filter, filtrate and above-mentioned aqueous solution merge, and being concentrated into relative density is 1.10 clear paste, add ethanol and make and contain the alcohol amount and reach 60%, stir evenly, left standstill (2-8 ℃) 24 hours, and filtered filtrate recycling ethanol, and be condensed into dry extract, be ground into fine powder; Above-mentioned fine powder merges, and adds volatile oil beta cyclodextrin inclusion complex and dextrin, micropowder silica gel is an amount of, and mixing is made granule, and drying incapsulates, and makes 1000, that is, content is 450 milligrams in every.
Containing with baicalin, forsythin, hesperidin etc. in the gained capsule is the flavonoid glycosides of representative, with berberine hydrochloride, matrine etc. is the alkaloids of representative, with TANSHINONES, emodin etc. is the anthraquinone class of representative, with danshensu, protocatechualdehyde etc. is the chemical compounds such as phenolic acids of representative, and other contains other material of volatilization wet goods.Above composition can adopt the method for 1 one of Pharmacopoeia of People's Republic of China version in 2000 to measure.
Thin layer chromatography according to the pharmacopeia record is differentiated Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Rhizoma Polygoni Cuspidati, Radix Salviae Miltiorrhizae, Pericarpium Citri Reticulatae, has identified the matrine of Rhizoma Coptidis medical material and berberine hydrochloride, Radix Sophorae Flavescentis, the Tanshinone I I of Radix Salviae Miltiorrhizae A, the emodin of Radix Et Rhizoma Rhei and physcione, Fructus Forsythiae the existence of hesperidin of forsythin, Pericarpium Citri Reticulatae.Simultaneously its moisture, arsenic salt, heavy metal, hygiology etc. are checked and measured, all meet standards of pharmacopoeia.
High performance liquid chromatography according to the pharmacopeia record is measured baicalin, and content is more than 15 milligrams/.
[embodiment 2]
According to embodiment 1 same quadrat method, use and amplify 10 times of amount preparation capsules.
That is, select Radix Scutellariae 3000g for use, get crude extract (dry product, down together) 421 grams; Rhizoma Coptidis and Radix Sophorae Flavescentis 4000g get crude extract 817 grams; Radix Salviae Miltiorrhizae and Rhizoma Polygoni Cuspidati 4400g get crude extract 725 grams; Pericarpium Citri Reticulatae, Fructus Forsythiae and Herba Artemisiae Scopariae 7400g get 9.0 milliliters of volatile oil, use beta-cyclodextrin inclusion compound, get clathrate 52 grams, obtain crude extract 1624 grams in addition.
Above extract (containing clathrate) is totally 3639 grams, and each 430.5 restrains into granule to add dextrin and micropowder silica gel.Gross weight is 4482 grams, and is encapsulated according to 450 milligrams/amount, obtains 9954 capsules.
Every inspection is the same.
[embodiment 3]
According to embodiment 1 same quadrat method, use and amplify 25 times of amount preparation capsules.
That is, select Radix Scutellariae 7500g for use, get crude extract 1121 grams; Rhizoma Coptidis and Radix Sophorae Flavescentis 10000g get crude extract 1936 grams; Radix Salviae Miltiorrhizae and Rhizoma Polygoni Cuspidati 11000g get crude extract 1806 grams; Pericarpium Citri Reticulatae, Fructus Forsythiae and Herba Artemisiae Scopariae 18500g get 21.5 milliliters of volatile oil, use beta-cyclodextrin inclusion compound, get clathrate 136 grams, obtain crude extract 3965 grams in addition.Above extract (containing clathrate) is totally 8964 grams, and each 1143 restrains into granule to add dextrin and micropowder silica gel.Gross weight is 11236 grams.Therefrom take out 2250 grams, encapsulated according to 450 milligrams/amount, obtain 4966 capsules.All the other granules (to call embodiment 3 extracts in the following text) are used to carry out following experimental example test.
Every inspection is the same.
[experimental example]
Below antibacterial by the antiinflammatory of extract of the present invention, antiallergic, function of promoting blood circulation to disperse blood clots prove the function of its treatment acne.
Test material:
1. be subjected to the reagent thing
Embodiment 3 gained granules, concentration is standby with the suspension that 0.5% carboxymethylcellulose sodium solution is mixed with the experiment desired concn as required.
2. other medicines
Dimethylbenzene (chemical pure): reagent one factory in Shanghai produces, lot number: 971201; Carrageenin: Inst., of Clinical Pharmacology, Anhui Medical Univ. provides; 4-aminopyridine (4-Aminopyridin): Merck product; Sodium carboxymethyl cellulose (CMC-Na): Shanghai China reagent portion of doctor company; Coal tar: provide (100%) by Hefei City Gas Company, show black, pasty state; Salad oil: the vegetable oil company limited was produced in Qingdao was good, little Huang, and clear and bright, other medicine is commercially available qualified medicine.
3. strain
Streptococcus pneumoniae (3409), staphylococcus aureus (ATCC25923), propionibacterium acne (65102) are provided by Nat'l Pharmaceutical ﹠ Biological Products Control Institute, are reference culture.
4. culture medium
Propionibacterium acne is adopted the meat soup that contains 10% fresh calf serum; Streptococcus pneumoniae is adopted the meat soup of 5% fresh defiber Sanguis caprae seu ovis; Staphylococcus aureus is directly adopted meat soup; All self-controls.
5. experimental animal
Kunming mouse, the Wistar rat is provided by Anhui Prov. Medical Science Inst.; Rabbit is provided by laboratory animal section of Bengbu Medical College.
6. experiment condition: 18～28 ℃ of room temperatures, humidity 60～75%
7. test method and result
(1) inhibitory action of the mice ear due to the present composition xylol
Get 55 of male mices, volume 21.0 ± 3.0g, be divided into 5 groups at random, every group 11, capacity 0.5%CMC such as difference orally give, be equivalent to 1.0g crude drug/kg, 3.0g crude drug/kg, 9.0g embodiment 3 extracts of crude drug/kg, successive administration 3 days, 40min causes inflammation after the last administration, is just embrocating left ear with dimethylbenzene, reverse side, 0.1ml/ Mus, auris dextra compares, and causes scorching back 30min and puts to death animal, gets auricle with Φ 9mm card punch in the ears antimere, weigh respectively in precision balance, left and right sides auricle weight difference is the swelling degree, and the difference of comparative drug group and matched group is calculated and pressed down swollen rate.
Figure C20031011799400161
The results are shown in Table 1.
Table 1 present composition extract xylol causes the influence of mice ear
Group Dosage (g/kg) Number of animals (n) The swelling degree (mg, x ± s) Press down swollen rate (%)
CMC 11 21.47±2.48
The extract small dose group 1.0 11 14.65±3.04 ** 31.77
Dosage group in the extract 3.0 11 11.96±2.59 ** 44.29
The heavy dose of group of extract 9.0 11 10.97±3.01 ** 48.91
Compare with the CMC group *P＜0.01
From last table, the extract of the present composition can obviously suppress dimethylbenzene induced mice ear inflammation, and presents dose-effect relationship preferably.
(2) present composition on Carrageenan causes the influence of rat paw edema
Get 50 of male rats, body weight 200 ± 20.0g, be divided into 5 groups at random, every group 10, capacity 0.5%CMC such as difference orally give, be equivalent to 0.7g crude drug/kg (small dose group), 2.0g crude drug/kg (middle dosage group), 6.0g embodiment 3 extracts of crude drug/kg (heavy dose of group), successive administration 3 days, 40min causes inflammation after the last administration, at the subcutaneous injection 1% carrageenin 0.1ml/ foot of the right back sufficient sole of the foot portion of rat, causing scorching back 1h respectively, 2h, 3h, 4h, 5h measures hind leg volume under the ankle joint with sufficient pawl volume conduction analyzer (Shandong Medical University's product), and with cause scorching front foot pawl volumetric ratio, calculate cause scorching before and after the difference of sufficient pawl volume be the swelling degree, the results are shown in Table 2.
Table 2 extract on Carrageenan of the present invention causes the influence of rat paw edema
Figure C20031011799400171
Compare with the CMC group *P＜0.05, *P＜0.01
As seen from Table 2, the present composition can obviously suppress the inflammatory reaction that carrageenin causes rat paw edema.
(3) the mice 4-aminopyridine is caused the inhibitory action of itching and reacting
Get 50 of Kunming mouses, body weight 18～24g, the male and female dual-purpose, be divided into 5 groups at random, every group 10, the oral 0.5%CMC of difference, be equivalent to 1.0g crude drug/kg (small dose group), 3.0g crude drug/kg (middle dosage group), 9.0g embodiment 3 extracts of crude drug/kg (heavy dose of group), successive administration 3 days, 40min (is made into 0.5% solution with normal saline in mouse carotid back subcutaneous injection 4-aminopyridine 1mg/kg after the last administration, be diluted to 0.01% solution with normal saline before the experiment), mice is put into mouse cage, what mice in 10 minutes given behind the 4-aminopyridine in record licks body number of times (lick precursor reactant: 10s left and right sides mice occurs turning one's head repeatedly licking the behavior at its back after injecting 4-aminopyridine, licks body continuously with mice and makes to lick body and calculate for 1 time to minibreak occurring).The results are shown in Table 3.
Figure C20031011799400181
Table 3 present composition causes the influence that mice licks precursor reactant to 4-aminopyridine
Group Dosage (g/kg) Lick in 10 minutes the body number (inferior, x ± s) Suppression ratio (%)
CMC 35.30±10.81
Small dose group 1.0 28.90±9.02 * 18.13
Middle dosage group 3.0 18.60±7.00 ** 47.31
Heavy dose of group 9.0 16.90±5.92 ** 52.12
Compare with the CMC group *P＜0.05 *P＜0.01
As seen from Table 3, the present composition causes the reaction of itching to the mice 4-aminopyridine the obvious suppression effect, and its itching-relieving action has good dose-effect relationship.
(4) to the inhibitory action of external propionibacterium acne
Adopt the experiment of micro-culture method, with distilled water embodiment 3 extracts are mixed be equivalent to 0.48,0.24,0.12,0.06,0.03, the solution of 0.015g crude drug/ml, adjust PH=7.2 respectively, boiled 30 minutes, sterilization, 4 ℃ of refrigerators are preserved standby.Get 96 porocyte culture plates, add variable concentrations solution respectively, 100 μ l/ holes add 3 days the propionibacterium acne liquid 100 μ l/ holes of cysticercosis cellulosae cutis base cultivation by 100 times of dilutions again, respectively establish 3 multiple holes, establish medicinal liquid contrast and the contrast of bacterium liquid simultaneously, put in the vacuum desiccator, negative pressure of vacuum reaches 0.1mpa, cultivates 7 days for 37 ℃, observation has or not colony growth, and the highest drug dilution degree that bacterium colony is not grown is the minimum inhibitory concentration (MIC) of this medicine.The results are shown in Table 4.The present composition is 0.12 milligram of crude drug/milliliter to propionibacterium acne liquid minimum inhibitory concentration.
(5) present composition is to the inhibitory action of streptococcus pneumoniae, staphylococcus aureus
Adopt liquid tube method, get some of sterilization small test tubes, by reagent, concentration is respectively embodiment 3 extracts and is equivalent to 0.024,0.012,0.006,0.003,0.0015,0.00075g crude drug/ml with corresponding culture medium dilution; Make concentration with Coming-of-Age Day taking dose multiple proportions relation equate, every pipe dress 2ml, the fresh bacterium liquid of adding 0.1ml, making every pipe finally contain bacteria concentration is 5 * 10 5CFU/ml puts to cultivate in 37 ℃ of incubators to observe behind the 18h and has or not colony growth.The highest drug dilution degree that bacterium colony is not grown the results are shown in Table 4 for the minimum inhibitory concentration (MIC) of this medicine.
Table 4 present composition bacteriostatic test result
Figure C20031011799400191
Annotate: " " expression asepsis growth, " " expression has bacteria growing, N " the expression undetermined
As seen from Table 4, the flat Cuo capsule that detoxifies has bacteriostasis preferably to streptococcus pneumoniae, staphylococcus aureus, and propionibacterium acne is had certain inhibitory action.
(6) present composition is to the refrigeration function of rat fever due to the beer yeast
50 of rats are used in experiment; body weight 200 ± 20.0g; the male and female dual-purpose; be divided into 5 groups at random; every group 10; the oral 0.5%CMC of difference; be equivalent to 0.7g crude drug/kg (small dose group); 2.0g crude drug/kg (middle dosage group); 6.0g embodiment 3 extracts of crude drug/kg (heavy dose of group), 1 time/day, successive administration 3 days; surveyed administration after its arm's length basis anus temperature on the 3rd day; 30min behind the medicine, each Corium Mus be injection 7% beer yeast suspension 0.5ml/100g down, per hour surveys the anus temperature then 1 time; continuous 5 hours; the body temperature of respectively organizing the rat different time changes, and is surveyed anus temperature and basic anus using warming therapy difference with different time, is the index of body temperature variation.The results are shown in Table 5.
Table 5 present composition is to the influence of beer yeast pyrogenicity rat temperature
Figure C20031011799400201
Compare with the CMC group *P＜0.05, *P＜0.01
As seen from Table 5, the basic anus temperature zero difference of each treated animal, injection beer yeast suspension can make fervescence, and dosage promptly shows the better antipyretic effect in the present composition.With heavy dose group no significant difference.
(7) to the hemorheology of rabbit ear acne model and the influence of serum testosterone
Get 40 of male and healthy white rabbits, body weight 2.5～3.0kg, be coated with coal tar 1 time in each rabbit auris dextra medial surface syrinx opening part every day, embrocates the about 2 * 2cm of area 2, left ear relevant position is coated with salad oil, continuous 2 weeks, the variation of the perusal rabbit ear, then experimental rabbit is divided into 5 groups at random, 8 every group, the oral 0.5%CMC of difference, embodiment 3 extracts that are equivalent to 0.36g crude drug/kg (small dose group), 1.1g crude drug/kg (middle dosage group), 3.3g crude drug/kg (heavy dose of group), 1 time/day, successive administration 14 days was done perusal to the rabbit ear on the 15th day, blood examination, serum testosterone assay are carried out in blood sampling.Downcut the auricle histological examination.
Blood examination the results are shown in Table 6.
Table 6 present composition is to the hemorheological influence of experimental acne rabbit
Compare with the CMC group *P＜0.05, *P＜0.01
As seen from Table 6, the present composition significantly decreases to experimental acne rabbit blood rheology index plasma viscosity matter, erythrocyte sedimentation rate value, celloglobulin, has compared extremely significant difference with the CMC group, illustrates that the present composition has good function of promoting blood circulation to disperse blood clots.
(8) to the influence of the pathological change of rabbit ear acne model
(8-1) perusal
The normal rabbit ear is poor, soft, visible blood capillary clearly on it, the visible follicular orifice marshalling in rabbit ear tube opening place; Be coated with coal tar 2 all rabbit ear thickness increases, hardening, the follicular orifice protuberance has the blackhead shape, be coated with the salad oil rabbit ear and do not have significant change, after the two weeks, be coated with the coal tar rabbit ear and observe as seen, the still visible significantly hair follicle keratotic plug of people CMC group, but the follicular orifice protuberance slightly alleviates, flatten, obviously reduce for flat Cuo capsule of detoxifcation and the visible local hair follicle keratotic plug of QINGRE ANCHUANG PIAN group, leave over the hair follicle of pitting after part has bolt to come off, the follicular orifice protuberance flattens, and obviously reduces.
(8-2) histological examination
(8-2-1) histological examination method: get auricle coating place skin biopsy, with 10% formalin fixed, embedding, section HE dyeing, tissues observed changes under light microscopic.
(8-2-2) histology's criterion: (0) no acne, do not see inflammatory cell in the visual field; (1) be the visible a small amount of fine and close keratinization material of infundibulum of hair follicle portion, inflammatory cell is not obvious; (2) be infundibulum of hair follicle portion visible moderate keratinization material, and extend that a large amount of inflammatory cells are arranged to sebaceous gland; (3) in the hair follicle for expansion keratinization material is widely arranged, inflammatory cell is full of the visual field.
(8-2-3) histological observation result: the salad oil ear is not seen keratinization material and inflammatory cell; And the CMC rabbit ear keratinization material that is coated with coal tar is full of hair follicle, kitchen range squamous epithelial cancer propelling property hypertrophy downwards, and companion's horn plug forms, and a matter has lymphocytic infiltration; The medication group: granular layer, acantholysis cell be hypertrophy or not obvious hypertrophy slightly, and inflammatory cell is not obvious, and similar to the positive controls performance.The result sees table 7 for details.
Table 7 present composition is to histology's influence of experimental rabbit acne model
Figure C20031011799400221
Compare with model control group: *P＜0.05 *P＜0.01
As seen from Table 7, the present composition causes the experimental acne model of the rabbit ear to coal tar therapeutical effect, can reduce its hairy cyst, can make inflammatory cell not obvious.

Claims (3)

1. treat the pharmaceutical composition of acne, its by Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Artemisiae Scopariae and Pericarpium Citri Reticulatae with 15: 10: 10: 12: 12: 10: the extract that 15: 10 ratio obtains was formed, wherein, described extract is prepared respectively by the following method by Radix Scutellariae, Rhizoma Coptidis, Radix Sophorae Flavescentis, Fructus Forsythiae, Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Herba Artemisiae Scopariae and Pericarpium Citri Reticulatae:
Radix Scutellariae decocts with water 3 times, and each 2 hours, collecting decoction, filter, filtrate is concentrated between relative density 1.05-1.10, regulates pH value 1.5-2.0, and 70 ℃ are incubated 30 minutes, left standstill 24 hours, sucking filtration precipitates with washing with alcohol to pH value 5.0-6.5, after the washing of reuse low amounts of water, drying under reduced pressure is ground into fine powder;
Rhizoma Coptidis, Radix Sophorae Flavescentis two flavors add 60% alcohol reflux 3 times, and each 1.5 hours, filter, filtrate recycling ethanol, and be condensed into dry extract, be ground into fine powder;
Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati two flavors add 90% ethanol extraction 2 times, and each 1.5 hours, filter, medicinal residues are standby, merge ethanol extract, decompression recycling ethanol, and be condensed into dry extract, be ground into fine powder; With
Pericarpium Citri Reticulatae, Fructus Forsythiae, Herba Artemisiae Scopariae are extracted volatile oil with vapor distillation respectively, and use beta-cyclodextrin inclusion compound; Aqueous solution after distillation device is in addition collected; Medicinal residues and above-mentioned medicinal residues merge, and decoct with water each 1.5 hours 2 times, filter, filtrate and above-mentioned aqueous solution merge, and being concentrated into relative density is 1.10 clear paste, add ethanol and make and contain the alcohol amount and reach 60%, stir evenly, 2-8 ℃ left standstill 24 hours, filtered filtrate recycling ethanol, and be condensed into dry extract, be ground into fine powder.
2. according to the pharmaceutical composition of claim 1, it is characterized in that this pharmaceutical composition is made into powder, tablet, piece agent or granule, medicinal tea or capsule.
3. according to the pharmaceutical composition of claim 2, it is characterized in that this pharmaceutical composition is made into capsule.
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