JP4804363B2 - 蛍光顕微鏡装置 - Google Patents
蛍光顕微鏡装置 Download PDFInfo
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- JP4804363B2 JP4804363B2 JP2006551974A JP2006551974A JP4804363B2 JP 4804363 B2 JP4804363 B2 JP 4804363B2 JP 2006551974 A JP2006551974 A JP 2006551974A JP 2006551974 A JP2006551974 A JP 2006551974A JP 4804363 B2 JP4804363 B2 JP 4804363B2
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- 239000003550 marker Substances 0.000 claims abstract description 33
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- 125000002066 L-histidyl group Chemical group [H]N1C([H])=NC(C([H])([H])[C@](C(=O)[*])([H])N([H])[H])=C1[H] 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Description
−前記サンプルから蛍光放射線を励起させ、撮像する蛍光顕微鏡であり、本目的に適した顕微鏡は蛍光顕微鏡の分野から知られている蛍光顕微鏡、及び
−空間的に不均一な磁場及び/又は空間的に不均一な電場をサンプル内に発生させる場発生器であり、この場の不均一さは少なくとも局所的な領域に存在しなければならない場発生器、
を含んでいる。
−前記サンプル内に不均一な磁場及び/又は不均一な電場を一時的に静止又は変化を発生させるので、前記蛍光マーカが局所的に異なる状態となるステップ、
−例えば適当な量子エネルギーの一次放射線によりサンプル内の蛍光放射線を励起するステップ、
−前記サンプルから来る蛍光放射線の少なくとも1つの光学画像を生成するステップ、及び
−前記少なくとも1つの上述した画像及び不均一な場の既知の関連する強度分布を用いて、蛍光マーカの空間分布を計算するステップであり、好ましくは、前記計算は、空間的に異なる場の強度分布の場合、少なくとも2つの画像に基づき、この場の強度分布は、ポイント形の領域又はピクセル/ボクセルの分析を各々可能にする場合、通常はN個の異なる場の強度分布がN個のピクセル/ボクセルの表示するのに必要とされるステップ
を有する。
N.Kh. Petrov, V.N. Borisenko, A.V.Starostin, M.V.Alfimov著、"Polar molecular clusters produced upon photoinduced electron transfer in an intermolecular exciplex in binary solvents" J. Phys. Chem., 1992, vol.96, no.7, p.2901;
N.Kh. Petrov, V.N. Borisenko, M.V.Alfimov著、"Study of preferential salvation in binary solvent mixtures by the fluorescence-detected magnetic field effect" J. Chem. Soc., Faraday Trans., 1994, vol.90, no.1, 109-111;N.Kh. Petrov, V.N. Borisenko, M.V.Alfimov著、"Magnetic field Effects of Exciplex Fluorescence of the Pyrene-Azacrown Ether System in the Presence of Alkali and Alkaline Earth Salts" J. Chem. Soc. Mendeleev Commun. 1995;N.Kh. Petrov, V.N. Borisenko, M.V.Alfimov, T. Fiebig, H. Staerk著、"Fluorescene-detected Magnetic Field Effects in Exciplex Systems Containing Azacrown Ethers as Electron Donor" J. Phys. Chem.., 1996, vol.100, no.16, 6368-6370、を参照することもできる。
Claims (10)
- 磁気及び/又は電気感受性の蛍光特性をもつ蛍光マーカを含んでいるサンプルを撮像するための顕微鏡装置において、
−前記サンプルからの蛍光放射線を励起すると共に、撮像するための蛍光顕微鏡、及び
−前記サンプル内に不均一な磁場及び/又は不均一な電場を発生させる場発生器であって、最小の磁場及び/又は電場強度を示す焦点領域が形成されるように前記不均一な磁場及び/又は電場が生成される、場発生器
を有する顕微鏡装置。 - 前記サンプル内の前記不均一な場を変更するように構成される請求項1に記載の顕微鏡装置。
- 不均一な場を発生させる前記場発生器は、第1の極性からなる第1の極体を有し、前記第1の極体は、少なくともその両側に異なる極性からなる第2の極体が隣接していることを特徴とする請求項1に記載の顕微鏡装置。
- 請求項1に記載の顕微鏡装置において、1つ又は好ましい数の記録中に、前記不均一な場の既知の空間強度分布から、前記サンプル内の前記蛍光マーカの分布を再現するように構成される、前記蛍光顕微鏡により記録される前記画像の画像処理を行うためのデータ処理装置を有することを特徴とする顕微鏡装置。
- サンプル内の磁気及び/又は電気感受性の蛍光特性をもつ蛍光マーカの前記空間分布を決める方法において、
−前記サンプル内に不均一な磁場及び/又は不均一な電場を発生させるステップであって、最小の磁場及び/又は電場強度を示す焦点領域が形成されるように前記不均一な磁場及び/又は電場が生成される、ステップ、
−前記サンプル内に蛍光放射線を励起するステップ、
−蛍光顕微鏡を用いて、前記サンプルから来る前記蛍光放射線の画像を生成するステップ、及び
−前記生成した画像を用いると共に、前記場の既知の強度分布を用いて前記蛍光マーカの前記空間分布を計算するステップ
を有する方法。 - 前記不均一な磁場は、少なくとも102T/mの勾配を有することを特徴とする請求項5に記載の方法。
- 前記不均一な電場は、少なくとも1011V/m2の勾配を有することを特徴とする請求項5に記載の方法。
- 前記不均一な場は、場の強度の極小値をもつ無場ポイント又は領域を有することを特徴とする請求項5に記載の方法。
- 前記極小値の幅は、前記蛍光顕微鏡の光学解像度よりも小さいことを特徴とする請求項8に記載の方法。
- 前記サンプルは前記蛍光マーカを用いて溶液中において位置特定されることを特徴とする請求項5に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP04100462 | 2004-02-09 | ||
EP04100462.3 | 2004-02-09 | ||
PCT/IB2005/050354 WO2005076050A1 (en) | 2004-02-09 | 2005-01-27 | Fluorescence microscope arrangement |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2007522502A JP2007522502A (ja) | 2007-08-09 |
JP2007522502A5 JP2007522502A5 (ja) | 2011-04-21 |
JP4804363B2 true JP4804363B2 (ja) | 2011-11-02 |
Family
ID=34833743
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Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006551974A Expired - Fee Related JP4804363B2 (ja) | 2004-02-09 | 2005-01-27 | 蛍光顕微鏡装置 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20070161075A1 (ja) |
EP (1) | EP1716444B1 (ja) |
JP (1) | JP4804363B2 (ja) |
CN (1) | CN100526932C (ja) |
AT (1) | ATE418084T1 (ja) |
DE (1) | DE602005011763D1 (ja) |
WO (1) | WO2005076050A1 (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5010914B2 (ja) * | 2003-04-15 | 2012-08-29 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 磁性粒子の空間分布を決める方法及び磁性粒子を投与する組成物 |
DE102005017817A1 (de) * | 2005-04-18 | 2006-10-26 | Siemens Ag | Verfahren und Vorrichtung zur Untersuchung eines biologischen Gewebes |
WO2007015190A2 (en) * | 2005-08-01 | 2007-02-08 | Philips Intellectual Property & Standards Gmbh | Optical imaging |
JP2011022201A (ja) * | 2009-07-13 | 2011-02-03 | Jikei Univ | 顕微鏡装置用の光源装置、及び光源装置付き観察試料載置具、並びに顕微鏡装置 |
CN103575716A (zh) * | 2013-11-15 | 2014-02-12 | 中国科学院长春应用化学研究所 | 磁场调控的超分辨荧光成像方法 |
EP3350644B1 (en) | 2015-09-17 | 2021-04-28 | S.D. Sight Diagnostics Ltd. | Methods and apparatus for detecting an entity in a bodily sample |
US11733150B2 (en) | 2016-03-30 | 2023-08-22 | S.D. Sight Diagnostics Ltd. | Distinguishing between blood sample components |
EP3455610B1 (en) | 2016-05-11 | 2023-01-04 | S.D. Sight Diagnostics Ltd. | Sample carrier for optical measurements |
AU2018369859B2 (en) | 2017-11-14 | 2024-01-25 | S.D. Sight Diagnostics Ltd | Sample carrier for optical measurements |
DE102018206406B3 (de) * | 2018-04-25 | 2019-09-12 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Verfahren zum Betrieb eines Mikroskopiesystems |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08242858A (ja) * | 1995-03-10 | 1996-09-24 | Max Planck Ges Foerderung Wissenschaft Ev | 少数のヌクレイン酸鎖を直接証明する方法 |
US20040144929A1 (en) * | 2001-01-25 | 2004-07-29 | Lars Edman | Method for examining a test sample by means of fluorescence spectroscopy, especially flourescence correlatin spectroscopy, and device for carrying out said method |
Family Cites Families (11)
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US4578167A (en) * | 1982-09-28 | 1986-03-25 | Biofusion, Inc. | Cell fusion |
US4676640A (en) * | 1984-09-12 | 1987-06-30 | Syntex (U.S.A.) Inc. | Fluctuation analysis for enhanced particle detection |
MY139225A (en) * | 1998-02-26 | 2009-08-28 | Anglo Operations Ltd | Method and apparatus for separating particles |
DE19903001A1 (de) * | 1999-01-26 | 2000-08-24 | Evotec Biosystems Ag | Verfahren und Vorrichtung zur Detektion mikroskopisch kleiner Objekte |
US6271660B1 (en) * | 1999-06-11 | 2001-08-07 | Mts Sytems Corporation | Permanent multi-pole position magnet |
US6437551B1 (en) * | 1999-11-02 | 2002-08-20 | The Regents Of The University Of California | Microfabricated AC impedance sensor |
AU2001290568A1 (en) * | 2000-08-25 | 2002-03-04 | Amnis Corporation | Measuring the velocity of small moving objects such as cells |
US20020127144A1 (en) * | 2001-03-08 | 2002-09-12 | Mehta Shailesh P. | Device for analyzing particles and method of use |
WO2002077259A2 (en) * | 2001-03-24 | 2002-10-03 | Aviva Biosciences Corporation | Biochips including ion transport detecting structures and methods of use |
DE10151778A1 (de) * | 2001-10-19 | 2003-05-08 | Philips Corp Intellectual Pty | Verfahren zur Ermittlung der räumlichen Verteilung magnetischer Partikel |
CN1774200B (zh) * | 2003-04-15 | 2010-07-28 | 皇家飞利浦电子股份有限公司 | 对检测区域内的状态变量进行空间解像测定的装置及方法 |
-
2005
- 2005-01-27 CN CNB2005800043133A patent/CN100526932C/zh not_active Expired - Fee Related
- 2005-01-27 JP JP2006551974A patent/JP4804363B2/ja not_active Expired - Fee Related
- 2005-01-27 US US10/597,667 patent/US20070161075A1/en not_active Abandoned
- 2005-01-27 EP EP05702809A patent/EP1716444B1/en not_active Not-in-force
- 2005-01-27 AT AT05702809T patent/ATE418084T1/de not_active IP Right Cessation
- 2005-01-27 DE DE602005011763T patent/DE602005011763D1/de active Active
- 2005-01-27 WO PCT/IB2005/050354 patent/WO2005076050A1/en not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08242858A (ja) * | 1995-03-10 | 1996-09-24 | Max Planck Ges Foerderung Wissenschaft Ev | 少数のヌクレイン酸鎖を直接証明する方法 |
US20040144929A1 (en) * | 2001-01-25 | 2004-07-29 | Lars Edman | Method for examining a test sample by means of fluorescence spectroscopy, especially flourescence correlatin spectroscopy, and device for carrying out said method |
Also Published As
Publication number | Publication date |
---|---|
JP2007522502A (ja) | 2007-08-09 |
WO2005076050A1 (en) | 2005-08-18 |
CN1918501A (zh) | 2007-02-21 |
CN100526932C (zh) | 2009-08-12 |
EP1716444B1 (en) | 2008-12-17 |
US20070161075A1 (en) | 2007-07-12 |
EP1716444A1 (en) | 2006-11-02 |
DE602005011763D1 (de) | 2009-01-29 |
ATE418084T1 (de) | 2009-01-15 |
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