CN100526932C - 荧光显微镜装置以及确定样品中荧光标记物的方法 - Google Patents

荧光显微镜装置以及确定样品中荧光标记物的方法 Download PDF

Info

Publication number
CN100526932C
CN100526932C CNB2005800043133A CN200580004313A CN100526932C CN 100526932 C CN100526932 C CN 100526932C CN B2005800043133 A CNB2005800043133 A CN B2005800043133A CN 200580004313 A CN200580004313 A CN 200580004313A CN 100526932 C CN100526932 C CN 100526932C
Authority
CN
China
Prior art keywords
field
sample
fluorescent
microscope
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2005800043133A
Other languages
English (en)
Chinese (zh)
Other versions
CN1918501A (zh
Inventor
B·格莱希
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Koninklijke Philips Electronics NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Electronics NV filed Critical Koninklijke Philips Electronics NV
Publication of CN1918501A publication Critical patent/CN1918501A/zh
Application granted granted Critical
Publication of CN100526932C publication Critical patent/CN100526932C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
CNB2005800043133A 2004-02-09 2005-01-27 荧光显微镜装置以及确定样品中荧光标记物的方法 Expired - Fee Related CN100526932C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04100462 2004-02-09
EP04100462.3 2004-02-09

Publications (2)

Publication Number Publication Date
CN1918501A CN1918501A (zh) 2007-02-21
CN100526932C true CN100526932C (zh) 2009-08-12

Family

ID=34833743

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2005800043133A Expired - Fee Related CN100526932C (zh) 2004-02-09 2005-01-27 荧光显微镜装置以及确定样品中荧光标记物的方法

Country Status (7)

Country Link
US (1) US20070161075A1 (enExample)
EP (1) EP1716444B1 (enExample)
JP (1) JP4804363B2 (enExample)
CN (1) CN100526932C (enExample)
AT (1) ATE418084T1 (enExample)
DE (1) DE602005011763D1 (enExample)
WO (1) WO2005076050A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060248944A1 (en) * 2003-04-15 2006-11-09 Koninklijke Philips Electronics N.V. Method to determine the spatial distribution of magnetic particles and magnetic particle administering compositions
DE102005017817A1 (de) * 2005-04-18 2006-10-26 Siemens Ag Verfahren und Vorrichtung zur Untersuchung eines biologischen Gewebes
WO2007015190A2 (en) * 2005-08-01 2007-02-08 Philips Intellectual Property & Standards Gmbh Optical imaging
JP2011022201A (ja) * 2009-07-13 2011-02-03 Jikei Univ 顕微鏡装置用の光源装置、及び光源装置付き観察試料載置具、並びに顕微鏡装置
EP3955042B1 (en) 2013-08-26 2024-08-21 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
CN103575716A (zh) * 2013-11-15 2014-02-12 中国科学院长春应用化学研究所 磁场调控的超分辨荧光成像方法
CA2998829A1 (en) 2015-09-17 2017-03-23 S.D. Sight Diagnostics Ltd Methods and apparatus for detecting an entity in a bodily sample
EP3436864B1 (en) 2016-03-30 2021-04-28 S.D. Sight Diagnostics Ltd. Image processing device for identifying blood parasites
WO2017195205A1 (en) 2016-05-11 2017-11-16 S.D. Sight Diagnostics Ltd Sample carrier for optical measurements
AU2017263807B2 (en) 2016-05-11 2023-02-02 S.D. Sight Diagnostics Ltd Performing optical measurements on a sample
JP7214729B2 (ja) 2017-11-14 2023-01-30 エス．ディー．サイト ダイアグノスティクス リミテッド 光学測定用試料収容器
DE102018206406C5 (de) 2018-04-25 2025-05-15 Carl Zeiss Meditec Ag Mikroskopiesystem
CA3155820A1 (en) 2019-10-22 2021-04-29 S.D. Sight Diagnostics Ltd Accounting for errors in optical measurements
WO2021116962A1 (en) 2019-12-12 2021-06-17 S.D. Sight Diagnostics Ltd Artificial generation of color blood smear image
CA3160695A1 (en) 2019-12-12 2021-06-17 S.D. Sight Diagnostics Ltd Analyzing an analyte disposed within a medium

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807677A (en) * 1995-03-10 1998-09-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for direct identification of few nucleic acid strands
WO2002059582A1 (de) * 2001-01-25 2002-08-01 Gnothis Holding Sa Verfahren zur fluoreszenzspektroskopischen, insbesondere fluoreszenzkorrelationsspektroskopischen untersuchung einer messprobe sowie einrichtung zur durchführung desselben
US20030085703A1 (en) * 2001-10-19 2003-05-08 Bernhard Gleich Method of determining the spatial distribution of magnetic particles

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4578167A (en) * 1982-09-28 1986-03-25 Biofusion, Inc. Cell fusion
US4676640A (en) * 1984-09-12 1987-06-30 Syntex (U.S.A.) Inc. Fluctuation analysis for enhanced particle detection
MY139225A (en) * 1998-02-26 2009-08-28 Anglo Operations Ltd Method and apparatus for separating particles
DE19903001A1 (de) * 1999-01-26 2000-08-24 Evotec Biosystems Ag Verfahren und Vorrichtung zur Detektion mikroskopisch kleiner Objekte
US6271660B1 (en) * 1999-06-11 2001-08-07 Mts Sytems Corporation Permanent multi-pole position magnet
US6437551B1 (en) * 1999-11-02 2002-08-20 The Regents Of The University Of California Microfabricated AC impedance sensor
JP4994560B2 (ja) * 2000-08-25 2012-08-08 アムニス コーポレイション 細胞などの小さな移動物体の速度測定
US20020127144A1 (en) * 2001-03-08 2002-09-12 Mehta Shailesh P. Device for analyzing particles and method of use
US7968305B2 (en) * 2001-03-24 2011-06-28 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
JP4890242B2 (ja) * 2003-04-15 2012-03-07 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 検査エリアにおける状態変数の空間分解を決定する装置及び方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807677A (en) * 1995-03-10 1998-09-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for direct identification of few nucleic acid strands
WO2002059582A1 (de) * 2001-01-25 2002-08-01 Gnothis Holding Sa Verfahren zur fluoreszenzspektroskopischen, insbesondere fluoreszenzkorrelationsspektroskopischen untersuchung einer messprobe sowie einrichtung zur durchführung desselben
US20030085703A1 (en) * 2001-10-19 2003-05-08 Bernhard Gleich Method of determining the spatial distribution of magnetic particles

Also Published As

Publication number Publication date
CN1918501A (zh) 2007-02-21
JP4804363B2 (ja) 2011-11-02
US20070161075A1 (en) 2007-07-12
DE602005011763D1 (de) 2009-01-29
EP1716444A1 (en) 2006-11-02
ATE418084T1 (de) 2009-01-15
WO2005076050A1 (en) 2005-08-18
EP1716444B1 (en) 2008-12-17
JP2007522502A (ja) 2007-08-09

Similar Documents

Publication Publication Date Title
CN100526932C (zh) 荧光显微镜装置以及确定样品中荧光标记物的方法
Glenn et al. Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours
Ghanam et al. Single molecule localization microscopy for studying small extracellular vesicles
Fu et al. Characterization and application of single fluorescent nanodiamonds as cellular biomarkers
Rittweger et al. STED microscopy reveals crystal colour centres with nanometric resolution
Chen et al. Wide-field multispectral super-resolution imaging using spin-dependent fluorescence in nanodiamonds
Prigozhin et al. Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy
Nienhaus et al. Where do we stand with super-resolution optical microscopy?
Fokkema et al. Fluorescently labelled silica coated gold nanoparticles as fiducial markers for correlative light and electron microscopy
Liu et al. Tempo-spatially resolved scattering correlation spectroscopy under dark-field illumination and its application to investigate dynamic behaviors of gold nanoparticles in live cells
Fleischer et al. Optically activated delayed fluorescence
Barbiero et al. Nanoscale magnetic imaging enabled by nitrogen vacancy centres in nanodiamonds labelled by iron–oxide nanoparticles
Yang et al. Sub-diffraction imaging of nitrogen-vacancy centers in diamond by stimulated emission depletion and structured illumination
JP2007522502A5 (enExample)
Nawa et al. Multi‐color imaging of fluorescent nanodiamonds in living HeLa cells using direct electron‐beam excitation
Mi et al. Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy
Robinson et al. Optically detected magnetic resonance for selective imaging of diamond nanoparticles
Castelletto et al. Color centers in wide-bandgap semiconductors for subdiffraction imaging: a review
Li et al. Pulsed saturated absorption competition microscopy on nonbleaching nanoparticles
Myers et al. Recent advances in imaging subcellular processes
Xu et al. Super-resolution enabled widefield quantum diamond microscopy
Furukawa et al. Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy
Yoshida et al. Super-resolution fluorescence imaging of extracellular environments
Iwasaki et al. Recent technological advances in correlative light and electron microscopy for the comprehensive analysis of neural circuits
Kim et al. Quantifying translational mobility in neurons: comparison between current optical techniques

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090812

Termination date: 20120127